Пестицидные гены и способы их применения - RU2738424C2
Код документа: RU2738424C2
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ОБЛАСТЬ ТЕХНИКИ
[0001] Настоящее изобретение относится к способам и композициям для контроля вредителей, особенно вредителей растений.
ПЕРЕКРЕСТНАЯ ССЫЛКА НА РОДСТВЕННУЮ ЗАЯВКУ
[0002] По настоящей заявке испрашивается приоритет предварительной заявки США сериальный № 62/151156, поданной 22 апреля 2015 г., содержание этой заявки включено в настоящий документ посредством ссылки во всей полноте.
ССЫЛКА НА СПИСОК ПОСЛЕДОВАТЕЛЬНОСТЕЙ, ПОДАННЫЙ В ВИДЕ ТЕКСТОВОГО ФАЙЛА С ПОМОЩЬЮ EFS-WEB
[0003] Официальная копия списка последовательностей представлена в электронном виде посредством EFS-Web в виде списка последовательностей в формате ASCII с помощью файла под названием AgB012.PCT_seq_listing.txt, созданного 13 апреля 2016 г. и имеющего размер 1,5 MB, и подана одновременно с описанием изобретения. Список последовательностей, содержащийся в этом документе в формате ASCII, является частью описания изобретения и включен в настоящий документ посредством ссылки во всей полноте.
УРОВЕНЬ ТЕХНИКИ
[0004] Вредители, заболевания растений и сорняки могут быть серьезной угрозой для сельскохозяйственных культур. Потери из-за вредителей и заболеваний оцениваются в 37% от мирового сельскохозяйственного производства, причем 13% из-за насекомых, бактерий и других организмов.
[0005] Токсины являются детерминантами вирулентности, которые играют важную роль в патогенности микробов и/или их уклонении от иммунного ответа хозяина. В качестве инсектицидных белков использовались токсины из грамположительной бактерии Bacillus, особенно Bacillus thuringiensis. Современные стратегии используют гены, экспрессирующие эти токсины, для получения трансгенных культур. Трансгенные культуры, экспрессирующие инсектицидные белковые токсины, используются для борьбы с повреждением культур насекомыми.
[0006] Хотя использование токсинов Bacillus для контроля насекомых было успешным, у некоторых целевых вредителей во многих частях мира, в которых интенсивно использовались такие токсины, развилась резистентность к Bt-токсинам. Одним из способов решения этой проблемы является высевание Bt-культур с чередующимися рядами обычных не Bt-культур (убежище). Альтернативным способом предотвращения или замедления развития резистентности насекомых является пирамидирование в трансгенных растениях инсектицидных генов с различными способами воздействия на насекомых. Современная стратегия использования трансгенных культур, экспрессирующих инсектицидные белковые токсины, заключается в обращении особенного внимания на обнаружении новых токсинов помимо уже полученных из бактерии Bacillus thuringiensis. Эти токсины могут оказаться полезными в качестве альтернативы полученным из B. thuringiensis для применения в резистентных к насекомым и вредителям трансгенных растениях. Таким образом, необходимы новые белковые токсины.
КРАТКОЕ ОПИСАНИЕ ФИГУР
[0007] На фигуре 1 показано выравнивание SEQ ID NO: 177, 307, 372, 374 и 396. Различающиеся аминокислоты выделены серым.
[0008] На фигуре 2 показана взаимосвязь общей идентичности последовательностей между SEQ ID NO: 177, 307, 372, 374 и 396.
СУЩНОСТЬ ИЗОБРЕТЕНИЯ
[0009] Предлагаются композиции, обладающие пестицидной активностью, и способы их применения. Композиции включают в себя выделенные и рекомбинантные полипептидные последовательности, обладающие пестицидной активностью, рекомбинантные и синтетические молекулы нуклеиновой кислоты, кодирующие данные пестицидные полипептиды, конструкции ДНК, содержащие данные молекулы нуклеиновой кислоты, векторы, содержащие данные молекулы нуклеиновой кислоты, клетки-хозяева, содержащие данные векторы, и антитела к данным пестицидным полипептидам. Нуклеотидные последовательности, кодирующие полипептиды, предлагаемые в настоящем документе, можно использовать в конструкциях ДНК или кассетах экспрессии для трансформации и экспрессии в представляющих интерес организмах, включая микроорганизмы и растения.
[0010] Композиции и способы, предлагаемые в настоящем документе, применяются для получения организмов с улучшенной резистентностью или толерантностью к вредителям. Эти организмы и композиции, содержащие данные организмы, являются желательными для сельскохозяйственных целей. Также предлагаются трансгенные растения и семена, содержащие нуклеотидную последовательность, которая кодирует пестицидный белок настоящего изобретения. Такие растения являются резистентными к насекомым и другим вредителям.
[0011] Предлагаются способы получения различных полипептидов, раскрытых в настоящем документе, и использования этих полипептидов для контроля или уничтожения вредителей. Также включены способы и наборы для детектирования полипептидов настоящего изобретения в образце.
ПОДРОБНОЕ ОПИСАНИЕ
[0012] Далее настоящие изобретения будут описаны более подробно со ссылкой на прилагаемые чертежи, на которых показаны некоторые, но не все варианты осуществления данных изобретений. Действительно, эти изобретения могут быть воплощены во многих различных формах и не должны истолковываться как ограниченные вариантами осуществления, приведенными в настоящем документе; скорее, эти варианты осуществления предоставляются для того, чтобы данное раскрытие удовлетворяло применимым юридическим требованиям. Подобные числа во всем документе относятся к одинаковым элементам.
[0013] Специалисту в области техники, к которой принадлежат эти изобретения, будет очевидно множество модификаций и других вариантов осуществления изобретений, приведенных в настоящем документе, благодаря идеям, представленным в вышеупомянутых описаниях и на соответствующих чертежах. Поэтому следует понимать, что настоящие изобретения не должны быть ограничены конкретными раскрытыми вариантами осуществления, и что предполагается, что модификации и другие варианты осуществления включены в объем прилагаемой формулы изобретения. Хотя в настоящем документе используются специальные термины, они используются только в общем и описательном смысле, а не с целью ограничения.
I. Полинуклеотиды и полипептиды
[0014] Предлагаются композиции и способ для придания организму пестицидной активности. Модифицированный организм проявляет резистентность или толерантность к пестицидам. Предлагаются рекомбинантные пестицидные белки или полипептиды и их фрагменты и варианты, которые сохраняют пестицидную активность, которые включают приведенные в SEQ ID NO: 1-398. Пестицидные белки являются биологически активными (например, пестицидными) в отношении вредителей, включая насекомых, грибы, нематоды и т.п. Нуклеотиды, кодирующие пестицидные полипептиды, включая, например, SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398 или их активные фрагменты или варианты, можно использовать для получения трансгенных организмов, таких как растения и микроорганизмы. Пестицидные белки являются биологически активными (например, пестицидными) в отношении вредителей, включая насекомых, грибы, нематоды и т.п. Полинуклеотиды, кодирующие пестицидные полипептиды, включая, например, SEQ ID NO: 1-398 или их активные фрагменты или варианты, можно использовать для получения трансгенных организмов, таких как растения и микроорганизмы. Трансформированные организмы характеризуются геномами, которые содержат по меньшей мере одну стабильно включенную конструкцию ДНК, содержащую кодирующую последовательность для пестицидного белка, раскрытого в настоящем документе. В некоторых вариантах осуществления кодирующая последовательность функционально связана с промотором, который управляет экспрессией кодируемого пестицидного полипептида. Соответственно, предлагаются трансформированные микроорганизмы, клетки растений, ткани растений, растения, семена и части растений. Сводная информация о различных полипептидах, их активных вариантах и фрагментах и кодирующих их полинуклеотидах, приведена ниже в таблице 1. Как отмечено в таблице 1, предлагаются различные формы полипептидов. Предлагаются полноразмерные пестицидные полипептиды, а также модифицированные версии исходной полноразмерной последовательности (т.е. варианты). В таблице 1 дополнительно отмечены последовательности "CryBP1". Такие последовательности содержат акцессорные полипептиды, которые могут быть связаны с некоторыми из генов токсинов. В таких случаях последовательности CryBP1 можно использовать отдельно или в комбинации с любыми из пестицидных полипептидов, предлагаемых в настоящем документе. Таблица 1 дополнительно предлагает полипептиды C-конца Split-Cry. Такие последовательности содержат последовательность расположенного по ходу транскрипции белка, который имеет гомологию с C-концевой областью генов токсинов класса Cry и обычно обнаруживается после гена Cry, который не является полноразмерным, и у которого отсутствует ожидаемая C-концевая область.
Таблица 1. Сводная информация о SEQ ID NO, классе гена и его вариантах
i. Классы пестицидных белков
[0015] Пестицидные белки, предлагаемые в настоящем документе, и нуклеотидные последовательности, кодирующие их, применяются в способах воздействия на вредителей. То есть композиции и способы настоящего изобретения находят применение в сельском хозяйстве для контроля или уничтожения вредителей, включая вредителей многих культурных растений. Пестицидные белки, предлагаемые в настоящем документе, представляют собой белковые токсины из бактерий и проявляют активность в отношении некоторых вредителей. Пестицидные белки относятся к нескольким классам токсинов, включая токсины Cry, Cyt, BIN и Mtx. Смотри, например, таблицу 1 для классификаций конкретных белков с различными SEQ ID NO, предлагаемых в настоящем документе. Кроме того, во всем настоящем раскрытии делается ссылка на записи базы данных Pfam. База данных Pfam представляет собой базу данных белковых семейств, каждая из которых представлена несколькими выравниваниями последовательностей и марковской моделью скрытого профиля. Finn et al. (2014) Nucl. Acid Res. Database Issue 42:D222-D230.
[0016] Bacillus thuringiensis (Bt) является грамположительной бактерией, которая производит инсектицидные белки в виде кристаллических включений во время своей фазы спорообразования при росте. Белковые включения Bacillus thuringiensis (Bt) называются кристаллическими белками или δ-эндотоксинами (или Cry-белками), которые токсичны для членов класса Insecta и других беспозвоночных. Аналогично, Cyt-белки представляют собой белки параспоральных включений из Bt, которые проявляют гемолитическую (цитолитическую) активность или обладают очевидным сходством последовательностей с известным Cyt-белком. Эти токсины высоко специфичны к своему целевому организму и безопасны для людей, позвоночных животных и растений.
[0017] Структура Cry-токсинов имеет пять консервативных аминокислотных блоков, сконцентрированных главным образом в центре домена или на стыке между доменами. Cry-токсин состоит из трех доменов, каждый с определенной функцией. Домен I представляет собой пучок из семи α-спиралей, в котором центральная спираль полностью окружена шестью внешними спиралями. Этот домен участвует в образовании каналов в мембране. Домен II выглядит как треугольная колонна из трех антипараллельных β-листов, которые схожи с антигенсвязывающими областями иммуноглобулинов. Домен III содержит антипараллельные β-цепи в форме β-сендвича. N-концевая часть белка токсина отвечает за его токсичность и специфичность и содержит пять консервативных областей. C-концевая часть обычно высококонсервативна и, вероятно, отвечает за образование кристаллов. Смотри, например, патент США № 8878007.
[0018] Штаммы B. thuringiensis демонстрируют широкий диапазон специфичности в отношении различных отрядов насекомых (Lepidoptera, Diptera, Coleoptera, Hymenoptera, Homoptera, Phthiraptera или Mallophaga и Acari) и других беспозвоночных (Nemathelminthes, Platyhelminthes и Sarocomastebrates). Cry-белки были классифицированы на группы на основании токсичности в отношении различных групп насекомых и беспозвоночных. Обычно Cry I демонстрирует токсичность в отношении чешуекрылых, Cry II в отношении чешуекрылых и двукрылых, CryIII в отношении жесткокрылых, Cry IV в отношении двукрылых, а Cry V и Cry VI в отношении нематод. Новые Cry-белки могут быть идентифицированы и отнесены к группе Cry на основании аминокислотной идентичности. Смотри, например, документы Bravo, A. (1997) J. of Bacteriol. 179:2793-2801; Bravo et al. (2013) Microb. Biotechnol. 6:17-26, включенные в настоящий документ посредством ссылки.
[0019] Свыше 750 различных последовательностей гена cry были классифицированы по 73 группам (Cry1-Cry73), и открытия новых членов этого семейства генов продолжаются (Crickmore et al. (2014) www.btnomenclature.info/). Семейство генов cry состоит из нескольких филогенетически не связанных белковых семейств, которые могут обладать действием различного типа: семейство трехдоменных Cry-токсинов, семейство воздействующих на комаров Cry-токсинов, семейство бинарных токсинов и Cyt-семейство токсинов (Bravo et al., 2005). Некоторые Bt-штаммы производят дополнительные инсектицидные токсины, VIP-токсины. Смотри, также, документы Cohen et al. (2011) J. Mol. Biol. 413:4-814; Crickmore et al. (2014), номенклатура токсинов Bacillus thuringiensis, расположена во всемирной сети по адресу lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/; Crickmore et al. (1988) Microbiol. Mol. Biol. Rev. 62: 807-813; Gill et al. (1992) Ann. Rev. Entomol. 37: 807-636; Goldbert et al. (1997) Appl. Environ. Microbiol. 63:2716-2712; Knowles et al. (1992) Proc. R. Soc. Ser. B. 248: 1-7; Koni et al. (1994) Microbiology 140: 1869-1880; Lailak et al. (2013) Biochem. Biophys. Res. Commun. 435: 216-221; Lopez-Diaz et al. (2013) Environ. Microbiol. 15: 3030-3039; Perez et al. (2007) Cell. Microbiol. 9: 2931-2937; Promdonkoy et al. (2003) Biochem. J. 374: 255-259; Rigden (2009) FEBS Lett. 583: 1555-1560; Schnepf et al. (1998) Microbiol. Mol. Biol. Rev. 62: 775-806; Soberon et al. (2013) Peptides 41: 87-93; Thiery et al. (1998) J. Am. Mosq. Control Assoc. 14: 472-476; Thomas et al. (1983) FEBS Lett. 154: 362-368; Wirth et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94: 10536-10540; Wirth et al (2005) Appl. Environ. Microbiol. 71: 185-189; и Zhang et al. (2006) Biosci. Biotechnol. Biochem. 70: 2199-2204; которые включены в настоящий документ посредством ссылки во всей полноте.
[0020] Cyt обозначает белок параспорального кристаллического включения из Bacillus thuringiensis с цитолитической активностью или белок со сходством последовательностей с известным Cyt-белком. (Crickmore et al. (1998) Microbiol. Mol. Biol. Rev. 62: 807-813). Ген обозначается cyt. Эти белки отличаются по структуре и активности от Cry-белков (Gill et al. (1992) Annu. Rev. Entomol. 37: 615-636). Cyt-токсины были впервые открыты в B. Thuringiensis, подвид israelensis (Goldberg et al. (1977) Mosq. News. 37: 355-358). Существуют 3 семейства Cyt-токсинов, включающие в себя 11 голотипов токсинов по современной номенклатуре (Crickmore et al. (2014), номенклатура токсинов Bacillus thuringiensis, расположена во всемирной сети по адресу lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/). Большинство изолятов B. thuringiensis с генами cyt демонстрируют активность в отношении двукрылых насекомых (особенно комаров и черных мух), но существуют также гены cyt, которые были описаны в штаммах B. Thuringiensis, воздействующие на чешуекрылых или жесткокрылых насекомых (Guerchicoff et al. (1997) Appl. Environ. Microbiol. 63: 2716-2721).
[0021] Структура Cyt2A, разрешенная с помощью рентгеновской кристаллографии, демонстрирует один домен, в котором два внешних слоя α-спирали обмотаны вокруг смешанного β-листа. Другие доступные кристаллические структуры Cyt-токсинов сохраняют модель консервативной структуры α-β с двумя α-спиральными шпильками, фланкирующими сердцевину β-листа, содержащую от семи до восьми β-цепей. (Cohen et al. (2011) J. Mol. Biol. 413: 80 4-814). Мутагенные исследования идентифицировали остатки β-листа как существенные для токсичности, тогда как мутации в спиральных доменах не влияют на токсичность (Adang et al.; Diversity of Bacillus thuringiensis Crystal Toxins and Mechanism of Action. в T. S. Dhadialla и S. S. Gill, eds, Advances in Insect Physiology, Vol. 47, Oxford: Academic Press, 2014, pp. 39-87.) Типичным доменом Cyt-токсина является δ-эндотоксин, Bac_thur_toxin (Pfam PF01338).
[0022] Предложено несколько моделей способа действия Cyt-токсинов, и их продолжаеют активно изучать. Было показано, что некоторые Cyt-белки (Cyt1A) требуют для кристаллизации присутствия акцессорных белков. Прототоксины Cyt1A и Cyt2A обрабатываются пищеварительными протеазами в одних и тех же участках N- и C-концов до сердцевины стабильного токсина. Затем Cyt-токсины взаимодействуют с ненасыщенными мембранными липидами, такими как фосфатидилхолин, фосфатидилэтаноламин и сфингомиелин. Предполагается, что для Cyt-токсинов неисключительными механизмами являются порообразование и аналогичное детергентам разрушение мембраны; и обычно считают, что они оба могут иметь место в зависимости от концентрации токсина, причем более низкие концентрации способствуют олигомерным порам, а более высокие концентрации приводят к разрушению мембраны. (Butko (2003) Appl. Environ. Microbiol. 69: 2415-2422). В модели порообразования Cyt-токсин связывается с клеточной мембраной, индуцируя образование катион-селективных каналов в мембранных везикулах, что ведет к коллоидно-осмотическому лизису клетки. (Knowles et al. (1989) FEBS Lett. 244: 259-262; Knowles et al. (1992) Proc. R. Soc. Ser. B. 248: 1-7 и Promdonkoy et al. (2003) Biochem. J. 374: 255-259). В модели детергента имеет место неспецифическая агрегация токсина на поверхности липидного бислоя, что ведет к разрушению мембраны и смерти клетки. (Butko (2003) выше; Manceva et al. (2005) Biochem. 44: 589-597).
[0023] Несколько исследований показали синергическое действие Cyt-токсинов и других токсинов B. Thuringiensis, в частности токсинов Cry, Bin и Mtx. Было показано, что этот синергизм преодолевает резистентность насекомого к другому токсину. (Wirth 1997, Wirth 2005, Thiery 1998, Zhang 2006). Синергетический эффект Cyt для Cry-токсинов, как полагают, включает в себя связывание Cyt1A с доменом II Cry-токсинов в растворе или на плоскости мембраны, способствующее образованию предшествующего порам олигомеру Cry-токсинов. Образование этого олигомера не зависит от олигомеризации, связывания или вставки Cyt. (Lailak 2013, Perez 2007, Lopez-Diaz 2013)
[0024] Ряд пестицидных белков, не связанных с Cry-белками, производятся некоторыми штаммами B. thuringiensis и B. cereus во время вегетативного роста (Estruch et al. (1996) Proc Natl Acad Sci USA 93:5389-5394; Warren et al. (1994) WO 94/21795). Эти вегетативные инсектицидные белки, или Vip, не образуют белков параспоральных кристаллов и, по-видимому, секретируются из клетки. Vip в настоящее время исключают из номенклатуры Cry-белков, поскольку они не являются образующими кристаллы белками. Термин VIP является неправильным в том смысле, что некоторые Cry-белки B. thuringiensis также производятся во время вегетативного роста, а также во время стационарной фазы и фазы спорообразования, в первую очередь Cry3Aa. Сообщалось, что локализация генов Vip в геноме B. thuringiensis расположена на больших плазмидах, которые также кодируют гены cry (Mesrati et al. (2005) FEMS Microbiol. Lett. 244(2):353-8). Веб-сайт по номенклатуре Bt-токсинов можно найти во всемирной сети на сайте lifesci.sussex.ac.uk с путем "/home/Neil_Crickmore/Bt/" и на сайте "btnomenclature.info/". Смотри также Schnepf et al. (1998) Microbiol. Mol. Biol. Rev. 62(3):775-806. Такие ссылки включены в настоящий документ посредством ссылки.
[0025] На сегодняшний день определены четыре категории Vip. Некоторые гены Vip образуют бинарные двухкомпонентные белковые комплексы; компонент "A" обычно является "активной" частью, а компонент "B" обычно является "связывающей" частью. (Pfam pfam.xfam.org/family/PF03495). Белки Vip1 и Vip4 обычно содержат домены белка бинарного токсина B. Белки Vip2 обычно содержат домены белка бинарного токсина A.
[0026] Белки Vip1 и Vip2 являются двумя компонентами бинарного токсина, который проявляет токсичность по отношению к жесткокрылым. Vip1Aa1 и Vip2Aa1 очень активны в отношении кукурузных жуков, особенно Diabrotica virgifera и Diabrotica longicornis (Han et al. (1999) Nat. Struct. Biol. 6:932-936; Warren GW (1997) "Vegetative insecticidal proteins: novel proteins for control of corn pests" в Carozzi NB, Koziel M (eds) Advances in insect control, the role of transgenic plants; Taylor & Francis Ltd, London, pp 109-21). Связывающийся с мембраной мультимер Vip1 размером 95 кДа обеспечивает АДФ-рибозилазе Vip2 размером 52 кДа путь для входа в цитоплазму целевых клеток западного кукурузного жука (Warren (1997) выше). NAD-зависимая АДФ-рибозилтрансфераза Vip2, вероятно, модифицирует мономерный актин в Arg177 для блокировки полимеризации, что приводит к потере актинового цитоскелета и, в конечном итоге, к смерти клетки из-за быстрого обмена субъдиниц в актиновых волокнах in vivo (Carlier M. F. (1990) Adv. Biophys. 26:51-73).
[0027] Как и Cry-токсины, активированные токсины Vip3A являются пороформирующими белками, способными делать стабильные ионные каналы в мембране (Lee et al. (2003) Appl. Environ. Microbiol. 69:4648-4657). Белки Vip3 активны в отношении нескольких основных чешуекрылых вредителей (Rang et al. (2005) Appl. Environ. Microbiol. 71(10):6276-6281; Bhalla et al. (2005) FEMS Microbiol. Lett. 243:467-472; Estruch et al. (1998) WO 9844137; Estruch et al. (1996) Proc Natl Acad Sci USA 93:5389-5394; Selvapandiyan et al. (2001) Appl. Environ Microbiol. 67:5855-5858; Yu et al. (1997) Appl. Environ Microbiol. 63:532-536). Vip3A активен в отношении Agrotis ipsilon, Spodoptera frugiperda, Spodoptera exigua, Heliothis virescens и Helicoverpa zea (Warren et al. (1996) WO 96/10083; Estruch et al. (1996) Proc Natl Acad Sci USA 93:5389-5394). Как и Cry-токсины, белки Vip3A должны быть активированы протеазами до распознавания на поверхности эпителия средней кишки специфических мембранных белков, отличных от распознаваемых Cry-токсинами.
[0028] Семейство белковых токсинов MTX характеризуется присутствием консервативного домена ETX_MTX2 (pfam 03318). Члены этого семейства имеют гомологичные последовательности с воздействующими на комаров токсинами Mtx2 и Mtx3 из Bacillus sphaericus, а также с эпсилон-токсином ETX из Clostridiumperfringens (Cole et al. (2004) Nat. Struct. Mol. Biol. 11: 797-8; Thanabalu et al. (1996) Gene 170:85-9). MTX-подобные белки структурно отличаются от трехдоменных Cry-токсинов, так как они имеют удлиненную и преимущественно основанную на β-листах структуру. Однако аналогично трехдоменным токсинам MTX-подобные белки, как полагают, образуют поры в мембранах клеток-мишеней (Adang et al. (2014) выше). В отличие от трехдоменных Cry-белков MTX-подобные белки гораздо меньше по длине и находятся в диапазоне от 267 аминокислот (Cry23) до 340 аминокислот (Cry15A).
[0029] На сегодняшний день только 15 белкам, принадлежащим к семейству подобных MTX токсинов были присвоены названия Cry, что делает этот относительно небольшой класс сравнимым с семейством трехдоменных Cry (Crickmore et al. (2014) выше; Adang et al. (2014)выше). Члены семейства подобных MTX токсинов включают Cry15, Cry23, Cry33, Cry38, Cry45, Cry46, Cry51, Cry60A, Cry60B и Cry64. Это семейство проявляет спектр инсектицидной активности, включая активность в отношении насекомых-вредителей из отрядов чешуекрылых и жесткокрылых. Некоторые члены этого семейства могут формировать парное взаимодействие с другими белками, которое может быть или не быть необходимым для инсектицидной активности.
[0030] Cry15 представляет собой белок размером 34 кДа, который был идентифицирован в Bacillus thuringiensis серовара thompsoni HD542; в природе он встречается в кристалле вместе с неродственным белком размером приблизительно 40 кДа. Гены, кодирующие Cry15 и его белок-партнер, расположены вместе в опероне. Было показано, что сам Cry15 обладает активностью в отношении чешуекрылых насекомых-вредителей, включая Manducasexta, Cydia pomonella и Pieris rapae, а в присутствии белка размером 40 кДа, как было показано, активность Cry15 возрастает только в отношении C. pomonella (Brown K. и Whiteley H. (1992) J. Bacteriol. 174:549-557; Naimov et al. (2008) Appl. Environ. Microbiol. 74:7145-7151). Необходимы дальнейшие исследования для выяснения функции белка-партнера Cry15. Аналогично, Cry23 представляет собой белок размером 29 кДа, который, как показано, обладает активностью в отношении жесткокрылых вредителей Tribolium castaneum и Popillia japonica вместе со своим белком-партнером Cry37 (Donovan et al. (2000) патент США № 6063756).
[0031] Продолжают идентифицировать новые члены MTX-подобного семейства. Ген токсина ETX_MTX недавно был идентифицирован в геноме Bacillus thuringiensis серовара tolworthi, штамм Na205-3. Было обнаружено, что этот штамм токсичен в отношении чешуекрылого вредителя Helicoverpa armigera, и он также содержит гомологи Cry1, Cry11, Vip1, Vip2 и Vip3 (Palma et al. (2014) Genome Announc. 2(2): e00187-14. Опубликовано онлайн 13 марта 2014 г., doi: 10.1128/genomeA.00187-14; PMCID: PMC3953196). Поскольку MTX-подобные белки имеют уникальную доменную структуру относительно трехдоменных Cry-белков, то полагают, что они обладают уникальным способом действия, что делает их ценным инструментом в контроле насекомых и борьбе против резистентности насекомых.
[0032] Бактериальные клетки производят большое количество токсинов с различной специфичностью в отношении организмов-хозяев и не хозяев. В многочисленных семействах бактерий были идентифицированы большие семейства бинарных токсинов, включая токсины, обладающие активностью в отношении насекомых-вредителей. (Poopathi and Abidha (2010) J. Physiol. Path. 1(3): 22-38). Lysinibacillus sphaericus (Ls), ранее Bacillus sphaericus (Ahmed et al. (2007) Int. J. Syst. Evol. Microbiol. 57:1117-1125), хорошо известен как штамм для биоконтроля насекомых. Ls производит несколько инсектицидных белков, включая высокоактивный бинарный комплекс BinA/BinB. Этот бинарный комплекс образует параспоральный кристалл в клетках Ls и обладает сильной и специфической активностью в отношении двукрылых насекомых, особенно комаров. Сообщалось о резистентности насекомых к существующим штаммам Ls, воздействующим на комаров, в некоторых районах. Открытие новых бинарных токсинов с специфичностью к другой мишени или способностью преодолевать резистентность насекомых представляет значительный интерес.
[0033] Бинарный инсектицидный белковый комплекс Ls содержит два основных полипептида, полипептид размером 42 кДа и полипептид размером 51 кДа, обозначаемые BinA и BinB, соответственно (Ahmed et al. (2007) выше). Эти два полипептида действуют синергетически, что придает токсичность по отношению к их мишеням. Способ действия включает в себя связывание белков с рецепторами в средней кишке личинок. В некоторых случаях белки модифицируются посредством расщепления протеазами в кишке личинок для получения активированных форм. Полагают, что компонент BinB участвует в связывания, тогда как компонент BinA придает токсичность (Nielsen-LeRoux et al. (2001) Appl. Environ. Microbiol. 67(11):5049-5054). При отдельном клонировании и экспрессии компонент BinA токсичен для личинок комаров, а компонент BinB нет. При этом совместное введение белков заметно повышает токсичность (Nielsen-LeRoux et al. (2001) выше).
[0034] Было описано небольшое количество гомологов белков Bin из бактериальных источников. Priest et al., (1997) Appl. Environ. Microbiol. 63(4):1195-1198, описывают попытку гибридизации для идентификации новых штаммов Ls, хотя большинство генов, которые они идентифицировали, кодировали белки, идентичные известным белкам BinA/BinB. Белок BinA содержит определенный консервативный домен, известный как домен суперсемейства токсин 10. Этот домен токсина был первоначально определен по его присутствию в BinA и BinB. Оба этих белка имеют этот домен, хотя сходство последовательностей между BinA и BinB ограничено этой областью (<40%). Белок Cry49Aa, который также обладает инсектицидной активностью, также имеет этот домен (описано ниже).
[0035] Бинарный токсин Cry48Aa/Cry49Aa из Ls обладает способностью уничтожать личинок комара Culex quinquefasciatus. Эти белки относятся к структурному классу белков, которые имеют некоторое сходство с комплексом белков Cry из Bacillus thuringiensis (Bt), хорошо известному семейству инсектицидных белков. Также известно, что бинарный токсин Cry34/Cry35 из Bt уничтожает насекомых, включая западного кукурузного жука, значительного вредителя кукурузы. Cry34, у которого было идентифицировано несколько вариантов, представляет собой небольшой (14 кДа) полипептид, тогда как Cry35 (также кодируемый несколькими вариантами) представляет собой полипептид размером 44 кДа. Эти белки имеют некоторую гомологию последовательности с группой белков BinA/BinB и, как полагают, эволюционно связаны (Ellis et al. (2002) Appl. Environ. Microbiol. 68(3):1137-1145).
[0036] В настоящем документе предлагаются пестицидные белки из этих классов токсинов. Пестицидные белки классифицируются по их структуре, гомологии с известными токсинами и/или их пестицидной специфичности.
ii. Варианты и фрагменты пестицидных белков и кодирующие их полинуклеотиды
[0037] Пестицидные белки или полипептиды настоящего изобретения включают приведенные в SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398 и их фрагменты и варианты. Под "пестицидным токсином", или "пестицидным белком", или "пестицидным полипептидом" понимается токсин, или белок, или полипептид, который обладает активностью в отношении одного или нескольких вредителей, включая насекомых, грибы, нематоды и т.п., так что вредитель уничтожается или контрлируется.
[0038] "Выделенный" или "очищенный" полипептид или белок или его биологически активная часть по существу или в основном свободны от компонентов, которые обычно сопровождают или взаимодействуют с полипептидом или белком, находящимися в их природной среде. Таким образом, выделенный или очищенный полипептид или белок по существу свободны от другого клеточного материала или культуральной среды при получении с помощью рекомбинантных методов или по существу свободны от химических предшественников или других химических веществ при химическом синтезе. Белок, который по существу свободен от клеточного материала, включает препараты белка, содержащие менее чем приблизительно 30%, 20%, 10%, 5% или 1% (по сухому весу) загрязняющего белка. Когда белок настоящего изобретения или его биологически активную часть получают рекомбинантно, оптимально, чтобы культуральная среда содержала менее приблизительно 30%, 20%, 10%, 5% или 1% (по сухому весу) химических предшественников или не являющихся представляющим интерес белком химических веществ.
[0039] Термин "фрагмент" относится к части последовательности полипептида настоящего изобретения. "Фрагменты" или "биологически активные части" включают полипептиды, содержащие достаточное число идущих подряд аминокислотных остатков для сохранения биологической активности, т.е. обладающие пестицидной активностью. Фрагменты пестицидных белков короче, чем полноразмерные последовательности, или вследствие использования альтернативного нижележащего сайта начала, или вследствие процессинга, который производит более короткий белок, обладающий пестицидной активностью. Процессинг может происходить в организме, в котором белок экспрессируется, или во вредителе после попадания белка внутрь. Примеры фрагментов белков можно найти в таблице 1. Биологически активная часть пестицидного белка может представлять собой полипептид, который имеет длину, например, 10, 25, 50, 100, 150, 200, 250 или более аминокислот в соответствии с любой из SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398. Такие биологически активные части могут быть получены с помощью рекомбинантных методов и оценены в отношении пестицидной активности. Как используется в настоящем документе, фрагмент содержит по меньшей мере 8 соседних аминокислот в соответствии с SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398.
[0040] Бактериальные гены, включая кодирующие пестицидные белки, раскрытые в настоящем документе, достаточно часто имеют несколько инициаторных кодонов метионина поблизости от начала открытой рамки считывания. Часто инициация трансляции в одном или нескольких из этих стартовых кодонов приводит к генерации функционального белка. Эти стартовые кодоны могут включать в себя кодоны ATG. Однако бактерии, такие как Bacillus sp., также распознают в качестве стартового кодона кодон GTG, и белки, которые инициируют трансляцию на кодонах GTG, содержат метионин как первую аминокислоту. В редких случаях трансляция в бактериальных системах может инициироваться на кодоне TTG, хотя в этом случае TTG кодирует метионин. Кроме того, не часто определено a priori, какие из этих кодонов используются в бактериях в природе. Таким образом, следует понимать, что использование одного из альтернативных кодонов метионина может также приводить к генерации пестицидных белков. Эти пестицидные белки охватываются настоящим изобретением и могут быть использованы в способах, раскрытых в настоящем документе. Следует понимать, что при экспрессии в растениях для правильной трансляции необходимо менять альтернативный стартовый кодон на ATG.
[0041] В различных вариантах осуществления пестицидные белки, предлагаемые в настоящем документе, включают в себя аминокислотные последовательности, выведенные из полноразмерных нуклеотидных последовательностей, и аминокислотные последовательности, которые короче, чем полноразмерные последовательности вследствие использования альтернативного нижележащего сайта начала. Таким образом, нуклеотидная последовательность настоящего изобретения и/или векторы, клетки-хозяева и растения, содержащие нуклеотидную последовательность настоящего изобретения (и способы получения и применения нуклеотидной последовательности настоящего изобретения) могут содержать нуклеотидную последовательность, кодирующую альтернативный сайт начала.
[0042] Следует понимать, что в пестицидных полипептидах, предлагаемых в настоящем документе, могут быть осуществлены модификации, создающие варианты белков. Изменения, разработанные человеком, могут быть введены путем применения методов сайт-направленного мутагенеза. В качестве альтернативы, могут также быть идентифицированы нативные еще неизвестные или еще не идентифицированные полинуклеотиды и/или полипептиды, структурно и/или функционально связанные с последовательностями, раскрытыми в настоящем документе, которые подпадают под объем настоящего изобретения. Могут быть осуществлены консервативные аминокислотные замены в неконсервативных областях, которые не изменяют функцию пестицидных белков. В качестве альтернативы, могут быть осуществлены модификации, которые улучшают активность токсина. Модификация Cry-токсинов посредством замены домена III в некоторых случаях приводит к гибридным токсинам с улучшенной токсичностью в отношении некоторых видов насекомых. Таким образом, замена домена III может быть эффективной стратегией улучшения токсичности Cry-токсинов или создания новых гибридных токсинов с токсичностью в отношении вредителей, которые демонстрируют отсутствие восприимчивости к родительским Cry-токсинам. Сайт-направленный мутагенез последовательностей петли домена II может приводить к новым токсинам с повышенной инсектицидной активностью. Области петли домена II являются ключевыми связывающими областями исходных Cry-токсинов, которые являются подходящими мишенями для мутагенеза и селекции Cry-токсинов с улучшенными инсектицидными свойствами. Домен I Cry-токсина может быть модифицирован для введения сайтов расщепления протеаз для улучшения активности в отношении некоторых вредителей. Стратегии перетасовки трех различных доменов среди большого числа генов cry и способы высокопроизводительного скрининга биологических проб могут предоставить новые Cry-токсины с улучшенной или новой токсичностью.
[0043] Как было указано, фрагменты и варианты полипептидов, раскрытые в настоящем документе, сохраняют пестицидную активность. Пестицидная активность подразумевает способность композиции к достижению наблюдаемого эффекта уменьшения появления или активности целевого вредителя, включая например, вызывание гибели по меньшей мере одного вредителя или заметное снижение роста, питания или нормального физиологического развития вредителей. Такое снижение количества, роста, питания или нормального развития вредителей может включать в себя любое статистически значимое снижение, включая, например, снижение приблизительно на 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 85%, 90%, 95% или больше. Понятно, что пестицидная активность может иной или улучшенной относительно активности нативного белка, или она может быть неизменной, при условии, что пестицидная активность сохраняется. Способы измерения пестицидной активности хорошо известны в данной области техники. Смотри, например, Czapla и Lang (1990) J. Econ. Entomol. 83:2480-2485; Andrews et al. (1988) Biochem. J. 252:199-206; Marrone et al. (1985) J. of Economic Entomology 78:290-293; и патент США № 5743477, которые включены в настоящий документ посредством ссылки во всей полноте.
[0044] Варианты полипептидов настоящего раскрытия включают полипептиды, обладающие аминокислотной последовательностью, которая по меньшей мере приблизительно на 60%, приблизительно на 65%, приблизительно на 70%, приблизительно на 75%, приблизительно на 80%, приблизительно на 85%, приблизительно на 86%, приблизительно на 87%, приблизительно на 88%, приблизительно на 89%, приблизительно на 90%, приблизительно на 91%, приблизительно на 92%, приблизительно на 93%, приблизительно на 94%, приблизительно на 95%, приблизительно на 96%, приблизительно на 97%, приблизительно на 98% или приблизительно на 99% идентична аминокислотной последовательность в соответствии с любой из SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398, и которые сохраняют пестицидную активность. Следует отметить, что таблица 1 предлагает неограничивающие примеры вариантов полипептидов (и кодирующих их полинуклеотидов) для каждой из SEQ ID NO: 1-398. Биологически активный вариант пестицидного полипептида настоящего изобретения может отличаться всего лишь приблизительно на 1-15 аминокислотных остатков, всего лишь приблизительно на 1-10, например приблизительно на 6-10, всего лишь на 5, всего лишь на 4, всего лишь на 3, всего лишь на 2, или всего лишь на 1 аминокислотный остаток. В конкретных вариантах осуществления полипептиды могут иметь N'-концевое или C'-концевое усечение, которое может включать в себя по меньшей мере делецию 10, 15, 20, 25, 30, 35, 40, 45, 50 или более аминокислот или с N'-, или с C'-концевой области полипептида.
[0045] В таблице 2 представлены белковые домены, найденные в SEQ ID NO: 1-398 на основании данных PFAM. В таблице 2 представлены как описание домена, так и положения в указанных SEQ ID NO. В конкретных вариантах осуществления активный вариант, содержащий любую из SEQ ID NO: 1-398, может иметь идентичность последовательности по меньшей мере 70%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% или 99% с любой из SEQ ID NO: 1-398 и дополнительно содержит по меньшей мере один из консервативных доменов, приведенных в таблице 2. Например, в одном варианте осуществления активный вариант имеет идентичность последовательности по меньшей мере 70%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% или 99% с SEQ ID NO: 1 и дополнительно содержит нативные аминокислоты в положениях 54-304.
Таблица 2. Сводная информация о доменах PFAM в каждой из SEQ ID NO: 1-398
[0046] Также предлагаются рекомбинантные или синтетические нуклеиновые кислоты, кодирующие пестицидные полипептиды, раскрытые в настоящем документе. Особый интерес представляют последовательности нуклеиновой кислоты, которые сконструированы для экспрессии в представляющем интерес растении. То есть последовательность нуклеиновой кислоты может быть оптимизирована для увеличения экспрессии в растении-хозяине. Пестицидный белок настоящего изобретения может быть подвергнут обратной трансляции для получения нуклеиновой кислоты, содержащей кодоны, оптимизированные для экспрессии в конкретном хозяине, например культурном растении. В другом варианте осуществления полинуклеотиды, кодирующие полипептиды, предлагаемые в настоящем документе, могут быть оптимизированы для увеличения экспрессии в трансформированном растении. То есть полинуклеотиды могут быть синтезированы с использованием предпочтительных для растения кодонов для улучшения экспрессии. Смотри, например, Campbell and Gowri (1990) Plant Physiol. 92:1-11, для рассмотрения использования предпочтительных для хозяина кодонов. В данной области техники доступны способы синтеза предпочтительных для растения генов. Смотри, например, патенты США №№ 5380831 и 5436391 и Murray et al. (1989) Nucleic Acids Res. 17:477-498, включенные в настоящий документ посредством ссылки. Экспрессия такой кодирующей последовательности трансформированным растением (например, двудольным или однодольным) будет приводить к получению пестицидного полипептида и придаст растению повышенную резистентность к вредителю. Рекомбинантные и синтетические молекулы нуклеиновой кислоты, кодирующие пестицидные белки настоящего изобретения, не включают в себя природную бактериальную последовательность, кодирующую такой белок.
[0047] "Рекомбинантный полинуклеотид" или "рекомбинантная нуклеиновая кислота" содержат комбинацию двух или более химически связанных сегментов нуклеиновой кислоты, которые не обнаружены непосредственно соединенными в природе. Под "непосредственным соединением" понимается, что два сегмента нуклеиновой кислоты расположены в непосредственной близости и соединены друг с другом с помощью химической связи. В конкретных вариантах осуществления рекомбинантный полинуклеотид содержит такой представляющий интерес полинуклеотид или его вариант или фрагмент, что с 5'-, 3'-конца или внутри представляющего интерес полинуклеотида расположен дополнительный химически связанный сегмент нуклеиновой кислоты. В качестве альтернативы, химически связанный сегмент нуклеиновой кислоты рекомбинантного полинуклеотида может быть образован делецией последовательности. Дополнительный химически связанный сегмент нуклеиновой кислоты или последовательность, удаленная для соединения связанных сегментов нуклеиновой кислоты, могут иметь любую длину, включая например, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20 или больше нуклеотидов. Различные способы получения таких рекомбинантных полинуклеотидов включают химический синтез или манипуляции с изолированными сегментами полинуклеотидов с помощью методов генной инженерии. В конкретных вариантах осуществления рекомбинантный полинуклеотид может содержать рекомбинантную последовательность ДНК или рекомбинантную последовательность РНК. "Фрагмент рекомбинантного полинуклеотида или нуклеиновой кислоты" содержит по меньшей мере одну комбинацию двух или более химически связанных аминокислотных сегментов, которые не обнаружены непосредственно соединенными в природе.
[0048] Фрагменты полинуклеотида (РНК или ДНК) могут кодировать фрагменты белка, которые сохраняют активность. В конкретных вариантах осуществления фрагмент рекомбинантного полинуклеотида или конструкция рекомбинантного полинуклеотида содержат по меньшей мере одно соединение двух или более химически связанных или функционально связанных сегментов нуклеиновой кислоты, которые не обнаружены непосредственно соединенными в природе. Фрагмент полинуклеотида, который кодирует биологически активную часть полипептида, который сохраняет пестицидную активность, будет кодировать по меньшей мере 25, 30, 40, 50, 60, 70, 75, 80, 90, 100, 110, 120, 125, 130, 140, 150, 160, 170, 175, 180 соседних аминокислот, или вплоть до полного числа аминокислот, присутствующих в полноразмерном полипептиде, как приведено в SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398. В конкретных вариантах осуществления такие фрагменты полипептида являются активными фрагментами, а в других вариантах осуществления фрагмент полипептида содержит рекомбинантный фрагмент полипептида. Как используется в настоящем документе, фрагмент рекомбинантного полипептида содержит по меньшей мере одну комбинацию двух или более химически связанных аминокислотных сегментов, которые не обнаружены непосредственно соединенными в природе.
[0049] Под "вариантами" понимаются по существу аналогичные последовательности. Для полинуклеотидов вариант содержит делецию и/или добавление одного или нескольких нуклеотидов в одном или нескольких внутренних сайтах в нативном полинуклеотиде и/или замену одного или нескольких нуклеотидов в одном или нескольких сайтах в нативном полинуклеотиде. Как используется в настоящем документе, "нативный" полинуклеотид или полипептид содержит природную нуклеотидную последовательность или аминокислотную последовательность, соответственно.
[0050] Варианты конкретного полинуклеотида настоящего изобретения (т.е. исходного полинуклеотида) можно также оценивать путем сравнения процентной идентичности последовательности между полипептидом, кодируемым вариантом полинуклеотида, и полипептидом, кодируемым исходным полинуклеотидом. Таким образом, например, раскрыты выделенные полинуклеотиды, которые кодируют полипептиды с заданной процентной идентичностью последовательности с полипептидом в соответствии с SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398. Процентная идентичность последовательности между любыми двумя полипептидами может быть вычислена с использованием программ выравнивания последовательностей и параметров, описанных в настоящем документе в другом месте. Если любую заданную пару полинуклеотидов настоящего изобретения оценивают посредством сравнения процентной идентичности последовательности между двумя полипептидами, которые они кодируют, процентная идентичность последовательности между двумя кодируемыми полипептидами представляет собой идентичность последовательности по меньшей мере приблизительно 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% или более с SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398. В других вариантах осуществления вариант полинуклеотида, предлагаемый в настоящем документе, отличается от нативной последовательности по меньшей мере на 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 или больше нуклеотидов.
[0051] Варианты полинуклеотида и белков также охватывают последовательности и белки, получаемые с помощью мутагенной и рекомбиногенной процедуры, такой как перетасовка ДНК. С помощью такой процедуры один или несколько различных пестицидных белков, раскрытых в настоящем документе (SEQ ID NO: 1-209), обрабатывают для создания нового пестицидного белка, имеющего желаемые свойства. Таким образом генерируют библиотеки рекомбинантных полинуклеотидов из популяции полинуклеотидов с родственными последовательностями, содержащие области последовательности, которые обладают существенной идентичностью последовательности и могут быть подвергнуты гомологичной рекомбинации in vitro или in vivo. Например, при использовании этого подхода мотивы последовательностей, кодирующие представляющий интерес домен, могут быть перетасованы между пестицидными последовательностями, предлагаемыми в настоящем документе, и другими известными пестицидными генами для получения нового гена, кодирующего белок с улучшенным представляющим интерес свойством, например, увеличенной Km в случае фермента. Стратегии такой перетасовки ДНК известны в данной области техники. Смотри, например, Stemmer (1994) Proc. Natl. Acad. Sci. USA 91:10747-10751; Stemmer (1994) Nature 370:389-391; Crameri et al. (1997) Nature Biotech. 15:436-438; Moore et al. (1997) J. Mol. Biol. 272:336-347; Zhang et al.(1997) Proc. Natl. Acad. Sci. USA 94:4504-4509; Crameri et al. (1998) Nature 391:288-291; и патенты США №№ 5605793 и 5837458. "Перетасованная" нуклеиновая кислота представляет собой нуклеиновую кислоту, полученную с помощью процедуры перетасовки, такой как любая процедура перетасовки, приведенная в настоящем документе. Перетасованные нуклеиновые кислоты получают посредством рекомбинации (физической или виртуальной) двух или более нуклеиновых кислот (или цепочек символов), например искусственным и, необязательно, рекурсивным образом. Обычно в процессах перетасовки используют один или несколько этапов скрининга для идентификации представляющих интерес нуклеиновых кислот; этот этап скрининга может быть осуществлен до или после любого этапа рекомбинации. В некоторых (но не во всех) вариантах осуществления перетасовки желательно осуществление нескольких циклов рекомбинации до селекции для увеличения разнообразия подлежащего скринингу пула. Весь процесс рекомбинации и селекции, необязательно, повторяют рекурсивно. В зависимости от контекста, перетасовка может относиться ко всему процессу рекомбинации и селекции или, альтернативно, может относиться просто к рекомбинационным частям всего процесса.
[0052] В одном варианте осуществления предлагается способ получения полинуклеотида, который кодирует улучшенный полипептид, имеющий пестицидную активность, причем улучшенный полипептид обладает по меньшей мере одним улучшенным свойством для любой из SEQ ID NO: 1-229. Такие способы могут содержать (a) рекомбинацию множества родительских полинуклеотидов для получения библиотеки рекомбинантных полинуклеотидов, кодирующих рекомбинантные пестицидные полипептиды; (b) скрининг библиотеки для идентификации рекомбинантного полинуклеотида, который кодирует улучшенный рекомбинантный пестицидный полипептид, который обладает усиленным свойством, улучшенным по сравнению с родительским полинуклеотидом; (c) восстановление рекомбинантного полинуклеотида, который кодирует улучшенный рекомбинантный пестицидный полипептид, идентифицированный в (b); и (d) повторение этапов (a), (b) и (c) с использованием рекомбинантного полинуклеотида, восстановленного на этапе (c), в качестве одного из множества родительских полинуклеотидов при повторении этапа (a).
iii. Сравнения последовательностей
[0053] Как используется в настоящем документе, термин "идентичность" или "процентная идентичность" при использовании по отношению к конкретной паре выровненных аминокислотных последовательностей относится к процентной идентичности аминокислотной последовательности, которую получают посредством подсчета числа идентичных совпадений в выравнивании и деления такого числа идентичных совпадений на длину выровненных последовательностей. Как используется в настоящем документе, термин "сходство" или "процентное сходство" при использовании по отношению к конкретной паре выровненных аминокислотных последовательностей относится к сумме баллов, которые получают из оценочной матрицы для каждой пары аминокислот в выравнивании, деленной на длину выровненных последовательностей.
[0054] Если не указано иное, идентичность и сходство будут вычисляться посредством алгоритмов глобального выравнивания и оценки Нидлмана-Вунша (Needleman and Wunsch (1970) J. Mol. Biol. 48(3):443-453), реализованных в программе "needle", распространяемой как часть пакета программного обеспечения EMBOSS (Rice,P. Longden,I. and Bleasby,A., EMBOSS: The European Molecular Biology Open Software Suite, 2000, Trends in Genetics 16, (6) pp276-277, версия 6.3.1, доступно от EMBnet по адресу embnet.org/resource/emboss и emboss.sourceforge.net, а также в других источниках) с использованием стандартных штрафов за гэп и оценочных матриц (EBLOSUM62 для белка и EDNAFULL для ДНК). Можно также использовать эквивалентные программы. Под "эквивалентной программой" понимается любая программа для сравнения последовательностей, которая для любых двух рассматриваемых последовательностей генерирует выравнивание, имеющее идентичные совпадения нуклеотидных остатков и идентичную процентную идентичность последовательности при сравнении с соответствующим выравниванием, сгенерированным программой needle от EMBOSS версии 6.3.1.
[0055] Дополнительные математические алгоритмы известны в данной области техники и могут быть использованы для сравнения двух последовательностей. Смотри, например, алгоритм в документе Karlin and Altschul (1990) Proc. Natl. Acad. Sci. USA 87:2264, модифицированный как в документе Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA 90:5873-5877. Такой алгоритм включен в программы BLAST из документа Altschul et al. (1990) J. Mol. Biol. 215:403. Поиск нуклеотидов BLAST может осуществляться с помощью программы BLASTN (поиск запроса из нуклеотидов в нуклеотидных последовательностях) для получения нуклеотидных последовательностей, гомологичных подобным пестицидам молекул нуклеиновой кислоты настоящего изобретения, или с помощью программы BLASTX (поиск запроса из транслированных нуклеотидов в белковых последовательностях) для получения белковых последовательностей, гомологичных пестицидным молекулам нуклеиновой кислоты настоящего изобретения. Поиск белков BLAST может быть осуществлен с помощью программы BLASTP (поиск запроса из белков в белковых последовательностях) для получения аминокислотных последовательностей, гомологичных пестицидным белковым молекулам настоящего изобретения, или с помощью программы TBLASTN (поиск запроса из белков в транслированных нуклеотидных последовательностях) для получения нуклеотидных последовательностей, гомологичных пестицидным белковым молекулам настоящего изобретения. Для получения с целью сравнения выравниваний с разрывами можно использовать Gapped BLAST (в BLAST 2.0), как описано в документе Altschul et al. (1997) Nucleic Acids Res. 25:3389. В качестве альтернативы можно использовать PSI-Blast для осуществления итерационного поиска, который детектирует отдаленные связи между молекулами. Смотри Altschul et al. (1997) выше. При использовании программ BLAST, Gapped BLAST и PSI-Blast можно использовать стандартные параметры соответствующих программ (например, BLASTX и BLASTN). Выравнивание также можно выполнять вручную с помощью проверки.
[0056] Две последовательности "оптимально выровнены", если они выровнены по баллам сходства с использованием определенной матрицы аминокислотных замен (например, BLOSUM62), штрафа за открытие гэпа и штрафа за продолжение гэпа таким образом, что достигнут наивысший возможный балл для данной пары последовательностей. Матрицы аминокислотных замен и их применение в количественной оценке сходства между двумя последовательностями хорошо известны в данной области техники и описаны, например, в документах Dayhoff et al. (1978) "A model of evolutionary change in proteins" в "Atlas of Protein Sequence and Structure", Vol. 5, Suppl. 3 (ed. M. O. Dayhoff), pp. 345-352. Natl. Biomed. Res. Found., Washington, D.C., и Henikoff et al. (1992) Proc. Natl. Acad. Sci. USA 89:10915-10919. В качестве стандартной оценочной матрицы замен в протоколах выравнивания последовательностей часто используют матрицу BLOSUM62. Штраф за открытие гэпа накладывается за введение гэпа из одной аминокислоты в одной из выровненных последовательностей, а штраф за продолжение гэпа накладывается за каждое дополнительное пустое аминокислотное положение, вставленное в уже открытый гэп. Выравнивание определяют по аминокислотным положениям каждой последовательности, в которых выравнивание начинается и заканчивается, и, необязательно, по вставке гэпа или нескольких гэпов в одну или обе последовательности, таким образом, что достигнут наивысший возможный балл. При том, что оптимальные выравнивание и оценка могут быть получены вручную, данный процесс облегчается за счет использования реализуемого компьютером алгоритма выравнивания, например gapped BLAST 2.0, описанного в документе Altschul et al. (1997) Nucleic Acids Res. 25:3389-3402 и общедоступного на веб-сайте Национального центра биотехнологической информации (www.ncbi.nlm.nih.gov). Оптимальные выравнивания, включая множественные выравнивания, могут быть получены с использованием, например, PSI-BLAST, доступного на www.ncbi.nlm.nih.gov и описанного в документе Altschul et al. (1997) Nucleic Acids Res. 25:3389-3402.
[0057] По отношению к аминокислотной последовательности, которая оптимально выровнена с эталонной последовательностью, аминокислотный остаток "соответствует" положению в эталонной последовательности, которому данный остаток при выравнивании является парным. "Положение" обозначают номером, который последовательно идентифицирует каждую аминокислоту в эталонной последовательности на основании ее положения относительно N-конца. Например, в SEQ ID NO: 177 положением 1 является M, положением 2 является N, положением 3 является E и т.д. Когда тестовая последовательность оптимально выровнена с SEQ ID NO: 177, остаток в тестовой последовательности, который выровнен с E в положении 3, называют "соответствующим положению 3" в SEQ ID NO: 177. Благодаря делециям, вставкам, усечениям, слияниям и т.д., которые следует принимать во внимание при определении оптимального выравнивания, номер аминокислотного остатка в тестовой последовательности, определенный простым отсчетом от N-конца, в общем случае не обязательно совпадает с номером соответствующего ему положения в эталонной последовательности. Например, в случае, когда в выровненной тестовой последовательности есть делеция, аминокислота, которая соответствует положению в эталонной последовательности в сайте делеции, будет отсутствовать. Если в выровненной эталонной последовательности есть вставка, эта вставка не будет соответствовать никакому аминокислотному положению в эталонной последовательности. В случае усечений или слияний могут существовать интервалы аминокислот или в эталонной, или в выровненной последовательности, которые не соответствуют никакой аминокислоте в соответствующей последовательности.
iv. Антитела
[0058] Также охвачены антитела к полипептидам настоящего изобретения или к их вариантам или фрагментам. Способы получения антител хорошо известны в данной области техники (смотри, например, Harlow и Lane (1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.; и патент США № 4196265). Эти антитела можно использовать в наборах для детектирования и выделения полипептидов токсинов. Таким образом, настоящее раскрытие предлагает наборы, содержащие антитела, которые специфически связываются с полипептидами, описанными в настоящем документе, включая, например, полипептиды, имеющие последовательность в соответствии с SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398.
II. Вредители
[0059] Композиции и способы, предлагаемые в настоящем документе, применяются против множества вредителей. "Вредители" включают, но без ограничения, насекомые, грибы, бактерии, нематоды, акариды, протозойные патогены, паразитирующие на животных печеночные двуустки и т.п. Представляющими особый интерес вредителями являются насекомые-вредители, особенно насекомые-вредители, которые вызывают значительное повреждение сельскохозяйственных растений. Насекомые-вредители включают насекомых, выбранных из отрядов Coleoptera, Diptera, Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera, Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera, или нематод. В неограничивающих вариантах осуществления к насекомым-вредителям относятся жук западный кукурузный, Diabrotica virgifera virgifera; совка травяная, Spodoptera frugiperda; жук колорадский картофельный, Leptinotarsa decemlineata; совка кукурузная, Helicoverpa zea (в Северной Америке этот вид поражает хлопок и называется коробочным червем); мотылек кукурузный, Ostrinia nubilalis; совка-ипсилон, Agrotis ipsilon; моль капустная, Plutella xylostella; совка бархатных бобов, Anticarsia gemmatalis; огневка кукурузная юго-западная, Diatraea grandiosella; коробочный червь, Helicoverpa armigera (обнаруживаемый в остальном мире, кроме США); щитник зеленый южный, Nezara viridula; щитник зеленый, Chinavia halaris; щитник коричневый мраморный, Halyomorpha halys; и щитник коричневый, Euschistus servus, Euschistus heros (неотропический коричневый щитник или соевый щитник); Piezodorus guildinii (краснополосый щитник); Dichelops melacanthus (нет обычного названия) и/или Dichelops furcatus (нет обычного названия); тля, такая как соевая тля. В других вариантах осуществления вредитель включает нематоду, включая, но без ограничения, Meloidogyne hapla (северная галловая нематода); Meloidogyne enterolobii, Meloidogyne arenaria (арахисовая галловая нематода); и Meloidogyne javanica.
[0060] Термин "насекомые-вредители", как используется в настоящем документе, относится к насекомым и другим аналогичным вредителям, таким как, например, из отряда Acari, включая, но без ограничения, клещей и иксодовых клещей. Насекомые-вредители настоящего изобретения включают, но без ограничения, насекомых из отряда Lepidoptera, например Achoroia grisella, Acleris gloverana, Acleris variana, Adoxophyes orana, Agrotis ipsilon, Alabama argillacea, Alsophila pometaria, Amyelois transitella, Anagasta kuehniella, Anarsia lineatella, Anisota senatoria, Antheraea pernyi, Anticarsia gemmatalis, Archips sp., Argyrotaenia sp., Athetis mindara, Bombyx mori, Bucculatrix thurberiella, Cadra cautella, Choristoneura sp., Cochylls hospes, Colias eurytheme, Corcyra cephalonica, Cydia latiferreanus, Cydia pomonella, Datana integerrima, Dendrolimus sibericus, Desmiafeneralis, Diaphania hyalinata, Diaphania nitidalis, Diatraea grandiosella, Diatraea saccharalis, Ennomos subsignaria, Eoreuma loftini, Esphestia elutella, Erannis tilaria, Estigmene acrea, Eulia salubricola, Eupocoellia ambiguella, Eupoecilia ambiguella, Euproctis chrysorrhoea, Euxoa messoria, Galleria mellonella, Grapholita molesta, Harrisina americana, Helicoverpa subflexa, Helicoverpa zea, Heliothis virescens, Hemileuca oliviae, Homoeosoma electellum, Hyphantia cunea, Keiferia lycopersicella, Lambdina fiscellaria fiscellaria, Lambdina fiscellaria lugubrosa, Leucoma salicis, Lobesia botrana, Loxostege sticticalis, Lymantria dispar, Macalla thyrisalis, Malacosoma sp., Mamestra brassicae, Mamestra configurata, Manduca quinquemaculata, Manduca sexta, Maruca testulalis, Melanchra picta, Operophtera brumata, Orgyia sp., Ostrinia nubilalis, Paleacrita vernata, Papilio cresphontes, Pectinophora gossypiella, Phryganidia californica, Phyllonorycter blancardella, Pieris napi, Pieris rapae, Plathypena scabra, Platynota flouendana, Platynota stultana, Platyptilia carduidactyla, Plodia interpunctella, Plutella xylostella, Pontia protodice, Pseudaletia unipuncta, Pseudoplasia includens, Sabulodes aegrotata, Schizura concinna, Sitotroga cerealella, Spilonta ocellana, Spodoptera sp., Thaurnstopoea pityocampa, Tinsola bisselliella, Trichoplusia hi, Udea rubigalis, Xylomyges curiails и Yponomeuta padella.
[0061] Насекомые-вредители также включают насекомых, выбранных из отрядов Diptera, Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera, Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera, Coleoptera.
[0062] В число насекомых-вредителей настоящего изобретения для основных сельскохозяйственных культур входят, но без ограничения: для кукурузы: Ostrinia nubilalis, мотылек кукурузный; Agrotis ipsilon, совка-ипсилон; Helicoverpa zeae, совка кукурузная; Spodoptera frugiperda, совка травяная; Diatraea grandiosella, огневка кукурузная юго-западная; Elasmopalpus lignosellus, огневка кукурузная малая; Diatraea saccharalis, огневка сахарного тростника; жук кукурузный западный, например Diabrotica virgifera virgifera; жук кукурузный северный, например Diabrotica longicornis barberi; жук кукурузный южный, например Diabrotica undecimpunctata howardi; Melanotus spp., проволочники; Cyclocephala borealis, северный хрущ (личинка хруща); Cyclocephala immaculata, южный хрущ (личинка хруща); Popillia japonica, хрущик японский; Chaetocnema pulicaria, блошка кукурузная; Sphenophorus maidis, долгоносик кукурузный; Rhopalosiphum maidis, тля кукурузная листовая; Anuraphis maidiradicis, тля кукурузная корневая; Blissus leucopterus leucopterus, клоп-черепашка; Melanoplus femurrubrum, кобылка краснобедрая; Melanoplus sanguinipes, кобылка перелетная; Hylemya platura, личинка мухи ростковой; Agromyza parvicornis, минер кукурузный листовой; Anaphothrips obscrurus, трипс злаковый; Solenopsis milesta, муравей-вор; Tetranychus urticae, клещ паутинный двупятнистый; для сорго: Chilo partellus, огневка сорговая; Spodoptera frugiperda, совка травяная; Helicoverpa zea, совка кукурузная; Elasmopalpus lignosellus, огневка кукурузная малая; Feltia subterranea, совка хлопковая; Phyllophaga crinita, личинка хруща; Eleodes, Conoderus и Aeolus spp., проволочники; Oulema melanopus, пьявица красногрудая; Chaetocnema pulicaria, блошка кукурузная; Sphenophorus maidis, долгоносик кукурузный; Rhopalosiphum maidis; тля кукурузная листовая; Sipha flava, тля сахарного тростника желтая; клоп-черепашка, например Blissus leucopterus leucopterus; Contarinia sorghicola, галлица сорговая; Tetranychus cinnabarinus, клещ паутинный красный; Tetranychus urticae, клещ паутинный двупятнистый; для пшеницы: Pseudaletia unipunctata, совка луговая; Spodoptera frugiperda, совка травяная; Elasmopalpus lignosellus, огневка кукурузная малая; Agrotis orthogonia, совка прямоугольная; Elasmopalpus lignosellus, огневка кукурузная малая; Oulema melanopus, пьявица красногрудая; Hypera punctata, долгоносик листовой бобовый; жук кукурузный южный, например Diabrotica undecimpunctata howardi; тля русская пшеничная; Schizaphis graminum, тля злаковая; Macrosiphum avenae, тля злаковая большая; Melanoplus femurrubrum, кобылка краснобедрая; Melanoplus differentialis, кобылка отличительная; Melanoplus sanguinipes, кобылка перелетная; Mayetiola destructor, мушка гессенская; Sitodiplosis mosellana, галлица злаковая оранжевая; Meromyza americana, личинка меромизы американской; Hylemya coarctata, муха озимая; Frankliniella fusca, трипс табачный; Cephus cinctus, пилильщик хлебный; Aceria tulipae, луковичный клещ тюльпанов; для подсолнечника: Cylindrocupturus adspersus, долгоносик подсолнечниковый стеблевой; Smicronyx fulus, долгоносик подсолнечниковый красный; Smicronyx sordidus, долгоносик подсолнечниковый серый; Suleima helianthana, листовертка подсолнечниковая; Homoeosoma electellum, метлица подсолнечниковая; Zygogramma exclamationis, совка подсолнечниковая восклицательная; Bothyrus gibbosus, жук морковный; Neolasioptera murtfeldtiana, галлица подсолнечниковая; для хлопка: Heliothis virescens, листовертка табачная; Helicoverpa zea, коробочный червь; Spodoptera exigua, совка малая; Pectinophora gossypiella, розовый коробочный червь; долгоносик хлопковый, например Anthonomus grandis; Aphis gossypii, тля хлопковая; Pseudatomoscelis seriatus, слепняк хлопковый; Trialeurodes abutilonea, белокрылка окаймленная; Lygus lineolaris, клоп травяной; Melanoplus femurrubrum, кобылка краснобедрая; Melanoplus differentialis, кобылка отличительная; Thrips tabaci, трипс луковый; Franklinkiella fusca, трипс табачный; Tetranychus cinnabarinus, клещ паутинный красный; Tetranychus urticae, клещ паутинный двупятнистый; для риса: Diatraea saccharalis, точильщик сахарного тростника; Spodoptera frugiperda, совка травяная; Helicoverpa zea, совка кукурузная; Colaspis brunnea, коласпис виноградный; Lissorhoptrus oryzophilus, долгоносик рисовый водяной; Sitophilus oryzae, долгоносик рисовый; Nephotettix nigropictus, цикадка рисовая; клоп-черепашка, например Blissus leucopterus leucopterus; Acrosternum hilare, щитник зеленый; для сои: Pseudoplusia includens, сока соевая; Anticarsia gemmatalis, совка бархатных бобов; Plathypena scabra, совка клеверная; Ostrinia nubilalis, мотылек кукурузный; Agrotis ipsilon, совка-ипсилон; Spodoptera exigua, совка малая; Heliothis virescens, листовертка табачная; Helicoverpa zea, коробочный червь; Epilachna varivestis, зерновка бобовая мексиканская; Myzus persicae, тля персиковая зеленая; Empoasca fabae, цикадка картофельная; Acrosternum hilare, щитник зеленый; Melanoplus femurrubrum, кобылка краснобедрая; Melanoplus differentialis, кобылка отличительная; Hylemya platura, личинка мухи ростковой; Sericothrips variabilis, трипс соевый; Thrips tabaci, трипс луковый; Tetranychus turkestani, клещ паутинный атлантический; Tetranychus urticae, клещ паутинный двупятнистый; для ячменя: Ostrinia nubilalis, мотылек кукурузный; Agrotis ipsilon, совка-ипсилон; Schizaphis graminum, тля злаковая; клоп-черепашка, например Blissus leucopterus leucopterus; Acrosternum hilare, щитник зеленый; Euschistus servus, щитник коричневый; Jylemya platura, личинка мухи ростковой; Mayetiola destructor, мушка гессенская; Petrobia latens, бурый хлебный клещ; для рапса масличного: Vrevicoryne brassicae, тля капустная; Phyllotreta cruciferae, блошка крестоцветная; Phyllotreta striolata, блошка полосатая; Phyllotreta nemorum, блошка светлоногая полосатая; Meligethes aeneus, цветоед рапсовый; и блестянки Meligethes rufimanus, Meligethes nigrescens, Meligethes canadianus и Meligethes viridescens; для картофеля: Leptinotarsa decemlineata, жук колорадский картофельный.
[0063] Способы и композиции, предлагаемые в настоящем документе, могут быть эффективны против Hemiptera, таких как Lygus hesperus, Lygus lineolaris, Lygus pratensis, Lygus rugulipennis Popp, Lygus pabulinus, Calocoris norvegicus, Orthops compestris, Plesiocoris rugicollis, Cyrtopeltis modestus, Cyrtopeltis notatus, Spanagonicus albofasciatus, Diaphnocoris chlorinonis, Labopidicola allii, Pseudatomoscelis seriatus, Adelphocoris rapidus, Poecilocapsus lineatus, Blissus leucopterus, Nysius ericae, Nysius raphanus, Euschistus servus, Nezara viridula, Eurygaster, Coreidae, Pyrrhocoridae, Tinidae, Blostomatidae, Reduviidae и Cimicidae. К представляющим интерес вредителям также относятся Araecerus fasciculatus, ложнослоник кофейный; Acanthoscelides obtectus, зерновка фасолевая; Bruchus rufmanus, зерновка бобовая красноногая; Bruchus pisorum, зерновка гороховая; Zabrotes subfasciatus, зерновка фасолевая мексиканская; Diabrotica balteata, блошка окаймленная; Cerotoma trifurcata, жук-листоед; Diabrotica virgifera, жук кукурузный мексиканский; Epitrix cucumeris, блошка картофельная; Chaetocnema confinis, блошка бататовая; Hypera postica, долгоносик люцерновый; Anthonomus quadrigibbus, цветоед яблонный; Sternechus paludatus, долгоносик стеблевой фасолевый; Hypera brunnipennis, долгоносик египетский люцерновый; Sitophilus granaries, долгоносик амбарный; Craponius inaequalis, долгоносик виноградный; Sitophilus zeamais, долгоносик кукурузный; Conotrachelus nenuphar, долгоносик сливовый; Euscepes postfaciatus, долгоносик бататовый вест-индийский; Maladera castanea, хрущик азиатский садовый; Rhizotrogus majalis, хрущ европейский; Macrodactylus subspinosus, бронзовка золотистая; Tribolium confusum, хрущак мучной малый; Tenebrio obscurus, хрущак темный; Tribolium castaneum, хрущак каштановый; Tenebrio molitor, хрущак мучной большой.
[0064] К нематодам относятся паразитические нематоды, такие как клубеньковые, цистообразующие и ранящие нематоды, включая Heterodera spp., Meloidogyne spp. и Globodera spp.; особенно цистообразующие нематоды, включая, но без ограничения, Heterodera glycines (соевая цистообразующая нематода); Heterodera schachtii (свекловичная цистообразующая нематода); Heterodera avenae (злаковая цистообразующая нематода); и Globodera rostochiensis и Globodera pailida (картофельные цистообразующие нематоды). Ранящие нематоды включают Pratylenchus spp.
[0065] Насекомые-вредители могут быть протестированы в отношении пестицидной активности композиций настоящего изобретения на ранних стадиях развития, например, в виде личинок или других незрелых форм. Насекомые могут быть выращены в полной темноте при температуре от приблизительно 20°C до приблизительно 30°C и относительной влажности от приблизительно 30% до приблизительно 70%. Биотесты могут быть осуществлены, как описано в документе Czapla and Lang (1990) J. Econ. Entomol. 83 (6): 2480-2485. Cмотри также экспериментальную часть в настоящем документе.
III. Кассеты экспрессии
[0066] Полинуклеотиды, кодирующие пестицидные белки, предлагаемые в настоящем документе, могут предлагаться в кассетах экспрессии для экспрессии в представляющем интерес организме. Кассета будет включать в себя 5'- и 3'-регуляторные последовательности, функционально связанные с полинуклеотидом, кодирующим пестицидный полипептид, предлагаемый в настоящем документе, что обеспечивает экспрессию полинуклеотида. Кассета может дополнительно содержать по меньшей мере один дополнительный ген или генетический элемент для совместной трансформации в организм. Если включены дополнительные гены или элементы, компоненты связаны функционально. В качестве альтернативы, дополнительный ген(ы) или элемент(ы) могут предлагаться на нескольких кассетах экспрессии. Такая кассета экспрессии предлагается с множеством сайтов рестрикции и/или сайтов рекомбинации для вставки полинуклеотидов, для того чтобы она находилась под транскрипционной регуляцией регуляторных областей. Кассета экспрессии может дополнительно содержать селектируемый маркерный ген.
[0067] Кассета экспрессии в 5'-3' направлении транскрипции будет включать в себя область инициации транскрипции и трансляции (т.е. промотор), пестицидный полинуклеотид настоящего изобретения и область терминации транскрипции и трансляции (т.е., область терминации), функционирующие в представляющем интерес организме, т.е. растении или бактерии. Промоторы настоящего изобретения способны к направлению или управлению экспрессией кодирующей последовательности в клетке-хозяине. Регуляторные области (т.е. промоторы, области регуляции транскрипции и области терминации трансляции) могут быть эндогенными или гетерологичными для клетки-хозяина или друг для друга. Как используется в настоящем документе, "гетерологичный" по отношению к последовательности представляет собой последовательность, которая происходит от чужеродного вида или, если она из того же вида, существенно модифицирована по сравнению со своей нативной формой в композиции и/или геномном локусе посредством преднамеренного вмешательства человека. Как используется в настоящем документе, химерный ген содержит кодирующую последовательность, функционально связанную с областью инициации транскрипции, которая гетерологична для кодирующей последовательности.
[0068] Удобные области терминации доступны из Ti-плазмиды A. tumefaciens, например, области терминации октопинсинтазы и нопалинсинтазы. Смотри также Guerineau et al. (1991) Mol. Gen. Genet. 262:141-144; Proudfoot (1991) Cell 64:671-674; Sanfacon et al. (1991) GenesDev. 5:141-149; Mogen et al. (1990) Plant Cell 2:1261-1272; Munroe et al. (1990) Gene 91:151-158; Ballas et al. (1989) Nucleic Acids Res. 17:7891-7903; и Joshi et al. (1987) Nucleic Acids Res. 15:9627-9639.
[0069] Дополнительные регуляторные сигналы включают, но без ограничения, сайты инициации транскрипции, операторы, активаторы, энхансеры, другие регуляторные элементы, сайты связывания рибосом, инициаторные кодоны, сигналы терминации и т.п. Смотри, например, патенты США №№ 5039523 и 4853331; EPO 0480762A2; Sambrook et al. (1992) Molecular Cloning: A Laboratory Manual, ed. Maniatis et al. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.), далее в данном документе "Sambrook 11"; Davis et al., eds. (1980) Advanced Bacterial Genetics (Cold Spring Harbor Laboratory Press), Cold Spring Harbor, N.Y., и приведенные там ссылки.
[0070] При получении кассеты экспрессии можно манипулировать различными фрагментами ДНК, таким образом, чтобы обеспечивать для последовательностей ДНК правильную ориентацию и, в соответствующих случаях, правильную рамку считывания. С этой целью можно использовать адаптеры или линкеры для соединения фрагментов ДНК, или можно задействовать другие манипуляции для обеспечения удобных сайтов рестрикции, удаления избыточной ДНК, удаления сайтов рестрикции и т.п. С этой целью можно использовать мутагенез in vitro, восстановление праймеров, рестрикцию, отжиг, повторные замены, например, транзиции и трансверсии.
[0071] При осуществлении настоящего изобретения на практике можно использовать ряд промоторов. Промоторы могут быть выбраны исходя из желаемого результата. Нуклеиновые кислоты для экспрессии в представляющем интерес организме могут быть объединены с конститутивными, индуцируемыми, тканепредпочтительными или другими промоторами. Смотри, например, промоторы, приведенные в WO 99/43838 и в патентах США №№ 8575425; 7790846; 8147856; 8586832; 7772369; 7534939; 6072050; 5659026; 5608149; 5608144; 5604121; 5569597; 5466785; 5399680; 5268463; 5608142; и 6177611; включенных в настоящий документ посредством ссылки.
[0072] Для экспрессии в растениях конститутивные промоторы также включают промотор CaMV 35S (Odell et al. (1985) Nature 313:810-812); актина риса (McElroy et al. (1990) Plant Cell 2:163-171); убиквитина (Christensen et al. (1989) Plant Mol. Biol. 12:619-632 и Christensen et al. (1992) Plant Mol. Biol. 18:675-689); pEMU (Last et al. (1991) Theor. Appl. Genet. 81:581-588); MAS (Velten et al. (1984) EMBO J. 3:2723-2730). Индуцируемые промоторы включают управляющие экспрессией связанных с патогенезом белков (PR-белков), которые индуцируются после инфицирования патогеном. Смотри, например, документы Redolfi et al. (1983) Neth. J. Plant Pathol. 89:245-254; Uknes et al. (1992) Plant Cell 4:645-656; и Van Loon (1985) Plant Mol. Virol. 4:111-116; и WO 99/43819, включенные в настоящий документ посредством ссылки. Также можно использовать промоторы, которые экспрессируются локально в месте инфицирования патогеном или рядом с ним (Marineau et al. (1987) Plant Mol. Biol. 9:335-342; Matton et al. (1989) Molecular Plant-Microbe Interactions 2:325-331; Somsisch et al. (1986) Proc. Natl. Acad. Sci. USA 83:2427-2430; Somsisch et al. (1988) Mol. Gen. Genet. 2:93-98; и Yang (1996) Proc. Natl. Acad. Sci. USA 93:14972-14977; Chen et al. (1996) Plant J. 10:955-966; Zhang et al. (1994) Proc. Natl. Acad. Sci. USA 91:2507-2511; Warner et al. (1993) Plant J. 3:191-201; Siebertz et al. (1989) Plant Cell 1:961-968; Cordero et al. (1992) Physiol. Mol. Plant Path. 41:189-200; патент США № 5750386 (индуцируемый нематодами); и приведенные там ссылки).
[0073] В конструкциях настоящего изобретения можно использовать индуцируемые раной промоторы. Такие индуцируемые раной промоторы включают в себя промотор pin II (Ryan (1990) Ann. Rev. Phytopath. 28:425-449; Duan et al. (1996) Nature Biotechnology 14:494-498); wun1 и wun2 (патент США № 5428148); win1 и win2 (Stanford et al. (1989) Mol. Gen. Genet. 215:200-208); systemin (McGurl et al. (1992) Science 225:1570-1573); WIP1 (Rohmeier et al. (1993) Plant Mol. Biol. 22:783-792; Eckelkamp et al. (1993) FEBS Letters 323:73-76); гена MPI (Corderok et al. (1994) Plant J. 6(2):141-150); и т.п., которые включены в настоящий документ посредством ссылки.
[0074] Тканепредпочтительные промоторы для применения в настоящем изобретении включают приведенные в документах Yamamoto et al.(1997) Plant J. 12(2):255-265; Kawamata et al. (1997) Plant Cell Physiol. 38(7):792-803; Hansen et al. (1997) Mol. Gen Genet. 254(3):337-343; Russell et al. (1997) Transgenic Res. 6(2):157-168; Rinehart et al. (1996) Plant Physiol. 112(3):1331-1341; Van Camp et al. (1996) Plant Physiol. 112(2):525-535; Canevascini et al. (1996) Plant Physiol. 112(2):513-524; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778; Lam (1994) Results Probl. Cell Differ. 20:181-196; Orozco et al. (1993) Plant Mol Biol. 23(6):1129-1138; Matsuoka et al. (1993) Proc Natl. Acad. Sci. USA 90(20):9586-9590; и Guevara-Garcia et al. (1993) Plant J. 4(3):495-505.
[0075] Лист-предпочтительные промоторы включают приведенные в документах Yamamoto et al. (1997) Plant J. 12(2):255-265; Kwon et al. (1994) Plant Physiol. 105:357-67; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778; Gotor et al. (1993) Plant J. 3:509-18; Orozco et al. (1993) Plant Mol. Biol. 23(6):1129-1138; и Matsuoka et al. (1993) Proc. Natl. Acad. Sci. USA 90(20):9586-9590.
[0076] Корнепредпочтительные промоторы известны и включают описанные в документах Hire et al. (1992) Plant Mol. Biol. 20(2):207-218 (ген соевой корнеспецифической глутаминсинтетазы); Keller and Baumgartner (1991) Plant Cell 3(10):1051-1061 (корнеспецифический управляющий элемент); Sanger et al. (1990) Plant Mol. Biol. 14(3):433-443 (ген маннопинсинтазы (MAS) Agrobacterium tumefaciens); и Miao et al. (1991) Plant Cell 3(1):11-22 (цитозольная глутаминсинтетаза (GS)); Bogusz et al. (1990) Plant Cell 2(7):633-641; Leach and Aoyagi (1991) Plant Science (Limerick) 79(1):69-76 (rolC и rolD); Teeri et al. (1989) EMBO J. 8(2):343-350; Kuster et al. (1995) Plant Mol. Biol. 29(4):759-772 (промотор гена VfENOD-GRP3); и Capana et al. (1994) Plant Mol. Biol. 25(4):681-691 (промотор rolB). Смотри также патенты США №№ 5837876; 5750386; 5633363; 5459252; 5401836; 5110732; и 5023179.
[0077] "Семяпредпочтительные" промоторы включают как "семяспецифические" промоторы (эти промоторы активны во время развития семян, например, промоторы запасных белков семян), так и промоторы "прорастания семян" (эти промоторы активны во время прорастания семян). Смотри Thompson et al. (1989) BioEssays 10:108. Семяпредпочтительные промоторы включают, но без ограничения, Cim1 (цитокинин-индуцированное сообщение); cZ19B1 (кукурузный зеин 19 кДа); milps (миоинозитол-1-фосфатсинтаза) (смотри WO 00/11177 и патент США № 6225529). Гамма-зеин является эндоспермспецифическим промотором. Глобулин-1 (Glb-1) является типичным эмбриоспецифическим промотором. Для двудольных семяспецифические промоторы включают, но без ограничения, промоторы β-фазеолина фасоли, напина, β-конглицинина, лектина сои, круциферина и т.п. Для однодольных семяспецифические промоторы включают, но без ограничения, промоторы кукурузного зеина 15 кДа, зеина 22 кДа, зеина 27 кДа, гамма-зеина, waxy, shrunken-1, shrunken-2, глобулина-1 и т.д. Смотри также WO 00/12733, где раскрыты семяпредпочтительные промоторы из генов end1 и end2.
[0078] Для экспрессии в бактериальном хозяине в данной области техники хорошо известны промоторы, которые функционируют в бактериях. Такие промоторы включают любой из известных промоторов генов кристаллических белков, включая промоторы любого из пестицидных белков настоящего изобретения и промоторы, специфические для сигма-факторов B. thuringiensis. В качестве альтернативы, промоторы генов, кодирующих полученные мутагенозом или рекомбинантные кристаллические белки, могут быть рекомбинантно сконструированы и использованы для стимуляции экспрессии новых генных сегментов, раскрытых в настоящем документе.
[0079] Кассета экспрессии может также содержать селектируемый маркерный ген для селекции трансформированных клеток. Селектируемые маркерные гены используют для селекции трансформированных клеток или тканей. Маркерные гены включают гены, кодирующие резистентность к антибиотикам, такие как кодирующие неомицинфосфотрансферазу II (NEO) и гигромицинфосфотрансферазу (HPT), а также гены, придающие резистентность к гербицидным соединениям, таким как глюфосинат аммония, бромоксинил, имидазолиноны и 2,4-дихлорфеноксиацетат (2,4-D). Известны дополнительные селектируемые маркеры, и любой из них можно использовать при реализации настоящего изобретения. Смотри, например, документ PCT/US2015/066648, поданный 18 декабря 2015 г., включенный в настоящий документ посредством ссылки во всей полноте, который раскрывает последовательности резистентности к глюфосинату, которые можно использовать в качестве селектируемых маркеров.
IV. Способы, клетки-хозяева и клетки растений
[0080] Как указано, конструкции ДНК, содержащие нуклеотидные последовательности, кодирующие пестицидные белки или их активные варианты или фрагменты, можно использовать для трансформации представляющих интерес растений или других представляющих интерес организмов. Способы трансформации включают в себя введение в растение нуклеотидной конструкции. под "введением" понимается введение нуклеотидной конструкции в растение или другую клетку-хозяина таким образом, что конструкция получает доступ к внутренней части клетки растения или клетки-хозяина. Способы настоящего изобретения не требуют конкретного способа введения нуклеотидной конструкции в растение или клетку-хозяина, а только чтобы нуклеотидная конструкция получила доступ к внутренней части по меньшей мере одной клетки растения или организма-хозяина. Способы введения нуклеотидных конструкций в растения и другие клетки-хозяева известны в данной области техники и включают, но без ограничения, способы стабильной трансформации, способы временной трансформации и опосредованные вирусами способы.
[0081] Данные способы приводит к трансформированным организмам, таким как растение, включая цельные растения, а также органы растений (например, листья, стебли, корни и т.д.), семена, клетки растений, побеги, зародыши и их потомство. Клетки растения могут быть дифференцированными или не дифференцированными (например, каллус, клетки суспензионных культур, протопласты, клетки листьев, клетки корней, клетки луба, пыльца).
[0082] "Трансгенные растения", или "трансформированные растения", или "стабильно трансформированные" растения, или клетки, или ткани обозначает растения, в которые включен или интегрирован полинуклеотид, кодирующий по меньшей мере один пестицидный полипептид настоящего изобретения. Понятно, что другие экзогенные или эндогенные последовательности нуклеиновой кислоты или фрагменты ДНК могут также быть включены в клетку растения. Трансформация, опосредованная Agrobacterium или биолистикой, остается двумя преимущественно используемыми подходами. При этом, трансформация может быть осуществлена посредством инфицирования, трансфицирования, микроинъекции, электропорации, микропроецирования, биолистики или бомбардировки частицами, электропорации, кварцевых/углеродных волокон, опосредования ультразвуком, опосредования ПЭГ, соосаждения фосфатов кальция, метода поликатиона ДМСО, процедуры с ДЭАЭ-декстраном, опосредования Agrobacterium и вирусами (каулимовирусами, геминивирусами, РНК-вирусами растений), опосредования липосомами и т.п.
[0083] Протоколы трансформации, а также протоколы введения полипептидов или полинуклеотидных последовательностей в растения могут варьироваться в зависимости от типа растения или клетки растения, предназначенных для трансформации, т.е. являются ли они однодольными или двудольными. Способы трансформации известны в данной области техники и включают приведенные в патентах США №№ 8575425; 7692068; 8802934; 7541517; которые включены в настоящий документ посредством ссылки. Смотри также, Rakoczy-Trojanowska, M. (2002) Cell Mol Biol Lett. 7:849-858; Jones et al. (2005) Plant Methods 1:5; Rivera et al. (2012) Physics of Life Reviews 9:308-345; Bartlett et al. (2008) Plant Methods 4:1-12; Bates, G.W. (1999) Methods in Molecular Biology 111:359-366; Binns and Thomashow (1988) Annual Reviews in Microbiology 42:575-606; Christou, P. (1992) The Plant Journal 2:275-281; Christou, P. (1995) Euphytica 85:13-27; Tzfira et al. (2004) TRENDS in Genetics 20:375-383; Yao et al. (2006) Journal of Experimental Botany 57:3737-3746; Zupan and Zambryski (1995) Plant Physiology 107:1041-1047; Jones et al. (2005) Plant Methods 1:5.
[0084] Трансформация может приводить к стабильному или временному введению нуклеиновой кислоты в клетку. Под "стабильной трансформацией" понимается, что нуклеотидная конструкция, вводимая в клетку-хозяина, интегрируется в геном клетки-хозяина и может быть унаследована ее потомством. Под "временной трансформацией" понимается, что полинуклеотид вводится в клетку-хозяина и не интегрируется в геном клетки-хозяина.
[0085] Способы трансформации хлоропластов известны в данной области техники. Смотри, например, Svab et al. (1990) Proc. Nail. Acad. Sci. USA 87:8526-8530; Svab and Maliga (1993) Proc. Natl. Acad. Sci. USA 90:913-917; Svab and Maliga (1993) EMBO J. 12:601-606. Способ основан на доставке с помощью генной пушки ДНК, содержащей селектируемый маркер, и направлении ДНК в геном пластид посредством гомологичной рекомбинации. Кроме того, трансформация пластид может быть достигнута с помощью трансактивации молчащего трансгена, несомого пластидами, посредством тканепредпочтительной экспрессии закодированной в ядре и направленной на пластиды РНК-полимеразы. О такой системе сообщалось в документе McBride et al. (1994) Proc. Natl. Acad. Sci. USA 91:7301-7305.
[0086] Клетки, которые были трансформированы, могут вырасти в растения в соответствии с обычными способами. Смотри, например, McCormick et al. (1986) Plant Cell Reports 5:81-84. Эти растения могут затем быть выращены и опылены или тем же самым трансформированным штаммом, или другими штаммами, и может быть идентифицирован итоговый гибрид, имеющий конститутивную экспрессию желаемой фенотипической характеристики. Могут быть выращены два или более поколения, чтобы гарантировать, что экспрессия желаемой фенотипической характеристики стабильно сохраняется и наследуется, и затем собирают семена, чтобы гарантировать достижение экспрессии желаемой фенотипической характеристики. Таким образом, настоящее изобретение предлагает трансформированное семя (также называемое "трансгенным семенем"), в котором нуклеотидная конструкция настоящего изобретения, например, кассета экспрессии настоящего изобретения, стабильно включена в его геном.
[0087] В конкретных вариантах осуществления последовательности, предлагаемые в настоящем документе, могут быть направлены на конкретные сайты в геноме клетки-хозяина или клетки растения. Такие способы включают, но без ограничения, мегануклеазы, сконструированные против представляющей интерес геномной последовательности растения (D'Halluin et al. 2013 Plant Biotechnol J); CRISPR-Cas9, TALEN и другие методы точного редактирования геномов (Feng, et al. Cell Research 23:1229-1232, 2013, Podevin, et al.Trends Biotechnology, опубликовано онлайн, 2013, Wei et al., J Gen Genomics, 2013, Zhang et al (2013) WO 2013/026740); сайт-специфическую рекомбинацию Cre-lox (Dale et al. (1995) Plant J 7:649-659; Lyznik, et al. (2007) Transgenic Plant J 1:1-9); рекомбинацию FLP-FRT (Li et al. (2009) Plant Physiol 151:1087-1095); опосредованную Bxb1 интеграцию (Yau et al.Plant J (2011) 701:147-166); опосредованную цинковыми пальцами интеграцию (Wright et al. (2005) Plant J 44:693-705; Cai et al. (2009) Plant Mol Biol 69:699-709); и гомологичную рекомбинацию (Lieberman-Lazarovich and Levy (2011) Methods Mol Biol 701: 51-65; Puchta (2002) Plant Mol Biol 48:173-182).
[0088] Последовательность, предлагаемую в настоящем документе, можно использовать для трансформации любого вида растений, включая, но без ограничения, однодольные и двудольные. К примерам представляющих интерес растений относятся, но без ограничения, кукуруза (маис), сорго, пшеница, подсолнечник, помидор, крестоцветные, перцы, картофель, хлопок, рис, соя, сахарная свекла, сахарный тростник, табак, ячмень и масличный рапс, Brassica sp., люцерна, рожь, просо, сафлор, арахис, батат, маниока, кофе, кокос, ананас, цитрусовые, какао, чай, банан, авокадо, инжир, гуава, манго, олива, папайя, кешью, макадамия, миндаль, овес, овощи, декоративные растения и хвойные.
[0089] К овощам относятся, но без ограничения, помидоры, салат, зеленые бобы, лимские бобы, горох и члены рода Curcumis, такие как огурец, канталупа и мускусная дыня. К декоративным растениям относятся, но без ограничения, азалия, гортензия, гибискус, розы, тюльпаны, нарциссы, петунии, гвоздика, пуансеттия и хризантема. Предпочтительно, растения настоящего изобретения представляют собой культурные растения (например, маис, сорго, пшеница, подсолнечник, помидор, крестоцветные, перцы, картофель, хлопок, рис, соя, сахарная свекла, сахарный тростник, табак, ячмень, масличный рапс и т.д.).
[0090] Как используется в настоящем документе, термин растение включает клетки растений, протопласты растений, тканевые культуры клеток растений, из которых растения можно регенерировать, каллус растений, поросль растений и клетки растений, которые являются интактными в растениях или частях растений, таких как зародыши, пыльца, семяпочки, семена, листья, цветы, ветки, плоды, зерна, колосья, початки, шелуха, стебли, корни, кончики корней, пыльники и т.п. Под зерном понимается зрелое семя, произведенное коммерческими сельхозпроизводителями с другой целью, чем выращивание или воспроизводство вида. Потомство, варианты и мутанты регенерированных растений также включены в объем настоящего изобретения, при условии, что эти части содержат введенные полинуклеотиды. Кроме того, предлагается обработанный растительный продукт или побочный продукт, который сохраняет последовательности, раскрытые в настоящем документе, включая например, соевый шрот.
[0091] В другом варианте осуществления гены, кодирующие пестицидные белки, можно использовать для трансформации патогенных организмов насекомых. Такие организмы включают бакуловирусы, грибы, простейшие, бактерии и нематоды. Могут быть выбраны микроорганизмы-хозяева, о которых известно, что они занимают "фитосферу" (филлоплан, филлосферу, ризосферу и/или ризоплан) одной или нескольких представляющих интерес культур. Эти микроорганизмы выбирают таким образом, чтобы они были способны успешно конкурировать в конкретной среде с микроорганизмами дикого типа, обеспечивать стабильное сохранение и экспрессию гена, экспрессирующего пестицидный белок и, желательно, обеспечивать улучшенную защиту пестицида от разрушения окружающей средой и инактивации.
[0092] Такие микроорганизмы включают археи, бактерии, водоросли и грибы. Особый интерес представляют такие микроорганизмы, как бактерии, например Bacillus, Pseudomonas, Erwinia, Serratia, Klebsiella, Xanthomonas, Streptomyces, Rhizobium, Rhodopseudomonas, Methylius, Agrobacterium, Acetobacter, Lactobacillus, Arthrobacter, Azotobacter, Leuconostoc и Alcaligenes. Грибы включают дрожжи, например Saccharomyces, Cryptococcus, Kluyveromyces, Sporobolomyces, Rhodotorula и Aureobasidium. Особый интерес представляют такие фитосферные бактериальные виды, как Pseudomonas syringae, Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens, Acetobacter xylinum, Agrobacteria, Rhodopseudomonas spheroides, Xanthomonas campestris, Rhizobium melioti, Alcaligenes entrophus, Clavibacter xyli и Azotobacter vinlandir, и такие фитосферные виды дрожжей, как Rhodotorula rubra, R. glutinis, R. marina, R. aurantiaca, Cryptococcus albidus, C. diffluens, C. laurentii, Saccharomyces rosei, S. pretoriensis, S. cerevisiae, Sporobolomyces rosues, S. odorus, Kluyveromyces veronae, Aureobasidium pollulans, Bacillus thuringiensis, Escherichia coli, Bacillus subtilis и т.п.
[0093] Примеры прокариот, как грамотрицательных, так и грамположительных, включают Enterobacteriaceae, такие как Escherichia, Erwinia, Shigella, Salmonella и Proteus; Bacillaceae; Rhizobiceae, такие как Rhizobium; Spirillaceae, такие как фотобактерии, Zymomonas, Serratia, Aeromonas, Vibrio, Desulfovibrio, Spirillum; Lactobacillaceae; Pseudomonadaceae, такие как Pseudomonas и Acetobacter; Azotobacteraceae и Nitrobacteraceae. Грибы включают фикомицеты и аскомицеты, например дрожжи, такие как Saccharomyces и Schizosaccharomyces; и базидиомицетные дрожжи, такие как Rhodotorula, Aureobasidium, Sporobolomyces и т.п.
[0094] Гены, кодирующие пестицидные белки, могут быть введены посредством электротрансформации, ПЭГ-индуцированной трансформации, теплового шока, трансдукции, конъюгации и т.п. В частности, гены, кодирующие пестицидные белки, могут быть клонированы в челночный вектор, например pHT3101 (Lerecius et al. (1989) FEMS Microbiol. Letts. 60: 211-218). Челночный вектор pHT3101, содержащий кодирующую последовательность для конкретного гена пестицидного белка, может, например, быть трансформирован в колонизирующие корни Bacillus посредством электропорации (Lerecius et al. (1989) FEMS Microbiol. Letts. 60: 211-218).
[0095] Системы экспрессии могут быть сконструированы таким образом, чтобы пестицидные белки секретировались из цитоплазмы грамотрицательных бактерий, посредством слияния подходящего сигнального пептида с аминоконцевой областью пестицидного белка. Сигнальные пептиды, распознаваемые E. Coli, включают белок OmpA (Ghrayeb et al. (1984) EMBO J, 3: 2437-2442).
[0096] Пестицидные белки и их активные варианты могут подвергаться ферментации в бактерии-хозяине, и полученные бактерии обрабатывают и применяют в качестве микробного опрыскивания так же, как применяют штаммы Bacillus thuringiensis в качестве инсектицидных опрыскиваний. В случае пестицидного белка(ов), который секретируется из Bacillus, сигнал секреции подвергают удалению или мутации с использованием процедур, известных в данной области техники. Такие мутации и/или делеции предотвращают секрецию пестицидного белка(ов) в среду для роста в процессе ферментации. Пестицидные белки сохраняются в клетке, а затем клетки обрабатывают для получения инкапсулированных пестицидных белков.
[0097] В качестве альтернативы, пестицидные белки получают посредством введения гетерологичных генов в клетку-хозяина. Экспрессия гетерологичного гена приводит, непосредственно или опосредовано, к внутриклеточному производству и сохранению пестицида. Затем эти клетки обрабатывают в условиях, которые продлевают активность токсина, полученного в клетке, когда клетку применяют в среде целевого вредителя(ей). Полученный продукт сохраняет токсичность токсина. Эти естественным образом инкапсулированные пестицидные белки могут затем быть составлены в соответствии с обычными способами для применения в среде, служащей пристанищем для целевого вредителя, например почве, воде и листве растений. Смотри, например, патент США № 6468523 и публикацию США № 20050138685 и приведенные там ссылки. В настоящем изобретении трансформированный микроорганизм (который включает целые организмы, клетки, спору(ы), пестицидный белок (белки), пестицидный компонент(ы), воздействующий на вредителей компонент(ы), мутант(ы), живые или мертвые клетки и клеточные компоненты, включая смеси живых и мертвых клеток и клеточных компонентов и включая разрушенные клетки и клеточные компоненты) или выделенный пестицидный белок могут быть составлены с приемлемым носителем в пестицидную или сельскохозяйственную композицию(и), то есть, например, суспензию, раствор, эмульсию, порошок для опыления, диспергируемую гранулу, смачиваемый порошок и эмульгируемый концентрат, аэрозоль, пропитанную гранулу, адъювант, пригодную для нанесения пасту, а также включения, например, в полимерные вещества.
[0098] Сельскохозяйственные композиции могут содержать полипептид, рекомбиногенный полипептид или их вариант или фрагмент, как раскрыто в настоящем документе. Сельскохозяйственная композиция, раскрытая в настоящем документе, может быть применена в окружающей растение среде или области его выращивания, или нанесена на растение, часть растения, клетку растения или семя.
[0099] Такие композиции, раскрытые выше, могут быть получены путем добавления поверхностно-активного вещества, инертного носителя, консерванта, увлажнителя, стимулятора питания, аттрактанта, инкапсулирующего средства, связующего вещества, эмульгатора, красителя, вещества для защиты от УФ, буфера, средства повышения текучести или удобрений, доноров микроэлементов или других препаратов, которые влияют на рост растения. Один или несколько агрохимикатов, включая, но без ограничения, гербициды, инсектициды, фунгициды, бактерициды, нематоциды, моллюскициды, акарициды, регуляторы роста растений, средства для увеличения урожая и удобрения, могут быть объединены с носителями, поверхностно-активными веществами или адъювантами, обычно используемыми в области техники составления, или другими компонентами для облегчения манипулирования и применения продукта для конкретных целевых вредителей. Подходящие носители и адъюванты могут представлять собой твердое вещество или жидкость и соответствуют веществам, обычно применяемым в способах составления, например, природным или регенерированным минеральным веществам, растворителям, диспергирующим средствам, смачивающим средствам, придающим клейкость средствам, связующим веществам или удобрениям. Активные ингредиенты настоящего изобретения обычно применяются в форме композиций и могут быть нанесены на подлежащие обработке площадь под культурой, растение или семя. Например, композиции настоящего изобретения могут быть нанесены на зерно при подготовке к хранению или во время хранения в зернохранилище или силосохранилище и т.д. Композиции настоящего изобретения могут быть применены одновременно или последовательно с другими соединениями. Способы применения активного ингредиента настоящего изобретения или агрохимической композиции настоящего изобретения, которая содержит по меньшей мере один из пестицидных белков, производимых бактериальными штаммами настоящего изобретения, включают, но без ограничения, внекорневую подкормку, дражирование семян и нанесение на почву. Количество нанесений и скорость нанесения зависят от интенсивности инфестации соответствующим вредителем.
[0100] Подходящие поверхностно-активные средства включают, но без ограничения, анионные соединения, такие как карбоксилат, например, металла; карбоксилат длинноцепочечной жирной кислоты; N-ацилсаркозинат; моно- или диэфиры фосфорной кислоты с этоксилатами жирных спиртов или соли таких эфиров; сульфаты жирных спиртов, такие такой как додецилсульфат натрия, октадецилсульфат натрия или цетилсульфат натрия; этоксилированные сульфаты жирных спиртов; этоксилированные сульфаты алкилфенолов; лигнинсульфонаты; нефтяные сульфонаты; алкиларилсульфонаты, такие как алкилбензолсульфонаты или низшие алкилнафталинсульфонаты, например бутилнафталинсульфонат; соли сульфонированных нафталинформальдегидных конденсатов; соли сульфонированных фенолформальдегидных конденсатов; более сложные сульфонаты, такие амидсульфонаты, например сульфонированный продукт конденсации олеиновой кислоты и N-метилтаурина; или диалкилсульфосукцинаты, например сульфонат натрия диоктилсукцината. Неионогенные средства включают продукты конденсации эфиров жирных кислот, жирных спиртов, амидов жирных кислот или жирных алкил- или алкенилзамещенных фенолов с этиленоксидом, жирные эфиры простых эфиров многоатомных спиртов, например сорбитановые эфиры жирных кислот, продукты конденсации таких эфиров с этиленоксидом, например полиоксиэтиленсорбитановые эфиры жирных кислот, блок-сополимеры этиленоксида и пропиленоксида, ацетиленгликоли, такие как 2,4,7,9-тетраэтил-5-децин-4,7-диол, или этоксилированные ацетиленгликоли. Примеры катионного поверхностно-активного средства включают, например, алифатический моно-, ди- или полиамин, такой как ацетат, нафтенат или олеат; или кислородсодержащий амин, такой как аминоксид полиоксиэтиленалкиламина; связанный с амидом амин, полученный конденсацией карбоновой кислоты с ди- или полиамином; или соль четвертичного аммония.
[0101] Примеры инертных материалов включают, но без ограничения, неорганические минералы, такие как каолин, филлосиликаты, карбонаты, сульфаты, фосфаты, или ботанические материалы, такие как пробка, истолченные кукурузные початки, арахисовая шелуха, рисовая шелуха и скорлупа грецкого ореха.
[0102] Композиции настоящего изобретения могут иметь форму, подходящую для непосредственного применения, или иметь вид концентрата первичной композиции, который нужно разбавлять подходящим количеством воды или другого разбавителя перед применением. Концентрация пестицидов будет изменяться в зависимости от природы конкретной композиции, в частности от того, является ли она концентратом или будет применяться непосредственно. Композиция содержит от 1 до 98% твердого или жидкого инертного носителя и от 0 до 50% или от 0,1 до 50% поверхностно-активного вещества. Эти композиции могут быть введены в указанном для коммерческого продукта количестве, например приблизительно 0,01-5,0 фунтов на акр в сухой форме и приблизительно 0,01-10 пинт на акр в жидкой форме.
[0103] В другом варианте осуществления композиции, а также трансформированные микроорганизмы и пестицидные белки, предлагаемые в настоящем документе, могут быть обработаны перед составлением для продления пестицидной активности при применении в среде целевого вредителя, при условии, что предварительная обработка не повредит пестицидной активности. Такая обработка может осуществляться химическими и/или физическими средствами при условии, что обработка не окажет вредного воздействия на свойства композиции(ий). Примеры химических реагентов включают, но без ограничения, галогенирующие средства; альдегиды, такие как формальдегид и глутаральдегид; противоинфекционные средства, такие как зефиранхлорид; спирты, такие как изопропанол и этанол; и гистологические фиксаторы, такие как фиксатор Буэна и фиксатор Хелли (смотри, например, Humason (1967) Animal Tissue Techniques (W.H. Freeman and Co.)).
[0104] В одном аспекте вредители могут быть уничтожены, или может быть уменьшено их количество на заданной площади посредством нанесения пестицидных белков настоящего изобретения на эту площадь. В качестве альтернативы, пестицидные белки могут быть профилактически нанесены на окружающую территорию для предотвращения инфестации восприимчивым вредителем. Предпочтительно, вредитель поглощает или входит в контакт с пестицидно эффективным количеством полипептида. Под "пестицидно эффективным количеством" понимается количество пестицида, которое может вызывать гибель по меньшей мере одного вредителя или заметно уменьшать рост, питание или нормальное физиологическое развитие вредителя. Это количество будет варьироваться в зависимости от таких факторов, как, например, конкретные подлежащие контролю целевые вредители, конкретные подлежащие обработке окружающая среда, местоположение, растение, культура или сельскохозяйственный участок, условия окружающей среды, и способ, скорость, концентрация, стабильность и количество нанесения пестицидно эффективной полипептидной композиции. Составы или композиции могут также варьироваться в зависимости от климатических условий, экологических соображений и/или частоты применения и/или тяжести инфестации вредителем.
[0105] Активные ингредиенты, как правило, применяют в виде композиций, и они могут быть нанесены на подлежащие обработке площадь под культурой, растение или семя. Поэтому предлагаются способы обеспечения для растения, клетки растения, семени, части растения или обрабатываемой площади эффективного количества сельскохозяйственной композиции, содержащей полипептид, рекомбиногенный полипептид или их активный вариант или фрагмент. Под "эффективным количеством" понимается количество белка или композиции, достаточное для уничтожения или контроля вредителя или приводящее к заметному снижению роста, питания или нормального физиологического развития вредителя. Такое снижение количества вредителя, роста вредителя, питания вредителя или нормального развития вредителя может содержать любое статистически значимое снижение, включая, например, снижение на приблизительно 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 85%, 90%, 95% или больше. Например, композиции могут быть нанесены на зерно при подготовке к хранению или во время хранения в зернохранилище или силосохранилище и т.д. Композиции могут быть применены одновременно или последовательно с другими соединениями. Способы применения активного ингредиента или агрохимической композиции, содержащей по меньшей мере один из полипептидов, рекомбиногенных полипептидов или их вариантов или фрагментов, как раскрыто в настоящем документе, включают, но без ограничения, внекорневую подкормку, дражирование семян и нанесение на почву.
[0106] Предлагаются способы повышения урожайности растения. Данные способы содержат обеспечение растения или клетки растения, экспрессирующих полинуклеотид, кодирующий пестицидную полипептидную последовательность, раскрытую в настоящем документе, и выращивание растения или его семени в поле, инфестированном (или допускающем инфестацию) вредителем, в отношении которого упомянутый полипептид обладает пестицидной активностью. В некоторых вариантах осуществления полипептид обладает пестицидной активностью в отношении чешуекрылого, жесткокрылого, двукрылого, полужесткокрылого вредителя или вредителя-нематоды, и упомянутое поле инфестировано чешуекрылым, полужесткокрылым, жесткокрылым, двукрылым вредителем или вредителем-нематодой. Как определено в настоящем документе, "урожайность" растения относится к качеству и/или количеству биомассы, производимой растением. Под "биомассой" понимается любой измеряемый продукт растения. Увеличением производства биомассы является любое улучшение урожайности измеряемого продукта растения. Повышение урожайности растения имеет несколько коммерческих приложений. Например, увеличение биомассы листьев растения может увеличивать урожайность листовых овощей для потребления человеком или животными. Кроме того, увеличение биомассы листьев можно использовать для увеличения производства фармацевтических или промышленных продуктов растительного происхождения. Увеличение урожайности может включать в себя любое статистически значимое увеличение, включая, но без ограничения, увеличение по меньшей мере на 1%, увеличение по меньшей мере на 3%, увеличение по меньшей мере на 5%, увеличение по меньшей мере на 10%, увеличение по меньшей мере на 20%, увеличение по меньшей мере на 30%, по меньшей мере на 50%, по меньшей мере на 70%, по меньшей мере на 100% или больше урожайности по сравнению с растением, не экспрессирующим пестицидную последовательность. В конкретных способах урожайность растения увеличивается в результате улучшения резистентности растения, экспрессирующего пестицидный белок, раскрытый в настоящем документе, к вредителям. Экспрессия пестицидного белка приводит к снижению способности вредителя инфестировать или питаться.
[0107] Растения также могут быть обработаны одной или несколькими химическими композициями, включая один или несколько гербицидов, инсектицидов или фунгицидов.
[0108] К неограничивающим вариантам осуществления относятся:
[0109] 1. Выделенный полипептид, обладающий инсектицидной активностью, содержащий:
(a) полипептид, содержащий аминокислотную последовательность, выбранную из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398; или
(b) полипептид, содержащий аминокислотную последовательность, имеющую по меньшей мере процентную идентичность последовательности, приведенную в таблице 1, с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398.
[0110] 2. Полипептид в соответствии с вариантом осуществления 1, причем упомянутый полипептид содержит аминокислотную последовательность, приведенную в SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398.
[0111] 3. Полипептид в соответствии с вариантом осуществления 1 или 2, дополнительно содержащий гетерологичные аминокислотные последовательности.
[0112] 4. Композиция, содержащая полипептид в соответствии с вариантами осуществления 1, 2 или 3.
[0113] 5. Рекомбинантная молекула нуклеиновой кислоты, которая кодирует полипептид в соответствии с любым из вариантов осуществления 1-3, причем упомянутая рекомбинантная молекула нуклеиновой кислоты не является природной последовательностью, кодирующей упомянутый полипептид.
[0114] 6. Рекомбинантная нуклеиновая кислота в соответствии с вариантом осуществления 5, причем упомянутая молекула нуклеиновой кислоты представляет собой синтетическую последовательность, сконструированную для экспрессии в растении.
[0115] 7. Рекомбинантная молекула нуклеиновой кислоты в соответствии с вариантом осуществления 5 или 6, причем упомянутая молекула нуклеиновой кислоты функционально связана с промотором, способным к направлению экспрессии в клетке растения.
[0116] 8. Рекомбинантная молекула нуклеиновой кислоты в соответствии с любым из вариантов осуществления 5-7, причем упомянутая молекула нуклеиновой кислоты функционально связана с промотором, способным к направлению экспрессии в бактерии.
[0117] 9. Клетка-хозяин, которая содержит рекомбинантную молекулу нуклеиновой кислоты в соответствии с любым из вариантов осуществления 5-8.
[0118] 10. Клетка-хозяин в соответствии с вариантом осуществления 9, причем упомянутая клетка-хозяин представляет собой бактериальную клетку-хозяина.
[0119] 11. Конструкция ДНК, содержащая промотор, который управляет экспрессией в клетке растения, функционально связанный с рекомбинантной молекулой нуклеиновой кислоты, содержащей:
(a) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность в соответствии с любым из SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398; или
(b) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность, имеющую по меньшей мере процентную идентичность последовательности, приведенную в таблице 1, с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398.
[0120] 12. Конструкция ДНК в соответствии с вариантом осуществления 11, в которой упомянутая нуклеотидная последовательность представляет собой синтетическую последовательность ДНК, сконструированную для экспрессии в растении.
[0121] 13. Вектор, содержащий конструкцию ДНК в соответствии с вариантом осуществления 11 или 12.
[0122] 14. Клетка-хозяин, которая содержит конструкцию ДНК в соответствии с любым из вариантов осуществления 11-13 или вектор в соответствии с вариантом осуществления 13.
[0123] 15. Клетка-хозяин в соответствии с вариантом осуществления 14, причем данная клетка-хозяин является клеткой растения.
[0124] 16. Трансгенное растение, содержащее клетку-хозяина в соответствии с вариантом осуществления 15.
[0125] 17. Композиция, содержащая клетку-хозяина в соответствии с любым из вариантов осуществления 9, 10, 14 или 15.
[0126] 18. Композиция в соответствии с вариантом осуществления 17, причем упомянутую композицию выбирают из группы, состоящей из порошка, пылевидного препарата, пеллета, гранулы, опрыскивания, эмульсии, коллоида и раствора.
[0127] 19. Композиция в соответствии с вариантом осуществления 17 или 18, причем упомянутая композиция содержит от приблизительно 1% до приблизительно 99% по весу упомянутого полипептида.
[0128] 20. Способ контроля популяции вредителей, содержащий приведение упомянутой популяции в контакт с пестицидно эффективным количество композиции в соответствии с любым из вариантов осуществления 4 или 17-19.
[0129] 21. Способ уничтожения популяции вредителей, содержащий приведение упомянутой популяции в контакт с пестицидно эффективным количеством композиции в соответствии с любым из вариантов осуществления 4 или 17-19.
[0130] 22. Способ получения полипептида с пестицидной активностью, содержащий культивирование клетки-хозяина в соответствии с любым из вариантов осуществления 9, 10, 14 или 15 в условиях, в которых экспрессируется молекула нуклеиновой кислоты, кодирующая данный полипептид.
[0131] 23. Растение, имеющее стабильно включенную в его геном конструкцию ДНК, содержащую нуклеотидную последовательность, которая кодирует белок, обладающий пестицидной активностью, причем упомянутая нуклеотидная последовательность содержит:
(a) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность в соответствии с любым из SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398; или
(b) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность, имеющую по меньшей мере процентную идентичность последовательности, приведенную в таблице 1, с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398.
[0132] 24. Трансгенное семя растения в соответствии с вариантом осуществления 23.
[0133] 25. Способ защиты растения от насекомого-вредителя, содержащий экспрессию в растении или его клетке нуклеотидной последовательности, которая кодирует пестицидный полипептид, причем упомянутая нуклеотидная последовательность содержит:
(a) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность в соответствии с любым из SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398; или
(b) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность, имеющую по меньшей мере процентную идентичность последовательности, приведенную в таблице 1, с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398.
[0134] 26. Способ в соответствии с вариантом осуществления 25, в котором упомянутое растение производит пестицидный полипептид, обладающий пестицидной активностью в отношении чешуекрылого или жесткокрылого вредителя.
[0135] 27. Способ повышения урожайности растения, содержащий выращивание в поле растения или его семени, имеющих стабильно включенную в их геном конструкцию ДНК, содержащую промотор, который управляет экспрессией в растении, функционально связанный с нуклеотидной последовательностью, которая кодирует пестицидный полипептид, причем упомянутая нуклеотидная последовательность содержит:
(a) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность в соответствии с любым из SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398; или
(b) нуклеотидную последовательность, которая кодирует полипептид, содержащий аминокислотную последовательность, имеющую по меньшей мере процентную идентичность последовательности, приведенную в таблице 1, с аминокислотной последовательностью, выбранной из группы, состоящей из последовательностей, приведенных в SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397 или 398.
[0136] 28. Предлагается способ получения полинуклеотида, который кодирует улучшенный полипептид, обладающий пестицидной активностью, причем данный улучшенный полипептид имеет по меньшей мере одно улучшенное свойство для любой из SEQ ID NO: 1-398, содержащий:
(a) рекомбинацию множества родительских полинуклеотидов, содержащих SEQ ID NO: 1-398 или их активный вариант или фрагмент, для получения библиотеки рекомбинантных полинуклеотидов, кодирующих рекомбинантные пестицидные полипептиды;
(b) скрининг библиотеки для идентификации рекомбинантного полинуклеотида, который кодирует улучшенный рекомбинантный пестицидный полипептид, который обладает усиленным свойством, улучшенным по сравнению с родительским полинуклеотидом;
(c) восстановление рекомбинантного полинуклеотида, который кодирует улучшенный рекомбинантный пестицидный полипептид, идентифицированный в (b); и
(d) повторение этапов (a), (b) и (c) с использованием рекомбинантного полинуклеотида, восстановленного на этапе (c), в качестве одного из множества родительских полинуклеотидов при повторении этапа (a).
[0137] Следующие примеры предлагаются в качестве иллюстрации, а не в качестве ограничения.
ЭКСПЕРИМЕНТАЛЬНАЯ ЧАСТЬ
Эксперимент 1: Открытие новых генов с помощью секвенирования и анализа ДНК
[0138] Микробные культуры выращивали в жидкой культуре в стандартных лабораторных средах. Культуры выращивали до насыщения (от 16 до 24 часов) до получения ДНК. ДНК экстрагировали из бактериальных клеток посредством лизиса детергентом, а затем связывания с кварцевой матрицей и промывания буфером с этанолом. Очищенную ДНК извлекали из кварцевой матрицы с помощью умеренно щелочного водного буфера.
[0139] ДНК для секвенирования тестировали на чистоту и концентрацию с помощью спектрофотометрии. Библиотеки секвенирования были получены с использованием набора для получения библиотек Nextera XT в соответствии с протоколом производителя. Данные последовательностей получали на HiSeq 2000 в соответствии с протоколом из Illumina HiSeq 2000 System User Guide.
[0140] Из данных секвенирования собирали черновые геномы с использованием программного пакета CLC Bio Assembly Cell. После сборки осуществляли распознавание генов с помощью нескольких способов и получаемые последовательности генов опрашивали для идентификации новых гомологов пестицидных генов. Новый гены идентифицировали с помощью BLAST, по композиции доменов и с помощью попарного выравнивания по целевому набору пестицидных генов. Сводная информация о таких последовательностях приведена в таблице 1.
[0141] Идентифицированные при поиске гомологии гены амплифицировали из бактериальной ДНК с помощью ПЦР и клонировали в бактериальные векторы экспрессии, содержащие слитые внутрирамочные метки очистки. Клонированные гены экспрессировали в E. coli и очищали с помощью колоночной хроматографии. Очищенные белки оценивали в исследованиях биопроб питания насекомых для идентификации активных белков.
[0142] Биопробы питания насекомых были выполнены с использованием искусственного питания из зародышей пшеницы и агара, на которые наносили очищенный белок посредством поверхностной обработки. Личинки насекомых получали обработанное питание, и их смертность отслеживали.
[0143] Биопробы питания насекомых были выполнены с использованием жидкого питания с сахарозой, содержащегося в мембранном пакетике, к которому добавляли очищенный белок. Нимф насекомых оставляли питаться на пакетике с питанием, и их смертность отслеживали. Протестированными в биопробах насекомыми были щитник коричневый (BSB), Euschistus servus,и щитник зеленый южный (SGSB), Nezara viridula.
Пример 2. Гетерологичная экспрессия в E. coli
[0144] Каждую открытую рамку считывания, приведенную в таблицах 3 и 4, клонировали в вектор экспрессии E. Coli, содержащий мальтозосвязывающий белок (pMBP). Вектор экспрессии трансформировали в BL21*RIPL. Культуру LB, дополненную карбенициллином, инокулировали одной колонией и выращивали в течение ночи при 37°C, с помощью 0,5% выращиваемой в течение ночи культуры инокулировали свежую культуру и выращивали до логарифмической фазы при 37°C. Культуру индуцировали с помощью 250 мМ IPTG в течение 18 часов при 16°C. Клетки осаждали и ресуспендировали в 10 мМ Tris pH7,4 и 150 мМ NaCl, дополненном ингибиторами протеаз. Белковую экспрессия оценивали с помощью ДСН-ПААГ.
Пример 3. Пестицидная активность в отношении жесткокрылых и чешуекрылых
[0145] Экспрессия белка: каждую последовательность, приведенную в таблице 3, экспрессировали в E. Coli, как описано в примере 2. Инокулировали 400 мл LB и выращивали до OD 600, равной 0,6. Культуру индуцировали с помощью 0,25 мМ IPTG в течение ночи при 16°C. Клетки осаждали центрифугированием, и клеточный осадок ресуспендировали в 5 мл буфера. Ресуспендирование обрабатывали ультразвуком в течение 2 мин на льду.
[0146] Биопроба: яйца совки травяной (FAW), совки кукурузной (CEW), мотылька кукурузного (ECB), огневки кукурузной юго-западной (SWCB) и моли капустной (DBM или Px) были приобретены в коммерческом инсектарии (Benzon Research Inc., Carlisle, PA). Яйца FAW, CEW, ECB и BCW инкубировали до момента, когда вылупление должно было произойти в течение 12 часов анализа. SWCB и DBM вводили в анализ в виде новорожденных личинок. Анализы проводили в 24-луночных планшетах, содержащих питание, предназаначенное для многих видов чешуекрылых (Southland Products Inc., Lake Village, AR). Образцы обработанного ультразвуком лизата наносили на поверхность питания (поверх питания) и оставляли для испарения и впитывания в питание. Для CEW, FAW, BCW, ECB и SWCB 125 мкл обработанного ультразвуком лизата добавляли на поверхность питания и сушили. Для DBM на поверхность питания добавляли 50 мкл разведенного 1:2 обработанного ультразвуком лизата. Планшеты для биопроб запечатывали герметизирующей пленкой для планшетов с проделанными точечными отверстиями. Планшеты инкубировали при 26°C при относительной влажности (RH) 65% с циклом день:ночь 16:8 в Percival в течение 5 дней. Пробы оценивали по уровню смертности, ингибированию роста и ингибированию питания.
[0147] Для биопробы западного кукурузного жука белковую конструкцию/лизат оценивали в биопробе насекомого посредством дозирования объема 60 мкл поверх поверхности питания в лунки 24-луночного планшета (Cellstar, 24-луночный, Greiner Bio One) и оставляли высохнуть. Каждая лунка содержала 500 мкл питания (Marrone et al., 1985). От пятнадцати до двадцати новорожденных личинок вносили в каждую лунку с использованием кисти с тонким кончиком, и планшет закрывали мембраной (Viewseal, Greiner Bio One). Биопробу хранили при температуре окружающей среды и оценивали в отношении смертности и/или ингибирования роста/питания на день 4.
[0148] Для колорадского картофельного жука (CPB) вырезали из картофельного листа листовой диск пробковым сверлом размера № 8, погружали его в белковую конструкцию/лизат до полного смачивания и помещали поверх фильтровального диска (Millipore, фильтр из стекловолокна, 13 мм). 60 мкл dH2O добавляли к каждому фильтровальному диску и помещали в каждую лунку 24-луночного планшета (Cellstar, 24-луночной, Greiner Bio One). Листовому диску давали высохнуть, и от пяти до семи личинок первой возрастной стадии вносили в каждую лунку с использованием кисти с тонким кончиком. Планшет закрывали мембраной (Viewseal, Greiner Bio One), и в каждой лунке мембраны протыкали маленькое отверстие. Конструкцию оценивали в четырех повторностях, и проводили оценку смертности и повреждения листа на день 3.
[0149] В таблице 3 приведена сводная информация о пестицидной активности различных последовательностей в отношении жесткокрылых и чешуекрылых. Код таблицы: "-" указывает на отсутствие видимой активности; "+" указывает на пестицидную активность; "NT" обозначает отсутствие тестирования; "S" указывает на задержку роста; "SS" указывает на слабую задержку роста; "LF" указывает на сниженное питание, "M" указывает на смертность.
Таблица 3. Сводная информация о пестицидной активности в отношении жесткокрылых и чешуекрылых.
Пример 4. Пестицидная активность в отношении полужесткокрылых
[0150] Экспрессия белка: каждую последовательность, приведенную в таблице 4, экспрессировали в E. Coli, как описано в примере 2. 400 мл LB инокулировали и выращивали до OD 600, раной 0,6. Культуру индуцировали с помощью 0,25 мМ IPTG в течение ночи при 16°C. Клетки осаждали центрифугированием, и клеточный осадок ресуспендировали в 5 мл буфера. Ресуспендирование обрабатывали ультразвуком в течение 2 мин на льду.
[0151] SGSB во второй возрастной стадии получали из коммерческого инсектария (Benzon Research Inc., Carlisle, PA). В биопробе использовали отношение 50% об/об образца обработанного ультразвуком лизата к 20% сахарозе. Растянутый парафильм использовали в качестве подающей мембраны, чтобы подавать SGSB смесь питание/образец. Планшеты инкубировали при 25°C: 21°C, цикл день:ночь 16:8 при 65% RH в течение 5 дней.
[0152] Для каждого образца оценивали смертность. Результаты приведены в таблице 4. Пунктирная линия указывает на отсутствие смертности. Белки, приведенные в таблице 4, продемонстрировали 25% смертность или 50% смертность (как указано) в отношении щитника зеленого южного (1 щитник из 4 умер). Оба отрицательных контроля (пустой вектор, экспрессирующий связывающий домен, и только буфер) продемонстрировали отсутствие смертности (0 щитников из 4).
Таблица 4. Сводная информация о пестицидной активности в отношении полужесткокрылых
Пример 5. Трансформация сои
[0153] Конструкции ДНК, содержащие каждую из SEQ ID NO: 1-398 или их активные варианты или фрагменты, функционально связанные с промотором, активным в растении, клонировали в векторы трансформации и вводили в агробактерии, как описано в заявке PCT/US2015/066702, поданной 18 декабря 2015 г., включенной в настоящий документ посредством ссылки во всей полноте.
[0154] За четыре дня до инокуляции несколько петель агробактерий наносили штрихами на свежий планшет со средой YEP*, дополненной подходящими антибиотиками** (спектиномицином, хлорамфениколом и канамицином). Бактерии выращивают в течение двух дней в темноте при 28°C. Через два дня несколько петель бактерий переносили в 3 мл жидкой среды YEP с антибиотиками в колбе Эрленмейера 125 мл. Колбы оставляли на ротационном шейкере при 250 об/мин при 28°C в течение ночи. За один день до инокуляции 2-3 мл ночной культуры переносили в 125 мл YEP с антибиотиками в колбе Эрленмейера 500 мл. Колбы оставляли на ротационном шейкере при 250 об/мин при 28°C в течение ночи.
[0155] До инокуляции OD бактериальной культуры проверяли как OD 620. OD, составляющая 0,8-1,0, указывает, что культура находится в логарифмической фазе. Культуру центрифугируют при 4000 об/мин в течение 10 минут в пробирках Oakridge. Супернатант удаляют, а осадок ресуспендируют в объеме среды Soybean Infection (SI) для достижения желаемой OD. Культуры хранят с периодическим перемешиванием, пока они не потребуются для инокуляции.
[0156] За два или три дня до инокуляции семена сои поверхностно стерилизуют с помощью газообразного хлора. В вытяжном шкафу чашку Петри с семенами помещают в колпак без крышки. К 100 мл отбеливателя в колбе Эрленмейера 250 мл внутри колпака медленно добавляют 1,75 мл 12 н HCl. Крышку немедленно помещают на верхнюю часть колпака. Семена оставляют стерилизоваться в течение 14-16 часов (в течение ночи). Верхнюю часть колпака удаляют, и заменяют крышку чашки Петри. Чашку Петри со стерилизованной поверхностью затем открывают в ламинарном потоке приблизительно на 30 минут для рассеивания оставшегося газообразного хлора.
[0157] Семена пропитывают или стерильной DI водой, или средой Soybean Infection (SI) в течение 1-2 дней. От двадцати до 30 семян покрывают жидкостью в чашке Петри 100×25 мм и инкубируют в темноте при 24°C. После пропитывания непроросшие семена удаляют.
[0158] Семядольные эксплантаты обрабатывают на стерильном бумажном планшете со стерильной фильтровальной бумагой, смоченной с помощью среды SI, используя способы из патента США № 7473822, включенного в настоящий документ посредством ссылки.
[0159] Как правило, на одну обработку высевают 16-20 семядолей. Среду SI, использованную для эксплантатов, удаляют и заменяют на 25 мл культуры агробактерий (OD 620=0,8-20). После погружения всех эксплантатов проводят инокуляцию в течение 30 минут при периодическом вращении чашки. Через 30 минут культуру агробактерий удаляют.
[0160] Планшеты для совместного культивирование получают, накладывая один кусок стерильной бумаги на среду Soybean Co-cultivation (SCC). Без промокания инокулированные семядоли культивируют адаксиальной стороной вниз на фильтровальной бумаге. На каждом планшете может быть культивировано около 20 эксплантатов. Планшеты запечатывают парафильмом и культивируют при 24°C и примерно 120 мкмоль m-2s-1 (в инкубаторе Percival) в течене 4-5 дней.
[0161] После совместного культивирования семядоли промывают 3 раза в 25 мл среды Soybean Wash с 200 мг/л цефотаксима и тиментина. Семядоли промокают стерильной фильтровальной бумагой, а затем переносят в среду Soybean Shoot Induction (SSI). Узловой конец эксплантата слегка вдавливают в среду, оставляя дистальный конец выше поверхности приблизительно под 45°. Не более 10 эксплантатов культивируют на каждом планшете. Планшеты оборачивают лентой Micropore и культивируют в Percival при 24°C и примерно 120 мкмоль m-2s-1.
[0162] Эксплантаты переносят в свежую среду SSI через 14 дней. Возникающие побеги от верхушки побега и семядольных узлов удаляют. Индукция побегов продолжается в течение еще 14 дней в тех же условиях.
[0163] Через 4 недели индукции побегов семядолю отделяют от узлового конца и делают параллельный надрез под участком индукции побега (основание побега). Участок параллельного надреза помещают на среду Soybean Shoot Elongation (SSE), и эксплантаты культивируют в Percival при 24°C и примерно 120 мкмоль m-2s-1. Этот этап повторяют каждые две недели вплоть до 8 недель, пока побеги продолжают удлиняться.
[0164] Когда побеги достигают длины 2-3 см, их переносят в среду Soybean Rooting (SR) в сосуде Plantcon и инкубируют в тех же условиях в течение 2 недель или до тех пор, пока корни не достигнут длины примерно 3-4 см. После этого растения переносят в почву.
[0165] Следует отметить, что все среды, упомянутые для трансформации сои можно найти в документе Paz et al. (2010) Agrobacterium-mediated transformation of soybean and recovery of transgenic soybean plants; Plant Transformation Facility Университета штата Айова, который включен в настоящий документ посредством ссылки во всей полноте. (смотри agron-www.agron.iastate.edu/ptf/protocol/Soybean.pdf).
Пример 6. Трансформация кукурузы
[0166] Початки кукурузы собирали в наилучший момент через 8-12 дней после опыления. Из початков выделяли зародыши, и эти зародыши размером 0,8-1,5 мм являются предпочтительными для использования в трансформации. Зародыши высевали щитком кверху на подходящей инкубационной среде, такой как среда DN62A5S (3,98 г/л солей N6; 1 мл/л (из исходного 1000x раствора) витаминов N6; 800 мг/л L-аспарагина; 100 мг/л миоинозитола; 1,4 г/л L-пролина; 100 мг/л казаминовых кислот; 50 г/л сахарозы; 1 мл/л (из исходного раствора 1 мг/мл) 2,4-D). Однако подходят и известны в данной области техники среды и соли, отличные от DN62A5S. Зародыши инкубируют в течение ночи при 25°C в темноте. Однако по существу нет необходимости в инкубации зародышей в течение ночи.
[0167] Полученные эксплантаты переносят на квадраты сетки (30-40 на планшет), переносят на осмотическую среду приблизительно на 30-45 минут, а затем переносят на пластину для пучковой трансформации (смотри, например публикацию PCT № WO/0138514 и патент США № 5240842). Конструкции ДНК, сконструированные для экспрессии белков GRG настоящего изобретения в клетках растения, вводят с ускорением в ткань растения с использованием аэрозольного пускового ускорителя, используя условия, в целом как описано в публикации PCT № WO/0138514. После пучковой трансформации зародыши инкубируют в течение приблизительно 30 мин на осмотической среде и оставляют на инкубационной среде в течение ночи при 25°C в темноте. Во избежание чрезмерного повреждения трансформированных эксплантатов, их инкубируют в течении по меньшей мере 24 часов до переноса на восстанавливающую среду. Затем зародыши выкладывают на среду восстановительного периода приблизительно на 5 дней, 25°C в темноте, и затем переносят на селекционную среду. Эксплантаты инкубируют в селекционной среде вплоть до восьми недель в зависимости от природы и характеристик конкретной используемой селекции. После селекционного периода полученный каллус переносят на среду созревания зародышей вплоть до обнаружения образования зрелых соматических зародышей. Полученные зрелые соматические зародыши затем помещают под слабым освещением, и инициируют процесс регенерации способами, известными в данной области техники. Полученным побегам дают укорениться на среде для укоренения, и полученные растения переносят в цветочные горшки и размножают как трансгенные растения.
Пример 7. Пестицидная активность в отношении нематод
Анализ in vitro Heterodera glycine (соевая цистообразующая нематода)
[0168] Соевые цистообразующие нематоды распределяли по 96-луночному планшету для анализа при общем объеме 100 мкл и 100 J2 на лунку. Представляющий интерес белок, приведенный в любой из SEQ ID NO: 1-398, распределяли по лункам и выдерживали при комнатной температуре во время оценки. В конце 96-луночный планшет, содержащий SCN J2, подвергали анализу на подвижность. Данные представлены как % ингибирования по сравнению с контролем. Успех определяли как ингибирование, большее или равное 70%.
Анализ Heterodera glycine (соевая цистообразующая нематода) на растении
[0169] Растения сои, экспрессирующие одну или несколько из SEQ ID NO: 1-398, получали, как описано в другом месте в настоящем документе. 3-недельные черенки сои инокулировали с 5000 яиц SCN на растение. Эту инфекцию выдерживали в течение 70 дней и затем собирали для подсчета цист SCN, которые развились на растении. Данные представлены как % ингибирования по сравнению с контролем. Успех определяли как ингибирование, большее или равное 90%.
Анализ in vitro Meloidogyne incognita (галловой нематоды)
[0170] Корневые нематоды распределяли по 96-луночному планшет для анализа при общем объеме 100 мкл и 100 J2 на лунку. Представляющий интерес белок, содержащий любую из SEQ ID NO: 1-398, распределяли по лункам и выдерживали при комнатной температуре во время оценки. В конце 96-луночный планшет, содержащий RKN J2, подвергали анализу на подвижность. Данные представлены как % ингибирования по сравнению с контролем. Успех определяли как ингибирование, большее или равное 70%.
Анализ Meloidogyne incognita (галловой нематоды) на растении
[0171] Растения сои, экспрессирующие одну или несколько из SEQ ID NO: 1-398, получали, как описано в другом месте в настоящем документе. 3-недельные черенки сои инокулировали с 5000 яиц RKN на растение. Эту инфекцию выдерживали в течение 70 дней и затем собирали для подсчета яиц RKN, которые развились на растении. Данные представлены как % ингибирования по сравнению с контролем. Успех определяли как ингибирование, большее или равное 90%.
Пример 8. Дополнительные анализы на пестицидную активность
[0172] Различные полипептиды, приведенные в SEQ ID NO: 1-398, могут быть протестированы в отношении их воздействия в качестве пестицида на вредителя с помощью ряда способов. Одним из таких способов является осуществление анализа питания. В таком анализе питания вредителя подвергают воздействию образца, содержащего или тестируемые соединения, или контрольные образцы. Часто это осуществляют посредством помещения тестируемого материала или подходящего разведения такого материала на материал, который вредитель будет поглощать, такой как искусственное питание. Тестируемый материал может состоять из жидкости, твердого вещества или кашицы. Тестируемый материал может быть помещен на поверхность, и затем оставлен для высыхания. В качестве альтернативы, тестируемый материал может быть смешан с жидким искусственным питанием и затем распределен по камере для анализа. Камера для анализа может представлять собой, например, чашку, тарелку или лунку микротитрационного планшета.
[0173] Анализы для сосущих вредителей (например, тлей) могут включать в себя отделение тестируемого материала от насекомого с помощью перегородки, в идеале элементом, который может быть проткнут сосущими частями ротового аппарата сосущего насекомого, чтобы обеспечить попадание тестируемого материала внутрь. Часто тестируемый материал смешивают со стимулятором питания, таким как сахароза, для стимуляции попадания тестируемого соединения внутрь.
[0174] Другие типы анализов могут включать в себя микроинъекцию тестируемого материала в ротовой аппарат или кишечник вредителя, а также выращивание трансгенных растений с последующим тестированием способности вредителя питаться на трансгенном растении. Тестирование растения может включать в себя выделение обычно потребляемых частей растения, например с помощью небольших клеток, прикрепленных к листу, или выделение целых растений в клетках, содержащих насекомых.
[0175] Другие способы и подходы к анализу вредителей известны в данной области техники, и их можно найти, например, в документе Robertson and Preisler, eds. (1992) Pesticide bioassays with arthropods, CRC, Boca Raton, Fla. В качестве альтернативы, анализы широко представлены в журналах "Arthropod Management Tests" и "Journal of Economic Entomology", или их можно найти с помощью обсуждения с членами Энтомологического общества Америки (ESA). Любую из SEQ ID NO: 1-398 можно экспрессировать и использовать в анализах, приведенных в примерах 3 и 4 в настоящем документе.
[0176] Все публикации и патентные заявки, упомянутые в настоящем описании изобретения, указывают на уровень квалификации специалистов в области техники, к которой относится настоящее изобретение. Все публикации и патентные заявки включены в настоящий документ посредством ссылки в той же степени, как если бы каждая отдельная публикация или патентная заявка была конкретно и отдельно указана как включенная посредством ссылки.
[0177] Хотя вышеупомянутое изобретение было довольно подробно описано с помощью иллюстраций и примеров с целью ясности понимания, очевидно, что на практике могут быть осуществлены определенные изменения и модификации в пределах объема прилагаемой формулы изобретения.
--->
СПИСОК ПОСЛЕДОВАТЕЛЬНОСТЕЙ
<110> AgBiome, Inc.
Parks, Jessica
Roberts, Kira Bulazel
Thayer, Rebecca E.
<120> ПЕСТИЦИДНЫЕ ГЕНЫ И СПОСОБЫ ИХ ПРИМЕНЕНИЯ
<130> 098699-1005793
<150> US 62/151,156
<151> 2015-04-22
<160> 398
<170> PatentIn version 3.5
<210> 1
<211> 1231
<212> БЕЛОК
<213> Bacillus sp.
<400> 1
Met Asn Gln Asn Asp Asn Asn Asn Glu Phe Glu Ile Met Asp Asn Gly
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Ser Met Lys Ser Gln Leu Arg Tyr Pro Leu Ala Asn Thr Ser Asp Phe
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Glu Trp Asn Ser Ser Val Gly Arg Lys Tyr Glu Ile Met Gln Gln Ala
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Asn Val Asp Gly Ala Thr Gly Ala Ala Leu Gly Ile Ile Ser Asn Thr
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Leu Gly Ala Asn Lys Gln Ala Leu Asp Lys Ala Ser Gly Ile Ile Gly
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Leu Ile Phe Lys Ile Leu Trp Lys Lys Phe His Asp Gln Ser Gln Trp
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Glu Glu Phe Met Asn Ser Val Glu Tyr Leu Ile Asn Gln Lys Ile Thr
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Glu Leu Val Arg Ser Gln Ala Val Ala Asp Leu Leu Gly Val Gln Glu
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Ala Leu Glu Leu Tyr Gln Glu Ala Ala Asn Glu Trp Asn Glu Asn Pro
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Asp Asn Pro Asn Ala Gln Glu Arg Ile Arg Arg Gln Phe Thr Ala Thr
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Asn Thr Thr Ile Ala Asn Ser Met Pro Ser Phe Gln Val Thr Gly Phe
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Glu Val Pro Leu Leu Ala Val Phe Ala Gln Ala Ala Asn Leu Tyr Leu
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Leu Leu Leu Arg Asp Ala Val Lys Phe Gly Lys Gly Trp Gly Leu Thr
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Ser Gln Glu Ile Glu Asp Leu Tyr Asn Arg Leu Gln Arg Asn Thr Ala
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Asn Tyr Thr Asp Tyr Cys Val Thr Thr Tyr Asp Lys Gly Leu Lys Gln
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Ala Tyr Asp Leu Val Pro Asn Pro Lys Asp Tyr Asn Lys Tyr Pro Tyr
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Leu Asn Pro Tyr Ser Asn Asp Pro Ile Tyr Gly Lys Tyr Tyr Thr Ala
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Pro Val Asp Trp Asn Leu Phe Asn Asp Tyr Arg Arg Asn Met Thr Leu
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Met Val Leu Asp Ile Val Ala Val Trp Pro Thr Tyr Asn Pro Arg Val
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Tyr Asp Asn Pro Asn Gly Val Gln Ile Glu Leu Ser Arg Glu Val Tyr
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Ser Thr Val Tyr Gly Arg Gly Gly Ser Asn Asn Ser Ser Phe Asp Ala
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Ile Glu Ser Gln Ile Val Arg Pro Pro His Leu Val Thr Glu Leu Thr
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Thr Leu Lys Ile Glu Gln Gly Ala Thr Leu Asp Tyr Glu Gln Ile Gln
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Tyr Pro Lys Tyr Met Lys Val Thr Asn Thr Leu His Tyr Thr Gly Ser
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Asn Thr Phe Glu Lys Ser Ser Ser Ala Pro Leu Ile Arg Pro Ile Thr
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Gln Leu Glu Val Pro Tyr Arg Phe Ala Phe Tyr Asn Arg Glu Asn Ala
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Asp Gly Ile Pro Lys Ala Glu Gly Ser Asn Gln Asn Ser His His Leu
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Thr Tyr Pro Thr Glu Leu Leu Gly Glu Trp Gly Phe Gly Trp Leu His
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Asn Ser Leu Thr Pro Glu Asn Lys Ile Val Pro Asp Lys Ile Thr Gln
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Ile Pro Ala Val Lys Gly Lys Ser Leu Ala His Gly Ala Gln Val Val
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Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu Val Ser Leu Ser Ser Gln
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Leu Ser Gln Leu Gln Leu Leu Leu Asn Leu Ile Ser Pro Ile Pro Thr
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Pro Glu Leu Val Gly His Tyr Ile Arg Ile Arg Tyr Ala Ser Ser Ala
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Asn Thr Gln Leu Tyr Ile Ser Cys Gly Ser Arg Val Gly Tyr Tyr Asp
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Val Lys Ala Thr Tyr Ser Gly Gly Thr Leu Thr Tyr Gln Ser Phe Gly
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Tyr Met Asp Ala Gly Trp Leu Tyr Met Val Gly Gly Phe Asn Phe Ile
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Ala Ile Ile Arg Asn Gln Gly Asn Thr Pro Ile Ile Ile Asp Lys Ile
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Glu Phe Ile Pro Ile Lys Gly Ser Leu Glu Glu Tyr Gln Ala Asp Gln
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Glu Leu Glu Lys Ala Arg Thr Ala Val Asn Ala Leu Phe Thr Ser Asp
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Ala Lys Asn Asp Leu Lys Leu Asn Ile Thr Asp Tyr Ala Val Asp Gln
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Ala Ala Asn Leu Val Glu Cys Val Ser Glu Asp Phe His Ala Gln Glu
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Lys Met Ile Leu Leu Asp Gln Val Lys Phe Ala Lys Arg Leu Ser Gln
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Ala Arg Asn Leu Leu Asn Tyr Gly Asp Phe Glu Ser Ser Asp Trp Ser
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Gly Glu Asn Gly Trp Arg Thr Ser Gln His Val His Val Gly Ser Gly
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Asn Pro Ile Phe Lys Gly Arg Tyr Leu His Met Pro Gly Ala Met Ser
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Asp Glu Ser Lys Leu Lys Ser Tyr Thr Arg Tyr Leu Val Arg Gly Phe
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Val Gly Asn Ser Lys Asp Leu Glu Leu Leu Val Glu Arg Tyr Gly Lys
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Pro Ile Asn Glu Cys Gly Asp Phe Asp Arg Cys Arg Pro Val Ser Tyr
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Gln Thr Arg Ala Ala His Thr Cys Thr Cys Lys Asp Thr Ala Ile Ala
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Met Tyr Thr Asn Val Ser Thr Asp Ser Asp Met Tyr Thr Asn Gly Phe
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His Ala His Lys Ser Cys Gly Cys Lys Asn Asn Asp Met Tyr Gln Asn
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Glu Ser His Pro His Lys Ser Cys Gly Cys Lys Asp Pro His Val Phe
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Gln Asn Leu Phe Thr Asn Ala Gln Gln His Arg Leu Lys Phe Glu
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Thr Leu Phe Ser Gln Ile Val His Ala Glu Met Leu Val Gln Gln
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Val Pro Gly Met Asn Phe Asp Ile Val Gln Gln Phe Leu Val Gly
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Asp Arg Gly Tyr Val Leu Arg Val Thr Ala Arg Lys Glu Gly Gly
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Gly Lys Gly Thr Val Thr Met Asn Asp Cys Ala Ala Tyr Thr Glu
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Thr Met Thr Ser Gly Thr Val Ser Gly Phe Val Thr Lys Thr Leu
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Glu Ile Phe Pro Asp Thr Asp Arg Ile Arg Ile Asp Ile Gly Glu
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Thr Glu Gly Met Phe Arg Ile Glu Ser Val Glu Leu Ile Cys Met
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<210> 2
<211> 645
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 2
Met Asn Gln Asn Asp Asn Asn Asn Glu Phe Glu Ile Met Asp Asn Gly
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Ser Met Lys Ser Gln Leu Arg Tyr Pro Leu Ala Asn Thr Ser Asp Phe
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Glu Trp Asn Ser Ser Val Gly Arg Lys Tyr Glu Ile Met Gln Gln Ala
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Asn Val Asp Gly Ala Thr Gly Ala Ala Leu Gly Ile Ile Ser Asn Thr
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Leu Gly Ala Asn Lys Gln Ala Leu Asp Lys Ala Ser Gly Ile Ile Gly
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Leu Ile Phe Lys Ile Leu Trp Lys Lys Phe His Asp Gln Ser Gln Trp
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Glu Glu Phe Met Asn Ser Val Glu Tyr Leu Ile Asn Gln Lys Ile Thr
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Glu Leu Val Arg Ser Gln Ala Val Ala Asp Leu Leu Gly Val Gln Glu
115 120 125
Ala Leu Glu Leu Tyr Gln Glu Ala Ala Asn Glu Trp Asn Glu Asn Pro
130 135 140
Asp Asn Pro Asn Ala Gln Glu Arg Ile Arg Arg Gln Phe Thr Ala Thr
145 150 155 160
Asn Thr Thr Ile Ala Asn Ser Met Pro Ser Phe Gln Val Thr Gly Phe
165 170 175
Glu Val Pro Leu Leu Ala Val Phe Ala Gln Ala Ala Asn Leu Tyr Leu
180 185 190
Leu Leu Leu Arg Asp Ala Val Lys Phe Gly Lys Gly Trp Gly Leu Thr
195 200 205
Ser Gln Glu Ile Glu Asp Leu Tyr Asn Arg Leu Gln Arg Asn Thr Ala
210 215 220
Asn Tyr Thr Asp Tyr Cys Val Thr Thr Tyr Asp Lys Gly Leu Lys Gln
225 230 235 240
Ala Tyr Asp Leu Val Pro Asn Pro Lys Asp Tyr Asn Lys Tyr Pro Tyr
245 250 255
Leu Asn Pro Tyr Ser Asn Asp Pro Ile Tyr Gly Lys Tyr Tyr Thr Ala
260 265 270
Pro Val Asp Trp Asn Leu Phe Asn Asp Tyr Arg Arg Asn Met Thr Leu
275 280 285
Met Val Leu Asp Ile Val Ala Val Trp Pro Thr Tyr Asn Pro Arg Val
290 295 300
Tyr Asp Asn Pro Asn Gly Val Gln Ile Glu Leu Ser Arg Glu Val Tyr
305 310 315 320
Ser Thr Val Tyr Gly Arg Gly Gly Ser Asn Asn Ser Ser Phe Asp Ala
325 330 335
Ile Glu Ser Gln Ile Val Arg Pro Pro His Leu Val Thr Glu Leu Thr
340 345 350
Thr Leu Lys Ile Glu Gln Gly Ala Thr Leu Asp Tyr Glu Gln Ile Gln
355 360 365
Tyr Pro Lys Tyr Met Lys Val Thr Asn Thr Leu His Tyr Thr Gly Ser
370 375 380
Asn Thr Phe Glu Lys Ser Ser Ser Ala Pro Leu Ile Arg Pro Ile Thr
385 390 395 400
Lys Thr Tyr Thr Ile Pro Ala Asn Asn Ile Gly Asn Leu Ser Leu Ser
405 410 415
Gln Leu Glu Val Pro Tyr Arg Phe Ala Phe Tyr Asn Arg Glu Asn Ala
420 425 430
Leu Ile Ala Glu Val Gly Ala Glu Phe Pro Pro Asn Thr Val Thr Trp
435 440 445
Asp Gly Ile Pro Lys Ala Glu Gly Ser Asn Gln Asn Ser His His Leu
450 455 460
Ser Tyr Val Gly Ala Leu Asn Thr Gln Ser Ser Ala Gly Phe Pro Phe
465 470 475 480
Thr Tyr Pro Thr Glu Leu Leu Gly Glu Trp Gly Phe Gly Trp Leu His
485 490 495
Asn Ser Leu Thr Pro Glu Asn Lys Ile Val Pro Asp Lys Ile Thr Gln
500 505 510
Ile Pro Ala Val Lys Gly Lys Ser Leu Ala His Gly Ala Gln Val Val
515 520 525
Lys Gly Pro Gly Ser Thr Gly Gly Asp Leu Val Ser Leu Ser Ser Gln
530 535 540
Leu Ser Gln Leu Gln Leu Leu Leu Asn Leu Ile Ser Pro Ile Pro Thr
545 550 555 560
Pro Glu Leu Val Gly His Tyr Ile Arg Ile Arg Tyr Ala Ser Ser Ala
565 570 575
Asn Thr Gln Leu Tyr Ile Ser Cys Gly Ser Arg Val Gly Tyr Tyr Asp
580 585 590
Val Lys Ala Thr Tyr Ser Gly Gly Thr Leu Thr Tyr Gln Ser Phe Gly
595 600 605
Tyr Met Asp Ala Gly Trp Leu Tyr Met Val Gly Gly Phe Asn Phe Ile
610 615 620
Ala Ile Ile Arg Asn Gln Gly Asn Thr Pro Ile Ile Ile Asp Lys Ile
625 630 635 640
Glu Phe Ile Pro Ile
645
<210> 3
<211> 1238
<212> БЕЛОК
<213> Bacillus sp.
<400> 3
Met Gly Gly Leu Leu Met Asn Gln Asn Tyr Asn Asn Asn Glu Tyr Glu
1 5 10 15
Ile Met Asp Asn Gly Val Gln Cys Cys Thr Pro Arg Tyr Pro Leu Ala
20 25 30
Gln Ala Pro Gly Ser Glu Leu Gln Ser Met Asn Tyr Gln Asp Val Thr
35 40 45
Glu Thr Ser Ile Gly Arg Glu Tyr Gln Ala Val Gln Gln Val Asn Val
50 55 60
Gly Lys Ala Val Ser Ala Ala Leu Gly Ile Leu Thr Thr Ile Leu Lys
65 70 75 80
Ala Ala Asn Pro Ile Leu Gly Thr Ala Ala Gly Val Ile Ser Ser Ile
85 90 95
Phe Gly Phe Leu Trp Lys Arg Phe Gly Thr Asp Pro Gln Ser Gln Trp
100 105 110
Lys Gln Phe Met Glu Ala Val Glu Tyr Leu Val Ser Gln Lys Ile Thr
115 120 125
Asp Ala Val Arg Ser Lys Ala Val Ser Glu Leu Glu Gly Val Gln Arg
130 135 140
Ala Val Glu Leu Tyr Gln Glu Ala Ala Asn Ala Trp Asn Met Asn Pro
145 150 155 160
Asp Asp Ala Ala Ala Lys Glu Arg Ile Arg Arg Gln Tyr Thr Ser Thr
165 170 175
Asn Thr Val Ile Glu Phe Ala Met Pro Ser Phe Arg Val Gly Gly Phe
180 185 190
Glu Val Pro Leu Leu Thr Val Tyr Ala Gln Ala Ala Asn Leu His Leu
195 200 205
Leu Leu Leu Arg Asp Ala Val Lys Phe Gly Ala Gly Trp Gly Phe Ser
210 215 220
Pro Thr Glu Val Glu Asp Asn Tyr Thr Arg Leu Gln Ala Arg Thr Ala
225 230 235 240
Glu Tyr Thr Asn His Cys Thr Asn Thr Tyr Asp Lys Gly Leu Lys Gln
245 250 255
Ala Tyr Asp Leu Ala Pro Asn Pro Thr Asp Tyr Asn Lys Tyr Pro Tyr
260 265 270
Leu Asn Pro Tyr Ser Lys Asp Pro Ile Tyr Gly Lys Tyr Tyr Thr Ala
275 280 285
Pro Val Asp Trp Asn Leu Phe Asn Asp Phe Arg Arg Asp Met Thr Leu
290 295 300
Met Val Leu Asp Ile Val Ala Val Trp Pro Thr Tyr Asn Pro Arg Leu
305 310 315 320
Tyr Asn Asn Pro Asn Gly Val Gln Ile Gln Leu Ser Arg Glu Val Tyr
325 330 335
Ser Thr Val Tyr Gly Arg Ala Trp Ser Asn Asn Ser Thr Val Asp Ala
340 345 350
Ile Glu Ser Thr Leu Val Arg Pro Pro His Leu Val Thr Glu Leu Thr
355 360 365
Lys Leu Thr Phe Asp Glu Arg Asn Leu Tyr Glu Ala Glu Thr Val Pro
370 375 380
Val Ala Phe Arg Arg Val Ala Asn Thr Leu His Asn Val Gly Ser Ser
385 390 395 400
Thr Thr Trp Glu Gln Ser Phe Ser Ala Thr Ser Val Gly Ser Leu Lys
405 410 415
Ala Val His Asn Val Ala Ala Thr Asp Ile Gly Asn Leu Ala Leu Ser
420 425 430
Leu Gly Ala Val Pro Leu Gly Phe Ser Phe Tyr Asn Lys Asn Asp Gln
435 440 445
His Leu Thr Thr Val Gly Tyr Ser Gly Gly Trp Trp Asn Gly Ile Pro
450 455 460
Lys Asp Glu Gly Ser Asn Gln Asn Ser His His Leu Ser Tyr Val Ala
465 470 475 480
Ala Leu Glu Thr Gln Ser Thr Ala Gly Trp Trp Pro Tyr Thr Tyr Pro
485 490 495
Val Pro Leu Leu Gly Glu Trp Gly Phe Gly Trp Leu His Asn Ser Leu
500 505 510
Thr Pro Thr Asn Glu Ile Leu Ser Asp Lys Ile Thr Gln Ile Pro Ala
515 520 525
Val Lys Ala Phe Asn Ile Gln Gly Asn Gly Lys Val Thr Lys Gly Pro
530 535 540
Gly Ser Thr Gly Gly Asp Leu Ile Ala Leu Pro Ala Pro Ser Gly Gln
545 550 555 560
Ile Asn Ile Tyr Leu Lys Glu Thr Ser Thr Lys Asn Tyr Arg Val Arg
565 570 575
Leu Arg Tyr Ala Ala Thr Thr Asp Gly Gln Leu Arg Val Ala Leu Val
580 585 590
Arg Asn Gly Gly Ala Val Arg Trp Phe Glu Asn Ile Lys Tyr Val Thr
595 600 605
Thr Gly Ala Ser Glu Asn Ser Leu Ala Tyr Asn Gln Phe Lys Tyr Val
610 615 620
Asp Ile Phe Thr Ser Asp Phe Ser Asn Ile Asn Ser Leu Val Phe Ile
625 630 635 640
Asn Gln Ser Gly Gly Thr Ile Leu Ile Asp Gln Ile Glu Phe Ile Pro
645 650 655
Ile Glu Gly Ser Leu Glu Val Phe Glu Ala Glu Gln Asp Leu Glu Asn
660 665 670
Ala Arg Lys Ala Val Asn Ala Leu Phe Thr Gly Ala Ala Lys Asp Val
675 680 685
Leu Lys Leu Asn Val Thr Asp Tyr Ala Val Asp Gln Ala Ala Asn Leu
690 695 700
Val Glu Cys Val Ser Asp Glu Met His Pro Lys Glu Lys Met Ile Leu
705 710 715 720
Leu Asp Gln Val Lys Phe Ala Lys Arg Leu Ser Gln Thr Arg Asn Leu
725 730 735
Leu Asn Tyr Gly Asp Phe Glu Ser Asp Asp Trp Ser Gly Ala Asn Gly
740 745 750
Trp Lys Thr Ser Asn His Val His Val Ala Ala Asp Asn Pro Ile Phe
755 760 765
Lys Gly Arg Tyr Leu His Met Pro Gly Ala Thr Ser Ser Gln Leu Ser
770 775 780
Ser Asn Val Tyr Pro Thr Tyr Ala Tyr Gln Lys Val Asp Glu Ser Lys
785 790 795 800
Leu Lys Ser Tyr Thr Arg Tyr Leu Val Arg Gly Phe Ile Gly Asn Ser
805 810 815
Lys Asp Leu Glu Leu Leu Val Glu Arg Tyr Gly Lys Asp Val Tyr Val
820 825 830
Glu Met Asp Val Pro Asn Asp Ile Gln Tyr Ala Leu Pro Met Asn Glu
835 840 845
Cys Gly Gly Phe Asp Arg Cys Gly His Val Ser Tyr Gln Ala Ala Ser
850 855 860
His His Thr Cys Thr Cys Lys Asn Thr Ala Gln Met Asp Thr Asp Cys
865 870 875 880
Gln Cys Lys Asp Lys Val Asn Arg Thr Ala Pro Gly Val Tyr Thr Asn
885 890 895
Thr Val Pro Gly Ser Ala Met Tyr Thr Asn Gly Tyr His Ser His Lys
900 905 910
Ser Cys Gly Cys Lys Asp Pro His Val Phe Ser Tyr His Ile Asp Thr
915 920 925
Gly Cys Val Asp Gln Glu Glu Asn Leu Gly Leu Trp Phe Ala Leu Lys
930 935 940
Ile Ala Ser Glu Asn Gly Val Ala Lys Ile Asp Asn Leu Glu Ile Ile
945 950 955 960
Glu Ala Gln Pro Leu Thr Gly Glu Ala Leu Ala Arg Val Lys Lys Arg
965 970 975
Glu His Lys Trp Lys Gln Glu Met Ala Gln Lys Arg Leu His Thr Glu
980 985 990
Lys Ala Val Gln Ala Ala Arg Asp Ala Ile Gln Arg Leu Phe Thr Ser
995 1000 1005
Pro Lys Leu Asn Arg Leu Lys Phe Glu Thr Leu Leu Gln Gln Ile
1010 1015 1020
Leu Asn Ala Asp Ala Leu Val Gln Lys Ile Ser Tyr Val Tyr His
1025 1030 1035
Ser Phe Leu Ile Gly Ala Met Pro Thr Val Pro Gly Met Asn Asn
1040 1045 1050
Glu Ile Tyr Gln Lys Leu Ser Ala Ala Ile Ala Gln Ala Arg Ala
1055 1060 1065
Leu Tyr Glu Gln Arg Asn Leu Val Arg Asn Gly Thr Phe Ser Ser
1070 1075 1080
Gly Thr Gly Ser Trp His Val Ser Asp Gly Val Glu Val Gln Pro
1085 1090 1095
Leu Gln Asn Thr Phe Val Leu Val Leu Ser Glu Trp Ser Asp Glu
1100 1105 1110
Ala Ser Gln Asp Arg Arg Ile Asp Pro Glu Arg Gly Tyr Val Leu
1115 1120 1125
Arg Val Thr Ala Arg Lys Glu Gly Ala Gly Lys Gly Thr Val Thr
1130 1135 1140
Ile Ser Asp Cys Ala Asp Tyr Thr Glu Lys Leu Thr Phe Thr Ser
1145 1150 1155
Cys Asp Tyr Asn Thr Val Gly Ser Gln Thr Met Thr Ser Gly Thr
1160 1165 1170
Leu Ser Gly Phe Val Thr Lys Thr Leu Glu Ile Phe Pro Asp Thr
1175 1180 1185
Asp Arg Ile Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr Phe Lys
1190 1195 1200
Ile Glu Ser Val Glu Leu Ile Cys Met Glu Gln Met Glu Asp His
1205 1210 1215
Met Tyr Asp Met Ala Gly Asn Val Glu Ala Ser Ile Pro Pro Leu
1220 1225 1230
Val Pro Pro Met Asn
1235
<210> 4
<211> 657
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 4
Met Gly Gly Leu Leu Met Asn Gln Asn Tyr Asn Asn Asn Glu Tyr Glu
1 5 10 15
Ile Met Asp Asn Gly Val Gln Cys Cys Thr Pro Arg Tyr Pro Leu Ala
20 25 30
Gln Ala Pro Gly Ser Glu Leu Gln Ser Met Asn Tyr Gln Asp Val Thr
35 40 45
Glu Thr Ser Ile Gly Arg Glu Tyr Gln Ala Val Gln Gln Val Asn Val
50 55 60
Gly Lys Ala Val Ser Ala Ala Leu Gly Ile Leu Thr Thr Ile Leu Lys
65 70 75 80
Ala Ala Asn Pro Ile Leu Gly Thr Ala Ala Gly Val Ile Ser Ser Ile
85 90 95
Phe Gly Phe Leu Trp Lys Arg Phe Gly Thr Asp Pro Gln Ser Gln Trp
100 105 110
Lys Gln Phe Met Glu Ala Val Glu Tyr Leu Val Ser Gln Lys Ile Thr
115 120 125
Asp Ala Val Arg Ser Lys Ala Val Ser Glu Leu Glu Gly Val Gln Arg
130 135 140
Ala Val Glu Leu Tyr Gln Glu Ala Ala Asn Ala Trp Asn Met Asn Pro
145 150 155 160
Asp Asp Ala Ala Ala Lys Glu Arg Ile Arg Arg Gln Tyr Thr Ser Thr
165 170 175
Asn Thr Val Ile Glu Phe Ala Met Pro Ser Phe Arg Val Gly Gly Phe
180 185 190
Glu Val Pro Leu Leu Thr Val Tyr Ala Gln Ala Ala Asn Leu His Leu
195 200 205
Leu Leu Leu Arg Asp Ala Val Lys Phe Gly Ala Gly Trp Gly Phe Ser
210 215 220
Pro Thr Glu Val Glu Asp Asn Tyr Thr Arg Leu Gln Ala Arg Thr Ala
225 230 235 240
Glu Tyr Thr Asn His Cys Thr Asn Thr Tyr Asp Lys Gly Leu Lys Gln
245 250 255
Ala Tyr Asp Leu Ala Pro Asn Pro Thr Asp Tyr Asn Lys Tyr Pro Tyr
260 265 270
Leu Asn Pro Tyr Ser Lys Asp Pro Ile Tyr Gly Lys Tyr Tyr Thr Ala
275 280 285
Pro Val Asp Trp Asn Leu Phe Asn Asp Phe Arg Arg Asp Met Thr Leu
290 295 300
Met Val Leu Asp Ile Val Ala Val Trp Pro Thr Tyr Asn Pro Arg Leu
305 310 315 320
Tyr Asn Asn Pro Asn Gly Val Gln Ile Gln Leu Ser Arg Glu Val Tyr
325 330 335
Ser Thr Val Tyr Gly Arg Ala Trp Ser Asn Asn Ser Thr Val Asp Ala
340 345 350
Ile Glu Ser Thr Leu Val Arg Pro Pro His Leu Val Thr Glu Leu Thr
355 360 365
Lys Leu Thr Phe Asp Glu Arg Asn Leu Tyr Glu Ala Glu Thr Val Pro
370 375 380
Val Ala Phe Arg Arg Val Ala Asn Thr Leu His Asn Val Gly Ser Ser
385 390 395 400
Thr Thr Trp Glu Gln Ser Phe Ser Ala Thr Ser Val Gly Ser Leu Lys
405 410 415
Ala Val His Asn Val Ala Ala Thr Asp Ile Gly Asn Leu Ala Leu Ser
420 425 430
Leu Gly Ala Val Pro Leu Gly Phe Ser Phe Tyr Asn Lys Asn Asp Gln
435 440 445
His Leu Thr Thr Val Gly Tyr Ser Gly Gly Trp Trp Asn Gly Ile Pro
450 455 460
Lys Asp Glu Gly Ser Asn Gln Asn Ser His His Leu Ser Tyr Val Ala
465 470 475 480
Ala Leu Glu Thr Gln Ser Thr Ala Gly Trp Trp Pro Tyr Thr Tyr Pro
485 490 495
Val Pro Leu Leu Gly Glu Trp Gly Phe Gly Trp Leu His Asn Ser Leu
500 505 510
Thr Pro Thr Asn Glu Ile Leu Ser Asp Lys Ile Thr Gln Ile Pro Ala
515 520 525
Val Lys Ala Phe Asn Ile Gln Gly Asn Gly Lys Val Thr Lys Gly Pro
530 535 540
Gly Ser Thr Gly Gly Asp Leu Ile Ala Leu Pro Ala Pro Ser Gly Gln
545 550 555 560
Ile Asn Ile Tyr Leu Lys Glu Thr Ser Thr Lys Asn Tyr Arg Val Arg
565 570 575
Leu Arg Tyr Ala Ala Thr Thr Asp Gly Gln Leu Arg Val Ala Leu Val
580 585 590
Arg Asn Gly Gly Ala Val Arg Trp Phe Glu Asn Ile Lys Tyr Val Thr
595 600 605
Thr Gly Ala Ser Glu Asn Ser Leu Ala Tyr Asn Gln Phe Lys Tyr Val
610 615 620
Asp Ile Phe Thr Ser Asp Phe Ser Asn Ile Asn Ser Leu Val Phe Ile
625 630 635 640
Asn Gln Ser Gly Gly Thr Ile Leu Ile Asp Gln Ile Glu Phe Ile Pro
645 650 655
Ile
<210> 5
<211> 357
<212> БЕЛОК
<213> Bacillus sp.
<400> 5
Leu Phe Met Lys Ile Lys Lys Val Ile Pro Ser Ile Ile Ala Phe Ser
1 5 10 15
Thr Leu Leu Thr Ile Ala Pro Val Ser Ser Phe Ala Ala Glu His Asp
20 25 30
Ala Leu Lys Asn Ser Lys Leu Ser Thr Ala Glu Arg Ala Ser Tyr Ile
35 40 45
Asp Val Val Gln Asp Arg Met Lys Thr Arg Asp Leu Glu Ser Arg Leu
50 55 60
Phe Asn Thr Pro Val Asn Ile Gln Glu Gln Ile Ile Asp Gly Trp Phe
65 70 75 80
Leu Ala Arg Phe Trp Ile Leu Lys Asp Gln Asn Asn Ser His Gln Thr
85 90 95
Asn Arg Phe Ile Ser Trp Phe Lys Asn Asn Leu Ala Ser Ala Lys Gly
100 105 110
Tyr Asp Arg Ile Ala Glu Gln Ile Gly Leu Lys Ile Glu Ala Arg Glu
115 120 125
Asp Met Asp Ile Ser Asn Val Gln Tyr Thr Ser Lys Ile Gly Glu Thr
130 135 140
Ile Tyr Gln Gly Val Ser Glu Leu Thr Asn Thr Phe Asn Ile Thr Gln
145 150 155 160
Lys Leu Lys Thr Asp Ser Phe Gln Arg Asp Tyr Thr Lys Ser Gln Thr
165 170 175
Thr Ser Ile Thr Asn Gly Leu Gln Leu Gly Phe Lys Val Ala Ala Lys
180 185 190
Gly Val Val Pro Leu Ala Gly Ala Asp Phe Glu Ala Ser Val Ala Tyr
195 200 205
Asn Leu Ser Gly Thr Ser Ser Glu Thr Ser Thr Ile Ser Asp Lys Phe
210 215 220
Ser Val Pro Ser Gln Glu Val Thr Leu Pro Pro Gly His Lys Ala Ile
225 230 235 240
Ile Lys His Asp Leu Arg Lys Met Ile Tyr Ser Gly Thr Asn Asp Val
245 250 255
Lys Gly Asp Leu Asn Val Thr Phe Asp Asp Lys Glu Ile Ile Gln Lys
260 265 270
Phe Ile His Ser Lys Tyr Lys Ser Ile Asn Leu Ser Asp Ile Arg Ala
275 280 285
Thr Met Ile Ala Ile Asp Lys Trp Asn Lys Val Thr Pro Thr Asp Phe
290 295 300
Tyr Asn Leu Leu Gly Lys Lys Asn Tyr Ile Lys Asp Asn Glu Thr Leu
305 310 315 320
Tyr Ile Asp Thr Pro Ala Lys Phe Asn Phe Asn Gly Ala Thr Pro Tyr
325 330 335
Tyr Arg Ala Thr Phe Ile Gln Tyr Asp Glu Asn Gly Lys Leu Val Lys
340 345 350
Thr Gln Lys Ile Asn
355
<210> 6
<211> 330
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 6
Met Ala Glu His Asp Ala Leu Lys Asn Ser Lys Leu Ser Thr Ala Glu
1 5 10 15
Arg Ala Ser Tyr Ile Asp Val Val Gln Asp Arg Met Lys Thr Arg Asp
20 25 30
Leu Glu Ser Arg Leu Phe Asn Thr Pro Val Asn Ile Gln Glu Gln Ile
35 40 45
Ile Asp Gly Trp Phe Leu Ala Arg Phe Trp Ile Leu Lys Asp Gln Asn
50 55 60
Asn Ser His Gln Thr Asn Arg Phe Ile Ser Trp Phe Lys Asn Asn Leu
65 70 75 80
Ala Ser Ala Lys Gly Tyr Asp Arg Ile Ala Glu Gln Ile Gly Leu Lys
85 90 95
Ile Glu Ala Arg Glu Asp Met Asp Ile Ser Asn Val Gln Tyr Thr Ser
100 105 110
Lys Ile Gly Glu Thr Ile Tyr Gln Gly Val Ser Glu Leu Thr Asn Thr
115 120 125
Phe Asn Ile Thr Gln Lys Leu Lys Thr Asp Ser Phe Gln Arg Asp Tyr
130 135 140
Thr Lys Ser Gln Thr Thr Ser Ile Thr Asn Gly Leu Gln Leu Gly Phe
145 150 155 160
Lys Val Ala Ala Lys Gly Val Val Pro Leu Ala Gly Ala Asp Phe Glu
165 170 175
Ala Ser Val Ala Tyr Asn Leu Ser Gly Thr Ser Ser Glu Thr Ser Thr
180 185 190
Ile Ser Asp Lys Phe Ser Val Pro Ser Gln Glu Val Thr Leu Pro Pro
195 200 205
Gly His Lys Ala Ile Ile Lys His Asp Leu Arg Lys Met Ile Tyr Ser
210 215 220
Gly Thr Asn Asp Val Lys Gly Asp Leu Asn Val Thr Phe Asp Asp Lys
225 230 235 240
Glu Ile Ile Gln Lys Phe Ile His Ser Lys Tyr Lys Ser Ile Asn Leu
245 250 255
Ser Asp Ile Arg Ala Thr Met Ile Ala Ile Asp Lys Trp Asn Lys Val
260 265 270
Thr Pro Thr Asp Phe Tyr Asn Leu Leu Gly Lys Lys Asn Tyr Ile Lys
275 280 285
Asp Asn Glu Thr Leu Tyr Ile Asp Thr Pro Ala Lys Phe Asn Phe Asn
290 295 300
Gly Ala Thr Pro Tyr Tyr Arg Ala Thr Phe Ile Gln Tyr Asp Glu Asn
305 310 315 320
Gly Lys Leu Val Lys Thr Gln Lys Ile Asn
325 330
<210> 7
<211> 298
<212> БЕЛОК
<213> Bacillus sp.
<400> 7
Met Ala Ile Val Asp Leu Asp Ala Tyr Leu Leu Ala Leu Ser Lys Lys
1 5 10 15
Val Leu Ala Ala Pro Phe Asp Pro Phe Ile Ser Arg Val Asn Thr Ser
20 25 30
Arg Leu Ala Val Lys Asn Ala Glu Ser Tyr Gly Phe Glu Ile Lys Glu
35 40 45
Ser Lys Pro Gln Gly Thr Met Phe Ile Gly Glu Ser Ile Leu Asn Asn
50 55 60
Arg Thr Asn Glu Thr Gln Thr Leu His Ser Asp Ser Phe Thr Lys Thr
65 70 75 80
Ile Thr Asp Ser Val Thr Leu Ser Val Thr Thr Gly Ile Thr Thr Gly
85 90 95
Val Asn Ile Asn Ile Gly Gly Lys Ile Phe Gly Met Gly Val Glu Thr
100 105 110
Ser Met Ser Phe Glu Val Ser Thr Ser Thr Thr Asn Glu Gln Thr Asn
115 120 125
Glu Glu Ser Val Ala Tyr Thr Val Pro Ser Gln Thr Val Val Val Pro
130 135 140
Ala Gln Thr Thr Tyr Tyr Val His Thr Ala Leu Glu Arg Asn Gln Leu
145 150 155 160
Glu Gly Ile Ile Arg Leu Arg Val Asp Leu Ser Gly Glu Phe Ser Ala
165 170 175
Met Ile Lys Val Gln Asp Leu Glu Val Pro Ile Tyr Thr Gly Asn Ile
180 185 190
Tyr Asp Phe Val Asn Gln Tyr Gln Ser Leu His Pro Leu Pro Ser Gly
195 200 205
Ile Ser Leu Asn His Asn Thr Lys Ser Ile His Phe Glu Gly Ile Ala
210 215 220
Glu Tyr Leu Tyr Gly Thr Gly Thr Lys Phe His Val Thr Val Thr Asp
225 230 235 240
Lys Pro Ser Ser Gln Gly Thr Gln Glu His Lys Ser Phe Asp Ala Lys
245 250 255
Thr Gly Leu Gly Thr Tyr Pro Ile Gly Leu Asn Gly Lys Lys Leu Ser
260 265 270
Leu Glu Leu Asn Asp Leu Lys Glu His Met Asn Ala Lys Asp Phe Glu
275 280 285
Lys Leu Lys Glu Leu Gln Asn Glu Val Val
290 295
<210> 8
<211> 556
<212> БЕЛОК
<213> Bacillus sp.
<400> 8
Leu Cys Tyr Met Asn Lys Gln Asp Thr Gln Ile Ala Glu Leu Pro Val
1 5 10 15
Ile Asp Glu Phe Asn Gly Ile Ile Gly Phe Ala Gln Asn Ile Gly Gly
20 25 30
Ile Met Asn Met Ile Ala Gly Thr Pro Ser Val Asp Asp Met Thr Leu
35 40 45
Asp Gln Leu Val Thr Asn Tyr Asp Ala Leu Val Asp Ile Gln Thr Ser
50 55 60
Ile Ala Gly Ile Asn Gln Gln Leu Ala Glu Val Ile Lys Asn Gly Ser
65 70 75 80
Glu Asp Thr Asp Leu Ile Lys Gln Leu Ile Asp Ile Asn Asn Glu Gln
85 90 95
Ser Ala Glu Leu Asp Glu Ile Asn Gly Glu Ile Gln Asp Ile Asp Asn
100 105 110
Met Leu Asn Thr Tyr Leu Pro Glu Ile Val Asn Met Leu Glu Gly Ile
115 120 125
Ser Gln Gln Leu Val Gly Ile Ser Gln Gln Ile Asn Gln Met Met Gln
130 135 140
Met Leu Asn Ala Ile Ser Lys Gln Leu Asn Lys Ile Ala Ala Asn Val
145 150 155 160
Ala Leu Asn Ser Thr Ile Ala Glu Ile Glu Pro His Tyr Gln Lys Ile
165 170 175
Lys Tyr Val Ile Thr Gln Phe Asn Glu Leu Asn Asp Glu Leu Val Thr
180 185 190
Thr Tyr Ser Ser Gln Ile Asn Asn Asn Glu Leu His Leu Gly Ala Asp
195 200 205
Asp Ile Asp Thr Gly Ala Leu Ala Ala Leu Ile Ala Trp Ala Lys Met
210 215 220
Val Val Ala Ile Asp Leu Asn Asp Leu Arg Leu Ser Leu Asp Ala Met
225 230 235 240
Asn Asp Ala Ile Leu Gly Asn Asn Leu Tyr Gly Asn Ser Ala Leu Val
245 250 255
Ser Thr Ala Asp Phe Tyr Thr Ala Asn Thr Thr Tyr Ala Gln Ser Ile
260 265 270
Tyr Thr Ser Met Tyr Ser Tyr Leu Ser Thr Leu Cys Gly Leu Gln Thr
275 280 285
Asn Ala Tyr Met Leu Val Asp Thr Ala Arg Lys Ile Leu Ser Leu Pro
290 295 300
Glu Glu Asp Asn Gln Asp Leu Met Asn Ser Arg Ile Ser Gln Gln Lys
305 310 315 320
Lys Leu Phe Thr Ser Leu Ser Ser Gly Phe Ser Asn Val Phe Tyr Ser
325 330 335
Thr Glu Ser Ile Asp Met Asp Pro Gly Ala Ser Ala Pro Ile Asn Gln
340 345 350
Ser Tyr Glu Ile Glu Leu Val Pro Pro Ala Gly Asn Ala Leu Leu Gly
355 360 365
Phe Asn Met Thr Ser Glu Pro Asp Ser Asn Gly Asp Val Gln Asn Gly
370 375 380
Lys Leu His Val Glu Tyr Tyr Tyr Gly Lys Leu Leu Thr Asn Tyr Gly
385 390 395 400
Val Asp Val Ala Ser Ile Asn Val Ala Thr Ala Glu Phe Ser Met Ala
405 410 415
Asn Tyr Leu Pro Ser Ser Gly Ile Tyr Asn Val Pro Asp Gly Gly Leu
420 425 430
Pro Thr Ile Asp Ser Asn Thr Ile Val Val Gly Phe Lys Leu Lys Ile
435 440 445
Thr Thr Asp Asn Met Gly Gln Ala Phe Met Val Pro Ser Val Val Thr
450 455 460
Ala Pro Tyr Asp Ser Thr Lys Asn Gly Val Gly Thr Thr Thr Lys Asp
465 470 475 480
Val Val Ile Pro Gln Ser Asp Asn Asn Ser Ile Thr Asn Gln Pro Met
485 490 495
Gly Ile Val Ser Asp Gln Phe Leu Ser Pro Ile Leu Asn Met Thr Met
500 505 510
Gly Lys Asp Glu Asn Gly Gln Tyr Cys Phe Met Val Glu Ser Ala Leu
515 520 525
Lys Asn Tyr Val Asn Ala Thr Asp Met Lys Asp Ala Asp Thr Asp Leu
530 535 540
Ala Val Ser Pro Tyr Tyr Ser Thr Asp Thr Ala Glu
545 550 555
<210> 9
<211> 553
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 9
Met Asn Lys Gln Asp Thr Gln Ile Ala Glu Leu Pro Val Ile Asp Glu
1 5 10 15
Phe Asn Gly Ile Ile Gly Phe Ala Gln Asn Ile Gly Gly Ile Met Asn
20 25 30
Met Ile Ala Gly Thr Pro Ser Val Asp Asp Met Thr Leu Asp Gln Leu
35 40 45
Val Thr Asn Tyr Asp Ala Leu Val Asp Ile Gln Thr Ser Ile Ala Gly
50 55 60
Ile Asn Gln Gln Leu Ala Glu Val Ile Lys Asn Gly Ser Glu Asp Thr
65 70 75 80
Asp Leu Ile Lys Gln Leu Ile Asp Ile Asn Asn Glu Gln Ser Ala Glu
85 90 95
Leu Asp Glu Ile Asn Gly Glu Ile Gln Asp Ile Asp Asn Met Leu Asn
100 105 110
Thr Tyr Leu Pro Glu Ile Val Asn Met Leu Glu Gly Ile Ser Gln Gln
115 120 125
Leu Val Gly Ile Ser Gln Gln Ile Asn Gln Met Met Gln Met Leu Asn
130 135 140
Ala Ile Ser Lys Gln Leu Asn Lys Ile Ala Ala Asn Val Ala Leu Asn
145 150 155 160
Ser Thr Ile Ala Glu Ile Glu Pro His Tyr Gln Lys Ile Lys Tyr Val
165 170 175
Ile Thr Gln Phe Asn Glu Leu Asn Asp Glu Leu Val Thr Thr Tyr Ser
180 185 190
Ser Gln Ile Asn Asn Asn Glu Leu His Leu Gly Ala Asp Asp Ile Asp
195 200 205
Thr Gly Ala Leu Ala Ala Leu Ile Ala Trp Ala Lys Met Val Val Ala
210 215 220
Ile Asp Leu Asn Asp Leu Arg Leu Ser Leu Asp Ala Met Asn Asp Ala
225 230 235 240
Ile Leu Gly Asn Asn Leu Tyr Gly Asn Ser Ala Leu Val Ser Thr Ala
245 250 255
Asp Phe Tyr Thr Ala Asn Thr Thr Tyr Ala Gln Ser Ile Tyr Thr Ser
260 265 270
Met Tyr Ser Tyr Leu Ser Thr Leu Cys Gly Leu Gln Thr Asn Ala Tyr
275 280 285
Met Leu Val Asp Thr Ala Arg Lys Ile Leu Ser Leu Pro Glu Glu Asp
290 295 300
Asn Gln Asp Leu Met Asn Ser Arg Ile Ser Gln Gln Lys Lys Leu Phe
305 310 315 320
Thr Ser Leu Ser Ser Gly Phe Ser Asn Val Phe Tyr Ser Thr Glu Ser
325 330 335
Ile Asp Met Asp Pro Gly Ala Ser Ala Pro Ile Asn Gln Ser Tyr Glu
340 345 350
Ile Glu Leu Val Pro Pro Ala Gly Asn Ala Leu Leu Gly Phe Asn Met
355 360 365
Thr Ser Glu Pro Asp Ser Asn Gly Asp Val Gln Asn Gly Lys Leu His
370 375 380
Val Glu Tyr Tyr Tyr Gly Lys Leu Leu Thr Asn Tyr Gly Val Asp Val
385 390 395 400
Ala Ser Ile Asn Val Ala Thr Ala Glu Phe Ser Met Ala Asn Tyr Leu
405 410 415
Pro Ser Ser Gly Ile Tyr Asn Val Pro Asp Gly Gly Leu Pro Thr Ile
420 425 430
Asp Ser Asn Thr Ile Val Val Gly Phe Lys Leu Lys Ile Thr Thr Asp
435 440 445
Asn Met Gly Gln Ala Phe Met Val Pro Ser Val Val Thr Ala Pro Tyr
450 455 460
Asp Ser Thr Lys Asn Gly Val Gly Thr Thr Thr Lys Asp Val Val Ile
465 470 475 480
Pro Gln Ser Asp Asn Asn Ser Ile Thr Asn Gln Pro Met Gly Ile Val
485 490 495
Ser Asp Gln Phe Leu Ser Pro Ile Leu Asn Met Thr Met Gly Lys Asp
500 505 510
Glu Asn Gly Gln Tyr Cys Phe Met Val Glu Ser Ala Leu Lys Asn Tyr
515 520 525
Val Asn Ala Thr Asp Met Lys Asp Ala Asp Thr Asp Leu Ala Val Ser
530 535 540
Pro Tyr Tyr Ser Thr Asp Thr Ala Glu
545 550
<210> 10
<211> 1231
<212> БЕЛОК
<213> Bacillus sp.
<400> 10
Leu Gln His Pro Gln Thr Tyr Val Asn Ile Gly Leu Ser Leu Ile Thr
1 5 10 15
Ile Ala Val Pro Glu Ile Gly Ile Phe Thr Pro Phe Ile Gly Leu Phe
20 25 30
Phe Ala Ala Leu Asn Lys His Asp Ala Pro Pro Pro Pro Asn Ala Lys
35 40 45
Asp Ile Phe Glu Ala Met Lys Pro Ala Ile Gln Glu Met Ile Asp Arg
50 55 60
Thr Leu Ser Ala Asp Glu Gln Thr Phe Leu Asn Gly Glu Val Ser Gly
65 70 75 80
Leu Gln Asn Leu Ala Ala Arg Tyr Gln Ser Thr Met Asp Asp Ile Gln
85 90 95
Ser His Gly Gly Phe Asn Lys Val Asp Ser Gly Leu Ile Lys Lys Phe
100 105 110
Thr Asp Glu Val Leu Ser Leu Asn Ser Phe Tyr Thr Asp Arg Leu Pro
115 120 125
Val Phe Ile Thr Asp Asn Thr Ala Asp Arg Thr Leu Leu Gly Leu Pro
130 135 140
Tyr Tyr Ala Ile Leu Ala Ser Met His Leu Met Leu Leu Arg Asp Ile
145 150 155 160
Ile Thr Lys Gly Pro Thr Trp Asp Ser Lys Ile Asn Phe Thr Pro Asp
165 170 175
Ala Ile Asp Ser Phe Lys Thr Asp Ile Lys Asn Asn Ile Lys Leu Tyr
180 185 190
Ser Lys Thr Ile Tyr Asp Val Phe Gln Lys Gly Leu Ala Ser Tyr Gly
195 200 205
Thr Pro Ser Asp Leu Glu Ser Phe Ala Lys Lys Gln Lys Tyr Ile Glu
210 215 220
Ile Met Thr Thr His Cys Leu Asp Phe Ala Arg Leu Phe Pro Thr Phe
225 230 235 240
Asp Pro Asp Leu Tyr Pro Thr Gly Ser Gly Asp Ile Ser Leu Gln Lys
245 250 255
Thr Arg Arg Ile Leu Ser Pro Leu Ile Pro Ile Arg Ala Val Asn Pro
260 265 270
Leu Ser Leu Glu Gly Thr Pro Val Asp Thr Ser Asn Trp Pro Asp Tyr
275 280 285
Glu Asn Gly Asn Gly Leu Leu Pro Asn Pro Arg Asp Arg Val Leu Lys
290 295 300
Gln Leu Thr Phe His Ala Ser Ser Arg Gly Gly Pro Gln Pro Gly Ala
305 310 315 320
Ser Val Leu His Pro Tyr Leu Trp Gly Val Glu Val Leu Asp Ser Val
325 330 335
Glu Ser Arg Leu Tyr Gly Lys Leu Pro Thr Val Asp Pro Gln Lys Ala
340 345 350
Pro Asn Asn Val Tyr Ile Tyr Pro Ser Asn Pro Ile Val Gln Ile Asn
355 360 365
Met Asp Thr Trp Lys Tyr Gln Pro Gln Ala Asp Arg Trp Asp Thr Asn
370 375 380
Leu Gly His Gly Ser Val Asp Arg Leu Gly Phe Leu Gln Gln Asp Gly
385 390 395 400
Thr Trp Ile Asn Leu Asp Arg Gly Pro Gly Asp Thr Thr Tyr Ser Leu
405 410 415
Pro Ala Ala His Tyr Leu Ser Tyr Leu Tyr Gly Thr Pro Phe Asn Thr
420 425 430
Asp Asn Ala Tyr Asp Ala His Val Ser Ala Leu Ala Gly Val Ser Thr
435 440 445
Pro Gln Glu Ala Ile Gly Asp Arg Phe Ile Gly Thr Pro Asp Glu Asp
450 455 460
Gly Asn Val Ser Ser Tyr Gly Phe Ala Trp Glu Asn Gly Val Ile Asp
465 470 475 480
Asn Gly Ile Gly Ser Leu Gln Val Val Lys Glu Thr Ile Thr Gly Thr
485 490 495
Asn Val Val Lys Leu Asn Tyr Gln Gln Ile Leu Lys Met Ser Met Gly
500 505 510
Asn Ser Val Arg Glu Ile Pro Tyr Ser Ile Arg Val His Tyr Ala Ser
515 520 525
Lys Asn Asp Val Thr Gly Tyr Phe His Met Asn Thr Leu Asn Gly Asp
530 535 540
Gln Leu Val Gly Glu Val Thr Phe Pro Ser Thr Glu Val Asp Asp Val
545 550 555 560
Lys Lys Met Val Ser Val Gln Gly Asp Asn Gly Lys Tyr Ala Leu Lys
565 570 575
Glu Ile Ala Lys Asn Phe Ile Ile Pro Arg Gly Thr Phe Asp Val Tyr
580 585 590
Ile Gln Asn Asn Ser Glu Glu Asp Phe Tyr Leu Asp Arg Ile Glu Phe
595 600 605
Val Pro Tyr Val Ala Pro Val Glu Pro Asp Pro Thr Tyr Thr Lys Ile
610 615 620
Ala Asp Ile Gln Pro Arg Asp Ile Pro Asn Asp Asp Gly Ile Thr Ile
625 630 635 640
Trp Glu Ser Ser Asp Gly Lys Ser Ala Asp Lys Val Asp Val Thr Val
645 650 655
Lys Ile Asn Gln Asn Val Phe Tyr Leu Glu Gly Ala Val Tyr Tyr Gln
660 665 670
Gly Asn Ala Ile Gly Asn Ile Met Asp Leu Asn Ser Pro Ser Pro Gly
675 680 685
Glu Gln Ser Phe Thr Trp Asn Lys Gly Arg Phe Asp Lys Val Thr Leu
690 695 700
Tyr Gly Leu Ile Ile Glu Asp Glu Pro Thr Phe Ala Gly Ser Val Val
705 710 715 720
Ser Gly Ala Ile Tyr Arg Ala Asn Glu Asn Thr Arg Asn Tyr Phe Thr
725 730 735
Lys Pro Arg Asp Leu Thr Asn Ile Thr Asn Gln Val His Ser Leu Phe
740 745 750
Val Ser Gly Thr Gln Asn Arg Leu Ala Gln Asn Ile Ser Asp His Asp
755 760 765
Ile Glu Glu Val Val Leu Lys Val Asp Ala Leu Ser Asp Glu Val Phe
770 775 780
Gly Glu Glu Lys Lys Ala Leu Arg Lys Leu Val Asn Gln Ala Lys Arg
785 790 795 800
Leu Ser Lys Ala Arg Asn Leu Leu Val Gly Gly Ser Phe Glu Asn Trp
805 810 815
Asp Ala Trp Tyr Lys Gly Arg Ser Val Val Arg Leu Ser Asp His Glu
820 825 830
Leu Leu Lys Ser Asp Tyr Val Phe Leu Pro Pro Pro Arg Phe Ser Pro
835 840 845
Ser Tyr Ile Phe Gln Lys Val Glu Glu Ser Lys Leu Lys Ala Asn Thr
850 855 860
Arg Tyr Val Val Ser Gly Phe Ile Ala His Ala Glu Asp Leu Glu Ile
865 870 875 880
Val Val Ser Arg Tyr Gly Gln Glu Ile Gln Lys Val Val Gln Val Pro
885 890 895
Tyr Gly Glu Ala Phe Pro Leu Thr Ser Gly Gly Val Ile Cys Cys Met
900 905 910
Ser Arg Pro Met Ser Asp Arg Lys Ser Ala Asp Pro His Phe Phe Ser
915 920 925
Tyr Ser Ile Asp Val Gly Glu Leu Asp Met Thr Ala Gly Pro Gly Ile
930 935 940
Glu Phe Gly Leu Arg Ile Val Gly Arg Thr Gly Met Ala Ser Val Ser
945 950 955 960
Asn Leu Glu Ile Arg Glu Glu Arg Ser Leu Lys Ala Ser Glu Ile Arg
965 970 975
Gln Val Gln Arg Val Ala Arg Asn Trp Arg Thr Glu Tyr Glu Lys Glu
980 985 990
Arg Ala Glu Val Thr Ser Leu Ile Gln Pro Val Ile Asn Arg Ile Asn
995 1000 1005
Gly Leu Tyr Glu Asn Gly Asn Trp Asn Gly Ser Ile Arg Ser Asp
1010 1015 1020
Ile Ser Tyr Gln Asn Ile Asp Ala Ile Val Leu Leu Thr Leu Pro
1025 1030 1035
Lys Leu Arg His Trp Phe Met Ser Asp Arg Phe Ser Glu Gln Gly
1040 1045 1050
Asp Ile Met Ala Lys Phe Gln Ser Ala Leu Asn Arg Ala Tyr Ala
1055 1060 1065
Gln Leu Glu Gln Asn Thr Leu Leu His Asn Gly His Phe Thr Lys
1070 1075 1080
Asp Ala Ala Asn Trp Thr Ile Glu Gly Asp Ala His Gln Ile Thr
1085 1090 1095
Leu Glu Asp Gly Arg His Val Leu Arg Leu Pro Asp Trp Ser Ser
1100 1105 1110
Ser Val Ser Gln Met Ile Glu Ile Glu Asn Phe Asp Pro Asp Lys
1115 1120 1125
Glu Tyr Asn Leu Val Leu His Gly Gln Gly Glu Gly Thr Val Thr
1130 1135 1140
Leu Glu His Gly Glu Glu Thr Lys Tyr Ile Glu Thr His Thr His
1145 1150 1155
His Phe Ala Asn Phe Thr Thr Ser Gln Arg Gln Gly Leu Thr Phe
1160 1165 1170
Glu Ser Asn Lys Val Thr Val Thr Ile Ser Ser Glu Asp Gly Glu
1175 1180 1185
Phe Leu Val Asp Asn Ile Ala Leu Val Glu Val Pro Met Phe Asn
1190 1195 1200
Lys Asn Gln Met Val Asn Glu Asn Lys Gly Val Asn Ile Asn Ser
1205 1210 1215
Asn Thr Asn Met Asn Ser Ser Asn Asn Ser Asn Asn Gln
1220 1225 1230
<210> 11
<211> 611
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 11
Met Gln His Pro Gln Thr Tyr Val Asn Ile Gly Leu Ser Leu Ile Thr
1 5 10 15
Ile Ala Val Pro Glu Ile Gly Ile Phe Thr Pro Phe Ile Gly Leu Phe
20 25 30
Phe Ala Ala Leu Asn Lys His Asp Ala Pro Pro Pro Pro Asn Ala Lys
35 40 45
Asp Ile Phe Glu Ala Met Lys Pro Ala Ile Gln Glu Met Ile Asp Arg
50 55 60
Thr Leu Ser Ala Asp Glu Gln Thr Phe Leu Asn Gly Glu Val Ser Gly
65 70 75 80
Leu Gln Asn Leu Ala Ala Arg Tyr Gln Ser Thr Met Asp Asp Ile Gln
85 90 95
Ser His Gly Gly Phe Asn Lys Val Asp Ser Gly Leu Ile Lys Lys Phe
100 105 110
Thr Asp Glu Val Leu Ser Leu Asn Ser Phe Tyr Thr Asp Arg Leu Pro
115 120 125
Val Phe Ile Thr Asp Asn Thr Ala Asp Arg Thr Leu Leu Gly Leu Pro
130 135 140
Tyr Tyr Ala Ile Leu Ala Ser Met His Leu Met Leu Leu Arg Asp Ile
145 150 155 160
Ile Thr Lys Gly Pro Thr Trp Asp Ser Lys Ile Asn Phe Thr Pro Asp
165 170 175
Ala Ile Asp Ser Phe Lys Thr Asp Ile Lys Asn Asn Ile Lys Leu Tyr
180 185 190
Ser Lys Thr Ile Tyr Asp Val Phe Gln Lys Gly Leu Ala Ser Tyr Gly
195 200 205
Thr Pro Ser Asp Leu Glu Ser Phe Ala Lys Lys Gln Lys Tyr Ile Glu
210 215 220
Ile Met Thr Thr His Cys Leu Asp Phe Ala Arg Leu Phe Pro Thr Phe
225 230 235 240
Asp Pro Asp Leu Tyr Pro Thr Gly Ser Gly Asp Ile Ser Leu Gln Lys
245 250 255
Thr Arg Arg Ile Leu Ser Pro Leu Ile Pro Ile Arg Ala Val Asn Pro
260 265 270
Leu Ser Leu Glu Gly Thr Pro Val Asp Thr Ser Asn Trp Pro Asp Tyr
275 280 285
Glu Asn Gly Asn Gly Leu Leu Pro Asn Pro Arg Asp Arg Val Leu Lys
290 295 300
Gln Leu Thr Phe His Ala Ser Ser Arg Gly Gly Pro Gln Pro Gly Ala
305 310 315 320
Ser Val Leu His Pro Tyr Leu Trp Gly Val Glu Val Leu Asp Ser Val
325 330 335
Glu Ser Arg Leu Tyr Gly Lys Leu Pro Thr Val Asp Pro Gln Lys Ala
340 345 350
Pro Asn Asn Val Tyr Ile Tyr Pro Ser Asn Pro Ile Val Gln Ile Asn
355 360 365
Met Asp Thr Trp Lys Tyr Gln Pro Gln Ala Asp Arg Trp Asp Thr Asn
370 375 380
Leu Gly His Gly Ser Val Asp Arg Leu Gly Phe Leu Gln Gln Asp Gly
385 390 395 400
Thr Trp Ile Asn Leu Asp Arg Gly Pro Gly Asp Thr Thr Tyr Ser Leu
405 410 415
Pro Ala Ala His Tyr Leu Ser Tyr Leu Tyr Gly Thr Pro Phe Asn Thr
420 425 430
Asp Asn Ala Tyr Asp Ala His Val Ser Ala Leu Ala Gly Val Ser Thr
435 440 445
Pro Gln Glu Ala Ile Gly Asp Arg Phe Ile Gly Thr Pro Asp Glu Asp
450 455 460
Gly Asn Val Ser Ser Tyr Gly Phe Ala Trp Glu Asn Gly Val Ile Asp
465 470 475 480
Asn Gly Ile Gly Ser Leu Gln Val Val Lys Glu Thr Ile Thr Gly Thr
485 490 495
Asn Val Val Lys Leu Asn Tyr Gln Gln Ile Leu Lys Met Ser Met Gly
500 505 510
Asn Ser Val Arg Glu Ile Pro Tyr Ser Ile Arg Val His Tyr Ala Ser
515 520 525
Lys Asn Asp Val Thr Gly Tyr Phe His Met Asn Thr Leu Asn Gly Asp
530 535 540
Gln Leu Val Gly Glu Val Thr Phe Pro Ser Thr Glu Val Asp Asp Val
545 550 555 560
Lys Lys Met Val Ser Val Gln Gly Asp Asn Gly Lys Tyr Ala Leu Lys
565 570 575
Glu Ile Ala Lys Asn Phe Ile Ile Pro Arg Gly Thr Phe Asp Val Tyr
580 585 590
Ile Gln Asn Asn Ser Glu Glu Asp Phe Tyr Leu Asp Arg Ile Glu Phe
595 600 605
Val Pro Tyr
610
<210> 12
<211> 1231
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 12
Met Gln His Pro Gln Thr Tyr Val Asn Ile Gly Leu Ser Leu Ile Thr
1 5 10 15
Ile Ala Val Pro Glu Ile Gly Ile Phe Thr Pro Phe Ile Gly Leu Phe
20 25 30
Phe Ala Ala Leu Asn Lys His Asp Ala Pro Pro Pro Pro Asn Ala Lys
35 40 45
Asp Ile Phe Glu Ala Met Lys Pro Ala Ile Gln Glu Met Ile Asp Arg
50 55 60
Thr Leu Ser Ala Asp Glu Gln Thr Phe Leu Asn Gly Glu Val Ser Gly
65 70 75 80
Leu Gln Asn Leu Ala Ala Arg Tyr Gln Ser Thr Met Asp Asp Ile Gln
85 90 95
Ser His Gly Gly Phe Asn Lys Val Asp Ser Gly Leu Ile Lys Lys Phe
100 105 110
Thr Asp Glu Val Leu Ser Leu Asn Ser Phe Tyr Thr Asp Arg Leu Pro
115 120 125
Val Phe Ile Thr Asp Asn Thr Ala Asp Arg Thr Leu Leu Gly Leu Pro
130 135 140
Tyr Tyr Ala Ile Leu Ala Ser Met His Leu Met Leu Leu Arg Asp Ile
145 150 155 160
Ile Thr Lys Gly Pro Thr Trp Asp Ser Lys Ile Asn Phe Thr Pro Asp
165 170 175
Ala Ile Asp Ser Phe Lys Thr Asp Ile Lys Asn Asn Ile Lys Leu Tyr
180 185 190
Ser Lys Thr Ile Tyr Asp Val Phe Gln Lys Gly Leu Ala Ser Tyr Gly
195 200 205
Thr Pro Ser Asp Leu Glu Ser Phe Ala Lys Lys Gln Lys Tyr Ile Glu
210 215 220
Ile Met Thr Thr His Cys Leu Asp Phe Ala Arg Leu Phe Pro Thr Phe
225 230 235 240
Asp Pro Asp Leu Tyr Pro Thr Gly Ser Gly Asp Ile Ser Leu Gln Lys
245 250 255
Thr Arg Arg Ile Leu Ser Pro Leu Ile Pro Ile Arg Ala Val Asn Pro
260 265 270
Leu Ser Leu Glu Gly Thr Pro Val Asp Thr Ser Asn Trp Pro Asp Tyr
275 280 285
Glu Asn Gly Asn Gly Leu Leu Pro Asn Pro Arg Asp Arg Val Leu Lys
290 295 300
Gln Leu Thr Phe His Ala Ser Ser Arg Gly Gly Pro Gln Pro Gly Ala
305 310 315 320
Ser Val Leu His Pro Tyr Leu Trp Gly Val Glu Val Leu Asp Ser Val
325 330 335
Glu Ser Arg Leu Tyr Gly Lys Leu Pro Thr Val Asp Pro Gln Lys Ala
340 345 350
Pro Asn Asn Val Tyr Ile Tyr Pro Ser Asn Pro Ile Val Gln Ile Asn
355 360 365
Met Asp Thr Trp Lys Tyr Gln Pro Gln Ala Asp Arg Trp Asp Thr Asn
370 375 380
Leu Gly His Gly Ser Val Asp Arg Leu Gly Phe Leu Gln Gln Asp Gly
385 390 395 400
Thr Trp Ile Asn Leu Asp Arg Gly Pro Gly Asp Thr Thr Tyr Ser Leu
405 410 415
Pro Ala Ala His Tyr Leu Ser Tyr Leu Tyr Gly Thr Pro Phe Asn Thr
420 425 430
Asp Asn Ala Tyr Asp Ala His Val Ser Ala Leu Ala Gly Val Ser Thr
435 440 445
Pro Gln Glu Ala Ile Gly Asp Arg Phe Ile Gly Thr Pro Asp Glu Asp
450 455 460
Gly Asn Val Ser Ser Tyr Gly Phe Ala Trp Glu Asn Gly Val Ile Asp
465 470 475 480
Asn Gly Ile Gly Ser Leu Gln Val Val Lys Glu Thr Ile Thr Gly Thr
485 490 495
Asn Val Val Lys Leu Asn Tyr Gln Gln Ile Leu Lys Met Ser Met Gly
500 505 510
Asn Ser Val Arg Glu Ile Pro Tyr Ser Ile Arg Val His Tyr Ala Ser
515 520 525
Lys Asn Asp Val Thr Gly Tyr Phe His Met Asn Thr Leu Asn Gly Asp
530 535 540
Gln Leu Val Gly Glu Val Thr Phe Pro Ser Thr Glu Val Asp Asp Val
545 550 555 560
Lys Lys Met Val Ser Val Gln Gly Asp Asn Gly Lys Tyr Ala Leu Lys
565 570 575
Glu Ile Ala Lys Asn Phe Ile Ile Pro Arg Gly Thr Phe Asp Val Tyr
580 585 590
Ile Gln Asn Asn Ser Glu Glu Asp Phe Tyr Leu Asp Arg Ile Glu Phe
595 600 605
Val Pro Tyr Val Ala Pro Val Glu Pro Asp Pro Thr Tyr Thr Lys Ile
610 615 620
Ala Asp Ile Gln Pro Arg Asp Ile Pro Asn Asp Asp Gly Ile Thr Ile
625 630 635 640
Trp Glu Ser Ser Asp Gly Lys Ser Ala Asp Lys Val Asp Val Thr Val
645 650 655
Lys Ile Asn Gln Asn Val Phe Tyr Leu Glu Gly Ala Val Tyr Tyr Gln
660 665 670
Gly Asn Ala Ile Gly Asn Ile Met Asp Leu Asn Ser Pro Ser Pro Gly
675 680 685
Glu Gln Ser Phe Thr Trp Asn Lys Gly Arg Phe Asp Lys Val Thr Leu
690 695 700
Tyr Gly Leu Ile Ile Glu Asp Glu Pro Thr Phe Ala Gly Ser Val Val
705 710 715 720
Ser Gly Ala Ile Tyr Arg Ala Asn Glu Asn Thr Arg Asn Tyr Phe Thr
725 730 735
Lys Pro Arg Asp Leu Thr Asn Ile Thr Asn Gln Val His Ser Leu Phe
740 745 750
Val Ser Gly Thr Gln Asn Arg Leu Ala Gln Asn Ile Ser Asp His Asp
755 760 765
Ile Glu Glu Val Val Leu Lys Val Asp Ala Leu Ser Asp Glu Val Phe
770 775 780
Gly Glu Glu Lys Lys Ala Leu Arg Lys Leu Val Asn Gln Ala Lys Arg
785 790 795 800
Leu Ser Lys Ala Arg Asn Leu Leu Val Gly Gly Ser Phe Glu Asn Trp
805 810 815
Asp Ala Trp Tyr Lys Gly Arg Ser Val Val Arg Leu Ser Asp His Glu
820 825 830
Leu Leu Lys Ser Asp Tyr Val Phe Leu Pro Pro Pro Arg Phe Ser Pro
835 840 845
Ser Tyr Ile Phe Gln Lys Val Glu Glu Ser Lys Leu Lys Ala Asn Thr
850 855 860
Arg Tyr Val Val Ser Gly Phe Ile Ala His Ala Glu Asp Leu Glu Ile
865 870 875 880
Val Val Ser Arg Tyr Gly Gln Glu Ile Gln Lys Val Val Gln Val Pro
885 890 895
Tyr Gly Glu Ala Phe Pro Leu Thr Ser Gly Gly Val Ile Cys Cys Met
900 905 910
Ser Arg Pro Met Ser Asp Arg Lys Ser Ala Asp Pro His Phe Phe Ser
915 920 925
Tyr Ser Ile Asp Val Gly Glu Leu Asp Met Thr Ala Gly Pro Gly Ile
930 935 940
Glu Phe Gly Leu Arg Ile Val Gly Arg Thr Gly Met Ala Ser Val Ser
945 950 955 960
Asn Leu Glu Ile Arg Glu Glu Arg Ser Leu Lys Ala Ser Glu Ile Arg
965 970 975
Gln Val Gln Arg Val Ala Arg Asn Trp Arg Thr Glu Tyr Glu Lys Glu
980 985 990
Arg Ala Glu Val Thr Ser Leu Ile Gln Pro Val Ile Asn Arg Ile Asn
995 1000 1005
Gly Leu Tyr Glu Asn Gly Asn Trp Asn Gly Ser Ile Arg Ser Asp
1010 1015 1020
Ile Ser Tyr Gln Asn Ile Asp Ala Ile Val Leu Leu Thr Leu Pro
1025 1030 1035
Lys Leu Arg His Trp Phe Met Ser Asp Arg Phe Ser Glu Gln Gly
1040 1045 1050
Asp Ile Met Ala Lys Phe Gln Ser Ala Leu Asn Arg Ala Tyr Ala
1055 1060 1065
Gln Leu Glu Gln Asn Thr Leu Leu His Asn Gly His Phe Thr Lys
1070 1075 1080
Asp Ala Ala Asn Trp Thr Ile Glu Gly Asp Ala His Gln Ile Thr
1085 1090 1095
Leu Glu Asp Gly Arg His Val Leu Arg Leu Pro Asp Trp Ser Ser
1100 1105 1110
Ser Val Ser Gln Met Ile Glu Ile Glu Asn Phe Asp Pro Asp Lys
1115 1120 1125
Glu Tyr Asn Leu Val Leu His Gly Gln Gly Glu Gly Thr Val Thr
1130 1135 1140
Leu Glu His Gly Glu Glu Thr Lys Tyr Ile Glu Thr His Thr His
1145 1150 1155
His Phe Ala Asn Phe Thr Thr Ser Gln Arg Gln Gly Leu Thr Phe
1160 1165 1170
Glu Ser Asn Lys Val Thr Val Thr Ile Ser Ser Glu Asp Gly Glu
1175 1180 1185
Phe Leu Val Asp Asn Ile Ala Leu Val Glu Val Pro Met Phe Asn
1190 1195 1200
Lys Asn Gln Met Val Asn Glu Asn Lys Gly Val Asn Ile Asn Ser
1205 1210 1215
Asn Thr Asn Met Asn Ser Ser Asn Asn Ser Asn Asn Gln
1220 1225 1230
<210> 13
<211> 611
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 13
Leu Gln His Pro Gln Thr Tyr Val Asn Ile Gly Leu Ser Leu Ile Thr
1 5 10 15
Ile Ala Val Pro Glu Ile Gly Ile Phe Thr Pro Phe Ile Gly Leu Phe
20 25 30
Phe Ala Ala Leu Asn Lys His Asp Ala Pro Pro Pro Pro Asn Ala Lys
35 40 45
Asp Ile Phe Glu Ala Met Lys Pro Ala Ile Gln Glu Met Ile Asp Arg
50 55 60
Thr Leu Ser Ala Asp Glu Gln Thr Phe Leu Asn Gly Glu Val Ser Gly
65 70 75 80
Leu Gln Asn Leu Ala Ala Arg Tyr Gln Ser Thr Met Asp Asp Ile Gln
85 90 95
Ser His Gly Gly Phe Asn Lys Val Asp Ser Gly Leu Ile Lys Lys Phe
100 105 110
Thr Asp Glu Val Leu Ser Leu Asn Ser Phe Tyr Thr Asp Arg Leu Pro
115 120 125
Val Phe Ile Thr Asp Asn Thr Ala Asp Arg Thr Leu Leu Gly Leu Pro
130 135 140
Tyr Tyr Ala Ile Leu Ala Ser Met His Leu Met Leu Leu Arg Asp Ile
145 150 155 160
Ile Thr Lys Gly Pro Thr Trp Asp Ser Lys Ile Asn Phe Thr Pro Asp
165 170 175
Ala Ile Asp Ser Phe Lys Thr Asp Ile Lys Asn Asn Ile Lys Leu Tyr
180 185 190
Ser Lys Thr Ile Tyr Asp Val Phe Gln Lys Gly Leu Ala Ser Tyr Gly
195 200 205
Thr Pro Ser Asp Leu Glu Ser Phe Ala Lys Lys Gln Lys Tyr Ile Glu
210 215 220
Ile Met Thr Thr His Cys Leu Asp Phe Ala Arg Leu Phe Pro Thr Phe
225 230 235 240
Asp Pro Asp Leu Tyr Pro Thr Gly Ser Gly Asp Ile Ser Leu Gln Lys
245 250 255
Thr Arg Arg Ile Leu Ser Pro Leu Ile Pro Ile Arg Ala Val Asn Pro
260 265 270
Leu Ser Leu Glu Gly Thr Pro Val Asp Thr Ser Asn Trp Pro Asp Tyr
275 280 285
Glu Asn Gly Asn Gly Leu Leu Pro Asn Pro Arg Asp Arg Val Leu Lys
290 295 300
Gln Leu Thr Phe His Ala Ser Ser Arg Gly Gly Pro Gln Pro Gly Ala
305 310 315 320
Ser Val Leu His Pro Tyr Leu Trp Gly Val Glu Val Leu Asp Ser Val
325 330 335
Glu Ser Arg Leu Tyr Gly Lys Leu Pro Thr Val Asp Pro Gln Lys Ala
340 345 350
Pro Asn Asn Val Tyr Ile Tyr Pro Ser Asn Pro Ile Val Gln Ile Asn
355 360 365
Met Asp Thr Trp Lys Tyr Gln Pro Gln Ala Asp Arg Trp Asp Thr Asn
370 375 380
Leu Gly His Gly Ser Val Asp Arg Leu Gly Phe Leu Gln Gln Asp Gly
385 390 395 400
Thr Trp Ile Asn Leu Asp Arg Gly Pro Gly Asp Thr Thr Tyr Ser Leu
405 410 415
Pro Ala Ala His Tyr Leu Ser Tyr Leu Tyr Gly Thr Pro Phe Asn Thr
420 425 430
Asp Asn Ala Tyr Asp Ala His Val Ser Ala Leu Ala Gly Val Ser Thr
435 440 445
Pro Gln Glu Ala Ile Gly Asp Arg Phe Ile Gly Thr Pro Asp Glu Asp
450 455 460
Gly Asn Val Ser Ser Tyr Gly Phe Ala Trp Glu Asn Gly Val Ile Asp
465 470 475 480
Asn Gly Ile Gly Ser Leu Gln Val Val Lys Glu Thr Ile Thr Gly Thr
485 490 495
Asn Val Val Lys Leu Asn Tyr Gln Gln Ile Leu Lys Met Ser Met Gly
500 505 510
Asn Ser Val Arg Glu Ile Pro Tyr Ser Ile Arg Val His Tyr Ala Ser
515 520 525
Lys Asn Asp Val Thr Gly Tyr Phe His Met Asn Thr Leu Asn Gly Asp
530 535 540
Gln Leu Val Gly Glu Val Thr Phe Pro Ser Thr Glu Val Asp Asp Val
545 550 555 560
Lys Lys Met Val Ser Val Gln Gly Asp Asn Gly Lys Tyr Ala Leu Lys
565 570 575
Glu Ile Ala Lys Asn Phe Ile Ile Pro Arg Gly Thr Phe Asp Val Tyr
580 585 590
Ile Gln Asn Asn Ser Glu Glu Asp Phe Tyr Leu Asp Arg Ile Glu Phe
595 600 605
Val Pro Tyr
610
<210> 14
<211> 608
<212> БЕЛОК
<213> Bacillus sp.
<400> 14
Met Ile Ile Gly Met Val Thr Lys Ala Val Thr Pro Lys Leu Gly Ala
1 5 10 15
Leu Pro Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Ser
20 25 30
Gly Ile Thr Asp Val Met Ser Thr Ile Phe Gly Val Asn Thr Gly Asp
35 40 45
Ser Ser Ile Asp Asn Val Met Asn Asn Glu Ala Leu Leu Lys Gln Met
50 55 60
Met Asp Gln Met Asn Ala Met Gln Ser Ser Leu Asp Asp Ile Leu Lys
65 70 75 80
Asn Gln Lys Met Ser Glu Val Leu Gln His Met Leu Val Asn Leu Ser
85 90 95
Asn Glu Gln Leu Asp Leu Ser Lys Ser Ile Asn Val Glu Leu Gly Lys
100 105 110
Ile Glu Gly Ile Leu Thr Thr Tyr Leu Pro Ala Ile Ser Ser Met Leu
115 120 125
Asn Asn Ile Tyr Ser Gln Thr Ser Glu Ile Asn Gln Lys Val Asp Lys
130 135 140
Leu Leu Ala Leu Met Thr Phe Ala Leu Gln Glu Leu Glu Tyr Ile Lys
145 150 155 160
Asp Asn Val Ile Leu Asn Ser Ser Ile Val Glu Ile Thr Pro His Val
165 170 175
Gln Lys Leu Val Tyr Val Asn Lys Lys Phe Leu Glu Leu Ser Gly Met
180 185 190
Tyr Met Ser Gly Asp Ile Ser Ile Asp Asn Met Gln Glu Leu Ile Gln
195 200 205
Trp Ala Lys Ser Ile Val Asp Thr Asp Met Asn Ser Phe Glu Phe Ser
210 215 220
Ile Asp Ser Leu His Asn Thr Ile Val Gly Asp Asn Leu Tyr Lys Arg
225 230 235 240
Ser Ala Leu Lys Thr Phe Ala Asp Val Leu Met Asp Asp Asp Ser Lys
245 250 255
Tyr Gly Asp Phe Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser
260 265 270
Ser Leu Ala Thr Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Val Ala
275 280 285
Arg Lys Val Leu Gly Leu Thr Asp Ile Asn Tyr Ser Leu Thr Met His
290 295 300
Glu Arg Met Glu Gln Gln Asn Gln Leu Phe Leu Asn Ile Ile Gln Asp
305 310 315 320
Gln Lys Ile Ser Asn Tyr Leu Lys Ile Lys Gly Thr Tyr Ser Met Asp
325 330 335
Ser Gln Tyr Val Asp Glu Leu Lys Thr Ser Asp Leu Gln Ala Lys Asp
340 345 350
Gly Tyr Ala Leu Ile Gly Leu Glu Phe Phe Val Asp Asn Gly Ile Tyr
355 360 365
Lys Ala Lys Ala Tyr Gln Gly Lys Ile Asp Thr Asn Phe Ser Val His
370 375 380
Ala Asn Thr Leu Glu Glu Ile Val Ser Asp Asp Leu Ser Glu Val Phe
385 390 395 400
Pro Glu Gly Ile Arg Lys Asp Gly Thr Ser Val Arg Tyr Pro Leu Ser
405 410 415
Gly Asn Leu Ala Gly Pro Asp Asn Ser Val Ile Thr Arg Ile Gly Phe
420 425 430
Gly Ser Lys Tyr Val Ser Lys Asp Asp Cys Tyr Ala Tyr Ile Glu Ala
435 440 445
Asp Phe Ser Pro Tyr Asp Pro Ser Ser Gly Gln Val Met Lys Glu Gly
450 455 460
Thr Gln Thr Ile Ser Leu Gln Gly Asn Lys Gly Thr Thr Ile Ala Tyr
465 470 475 480
Asp Thr Trp Pro Ile Gly Leu Ile Ser Asp Phe Tyr Met Ser Pro Leu
485 490 495
Lys Asn Leu Ser Leu Tyr Val Asp Val Asn Glu Asn His Gly Tyr Ser
500 505 510
Val Met Asn Phe Gly Gly Glu Ser Tyr Tyr Tyr Thr Met Leu Ser Arg
515 520 525
Glu Tyr Asp Ala Asn Phe Ile Leu Val Ser Tyr Thr Glu Asn Gly Ser
530 535 540
Arg Lys Pro Glu Asn Leu Ile Glu Asn Gly Asp Phe Glu Asn Lys Gly
545 550 555 560
Asp Gln Trp Asp Phe Lys Thr Tyr Gly Gln His Asp Lys Pro Tyr Val
565 570 575
Ala Glu Gly Ile Gly Leu Asp Gly Ser Asn Ala Leu Ile Ile Glu Asp
580 585 590
Gly Ile Ala Leu Gln Tyr Leu Asn Leu Lys Pro Tyr Thr Asn Ile Asn
595 600 605
<210> 15
<211> 682
<212> БЕЛОК
<213> Bacillus sp.
<400> 15
Met Ser Lys Leu Leu Ile Gly Gly Ile Phe Met Ser Ser Asn Asn Gln
1 5 10 15
Asn Asn Leu Asp Ile Val Ser Thr Ser Glu Lys Lys Phe Val Ser Lys
20 25 30
Glu His Ser Lys Asn Leu Leu Thr Asn Asp Leu His Asn Met Asn Tyr
35 40 45
Lys Asp Phe Leu Arg Met Ser Asp Glu His Asn Ala Glu Phe Leu Ala
50 55 60
Asn Ala Glu Val Phe Val Asn Thr Gln Asp Ser Ile Lys Leu Gly Met
65 70 75 80
Asn Ile Val Gly Glu Ile Leu Ser Ser Leu Gly Val Pro Phe Ile Gly
85 90 95
Pro Ile Val Ser Phe Tyr Thr Thr Ile Ile Asp Leu Leu Trp Pro Ser
100 105 110
Ser Gly Gly Lys Asn Pro Trp Glu Ile Phe Met Glu Gln Val Glu Glu
115 120 125
Leu Met Asp Gln Lys Ile Ala Glu Tyr Ala Arg Lys Lys Ala Leu Ala
130 135 140
Glu Leu Glu Gly Leu Gly Ala Asn Phe Glu Leu Tyr Arg Ile Ala Leu
145 150 155 160
Glu Glu Trp Lys Glu Asn Pro Asn Ile Ser Arg Thr Thr Arg Asp Val
165 170 175
Arg Thr Arg Phe Glu Ile Leu Asp Ser Leu Phe Thr Gln Tyr Met Pro
180 185 190
Ser Phe Lys Val Ser Gly Tyr Glu Val Pro Leu Leu Thr Val Tyr Thr
195 200 205
Gln Ala Ala Asn Leu His Leu Leu Leu Leu Arg Asp Ala Ser Ile Phe
210 215 220
Gly Glu Glu Trp Gly Leu Ser Thr Thr Thr Ile Asn Asn Tyr Tyr Lys
225 230 235 240
Arg Gln Ile Asn Leu Thr Ala Gln Tyr Ser Asp His Cys Val Lys Trp
245 250 255
Tyr Asn Thr Gly Leu Asp Arg Leu Lys Gly Ser Asn Ala Glu Ser Trp
260 265 270
Leu Lys Tyr His His Phe Arg Arg Glu Met Thr Leu Met Val Leu Asp
275 280 285
Ile Val Ala Leu Tyr Pro Asn Tyr Asp Thr His Thr Tyr Pro Ile Glu
290 295 300
Thr Lys Ala Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Leu Gly Tyr
305 310 315 320
Asn Pro Thr Asn Pro Glu Asp Asn Gly Phe Gly Ile Pro Trp Thr Arg
325 330 335
Leu Ser Phe His Asn Phe Ala Gln Ile Glu Lys Asn Ala Ile Arg Pro
340 345 350
Pro His Leu Phe Asp Ile Leu Lys Ser Val Glu Ile Ser Thr Ile Lys
355 360 365
Gly Thr Leu Ala Leu Asn Asn Lys Ala Tyr Ile Asn Phe Trp Ser Gly
370 375 380
His Ser Leu Lys Tyr Thr Tyr Thr Asn Asn Leu Glu Thr Tyr Glu Ser
385 390 395 400
Lys Tyr Gly Asn Pro Asn Thr Ser Glu Lys Asn Met Phe Asp Leu Met
405 410 415
Asp Arg Asp Ile Tyr Lys Ile Asp Ser Thr Ala Ala Cys Leu Ala Asn
420 425 430
Phe Tyr Thr Tyr Gln Tyr Gly Gly Val Lys Ala Asp Phe Leu Met Val
435 440 445
Lys Arg Asp Ser Ser Ser Thr Thr Ala Tyr Ser Tyr Ser Lys Thr His
450 455 460
Thr Ala Ile Gln Ser Arg Ile Gln Asn Tyr Asp Ser Glu Asn Glu Leu
465 470 475 480
Pro Gln Glu Thr Thr Asp Gln Pro Asn Tyr Gln Thr Tyr Asn His Arg
485 490 495
Leu Ser His Ile Thr Ser Tyr Ser Phe Ser Asn Ile Gly Asn Ser Asn
500 505 510
Gly Gln Phe Gly Asn Leu Pro Val Phe Val Trp Thr His Arg Ser Ala
515 520 525
Asn Ala Asp Asn Phe Ile Phe Leu Asp Lys Ile Thr Gln Leu Pro Leu
530 535 540
Thr Lys Ser Tyr Glu Ile Ser Asp Asn Ala Thr Ile Val Asn Ser Asp
545 550 555 560
Tyr Thr Gly Gly Ser Leu Leu Lys Val Asn Arg Ser Met Leu Lys Asn
565 570 575
Asn Thr Val Asn Ser Ile Arg Phe Ser Leu Pro Glu Ser Lys Arg Tyr
580 585 590
Ile Ala Arg Val Arg Tyr Ala Ser Thr Gly Asp Gly Tyr Met Asn Met
595 600 605
Thr Gly Ile Ser Ser Asp Thr Tyr Phe Asn Ala Thr Ile Asn Glu Glu
610 615 620
Ser Asp Leu Lys Tyr Asp Ser Phe Arg Tyr Leu Thr Leu Gly Thr Ile
625 630 635 640
Ser Ala Asp Pro Tyr Asn Phe Tyr Ser Leu Tyr Leu Pro Thr Pro Tyr
645 650 655
Gly Glu Lys Tyr Thr Ile Tyr Ile Asp Lys Ile Glu Leu Ile Pro Leu
660 665 670
Asp Glu Thr Tyr Glu Val Glu Lys Asn Phe
675 680
<210> 16
<211> 672
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 16
Met Ser Lys Leu Leu Ile Gly Gly Ile Phe Met Ser Ser Asn Asn Gln
1 5 10 15
Asn Asn Leu Asp Ile Val Ser Thr Ser Glu Lys Lys Phe Val Ser Lys
20 25 30
Glu His Ser Lys Asn Leu Leu Thr Asn Asp Leu His Asn Met Asn Tyr
35 40 45
Lys Asp Phe Leu Arg Met Ser Asp Glu His Asn Ala Glu Phe Leu Ala
50 55 60
Asn Ala Glu Val Phe Val Asn Thr Gln Asp Ser Ile Lys Leu Gly Met
65 70 75 80
Asn Ile Val Gly Glu Ile Leu Ser Ser Leu Gly Val Pro Phe Ile Gly
85 90 95
Pro Ile Val Ser Phe Tyr Thr Thr Ile Ile Asp Leu Leu Trp Pro Ser
100 105 110
Ser Gly Gly Lys Asn Pro Trp Glu Ile Phe Met Glu Gln Val Glu Glu
115 120 125
Leu Met Asp Gln Lys Ile Ala Glu Tyr Ala Arg Lys Lys Ala Leu Ala
130 135 140
Glu Leu Glu Gly Leu Gly Ala Asn Phe Glu Leu Tyr Arg Ile Ala Leu
145 150 155 160
Glu Glu Trp Lys Glu Asn Pro Asn Ile Ser Arg Thr Thr Arg Asp Val
165 170 175
Arg Thr Arg Phe Glu Ile Leu Asp Ser Leu Phe Thr Gln Tyr Met Pro
180 185 190
Ser Phe Lys Val Ser Gly Tyr Glu Val Pro Leu Leu Thr Val Tyr Thr
195 200 205
Gln Ala Ala Asn Leu His Leu Leu Leu Leu Arg Asp Ala Ser Ile Phe
210 215 220
Gly Glu Glu Trp Gly Leu Ser Thr Thr Thr Ile Asn Asn Tyr Tyr Lys
225 230 235 240
Arg Gln Ile Asn Leu Thr Ala Gln Tyr Ser Asp His Cys Val Lys Trp
245 250 255
Tyr Asn Thr Gly Leu Asp Arg Leu Lys Gly Ser Asn Ala Glu Ser Trp
260 265 270
Leu Lys Tyr His His Phe Arg Arg Glu Met Thr Leu Met Val Leu Asp
275 280 285
Ile Val Ala Leu Tyr Pro Asn Tyr Asp Thr His Thr Tyr Pro Ile Glu
290 295 300
Thr Lys Ala Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Leu Gly Tyr
305 310 315 320
Asn Pro Thr Asn Pro Glu Asp Asn Gly Phe Gly Ile Pro Trp Thr Arg
325 330 335
Leu Ser Phe His Asn Phe Ala Gln Ile Glu Lys Asn Ala Ile Arg Pro
340 345 350
Pro His Leu Phe Asp Ile Leu Lys Ser Val Glu Ile Ser Thr Ile Lys
355 360 365
Gly Thr Leu Ala Leu Asn Asn Lys Ala Tyr Ile Asn Phe Trp Ser Gly
370 375 380
His Ser Leu Lys Tyr Thr Tyr Thr Asn Asn Leu Glu Thr Tyr Glu Ser
385 390 395 400
Lys Tyr Gly Asn Pro Asn Thr Ser Glu Lys Asn Met Phe Asp Leu Met
405 410 415
Asp Arg Asp Ile Tyr Lys Ile Asp Ser Thr Ala Ala Cys Leu Ala Asn
420 425 430
Phe Tyr Thr Tyr Gln Tyr Gly Gly Val Lys Ala Asp Phe Leu Met Val
435 440 445
Lys Arg Asp Ser Ser Ser Thr Thr Ala Tyr Ser Tyr Ser Lys Thr His
450 455 460
Thr Ala Ile Gln Ser Arg Ile Gln Asn Tyr Asp Ser Glu Asn Glu Leu
465 470 475 480
Pro Gln Glu Thr Thr Asp Gln Pro Asn Tyr Gln Thr Tyr Asn His Arg
485 490 495
Leu Ser His Ile Thr Ser Tyr Ser Phe Ser Asn Ile Gly Asn Ser Asn
500 505 510
Gly Gln Phe Gly Asn Leu Pro Val Phe Val Trp Thr His Arg Ser Ala
515 520 525
Asn Ala Asp Asn Phe Ile Phe Leu Asp Lys Ile Thr Gln Leu Pro Leu
530 535 540
Thr Lys Ser Tyr Glu Ile Ser Asp Asn Ala Thr Ile Val Asn Ser Asp
545 550 555 560
Tyr Thr Gly Gly Ser Leu Leu Lys Val Asn Arg Ser Met Leu Lys Asn
565 570 575
Asn Thr Val Asn Ser Ile Arg Phe Ser Leu Pro Glu Ser Lys Arg Tyr
580 585 590
Ile Ala Arg Val Arg Tyr Ala Ser Thr Gly Asp Gly Tyr Met Asn Met
595 600 605
Thr Gly Ile Ser Ser Asp Thr Tyr Phe Asn Ala Thr Ile Asn Glu Glu
610 615 620
Ser Asp Leu Lys Tyr Asp Ser Phe Arg Tyr Leu Thr Leu Gly Thr Ile
625 630 635 640
Ser Ala Asp Pro Tyr Asn Phe Tyr Ser Leu Tyr Leu Pro Thr Pro Tyr
645 650 655
Gly Glu Lys Tyr Thr Ile Tyr Ile Asp Lys Ile Glu Leu Ile Pro Leu
660 665 670
<210> 17
<211> 1306
<212> БЕЛОК
<213> Bacillus sp.
<400> 17
Leu Arg Ile Lys Tyr Ala Tyr Asn Leu His His Leu Ile Ile Asn Asn
1 5 10 15
His Tyr Asn Glu Glu Lys Ser Pro Leu Gln Asn Gly Gly Ser Tyr Met
20 25 30
Asn Gln Asn Tyr Asn Asn Lys Glu Tyr Glu Ile Leu Ser Thr Gly Gly
35 40 45
Lys Asp Tyr Gln Pro Arg Tyr Pro Leu Ala Gln Glu Pro Asn Ala Glu
50 55 60
Leu Gln Gln Met Ser Tyr Lys Asp Trp Met Asn Arg Cys Ala Gly Val
65 70 75 80
Thr Ser Gly Glu Leu Phe Asp Ala Asp Ala Val Lys Thr Ala Leu Ile
85 90 95
Ile Gly Thr Gly Ile Ser Trp Ala Ile Leu Gly Val Ala Phe Pro Pro
100 105 110
Leu Ser Val Ala Val Gly Val Leu Asn Ile Ile Ile Ala Tyr Leu Trp
115 120 125
Pro Ser Gln Ala Thr Glu Asp Gln Phe Ser Trp Lys Gln Met Met Ser
130 135 140
Ala Thr Glu Gln Leu Ile Asn Gln Ala Ile Asn Pro Ile Ile Lys Ser
145 150 155 160
Lys Ala Ile Asp Ala Leu Arg Asp Leu Gln Ser Ser Leu Arg Asp Tyr
165 170 175
Asn Gln Ala Leu Cys Asn Leu Gln Thr Asn Leu Asp Ser Pro Asp Val
180 185 190
Glu Arg Tyr Lys Glu Val Val Arg Arg Glu Phe Asn Asp Ala Asn Asp
195 200 205
Lys Ala Lys Ser Thr Ile Ile Asn Leu Glu Ser Asp Gly Phe Gln Ile
210 215 220
Pro Phe Leu Ser Ser Tyr Ala Gln Ala Ala Asn Ile His Leu Leu Leu
225 230 235 240
Leu Lys Asp Val Lys Gln Tyr Gly Glu Ile Trp Gly Leu Thr Ala Leu
245 250 255
Glu Val Glu Gln Tyr Phe Asp Asn Pro Pro Ser Lys Pro Gly Asn Pro
260 265 270
Gly Met Ile Gln Leu Ile Gly Ile Tyr Thr Asp Tyr Cys Ile His Trp
275 280 285
Tyr Asn Arg Gly Leu Gln Gln Gln Ala Asp Arg Tyr Tyr Trp Ser Ser
290 295 300
Phe Asn Asp Phe Arg Arg Thr Met Thr Ile Met Val Leu Asp Ile Val
305 310 315 320
Ala Leu Trp Pro Thr Tyr Asn Pro Ile Asn Tyr Pro Lys Pro Val Lys
325 330 335
Thr Gln Leu Thr Arg Lys Val Tyr Thr Pro Ala Phe Gly Gln Pro Ile
340 345 350
Tyr Ser Ser Glu Trp Leu Phe Asn Glu Leu Glu Leu Ser Leu Val Thr
355 360 365
Pro Pro Arg Leu Phe Thr Phe Leu Asp Ser Val Thr Phe Tyr Arg Arg
370 375 380
Asp Asp Glu Leu Thr Gln Tyr Ala Gly Ile Met Gln Asn Leu Arg Tyr
385 390 395 400
Thr Ser Ser Leu Lys Leu Phe Tyr Thr Leu Gln Gly Gln Arg Thr Asp
405 410 415
Leu Val Asp Ser Ile Arg Ile Tyr Ser Gly Val Phe Asp Glu Val Trp
420 425 430
Lys Thr Ala Ser Val Val Gly Glu Phe Glu Asn Ala Gly Gly Thr Gln
435 440 445
Leu Gln Thr Phe Ser Phe Tyr Leu Leu Ser Asn Pro Glu Asn Pro Val
450 455 460
Thr Ile Gly His Val Tyr Ser Ser Asn Thr Thr Ala Ala Thr Tyr Gly
465 470 475 480
Phe Pro Cys Val Ala Thr Asn Ile Ser Cys Ile Gly Glu Pro Cys Thr
485 490 495
Pro Cys Ser Ile Ser Gln Pro Leu Glu Ser Ile Cys Asn Ile Pro Gly
500 505 510
Lys Ser Ser His Phe Leu Ala Ser Val Thr Ala Asp Val Tyr Gly His
515 520 525
Val Arg Ile Ala Ser Tyr Gly Trp Asn His Ile Ser Ala Asp Thr Val
530 535 540
Asn Leu Leu Asn Ala Glu Lys Ile Thr Gln Ile Pro Ala Val Lys Gly
545 550 555 560
Asn Thr Met Ile Gly Asp Ser Ser Val Val Lys Gly Pro Gly Ser Thr
565 570 575
Gly Gly Asp Leu Val Lys Met Ser Glu Arg Ala Val Phe Ser Leu Gly
580 585 590
Leu Thr Ile Pro Ala Lys Asn Glu Arg Leu Asn Gly Tyr Ala Ile Arg
595 600 605
Leu Arg Tyr Thr Ser Asn Ala Asp Ile Asn Phe Gln Ile Phe Ile Arg
610 615 620
Tyr Asn Ser Gln Lys Thr Val Val Ser Asn Gln Ile Ile Pro Pro Thr
625 630 635 640
Ser Phe Asp Asn Ser Leu Thr Tyr Gln Thr Phe Gly Tyr Tyr Thr Leu
645 650 655
Ser Glu Leu Glu Leu Glu Thr Leu Ser Glu Val Thr Pro Glu Ala Val
660 665 670
Ile Thr Ile Asp Lys Phe Gly Ala Gly Asp Ile Leu Ile Asp Lys Ile
675 680 685
Glu Phe Ile Pro Ile Glu Gly Ser Leu Glu Glu Tyr Lys Ala Asp Gln
690 695 700
Val Leu Glu Lys Ala Lys Lys Ala Val Asn Thr Leu Phe Thr Asn Asp
705 710 715 720
Thr Lys Thr Thr Leu Lys Leu Asn Val Thr Asp Tyr Ala Val Asp Gln
725 730 735
Ala Ala Asn Leu Val Glu Cys Ile Ser Glu Asp Phe His Ala Gln Glu
740 745 750
Lys Met Ile Leu Leu Asp Gln Ile Arg Val Ala Lys Arg Leu Ser Gln
755 760 765
Ala Arg Asn Leu Leu Asn Tyr Gly Asp Phe Glu Ser Pro Asp Trp Ser
770 775 780
Gly Glu Asn Gly Trp Lys Thr Ser His His Val Ser Val Arg Ala Asp
785 790 795 800
Asn Pro Ile Phe Lys Gly Arg Tyr Leu His Met Leu Gly Ala Thr Ser
805 810 815
Ser Gln Phe Ser Asn Asn Val Tyr Pro Thr Tyr Val Tyr Gln Lys Val
820 825 830
Asp Glu Ser Lys Leu Glu Ser Tyr Thr Arg Tyr Leu Ile Arg Gly Phe
835 840 845
Val Gly Asn Ser Lys Asp Leu Glu Leu Leu Val Glu Arg Tyr Gly Gln
850 855 860
Asp Val His Val Glu Met Gly Val Pro Asn Asp Ile His Tyr Ser Leu
865 870 875 880
Pro Met Asn Glu Cys Gly Gly Phe Asn Arg Cys Gly Pro Thr Leu Ser
885 890 895
Gln Thr Arg Ser Asp His Thr Cys Thr Cys Asn Asp Met Ala Ser Met
900 905 910
His Thr Asp Cys Gln Cys Gln Asn Lys Ala Asn Arg Ile Ala Thr Gly
915 920 925
Val Tyr Thr Asn Gly Phe His Ala His Lys Ser Cys Gly Cys Lys Asn
930 935 940
Asn Asp Met Tyr Gln Arg Gly Thr Tyr Pro His Lys Pro Cys Gly Cys
945 950 955 960
Lys Asp Pro His Val Phe Thr Tyr His Ile Asp Thr Gly Cys Val Asp
965 970 975
Gln Glu Glu Asn Leu Gly Leu Phe Phe Ala Leu Lys Ile Ala Ser Glu
980 985 990
Asn Gly Val Ala Asn Ile Asp Asn Leu Glu Ile Ile Glu Ala Gln Pro
995 1000 1005
Leu Thr Gly Glu Ala Leu Ala Arg Val Lys Lys Arg Glu Gln Arg
1010 1015 1020
Trp Lys Gln Glu Arg Asp Lys Lys Arg Leu Glu Ile Glu Lys Ala
1025 1030 1035
Val Gln Ala Ala Gln Asp Ala Ile Gln Lys Leu Phe Thr Asn Thr
1040 1045 1050
Gln Gln Asn Arg Leu Lys Phe Glu Thr Leu Phe Pro Gln Ile Val
1055 1060 1065
His Ala Glu Lys Leu Val Gln Gln Ile Pro Tyr Val Tyr His Pro
1070 1075 1080
Phe Leu Ser Glu Ala Leu Pro Thr Val Pro Gly Met Asn Phe Glu
1085 1090 1095
Ile Ile Arg Gln Leu Ser Val Met Ile Gly Asn Ala Arg Ala Leu
1100 1105 1110
Tyr Glu Gly Arg Asn Leu Val His Asn Gly Thr Phe Ser Ser Gly
1115 1120 1125
Thr Gly Asn Trp Asn Val Ser Glu Gly Gly Lys Val Gln Ser Leu
1130 1135 1140
Gln Asn Thr Ser Val Leu Val Leu Ser Glu Trp Asn His Glu Ala
1145 1150 1155
Ser Gln Gln Leu His Ile Asp Pro Asp Arg Gly Tyr Val Leu Arg
1160 1165 1170
Val Thr Ala Arg Lys Glu Asp Gly Gly Lys Gly Thr Val Thr Met
1175 1180 1185
Ser Asp Cys Ala Ala Tyr Thr Glu Ala Leu Thr Phe Thr Ser Cys
1190 1195 1200
Asp Tyr Asn Thr Leu Gly Ser Gln Lys Met Thr Ser Gly Thr Leu
1205 1210 1215
Ser Gly Phe Met Thr Lys Thr Leu Glu Ile Phe Pro Asp Thr Asp
1220 1225 1230
Arg Ile Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr Phe Gln Val
1235 1240 1245
Glu Ser Val Glu Leu Ile Cys Met Glu His Met Glu Asp Pro Met
1250 1255 1260
Tyr Asp Val Ala Gly Asn Val Glu Glu Glu Leu His Tyr Leu Lys
1265 1270 1275
Glu Pro Gly Ser Ile Arg Ser Thr Val Val Ser Asp Pro Leu Cys
1280 1285 1290
Tyr Thr Lys Ile Gly Glu Phe Gly Cys Pro Leu Asn Gln
1295 1300 1305
<210> 18
<211> 1275
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 18
Met Asn Gln Asn Tyr Asn Asn Lys Glu Tyr Glu Ile Leu Ser Thr Gly
1 5 10 15
Gly Lys Asp Tyr Gln Pro Arg Tyr Pro Leu Ala Gln Glu Pro Asn Ala
20 25 30
Glu Leu Gln Gln Met Ser Tyr Lys Asp Trp Met Asn Arg Cys Ala Gly
35 40 45
Val Thr Ser Gly Glu Leu Phe Asp Ala Asp Ala Val Lys Thr Ala Leu
50 55 60
Ile Ile Gly Thr Gly Ile Ser Trp Ala Ile Leu Gly Val Ala Phe Pro
65 70 75 80
Pro Leu Ser Val Ala Val Gly Val Leu Asn Ile Ile Ile Ala Tyr Leu
85 90 95
Trp Pro Ser Gln Ala Thr Glu Asp Gln Phe Ser Trp Lys Gln Met Met
100 105 110
Ser Ala Thr Glu Gln Leu Ile Asn Gln Ala Ile Asn Pro Ile Ile Lys
115 120 125
Ser Lys Ala Ile Asp Ala Leu Arg Asp Leu Gln Ser Ser Leu Arg Asp
130 135 140
Tyr Asn Gln Ala Leu Cys Asn Leu Gln Thr Asn Leu Asp Ser Pro Asp
145 150 155 160
Val Glu Arg Tyr Lys Glu Val Val Arg Arg Glu Phe Asn Asp Ala Asn
165 170 175
Asp Lys Ala Lys Ser Thr Ile Ile Asn Leu Glu Ser Asp Gly Phe Gln
180 185 190
Ile Pro Phe Leu Ser Ser Tyr Ala Gln Ala Ala Asn Ile His Leu Leu
195 200 205
Leu Leu Lys Asp Val Lys Gln Tyr Gly Glu Ile Trp Gly Leu Thr Ala
210 215 220
Leu Glu Val Glu Gln Tyr Phe Asp Asn Pro Pro Ser Lys Pro Gly Asn
225 230 235 240
Pro Gly Met Ile Gln Leu Ile Gly Ile Tyr Thr Asp Tyr Cys Ile His
245 250 255
Trp Tyr Asn Arg Gly Leu Gln Gln Gln Ala Asp Arg Tyr Tyr Trp Ser
260 265 270
Ser Phe Asn Asp Phe Arg Arg Thr Met Thr Ile Met Val Leu Asp Ile
275 280 285
Val Ala Leu Trp Pro Thr Tyr Asn Pro Ile Asn Tyr Pro Lys Pro Val
290 295 300
Lys Thr Gln Leu Thr Arg Lys Val Tyr Thr Pro Ala Phe Gly Gln Pro
305 310 315 320
Ile Tyr Ser Ser Glu Trp Leu Phe Asn Glu Leu Glu Leu Ser Leu Val
325 330 335
Thr Pro Pro Arg Leu Phe Thr Phe Leu Asp Ser Val Thr Phe Tyr Arg
340 345 350
Arg Asp Asp Glu Leu Thr Gln Tyr Ala Gly Ile Met Gln Asn Leu Arg
355 360 365
Tyr Thr Ser Ser Leu Lys Leu Phe Tyr Thr Leu Gln Gly Gln Arg Thr
370 375 380
Asp Leu Val Asp Ser Ile Arg Ile Tyr Ser Gly Val Phe Asp Glu Val
385 390 395 400
Trp Lys Thr Ala Ser Val Val Gly Glu Phe Glu Asn Ala Gly Gly Thr
405 410 415
Gln Leu Gln Thr Phe Ser Phe Tyr Leu Leu Ser Asn Pro Glu Asn Pro
420 425 430
Val Thr Ile Gly His Val Tyr Ser Ser Asn Thr Thr Ala Ala Thr Tyr
435 440 445
Gly Phe Pro Cys Val Ala Thr Asn Ile Ser Cys Ile Gly Glu Pro Cys
450 455 460
Thr Pro Cys Ser Ile Ser Gln Pro Leu Glu Ser Ile Cys Asn Ile Pro
465 470 475 480
Gly Lys Ser Ser His Phe Leu Ala Ser Val Thr Ala Asp Val Tyr Gly
485 490 495
His Val Arg Ile Ala Ser Tyr Gly Trp Asn His Ile Ser Ala Asp Thr
500 505 510
Val Asn Leu Leu Asn Ala Glu Lys Ile Thr Gln Ile Pro Ala Val Lys
515 520 525
Gly Asn Thr Met Ile Gly Asp Ser Ser Val Val Lys Gly Pro Gly Ser
530 535 540
Thr Gly Gly Asp Leu Val Lys Met Ser Glu Arg Ala Val Phe Ser Leu
545 550 555 560
Gly Leu Thr Ile Pro Ala Lys Asn Glu Arg Leu Asn Gly Tyr Ala Ile
565 570 575
Arg Leu Arg Tyr Thr Ser Asn Ala Asp Ile Asn Phe Gln Ile Phe Ile
580 585 590
Arg Tyr Asn Ser Gln Lys Thr Val Val Ser Asn Gln Ile Ile Pro Pro
595 600 605
Thr Ser Phe Asp Asn Ser Leu Thr Tyr Gln Thr Phe Gly Tyr Tyr Thr
610 615 620
Leu Ser Glu Leu Glu Leu Glu Thr Leu Ser Glu Val Thr Pro Glu Ala
625 630 635 640
Val Ile Thr Ile Asp Lys Phe Gly Ala Gly Asp Ile Leu Ile Asp Lys
645 650 655
Ile Glu Phe Ile Pro Ile Glu Gly Ser Leu Glu Glu Tyr Lys Ala Asp
660 665 670
Gln Val Leu Glu Lys Ala Lys Lys Ala Val Asn Thr Leu Phe Thr Asn
675 680 685
Asp Thr Lys Thr Thr Leu Lys Leu Asn Val Thr Asp Tyr Ala Val Asp
690 695 700
Gln Ala Ala Asn Leu Val Glu Cys Ile Ser Glu Asp Phe His Ala Gln
705 710 715 720
Glu Lys Met Ile Leu Leu Asp Gln Ile Arg Val Ala Lys Arg Leu Ser
725 730 735
Gln Ala Arg Asn Leu Leu Asn Tyr Gly Asp Phe Glu Ser Pro Asp Trp
740 745 750
Ser Gly Glu Asn Gly Trp Lys Thr Ser His His Val Ser Val Arg Ala
755 760 765
Asp Asn Pro Ile Phe Lys Gly Arg Tyr Leu His Met Leu Gly Ala Thr
770 775 780
Ser Ser Gln Phe Ser Asn Asn Val Tyr Pro Thr Tyr Val Tyr Gln Lys
785 790 795 800
Val Asp Glu Ser Lys Leu Glu Ser Tyr Thr Arg Tyr Leu Ile Arg Gly
805 810 815
Phe Val Gly Asn Ser Lys Asp Leu Glu Leu Leu Val Glu Arg Tyr Gly
820 825 830
Gln Asp Val His Val Glu Met Gly Val Pro Asn Asp Ile His Tyr Ser
835 840 845
Leu Pro Met Asn Glu Cys Gly Gly Phe Asn Arg Cys Gly Pro Thr Leu
850 855 860
Ser Gln Thr Arg Ser Asp His Thr Cys Thr Cys Asn Asp Met Ala Ser
865 870 875 880
Met His Thr Asp Cys Gln Cys Gln Asn Lys Ala Asn Arg Ile Ala Thr
885 890 895
Gly Val Tyr Thr Asn Gly Phe His Ala His Lys Ser Cys Gly Cys Lys
900 905 910
Asn Asn Asp Met Tyr Gln Arg Gly Thr Tyr Pro His Lys Pro Cys Gly
915 920 925
Cys Lys Asp Pro His Val Phe Thr Tyr His Ile Asp Thr Gly Cys Val
930 935 940
Asp Gln Glu Glu Asn Leu Gly Leu Phe Phe Ala Leu Lys Ile Ala Ser
945 950 955 960
Glu Asn Gly Val Ala Asn Ile Asp Asn Leu Glu Ile Ile Glu Ala Gln
965 970 975
Pro Leu Thr Gly Glu Ala Leu Ala Arg Val Lys Lys Arg Glu Gln Arg
980 985 990
Trp Lys Gln Glu Arg Asp Lys Lys Arg Leu Glu Ile Glu Lys Ala Val
995 1000 1005
Gln Ala Ala Gln Asp Ala Ile Gln Lys Leu Phe Thr Asn Thr Gln
1010 1015 1020
Gln Asn Arg Leu Lys Phe Glu Thr Leu Phe Pro Gln Ile Val His
1025 1030 1035
Ala Glu Lys Leu Val Gln Gln Ile Pro Tyr Val Tyr His Pro Phe
1040 1045 1050
Leu Ser Glu Ala Leu Pro Thr Val Pro Gly Met Asn Phe Glu Ile
1055 1060 1065
Ile Arg Gln Leu Ser Val Met Ile Gly Asn Ala Arg Ala Leu Tyr
1070 1075 1080
Glu Gly Arg Asn Leu Val His Asn Gly Thr Phe Ser Ser Gly Thr
1085 1090 1095
Gly Asn Trp Asn Val Ser Glu Gly Gly Lys Val Gln Ser Leu Gln
1100 1105 1110
Asn Thr Ser Val Leu Val Leu Ser Glu Trp Asn His Glu Ala Ser
1115 1120 1125
Gln Gln Leu His Ile Asp Pro Asp Arg Gly Tyr Val Leu Arg Val
1130 1135 1140
Thr Ala Arg Lys Glu Asp Gly Gly Lys Gly Thr Val Thr Met Ser
1145 1150 1155
Asp Cys Ala Ala Tyr Thr Glu Ala Leu Thr Phe Thr Ser Cys Asp
1160 1165 1170
Tyr Asn Thr Leu Gly Ser Gln Lys Met Thr Ser Gly Thr Leu Ser
1175 1180 1185
Gly Phe Met Thr Lys Thr Leu Glu Ile Phe Pro Asp Thr Asp Arg
1190 1195 1200
Ile Arg Ile Asp Ile Gly Glu Thr Glu Gly Thr Phe Gln Val Glu
1205 1210 1215
Ser Val Glu Leu Ile Cys Met Glu His Met Glu Asp Pro Met Tyr
1220 1225 1230
Asp Val Ala Gly Asn Val Glu Glu Glu Leu His Tyr Leu Lys Glu
1235 1240 1245
Pro Gly Ser Ile Arg Ser Thr Val Val Ser Asp Pro Leu Cys Tyr
1250 1255 1260
Thr Lys Ile Gly Glu Phe Gly Cys Pro Leu Asn Gln
1265 1270 1275
<210> 19
<211> 662
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 19
Met Asn Gln Asn Tyr Asn Asn Lys Glu Tyr Glu Ile Leu Ser Thr Gly
1 5 10 15
Gly Lys Asp Tyr Gln Pro Arg Tyr Pro Leu Ala Gln Glu Pro Asn Ala
20 25 30
Glu Leu Gln Gln Met Ser Tyr Lys Asp Trp Met Asn Arg Cys Ala Gly
35 40 45
Val Thr Ser Gly Glu Leu Phe Asp Ala Asp Ala Val Lys Thr Ala Leu
50 55 60
Ile Ile Gly Thr Gly Ile Ser Trp Ala Ile Leu Gly Val Ala Phe Pro
65 70 75 80
Pro Leu Ser Val Ala Val Gly Val Leu Asn Ile Ile Ile Ala Tyr Leu
85 90 95
Trp Pro Ser Gln Ala Thr Glu Asp Gln Phe Ser Trp Lys Gln Met Met
100 105 110
Ser Ala Thr Glu Gln Leu Ile Asn Gln Ala Ile Asn Pro Ile Ile Lys
115 120 125
Ser Lys Ala Ile Asp Ala Leu Arg Asp Leu Gln Ser Ser Leu Arg Asp
130 135 140
Tyr Asn Gln Ala Leu Cys Asn Leu Gln Thr Asn Leu Asp Ser Pro Asp
145 150 155 160
Val Glu Arg Tyr Lys Glu Val Val Arg Arg Glu Phe Asn Asp Ala Asn
165 170 175
Asp Lys Ala Lys Ser Thr Ile Ile Asn Leu Glu Ser Asp Gly Phe Gln
180 185 190
Ile Pro Phe Leu Ser Ser Tyr Ala Gln Ala Ala Asn Ile His Leu Leu
195 200 205
Leu Leu Lys Asp Val Lys Gln Tyr Gly Glu Ile Trp Gly Leu Thr Ala
210 215 220
Leu Glu Val Glu Gln Tyr Phe Asp Asn Pro Pro Ser Lys Pro Gly Asn
225 230 235 240
Pro Gly Met Ile Gln Leu Ile Gly Ile Tyr Thr Asp Tyr Cys Ile His
245 250 255
Trp Tyr Asn Arg Gly Leu Gln Gln Gln Ala Asp Arg Tyr Tyr Trp Ser
260 265 270
Ser Phe Asn Asp Phe Arg Arg Thr Met Thr Ile Met Val Leu Asp Ile
275 280 285
Val Ala Leu Trp Pro Thr Tyr Asn Pro Ile Asn Tyr Pro Lys Pro Val
290 295 300
Lys Thr Gln Leu Thr Arg Lys Val Tyr Thr Pro Ala Phe Gly Gln Pro
305 310 315 320
Ile Tyr Ser Ser Glu Trp Leu Phe Asn Glu Leu Glu Leu Ser Leu Val
325 330 335
Thr Pro Pro Arg Leu Phe Thr Phe Leu Asp Ser Val Thr Phe Tyr Arg
340 345 350
Arg Asp Asp Glu Leu Thr Gln Tyr Ala Gly Ile Met Gln Asn Leu Arg
355 360 365
Tyr Thr Ser Ser Leu Lys Leu Phe Tyr Thr Leu Gln Gly Gln Arg Thr
370 375 380
Asp Leu Val Asp Ser Ile Arg Ile Tyr Ser Gly Val Phe Asp Glu Val
385 390 395 400
Trp Lys Thr Ala Ser Val Val Gly Glu Phe Glu Asn Ala Gly Gly Thr
405 410 415
Gln Leu Gln Thr Phe Ser Phe Tyr Leu Leu Ser Asn Pro Glu Asn Pro
420 425 430
Val Thr Ile Gly His Val Tyr Ser Ser Asn Thr Thr Ala Ala Thr Tyr
435 440 445
Gly Phe Pro Cys Val Ala Thr Asn Ile Ser Cys Ile Gly Glu Pro Cys
450 455 460
Thr Pro Cys Ser Ile Ser Gln Pro Leu Glu Ser Ile Cys Asn Ile Pro
465 470 475 480
Gly Lys Ser Ser His Phe Leu Ala Ser Val Thr Ala Asp Val Tyr Gly
485 490 495
His Val Arg Ile Ala Ser Tyr Gly Trp Asn His Ile Ser Ala Asp Thr
500 505 510
Val Asn Leu Leu Asn Ala Glu Lys Ile Thr Gln Ile Pro Ala Val Lys
515 520 525
Gly Asn Thr Met Ile Gly Asp Ser Ser Val Val Lys Gly Pro Gly Ser
530 535 540
Thr Gly Gly Asp Leu Val Lys Met Ser Glu Arg Ala Val Phe Ser Leu
545 550 555 560
Gly Leu Thr Ile Pro Ala Lys Asn Glu Arg Leu Asn Gly Tyr Ala Ile
565 570 575
Arg Leu Arg Tyr Thr Ser Asn Ala Asp Ile Asn Phe Gln Ile Phe Ile
580 585 590
Arg Tyr Asn Ser Gln Lys Thr Val Val Ser Asn Gln Ile Ile Pro Pro
595 600 605
Thr Ser Phe Asp Asn Ser Leu Thr Tyr Gln Thr Phe Gly Tyr Tyr Thr
610 615 620
Leu Ser Glu Leu Glu Leu Glu Thr Leu Ser Glu Val Thr Pro Glu Ala
625 630 635 640
Val Ile Thr Ile Asp Lys Phe Gly Ala Gly Asp Ile Leu Ile Asp Lys
645 650 655
Ile Glu Phe Ile Pro Ile
660
<210> 20
<211> 693
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 20
Leu Arg Ile Lys Tyr Ala Tyr Asn Leu His His Leu Ile Ile Asn Asn
1 5 10 15
His Tyr Asn Glu Glu Lys Ser Pro Leu Gln Asn Gly Gly Ser Tyr Met
20 25 30
Asn Gln Asn Tyr Asn Asn Lys Glu Tyr Glu Ile Leu Ser Thr Gly Gly
35 40 45
Lys Asp Tyr Gln Pro Arg Tyr Pro Leu Ala Gln Glu Pro Asn Ala Glu
50 55 60
Leu Gln Gln Met Ser Tyr Lys Asp Trp Met Asn Arg Cys Ala Gly Val
65 70 75 80
Thr Ser Gly Glu Leu Phe Asp Ala Asp Ala Val Lys Thr Ala Leu Ile
85 90 95
Ile Gly Thr Gly Ile Ser Trp Ala Ile Leu Gly Val Ala Phe Pro Pro
100 105 110
Leu Ser Val Ala Val Gly Val Leu Asn Ile Ile Ile Ala Tyr Leu Trp
115 120 125
Pro Ser Gln Ala Thr Glu Asp Gln Phe Ser Trp Lys Gln Met Met Ser
130 135 140
Ala Thr Glu Gln Leu Ile Asn Gln Ala Ile Asn Pro Ile Ile Lys Ser
145 150 155 160
Lys Ala Ile Asp Ala Leu Arg Asp Leu Gln Ser Ser Leu Arg Asp Tyr
165 170 175
Asn Gln Ala Leu Cys Asn Leu Gln Thr Asn Leu Asp Ser Pro Asp Val
180 185 190
Glu Arg Tyr Lys Glu Val Val Arg Arg Glu Phe Asn Asp Ala Asn Asp
195 200 205
Lys Ala Lys Ser Thr Ile Ile Asn Leu Glu Ser Asp Gly Phe Gln Ile
210 215 220
Pro Phe Leu Ser Ser Tyr Ala Gln Ala Ala Asn Ile His Leu Leu Leu
225 230 235 240
Leu Lys Asp Val Lys Gln Tyr Gly Glu Ile Trp Gly Leu Thr Ala Leu
245 250 255
Glu Val Glu Gln Tyr Phe Asp Asn Pro Pro Ser Lys Pro Gly Asn Pro
260 265 270
Gly Met Ile Gln Leu Ile Gly Ile Tyr Thr Asp Tyr Cys Ile His Trp
275 280 285
Tyr Asn Arg Gly Leu Gln Gln Gln Ala Asp Arg Tyr Tyr Trp Ser Ser
290 295 300
Phe Asn Asp Phe Arg Arg Thr Met Thr Ile Met Val Leu Asp Ile Val
305 310 315 320
Ala Leu Trp Pro Thr Tyr Asn Pro Ile Asn Tyr Pro Lys Pro Val Lys
325 330 335
Thr Gln Leu Thr Arg Lys Val Tyr Thr Pro Ala Phe Gly Gln Pro Ile
340 345 350
Tyr Ser Ser Glu Trp Leu Phe Asn Glu Leu Glu Leu Ser Leu Val Thr
355 360 365
Pro Pro Arg Leu Phe Thr Phe Leu Asp Ser Val Thr Phe Tyr Arg Arg
370 375 380
Asp Asp Glu Leu Thr Gln Tyr Ala Gly Ile Met Gln Asn Leu Arg Tyr
385 390 395 400
Thr Ser Ser Leu Lys Leu Phe Tyr Thr Leu Gln Gly Gln Arg Thr Asp
405 410 415
Leu Val Asp Ser Ile Arg Ile Tyr Ser Gly Val Phe Asp Glu Val Trp
420 425 430
Lys Thr Ala Ser Val Val Gly Glu Phe Glu Asn Ala Gly Gly Thr Gln
435 440 445
Leu Gln Thr Phe Ser Phe Tyr Leu Leu Ser Asn Pro Glu Asn Pro Val
450 455 460
Thr Ile Gly His Val Tyr Ser Ser Asn Thr Thr Ala Ala Thr Tyr Gly
465 470 475 480
Phe Pro Cys Val Ala Thr Asn Ile Ser Cys Ile Gly Glu Pro Cys Thr
485 490 495
Pro Cys Ser Ile Ser Gln Pro Leu Glu Ser Ile Cys Asn Ile Pro Gly
500 505 510
Lys Ser Ser His Phe Leu Ala Ser Val Thr Ala Asp Val Tyr Gly His
515 520 525
Val Arg Ile Ala Ser Tyr Gly Trp Asn His Ile Ser Ala Asp Thr Val
530 535 540
Asn Leu Leu Asn Ala Glu Lys Ile Thr Gln Ile Pro Ala Val Lys Gly
545 550 555 560
Asn Thr Met Ile Gly Asp Ser Ser Val Val Lys Gly Pro Gly Ser Thr
565 570 575
Gly Gly Asp Leu Val Lys Met Ser Glu Arg Ala Val Phe Ser Leu Gly
580 585 590
Leu Thr Ile Pro Ala Lys Asn Glu Arg Leu Asn Gly Tyr Ala Ile Arg
595 600 605
Leu Arg Tyr Thr Ser Asn Ala Asp Ile Asn Phe Gln Ile Phe Ile Arg
610 615 620
Tyr Asn Ser Gln Lys Thr Val Val Ser Asn Gln Ile Ile Pro Pro Thr
625 630 635 640
Ser Phe Asp Asn Ser Leu Thr Tyr Gln Thr Phe Gly Tyr Tyr Thr Leu
645 650 655
Ser Glu Leu Glu Leu Glu Thr Leu Ser Glu Val Thr Pro Glu Ala Val
660 665 670
Ile Thr Ile Asp Lys Phe Gly Ala Gly Asp Ile Leu Ile Asp Lys Ile
675 680 685
Glu Phe Ile Pro Ile
690
<210> 21
<211> 296
<212> БЕЛОК
<213> Bacillus sp.
<400> 21
Met Ala Ile Val Asp Leu Asp Ala Tyr Leu Leu Ala Leu Ala Lys Lys
1 5 10 15
Ala His Pro Phe Gly Ser Leu Phe His Ala Val Ser Thr Arg Gln Leu
20 25 30
Ser Val Glu Asn Ala Glu Ser Tyr Gly Phe Arg Ile Glu Asn Ser Lys
35 40 45
Pro Gln Gly Thr Met Phe Met Gly Glu Ser Glu Leu Lys Asn Asp Thr
50 55 60
Asn Glu Thr Gln Thr Ile His Ser Asp Ser Phe Thr Lys Thr Ile Thr
65 70 75 80
Asp Ser Val Thr Leu Ser Val Thr Asn Gly Val Thr Ala Gly Val Ser
85 90 95
Val Thr Ile Ser Gly Lys Ile Phe Gly Ile Gly Ala Glu Ser Ser Met
100 105 110
Ser Phe Glu Val Ser Thr Ser Thr Thr Asn Glu Gln Thr Ser Glu Glu
115 120 125
Ser Val Ala Tyr Thr Val Pro Ser Gln Gly Val Val Val Pro Ala Arg
130 135 140
Lys Thr Tyr Tyr Val Tyr Ser Ala Leu Gln Arg Ser Leu Leu Glu Gly
145 150 155 160
Ser Ile Arg Leu Arg Ala Asp Leu Ser Gly Glu Val Tyr Ala Ser Met
165 170 175
Lys Val Gly Gly Ala Ile Gly Ser Ile Pro Leu Gly Asp Ile Tyr Glu
180 185 190
Phe Ile Lys Thr His Gln Leu Leu His Pro Leu Pro Ser Gly Ile Ser
195 200 205
Leu Asn His Asn Asn Lys Ser Ile His Phe Glu Gly Val Ala Gln Tyr
210 215 220
Leu Tyr Gly Thr Gly Thr Lys Phe Asn Val Thr Ile Thr Asp Thr Pro
225 230 235 240
Ser Ser Gln Gly Thr Gln Glu His Lys Ala Phe Asp Ala Lys Thr Gly
245 250 255
Leu Gly Thr Tyr Glu Ile Gln Leu Asp Gly Arg Lys Leu Gly Phe Asp
260 265 270
Leu Asn Glu Leu Lys Asp Lys Met Glu Pro Glu Val Phe Glu Lys Leu
275 280 285
Lys Glu Leu Gln Asn Glu Ile Val
290 295
<210> 22
<211> 266
<212> БЕЛОК
<213> Streptomyces sp.
<400> 22
Val Pro Val Arg Val Arg Arg Ala Ser Pro Arg Pro Ala Ser Pro Ala
1 5 10 15
Ala Pro Gly Val Arg Ala Gly Ala Ala Pro Ala Gly Ser Pro Ala Ser
20 25 30
Gly Pro Glu Pro Leu Leu Pro Val Val Pro Gly Arg Pro Val Arg Thr
35 40 45
Ala Asp Gly Ala Arg Ser Asp Gly Arg Ala Leu Gly Ile Leu Thr Pro
50 55 60
Pro Asp His Arg Asp His Thr Gly Gly Ala Leu Ala Ala Ser Phe Arg
65 70 75 80
Thr Val Val Glu Val Gly Pro Asp His Leu Ala Gln Ala Arg Ser Ile
85 90 95
Asp Arg Val Phe Gln Glu Ala Ile Ala Pro Ala Thr Val Asn Phe Asp
100 105 110
Phe Glu Ala Ile Arg Glu Ala Ala Ala Ala Val Pro Asp Ser Thr Val
115 120 125
Val Lys Met Val Arg Gly Trp Gly Leu Gln Glu Tyr Ala Pro Val Leu
130 135 140
Val Met Val Leu Ser Leu Lys Glu Ala Val Arg Gln Ala Leu Pro Pro
145 150 155 160
Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr Val Glu Arg Glu Leu Val
165 170 175
Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu Gly Gln Pro Gly Leu Ser
180 185 190
Phe Tyr Glu Glu Ser Gly Glu Arg Thr Arg Phe Tyr Arg Asp Leu Phe
195 200 205
Phe Ala Leu Gln Asp Glu Glu Thr Gly Glu His Met Tyr Ala Leu Ala
210 215 220
Phe Cys Val Asp Val Thr Val Glu Leu Pro Lys Ala Arg Ala Val Ala
225 230 235 240
Leu Glu Leu Thr Asp Ile Val Pro Phe Ala Val Arg Leu Asn Ala Ile
245 250 255
Val Val Arg Gln Ala Leu Asp Leu Val Ala
260 265
<210> 23
<211> 312
<212> БЕЛОК
<213> Bacillus sp.
<400> 23
Met Ala Ile Thr Asp Ile Arg Leu Ala Ser Glu Asp Tyr Leu Arg Phe
1 5 10 15
His Ala Gln His Tyr Trp Gly Gly Ala Thr Val Leu Ser Thr Gly Phe
20 25 30
Leu Pro Tyr Leu Ile Gln Asn Pro Ile Ala Val Pro Asp Gln Thr Val
35 40 45
Phe Glu Thr Val Pro Val Gln Lys Val Ser Ser Val Gln Val Leu Thr
50 55 60
Asn Asn Thr Asp Val Glu Gln Ser Gln Thr Val Lys Phe Ser Glu Lys
65 70 75 80
Val Ile Glu Thr Thr Thr Ser Ser Thr Thr Glu Gly Tyr Lys Ile Gly
85 90 95
Ser Gly Ile Thr Ser Thr Ser Lys Phe Gly Tyr Ser Leu Lys Val Lys
100 105 110
Gly Asn Ser Ser Gly Phe Asp Gln Thr Phe Thr Leu Thr Thr Thr Ser
115 120 125
Glu Tyr Asn His Ser Ser Thr Glu Thr Lys Thr Gln Thr Thr Glu Arg
130 135 140
Leu Trp Glu Leu Thr Gln Pro Val Arg Val Ala Pro His Thr Gln Val
145 150 155 160
Val Thr Thr Leu Thr Ile Phe Gly Gly Glu Ala Arg Ile Pro Met Thr
165 170 175
Leu Asn Ala Asn Leu Leu Gly Gln Gly Tyr Gly Gln Glu Ala Tyr Ser
180 185 190
Glu Leu Asn Leu Arg Lys Thr Asp Gly Ser Val Trp Tyr Asn Trp Ile
195 200 205
Pro Ala Ser Asn Met Tyr Asp Thr Asn Trp Pro Asn Lys Pro Ala Ser
210 215 220
Phe Ala Gly Tyr Thr Gly Arg Ser Leu Ile Leu Lys Gly Ala Ser Val
225 230 235 240
Thr Thr Thr Gly Pro Cys Leu Tyr Ser Thr Val Arg Phe Asp Glu Thr
245 250 255
Pro Leu Pro Gly Tyr Glu Lys Asp Ser Gln Pro Arg Ser Trp Tyr Ser
260 265 270
Asn Glu Val Leu Leu Arg Asp Gly Thr Ile Ile Thr Ile Pro Asp Leu
275 280 285
Asp Pro Lys Thr Gly Arg Ala Leu Pro Pro Lys Arg Pro Glu Gly Ile
290 295 300
Leu Val Ser Ser Arg Ile Thr Asp
305 310
<210> 24
<211> 966
<212> БЕЛОК
<213> Bacillus sp.
<400> 24
Val Met Lys Leu Val Ser Asn Ser Met Val Pro Tyr Asn Val Leu Arg
1 5 10 15
Asn Met Asn Met Pro Thr Ile Ser Gly Thr Arg Trp Asn Lys Glu Met
20 25 30
Phe Ser Ser Ala Leu Asp Asn Thr Ser Val Leu Leu Asp Leu Ile Glu
35 40 45
Lys Gly Ile Asn Asp Gly Asp Asp Val Leu Gly Val Leu Ser Phe Ile
50 55 60
Gly Leu Thr Ala Leu Glu Ala Ile Pro Val Val Gly Gly Val Leu Ser
65 70 75 80
Ser Leu Val Ser Met Leu Phe Phe Pro Thr Lys Ser Ser Ile Asp Phe
85 90 95
Lys Lys Ile Trp Glu Asn Leu Glu Lys Ala Ile Asn Gln Ile Val Asp
100 105 110
Lys Lys Ile Glu Glu Ala Leu Met Ser Gln Leu Met Gln Glu Ile Ala
115 120 125
Gly Leu Ala Asn Val Leu Lys Glu Tyr Arg Asp Ala Tyr Asp Ser Tyr
130 135 140
Asn Gly Lys Lys Val Phe Asp Ile Pro Asp Arg Leu Thr Pro Glu Glu
145 150 155 160
Tyr Leu Val Gln Val Phe Thr Ser Ala Asn Leu Leu Phe Ile Gln Arg
165 170 175
Ile Pro Thr Phe Gln Asn Pro Lys Tyr Asp Val Leu Phe Leu Pro Phe
180 185 190
Phe Val His Val Ala Glu Met His Ile Leu Leu Ile Arg Asp Ala Ala
195 200 205
Ile His Gly Leu Glu Trp Gly Met Asp Glu Glu Ile His Gln Lys Phe
210 215 220
Lys Arg Asn Leu Lys Glu Ile Ile Asp Glu Tyr Thr Ser Tyr Leu Leu
225 230 235 240
Lys Thr Tyr Asn Lys Gly Leu Lys Glu Val Ser Glu Arg Glu Leu Asn
245 250 255
Asp Lys Asp Phe Pro Thr Thr Asn Asn Lys Asn His Tyr Ile Asn Thr
260 265 270
Val Arg Trp Asn Val Ile Asn Gln Tyr Lys Arg Gly Met Thr Leu Thr
275 280 285
Ala Phe Asp Phe Ala Tyr Lys Trp Lys Tyr Tyr His Glu Val Tyr Gln
290 295 300
Asn Asn Ile Thr Leu Asn Pro Phe Arg Thr Ile Tyr Ser Asp Ile Ala
305 310 315 320
Gly Ser Val Tyr Pro Tyr Ile Lys Thr Thr Asn Glu Ile Asn Asn Thr
325 330 335
Ile Lys Asn Gln Asn Leu Lys Tyr Arg Gly Ile Leu Lys Arg Leu Gln
340 345 350
Val Tyr His Ala Glu Arg Ile Asp Ser Ile Gln Ser Thr Tyr Ile Arg
355 360 365
Asn Asn Glu Ile Ile Asn Ser Thr Lys Ile Gly Gly Val Gly Gly Arg
370 375 380
Ile Ser Ser Leu Val Leu Glu Asp Pro Ile Lys Asn Pro Leu Ile Gln
385 390 395 400
Val Asn Met Trp Ser Glu Leu Val Pro Ile Ser Leu Gly Phe Lys Phe
405 410 415
Tyr Asp Glu Lys Glu Gln Asn Ile Gly Gly Gly Ser Trp Trp Pro Lys
420 425 430
Pro His Lys Phe Gly Ala Tyr His Phe Val Gly Asn Lys Val Ser Ser
435 440 445
Ile Ile Gly Phe Gly Lys Asn Glu Thr Gly Gly Phe Asn Ser Leu Asp
450 455 460
Ala Met Ile Val Gly Phe Lys Arg Asp Asp Tyr Thr Thr Glu Asn Arg
465 470 475 480
Leu Leu Gly Ile Asn Glu Thr Gly Glu Pro Val Thr Lys Val Ile Asp
485 490 495
Ala Gly Asn Phe His Gln Glu Lys Phe Gly Ser Asn Ile Gln Ile Thr
500 505 510
Asp Glu Pro Met Phe Gly Asp Gly Val Leu Gln Phe Thr Asn Tyr Ala
515 520 525
Asn Thr Met Asn Gln Asp Ser Ser Val Thr Tyr Gln Ile Asn Thr Glu
530 535 540
Ile Glu Gly Ile Tyr Gln Leu His Ala Ile Ile Gly Ala Lys Lys Gln
545 550 555 560
Lys Asp Lys Leu Ser Phe Lys Val Ile Leu Asn Thr Lys Gln Gln Lys
565 570 575
Lys Ile Ile Thr Asp Ser Phe Asp Ser His Asp Ile Trp Glu Gly Met
580 585 590
Ser Leu Asn Glu Ser Ile Val Tyr Lys Arg Ile Leu Ile Gly Thr Phe
595 600 605
Pro Leu Lys Arg Gly Met Asn Ser Ile Thr Ile His Asn Asp Val Leu
610 615 620
Gln Ser Ser Ala Asn Ile Lys Thr Trp Asn Leu Gly Ser Leu Glu Leu
625 630 635 640
Thr Met Asp Ser Asp Ser Ile Lys Asp Pro Asp Ile Thr Thr Leu Tyr
645 650 655
Asp Lys Asp Asn Tyr Ala Gly Thr Lys Lys Tyr Ile Phe Glu Asp Thr
660 665 670
Ala Arg Leu Ser Asp Phe Asn Asp Arg Thr Ser Ser Ile Lys Val Gly
675 680 685
Ser His Ile Ala Gly Val Arg Phe Tyr Glu His Tyr His Tyr Ser Gly
690 695 700
Asp Phe Ile Asp Leu Gly Ser Gly Glu Lys Leu Ser Leu Lys Asn His
705 710 715 720
Arg Leu Asn Lys Arg Leu Ser Ser Val Lys Phe Ala Asn Leu Gly Leu
725 730 735
Tyr Lys Glu Asp Asn Tyr Lys Gly Glu Lys Lys Leu Ile Phe Asp Asp
740 745 750
Ile Pro Asn Leu Thr Asp Phe Asn Asp Lys Thr Ser Ser Ile Val Val
755 760 765
Ser Pro Asn Val Ser Gly Thr Val Arg Leu Phe Glu His Ser Tyr Tyr
770 775 780
Lys Gly Glu Tyr Ile Glu Val Ser Gly Gly Gln Lys Leu Ser Leu Lys
785 790 795 800
Gly His Asn Leu Asn Arg Lys Ile Ser Ser Ile Lys Phe Leu Lys Glu
805 810 815
His Glu Val Ile Asn Gly Leu Tyr Glu Ile Ile Thr Ala Ile Asn Asn
820 825 830
Lys Ser Val Val Asp Trp Ser Lys Asp Lys Asn Ser Asn Val His Leu
835 840 845
Trp Glu Arg Arg Asn Gln Asp Asn Gln Lys Trp Tyr Phe Asp Tyr Asp
850 855 860
Ser Ser Lys Gln Ala Phe Gln Ile Lys Ser Lys Ala Asp Lys Asn Ile
865 870 875 880
Val Leu Ala Trp Asn Asp Phe Gly Gly Ser Lys Asn Val Phe Ala Thr
885 890 895
Tyr Asn Ser Lys Lys Asp Glu His Tyr Trp Ile Leu Asp Tyr Leu Gly
900 905 910
Glu Gly Asn Tyr Leu Ile Arg Asn Lys Lys Lys Gln Asn Leu Val Leu
915 920 925
Asp Val Asp Gly His Lys Thr Ala Asn Gly Thr Asn Ile Lys Val Asn
930 935 940
Glu Gln His Ala Leu Asp Asn Pro Ser Ile Glu Ala Gln Lys Phe Lys
945 950 955 960
Leu Asn Tyr Val Ile Lys
965
<210> 25
<211> 670
<212> БЕЛОК
<213> Bacillus sp.
<400> 25
Val Lys Asn Met Asn Ser Tyr Gln Asn Lys Asn Lys Tyr Glu Ile Leu
1 5 10 15
Asp Thr Ser Pro Asn Ser Ser Thr Met Ser Thr Arg Tyr Pro Arg Tyr
20 25 30
Pro Leu Ala Lys Asn Pro Gln Ile Ser Met Gln Asn Thr Asn Tyr Lys
35 40 45
Asp Trp Ile Asn Met Cys Ala Ser Arg Asn Leu Glu Asp Arg Ile Phe
50 55 60
Pro Thr Ser Ala Asn Asp Val Ile Thr Asn Ser Ile Asn Ile Ser Ser
65 70 75 80
Tyr Ile Ile Ser Met Leu Gly Met Pro Asn Leu Ser Ser Ile Ile Ala
85 90 95
Ile Trp Gly Ile Leu Phe Asn Ala Phe Trp Pro Val Ser Gly Asn Gln
100 105 110
Trp Asp Leu Tyr Met Asn His Val Gln Ala Leu Ile Lys Arg Glu Leu
115 120 125
Gln Asn Phe Ala Arg Glu Gln Ala Leu Arg Gln Leu Glu Gly Leu Gly
130 135 140
Gly Asn Leu Asp Leu Tyr Lys Glu Ala Leu Ala Glu Trp Glu Gln Asp
145 150 155 160
Arg Asp Asn Pro Thr Thr Lys Glu Arg Val Arg Asp Arg Phe Arg Ile
165 170 175
Leu Asp Gly Phe Phe Thr Gln Tyr Ile Pro Val Phe Arg Ile Gln Gly
180 185 190
Tyr Glu Val Gln Leu Leu Ser Val Tyr Val Lys Val Ala Asn Leu His
195 200 205
Leu Leu Leu Leu Arg Asp Ala Ser Met Phe Gly Ala Asp Trp Gly Met
210 215 220
Ser Gln Thr Asn Ile Asn Asp Asn Tyr Asn Arg Gln Met Lys Leu Thr
225 230 235 240
Ser Leu Tyr Thr Asn His Cys Val Asp Phe Tyr Asn Gln Gly Leu Asn
245 250 255
Glu Ala Arg Ala Leu Ser Asn Ser Asn Trp Asp Ile Phe Asn Asp Tyr
260 265 270
Arg Arg Glu Met Thr Leu Thr Val Leu Asp Ile Val Ala Leu Phe Pro
275 280 285
Ala Tyr Asp Tyr Arg Arg Tyr Pro Ile Thr Thr Lys Val Glu Leu Thr
290 295 300
Arg Glu Ile Tyr Thr Pro Ala Ile Ala Ser Gln Thr Trp Ser Asn His
305 310 315 320
Asn His Leu Ser Pro Asn Glu Asn Phe Gln Phe Tyr Glu Asn Asn Leu
325 330 335
Val Arg Pro Pro Ala Phe Phe Thr Trp Leu Asp Arg Thr Glu Met Phe
340 345 350
Ser Arg Tyr Leu Ser Thr Leu Val Ser Glu Ala Trp Gly Gly His Ile
355 360 365
Asn His Phe His His Thr Gly Glu Leu Pro Ser Ser Ser Arg Ser Gly
370 375 380
Phe Ile Gly Ser Asp Gln Arg Arg Val Ser Tyr Tyr Asp Phe Asn Phe
385 390 395 400
Val Gly Asn Asp Val Phe Arg Ile Tyr Ser Arg Val Met Ser Asn Gln
405 410 415
Ile Gly Asn Tyr Phe Gly Val Gly Gln Gly Asp Phe Phe Leu Val Asn
420 425 430
Arg Asp Asn Ser Asn Thr Lys Thr Ile Ser Phe Thr Thr Arg Ala Thr
435 440 445
Asn Ser Asn Gln Arg Ser Ile Phe Ser Gln Phe Pro Gly Glu Asn Ser
450 455 460
Asp Pro Pro Thr Ser Lys Asp Tyr Ser His Arg Leu Ser Trp Ile Ser
465 470 475 480
Gly Ala Phe Ile Gly Ser Asp Leu Ala Asn Val Leu Ser Tyr Gly Trp
485 490 495
Thr His Arg Ser Val Asp Pro Asn Asn Thr Ile Tyr Pro Asp Arg Ile
500 505 510
Thr Gln Val Pro Ala Val Lys Ala Ser Ser Ala Pro Asp Cys Thr Val
515 520 525
Ile Pro Gly Pro Gly Ser Thr Gly Gly His Leu Val Ser Phe Gly Arg
530 535 540
Ser Gly Arg Leu Asn Met Gln Phe Lys Phe Thr Asn Thr Gln Thr Glu
545 550 555 560
Tyr Arg Val Arg Ile Arg Tyr Ala Ser Thr Ala Ile Asn Thr Leu Tyr
565 570 575
Phe Ser Phe Ser Asp Tyr Asn Arg Asp Ile Ala Leu Asn Ser Thr Gly
580 585 590
Ala Leu Ser Leu Thr Asn Leu Arg Asn Glu Asn Phe Ala Tyr Phe Glu
595 600 605
Val Pro Gly Gly Ile Phe Arg Pro Ala Leu Gly Asn Thr Leu Met Ile
610 615 620
Ser Asn Trp Thr Thr Val Ala Pro Leu Leu Val Ile Asp Lys Ile Glu
625 630 635 640
Phe Ile Pro Ile Asn Ala Thr Thr Ala Arg Tyr Glu Arg Lys Gln Glu
645 650 655
Leu Glu Lys Ala Thr Lys Val Val Asn Ser Leu Phe Ile Asn
660 665 670
<210> 26
<211> 644
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 26
Val Lys Asn Met Asn Ser Tyr Gln Asn Lys Asn Lys Tyr Glu Ile Leu
1 5 10 15
Asp Thr Ser Pro Asn Ser Ser Thr Met Ser Thr Arg Tyr Pro Arg Tyr
20 25 30
Pro Leu Ala Lys Asn Pro Gln Ile Ser Met Gln Asn Thr Asn Tyr Lys
35 40 45
Asp Trp Ile Asn Met Cys Ala Ser Arg Asn Leu Glu Asp Arg Ile Phe
50 55 60
Pro Thr Ser Ala Asn Asp Val Ile Thr Asn Ser Ile Asn Ile Ser Ser
65 70 75 80
Tyr Ile Ile Ser Met Leu Gly Met Pro Asn Leu Ser Ser Ile Ile Ala
85 90 95
Ile Trp Gly Ile Leu Phe Asn Ala Phe Trp Pro Val Ser Gly Asn Gln
100 105 110
Trp Asp Leu Tyr Met Asn His Val Gln Ala Leu Ile Lys Arg Glu Leu
115 120 125
Gln Asn Phe Ala Arg Glu Gln Ala Leu Arg Gln Leu Glu Gly Leu Gly
130 135 140
Gly Asn Leu Asp Leu Tyr Lys Glu Ala Leu Ala Glu Trp Glu Gln Asp
145 150 155 160
Arg Asp Asn Pro Thr Thr Lys Glu Arg Val Arg Asp Arg Phe Arg Ile
165 170 175
Leu Asp Gly Phe Phe Thr Gln Tyr Ile Pro Val Phe Arg Ile Gln Gly
180 185 190
Tyr Glu Val Gln Leu Leu Ser Val Tyr Val Lys Val Ala Asn Leu His
195 200 205
Leu Leu Leu Leu Arg Asp Ala Ser Met Phe Gly Ala Asp Trp Gly Met
210 215 220
Ser Gln Thr Asn Ile Asn Asp Asn Tyr Asn Arg Gln Met Lys Leu Thr
225 230 235 240
Ser Leu Tyr Thr Asn His Cys Val Asp Phe Tyr Asn Gln Gly Leu Asn
245 250 255
Glu Ala Arg Ala Leu Ser Asn Ser Asn Trp Asp Ile Phe Asn Asp Tyr
260 265 270
Arg Arg Glu Met Thr Leu Thr Val Leu Asp Ile Val Ala Leu Phe Pro
275 280 285
Ala Tyr Asp Tyr Arg Arg Tyr Pro Ile Thr Thr Lys Val Glu Leu Thr
290 295 300
Arg Glu Ile Tyr Thr Pro Ala Ile Ala Ser Gln Thr Trp Ser Asn His
305 310 315 320
Asn His Leu Ser Pro Asn Glu Asn Phe Gln Phe Tyr Glu Asn Asn Leu
325 330 335
Val Arg Pro Pro Ala Phe Phe Thr Trp Leu Asp Arg Thr Glu Met Phe
340 345 350
Ser Arg Tyr Leu Ser Thr Leu Val Ser Glu Ala Trp Gly Gly His Ile
355 360 365
Asn His Phe His His Thr Gly Glu Leu Pro Ser Ser Ser Arg Ser Gly
370 375 380
Phe Ile Gly Ser Asp Gln Arg Arg Val Ser Tyr Tyr Asp Phe Asn Phe
385 390 395 400
Val Gly Asn Asp Val Phe Arg Ile Tyr Ser Arg Val Met Ser Asn Gln
405 410 415
Ile Gly Asn Tyr Phe Gly Val Gly Gln Gly Asp Phe Phe Leu Val Asn
420 425 430
Arg Asp Asn Ser Asn Thr Lys Thr Ile Ser Phe Thr Thr Arg Ala Thr
435 440 445
Asn Ser Asn Gln Arg Ser Ile Phe Ser Gln Phe Pro Gly Glu Asn Ser
450 455 460
Asp Pro Pro Thr Ser Lys Asp Tyr Ser His Arg Leu Ser Trp Ile Ser
465 470 475 480
Gly Ala Phe Ile Gly Ser Asp Leu Ala Asn Val Leu Ser Tyr Gly Trp
485 490 495
Thr His Arg Ser Val Asp Pro Asn Asn Thr Ile Tyr Pro Asp Arg Ile
500 505 510
Thr Gln Val Pro Ala Val Lys Ala Ser Ser Ala Pro Asp Cys Thr Val
515 520 525
Ile Pro Gly Pro Gly Ser Thr Gly Gly His Leu Val Ser Phe Gly Arg
530 535 540
Ser Gly Arg Leu Asn Met Gln Phe Lys Phe Thr Asn Thr Gln Thr Glu
545 550 555 560
Tyr Arg Val Arg Ile Arg Tyr Ala Ser Thr Ala Ile Asn Thr Leu Tyr
565 570 575
Phe Ser Phe Ser Asp Tyr Asn Arg Asp Ile Ala Leu Asn Ser Thr Gly
580 585 590
Ala Leu Ser Leu Thr Asn Leu Arg Asn Glu Asn Phe Ala Tyr Phe Glu
595 600 605
Val Pro Gly Gly Ile Phe Arg Pro Ala Leu Gly Asn Thr Leu Met Ile
610 615 620
Ser Asn Trp Thr Thr Val Ala Pro Leu Leu Val Ile Asp Lys Ile Glu
625 630 635 640
Phe Ile Pro Ile
<210> 27
<211> 572
<212> БЕЛОК
<213> Streptomyces sp.
<400> 27
Val Arg Thr Leu Leu Ala Gln Leu Leu Glu Pro Gly Gly Arg Val Gly
1 5 10 15
Leu Glu Leu Gly Gln Leu Gly Ala Arg Asp Asp Thr Leu Leu Glu Leu
20 25 30
Cys Gly Gly Gly Leu Glu Val His Thr Gln Leu Leu Arg Arg Gly Arg
35 40 45
Gly Leu His Gly Leu Gly Val Leu Val Arg Arg His Gly Val Lys Gly
50 55 60
Ile Glu His Ala Gly Glu Leu Arg Pro Cys Arg Leu Pro Val Thr Trp
65 70 75 80
Arg Gly Asp Arg His Gly Arg Phe Pro Arg Gly Tyr Pro His Phe Pro
85 90 95
Leu Leu Leu Pro Ala Arg Leu Arg Ala Gly Gly Asp Pro Arg Glu Val
100 105 110
Gly Ala Asp Leu Glu Arg Ala Pro Ala Arg Arg Ala Pro Asp Ala Ala
115 120 125
Arg Arg Asp Thr Gly Arg Gly Leu Arg Ser Ala Pro Gly Gly Arg Arg
130 135 140
Gly Arg Gly Ser Arg Ala Ala Ala Arg Arg Pro Gly Arg Pro Gln Arg
145 150 155 160
Asp Pro Ala Leu His Gly Asp Asp Gly Arg Arg His Pro Gln Gly Pro
165 170 175
Pro Val Arg Gly Gly Gly Gly Gly Val Gly Arg Gly Pro Arg Arg Gly
180 185 190
Ala Arg Pro Ala Pro Ala Arg Arg His Pro Arg Leu Gly Ala Ala Ala
195 200 205
Gly Pro Pro Gly Asp Gly Ala Pro Arg Asp Asp Gly Arg Arg Asp Arg
210 215 220
Ala Gly Arg Arg Gly Arg Arg Arg Pro Arg Arg Pro Ala Val Ala Gly
225 230 235 240
Pro Ser Ala Pro Pro Gln Gly Pro His Leu Pro Asp Pro Arg Arg Arg
245 250 255
Pro Ala Val Ala Gly Gly Pro Val Val Ala Gly Gly Gly Arg Gly Ala
260 265 270
Arg Ser Gly Gly Gly Val His Arg His Arg Ala Arg Pro His Arg Glu
275 280 285
Gln His Pro Gly Pro Pro Arg Arg Gly Glu Glu Gly Pro Glu Ala Ala
290 295 300
Gln Gly Ala Ala Ala Arg Glu Ala Gly Ala Leu Arg Glu Ala Gly His
305 310 315 320
Arg Leu Pro His Arg Arg Pro Ser Leu Gly Val Pro Gln Gly Arg Arg
325 330 335
Gly Leu Arg Pro Glu Arg Glu Ala Ala Pro Arg Pro Gly Leu Asn Arg
340 345 350
Pro His Gly Gly Arg Pro Val Pro Gly Pro Gly Arg Leu Gly Ile Leu
355 360 365
Thr Pro Pro Asp His Arg Asp His Ala Gly Gly Ala Leu Ala Ala Ser
370 375 380
Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu Ala Gln Ala Arg
385 390 395 400
Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro Ala Thr Val Asn
405 410 415
Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ser Ala Val Pro Asp Ser
420 425 430
Asp Val Val Arg Met Val Arg Gly Trp Gly Leu Gln Glu His Ala Pro
435 440 445
Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val Arg Gln Ala Leu
450 455 460
Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr Val Glu Arg Glu
465 470 475 480
Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu Gly Gln Pro Gly
485 490 495
Leu Ser Phe Tyr Glu Glu Ser Gly Asp Arg Thr Arg Phe Tyr Arg Asp
500 505 510
Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp His Met Tyr Ala
515 520 525
Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro Lys Ala Glu Val
530 535 540
Leu Ala Leu Asp Leu Thr Asp Ile Val Pro Phe Thr Val Arg Leu Asn
545 550 555 560
Ala Ile Val Val Arg Gln Ala Leu Ser Val Ser Val
565 570
<210> 28
<211> 434
<212> БЕЛОК
<213> Bacillus sp.
<400> 28
Leu Val Leu Ser Phe Tyr Ile Ala Lys Val Ile Leu Phe Arg Asn Lys
1 5 10 15
Ser Ile Ser Asn Val Gln Tyr Lys Ile Ile Phe Cys Asn Leu Val Gly
20 25 30
Ile Leu Pro Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys
35 40 45
Lys Val Met Ala Gly Leu Gly Val Gly Ala Met Ser Leu Ser Ile Trp
50 55 60
Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala Asn Asn Glu Tyr His Thr
65 70 75 80
Ile His Leu Ala Ala Asn Ser Asn Asn Val Trp Asp Val Tyr Lys Gly
85 90 95
Trp Asp Ser Asp Asn Ala Gly Ile Val Leu Tyr Asn Gly Ser Arg Gly
100 105 110
Asp Asn Glu Gln Phe Ala Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala
115 120 125
Ile Val Asn Lys Asn Ser Gly Lys Pro Val Gly Leu Gly Asn Ala Tyr
130 135 140
Ile Ser Thr Asp Gly Val Arg Asp Gly Phe Ser Phe Asn Asp Gln Gly
145 150 155 160
Leu Arg Gln Thr Asn Trp Thr Gly Ala Pro Asn Glu Gln Trp Tyr Leu
165 170 175
Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly Thr Gly
180 185 190
Lys Val Ala Ser Trp Ala Trp Asn Gly Ile Ser Gly Pro His Ser Ala
195 200 205
Glu Tyr Val Asp Leu Asp Glu Ser Asn Pro Ser Asp Asn Asn Arg Leu
210 215 220
Phe His Ile Pro Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu Pro
225 230 235 240
Ala Val Gly Thr Arg Pro Asn Ala Pro Asp Tyr Asn Thr Ser Gly Ala
245 250 255
Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala
260 265 270
Ser Leu Ile Pro Ser Ile Met Val Lys Asp Asn Gly Ala Ser Asp Tyr
275 280 285
Thr Lys Ile His Asn Ser Pro Tyr Tyr Ala Leu Val Lys Glu Glu Tyr
290 295 300
Trp Glu Lys Val Arg Ser Glu Ile Ile Pro Ala Gly Ala Thr Ser Lys
305 310 315 320
Tyr Thr Val Lys Ser Gly Val Ser Glu Ile Asp Gln Lys Lys Met Ser
325 330 335
Asp Thr Leu Ala Met Ser Phe Gly Ser Asp Leu Gly Leu Lys Phe Gly
340 345 350
Glu Ser Ser Leu Ser Ile Lys Ser Ser Val Ser Arg Thr Ile Gln Thr
355 360 365
Glu Val Thr Lys Thr Ser Thr Asn Ala Thr Glu Glu Thr Glu Glu Lys
370 375 380
Ser Ile Pro Ser Ile Pro Gly Lys Asn Thr Gly Phe Thr Ala Tyr Gln
385 390 395 400
Leu Val Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly Ser Val Val Thr
405 410 415
Asp Pro Ser Ile Val Arg Asp Ser Lys Gln Thr Ile Ala Arg Thr Ile
420 425 430
Ala Asn
<210> 29
<211> 272
<212> БЕЛОК
<213> Bacillus sp.
<400> 29
Met Tyr Lys Cys Tyr Phe Ile Trp Gly Lys Leu His Pro Asp Tyr Gly
1 5 10 15
Gly Ala Leu Phe Ser Ile Phe Val Gln Lys Leu His Glu Lys Glu Arg
20 25 30
Val Tyr Met Asn Tyr Pro Ile Leu Asn Asn Thr Asn Ile Asn Ser Val
35 40 45
Ile Gln Pro Pro Arg Ile His Arg Pro Gly Arg Ile Ile Thr Leu Thr
50 55 60
Val Pro Ser Arg Asn Glu Ser Asn Met Lys Asn Ile Phe Gln Leu Glu
65 70 75 80
Pro Gln Tyr Ile Ala Gln Ala Leu Thr Ala Ala Gln Ala Phe Gln Gly
85 90 95
Ala Ile Gly Ser Asn Leu Asn Phe Asp Phe Asp Lys Ala Ala Gln Ile
100 105 110
Ala His Asn Leu Pro Asn Arg Tyr Ser Asn Val Ile Gly Leu Glu Arg
115 120 125
Pro Val Ile Gln Gln Thr Leu Gln Val Ser Pro Met Ile Ser Lys Leu
130 135 140
Lys Glu Thr Leu Gly Asn Phe Leu His Phe Ile Asp Ile Ser Asn Ala
145 150 155 160
Gly Phe Trp Asn Glu Val Glu Ala Ala Ile Thr Tyr Thr Phe Thr Asn
165 170 175
Leu Tyr Thr Gln Ala Asn Ser Ala Trp Ile Phe Trp Ser Asn Ser Thr
180 185 190
Ser Thr Ser Thr Ser Tyr Tyr Tyr Asn Ile Leu Phe Ala Ile Arg Asp
195 200 205
Ser Ser Asn Arg Lys Met Ile Val Ile Pro Ile Ala Phe Asp Ile Thr
210 215 220
Val Arg Leu Glu Lys Gln Lys Val Leu Trp Ile Thr Ile Lys Asp Trp
225 230 235 240
Ala Glu Tyr Arg Val Thr Ile Lys Cys Ile Cys Val His Leu Phe Leu
245 250 255
Gln Gly Thr Pro Asn Gln Ser Ile Val Asp Val Phe Asn Pro Lys Ser
260 265 270
<210> 30
<211> 549
<212> БЕЛОК
<213> Bacillus sp.
<400> 30
Met Leu Gln Asn Asn Leu Glu Thr Lys Val Leu Pro Ser Trp Val Asp
1 5 10 15
Asp Phe Asn Gly Ile Phe Gly Leu Phe Asp Gly Ile Ser Ser Ile Phe
20 25 30
Asp Lys Ile Phe Gly Thr Asn Ile Gly Asp Glu Asn Ile Glu Leu Glu
35 40 45
Glu Ile Leu Lys Asn Gln Glu Leu Leu Asn Glu Leu Arg Asp Lys Leu
50 55 60
Ser Gly Ile Ser Gly Asp Leu Glu Thr Ile Ile Lys Asn Gly Glu Leu
65 70 75 80
Asn Thr Glu Leu Leu Gln Lys Leu Ile Ser Ile Ser Asn Gly His Gly
85 90 95
Asp Met Leu Asn Asn Ile Gln Ser Gln Ile Asp Ser Leu Arg Thr Phe
100 105 110
Ile Ala Gly Tyr Leu Ala Glu Ile Ser Gly Met Leu Arg Lys Ile Thr
115 120 125
Gln Gln Asn Thr Phe Ile Ser Thr Gln Leu Met Tyr Ile Thr Gln Gln
130 135 140
Leu Leu Val Ile Ser Gln Lys Leu Asp Thr Leu Asn Val Asn Val Leu
145 150 155 160
Ile Ser Thr Thr Ile Thr Glu Ile Thr Pro Ser Tyr Gln Arg Met Lys
165 170 175
Tyr Ala Asn Lys Lys Phe Asn Glu Leu Ser Thr Ala His Thr Glu Lys
180 185 190
Tyr Leu Arg Thr Ile Lys Asp Asn Ser Asn Ile Val Gln Ile Asp Thr
195 200 205
Asn Asp Leu Gln Lys Met Asn Glu Leu Ile Glu Leu Ala Lys Asn Ile
210 215 220
Thr Ala Asn Ser His Asp Asn Phe Glu Phe Tyr Leu Glu Thr Phe Gln
225 230 235 240
Asp Val Met Val Gly Glu Thr Leu Phe Glu Arg Ser Ala Leu His Thr
245 250 255
Ile Ser Glu Leu Ile Arg Ser Asp Gln Thr Pro Lys Glu Tyr Gly Ser
260 265 270
Glu Val Ala Lys Ala Tyr Thr Tyr Leu Ile Asn Leu Ile Ser Val Gln
275 280 285
Ala Gln Ser Tyr Ala Thr Leu Ala Ala Cys Arg Lys Val Leu Gly Leu
290 295 300
Ser Asp Ile Asp Tyr Lys Pro Ile Leu Leu Gln Tyr Ile Lys Lys Gln
305 310 315 320
Leu Asn Ile Phe Lys Ser Glu Glu Leu Pro Lys Leu Ser Asn Glu Phe
325 330 335
Thr Ala Phe Asn Tyr Ile Gly Ile Glu Gly Glu Asn Thr Pro Arg His
340 345 350
Cys Val Val Lys Ala Asp Pro Gly Tyr Ala Val Val Gly Leu Glu Ile
355 360 365
Ile Lys Ser Asp Asn Asp Leu Asn Leu Ile Ala Tyr Gln Ala Lys Leu
370 375 380
Lys Gln Asn Tyr Glu Leu Asp Lys Glu Thr Ile Ser Lys Ile Thr Val
385 390 395 400
His Lys Ile Asn Glu Val Phe Gly Gln Ile Glu Asp Thr Phe Ala Ile
405 410 415
Gly Ile Phe Cys Ala Lys Leu Glu Phe Pro Gln Gly Tyr Val Ile Thr
420 425 430
Gln Leu Asp Phe Ser Asn Asp Lys Gly Tyr Phe Val Thr Tyr Glu Leu
435 440 445
Thr Ala Asn Arg Tyr Asn Pro Ser Thr Gly Asp Leu Asp Leu Ser Asp
450 455 460
Ser Ile Thr Lys Asn Ser Ser Thr Glu Glu Asn Asn Lys Glu Thr Ile
465 470 475 480
Ile Glu Leu Glu Pro Glu Thr Lys Met Phe Phe Pro Ser Lys Ile Ser
485 490 495
Asp Thr Phe Leu Thr Pro Met Tyr Ser Phe Asn Ile Asp Ile Val Pro
500 505 510
His Ser Thr Tyr Tyr Arg Val Thr Cys Glu Asn Tyr Leu Pro Gln Tyr
515 520 525
Leu Glu Ala Ser Asp Leu Ser Asn Lys Glu Thr Ser Leu Leu Tyr Ile
530 535 540
Val Asp Asn Thr Ile
545
<210> 31
<211> 404
<212> БЕЛОК
<213> Bacillus sp.
<400> 31
Met Lys Ser Ile Ser Lys Lys Ile Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ala Ser Gln Ala Ala Ala Pro Glu
20 25 30
Lys Asn Arg Tyr Tyr Ser Ile His Leu Thr Asn Asn Ile Glu Lys Val
35 40 45
Trp Asp Val Tyr Lys Gly Trp Thr Ser Asn Asp Ala Gly Leu Leu Leu
50 55 60
Tyr Lys Ala Asn Gly Gln Asp Asn Gln Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Leu Gly Gly Gly Tyr Asn Tyr Ser Asp Gly Val Arg Asp Met Ser
100 105 110
Asn Tyr Ser Gly Asn Pro Ala Glu Ile Leu Gly Gln Asn Asp Trp Thr
115 120 125
Gly Ala Asp Thr Asp Lys Trp Tyr Leu Arg Asp Lys Gly Tyr Gly Asn
130 135 140
Tyr Glu Val Val Asn Gln Gly His Gly Lys Val Ala Ser Tyr Ala Ala
145 150 155 160
Thr Asn Ile Ala Pro Tyr Leu His Tyr Val Asp Leu Asp Asp Ser Asn
165 170 175
Pro Ser Asp Gly Asp Arg Leu Phe Lys Val Ser Lys Ser Pro Trp Val
180 185 190
Tyr Gly Asp Ile Pro Gly Leu Asn Trp Asp Tyr Gly Thr Phe Ser Leu
195 200 205
Pro Gln Leu Pro Ala Thr Gly Asn Arg Pro Asp Ala Pro Asn Tyr Thr
210 215 220
Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val
225 230 235 240
Gly Ala Ser Leu Ile Pro Cys Ile Met Val Lys Asp Ser Gln Ala Ser
245 250 255
Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Ile Lys Glu
260 265 270
Glu Tyr Trp Asp Lys Thr Phe Ser Ser Val Ile Pro Ser Gly Tyr Thr
275 280 285
Lys Asn Tyr Ser Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln Arg
290 295 300
Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys
305 310 315 320
Phe Gly Asp Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr Ile
325 330 335
Gln Thr Glu Ile Thr Ser Thr Asn Thr Glu Ala Ser Glu Glu Thr Val
340 345 350
Thr Ser Thr Val Thr Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr Glu
355 360 365
Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Thr Ile
370 375 380
Val Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile Ala Arg
385 390 395 400
Lys Asn Ser Gln
<210> 32
<211> 192
<212> БЕЛОК
<213> Streptomyces sp.
<400> 32
Leu Ala Ala Ser Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu
1 5 10 15
Ala Gln Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Phe Asp Phe Glu Ala Ile Arg Glu Ala Ala Ala Ala
35 40 45
Val Pro Asp Ser Thr Val Val Arg Met Val Arg Gly Trp Gly Leu Gln
50 55 60
Glu Tyr Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr
85 90 95
Val Glu Arg Glu Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu
100 105 110
Gly Arg Pro Gly Leu Ser Phe Tyr Glu Glu Ser Ala Glu Arg Thr Arg
115 120 125
Phe Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Glu
130 135 140
His Met Tyr Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro
145 150 155 160
Lys Ala Glu Thr Val Ala Leu Glu Leu Thr Asp Val Val Pro Phe Ala
165 170 175
Val Arg Leu Asn Ala Ile Val Val Arg Gln Pro Leu Gly Leu Ala Ala
180 185 190
<210> 33
<211> 303
<212> БЕЛОК
<213> Bacillus sp.
<400> 33
Met Glu Arg Arg Leu Arg Ile Met Ala Ile Ala Asp Leu Asp Val Tyr
1 5 10 15
Leu Ile Ala Leu Ile Lys Lys Thr Tyr Pro Glu Tyr Ala Ser Tyr Glu
20 25 30
Ala Thr Ser Asp Thr Ser Lys Leu Ser Ile Lys Asn Thr Glu Ser Tyr
35 40 45
Gly Phe Glu Ile Lys Glu Asn Lys Pro Gln Gly Thr Met Phe Met Gly
50 55 60
Glu Ser Val Leu Lys Asn Asp Thr Asn Glu Thr Gln Thr Ile His Ser
65 70 75 80
Asp Ala Phe Thr Lys Thr Ile Thr Asp Ser Val Thr Leu Ser Val Thr
85 90 95
Asn Gly Ile Thr Ala Gly Val Ser Ile Ser Ile Ser Gly Lys Ile Phe
100 105 110
Gly Ile Gly Ala Glu Ser Ser Met Ser Phe Glu Val Ser Thr Ser Thr
115 120 125
Thr Asn Glu Gln Thr Ser Glu Glu Ser Val Ala Tyr Thr Val Pro Ser
130 135 140
Gln Thr Val Val Val Pro Ala Lys Thr Thr Tyr Tyr Val Tyr Thr Ser
145 150 155 160
Leu Gln Arg Asn Gln Leu Glu Gly Thr Val Arg Leu Lys Ala Asp Leu
165 170 175
Ser Glu Arg Phe Ser Val Arg Leu Lys Tyr Gly Ser Gly Gln Ala Glu
180 185 190
Ile Gly Gly Asp Leu Tyr Tyr Phe Ile Asn Glu Phe His Ser Ala Leu
195 200 205
Pro Leu Pro Ser Gly Ile Ser Leu Asn His Lys Asp Lys Ser Ile His
210 215 220
Phe Glu Gly Val Ala Glu Tyr Ile Tyr Gly Thr Gly Thr Lys Phe Tyr
225 230 235 240
Val Thr Ile Ser Asp Thr Pro Ser Ser Gln Gly Ile Gln Glu His Lys
245 250 255
Ser Phe Asp Ala Lys Thr Gly Leu Gly Thr Tyr Glu Leu Arg Leu Asn
260 265 270
Gly Lys Asn Phe Asp Phe Asp Leu Asn Asp Leu Lys Glu Tyr Met Asp
275 280 285
Ser Lys Glu Phe Glu Lys Leu Lys Glu Gln Glu Asn Glu Ile Val
290 295 300
<210> 34
<211> 383
<212> БЕЛОК
<213> Bacillus sp.
<400> 34
Met Lys Lys Lys Glu Ile Lys Arg Phe Pro Leu Pro Lys Lys Val Ile
1 5 10 15
Thr Gly Leu Phe Ala Gly Ala Met Ala Leu Ser Ile Trp Ala Pro Glu
20 25 30
Ser Gln Ala Ala Thr Pro Glu Lys Asn Lys Tyr Tyr Asn Ile Asn Leu
35 40 45
Lys Ala Asn Thr Trp Leu Lys Trp Asn Val Glu Gly Ala Ser Thr Asn
50 55 60
Asn Gly Pro Thr Ile Arg Leu Trp Gly Gly Ser Asn Asn Glu Asn Glu
65 70 75 80
Lys Phe Thr Phe Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile Val Asn
85 90 95
Lys Asn Ser Gly Lys Pro Val Thr Val Pro Ser Gly Ser Ser Ile Leu
100 105 110
Ser Gln Asn Asn Trp Thr Gly Ser Ser Asn Glu Gln Trp Tyr Leu Arg
115 120 125
Asn Gln Gly Asn Asn Tyr Tyr Glu Ile Val His Gln Gly Thr Gly Lys
130 135 140
Val Ala Ser Tyr Ala Arg Asn Gly Asn Ala Glu Tyr Val Asp Leu Asp
145 150 155 160
Glu Ser Asn Pro Tyr Asp Pro Asp Arg Val Phe Gln Ile Ser Asp Ala
165 170 175
Gly Ala Ser Val Gln Leu Pro Thr Leu Pro Thr Ile Gly Ser Arg Pro
180 185 190
Asn Ala Pro Glu Tyr Asn Pro Thr Gly Pro Ile Asp Gln Gln Leu Pro
195 200 205
Gln Asn Ser Gly Thr Val Val Val Gly Ala Thr Ser Ile Pro Cys Ile
210 215 220
Met Val Asn Asp Asn Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser
225 230 235 240
Pro Tyr Tyr Val Leu Val Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser
245 250 255
Glu Ile Ile Pro Ala Gly Gly Ser Ser Lys Tyr Thr Val Lys Thr Gly
260 265 270
Val Ser Thr Glu Asp Gln Gln Arg Met Thr Asp Thr Leu Ser Met Ser
275 280 285
Phe Gly Ala Asp Leu Gly Phe Lys Phe Lys Ala Leu Ser Ala Ser Leu
290 295 300
Lys Tyr Gly Ile Ser Lys Thr Leu Gln Thr Glu Ile Ser Thr Thr Ser
305 310 315 320
Thr Glu Ser Thr Glu Ser Thr Glu Ser Thr Glu Glu Lys Thr Ile Thr
325 330 335
Ser Leu Pro Gly Lys Asn Thr Gly Phe Thr Val Tyr Gln Leu Val Thr
340 345 350
Lys Tyr Thr Leu Lys Arg Thr Asp Asn Ser Val Val Ser Ser Ser Trp
355 360 365
Thr Val Arg Asp Pro Asn Gln Thr Leu Val Arg Thr Ile Pro Asn
370 375 380
<210> 35
<211> 556
<212> БЕЛОК
<213> Bacillus sp.
<400> 35
Leu Cys Tyr Met Asn Lys Gln Asn Thr Gln Val Ala Glu Leu Pro Val
1 5 10 15
Ile Asp Glu Phe Asn Gly Ile Ile Gly Phe Ala Gln Asn Ile Gly Gly
20 25 30
Ile Met Asn Met Ile Ala Gly Thr Pro Ser Val Asp Asp Met Thr Leu
35 40 45
Asp Gln Leu Val Thr Asn Tyr Asp Ala Leu Val Asp Ile Gln Thr Ser
50 55 60
Ile Ala Gly Ile Asn Lys Glu Leu Thr Asp Val Ile Ala Asn Gly Ser
65 70 75 80
Glu Asp Thr Glu Leu Ile Asn Gln Leu Ile Asn Ile Asn Thr Ala Gln
85 90 95
Ser Ala Glu Leu Asp Glu Ile Asn Gly Glu Ile Gln Asp Ile Asp Asn
100 105 110
Met Leu Asn Ala Tyr Leu Pro Glu Ile Ile Asn Met Leu Glu Gly Ile
115 120 125
Ser Gln Gln Leu Val Gly Ile Ser Gln Gln Ile Asn Gln Met Met Lys
130 135 140
Met Leu Asn Ala Ile Ser Lys Gln Leu Asn Lys Ile Ala Ala Asn Val
145 150 155 160
Ala Leu Asn Ser Thr Ile Ala Glu Ile Glu Pro His Tyr Gln Lys Ile
165 170 175
Lys Tyr Val Ile Thr Gln Phe Asn Glu Leu Asn Asp Glu Leu Val Thr
180 185 190
Ala Tyr Ser Ser Gln Ile Asn Asn Asn Lys Leu His Leu Gly Thr Asp
195 200 205
Asp Ile Asp Thr Gly Ala Leu Ala Ala Val Ile Ala Trp Ala Lys Met
210 215 220
Val Val Ala Ile Asp Leu Asn Asp Leu Arg Leu Ser Leu Asp Ala Met
225 230 235 240
Asn Asp Ala Ile Leu Gly Asp Asn Leu Tyr Gly Asn Ser Ala Leu Val
245 250 255
Ser Thr Ala Asp Phe Tyr Thr Ala Asn Thr Thr Tyr Ala Gln Ser Ile
260 265 270
Tyr Thr Ser Met Tyr Ser Tyr Leu Ser Thr Leu Cys Gly Leu Gln Thr
275 280 285
Asn Ala Tyr Met Leu Val Asp Thr Ala Arg Lys Ile Leu Ser Leu Pro
290 295 300
Glu Glu Asp Asn Gln Asp Leu Met Asn Ser Arg Ile Ser Gln Gln Lys
305 310 315 320
Glu Leu Phe Thr Ser Leu Ser Ser Gly Phe Ser Asn Val Phe Tyr Ser
325 330 335
Thr Glu Ser Ile Asp Met Asp Pro Gly Ala Ser Ala Pro Met Asn Gln
340 345 350
Ser Tyr Glu Ile Asp Leu Val Pro Pro Ala Gly Asn Ala Leu Leu Gly
355 360 365
Phe Asn Met Thr Ser Glu Pro Asp Ser Asn Gly Asp Val Gln Asn Gly
370 375 380
Lys Leu His Val Glu Tyr Tyr Tyr Gly Lys Leu Leu Thr Asn Tyr Gly
385 390 395 400
Val Asp Val Ala Ser Ile Asn Val Ala Thr Ala Glu Phe Ser Met Ala
405 410 415
Asn Tyr Leu Pro Ser Ser Gly Ile Tyr Asn Val Pro Asp Gly Gly Leu
420 425 430
Pro Thr Ile Asp Ser Asn Thr Ile Val Val Gly Phe Lys Leu Lys Ile
435 440 445
Thr Thr Asp Asn Met Gly Gln Ala Phe Met Val Pro Ser Val Val Thr
450 455 460
Ala Pro Tyr Asp Ser Thr Lys Asn Gly Val Gly Thr Thr Thr Lys Asp
465 470 475 480
Val Val Ile Pro Gln Ser Asp Asn Asn Ser Ile Thr Asn Gln Pro Met
485 490 495
Gly Ile Val Ser Asp Gln Phe Leu Ser Pro Ile Leu Asn Met Thr Met
500 505 510
Gly Lys Asp Glu Asn Gly Gln Tyr Cys Phe Met Val Glu Ser Ala Leu
515 520 525
Lys Asn Tyr Val Asn Ala Thr Asp Met Lys Asp Ala Asp Thr Asp Leu
530 535 540
Ala Val Ser Pro Tyr Tyr Ser Thr Asp Thr Ala Glu
545 550 555
<210> 36
<211> 684
<212> БЕЛОК
<213> Bacillus sp.
<400> 36
Met Asn Ser Asn Asp Lys Ser Glu Lys Asn Val Ile Asp Ala Ser Ser
1 5 10 15
Asp Gln Ala Val Ser Thr Asn Ser Val Arg Tyr Pro Leu Ala Ser Asp
20 25 30
Gln Ile Asn Thr Leu Gln Asn Ile Asn Tyr Lys Asp Tyr Leu Arg Met
35 40 45
Ser Glu Glu Gln Asn Val Asp Phe Leu Thr Asn Thr Glu Val Phe Ala
50 55 60
Ser Thr Gln Asp Ser Ile Lys Leu Gly Met Asn Ile Val Gly Gln Ile
65 70 75 80
Leu Thr Ser Leu Gly Ile Pro Phe Val Lys Pro Ile Ile Ser Phe Tyr
85 90 95
Thr Val Ile Ile Gly Leu Leu Trp Pro Ser Ser Gly Lys Asn Pro Trp
100 105 110
Lys Phe Phe Met Glu Gln Val Glu Glu Leu Ile Asp Gln Lys Ile Glu
115 120 125
Glu Tyr Ala Arg Asn Lys Ala Leu Ala Glu Leu Glu Gly Leu Gly Asn
130 135 140
Asn Phe Glu Leu Tyr Arg Val Ala Leu Glu Glu Trp Lys Glu Asn Pro
145 150 155 160
Asn Ile Ser Arg Thr Thr Arg Asp Val Arg Thr Arg Phe Glu Ile Leu
165 170 175
Asp Gly Leu Phe Thr Gln Tyr Met Pro Ser Phe Lys Val Ser Gly His
180 185 190
Glu Val Pro Leu Leu Thr Val Tyr Ala Gln Ala Ala Ser Leu His Leu
195 200 205
Leu Leu Leu Lys Asp Ala Ser Ile Phe Gly Glu Glu Trp Gly Leu Pro
210 215 220
Thr Ala Thr Ile Asn Asn Tyr Tyr Lys Arg Gln Met Asn Leu Thr Ala
225 230 235 240
Gln Tyr Ser Asp His Cys Val Asn Trp Tyr Asn Thr Gly Leu Asp Arg
245 250 255
Leu Lys Gly Ser Asn Ala Lys Ser Trp Leu Ser Tyr His Arg Phe Arg
260 265 270
Arg Glu Met Thr Leu Met Val Leu Asp Leu Val Ala Leu Tyr Pro Asn
275 280 285
Tyr Asp Val Arg Thr Tyr Pro Ile Glu Thr Thr Val Gln Leu Ser Arg
290 295 300
Glu Val Tyr Thr Asp Pro Ile Gly Tyr Asn Pro Lys Asn Pro Glu Asn
305 310 315 320
Ser Gly Phe Gly Leu Pro Trp Ala Ser Leu Asn Ser His Asn Phe Thr
325 330 335
Gln Ile Glu Arg Glu Val Ile Arg Pro Pro His Ala Phe Asn Ile Leu
340 345 350
Asn Ser Val Glu Ile Gly Thr Ile Lys Gln Lys Leu Leu Phe Asn Asp
355 360 365
Lys Glu Tyr Ile Asn Tyr Trp Ser Gly His Leu Leu Glu Tyr Thr Tyr
370 375 380
Ala Asn Asp Ser Glu Ile Tyr Lys Lys Asp Tyr Gly Asn Leu Asn Thr
385 390 395 400
Phe Glu Lys Asn Lys Ile Asp Leu Lys Asp Lys Asp Ile Tyr Arg Ile
405 410 415
Asp Ser Thr Ala Val Cys Leu Ala Asn Phe Tyr Thr Tyr Ile Tyr Gly
420 425 430
Ser Thr Lys Ser Asn Phe His Met Val Asn Thr Asp Asn Leu Lys Pro
435 440 445
Val Ser Tyr Ser Tyr Ser Lys Thr His Thr Ala Ser Pro Ser Lys Ile
450 455 460
Gln Asn Tyr Asp Ser Glu Asn Ala Leu Pro Gln Glu Thr Thr Asp Glu
465 470 475 480
Pro Ser Tyr Gln Ser Tyr Asn His Arg Leu Ala His Ile Thr Thr Tyr
485 490 495
Pro Phe Trp His Thr Asn Gly Ser Asn Ser Gln Ser Gly Phe Leu Pro
500 505 510
Val Phe Val Trp Thr His Arg Ser Val Asp Leu Tyr Asn Phe Ile Asp
515 520 525
Ser Lys Lys Ile Thr Gln Ile Pro Ala Val Lys Gly Leu Gln Asn Tyr
530 535 540
Ile Gln Lys Gly Ala Asn Ile Glu Glu Gly Ser Gly Tyr Thr Gly Gly
545 550 555 560
Asp Val Val Thr Leu His Asn Phe Glu Ser Ser Pro Lys Leu Val His
565 570 575
Leu Tyr Ala Thr Ala Ala Glu Asn Ala Leu Ser Gln Lys Tyr Arg Val
580 585 590
Arg Val Arg Tyr Val Ser Glu Gly Asn Gly Glu Leu Tyr Met Arg Val
595 600 605
Lys Lys Ser Pro Asp Glu Phe Phe Gly Gln Thr Ala Ser Ala Lys Ile
610 615 620
Thr Gln Thr Lys Asn Ser Glu Glu Lys Leu Thr Ser Lys Asn Phe Lys
625 630 635 640
Tyr Thr Glu Phe Asp Glu Leu Val Ser Pro Leu Val Pro Lys Phe Ile
645 650 655
Ile Glu Ile Thr Ala Val Asn Met Leu Phe Cys Pro His Cys Lys Leu
660 665 670
Tyr Val Asp Lys Ile Glu Phe Ile Pro Val Ala Glu
675 680
<210> 37
<211> 682
<212> БЕЛОК
<213> Bacillus sp.
<400> 37
Met Asn Ser Asn Asp Lys Ser Glu Lys Asn Val Ile Asp Ala Ser Ser
1 5 10 15
Asp Gln Ala Val Ser Thr Asn Ser Val Arg Tyr Pro Leu Ala Ser Asp
20 25 30
Gln Ile Asn Thr Leu Gln Asn Ile Asn Tyr Lys Asp Tyr Leu Arg Met
35 40 45
Ser Glu Glu Gln Asn Val Asp Phe Leu Thr Asn Thr Glu Val Phe Ala
50 55 60
Ser Thr Gln Asp Ser Ile Lys Leu Gly Met Asn Ile Val Gly Gln Ile
65 70 75 80
Leu Thr Ser Leu Gly Ile Pro Phe Val Lys Pro Ile Ile Ser Phe Tyr
85 90 95
Thr Val Ile Ile Gly Leu Leu Trp Pro Ser Ser Gly Lys Asn Pro Trp
100 105 110
Lys Phe Phe Met Glu Gln Val Glu Glu Leu Ile Asp Gln Lys Ile Glu
115 120 125
Glu Tyr Ala Arg Asn Lys Ala Leu Ala Glu Leu Glu Gly Leu Gly Asn
130 135 140
Asn Phe Glu Leu Tyr Arg Val Ala Leu Glu Glu Trp Lys Glu Asn Pro
145 150 155 160
Asn Ile Ser Arg Thr Thr Arg Asp Val Arg Thr Arg Phe Glu Ile Leu
165 170 175
Asp Gly Leu Phe Thr Gln Tyr Met Pro Ser Phe Lys Val Ser Gly His
180 185 190
Glu Val Pro Leu Leu Thr Val Tyr Ala Gln Ala Ala Ser Leu His Leu
195 200 205
Leu Leu Leu Lys Asp Ala Ser Ile Phe Gly Glu Glu Trp Gly Leu Pro
210 215 220
Thr Ala Thr Ile Asn Asn Tyr Tyr Lys Arg Gln Met Asn Leu Thr Ala
225 230 235 240
Gln Tyr Ser Asp His Cys Val Asn Trp Tyr Asn Thr Gly Leu Asp Arg
245 250 255
Leu Lys Gly Ser Asn Ala Lys Ser Trp Leu Ser Tyr His Arg Phe Arg
260 265 270
Arg Glu Met Thr Leu Met Val Leu Asp Leu Val Ala Leu Tyr Pro Asn
275 280 285
Tyr Asp Val Arg Thr Tyr Pro Ile Glu Thr Thr Val Gln Leu Ser Arg
290 295 300
Glu Val Tyr Thr Asp Pro Ile Gly Tyr Asn Pro Lys Asn Pro Glu Asn
305 310 315 320
Ser Gly Phe Gly Leu Pro Trp Ala Ser Leu Asn Ser His Asn Phe Thr
325 330 335
Gln Ile Glu Arg Glu Val Ile Arg Pro Pro His Ala Phe Asn Ile Leu
340 345 350
Asn Ser Val Glu Ile Gly Thr Ile Lys Gln Lys Leu Leu Phe Asn Asp
355 360 365
Lys Glu Tyr Ile Asn Tyr Trp Ser Gly His Leu Leu Glu Tyr Thr Tyr
370 375 380
Ala Asn Asp Ser Glu Ile Tyr Lys Lys Asp Tyr Gly Asn Leu Asn Thr
385 390 395 400
Phe Glu Lys Asn Lys Ile Asp Leu Lys Asp Lys Asp Ile Tyr Arg Ile
405 410 415
Asp Ser Thr Ala Val Cys Leu Ala Asn Phe Tyr Thr Tyr Ile Tyr Gly
420 425 430
Ser Thr Lys Ser Asn Phe His Met Val Asn Thr Asp Asn Leu Lys Pro
435 440 445
Val Ser Tyr Ser Tyr Ser Lys Thr His Thr Ala Ser Pro Ser Lys Ile
450 455 460
Gln Asn Tyr Asp Ser Glu Asn Ala Leu Pro Gln Glu Thr Thr Asp Glu
465 470 475 480
Pro Ser Tyr Gln Ser Tyr Asn His Arg Leu Ala His Ile Thr Thr Tyr
485 490 495
Pro Phe Trp His Thr Asn Gly Ser Asn Ser Gln Ser Gly Phe Leu Pro
500 505 510
Val Phe Val Trp Thr His Arg Ser Val Asp Leu Tyr Asn Phe Ile Asp
515 520 525
Ser Lys Lys Ile Thr Gln Ile Pro Ala Val Lys Gly Leu Gln Asn Tyr
530 535 540
Ile Gln Lys Gly Ala Asn Ile Glu Glu Gly Ser Gly Tyr Thr Gly Gly
545 550 555 560
Asp Val Val Thr Leu His Asn Phe Glu Ser Ser Pro Lys Leu Val His
565 570 575
Leu Tyr Ala Thr Ala Ala Glu Asn Ala Leu Ser Gln Lys Tyr Arg Val
580 585 590
Arg Val Arg Tyr Val Ser Glu Gly Asn Gly Glu Leu Tyr Met Arg Val
595 600 605
Lys Lys Ser Pro Asp Glu Phe Phe Gly Gln Thr Ala Ser Ala Lys Ile
610 615 620
Thr Gln Thr Lys Asn Ser Glu Glu Lys Leu Thr Ser Lys Asn Phe Lys
625 630 635 640
Tyr Thr Glu Phe Asp Glu Leu Val Ser Pro Leu Val Pro Lys Phe Ile
645 650 655
Ile Glu Ile Thr Ala Val Asn Met Leu Phe Cys Pro His Cys Lys Leu
660 665 670
Tyr Val Asp Lys Ile Glu Phe Ile Pro Val
675 680
<210> 38
<211> 760
<212> БЕЛОК
<213> Bacillus sp.
<400> 38
Leu Leu Arg Glu Tyr Ile Asn Ala Leu Arg Ile Leu Gln Lys Thr Pro
1 5 10 15
Gly Ile Phe Cys Arg Trp Glu Ser Arg Ile Pro Asn Lys Glu Asn Lys
20 25 30
Gly Glu Thr Gln Met Asn Pro Asp Gln Asn Lys Asn Glu Tyr Glu Ile
35 40 45
Val Asn Thr Ser Gln Asn Tyr Asp Asn Val Ala Asn Arg Tyr Pro Tyr
50 55 60
Ala Asn Asp Pro Asn Ile Lys Met Gln Asn Thr Asn Tyr Arg Asp Trp
65 70 75 80
Met Asn Phe Cys Thr Gly Asn Arg Ile Glu Asn Ile Ser Pro Thr Ser
85 90 95
Ala Arg Asp Val Ile Val Thr Ser Thr Asp Ile Ile Gly Arg Ile Leu
100 105 110
Gly Met Leu Gly Ile Pro Gly Ala Ser Glu Ile Leu Glu Leu Trp Asn
115 120 125
Ile Leu Phe Asn Ala Leu Trp Pro Ala Pro Asp Ala Gln Trp Val Ala
130 135 140
Tyr Ile Asn His Thr Gln Ala Leu Ile Gln Arg Glu Leu Gln Leu Phe
145 150 155 160
Ala Arg Glu Gln Ala Leu Arg Gln Leu Glu Gly Leu Gly Asn Asn Leu
165 170 175
Asn Leu Tyr Arg Glu Ala Phe Arg Glu Trp Glu Gln Asp Arg Asn Asn
180 185 190
Pro Ser Thr Arg Asn Arg Val Ile Asp Arg Phe Arg Ile Met Asp Ala
195 200 205
Phe Leu Thr Gln Tyr Ile Pro Val Phe Arg Ile Asn Asn Tyr Gln Val
210 215 220
Gln Leu Leu Ser Val Tyr Ala Gln Ala Ala Asn Ile His Leu Leu Leu
225 230 235 240
Leu Arg Asp Ala Thr Val Phe Gly Ser Asp Trp Gly Leu Thr Ser Thr
245 250 255
Asn Ile Asn Asp Ile Tyr Asn Arg Gln Met Asn Leu Ile Ala Glu Tyr
260 265 270
Thr Asn His Cys Thr Thr Trp Tyr Asn Asn Gly Leu Asn Glu Ala Arg
275 280 285
Asn Leu Ser Arg Ser Ser Trp Asp Thr Phe Asn Asn Tyr Arg Arg Asp
290 295 300
Met Thr Ile Met Val Leu Asp Leu Ile Ala Leu Phe Pro Thr Tyr Asp
305 310 315 320
Gln Arg Arg Tyr Pro Ile Thr Thr Lys Leu Glu Leu Thr Arg Glu Ile
325 330 335
Tyr Thr Pro Ala Ile Ala Ser Gln Asn Trp Ser Asn Phe Asn His Leu
340 345 350
Ser Pro Asn Ile Asp Phe Ala Phe Tyr Glu Arg Asn Leu Ile Arg Pro
355 360 365
Pro Ser Leu Phe Thr Trp Leu Asp Arg Thr Glu Met Phe Ser Arg Asn
370 375 380
Leu Ser Val Glu Val Ser Glu Val Trp Gly Gly His Ile Asn Tyr Phe
385 390 395 400
His His Thr Arg Ser Leu Ala Ala Ser Leu Arg Ser Asn Phe Ile Gly
405 410 415
Ser Asp Gln Arg Arg Val Ser Gln Tyr Asn Phe Asn Phe Val Gly Asp
420 425 430
Asp Ile Phe Arg Val Asp Ser Leu Val Gly Ser Asn Thr Val Gly Asn
435 440 445
Phe Phe Gly Val Ile Gln Gly Asn Phe His Leu Val Asn Arg Ser Ser
450 455 460
Ser Ser Thr Arg Ala Ile Pro Phe Thr Thr Arg Ser Thr Asn Pro Ser
465 470 475 480
Arg Arg Ser Ile Val Ser Glu Leu Pro Gly Glu Asn Ser Asn Ser Pro
485 490 495
Asn Phe Arg Asn Tyr Thr His Arg Leu Ser Trp Ile Ser Gly Ala Phe
500 505 510
Ile Gly Ser Asp Ile Ala Asn Val Leu Ala Tyr Gly Trp Thr His Arg
515 520 525
Ser Val Asp Pro Asn Asn Thr Ile Leu Pro Asn Arg Ile Thr Gln Ile
530 535 540
Pro Ala Val Lys Ala Thr Thr Ile Ser Asn Ala Thr Val Val Ser Gly
545 550 555 560
Pro Gly Ser Thr Gly Gly Asn Leu Val Arg Leu His Asn Gln Gly Arg
565 570 575
Phe Gln Ile Gln Met Asn Phe Pro Thr Pro Gln Thr Asn Tyr Arg Ile
580 585 590
Arg Ile Arg Tyr Ala Ser Ile Gly Ser Arg Val Leu His Val Gln Phe
595 600 605
Ser Gly Ala Asn His Gly Pro Thr Ile Ala Pro Thr Thr Val Ser Leu
610 615 620
Asp Asn Leu Arg Tyr Glu Asn Phe Ala Tyr Ile Asp Val Ile Gly Thr
625 630 635 640
Phe Ile Pro Ala Leu Gly Asn Tyr Val Thr Ile Val Asn Pro Asn Pro
645 650 655
Gly Asp Val Val Ile Asp Lys Ile Glu Phe Ile Pro Ile Gly Thr Phe
660 665 670
Ala Asn Gln Ser Leu Gly Glu Thr Glu Val His Asn Asn Asn Tyr Asn
675 680 685
Gln Ser Tyr Asn Asn Tyr Asp Gln Asn Met Asp Asn Met Tyr Pro Pro
690 695 700
Ser Tyr Asn Asn Val Tyr Glu Gln Asn Ser His Asp Ser Tyr Asp Ser
705 710 715 720
Gly Tyr Asn Asn Thr Tyr Glu Pro Asn Tyr Asp Cys Asn Cys Asn Gln
725 730 735
Lys Tyr Asn Asn Asn Tyr Asn Gln Asn Ser Gly Cys Thr Cys Asn Gln
740 745 750
Gly Tyr Asn Ser Asn Tyr Pro Lys
755 760
<210> 39
<211> 669
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 39
Leu Leu Arg Glu Tyr Ile Asn Ala Leu Arg Ile Leu Gln Lys Thr Pro
1 5 10 15
Gly Ile Phe Cys Arg Trp Glu Ser Arg Ile Pro Asn Lys Glu Asn Lys
20 25 30
Gly Glu Thr Gln Met Asn Pro Asp Gln Asn Lys Asn Glu Tyr Glu Ile
35 40 45
Val Asn Thr Ser Gln Asn Tyr Asp Asn Val Ala Asn Arg Tyr Pro Tyr
50 55 60
Ala Asn Asp Pro Asn Ile Lys Met Gln Asn Thr Asn Tyr Arg Asp Trp
65 70 75 80
Met Asn Phe Cys Thr Gly Asn Arg Ile Glu Asn Ile Ser Pro Thr Ser
85 90 95
Ala Arg Asp Val Ile Val Thr Ser Thr Asp Ile Ile Gly Arg Ile Leu
100 105 110
Gly Met Leu Gly Ile Pro Gly Ala Ser Glu Ile Leu Glu Leu Trp Asn
115 120 125
Ile Leu Phe Asn Ala Leu Trp Pro Ala Pro Asp Ala Gln Trp Val Ala
130 135 140
Tyr Ile Asn His Thr Gln Ala Leu Ile Gln Arg Glu Leu Gln Leu Phe
145 150 155 160
Ala Arg Glu Gln Ala Leu Arg Gln Leu Glu Gly Leu Gly Asn Asn Leu
165 170 175
Asn Leu Tyr Arg Glu Ala Phe Arg Glu Trp Glu Gln Asp Arg Asn Asn
180 185 190
Pro Ser Thr Arg Asn Arg Val Ile Asp Arg Phe Arg Ile Met Asp Ala
195 200 205
Phe Leu Thr Gln Tyr Ile Pro Val Phe Arg Ile Asn Asn Tyr Gln Val
210 215 220
Gln Leu Leu Ser Val Tyr Ala Gln Ala Ala Asn Ile His Leu Leu Leu
225 230 235 240
Leu Arg Asp Ala Thr Val Phe Gly Ser Asp Trp Gly Leu Thr Ser Thr
245 250 255
Asn Ile Asn Asp Ile Tyr Asn Arg Gln Met Asn Leu Ile Ala Glu Tyr
260 265 270
Thr Asn His Cys Thr Thr Trp Tyr Asn Asn Gly Leu Asn Glu Ala Arg
275 280 285
Asn Leu Ser Arg Ser Ser Trp Asp Thr Phe Asn Asn Tyr Arg Arg Asp
290 295 300
Met Thr Ile Met Val Leu Asp Leu Ile Ala Leu Phe Pro Thr Tyr Asp
305 310 315 320
Gln Arg Arg Tyr Pro Ile Thr Thr Lys Leu Glu Leu Thr Arg Glu Ile
325 330 335
Tyr Thr Pro Ala Ile Ala Ser Gln Asn Trp Ser Asn Phe Asn His Leu
340 345 350
Ser Pro Asn Ile Asp Phe Ala Phe Tyr Glu Arg Asn Leu Ile Arg Pro
355 360 365
Pro Ser Leu Phe Thr Trp Leu Asp Arg Thr Glu Met Phe Ser Arg Asn
370 375 380
Leu Ser Val Glu Val Ser Glu Val Trp Gly Gly His Ile Asn Tyr Phe
385 390 395 400
His His Thr Arg Ser Leu Ala Ala Ser Leu Arg Ser Asn Phe Ile Gly
405 410 415
Ser Asp Gln Arg Arg Val Ser Gln Tyr Asn Phe Asn Phe Val Gly Asp
420 425 430
Asp Ile Phe Arg Val Asp Ser Leu Val Gly Ser Asn Thr Val Gly Asn
435 440 445
Phe Phe Gly Val Ile Gln Gly Asn Phe His Leu Val Asn Arg Ser Ser
450 455 460
Ser Ser Thr Arg Ala Ile Pro Phe Thr Thr Arg Ser Thr Asn Pro Ser
465 470 475 480
Arg Arg Ser Ile Val Ser Glu Leu Pro Gly Glu Asn Ser Asn Ser Pro
485 490 495
Asn Phe Arg Asn Tyr Thr His Arg Leu Ser Trp Ile Ser Gly Ala Phe
500 505 510
Ile Gly Ser Asp Ile Ala Asn Val Leu Ala Tyr Gly Trp Thr His Arg
515 520 525
Ser Val Asp Pro Asn Asn Thr Ile Leu Pro Asn Arg Ile Thr Gln Ile
530 535 540
Pro Ala Val Lys Ala Thr Thr Ile Ser Asn Ala Thr Val Val Ser Gly
545 550 555 560
Pro Gly Ser Thr Gly Gly Asn Leu Val Arg Leu His Asn Gln Gly Arg
565 570 575
Phe Gln Ile Gln Met Asn Phe Pro Thr Pro Gln Thr Asn Tyr Arg Ile
580 585 590
Arg Ile Arg Tyr Ala Ser Ile Gly Ser Arg Val Leu His Val Gln Phe
595 600 605
Ser Gly Ala Asn His Gly Pro Thr Ile Ala Pro Thr Thr Val Ser Leu
610 615 620
Asp Asn Leu Arg Tyr Glu Asn Phe Ala Tyr Ile Asp Val Ile Gly Thr
625 630 635 640
Phe Ile Pro Ala Leu Gly Asn Tyr Val Thr Ile Val Asn Pro Asn Pro
645 650 655
Gly Asp Val Val Ile Asp Lys Ile Glu Phe Ile Pro Ile
660 665
<210> 40
<211> 296
<212> БЕЛОК
<213> Bacillus sp.
<400> 40
Met Ala Ile Val Asp Leu Asp Ala Tyr Leu Thr Ala Leu Ala Lys Lys
1 5 10 15
Val Phe Pro Ile Pro Gln Ile Ile Lys Gly Val Phe Thr Ser Glu Leu
20 25 30
Ser Ile Lys Asn Ala Glu Ser Tyr Gly Phe Glu Ile Lys Asn Ser Lys
35 40 45
Pro Gln Gly Thr Met Phe Met Gly Glu Ser Val Leu Lys Asn Asp Thr
50 55 60
Asn Glu Thr Gln Leu Ile His Ser Asp Ser Phe Thr Lys Thr Ile Thr
65 70 75 80
Asp Ser Val Thr Leu Ser Val Thr Asn Gly Ile Thr Thr Gly Val Asn
85 90 95
Ile Ser Ile Gly Gly Lys Val Phe Gly Met Gly Val Asp Thr Ser Met
100 105 110
Ser Phe Glu Val Ser Thr Ser Thr Thr Arg Glu Gln Thr Ser Thr Glu
115 120 125
Gly Val Ala Tyr Thr Val Pro Ser Gln Thr Val Ser Val Pro Ala Arg
130 135 140
Lys Thr Tyr Tyr Val Tyr Thr Ala Leu Gln Arg Ser Gln Leu Glu Gly
145 150 155 160
Ser Ile Arg Leu Arg Ala Asp Leu Ser Gly Asp Leu His Leu Lys Ile
165 170 175
Asp Ile Gly Gly Ser Asp Gly Ser Met Asn Val Gly Asn Ile Tyr Glu
180 185 190
Phe Ile Lys Thr His Gln Leu Ser His Pro Phe Pro Ser Gly Ile Ser
195 200 205
Leu Asn His Asn Asn Lys Ser Ile His Phe Glu Gly Ile Ala Glu Leu
210 215 220
Ile Tyr Gly Thr Gly Thr Lys Phe Asn Val Thr Ile Thr Asp Lys Pro
225 230 235 240
Ser Ser Gln Gly Thr Gln Glu His Thr Pro Ile Asp Ala Lys Thr Gly
245 250 255
Leu Gly Thr Tyr Glu Ile Gln Leu Asp Gly Lys Lys Leu Gly Phe Asp
260 265 270
Leu Asn Asp Leu Lys Asp Lys Thr Glu Thr Glu Val Phe Glu Lys Leu
275 280 285
Lys Glu Leu Gln Asn Glu Ile Val
290 295
<210> 41
<211> 649
<212> БЕЛОК
<213> Bacillus sp.
<400> 41
Met Lys Asn Thr Lys Leu Asp Thr Gln Asn Ser Leu Arg Tyr Pro Leu
1 5 10 15
Ala Asn Asp Gln Thr Thr Ala Leu Gln Asn Met Asn Tyr Lys Asp Tyr
20 25 30
Leu Arg Met Thr Lys Gly Gln Asn Pro Glu Leu Leu Gly Asn Pro Glu
35 40 45
Thr Leu Ile Ser Ser Ser Ser Val Gln Thr Gly Ile Gly Ile Val Gly
50 55 60
Lys Ile Leu Gly Ser Leu Gly Val Pro Phe Ala Gly Val Ile Ala Ser
65 70 75 80
Phe Tyr Ser Phe Ile Val Gly Gln Leu Trp Pro Ser Ser Thr Val Ser
85 90 95
Ile Trp Glu Met Ile Met Glu Gln Val Glu Glu Leu Val Asn Gln Lys
100 105 110
Ile Thr Glu His Ala Arg Glu Gln Ala Leu Ala Lys Leu Arg Gly Leu
115 120 125
Gly Asp Gly Leu Asp Val Tyr Gln Gln Ser Leu Glu Asn Trp Leu Lys
130 135 140
Asn Arg Asn Asp Thr Arg Ala Arg Ser Val Val Ala Thr Gln Phe Ile
145 150 155 160
Ala Leu Glu Leu Asp Phe Val Ser Ser Ile Pro Ser Phe Ala Val Ala
165 170 175
Gly Gln Glu Val Pro Leu Leu Ala Ile Tyr Ala Gln Ala Ala Asn Leu
180 185 190
His Leu Leu Leu Leu Arg Asp Ala Ser Ile Phe Gly Glu Glu Trp Gly
195 200 205
Phe Thr Ser Gly Glu Ile Ser Thr Phe Tyr Asn Arg Gln Val Thr Arg
210 215 220
Thr Ala His Tyr Ser Asp Tyr Cys Val Ser Trp Tyr Asn Phe Gly Leu
225 230 235 240
Asp Arg Leu Lys Asp Thr Ser Ala Ala Ser Trp Leu Arg Tyr His Gln
245 250 255
Phe Arg Arg Glu Met Thr Leu Leu Val Leu Asp Leu Val Ser Leu Phe
260 265 270
Pro Ser Tyr Asp Thr Arg Thr Tyr Pro Ile Glu Thr Thr Ala Gln Leu
275 280 285
Thr Arg Asp Val Tyr Thr Asp Pro Leu Ala Phe Asn Lys Val Thr Ser
290 295 300
Ser Gly Phe Cys Asn Pro Trp Ser Thr His Ser Gly Val Leu Phe Ser
305 310 315 320
Glu Val Glu Ser Ala Val Ile Arg Pro Pro His Leu Phe Asp Ile Leu
325 330 335
Ser Ser Val Glu Ile Ser Thr Ala Arg Gly Lys Ile Ala Leu Asn Asn
340 345 350
Thr Ala Tyr Leu Asp Tyr Trp Ile Gly His Ser Leu Lys Tyr His Asn
355 360 365
Ala Asn Asn Thr Ser Leu Leu Gln Ser Asn Tyr Gly Arg Ile Thr Ser
370 375 380
Glu Lys Asn Ser Phe Thr Leu Glu Asp Lys Asp Ile Phe Glu Ile Asn
385 390 395 400
Ser Thr Thr Ala Asn Leu Ala Asn Thr Tyr Gln Lys Ala Tyr Gly Val
405 410 415
Pro Glu Ser Trp Phe Cys Met Ile Asn Arg Asp Ser Ser Val Thr Ser
420 425 430
Val Tyr Cys Tyr Ser Gln Thr His Thr Thr Leu Ser Gly Cys Thr Gln
435 440 445
Val Tyr Glu Ser Ser Asp Glu Ile Pro Leu Asp Arg Thr Val Thr Val
450 455 460
Ala Gly Ser Tyr Ser His Arg Leu Ser His Ile Thr Tyr His Ser Phe
465 470 475 480
Ser Lys His Ser Ser Lys Tyr Tyr Gly Gly Phe Pro Val Phe Val Trp
485 490 495
Ser His Val Ser Ala Asp Leu Asn Asn Thr Ile Tyr Ala Asp Lys Ile
500 505 510
Thr Gln Leu Pro Leu Thr Lys Ser Asp Glu Ile Asn Asp Asn Val Glu
515 520 525
Ile Val Asn Ser Asp Tyr Val Gly Gly Ser Val Leu Lys Val Cys Arg
530 535 540
Glu Asn Leu Leu Ser Asn Arg Val Asn Arg Ile Gln Phe Ser Leu Pro
545 550 555 560
Leu Ser Glu Ser Lys Arg Tyr Glu Val Arg Val Arg Tyr Ala Ser Thr
565 570 575
Gly Asp Gly Phe Met Gly Met Thr Asn Val Ser Gly Asp Lys Arg Phe
580 585 590
Ser Ala Thr Met Glu Glu Gly Ser Asp Leu Lys Tyr Asp Ser Phe Arg
595 600 605
Tyr Ile Thr Leu Gly Ser Leu Arg Glu Pro Ser Ile Asn Asn Tyr Tyr
610 615 620
Ser Leu Tyr Leu Pro Thr Pro Tyr Val Glu Lys Tyr Thr Ile Tyr Ile
625 630 635 640
Asp Arg Ile Glu Ile Ile Pro Val Ser
645
<210> 42
<211> 648
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 42
Met Lys Asn Thr Lys Leu Asp Thr Gln Asn Ser Leu Arg Tyr Pro Leu
1 5 10 15
Ala Asn Asp Gln Thr Thr Ala Leu Gln Asn Met Asn Tyr Lys Asp Tyr
20 25 30
Leu Arg Met Thr Lys Gly Gln Asn Pro Glu Leu Leu Gly Asn Pro Glu
35 40 45
Thr Leu Ile Ser Ser Ser Ser Val Gln Thr Gly Ile Gly Ile Val Gly
50 55 60
Lys Ile Leu Gly Ser Leu Gly Val Pro Phe Ala Gly Val Ile Ala Ser
65 70 75 80
Phe Tyr Ser Phe Ile Val Gly Gln Leu Trp Pro Ser Ser Thr Val Ser
85 90 95
Ile Trp Glu Met Ile Met Glu Gln Val Glu Glu Leu Val Asn Gln Lys
100 105 110
Ile Thr Glu His Ala Arg Glu Gln Ala Leu Ala Lys Leu Arg Gly Leu
115 120 125
Gly Asp Gly Leu Asp Val Tyr Gln Gln Ser Leu Glu Asn Trp Leu Lys
130 135 140
Asn Arg Asn Asp Thr Arg Ala Arg Ser Val Val Ala Thr Gln Phe Ile
145 150 155 160
Ala Leu Glu Leu Asp Phe Val Ser Ser Ile Pro Ser Phe Ala Val Ala
165 170 175
Gly Gln Glu Val Pro Leu Leu Ala Ile Tyr Ala Gln Ala Ala Asn Leu
180 185 190
His Leu Leu Leu Leu Arg Asp Ala Ser Ile Phe Gly Glu Glu Trp Gly
195 200 205
Phe Thr Ser Gly Glu Ile Ser Thr Phe Tyr Asn Arg Gln Val Thr Arg
210 215 220
Thr Ala His Tyr Ser Asp Tyr Cys Val Ser Trp Tyr Asn Phe Gly Leu
225 230 235 240
Asp Arg Leu Lys Asp Thr Ser Ala Ala Ser Trp Leu Arg Tyr His Gln
245 250 255
Phe Arg Arg Glu Met Thr Leu Leu Val Leu Asp Leu Val Ser Leu Phe
260 265 270
Pro Ser Tyr Asp Thr Arg Thr Tyr Pro Ile Glu Thr Thr Ala Gln Leu
275 280 285
Thr Arg Asp Val Tyr Thr Asp Pro Leu Ala Phe Asn Lys Val Thr Ser
290 295 300
Ser Gly Phe Cys Asn Pro Trp Ser Thr His Ser Gly Val Leu Phe Ser
305 310 315 320
Glu Val Glu Ser Ala Val Ile Arg Pro Pro His Leu Phe Asp Ile Leu
325 330 335
Ser Ser Val Glu Ile Ser Thr Ala Arg Gly Lys Ile Ala Leu Asn Asn
340 345 350
Thr Ala Tyr Leu Asp Tyr Trp Ile Gly His Ser Leu Lys Tyr His Asn
355 360 365
Ala Asn Asn Thr Ser Leu Leu Gln Ser Asn Tyr Gly Arg Ile Thr Ser
370 375 380
Glu Lys Asn Ser Phe Thr Leu Glu Asp Lys Asp Ile Phe Glu Ile Asn
385 390 395 400
Ser Thr Thr Ala Asn Leu Ala Asn Thr Tyr Gln Lys Ala Tyr Gly Val
405 410 415
Pro Glu Ser Trp Phe Cys Met Ile Asn Arg Asp Ser Ser Val Thr Ser
420 425 430
Val Tyr Cys Tyr Ser Gln Thr His Thr Thr Leu Ser Gly Cys Thr Gln
435 440 445
Val Tyr Glu Ser Ser Asp Glu Ile Pro Leu Asp Arg Thr Val Thr Val
450 455 460
Ala Gly Ser Tyr Ser His Arg Leu Ser His Ile Thr Tyr His Ser Phe
465 470 475 480
Ser Lys His Ser Ser Lys Tyr Tyr Gly Gly Phe Pro Val Phe Val Trp
485 490 495
Ser His Val Ser Ala Asp Leu Asn Asn Thr Ile Tyr Ala Asp Lys Ile
500 505 510
Thr Gln Leu Pro Leu Thr Lys Ser Asp Glu Ile Asn Asp Asn Val Glu
515 520 525
Ile Val Asn Ser Asp Tyr Val Gly Gly Ser Val Leu Lys Val Cys Arg
530 535 540
Glu Asn Leu Leu Ser Asn Arg Val Asn Arg Ile Gln Phe Ser Leu Pro
545 550 555 560
Leu Ser Glu Ser Lys Arg Tyr Glu Val Arg Val Arg Tyr Ala Ser Thr
565 570 575
Gly Asp Gly Phe Met Gly Met Thr Asn Val Ser Gly Asp Lys Arg Phe
580 585 590
Ser Ala Thr Met Glu Glu Gly Ser Asp Leu Lys Tyr Asp Ser Phe Arg
595 600 605
Tyr Ile Thr Leu Gly Ser Leu Arg Glu Pro Ser Ile Asn Asn Tyr Tyr
610 615 620
Ser Leu Tyr Leu Pro Thr Pro Tyr Val Glu Lys Tyr Thr Ile Tyr Ile
625 630 635 640
Asp Arg Ile Glu Ile Ile Pro Val
645
<210> 43
<211> 395
<212> БЕЛОК
<213> Bacillus sp.
<400> 43
Met Val Met Gln Met Val Thr Lys Ala Met Thr Thr Lys Leu Gly Ala
1 5 10 15
Leu Pro Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Asp
20 25 30
Ser Ile Thr Gly Val Met Ser Thr Ile Phe Gly Ile Asp Thr Gly Asp
35 40 45
Ser Ser Ile Asp Asp Val Leu Asn Asn Gln Lys Leu Leu Gln Glu Met
50 55 60
Gln Ala Gln Met Gln Gln Met Gln Asp Ser Ile Thr Asp Met Ala Asn
65 70 75 80
Gln Gln Met Ile Ser Glu Asp Ile Glu Lys Gln Ile Leu Ala Leu Thr
85 90 95
Thr Glu Leu Ser Ala Ser Ile Thr Thr Glu Phe Asn Lys Ile Glu Gly
100 105 110
Ile Leu Asn Thr Tyr Leu Pro Ala Ile Ser Lys Met Leu Gly Glu Val
115 120 125
Tyr Glu Gln Thr Ser Glu Ile Asn Glu Lys Val Asp Lys Leu Leu Ala
130 135 140
Met Met Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val
145 150 155 160
Val Leu Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu
165 170 175
Asp Tyr Val Asn Lys Lys Phe Leu Ser Leu Thr Lys Ser Phe Leu Gln
180 185 190
Asn Glu Asp Leu Ser Ile Asp Ser Met Gln Glu Met Leu Glu Trp Ala
195 200 205
Lys Ser Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp
210 215 220
Thr Leu His Ser Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala
225 230 235 240
Leu Lys Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly
245 250 255
Asp Phe Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu
260 265 270
Ala Thr Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys
275 280 285
Ile Leu Gly Leu Ser Glu Phe Asp Tyr Leu Val Thr Met Glu Asp Arg
290 295 300
Ile Glu Lys Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Met Asn
305 310 315 320
Tyr Ser Asn Ala Leu Glu Ile Asn Gly Ile Tyr Pro Ile Pro Ser Arg
325 330 335
Trp Gly Glu Cys Lys Ser Leu Asp Leu Gln Ala Glu Ser Gly Tyr Ala
340 345 350
Ile Ile Gly Leu Glu Phe Phe Met Glu Asn Gly Ile Tyr Lys Ala Lys
355 360 365
Ala Tyr Gln Gly Glu Ile Gly Lys Asn Phe Ser Val Ser Ala Asp Thr
370 375 380
Val Ser Glu Ile Ile Ser Asp Asp Phe Lys Phe
385 390 395
<210> 44
<211> 291
<212> БЕЛОК
<213> Bacillus sp.
<400> 44
Leu Ile Arg Asn Tyr Ser Ile Lys Lys Glu Arg Phe Tyr Met Thr Thr
1 5 10 15
Lys Asn Leu Ser Glu Leu Glu Met Glu Ile Ser Asn Asn Gln Gln Gln
20 25 30
Pro Arg Arg Leu Ile Val Pro Ala Phe Arg His Ile Val Leu Lys Val
35 40 45
Pro Ala Ser Glu Glu Ser Asn Tyr Lys Glu Ile Phe Gln Val Glu Pro
50 55 60
Arg Tyr Ile Ala Gln Ala Leu Arg Leu Ala Asn Ala Phe Gln Gly Ala
65 70 75 80
Leu Asp Pro Thr Asp Leu Asn Phe Asn Phe Glu Lys Ala Leu Gln Ile
85 90 95
Ala Gln His Ile Pro Asn Ser Ala Ile Val Lys Thr Leu Asn Gln Asn
100 105 110
Val Val Gln Gln Thr Val Glu Ile Ser Ala Met Ile Ser Gln Leu Lys
115 120 125
Gln Ile Ile Arg Asp Val Leu Gly Leu Val Ile Ser Ser Pro Asn Phe
130 135 140
Trp Asn Ser Val Glu Ala Ala Val Thr Asn Thr Phe Thr Asn Leu Asn
145 150 155 160
Thr Gln Glu Asp Gly Ala Trp Ile Phe Trp Lys Ser Ser Ser Ser Thr
165 170 175
Asn Thr Ser Tyr Tyr Tyr Asn Ile Leu Phe Ser Ile Gln Asn Ala Glu
180 185 190
Thr Gly Ser Val Met Ala Val Leu Pro Ile Ala Phe Glu Ile Ser Val
195 200 205
Asn Leu Glu Lys Gln Lys Val Leu Phe Leu Thr Ile Arg Asp Ser Ala
210 215 220
Arg Tyr Glu Val Lys Leu Lys Ala Leu Thr Leu Val Gln Ala Leu Asp
225 230 235 240
Ser Phe Asn Ala Pro Ile Glu Asp Val Phe Asn Val His Asn Tyr Gly
245 250 255
Pro Tyr Pro Pro Asn Asn Ser Ser Thr Asn Asn Tyr Ile Leu Gln Asn
260 265 270
Leu Lys Phe Asn Gln Ser Ser Thr Asn Asn Leu Ile Val Gln Asp Thr
275 280 285
Ile Asp Asn
290
<210> 45
<211> 258
<212> БЕЛОК
<213> Lysinbacillus sp.
<400> 45
Met Thr Ile Gln Glu Lys Leu Ser Phe Thr Asp Leu Pro Ala Leu Lys
1 5 10 15
Ser Ser Pro Glu Ser Val Arg Gln Ala Phe Thr Asn Lys Phe Gly Thr
20 25 30
Lys Pro Asp Gly Ile Ser Val Asn Ser Glu Thr Tyr Phe Asn Ala Val
35 40 45
Lys Pro Ala Ile Thr Glu Gln Tyr Gly His Pro Cys Tyr Lys Arg Leu
50 55 60
Gly Asp Phe Asn Tyr Met Lys Gly Asp Gly Lys Pro Pro Thr Ser Ala
65 70 75 80
Ile Val Gly Ser Asn Val Ala Val Asn Tyr Gly Asp Glu Glu Ala Thr
85 90 95
Met Thr Leu Glu Val Gln Gly Ser Trp Gln Ser Glu Gln Ser Trp Ser
100 105 110
Ser Glu Ser Thr Thr Gly Leu Thr Ile Ser Ser Lys Phe Thr Ile Glu
115 120 125
Gly Phe Phe Glu Ser Gly Met Glu Phe Ser Val Ser Thr Thr Val Gly
130 135 140
Glu Thr Lys Thr Glu Ser Glu Ser Arg Thr Ala Thr Ala Ser Val Gln
145 150 155 160
Val Thr Val Pro Pro Arg Ser Lys Lys Gln Val Thr Met Val Gly Thr
165 170 175
Leu Lys Lys Glu Ser Met Asn Phe Arg Ala Pro Ile Ser Val Asp Gly
180 185 190
Met Phe Gly Ala Asn Phe Pro Lys Arg Val Gln Asp His Tyr Phe Trp
195 200 205
Phe Leu Gly Ala Gly Ser Val Leu Thr Gln Thr Thr Gly Glu Ile Thr
210 215 220
Gly Thr Ile Lys Asn Thr Ala Val Phe Asp Val His Thr Glu Ile Gly
225 230 235 240
Lys Thr Glu Pro Leu Thr Ala Glu Glu Leu Ser Lys Leu Ala Val Leu
245 250 255
Ala Lys
<210> 46
<211> 950
<212> БЕЛОК
<213> Bacillus sp.
<400> 46
Met Ile Met Gln Met Val Thr Lys Ala Met Thr Pro Lys Leu Gly Ala
1 5 10 15
Leu Pro Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Gly
20 25 30
Asn Ile Thr Asp Val Met Ser Thr Ile Phe Gly Val Asp Thr Gly Asp
35 40 45
Ser Ser Ile Asp Asp Val Leu Asn Asn Ala Glu Leu Leu Gln Glu Met
50 55 60
Leu Asp Gln Met Asn Thr Met Gln Ile Ser Ile Arg Asp Ile Leu Glu
65 70 75 80
Gln Gln Gly Ile Ser Ala Asp Ile Glu Lys Gln Ile Leu Ala Leu Thr
85 90 95
Thr Asp Leu Ala Thr Ser Ile Asn Thr Glu Leu Gly Lys Ile Glu Gly
100 105 110
Ile Leu Asn Thr Tyr Leu Pro Ala Ile Ser Asn Met Leu Ser Asn Ile
115 120 125
Tyr Glu Gln Thr Ser Leu Ile Asp Gln Lys Val Asp Lys Leu Leu Ala
130 135 140
Leu Met Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val
145 150 155 160
Val Leu Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu
165 170 175
Val Tyr Val Asn Lys Lys Phe Leu Ser Leu Thr Arg Gly Phe Leu Gln
180 185 190
Gly Glu Asp Val Ser Ile Ala Ser Met Gln Glu Ile Gln Glu Trp Ala
195 200 205
Lys Ser Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp
210 215 220
Thr Leu His Asn Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala
225 230 235 240
Leu Lys Ser Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly
245 250 255
Asp Phe Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu
260 265 270
Ala Thr Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys
275 280 285
Val Leu Gly Ile Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg
290 295 300
Ile Glu Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn
305 310 315 320
Tyr Ser Asn Ala Leu Glu Ile Thr Gly Ile Tyr Pro Met Leu Val Asp
325 330 335
Arg Gly Asp Cys Lys Ser Thr Asp Leu Gln Ala Asp Ala Gly Tyr Ala
340 345 350
Leu Ile Gly Leu Glu Phe Phe Met Asp Asn Gly Ile Tyr Lys Ala Lys
355 360 365
Ala Tyr Gln Gly Lys Ile Trp Lys Asn Phe Ser Val Ser Ala Asp Ser
370 375 380
Val Thr Glu Ile Ile Ser Asp Asp Leu Ser Thr Val Phe His Asp Met
385 390 395 400
Thr Ser Asp Lys Leu Asp Val Val Tyr Pro Leu Ser Gly Glu Leu Thr
405 410 415
Gly Pro Pro Asn Thr Val Ile Thr Arg Ile Gly Phe Gly Thr Lys Tyr
420 425 430
Asp Lys Thr Arg Glu Ser Gly Val Gln Ala Phe Ala Tyr Val Asp Ala
435 440 445
Asp Phe Ser Pro Tyr Asp Tyr Ile Ser Gly Thr Ile Ser Lys Glu Gly
450 455 460
Thr Gln Thr Ile Ser Leu Glu Gly Asn Asp Pro Lys Asn Arg Cys Tyr
465 470 475 480
Ser Met Trp Pro Ile Gly Leu Ile Gly Asp Leu Tyr Met Thr Pro Leu
485 490 495
Lys Ser Leu Ser Leu Asn Val Asp Val Asp Asp Ser Ser Ala Tyr Gly
500 505 510
Thr Ile Leu Asn Met Ser Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser
515 520 525
Arg Glu Tyr Asn Thr Asn Phe Ile Leu Phe Pro Tyr Thr Asn Asn Ser
530 535 540
Gly Leu Ile Asp Glu Asn Leu Ile Gln Asn Gly Asn Leu Glu Glu Gln
545 550 555 560
Asp Lys Tyr Trp Glu Gly Thr Glu Pro Ser Phe Phe Ala Glu Gly Glu
565 570 575
Gly Thr Phe Gly Ser Asn Ala Leu Lys Val Gln Ala Gln Gly Ala Phe
580 585 590
Asp Gln Ile Val Lys Leu Glu Pro Asn Thr Thr Tyr Arg Leu Thr Ala
595 600 605
Phe Thr Lys Val Lys Asp Ser Asn Tyr Ser Lys Gly Arg Ile Gly Ile
610 615 620
Arg Asp Met Tyr Asn Tyr Arg Val Glu Lys Ser Phe Leu His Thr Arg
625 630 635 640
Tyr Asn Gln Thr Lys Val Glu Phe Thr Thr Gly Ala Asp Thr Ser Lys
645 650 655
Leu Ser Val Phe Ile Gln Gly Gly Gly Asp Gln Glu Ser Ile Ala Trp
660 665 670
Ala Asp Asn Ile Glu Leu Tyr Lys Val His Gln Gln Asp Asn Met Ile
675 680 685
Ser Asp Phe Asp Phe Asp Thr Tyr Lys Tyr Ser Asn Met Cys Trp Lys
690 695 700
Leu Ser Gly Gly Gly Asp Phe Val Glu Lys Glu Gly Leu Phe Asn Ser
705 710 715 720
Lys Ala Leu Lys Ile Thr Asn Ser Gly Gly Ala Glu Gln Glu Val Lys
725 730 735
Leu Lys Ala Asn Thr Asn Tyr Ile Leu Thr Ala Tyr Val Lys Val Asp
740 745 750
Val Pro Thr Thr Thr Ala Gln Ile Gly Cys Gly Ser Asn Gln Val Pro
755 760 765
Cys Asn Ser Thr Ser Tyr Thr Pro Val Lys Leu Lys Phe Lys Thr Gly
770 775 780
Glu Asp Pro Ser Thr Thr Glu Ser Ser Val Tyr Cys Ser Asn Ser Asn
785 790 795 800
Asp Ser Gly Thr Val Trp Ala Asp Asn Phe Val Leu Tyr Glu Ala Pro
805 810 815
Asn Leu Ile Ile Asn Gly Asp Phe Glu Gln Phe Asp Gln Ser Ser Trp
820 825 830
Asn Phe Ser Pro Ser Val Gly Gly Arg Ile Tyr Leu Gln Ser Gly Leu
835 840 845
Gly Met Ser His Ser Asn Ala Val Val Ile His Gly Glu Asn Gly Lys
850 855 860
Ile Ser Gln Lys Ile Pro Leu Lys Pro Phe Thr Lys Tyr Arg Leu Thr
865 870 875 880
Ala Tyr Val Arg Val Ser Lys Gly Ser Leu Ala Tyr Ile Gly Tyr Gly
885 890 895
Glu Tyr Asn Cys Ala Cys Ala Val Asp Asp Phe Arg Gln Ala His Val
900 905 910
Glu Phe Thr Thr Gly Ala Asn Pro Met Gln Ser Asp Asp Ala Ile Tyr
915 920 925
Leu Asp Ser Phe Ala Ser Gly Tyr Thr Val Ala Asp Asn Phe Glu Leu
930 935 940
Tyr Glu Leu Asp Gln Ile
945 950
<210> 47
<211> 948
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 47
Met Gln Met Val Thr Lys Ala Met Thr Pro Lys Leu Gly Ala Leu Pro
1 5 10 15
Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Gly Asn Ile
20 25 30
Thr Asp Val Met Ser Thr Ile Phe Gly Val Asp Thr Gly Asp Ser Ser
35 40 45
Ile Asp Asp Val Leu Asn Asn Ala Glu Leu Leu Gln Glu Met Leu Asp
50 55 60
Gln Met Asn Thr Met Gln Ile Ser Ile Arg Asp Ile Leu Glu Gln Gln
65 70 75 80
Gly Ile Ser Ala Asp Ile Glu Lys Gln Ile Leu Ala Leu Thr Thr Asp
85 90 95
Leu Ala Thr Ser Ile Asn Thr Glu Leu Gly Lys Ile Glu Gly Ile Leu
100 105 110
Asn Thr Tyr Leu Pro Ala Ile Ser Asn Met Leu Ser Asn Ile Tyr Glu
115 120 125
Gln Thr Ser Leu Ile Asp Gln Lys Val Asp Lys Leu Leu Ala Leu Met
130 135 140
Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val Val Leu
145 150 155 160
Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu Val Tyr
165 170 175
Val Asn Lys Lys Phe Leu Ser Leu Thr Arg Gly Phe Leu Gln Gly Glu
180 185 190
Asp Val Ser Ile Ala Ser Met Gln Glu Ile Gln Glu Trp Ala Lys Ser
195 200 205
Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp Thr Leu
210 215 220
His Asn Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala Leu Lys
225 230 235 240
Ser Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly Asp Phe
245 250 255
Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu Ala Thr
260 265 270
Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys Val Leu
275 280 285
Gly Ile Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg Ile Glu
290 295 300
Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn Tyr Ser
305 310 315 320
Asn Ala Leu Glu Ile Thr Gly Ile Tyr Pro Met Leu Val Asp Arg Gly
325 330 335
Asp Cys Lys Ser Thr Asp Leu Gln Ala Asp Ala Gly Tyr Ala Leu Ile
340 345 350
Gly Leu Glu Phe Phe Met Asp Asn Gly Ile Tyr Lys Ala Lys Ala Tyr
355 360 365
Gln Gly Lys Ile Trp Lys Asn Phe Ser Val Ser Ala Asp Ser Val Thr
370 375 380
Glu Ile Ile Ser Asp Asp Leu Ser Thr Val Phe His Asp Met Thr Ser
385 390 395 400
Asp Lys Leu Asp Val Val Tyr Pro Leu Ser Gly Glu Leu Thr Gly Pro
405 410 415
Pro Asn Thr Val Ile Thr Arg Ile Gly Phe Gly Thr Lys Tyr Asp Lys
420 425 430
Thr Arg Glu Ser Gly Val Gln Ala Phe Ala Tyr Val Asp Ala Asp Phe
435 440 445
Ser Pro Tyr Asp Tyr Ile Ser Gly Thr Ile Ser Lys Glu Gly Thr Gln
450 455 460
Thr Ile Ser Leu Glu Gly Asn Asp Pro Lys Asn Arg Cys Tyr Ser Met
465 470 475 480
Trp Pro Ile Gly Leu Ile Gly Asp Leu Tyr Met Thr Pro Leu Lys Ser
485 490 495
Leu Ser Leu Asn Val Asp Val Asp Asp Ser Ser Ala Tyr Gly Thr Ile
500 505 510
Leu Asn Met Ser Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser Arg Glu
515 520 525
Tyr Asn Thr Asn Phe Ile Leu Phe Pro Tyr Thr Asn Asn Ser Gly Leu
530 535 540
Ile Asp Glu Asn Leu Ile Gln Asn Gly Asn Leu Glu Glu Gln Asp Lys
545 550 555 560
Tyr Trp Glu Gly Thr Glu Pro Ser Phe Phe Ala Glu Gly Glu Gly Thr
565 570 575
Phe Gly Ser Asn Ala Leu Lys Val Gln Ala Gln Gly Ala Phe Asp Gln
580 585 590
Ile Val Lys Leu Glu Pro Asn Thr Thr Tyr Arg Leu Thr Ala Phe Thr
595 600 605
Lys Val Lys Asp Ser Asn Tyr Ser Lys Gly Arg Ile Gly Ile Arg Asp
610 615 620
Met Tyr Asn Tyr Arg Val Glu Lys Ser Phe Leu His Thr Arg Tyr Asn
625 630 635 640
Gln Thr Lys Val Glu Phe Thr Thr Gly Ala Asp Thr Ser Lys Leu Ser
645 650 655
Val Phe Ile Gln Gly Gly Gly Asp Gln Glu Ser Ile Ala Trp Ala Asp
660 665 670
Asn Ile Glu Leu Tyr Lys Val His Gln Gln Asp Asn Met Ile Ser Asp
675 680 685
Phe Asp Phe Asp Thr Tyr Lys Tyr Ser Asn Met Cys Trp Lys Leu Ser
690 695 700
Gly Gly Gly Asp Phe Val Glu Lys Glu Gly Leu Phe Asn Ser Lys Ala
705 710 715 720
Leu Lys Ile Thr Asn Ser Gly Gly Ala Glu Gln Glu Val Lys Leu Lys
725 730 735
Ala Asn Thr Asn Tyr Ile Leu Thr Ala Tyr Val Lys Val Asp Val Pro
740 745 750
Thr Thr Thr Ala Gln Ile Gly Cys Gly Ser Asn Gln Val Pro Cys Asn
755 760 765
Ser Thr Ser Tyr Thr Pro Val Lys Leu Lys Phe Lys Thr Gly Glu Asp
770 775 780
Pro Ser Thr Thr Glu Ser Ser Val Tyr Cys Ser Asn Ser Asn Asp Ser
785 790 795 800
Gly Thr Val Trp Ala Asp Asn Phe Val Leu Tyr Glu Ala Pro Asn Leu
805 810 815
Ile Ile Asn Gly Asp Phe Glu Gln Phe Asp Gln Ser Ser Trp Asn Phe
820 825 830
Ser Pro Ser Val Gly Gly Arg Ile Tyr Leu Gln Ser Gly Leu Gly Met
835 840 845
Ser His Ser Asn Ala Val Val Ile His Gly Glu Asn Gly Lys Ile Ser
850 855 860
Gln Lys Ile Pro Leu Lys Pro Phe Thr Lys Tyr Arg Leu Thr Ala Tyr
865 870 875 880
Val Arg Val Ser Lys Gly Ser Leu Ala Tyr Ile Gly Tyr Gly Glu Tyr
885 890 895
Asn Cys Ala Cys Ala Val Asp Asp Phe Arg Gln Ala His Val Glu Phe
900 905 910
Thr Thr Gly Ala Asn Pro Met Gln Ser Asp Asp Ala Ile Tyr Leu Asp
915 920 925
Ser Phe Ala Ser Gly Tyr Thr Val Ala Asp Asn Phe Glu Leu Tyr Glu
930 935 940
Leu Asp Gln Ile
945
<210> 48
<211> 951
<212> БЕЛОК
<213> Bacillus sp.
<400> 48
Met Ile Met Gln Met Val Thr Lys Ala Met Thr Thr Lys Leu Gly Ala
1 5 10 15
Leu Pro Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Asp
20 25 30
Ser Ile Thr Gly Val Met Ser Thr Ile Phe Gly Ile Asp Thr Gly Asp
35 40 45
Ser Ser Ile Asp Asp Val Leu Asn Asn Gln Glu Leu Leu Gln Glu Met
50 55 60
Gln Ala Gln Met Gln Gln Met Gln Asp Ser Ile Thr Asp Met Ala Asn
65 70 75 80
Gln Gln Ile Ile Ser Glu Gly Ile Glu Lys Gln Ile Leu Ala Phe Thr
85 90 95
Thr Asp Leu Ser Ala Ser Ile Thr Thr Glu Phe Lys Lys Ile Glu Gly
100 105 110
Ile Leu Asn Thr Tyr Leu Pro Ala Ile Ser Asn Met Leu Ser Asn Ile
115 120 125
Tyr Glu Gln Thr Ser Val Ile Asp Gln Lys Val Asp Lys Leu Leu Ala
130 135 140
Leu Met Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val
145 150 155 160
Val Leu Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu
165 170 175
Val Tyr Val Asn Lys Lys Phe Leu Ser Leu Thr Arg Gly Phe Leu Gln
180 185 190
Gly Glu Asp Val Ser Ile Asp Asn Met Gln Glu Ile Gln Glu Trp Ala
195 200 205
Lys Ser Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp
210 215 220
Thr Leu His Asn Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala
225 230 235 240
Leu Lys Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly
245 250 255
Asp Phe Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu
260 265 270
Ala Thr Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys
275 280 285
Val Leu Gly Leu Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg
290 295 300
Ile Glu Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn
305 310 315 320
Tyr Ser Asn Ala Leu Glu Ile Thr Gly Ile Tyr Pro Met Ser Leu Asp
325 330 335
Trp Gly Asn Cys Lys Ser Thr Asp Leu Gln Ala Asp Asp Gly Tyr Ala
340 345 350
Leu Ile Gly Leu Glu Phe Phe Met Asp Asn Gly Ile Tyr Lys Ala Lys
355 360 365
Ala Tyr Gln Gly Lys Ile Gly Lys Asn Phe Ser Val Ser Ala Asp Thr
370 375 380
Val Thr Glu Ile Ile Ser Asp Asp Leu Ser Thr Val Phe Asn Asp Met
385 390 395 400
Thr Asn Asp Asn Pro Glu Leu Tyr Val Val Tyr Pro Leu Ser Gly Glu
405 410 415
Leu Thr Gly Pro Pro Asn Thr Ile Ile Thr Arg Ile Gly Phe Gly Thr
420 425 430
Lys Tyr Asp Lys Thr Arg Glu Ser Ser Val Gln Ala Phe Ala Tyr Ile
435 440 445
Asp Ala Asp Phe Ser Pro Tyr Asp Tyr Ile Ser Gly Thr Ile Ser Lys
450 455 460
Glu Gly Thr Gln Thr Ile Ser Leu Glu Gly Asn Asp Pro Lys Asn Gln
465 470 475 480
Cys Tyr Ser Met Trp Pro Ile Gly Leu Ile Gly Asp Leu Tyr Met Thr
485 490 495
Pro Leu Lys Ser Leu Ser Leu Asn Val Asp Asp Ser Ser Ala Tyr Gly
500 505 510
Thr Ile Leu Asn Met Ser Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser
515 520 525
Arg Glu Tyr Asn Thr Asn Phe Ile Leu Phe Pro Tyr Thr Asn Asn Ser
530 535 540
Ser Pro Ile Val Glu Asn Leu Ile Gln Asn Gly Asp Phe Glu Asp Gly
545 550 555 560
Asp Lys Tyr Trp Glu Val Val Ser Gly Thr Ala Val Ile Ala Glu Ser
565 570 575
Ala Gly Ile Asn Gly Ser Lys Ala Met Lys Leu Glu Asn Gly Thr Ile
580 585 590
Glu Gln Lys Leu Asn Leu Lys Pro Tyr Thr Asn Tyr Glu Phe Thr Ala
595 600 605
Tyr Ala Lys Gly Arg Ser Val Asn Leu Leu Ile Gln Asp Asn Thr Gln
610 615 620
Ala Ile Leu Ala Glu Gly Tyr Leu Asp Ser Thr Ile Tyr Lys Gln Met
625 630 635 640
Lys Phe Lys Phe Lys Thr Gly Met Asp Ser Ser Asn Phe Ile Val Arg
645 650 655
Ala Thr Ser Pro Gln Glu Val Leu Val Asp Asn Ile Lys Val Cys Glu
660 665 670
Thr Thr Gln Ala Asn Asn Leu Ile Glu Cys Gly Asp Phe Asn His Tyr
675 680 685
Glu Ile Asp Glu Glu Asp Phe Lys Ile Leu Leu Tyr Pro Asn Leu Trp
690 695 700
Glu Pro Tyr Asp Gly Gly Gly Ile Ala Ser Leu Gly Gly Leu Asn Asp
705 710 715 720
Ser Tyr Ser Leu Lys Ile Ile Asn Ser Gly Gly Ala Asn Gln Lys Val
725 730 735
Gln Leu Lys Ala Asn Thr Asn Tyr Ile Leu Thr Ala Tyr Val Lys Val
740 745 750
Asp His Pro Thr Thr Thr Ala Gln Ile Gly Cys Gly Ser Asn Gln Val
755 760 765
Ser Cys Asn Ser Thr Ser Tyr Thr Pro Leu Lys Leu Lys Phe Lys Thr
770 775 780
Gly Glu Asp Pro Ser Thr Thr Glu Ser Ser Val Tyr Cys Ser Asn Ser
785 790 795 800
Asn Asp Ser Gly Thr Val Trp Ala Asp Asn Phe Val Leu Tyr Glu Ala
805 810 815
Pro Asn Leu Ile Ile Asn Gly Asp Phe Glu Gln Phe Asp Gln Ser Ser
820 825 830
Trp Asn Phe Ser Pro Ser Glu Gly Gly Ser Ile Tyr Leu Gln Ser Gly
835 840 845
Leu Gly Met Ser Arg Ser Asn Ala Val Val Ile His Gly Lys Asn Gly
850 855 860
Lys Ile Ser Gln Asn Ile Pro Leu Lys Pro Phe Thr Lys Tyr Arg Leu
865 870 875 880
Thr Ala Tyr Val Arg Val Ser Lys Gly Ser Leu Ala Tyr Ile Gly Tyr
885 890 895
Gly Glu Tyr Asn Cys Ala Cys Ala Val Asp Asp Phe Arg Gln Ala His
900 905 910
Val Glu Phe Thr Thr Gly Ala Asn Pro Met Gln Ser Asp Asp Ala Ile
915 920 925
Tyr Leu Asp Ser Phe Ala Ser Gly Tyr Thr Val Ala Asp Asn Phe Glu
930 935 940
Leu Tyr Glu Leu Asp Gln Ile
945 950
<210> 49
<211> 949
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 49
Met Gln Met Val Thr Lys Ala Met Thr Thr Lys Leu Gly Ala Leu Pro
1 5 10 15
Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Asp Ser Ile
20 25 30
Thr Gly Val Met Ser Thr Ile Phe Gly Ile Asp Thr Gly Asp Ser Ser
35 40 45
Ile Asp Asp Val Leu Asn Asn Gln Glu Leu Leu Gln Glu Met Gln Ala
50 55 60
Gln Met Gln Gln Met Gln Asp Ser Ile Thr Asp Met Ala Asn Gln Gln
65 70 75 80
Ile Ile Ser Glu Gly Ile Glu Lys Gln Ile Leu Ala Phe Thr Thr Asp
85 90 95
Leu Ser Ala Ser Ile Thr Thr Glu Phe Lys Lys Ile Glu Gly Ile Leu
100 105 110
Asn Thr Tyr Leu Pro Ala Ile Ser Asn Met Leu Ser Asn Ile Tyr Glu
115 120 125
Gln Thr Ser Val Ile Asp Gln Lys Val Asp Lys Leu Leu Ala Leu Met
130 135 140
Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val Val Leu
145 150 155 160
Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu Val Tyr
165 170 175
Val Asn Lys Lys Phe Leu Ser Leu Thr Arg Gly Phe Leu Gln Gly Glu
180 185 190
Asp Val Ser Ile Asp Asn Met Gln Glu Ile Gln Glu Trp Ala Lys Ser
195 200 205
Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp Thr Leu
210 215 220
His Asn Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala Leu Lys
225 230 235 240
Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly Asp Phe
245 250 255
Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu Ala Thr
260 265 270
Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys Val Leu
275 280 285
Gly Leu Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg Ile Glu
290 295 300
Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn Tyr Ser
305 310 315 320
Asn Ala Leu Glu Ile Thr Gly Ile Tyr Pro Met Ser Leu Asp Trp Gly
325 330 335
Asn Cys Lys Ser Thr Asp Leu Gln Ala Asp Asp Gly Tyr Ala Leu Ile
340 345 350
Gly Leu Glu Phe Phe Met Asp Asn Gly Ile Tyr Lys Ala Lys Ala Tyr
355 360 365
Gln Gly Lys Ile Gly Lys Asn Phe Ser Val Ser Ala Asp Thr Val Thr
370 375 380
Glu Ile Ile Ser Asp Asp Leu Ser Thr Val Phe Asn Asp Met Thr Asn
385 390 395 400
Asp Asn Pro Glu Leu Tyr Val Val Tyr Pro Leu Ser Gly Glu Leu Thr
405 410 415
Gly Pro Pro Asn Thr Ile Ile Thr Arg Ile Gly Phe Gly Thr Lys Tyr
420 425 430
Asp Lys Thr Arg Glu Ser Ser Val Gln Ala Phe Ala Tyr Ile Asp Ala
435 440 445
Asp Phe Ser Pro Tyr Asp Tyr Ile Ser Gly Thr Ile Ser Lys Glu Gly
450 455 460
Thr Gln Thr Ile Ser Leu Glu Gly Asn Asp Pro Lys Asn Gln Cys Tyr
465 470 475 480
Ser Met Trp Pro Ile Gly Leu Ile Gly Asp Leu Tyr Met Thr Pro Leu
485 490 495
Lys Ser Leu Ser Leu Asn Val Asp Asp Ser Ser Ala Tyr Gly Thr Ile
500 505 510
Leu Asn Met Ser Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser Arg Glu
515 520 525
Tyr Asn Thr Asn Phe Ile Leu Phe Pro Tyr Thr Asn Asn Ser Ser Pro
530 535 540
Ile Val Glu Asn Leu Ile Gln Asn Gly Asp Phe Glu Asp Gly Asp Lys
545 550 555 560
Tyr Trp Glu Val Val Ser Gly Thr Ala Val Ile Ala Glu Ser Ala Gly
565 570 575
Ile Asn Gly Ser Lys Ala Met Lys Leu Glu Asn Gly Thr Ile Glu Gln
580 585 590
Lys Leu Asn Leu Lys Pro Tyr Thr Asn Tyr Glu Phe Thr Ala Tyr Ala
595 600 605
Lys Gly Arg Ser Val Asn Leu Leu Ile Gln Asp Asn Thr Gln Ala Ile
610 615 620
Leu Ala Glu Gly Tyr Leu Asp Ser Thr Ile Tyr Lys Gln Met Lys Phe
625 630 635 640
Lys Phe Lys Thr Gly Met Asp Ser Ser Asn Phe Ile Val Arg Ala Thr
645 650 655
Ser Pro Gln Glu Val Leu Val Asp Asn Ile Lys Val Cys Glu Thr Thr
660 665 670
Gln Ala Asn Asn Leu Ile Glu Cys Gly Asp Phe Asn His Tyr Glu Ile
675 680 685
Asp Glu Glu Asp Phe Lys Ile Leu Leu Tyr Pro Asn Leu Trp Glu Pro
690 695 700
Tyr Asp Gly Gly Gly Ile Ala Ser Leu Gly Gly Leu Asn Asp Ser Tyr
705 710 715 720
Ser Leu Lys Ile Ile Asn Ser Gly Gly Ala Asn Gln Lys Val Gln Leu
725 730 735
Lys Ala Asn Thr Asn Tyr Ile Leu Thr Ala Tyr Val Lys Val Asp His
740 745 750
Pro Thr Thr Thr Ala Gln Ile Gly Cys Gly Ser Asn Gln Val Ser Cys
755 760 765
Asn Ser Thr Ser Tyr Thr Pro Leu Lys Leu Lys Phe Lys Thr Gly Glu
770 775 780
Asp Pro Ser Thr Thr Glu Ser Ser Val Tyr Cys Ser Asn Ser Asn Asp
785 790 795 800
Ser Gly Thr Val Trp Ala Asp Asn Phe Val Leu Tyr Glu Ala Pro Asn
805 810 815
Leu Ile Ile Asn Gly Asp Phe Glu Gln Phe Asp Gln Ser Ser Trp Asn
820 825 830
Phe Ser Pro Ser Glu Gly Gly Ser Ile Tyr Leu Gln Ser Gly Leu Gly
835 840 845
Met Ser Arg Ser Asn Ala Val Val Ile His Gly Lys Asn Gly Lys Ile
850 855 860
Ser Gln Asn Ile Pro Leu Lys Pro Phe Thr Lys Tyr Arg Leu Thr Ala
865 870 875 880
Tyr Val Arg Val Ser Lys Gly Ser Leu Ala Tyr Ile Gly Tyr Gly Glu
885 890 895
Tyr Asn Cys Ala Cys Ala Val Asp Asp Phe Arg Gln Ala His Val Glu
900 905 910
Phe Thr Thr Gly Ala Asn Pro Met Gln Ser Asp Asp Ala Ile Tyr Leu
915 920 925
Asp Ser Phe Ala Ser Gly Tyr Thr Val Ala Asp Asn Phe Glu Leu Tyr
930 935 940
Glu Leu Asp Gln Ile
945
<210> 50
<211> 664
<212> БЕЛОК
<213> Bacillus sp.
<400> 50
Met Asn Thr Asn Asn Ser Asn Glu Leu Asn Asp Asp Ile Asn Thr Arg
1 5 10 15
Asn Gly Asp Pro Asn Ser Ile Val Thr Thr Asp Ser Gln Ile Ala Leu
20 25 30
Gln Lys Leu Glu Asn Lys Met Leu Asp Thr Phe Ile Glu Ser Ser Ala
35 40 45
Asn Val Thr Ile Asn Ser Arg Asp Ala Val Ser Phe Gly Ile Asn Phe
50 55 60
Ser Thr Lys Ile Leu Ser Leu Leu Gly Val Pro Phe Ser Ser Gln Ile
65 70 75 80
Gly Gln Leu Trp Asn Phe Thr Leu Asp Ala Leu Trp Pro Lys Gln Asp
85 90 95
Asp Thr Trp Asn Asn Leu Ile Asn Phe Val Glu Glu Leu Ile Asn Gln
100 105 110
Lys Val Glu Asn Ala Val Arg Lys Lys Ala Asn Ala Glu Leu Glu Gly
115 120 125
Leu Gly Asn Val Leu Glu Ile Tyr Tyr Asp Val Leu Ser Asp Trp Gln
130 135 140
Asp Glu Pro Asn Asn Glu Asn Arg Lys Glu His Val Arg Thr Gln Phe
145 150 155 160
Arg Ile Ala His Asn Leu Phe Glu Gln Gln Met Pro Ser Phe Ala Val
165 170 175
Glu Gly Phe Glu Val Pro Leu Leu Ala Val Tyr Ala Tyr Ala Ala Asn
180 185 190
Leu His Leu Leu Leu Leu Arg Asp Cys Ala Ile Tyr Gly Lys Asp Trp
195 200 205
Gly Phe Glu Pro Ile Glu Ile Asn Asn Asn Tyr Asn Arg Gln Leu Lys
210 215 220
Arg Thr Ile Lys Tyr Ser Asp His Cys Thr Asn Trp Tyr Asn Lys Gly
225 230 235 240
Leu Glu Asp Leu Arg Gly Pro Asn Gly Ser Asn Trp Ala Lys Tyr Asn
245 250 255
Lys Tyr Arg Arg Glu Met Thr Leu Asn Val Leu Asp Ile Cys Ser Leu
260 265 270
Phe Ser Asn Tyr Asp Tyr Gln Lys Tyr Pro Ile Glu Thr Arg Met Glu
275 280 285
Leu Thr Arg Asp Ile Tyr Thr Asp Ala Ile Pro Val Gly Thr Asp Met
290 295 300
Glu Gly Lys Ser Trp Ala Lys Lys Phe Pro Ser Phe Lys Thr Ile Glu
305 310 315 320
Glu Ile Ala Ile Arg Lys Pro His Thr Val Thr Trp Leu Asn Gln Leu
325 330 335
Thr Ile Tyr Thr Gly Arg Ser Ile Gly Trp Arg Gln Asn Phe Asp Tyr
340 345 350
Trp Met Gly His Lys Gln Thr Tyr Ser Glu Thr Ile Ala Gly Asn Asp
355 360 365
Ile Thr Gly Gln Leu Tyr Gly Arg Asp Ile Val Asn Asn His Leu Ile
370 375 380
Ser Asn Gly Tyr Asn Val Val Asn Lys Asp Ile Tyr Ser Ile Thr Ser
385 390 395 400
Glu Arg Tyr Val Asn Ala Tyr Pro Thr Gly Ile Met Gly Val Gly Ile
405 410 415
Ala Gly Ser Lys Phe Asp Phe Lys Asn Leu Ser Asn Gly Asn Ser Ser
420 425 430
Ser Asp Ser Tyr Gly Lys Asn Asp Tyr Pro Ser Val Ile Thr Gln Leu
435 440 445
Pro Gly Asp Asp Thr Asp Val Pro Thr Ala Glu Asp Tyr Ser His Arg
450 455 460
Leu Thr Tyr Leu Thr Gly Phe Glu Val Leu Lys Ser Gly Leu Ile Val
465 470 475 480
Cys Ala Ser Trp Thr Ser Thr Ser Val Asp Arg Asn Asn Lys Leu Glu
485 490 495
Asn Glu Ile Ile Thr Glu Ile Pro Gly Val Lys Gly His Ser Leu Asn
500 505 510
Arg Cys Asp Ile Val Lys Gly Ser Gly Ser Thr Gly Gly Asp Leu Leu
515 520 525
Lys Ser Thr Asn Ser Asn Ser Ser Phe Ser Leu Asn Val Arg Ser Leu
530 535 540
Asp Asp Gln Lys Tyr Arg Ile Arg Leu Arg Tyr Ala Ala Asp Thr Asn
545 550 555 560
Asn Thr Leu Gln Ile Lys Ser Ser Asp Ala Asp Ile Asn Ile Gly Val
565 570 575
Asp Ile Pro Lys Thr Gly Asn Ile Ser Asn Asp Asp Phe Ser Tyr Glu
580 585 590
Leu Phe Asn Tyr Val Lys Leu Pro Ile Glu Phe Lys Thr Asn Pro Ser
595 600 605
Asn Gly Asn Tyr Lys Asp Tyr Lys Phe Asp Phe Thr Phe Gly Arg Glu
610 615 620
Asp Met Tyr Ile Asp Arg Phe Glu Phe Ile Pro Ile Thr Asp Ser Leu
625 630 635 640
Ile Glu Tyr Glu Glu Lys Arg Lys Ile Ala Ile Asp Glu Leu Glu Lys
645 650 655
Gln Leu Ala Asp Leu Glu Asn Ser
660
<210> 51
<211> 636
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 51
Met Asn Thr Asn Asn Ser Asn Glu Leu Asn Asp Asp Ile Asn Thr Arg
1 5 10 15
Asn Gly Asp Pro Asn Ser Ile Val Thr Thr Asp Ser Gln Ile Ala Leu
20 25 30
Gln Lys Leu Glu Asn Lys Met Leu Asp Thr Phe Ile Glu Ser Ser Ala
35 40 45
Asn Val Thr Ile Asn Ser Arg Asp Ala Val Ser Phe Gly Ile Asn Phe
50 55 60
Ser Thr Lys Ile Leu Ser Leu Leu Gly Val Pro Phe Ser Ser Gln Ile
65 70 75 80
Gly Gln Leu Trp Asn Phe Thr Leu Asp Ala Leu Trp Pro Lys Gln Asp
85 90 95
Asp Thr Trp Asn Asn Leu Ile Asn Phe Val Glu Glu Leu Ile Asn Gln
100 105 110
Lys Val Glu Asn Ala Val Arg Lys Lys Ala Asn Ala Glu Leu Glu Gly
115 120 125
Leu Gly Asn Val Leu Glu Ile Tyr Tyr Asp Val Leu Ser Asp Trp Gln
130 135 140
Asp Glu Pro Asn Asn Glu Asn Arg Lys Glu His Val Arg Thr Gln Phe
145 150 155 160
Arg Ile Ala His Asn Leu Phe Glu Gln Gln Met Pro Ser Phe Ala Val
165 170 175
Glu Gly Phe Glu Val Pro Leu Leu Ala Val Tyr Ala Tyr Ala Ala Asn
180 185 190
Leu His Leu Leu Leu Leu Arg Asp Cys Ala Ile Tyr Gly Lys Asp Trp
195 200 205
Gly Phe Glu Pro Ile Glu Ile Asn Asn Asn Tyr Asn Arg Gln Leu Lys
210 215 220
Arg Thr Ile Lys Tyr Ser Asp His Cys Thr Asn Trp Tyr Asn Lys Gly
225 230 235 240
Leu Glu Asp Leu Arg Gly Pro Asn Gly Ser Asn Trp Ala Lys Tyr Asn
245 250 255
Lys Tyr Arg Arg Glu Met Thr Leu Asn Val Leu Asp Ile Cys Ser Leu
260 265 270
Phe Ser Asn Tyr Asp Tyr Gln Lys Tyr Pro Ile Glu Thr Arg Met Glu
275 280 285
Leu Thr Arg Asp Ile Tyr Thr Asp Ala Ile Pro Val Gly Thr Asp Met
290 295 300
Glu Gly Lys Ser Trp Ala Lys Lys Phe Pro Ser Phe Lys Thr Ile Glu
305 310 315 320
Glu Ile Ala Ile Arg Lys Pro His Thr Val Thr Trp Leu Asn Gln Leu
325 330 335
Thr Ile Tyr Thr Gly Arg Ser Ile Gly Trp Arg Gln Asn Phe Asp Tyr
340 345 350
Trp Met Gly His Lys Gln Thr Tyr Ser Glu Thr Ile Ala Gly Asn Asp
355 360 365
Ile Thr Gly Gln Leu Tyr Gly Arg Asp Ile Val Asn Asn His Leu Ile
370 375 380
Ser Asn Gly Tyr Asn Val Val Asn Lys Asp Ile Tyr Ser Ile Thr Ser
385 390 395 400
Glu Arg Tyr Val Asn Ala Tyr Pro Thr Gly Ile Met Gly Val Gly Ile
405 410 415
Ala Gly Ser Lys Phe Asp Phe Lys Asn Leu Ser Asn Gly Asn Ser Ser
420 425 430
Ser Asp Ser Tyr Gly Lys Asn Asp Tyr Pro Ser Val Ile Thr Gln Leu
435 440 445
Pro Gly Asp Asp Thr Asp Val Pro Thr Ala Glu Asp Tyr Ser His Arg
450 455 460
Leu Thr Tyr Leu Thr Gly Phe Glu Val Leu Lys Ser Gly Leu Ile Val
465 470 475 480
Cys Ala Ser Trp Thr Ser Thr Ser Val Asp Arg Asn Asn Lys Leu Glu
485 490 495
Asn Glu Ile Ile Thr Glu Ile Pro Gly Val Lys Gly His Ser Leu Asn
500 505 510
Arg Cys Asp Ile Val Lys Gly Ser Gly Ser Thr Gly Gly Asp Leu Leu
515 520 525
Lys Ser Thr Asn Ser Asn Ser Ser Phe Ser Leu Asn Val Arg Ser Leu
530 535 540
Asp Asp Gln Lys Tyr Arg Ile Arg Leu Arg Tyr Ala Ala Asp Thr Asn
545 550 555 560
Asn Thr Leu Gln Ile Lys Ser Ser Asp Ala Asp Ile Asn Ile Gly Val
565 570 575
Asp Ile Pro Lys Thr Gly Asn Ile Ser Asn Asp Asp Phe Ser Tyr Glu
580 585 590
Leu Phe Asn Tyr Val Lys Leu Pro Ile Glu Phe Lys Thr Asn Pro Ser
595 600 605
Asn Gly Asn Tyr Lys Asp Tyr Lys Phe Asp Phe Thr Phe Gly Arg Glu
610 615 620
Asp Met Tyr Ile Asp Arg Phe Glu Phe Ile Pro Ile
625 630 635
<210> 52
<211> 250
<212> БЕЛОК
<213> Bacillus sp.
<400> 52
Val Gln Lys Ser His Lys Lys Glu Gly Ile His Met Asn Tyr Pro Ile
1 5 10 15
Leu Asn Asn Thr Asn Ile Ser Ser Ser Leu Gln Gln Pro Arg Ile His
20 25 30
Arg Pro Ser Arg Thr Ile Ile Leu Thr Val Pro Ser Arg Asp Glu Ser
35 40 45
Asn Ile Arg Asn Ile Phe Gln Val Glu Pro Arg Tyr Ile Ala Arg Ala
50 55 60
Leu Thr Ala Ala Gln Ala Phe Gln Gly Ala Ile Gly Ser Asp Leu Asn
65 70 75 80
Phe Asn Tyr Asp Lys Ala Ile Gln Ile Ala Asn Asn Ile Pro Tyr Arg
85 90 95
Asp His Ser Asn Ile Ser Trp His Met Asn Arg Thr Val Ile Gln Glu
100 105 110
Thr Leu Gln Val Ser Pro Met Ile Ser Lys Ile Lys Asp Ile Leu Gly
115 120 125
Gly Phe Leu Tyr Phe Ile Asn Ile Ser Asn Ala Gly Phe Trp Asn Glu
130 135 140
Val Glu Ala Ala Ile Ile Asn Thr Phe Thr Asn Leu Tyr Thr Gln Thr
145 150 155 160
Asn Ser Ala Trp Ile Phe Trp Ser Ser Ser Thr Ser Thr Ser Thr Ser
165 170 175
Tyr Tyr Tyr Asn Ile Leu Phe Ala Ile Pro Arg Thr Ser Lys Ser Met
180 185 190
Val Val Ile Pro Ile Ala Phe Asp Ile Thr Val Arg Leu Glu Lys Gln
195 200 205
Lys Val Leu Phe Ile Thr Ile Lys Asp Arg Ala Glu Phe Arg Val Thr
210 215 220
Ile Lys Ala Ile Tyr Val His Val Leu Leu His Ser Leu Ser Asn Gln
225 230 235 240
Ser Ile Val Asp Val Phe Asn Pro Lys Ser
245 250
<210> 53
<211> 224
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 53
Val Gln Lys Ser His Lys Lys Glu Gly Ile His Met Asn Tyr Pro Ile
1 5 10 15
Leu Asn Asn Thr Asn Ile Ser Ser Ser Leu Gln Gln Pro Arg Ile His
20 25 30
Arg Pro Ser Arg Thr Ile Ile Leu Thr Val Pro Ser Arg Asp Glu Ser
35 40 45
Asn Ile Arg Asn Ile Phe Gln Val Glu Pro Arg Tyr Ile Ala Arg Ala
50 55 60
Leu Thr Ala Ala Gln Ala Phe Gln Gly Ala Ile Gly Ser Asp Leu Asn
65 70 75 80
Phe Asn Tyr Asp Lys Ala Ile Gln Ile Ala Asn Asn Ile Pro Tyr Arg
85 90 95
Asp His Ser Asn Ile Ser Trp His Met Asn Arg Thr Val Ile Gln Glu
100 105 110
Thr Leu Gln Val Ser Pro Met Ile Ser Lys Ile Lys Asp Ile Leu Gly
115 120 125
Gly Phe Leu Tyr Phe Ile Asn Ile Ser Asn Ala Gly Phe Trp Asn Glu
130 135 140
Val Glu Ala Ala Ile Ile Asn Thr Phe Thr Asn Leu Tyr Thr Gln Thr
145 150 155 160
Asn Ser Ala Trp Ile Phe Trp Ser Ser Ser Thr Ser Thr Ser Thr Ser
165 170 175
Tyr Tyr Tyr Asn Ile Leu Phe Ala Ile Pro Arg Thr Ser Lys Ser Met
180 185 190
Val Val Ile Pro Ile Ala Phe Asp Ile Thr Val Arg Leu Glu Lys Gln
195 200 205
Lys Val Leu Phe Ile Thr Ile Lys Asp Arg Ala Glu Phe Arg Val Thr
210 215 220
<210> 54
<211> 406
<212> БЕЛОК
<213> Bacillus sp.
<400> 54
Met Thr Asp Asn Leu Asn Gly Asp Pro Asn Glu Asn Thr Val Thr Asn
1 5 10 15
Asp Asp Ser Leu Ala Asn Asp Glu Arg Asn Glu Ser Leu Pro Glu Glu
20 25 30
Ser Arg Met Phe Ala Tyr Tyr Gln Pro Val Ser Thr Asn Asn Lys Val
35 40 45
Trp Asp Ala Ser Glu Gly Gly Gly Gly Ile Phe Phe Gly Cys Glu Gly
50 55 60
Ser Phe Leu Pro Asn Ala Met Tyr Asn His Leu Tyr His Gly Gly Leu
65 70 75 80
Asn Gln Gln Phe Ile Trp Phe Gln Leu Asp Asp Gly Arg Phe Ala Ile
85 90 95
Ala Asn Lys Gln Asn Gly Tyr Val Leu Gln Lys Tyr Asn Ala Ala Asn
100 105 110
Lys Ile Phe Glu Ile Val Asn Thr Pro Trp Glu Asn Asn Tyr Ala Gln
115 120 125
Leu Trp Arg Lys Glu Ser Thr Ser Ala Thr Asn Phe Arg Leu Val Asn
130 135 140
Glu Ala Glu Ala Leu Glu Val Cys Gly Gly Asn Thr Ser Ala Gln Ala
145 150 155 160
Lys Met Ser Phe Ile Pro Asn Arg Pro Asp Asn Ala Leu Leu Phe Arg
165 170 175
Arg Arg Ala Ile Ala Gln Asn Asn Val Val Pro Ala Leu Pro Ser Lys
180 185 190
Gln Pro Leu Pro Ala Leu Pro Ile Leu Ser Asn Leu Asn Asp Pro Ala
195 200 205
Pro His Glu Thr Ala Arg Ala Ile Thr Gly Ser Ser Leu Val Pro Cys
210 215 220
Ile Met Val Ser Asp Tyr Ile Pro Val Ala Gln Arg Ile Lys Asp Ser
225 230 235 240
Pro Tyr Tyr Ile Leu Val Lys Lys Gln Phe Trp Lys Arg Leu Trp His
245 250 255
Asp Thr Phe Ile Pro Gly Glu Tyr Arg Glu Phe Gln Glu Thr Thr Gly
260 265 270
Met Ser Lys Thr Gln Gln Glu Ser Met Thr Gln Thr Thr Asn Ile Ser
275 280 285
Leu Gln Ala Asp Phe Gly Phe Ala Phe Lys Ala Leu Thr Leu Gly Leu
290 295 300
Gly Val Ser Ile Ser Arg Ser Ile Glu Ile Ser Arg Ser Thr Thr Asp
305 310 315 320
Thr Val Met Ser Gln Tyr Thr Asp Thr Gln Gly Tyr Lys Asn Glu Asn
325 330 335
Ser Ile Lys Ile Ser Trp Ala Lys Tyr Ala Leu Ala Thr Glu Phe Ile
340 345 350
Leu Met Arg Lys Asp Gly Thr Thr Ala Gly Ser Trp Glu Ala Val Asp
355 360 365
Lys Lys Val Thr Arg Ser Ile Ser Tyr Pro Pro Thr Gln Thr Leu Ser
370 375 380
Trp Thr Lys Ser Leu Glu Asp Gln Glu Asp Asn Thr Val Phe Glu Pro
385 390 395 400
Thr Thr Ile Lys Ser Glu
405
<210> 55
<211> 353
<212> БЕЛОК
<213> Bacillus sp.
<400> 55
Met Val Lys Arg Asp Ile Ser Pro Arg Gly Leu Gly Gly Ser Lys Glu
1 5 10 15
Ser Pro Phe Leu Leu Ser Lys Ala Glu Trp Ile Met Ile Gln Lys Tyr
20 25 30
Thr Gly Asp Gly Val Tyr Val Pro Val Asn Glu Ala Glu Met Arg Lys
35 40 45
Thr Leu Ala Leu Ser Ser Ser Asp Glu Ile Pro Asp Phe Ser Glu Leu
50 55 60
Tyr Ser Ile Tyr Ser Asn Val Lys Ser His Cys Gln Asn Trp Thr Asp
65 70 75 80
Asn Thr Tyr Arg Glu Val Leu Gly Val Ala Asn Glu Ile Val Asn Tyr
85 90 95
Ser Arg Arg Ala Thr Val Tyr Tyr Lys Pro Leu Leu Asp Tyr Leu Pro
100 105 110
Ala Ile Leu Asp Gly Asp Thr Lys Ser Leu Glu Met Phe Gln Lys Ile
115 120 125
Cys Lys Lys Leu Ala Lys Glu Ala Ala Glu Phe Arg Asp His Ala Gly
130 135 140
Ala Leu Ala Thr Met Ile Gly Val Phe Ala Lys Asp Thr Ala Asn Asp
145 150 155 160
Tyr Arg Asp Leu Thr Glu Ile Lys Val Lys Tyr Val Glu Ile Tyr Gly
165 170 175
Glu His Ser Asp Glu Val Thr Glu Leu Arg Glu Ser Ile Glu Glu Leu
180 185 190
Arg Glu Glu Leu Glu Asp Ala Met Asp Asn Tyr Glu Asp Tyr Lys Ser
195 200 205
Gln Ser Trp Leu Ser Leu Leu Leu Gly Pro Leu Trp Gly Leu Val Leu
210 215 220
Lys Gly Val Leu Asp Ser Thr Glu Gly Lys Val Leu Glu Ala Lys Val
225 230 235 240
Lys Ala Leu Glu Glu Gln Ile Glu Gln Ala Glu Lys Ile Ile Lys Arg
245 250 255
Asn Val Tyr Leu Met Ser Leu Leu Asp Lys Thr Asp Ser Gly Ile Asn
260 265 270
Lys Ile Gln Gln Gln Met Gly Asp Ala Leu Pro Ile Ile Gln Lys Ile
275 280 285
His Gly Ile Trp Asn Ser Leu His Asn Asp Leu Asp Glu Leu Ser Lys
290 295 300
Ile Val Met Lys Asp Ile His Asp Asp Pro Glu Phe Ala Asp Leu Gly
305 310 315 320
Ile Glu Leu Ala Ile Met Gln Trp Glu Val Val Gly Gln Glu Ala Asp
325 330 335
Asp Phe Arg Val Asn Ala Asp Val Glu Phe Ile Val Glu Gln Tyr Thr
340 345 350
Ala
<210> 56
<211> 572
<212> БЕЛОК
<213> Streptomyces sp.
<400> 56
Val Arg Thr Leu Leu Ala Gln Leu Leu Glu Pro Gly Gly Arg Val Gly
1 5 10 15
Leu Glu Leu Gly Gln Leu Gly Ala Arg Asp Asp Thr Leu Leu Glu Leu
20 25 30
Cys Gly Gly Gly Leu Glu Val His Thr Gln Leu Leu Arg Arg Gly Arg
35 40 45
Gly Leu His Gly Leu Gly Val Leu Val Arg Arg His Gly Val Lys Gly
50 55 60
Ile Glu His Ala Gly Glu Leu Arg Pro Cys Arg Leu Pro Val Thr Trp
65 70 75 80
Arg Gly Asp Arg Leu Gly Arg Phe Pro Arg Gly Tyr Pro His Phe Pro
85 90 95
Leu Leu Leu Pro Ala Arg Leu Arg Ala Gly Gly Asp Pro Arg Glu Val
100 105 110
Gly Ala Asp Leu Glu Arg Ala Pro Ala Arg Arg Ala Pro Asp Ala Ala
115 120 125
Arg Arg Asp Thr Gly Arg Gly Leu Arg Arg Ala Pro Gly Gly Arg Arg
130 135 140
Gly Arg Gly Ser Arg Ala Ala Ala Arg Arg Pro Gly Arg Pro Gln Arg
145 150 155 160
Asp Pro Ala Leu His Gly Asp Asp Arg Arg Arg His Pro Gln Gly Pro
165 170 175
Pro Val Arg Gly Gly Gly Gly Gly Val Gly Arg Gly Pro Arg Arg Gly
180 185 190
Ala Arg Pro Ala Pro Ala Arg Arg His Pro Arg Leu Gly Ala Ala Ala
195 200 205
Gly Pro Pro Gly Asp Gly Ala Ala Arg Asp Asp Gly Arg Arg Asp Arg
210 215 220
Ala Gly Arg Arg Gly Arg Arg Arg Pro Arg Arg Pro Pro Val Ala Gly
225 230 235 240
Pro Ser Ala Pro Pro Gln Gly Pro His Leu Pro Asp Pro Arg Arg Arg
245 250 255
Pro Ala Val Ala Gly Gly Pro Val Val Ala Gly Arg Gly Arg Gly Ala
260 265 270
Arg Pro Gly Gly Gly Val His Arg His Arg Ala Arg Pro His Arg Glu
275 280 285
Gln His Pro Gly Pro Pro Gly Arg Gly Glu Glu Gly Pro Glu Ala Ala
290 295 300
Gln Gly Ala Ala Ala Arg Glu Ala Gly Ala Leu Arg Glu Ala Gly Arg
305 310 315 320
Arg Leu Pro His Leu Arg Pro Ser Pro Gly Val Pro Gln Gly Arg Arg
325 330 335
Arg Leu Arg Pro Glu Arg Glu Ala Ala Pro Arg Pro Gly Leu Ser Arg
340 345 350
Pro His Gly Gly Arg Pro Ala Pro Gly Arg Arg Arg Leu Gly Ile Leu
355 360 365
Thr Pro Pro Asp His Arg Asp His Ala Gly Gly Ala Leu Ala Ala Ser
370 375 380
Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu Ala Gln Ala Arg
385 390 395 400
Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro Ala Thr Val Asn
405 410 415
Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ser Ala Val Pro Asp Ser
420 425 430
Asp Val Val Arg Met Val Arg Gly Trp Gly Leu Gln Glu His Ala Pro
435 440 445
Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val Arg Gln Ala Leu
450 455 460
Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr Val Glu Arg Glu
465 470 475 480
Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu Gly Gln Pro Gly
485 490 495
Leu Ser Phe Tyr Glu Glu Ser Gly Asp Arg Thr Arg Phe Tyr Arg Asp
500 505 510
Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp His Met Tyr Ala
515 520 525
Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro Lys Ala Glu Val
530 535 540
Leu Ala Leu Asp Leu Thr Asp Ile Val Pro Phe Thr Val Arg Leu Asn
545 550 555 560
Ala Ile Val Val Arg Gln Ala Leu Ser Val Ser Val
565 570
<210> 57
<211> 1273
<212> БЕЛОК
<213> Bacillus sp.
<400> 57
Met Asn Gln Tyr Tyr Arg Asn Asn Glu Met Glu Ile Leu Asp Pro Gly
1 5 10 15
Val Arg Arg Ala Lys Tyr Pro Tyr Ala Gln Asn Thr Arg Thr Gly Leu
20 25 30
Gln Ser Met Asn Tyr Lys Glu Trp Leu Thr Leu Cys Glu Gly Asn Asp
35 40 45
Gly Leu Ser Pro Ser Pro Phe Val Ala Pro Gln Asp Ile Val Ser Val
50 55 60
Thr Leu Asp Ile Thr Gly Thr Leu Leu Gly Leu Ile Pro Val Val Gly
65 70 75 80
Gly Pro Leu Lys Ala Gly Val Ser Ile Leu Asn Ser Ile Ile Lys Leu
85 90 95
Leu Trp Pro Lys Pro Thr Pro Glu Glu Pro Asp Pro Asn Glu Ile Trp
100 105 110
Ile Gln Leu Met Glu Lys Val Glu Gln Leu Ile Asp Gln Ala Ile Ser
115 120 125
Lys Glu Ala Phe Asn Ala Ala Ile Gly Arg Leu Thr Gly Leu Asn Arg
130 135 140
Ala Leu Asn Leu Tyr Gln Val Ala Phe Glu Asp Trp Ala Lys Asp Gln
145 150 155 160
Thr Asp Glu Glu Leu Lys Glu Asp Leu Arg Asn Arg Phe Thr Asn Ala
165 170 175
Leu Phe Glu Met Val Ser Thr Ile Glu Thr Phe Arg Tyr Lys Asp Gln
180 185 190
Glu Val Asn Leu Leu Thr Val Phe Ala Gln Ala Ala Asp Leu His Leu
195 200 205
Leu Leu Leu Arg Glu Gly Ile Met Tyr Gly Val Gln Trp Gly Phe Asp
210 215 220
Glu Arg Thr Val Gln Ser Phe Tyr Thr Asn Phe Gln Asn Glu Gly Leu
225 230 235 240
His Asp Leu Leu Pro Gln Tyr Thr Asp Tyr Cys Ile Lys Trp Tyr Asn
245 250 255
Glu Gly Leu Arg Ile Thr Tyr Thr Leu Lys Pro Asp Leu Ser Asp Thr
260 265 270
Glu Arg Tyr Pro Trp Ala Ala Asp Lys Ala Ser Trp Asn Leu Gln Val
275 280 285
Leu Glu Glu Leu Glu Gly Trp Asn Leu Phe Asn Asp Trp Arg Arg Asp
290 295 300
Val Thr Ile Met Ala Leu Asp Leu Val Ser Leu Trp Pro Thr Tyr Asp
305 310 315 320
Leu Tyr Tyr Tyr Asn Asn Leu Glu Tyr Gly Val Thr Thr Glu Leu Thr
325 330 335
Arg Pro Val Tyr Ser Gln Ala Ala Gly Thr Ser Trp Gly Ser Ile Leu
340 345 350
Pro Arg Asp Arg Tyr Glu Gln Gly Ile Val Arg Ser Pro His Leu Val
355 360 365
Thr Trp Leu Asn Ser Ile Asp Phe His Leu Asn Pro Gly Pro Phe Asn
370 375 380
Ala Ser Asn Ser Tyr Lys His Tyr Thr Gly Leu Lys Met Lys Tyr Tyr
385 390 395 400
Tyr Ser Gly Arg Asp Tyr Thr Thr Tyr Glu Ser Pro Leu Ile Gly Thr
405 410 415
Thr Ser Tyr Ser Lys Lys Thr Ile Asn Val Glu Ala Lys Tyr Ser Asn
420 425 430
Asn Asp Ile Phe Gln Ala Asp Ile Lys His Asn Asp Ala Val Tyr Thr
435 440 445
Met Asp Phe Phe Lys Asn Asn Gly Asn Ile Glu Lys Val Gly Tyr Pro
450 455 460
Arg Asp Pro Asn Tyr Ser Thr Phe Ser Trp Pro Pro Glu Thr Asp Ile
465 470 475 480
Ser Asn Tyr Asp His Gln Met Thr Trp Ile Gly Gly Pro Ala Glu Lys
485 490 495
Asn Thr Ile Pro Ala Phe Gly Ile Glu Trp Met His Glu Ser Cys Ser
500 505 510
Pro Phe Asn Ala Ile Ala Asp Asp Thr Val Glu Asn Pro Ile Ile Thr
515 520 525
Gln Ile Pro Ala Val Lys Gly Thr Asn Leu Ser Gly Asn Ala Thr Val
530 535 540
Ile Lys Asn Pro Gly His Thr Gly Gly Asp Leu Val Gln Leu Pro Met
545 550 555 560
Asn Ser Ala Ser Gln Ala Gln Leu Leu Val Asn Met Glu Ser Pro Tyr
565 570 575
Ser Lys Val Gly Lys Thr Tyr His Ile Arg Ile Arg Tyr Ala Ser Ser
580 585 590
Thr Asn Ala Asn Leu Gln Val Asp His Cys Gly Phe Asn Gly Gly Ser
595 600 605
Cys Ser Ser Ser Val Thr Ile Pro Ile Lys Pro Thr Tyr Ser Asn Asn
610 615 620
Glu Leu Thr Tyr Asn Ala Phe Gln Tyr Val Asp Val Phe Thr Ile Thr
625 630 635 640
Leu Gln Asn Ala Asp Phe Ser Ile Ala Phe Ser Asn Lys Gly Gly Gly
645 650 655
Pro Ile Ile Leu Asp Lys Ile Glu Phe Ile Pro Ile Glu Glu Ser Val
660 665 670
Glu Glu Tyr Glu Ala Asn Gln Thr Phe Lys Lys Ala Arg Lys Ala Val
675 680 685
Asn Ala Leu Phe Ala Asn Asp Glu Lys Ile Ala Leu Arg Leu Asn Ile
690 695 700
Thr Asp Tyr Asp Val Asn Gln Val Ala Asn Ile Val Asp Cys Ile Ser
705 710 715 720
Asp Asp Met Phe Pro Lys Glu Lys Met Val Leu Leu Asp Gln Val Lys
725 730 735
Gln Ala Lys Arg Leu Ser Gln Ala Arg Asn Leu Leu Asn Tyr Gly Asp
740 745 750
Phe Glu Ser Pro Asp Trp Ser Asp Glu Asn Gly Trp Lys Val Ser Asn
755 760 765
Asn Val Met Val Gln Thr Asp Arg Pro Ile Ser Arg Gly Arg Tyr Leu
770 775 780
Asn Met Pro Gly Ala Arg Val Ile Glu Phe Ser Asp Thr Ile Tyr Pro
785 790 795 800
Thr Tyr Val Tyr Gln Lys Ile Asp Glu Ser Lys Leu Lys Pro Tyr Thr
805 810 815
Arg Tyr Leu Val Arg Gly Phe Val Lys Ser Ser Lys Lys Leu Asp Leu
820 825 830
Phe Val Thr Arg Tyr Gly Lys Asp Ile Arg Thr Glu Met Asn Val Pro
835 840 845
Ser Tyr Gln Val Ile Asn Asn Gln Val Val Arg Met Leu Asp Ser Cys
850 855 860
Asp Thr Lys Gln Val Ser His Tyr Thr Ile Ser Pro Thr Met Pro Met
865 870 875 880
Asp Pro Cys Gln Asn Ala Tyr Thr Thr Asn Asp Ser Ile Val Met Asn
885 890 895
Pro Ser Thr Asn Gly Met Cys Glu Asp Lys His His Phe Leu Phe His
900 905 910
Ile Asp Val Gly Glu Leu Asp Met Arg Ala Asn Pro Gly Ile Glu Val
915 920 925
Gly Phe Lys Ile Ser Ser Pro Glu Gly Met Ala Lys Leu Asp Asn Leu
930 935 940
Glu Val Ile Glu Ala Gln Pro Leu Thr Gly Glu Ala Leu Ala Arg Val
945 950 955 960
Lys Lys Arg Glu Gln Lys Trp Lys Lys Glu Arg Glu His Gln Cys Val
965 970 975
Lys Thr Glu Lys Ala Val Thr Ser Ala Ala Gln Ala Val Asp Ser Leu
980 985 990
Phe Thr Ser Pro Gln Lys Asn Lys Leu Lys Glu Thr Val Asn Met Gln
995 1000 1005
Ser Ile Glu Asn Ala Glu Ala Lys Val Asn Ala Ile Pro Tyr Val
1010 1015 1020
Gln Asn Pro Glu Phe Lys Asp Leu Pro Gly Met Asn Thr Leu Ile
1025 1030 1035
Phe Gln Lys Leu Gln Ser Asn Ile Met Thr Ala Leu Asn Leu Tyr
1040 1045 1050
Gly Arg Arg Asn Val Ile Arg Asn Gly Asp Phe Ser Arg Gly Leu
1055 1060 1065
Thr Asn Trp Leu Val Thr Asp Gly Val Asp Val Glu Glu Arg Asp
1070 1075 1080
Gly Lys Phe Ser Val Leu Val Ile Ser Gln Trp Asp Ala Asn Val
1085 1090 1095
Ser Gln Asp Val Cys Val Gln Pro Glu His Gly Tyr Val Leu Arg
1100 1105 1110
Val Thr Ala Arg Lys Glu Gly Ala Gly Lys Gly Tyr Val Thr Ile
1115 1120 1125
Ser Asp Cys Thr Pro Glu Asn Thr Glu Thr Ile Thr Phe Thr Ser
1130 1135 1140
Asp Glu Met Ile Met Ala Glu Arg Pro Pro Val Thr Ile Gln Thr
1145 1150 1155
Ser Ser Val Cys Asp Ile Thr Asp Asn Tyr Gly Thr Gly Ser Ser
1160 1165 1170
Pro Tyr Asn Asn Thr Val Glu Lys Ser Tyr Leu Glu Gly Leu Asp
1175 1180 1185
Ile Ile Pro Cys Ser Cys Gly Ser Tyr Gly Arg Glu Ala Cys Ser
1190 1195 1200
Cys Gly Ser Asn Tyr Met Arg Asn Ser Ser Ser Glu Arg Asn Ile
1205 1210 1215
Lys Gln Ser Tyr Thr Ser Thr Gln Arg Ile Pro Asn Arg Asn Ser
1220 1225 1230
Ser Val Tyr Val Thr Lys Met Ile Glu Met Phe Pro Glu Thr Asn
1235 1240 1245
Arg Ile Arg Ile Glu Ile Gly Glu Thr Gln Gly Thr Phe Gln Val
1250 1255 1260
Asp Ser Ile Glu Leu Ile Cys Met Glu Asp
1265 1270
<210> 58
<211> 668
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 58
Met Asn Gln Tyr Tyr Arg Asn Asn Glu Met Glu Ile Leu Asp Pro Gly
1 5 10 15
Val Arg Arg Ala Lys Tyr Pro Tyr Ala Gln Asn Thr Arg Thr Gly Leu
20 25 30
Gln Ser Met Asn Tyr Lys Glu Trp Leu Thr Leu Cys Glu Gly Asn Asp
35 40 45
Gly Leu Ser Pro Ser Pro Phe Val Ala Pro Gln Asp Ile Val Ser Val
50 55 60
Thr Leu Asp Ile Thr Gly Thr Leu Leu Gly Leu Ile Pro Val Val Gly
65 70 75 80
Gly Pro Leu Lys Ala Gly Val Ser Ile Leu Asn Ser Ile Ile Lys Leu
85 90 95
Leu Trp Pro Lys Pro Thr Pro Glu Glu Pro Asp Pro Asn Glu Ile Trp
100 105 110
Ile Gln Leu Met Glu Lys Val Glu Gln Leu Ile Asp Gln Ala Ile Ser
115 120 125
Lys Glu Ala Phe Asn Ala Ala Ile Gly Arg Leu Thr Gly Leu Asn Arg
130 135 140
Ala Leu Asn Leu Tyr Gln Val Ala Phe Glu Asp Trp Ala Lys Asp Gln
145 150 155 160
Thr Asp Glu Glu Leu Lys Glu Asp Leu Arg Asn Arg Phe Thr Asn Ala
165 170 175
Leu Phe Glu Met Val Ser Thr Ile Glu Thr Phe Arg Tyr Lys Asp Gln
180 185 190
Glu Val Asn Leu Leu Thr Val Phe Ala Gln Ala Ala Asp Leu His Leu
195 200 205
Leu Leu Leu Arg Glu Gly Ile Met Tyr Gly Val Gln Trp Gly Phe Asp
210 215 220
Glu Arg Thr Val Gln Ser Phe Tyr Thr Asn Phe Gln Asn Glu Gly Leu
225 230 235 240
His Asp Leu Leu Pro Gln Tyr Thr Asp Tyr Cys Ile Lys Trp Tyr Asn
245 250 255
Glu Gly Leu Arg Ile Thr Tyr Thr Leu Lys Pro Asp Leu Ser Asp Thr
260 265 270
Glu Arg Tyr Pro Trp Ala Ala Asp Lys Ala Ser Trp Asn Leu Gln Val
275 280 285
Leu Glu Glu Leu Glu Gly Trp Asn Leu Phe Asn Asp Trp Arg Arg Asp
290 295 300
Val Thr Ile Met Ala Leu Asp Leu Val Ser Leu Trp Pro Thr Tyr Asp
305 310 315 320
Leu Tyr Tyr Tyr Asn Asn Leu Glu Tyr Gly Val Thr Thr Glu Leu Thr
325 330 335
Arg Pro Val Tyr Ser Gln Ala Ala Gly Thr Ser Trp Gly Ser Ile Leu
340 345 350
Pro Arg Asp Arg Tyr Glu Gln Gly Ile Val Arg Ser Pro His Leu Val
355 360 365
Thr Trp Leu Asn Ser Ile Asp Phe His Leu Asn Pro Gly Pro Phe Asn
370 375 380
Ala Ser Asn Ser Tyr Lys His Tyr Thr Gly Leu Lys Met Lys Tyr Tyr
385 390 395 400
Tyr Ser Gly Arg Asp Tyr Thr Thr Tyr Glu Ser Pro Leu Ile Gly Thr
405 410 415
Thr Ser Tyr Ser Lys Lys Thr Ile Asn Val Glu Ala Lys Tyr Ser Asn
420 425 430
Asn Asp Ile Phe Gln Ala Asp Ile Lys His Asn Asp Ala Val Tyr Thr
435 440 445
Met Asp Phe Phe Lys Asn Asn Gly Asn Ile Glu Lys Val Gly Tyr Pro
450 455 460
Arg Asp Pro Asn Tyr Ser Thr Phe Ser Trp Pro Pro Glu Thr Asp Ile
465 470 475 480
Ser Asn Tyr Asp His Gln Met Thr Trp Ile Gly Gly Pro Ala Glu Lys
485 490 495
Asn Thr Ile Pro Ala Phe Gly Ile Glu Trp Met His Glu Ser Cys Ser
500 505 510
Pro Phe Asn Ala Ile Ala Asp Asp Thr Val Glu Asn Pro Ile Ile Thr
515 520 525
Gln Ile Pro Ala Val Lys Gly Thr Asn Leu Ser Gly Asn Ala Thr Val
530 535 540
Ile Lys Asn Pro Gly His Thr Gly Gly Asp Leu Val Gln Leu Pro Met
545 550 555 560
Asn Ser Ala Ser Gln Ala Gln Leu Leu Val Asn Met Glu Ser Pro Tyr
565 570 575
Ser Lys Val Gly Lys Thr Tyr His Ile Arg Ile Arg Tyr Ala Ser Ser
580 585 590
Thr Asn Ala Asn Leu Gln Val Asp His Cys Gly Phe Asn Gly Gly Ser
595 600 605
Cys Ser Ser Ser Val Thr Ile Pro Ile Lys Pro Thr Tyr Ser Asn Asn
610 615 620
Glu Leu Thr Tyr Asn Ala Phe Gln Tyr Val Asp Val Phe Thr Ile Thr
625 630 635 640
Leu Gln Asn Ala Asp Phe Ser Ile Ala Phe Ser Asn Lys Gly Gly Gly
645 650 655
Pro Ile Ile Leu Asp Lys Ile Glu Phe Ile Pro Ile
660 665
<210> 59
<211> 627
<212> БЕЛОК
<213> Bacillus sp.
<400> 59
Met Gly Lys Thr Ser Gly Glu Val Lys Met Leu Arg Thr Arg Gln Tyr
1 5 10 15
Arg Asn Ala Ser Glu Met Val Tyr Asp Gln Pro Leu Pro Phe Gly Gly
20 25 30
Gly Val Lys Ile Ala Gly Lys Ile Gly Leu Gly Val Val Lys Thr Val
35 40 45
Ile Thr Ser Ile Ile Arg Arg Gly Arg Asp Asn Glu Ile Ala Gly Arg
50 55 60
Ile Leu Ser Asp Val Tyr Asn Ile Leu Trp Ser Asn His Lys Gly Tyr
65 70 75 80
Trp Asn Glu Met Ile Glu Ala Val Glu Thr Leu Ile Gln His Asp Ile
85 90 95
Ser Glu Asn Ile Arg Asn Asn Ala Leu Ala Val Leu Thr Asp Val Arg
100 105 110
Asn Pro Leu Leu Gln Tyr Gln Gln Ala Val Asp Asp Trp Gln Arg Asn
115 120 125
Arg Thr Asp Ser Lys Leu Gln Glu Gln Val Arg Asn Gln Leu Ile Ala
130 135 140
Thr Asn Thr Phe Ile Glu Phe Ala Met Pro Ser Phe Thr Val Pro His
145 150 155 160
Tyr Glu Val Ile Leu Leu Pro Ile Phe Ala Gln Val Ala Asn Leu His
165 170 175
Leu Leu Leu Leu Arg Asp Ile Glu Ile Phe Gly Leu Glu Trp Gly Met
180 185 190
Ser Lys Ala Glu Ile Asp Asp Tyr Tyr Phe Ala Asn Ser Gly Leu Thr
195 200 205
Gly Leu Thr Gln Lys Tyr Thr Asn His Ile Val Lys Trp Tyr Ser Glu
210 215 220
Gly Leu Cys Ile Ala Thr Asn Ile Asp Leu Gly Gln Cys Pro Glu Phe
225 230 235 240
Tyr Gln Leu Asp Lys Trp Asn Ala Met Asn Asp Phe Arg Arg Glu Met
245 250 255
Thr Phe Met Val Leu Asp Ile Ile Ala Leu Trp Pro Thr Tyr Asp Pro
260 265 270
Ile Arg Tyr Pro Leu Gly Ile Lys Thr Glu Leu Thr Arg Glu Val Phe
275 280 285
Thr Pro Leu Leu Gly Ile Asn Pro Asn Ser Ser Ser Leu Ile His Thr
290 295 300
Met Glu Glu Ile Glu Asp Lys Leu Thr Phe Leu Ser Pro Phe Leu Ser
305 310 315 320
Trp Ile Ser Phe Glu Gln Leu Val Lys Gln Gly Asp Gly Ile Ser Thr
325 330 335
Phe Thr Asp Trp Gly Asn Phe Thr Leu Ser Asn Thr Met Leu Pro Ile
340 345 350
Ser Tyr Ile Leu Gly Gly Ala Gly Ser Gly Thr Gly Asp Ser Ile Asn
355 360 365
Ile Pro Ile Lys Ser Glu Asn Tyr Asp Val Tyr Lys Val Arg Val Gly
370 375 380
Thr Asp Tyr Ser His Pro Ser Asn Val Pro Ile Arg Lys Leu Glu Tyr
385 390 395 400
Tyr Cys Thr Asn Gly Thr Met Glu Asn Val Ile Thr Val Gly Thr Gly
405 410 415
Thr Thr Asn Ala Leu Phe Glu Leu Pro Asn Asn Gly Tyr Ile Asp Tyr
420 425 430
Ser His Arg Val Ser Arg Leu Ser Cys Ser Asn Val Glu Val Tyr Glu
435 440 445
Trp Glu Gly Gly Pro Lys Tyr Ala Leu Lys Asn Ile Ala Tyr Gly Trp
450 455 460
Thr His Ile Ser Val Asp Ser Lys Asn Thr Leu Ser Ala Asn Ala Ile
465 470 475 480
Thr Gln Ile Pro Ala Arg Lys Gly Tyr Ser Ser Ser Glu Ser Asn Leu
485 490 495
Ser Ile Ala Gly Pro Tyr Phe Thr Gly Gly Asp Leu Ile Ser Leu Pro
500 505 510
Pro Asn Gly Ala Gln Leu Gln Met Arg Val Thr Pro Pro Val Ser Ser
515 520 525
Ser Thr Thr Asn Tyr Cys Val Arg Leu Arg Tyr Ala Ser Ser Gly Asn
530 535 540
Thr Asn Ile Tyr Val Glu Arg Val Leu Ser Ser Gly Asp Thr Tyr Gly
545 550 555 560
Glu Thr His Asn Val Pro Ala Thr Tyr Ser Gly Gly Ser Leu Ser Tyr
565 570 575
Ser Ser Phe Ala Tyr Val Val Asn Leu Thr Ala Ile Phe Glu Gly Phe
580 585 590
Asn Val Glu Ile Lys Ile Lys Asn Ile Gly Ser Ser Gln Ile Ile Leu
595 600 605
Asp Lys Ile Glu Phe Leu Pro Ile Lys Glu Ser Leu Glu Gly Asn Lys
610 615 620
Lys Leu Thr
625
<210> 60
<211> 618
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 60
Met Leu Arg Thr Arg Gln Tyr Arg Asn Ala Ser Glu Met Val Tyr Asp
1 5 10 15
Gln Pro Leu Pro Phe Gly Gly Gly Val Lys Ile Ala Gly Lys Ile Gly
20 25 30
Leu Gly Val Val Lys Thr Val Ile Thr Ser Ile Ile Arg Arg Gly Arg
35 40 45
Asp Asn Glu Ile Ala Gly Arg Ile Leu Ser Asp Val Tyr Asn Ile Leu
50 55 60
Trp Ser Asn His Lys Gly Tyr Trp Asn Glu Met Ile Glu Ala Val Glu
65 70 75 80
Thr Leu Ile Gln His Asp Ile Ser Glu Asn Ile Arg Asn Asn Ala Leu
85 90 95
Ala Val Leu Thr Asp Val Arg Asn Pro Leu Leu Gln Tyr Gln Gln Ala
100 105 110
Val Asp Asp Trp Gln Arg Asn Arg Thr Asp Ser Lys Leu Gln Glu Gln
115 120 125
Val Arg Asn Gln Leu Ile Ala Thr Asn Thr Phe Ile Glu Phe Ala Met
130 135 140
Pro Ser Phe Thr Val Pro His Tyr Glu Val Ile Leu Leu Pro Ile Phe
145 150 155 160
Ala Gln Val Ala Asn Leu His Leu Leu Leu Leu Arg Asp Ile Glu Ile
165 170 175
Phe Gly Leu Glu Trp Gly Met Ser Lys Ala Glu Ile Asp Asp Tyr Tyr
180 185 190
Phe Ala Asn Ser Gly Leu Thr Gly Leu Thr Gln Lys Tyr Thr Asn His
195 200 205
Ile Val Lys Trp Tyr Ser Glu Gly Leu Cys Ile Ala Thr Asn Ile Asp
210 215 220
Leu Gly Gln Cys Pro Glu Phe Tyr Gln Leu Asp Lys Trp Asn Ala Met
225 230 235 240
Asn Asp Phe Arg Arg Glu Met Thr Phe Met Val Leu Asp Ile Ile Ala
245 250 255
Leu Trp Pro Thr Tyr Asp Pro Ile Arg Tyr Pro Leu Gly Ile Lys Thr
260 265 270
Glu Leu Thr Arg Glu Val Phe Thr Pro Leu Leu Gly Ile Asn Pro Asn
275 280 285
Ser Ser Ser Leu Ile His Thr Met Glu Glu Ile Glu Asp Lys Leu Thr
290 295 300
Phe Leu Ser Pro Phe Leu Ser Trp Ile Ser Phe Glu Gln Leu Val Lys
305 310 315 320
Gln Gly Asp Gly Ile Ser Thr Phe Thr Asp Trp Gly Asn Phe Thr Leu
325 330 335
Ser Asn Thr Met Leu Pro Ile Ser Tyr Ile Leu Gly Gly Ala Gly Ser
340 345 350
Gly Thr Gly Asp Ser Ile Asn Ile Pro Ile Lys Ser Glu Asn Tyr Asp
355 360 365
Val Tyr Lys Val Arg Val Gly Thr Asp Tyr Ser His Pro Ser Asn Val
370 375 380
Pro Ile Arg Lys Leu Glu Tyr Tyr Cys Thr Asn Gly Thr Met Glu Asn
385 390 395 400
Val Ile Thr Val Gly Thr Gly Thr Thr Asn Ala Leu Phe Glu Leu Pro
405 410 415
Asn Asn Gly Tyr Ile Asp Tyr Ser His Arg Val Ser Arg Leu Ser Cys
420 425 430
Ser Asn Val Glu Val Tyr Glu Trp Glu Gly Gly Pro Lys Tyr Ala Leu
435 440 445
Lys Asn Ile Ala Tyr Gly Trp Thr His Ile Ser Val Asp Ser Lys Asn
450 455 460
Thr Leu Ser Ala Asn Ala Ile Thr Gln Ile Pro Ala Arg Lys Gly Tyr
465 470 475 480
Ser Ser Ser Glu Ser Asn Leu Ser Ile Ala Gly Pro Tyr Phe Thr Gly
485 490 495
Gly Asp Leu Ile Ser Leu Pro Pro Asn Gly Ala Gln Leu Gln Met Arg
500 505 510
Val Thr Pro Pro Val Ser Ser Ser Thr Thr Asn Tyr Cys Val Arg Leu
515 520 525
Arg Tyr Ala Ser Ser Gly Asn Thr Asn Ile Tyr Val Glu Arg Val Leu
530 535 540
Ser Ser Gly Asp Thr Tyr Gly Glu Thr His Asn Val Pro Ala Thr Tyr
545 550 555 560
Ser Gly Gly Ser Leu Ser Tyr Ser Ser Phe Ala Tyr Val Val Asn Leu
565 570 575
Thr Ala Ile Phe Glu Gly Phe Asn Val Glu Ile Lys Ile Lys Asn Ile
580 585 590
Gly Ser Ser Gln Ile Ile Leu Asp Lys Ile Glu Phe Leu Pro Ile Lys
595 600 605
Glu Ser Leu Glu Gly Asn Lys Lys Leu Thr
610 615
<210> 61
<211> 616
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 61
Met Gly Lys Thr Ser Gly Glu Val Lys Met Leu Arg Thr Arg Gln Tyr
1 5 10 15
Arg Asn Ala Ser Glu Met Val Tyr Asp Gln Pro Leu Pro Phe Gly Gly
20 25 30
Gly Val Lys Ile Ala Gly Lys Ile Gly Leu Gly Val Val Lys Thr Val
35 40 45
Ile Thr Ser Ile Ile Arg Arg Gly Arg Asp Asn Glu Ile Ala Gly Arg
50 55 60
Ile Leu Ser Asp Val Tyr Asn Ile Leu Trp Ser Asn His Lys Gly Tyr
65 70 75 80
Trp Asn Glu Met Ile Glu Ala Val Glu Thr Leu Ile Gln His Asp Ile
85 90 95
Ser Glu Asn Ile Arg Asn Asn Ala Leu Ala Val Leu Thr Asp Val Arg
100 105 110
Asn Pro Leu Leu Gln Tyr Gln Gln Ala Val Asp Asp Trp Gln Arg Asn
115 120 125
Arg Thr Asp Ser Lys Leu Gln Glu Gln Val Arg Asn Gln Leu Ile Ala
130 135 140
Thr Asn Thr Phe Ile Glu Phe Ala Met Pro Ser Phe Thr Val Pro His
145 150 155 160
Tyr Glu Val Ile Leu Leu Pro Ile Phe Ala Gln Val Ala Asn Leu His
165 170 175
Leu Leu Leu Leu Arg Asp Ile Glu Ile Phe Gly Leu Glu Trp Gly Met
180 185 190
Ser Lys Ala Glu Ile Asp Asp Tyr Tyr Phe Ala Asn Ser Gly Leu Thr
195 200 205
Gly Leu Thr Gln Lys Tyr Thr Asn His Ile Val Lys Trp Tyr Ser Glu
210 215 220
Gly Leu Cys Ile Ala Thr Asn Ile Asp Leu Gly Gln Cys Pro Glu Phe
225 230 235 240
Tyr Gln Leu Asp Lys Trp Asn Ala Met Asn Asp Phe Arg Arg Glu Met
245 250 255
Thr Phe Met Val Leu Asp Ile Ile Ala Leu Trp Pro Thr Tyr Asp Pro
260 265 270
Ile Arg Tyr Pro Leu Gly Ile Lys Thr Glu Leu Thr Arg Glu Val Phe
275 280 285
Thr Pro Leu Leu Gly Ile Asn Pro Asn Ser Ser Ser Leu Ile His Thr
290 295 300
Met Glu Glu Ile Glu Asp Lys Leu Thr Phe Leu Ser Pro Phe Leu Ser
305 310 315 320
Trp Ile Ser Phe Glu Gln Leu Val Lys Gln Gly Asp Gly Ile Ser Thr
325 330 335
Phe Thr Asp Trp Gly Asn Phe Thr Leu Ser Asn Thr Met Leu Pro Ile
340 345 350
Ser Tyr Ile Leu Gly Gly Ala Gly Ser Gly Thr Gly Asp Ser Ile Asn
355 360 365
Ile Pro Ile Lys Ser Glu Asn Tyr Asp Val Tyr Lys Val Arg Val Gly
370 375 380
Thr Asp Tyr Ser His Pro Ser Asn Val Pro Ile Arg Lys Leu Glu Tyr
385 390 395 400
Tyr Cys Thr Asn Gly Thr Met Glu Asn Val Ile Thr Val Gly Thr Gly
405 410 415
Thr Thr Asn Ala Leu Phe Glu Leu Pro Asn Asn Gly Tyr Ile Asp Tyr
420 425 430
Ser His Arg Val Ser Arg Leu Ser Cys Ser Asn Val Glu Val Tyr Glu
435 440 445
Trp Glu Gly Gly Pro Lys Tyr Ala Leu Lys Asn Ile Ala Tyr Gly Trp
450 455 460
Thr His Ile Ser Val Asp Ser Lys Asn Thr Leu Ser Ala Asn Ala Ile
465 470 475 480
Thr Gln Ile Pro Ala Arg Lys Gly Tyr Ser Ser Ser Glu Ser Asn Leu
485 490 495
Ser Ile Ala Gly Pro Tyr Phe Thr Gly Gly Asp Leu Ile Ser Leu Pro
500 505 510
Pro Asn Gly Ala Gln Leu Gln Met Arg Val Thr Pro Pro Val Ser Ser
515 520 525
Ser Thr Thr Asn Tyr Cys Val Arg Leu Arg Tyr Ala Ser Ser Gly Asn
530 535 540
Thr Asn Ile Tyr Val Glu Arg Val Leu Ser Ser Gly Asp Thr Tyr Gly
545 550 555 560
Glu Thr His Asn Val Pro Ala Thr Tyr Ser Gly Gly Ser Leu Ser Tyr
565 570 575
Ser Ser Phe Ala Tyr Val Val Asn Leu Thr Ala Ile Phe Glu Gly Phe
580 585 590
Asn Val Glu Ile Lys Ile Lys Asn Ile Gly Ser Ser Gln Ile Ile Leu
595 600 605
Asp Lys Ile Glu Phe Leu Pro Ile
610 615
<210> 62
<211> 1183
<212> БЕЛОК
<213> Bacillus sp.
<400> 62
Met Asn Asn Glu Asn Thr Asn Asn Val Asn Ser Phe Cys Thr Pro Asp
1 5 10 15
Pro Ser Asp Val Ser Leu Ser Ser Gln Arg Tyr Pro Tyr Ala Asn Asp
20 25 30
Pro Thr Ala Pro Leu Leu Ser Ala Asp Asp Cys Leu Leu Ser Tyr Gly
35 40 45
Glu Phe Gly Ile Asp Ser Ser Thr Ser Leu Glu Pro Phe Ile Asp Ala
50 55 60
Ser Gly Ala Ile Asn Ala Ala Ile Gly Val Thr Gly Thr Ile Leu Gly
65 70 75 80
Phe Leu Gly Val Pro Phe Ala Gly Ala Leu Thr Thr Phe Tyr Gln Lys
85 90 95
Leu Phe Gly Phe Leu Phe Pro Ser Lys Asp Thr Glu Gln Trp Glu Gln
100 105 110
Phe Met Lys Gln Val Glu Val Leu Ile Asp Lys Lys Ile Ala Asp Ala
115 120 125
Val Arg Asn Lys Ala Ile Ala Glu Leu Gln Gly Leu Ile Asn Asn Leu
130 135 140
Thr Leu Tyr Thr Glu Ala Leu Glu Asp Trp Leu Glu Asp Lys Glu Asn
145 150 155 160
Gly Ala Ala Arg Asp Arg Val Leu Gln Arg Trp Arg Asn Leu Asp Ser
165 170 175
Leu Phe Glu Gln Gln Ile Pro Ser Phe Ala Ile Lys Asp Phe Glu Val
180 185 190
Leu Leu Leu Val Val Tyr Ala Gln Ala Thr Asn Leu Gln Leu Leu Ser
195 200 205
Leu Lys Asp Ala Tyr Ile Tyr Gly Ala Asp Trp Gly Leu Thr Pro Thr
210 215 220
Asn Val Asp Gln Ile His Thr Arg Leu Leu Arg Asn Ser Ala Lys Tyr
225 230 235 240
Ala Asp His Cys Val Lys Trp Tyr Asn Thr Gly Leu Glu Asn Leu Lys
245 250 255
Gly Ala Asn Ala Ala Ser Trp Val Lys Tyr Asn Arg Phe Arg Arg Glu
260 265 270
Met Thr Leu Ser Val Leu Asp Val Ile Ala Leu Phe Pro Met Tyr Asp
275 280 285
Met Lys Val Tyr Pro Ile Ala Thr Thr Pro Glu Leu Thr Arg Glu Ile
290 295 300
Tyr Met Asp Val Leu Ala Asp Asp Ala Met Ser Leu Gly Thr Arg Tyr
305 310 315 320
Phe Trp Tyr Ser Glu Asn Ala Pro Ser Phe Ser Ser Leu Glu Asn Thr
325 330 335
Leu Ile Arg Ala Pro His Ser Val Asp Phe Ile Thr Glu Leu Glu Ile
340 345 350
Phe Tyr Ala Glu Arg Ser Tyr Gly Leu Phe Tyr Glu Tyr Ala Gly Arg
355 360 365
Trp Val Gly His Asn Ile Lys Thr Lys Gly Ile Asn His Asn Asn Gly
370 375 380
Ser Ile Arg Leu Gln Tyr Gly Ala Thr Thr Asp Asn Ile Tyr Lys Ile
385 390 395 400
Ser Leu Asn Gly Arg Glu Ile Tyr Lys Thr Gly Ser Gln Ser Leu Ala
405 410 415
Met Pro Thr Thr Asp Ile Gly Asn Thr His Pro Ile Tyr Phe Ile Thr
420 425 430
Arg Ala Val Asn Phe Phe Ala Val Ser Gly Ser His Ile Ser Val Asn
435 440 445
Lys Phe Tyr Ser Lys Lys Asp Ser Trp Tyr Gln Glu Val Gly Gly Leu
450 455 460
Pro Glu Asn Val Gly Tyr Phe Ser Thr Ala Glu Leu Pro Pro Asp Arg
465 470 475 480
Ala Asp Glu Pro Glu His Leu Ala Tyr Ser His Arg Leu Cys His Val
485 490 495
Thr Phe Ile Gly Val Ser Asn Gly Asn Lys Tyr Ser Lys Asp Leu Pro
500 505 510
Leu Phe Ser Trp Thr His Arg Ser Leu Ser Pro Ser Ile Arg Val His
515 520 525
Lys Asp Arg Ile Thr Gln Ile Pro Val Val Lys Gln Lys Pro Thr Pro
530 535 540
Val Arg Phe Val Lys Gly Ser Gly Tyr Thr Gly Gly Asp His Ile Leu
545 550 555 560
Val Asn Ala Asp Asn Ser Thr Gly Ser Asp Leu Phe Asn Phe Tyr Phe
565 570 575
Asp Val Ala Gly Leu Asp Phe Thr Lys Lys Tyr Arg Ile Arg Leu Arg
580 585 590
Tyr Ala Ala Asp Gly Asp Asn Lys Val Asn Leu Tyr Lys Thr Ala Ile
595 600 605
Gln Gly Gly Glu Gly Ser Ser Phe Ser Leu Pro Ala Thr Met Asn Glu
610 615 620
Gly Ala Asp Leu Thr Tyr Asn Ser Phe Gln Phe Ile Glu Phe Pro Gly
625 630 635 640
Val Tyr Asn Phe Pro Gly Pro Tyr Phe Thr Ser Leu Arg Ala Ser Ile
645 650 655
Pro Phe Gly Ser Val Lys Gly Asn Val Tyr Ser Asp Arg Leu Glu Phe
660 665 670
Ile Pro Val Asp Asp Asn Tyr Glu Asp Glu Leu Thr Leu Glu Glu Ala
675 680 685
Lys Lys Ala Val Asn Asp Leu Phe Thr Asp Ala Arg Asn Thr Leu Lys
690 695 700
Ile Asp Val Thr Asp Tyr Gln Ile Asp Gln Ala Ala Asn Leu Val Glu
705 710 715 720
Cys Ile Ser Asp Asp Leu Tyr Gly Lys Glu Lys Ile Met Leu Leu Arg
725 730 735
Ala Val Lys Phe Val Lys Gln Leu Ser Gln Ser Arg Asn Leu Leu Ser
740 745 750
Asp Pro Glu Phe Asn Asn Val Asn Arg Glu Asp Ser Trp Thr Ala Asn
755 760 765
Thr Gly Val Ser Ile Leu Glu Gly Asp Pro Leu Tyr Lys Glu Arg Ala
770 775 780
Ala Gln Leu Ser Ser Ala Arg Asp Glu Arg Phe Pro Thr Tyr Leu Tyr
785 790 795 800
Gln Lys Ile Asp Glu Ser Lys Leu Lys Pro Tyr Thr Arg Tyr Gln Leu
805 810 815
Arg Gly Phe Val Glu Gly Ser Glu Asn Leu Asp Val Tyr Leu Ile Arg
820 825 830
Tyr Asp Ala Lys His Val Arg Met Asn Val Pro Tyr Asn Leu Glu Ile
835 840 845
Leu Asp Asn Ser Ser Pro Leu Ser Pro Cys Glu Glu Ile Asp Asp Arg
850 855 860
Ser Asn Ser Ser Cys Asn Glu Phe Asp Arg Cys Lys Gln Ser Ile Val
865 870 875 880
Val Asp Pro Asp Thr Asn Thr Asp Thr Asp Gln Leu Glu Arg Asp Pro
885 890 895
His Ala Phe Ser Phe His Ile Asp Thr Gly Thr Val Asp Ser Thr Glu
900 905 910
Asp Ile Gly Ile Trp Val Ala Phe Lys Ile Ser Glu Leu Asp Gly Ser
915 920 925
Ala Ile Leu Gly Asn Pro Glu Leu Ile Glu Val Gly Pro Leu Ser Ser
930 935 940
Glu Ala Val Ala Gln Val Gln Arg Glu Glu Glu Gln Trp Lys Gln Val
945 950 955 960
Leu Ala Lys Lys Arg Glu Lys Thr Ala Lys Leu Tyr Ala Ala Ala Lys
965 970 975
Gln Ala Ile Asp Arg Leu Phe Ala Asp Pro Gln Asp Thr Thr Leu Arg
980 985 990
Phe Asp Thr Glu Phe Ser Asn Ile Leu Ser Ala Asp His Leu Val Tyr
995 1000 1005
Lys Ile Gln Asp Ile Tyr Asn Ser Glu Leu Thr Ala Ile Pro Gly
1010 1015 1020
Leu Asn Tyr Asp Leu Phe Met Glu Leu Glu Asn Arg Ile Gln Asn
1025 1030 1035
Ala Ile Asp Leu Tyr Asp Ala Arg Asn Ile Leu Gln Asn Gly Glu
1040 1045 1050
Phe Arg Asn Gly Val Thr Ser Trp Glu Thr Thr Ala Asn Val Glu
1055 1060 1065
Val Gln Gln Val Glu Asp Ala Ser Val Leu Val Val Ser Asn Trp
1070 1075 1080
Asn Ala Ser Val Ala Gln Ser Val Ser Val Gln Lys Asp His Gly
1085 1090 1095
Tyr Ile Leu Arg Val Thr Ala Lys Lys Glu Gly Ser Gly Asn Gly
1100 1105 1110
Tyr Val Thr Ile Leu Asp Cys Ala Asn His Ile Asp Thr Leu Thr
1115 1120 1125
Phe Ser Ala Cys Asp Ser Gly Ser Asp Leu Ser Ser Asn Glu Leu
1130 1135 1140
Ala Gly Tyr Val Thr Lys Thr Leu Glu Ile Tyr Pro Asp Thr Asp
1145 1150 1155
Gln Ile Arg Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Tyr Val
1160 1165 1170
Glu Ser Val Asp Leu Ile Arg Met Glu Gly
1175 1180
<210> 63
<211> 675
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 63
Met Asn Asn Glu Asn Thr Asn Asn Val Asn Ser Phe Cys Thr Pro Asp
1 5 10 15
Pro Ser Asp Val Ser Leu Ser Ser Gln Arg Tyr Pro Tyr Ala Asn Asp
20 25 30
Pro Thr Ala Pro Leu Leu Ser Ala Asp Asp Cys Leu Leu Ser Tyr Gly
35 40 45
Glu Phe Gly Ile Asp Ser Ser Thr Ser Leu Glu Pro Phe Ile Asp Ala
50 55 60
Ser Gly Ala Ile Asn Ala Ala Ile Gly Val Thr Gly Thr Ile Leu Gly
65 70 75 80
Phe Leu Gly Val Pro Phe Ala Gly Ala Leu Thr Thr Phe Tyr Gln Lys
85 90 95
Leu Phe Gly Phe Leu Phe Pro Ser Lys Asp Thr Glu Gln Trp Glu Gln
100 105 110
Phe Met Lys Gln Val Glu Val Leu Ile Asp Lys Lys Ile Ala Asp Ala
115 120 125
Val Arg Asn Lys Ala Ile Ala Glu Leu Gln Gly Leu Ile Asn Asn Leu
130 135 140
Thr Leu Tyr Thr Glu Ala Leu Glu Asp Trp Leu Glu Asp Lys Glu Asn
145 150 155 160
Gly Ala Ala Arg Asp Arg Val Leu Gln Arg Trp Arg Asn Leu Asp Ser
165 170 175
Leu Phe Glu Gln Gln Ile Pro Ser Phe Ala Ile Lys Asp Phe Glu Val
180 185 190
Leu Leu Leu Val Val Tyr Ala Gln Ala Thr Asn Leu Gln Leu Leu Ser
195 200 205
Leu Lys Asp Ala Tyr Ile Tyr Gly Ala Asp Trp Gly Leu Thr Pro Thr
210 215 220
Asn Val Asp Gln Ile His Thr Arg Leu Leu Arg Asn Ser Ala Lys Tyr
225 230 235 240
Ala Asp His Cys Val Lys Trp Tyr Asn Thr Gly Leu Glu Asn Leu Lys
245 250 255
Gly Ala Asn Ala Ala Ser Trp Val Lys Tyr Asn Arg Phe Arg Arg Glu
260 265 270
Met Thr Leu Ser Val Leu Asp Val Ile Ala Leu Phe Pro Met Tyr Asp
275 280 285
Met Lys Val Tyr Pro Ile Ala Thr Thr Pro Glu Leu Thr Arg Glu Ile
290 295 300
Tyr Met Asp Val Leu Ala Asp Asp Ala Met Ser Leu Gly Thr Arg Tyr
305 310 315 320
Phe Trp Tyr Ser Glu Asn Ala Pro Ser Phe Ser Ser Leu Glu Asn Thr
325 330 335
Leu Ile Arg Ala Pro His Ser Val Asp Phe Ile Thr Glu Leu Glu Ile
340 345 350
Phe Tyr Ala Glu Arg Ser Tyr Gly Leu Phe Tyr Glu Tyr Ala Gly Arg
355 360 365
Trp Val Gly His Asn Ile Lys Thr Lys Gly Ile Asn His Asn Asn Gly
370 375 380
Ser Ile Arg Leu Gln Tyr Gly Ala Thr Thr Asp Asn Ile Tyr Lys Ile
385 390 395 400
Ser Leu Asn Gly Arg Glu Ile Tyr Lys Thr Gly Ser Gln Ser Leu Ala
405 410 415
Met Pro Thr Thr Asp Ile Gly Asn Thr His Pro Ile Tyr Phe Ile Thr
420 425 430
Arg Ala Val Asn Phe Phe Ala Val Ser Gly Ser His Ile Ser Val Asn
435 440 445
Lys Phe Tyr Ser Lys Lys Asp Ser Trp Tyr Gln Glu Val Gly Gly Leu
450 455 460
Pro Glu Asn Val Gly Tyr Phe Ser Thr Ala Glu Leu Pro Pro Asp Arg
465 470 475 480
Ala Asp Glu Pro Glu His Leu Ala Tyr Ser His Arg Leu Cys His Val
485 490 495
Thr Phe Ile Gly Val Ser Asn Gly Asn Lys Tyr Ser Lys Asp Leu Pro
500 505 510
Leu Phe Ser Trp Thr His Arg Ser Leu Ser Pro Ser Ile Arg Val His
515 520 525
Lys Asp Arg Ile Thr Gln Ile Pro Val Val Lys Gln Lys Pro Thr Pro
530 535 540
Val Arg Phe Val Lys Gly Ser Gly Tyr Thr Gly Gly Asp His Ile Leu
545 550 555 560
Val Asn Ala Asp Asn Ser Thr Gly Ser Asp Leu Phe Asn Phe Tyr Phe
565 570 575
Asp Val Ala Gly Leu Asp Phe Thr Lys Lys Tyr Arg Ile Arg Leu Arg
580 585 590
Tyr Ala Ala Asp Gly Asp Asn Lys Val Asn Leu Tyr Lys Thr Ala Ile
595 600 605
Gln Gly Gly Glu Gly Ser Ser Phe Ser Leu Pro Ala Thr Met Asn Glu
610 615 620
Gly Ala Asp Leu Thr Tyr Asn Ser Phe Gln Phe Ile Glu Phe Pro Gly
625 630 635 640
Val Tyr Asn Phe Pro Gly Pro Tyr Phe Thr Ser Leu Arg Ala Ser Ile
645 650 655
Pro Phe Gly Ser Val Lys Gly Asn Val Tyr Ser Asp Arg Leu Glu Phe
660 665 670
Ile Pro Val
675
<210> 64
<211> 385
<212> БЕЛОК
<213> Bacillus sp.
<400> 64
Met Ala Ile Glu Ile Lys Gln Asn Lys Phe Tyr Glu Ile Arg Pro Ala
1 5 10 15
Lys Asp Ile Ser Lys Ile Val Gln Val Gly Trp Gly Ala Asn Ser Met
20 25 30
Gly Asp Thr Phe Glu Val Tyr Pro Pro Gly Arg Tyr Pro Ala Asp Trp
35 40 45
His Lys Phe Leu Ile Val Pro Leu Asp Asp Asp Phe Tyr Ala Ile Val
50 55 60
Cys Lys Asn Ser Gly Leu Val Met Lys Val Ser Arg Asp Asp Asn Asn
65 70 75 80
Asp Gly Arg Trp Ile Glu Gln Trp Glu Trp Leu Asp Glu Glu Trp Glu
85 90 95
Gln Ala Arg Glu Ser Val Phe Phe Gln Glu Val Gly Ser Asn Thr Tyr
100 105 110
Ala Leu Leu Tyr Glu Gly Thr Gly Lys Tyr Met Thr Leu Ser Gly Arg
115 120 125
Ser Ser His Asp Gly Asn Val Cys Glu Gln Trp Glu Trp Glu Asp Gly
130 135 140
Asn Trp Gln His Trp Lys Ile Asn Glu Leu Ser Asp Ser Ile Val Leu
145 150 155 160
Pro Glu Ile Glu Thr Ala Leu Pro Pro Val Ala Pro Asp Tyr Asp Pro
165 170 175
Ala Gly Gly Ile Asn Gln Val Leu Pro Glu Lys Ser Asp Thr Val Ile
180 185 190
Thr Asp Thr Ala Leu Leu Pro Phe Phe Met Val Asn Asp Pro His Tyr
195 200 205
Thr Asp Val Leu Lys Lys Phe Gln Asn Asn Pro Tyr Tyr Leu Leu Val
210 215 220
Lys Lys Gln Tyr Tyr Glu Lys Thr Ala Ser Gln Ile Leu Ala Pro Ser
225 230 235 240
Glu Val Tyr Ala Tyr Thr Glu Thr Ser Gly Ile Thr Glu Ile Glu Gln
245 250 255
Asn Glu Phe Glu Gln Ile Val Asn Met Ser Ile Gly Met Asp Tyr Gly
260 265 270
Leu Gln Phe Lys Lys Phe Ser Ala Ser Leu Lys Tyr Glu Ile Ser Asn
275 280 285
Ser Ile Arg Thr Leu Ile Ser Lys Ser Ile Glu Arg Arg Thr Glu Thr
290 295 300
Ala Thr Thr Arg Thr Ile Thr Asn Pro Leu Ala Gln Pro Ala Ser Trp
305 310 315 320
Thr Gln Tyr Gln Leu Val Thr Glu Tyr Gln Val Leu Arg Ala Asn Gly
325 330 335
Thr Pro Leu Asp Ser Pro Trp Ser Val Lys Leu Asp Asn His Ser Val
340 345 350
Ser Arg Phe Tyr Pro Asn Thr Thr Ser Leu Leu Glu Ser Thr Thr Gln
355 360 365
Arg Glu Leu Leu Glu Ala Leu Lys Asn Lys Leu Thr Lys Cys Cys Leu
370 375 380
Gln
385
<210> 65
<211> 223
<212> БЕЛОК
<213> Bacillus sp.
<400> 65
Val Glu Glu Pro Gln Tyr Ile Lys Tyr Lys Lys Glu Arg Arg Asp Ile
1 5 10 15
Gly Met Asp Ser Gly Ile Ile Ser Asp Glu Arg Lys Asn Asp Tyr Ser
20 25 30
Gly Asp Ala Ser Phe Leu Lys Leu Ile Ser Asp Val Asn Pro Glu Tyr
35 40 45
Asn Leu Gln Ser Leu Lys Leu Ile Asp Lys Phe Asn Ser Ile Val Asp
50 55 60
Pro Ile Thr Arg Glu Leu Asp Phe Gly Lys Ile Leu Gln Ile Ala Asn
65 70 75 80
Glu Asn Pro Asp Leu Val Ile Val Ser Thr Leu Asn His Gln Leu Ala
85 90 95
Ser Asn Gln Lys Met Gln Ile Asp Asn Leu Ile Asp Leu Ser Lys Asn
100 105 110
Phe Ile Leu Ser Ala Leu Gly Gly Thr Tyr Leu Asn Glu Gly Ile Gln
115 120 125
Lys Thr Cys Thr Asp Trp Ile Gly Asp Ile Phe Thr Asn Leu Asn Ser
130 135 140
Gln Ile Gln His Ser Trp Ile Ser Phe Tyr Glu Glu Thr Arg Asn Lys
145 150 155 160
Thr Ser Tyr Arg Tyr Asn Ile Phe Phe Thr Phe Gln Arg Asp Ser Phe
165 170 175
Leu Val Val Leu Pro Ile Gly Phe Asp Ile Thr Val His Ala Asn Lys
180 185 190
Glu Thr Val Leu His Leu Lys Pro Thr Asp Ser Ala Ala Tyr Asp Val
195 200 205
Ser Ile Lys Gln Ile Gln Ile Ile Gln Pro Leu Asn Pro Gln Leu
210 215 220
<210> 66
<211> 966
<212> БЕЛОК
<213> Bacillus sp.
<400> 66
Val Met Lys Val Val Ser Asn Ser Met Val Pro Tyr Asn Val Leu Arg
1 5 10 15
Asn Met Asn Met Pro Thr Ile Ser Gly Thr Arg Trp Asn Lys Glu Met
20 25 30
Phe Ser Ser Ala Ile Asp Asn Thr Ser Val Leu Leu Asp Leu Ile Glu
35 40 45
Lys Gly Ile Asn Asp Gly Asp Asp Leu Leu Gly Leu Phe Ser Phe Ile
50 55 60
Gly Leu Thr Ala Leu Glu Ala Ile Pro Ile Val Gly Gly Thr Leu Ser
65 70 75 80
Lys Leu Ile Ser Ile Ile Phe Phe Ser Ser Lys Ser Ser Ile Asn Pro
85 90 95
Gln Lys Ile Trp Glu Glu Leu Gly Lys Ala Ile Asn Gln Ile Ile Asp
100 105 110
Lys Lys Ile Glu Glu Thr Leu Ile Ser Gly Leu Ile Gln Glu Ile Ser
115 120 125
Gly Phe Ala Asn Val Leu Glu Glu Tyr Arg Ala Ala Tyr Asp Leu Tyr
130 135 140
Asn Gly Lys Ser Val Phe Glu Thr Pro Asp Asn Leu Thr Pro Gly Glu
145 150 155 160
Tyr Leu Val Asn Val Phe Thr Thr Ala Asn Leu Leu Phe Ile Gln Arg
165 170 175
Ile Pro Lys Phe Gln Asp Pro Asp His Asp Val Leu Phe Leu Pro Phe
180 185 190
Phe Val His Ile Ala Glu Met His Ile Leu Leu Ile Arg Asp Ala Ala
195 200 205
Met His Gly Leu Glu Trp Gly Met Asp Glu Lys Ile His Gln Lys Phe
210 215 220
Lys Lys Asp Phe Lys Ser Tyr Ile Gly Glu Tyr Ser Ala Tyr Leu Leu
225 230 235 240
Lys Ile Tyr Lys Lys Gly Leu Lys Glu Ala Ser Glu Arg Glu Leu Asn
245 250 255
Asp Lys Asp Phe Pro Thr Ile Lys Asp Lys Asn His Tyr Ile Asn Thr
260 265 270
Val Arg Trp Asn Val Ile Asn Gln Tyr Lys Arg Gly Met Thr Leu Thr
275 280 285
Ala Phe Asp Phe Ala Tyr Lys Trp Lys Tyr Tyr His Glu Val Tyr Gln
290 295 300
Asn Asn Ile Thr Leu Asn Pro Phe Arg Thr Ile Tyr Ser Asp Ile Ala
305 310 315 320
Gly Ser Val Tyr Pro Tyr Ile Lys Thr Thr Asn Glu Ile Asn Asn Thr
325 330 335
Ile Lys Asn Gln Asn Leu Lys Tyr Arg Gly Ile Phe Lys Lys Leu Gln
340 345 350
Val Tyr His Ala Glu Arg Ile Asp Ser Ile Gln Ser Thr Tyr Ile Arg
355 360 365
Asn Asn Glu Ile Ile Asn Ser Thr Lys Ile Gly Gly Val Gly Gly Arg
370 375 380
Ile Ser Ser Leu Val Leu Glu Glu Pro Ile Lys Asn Pro Leu Ile Gln
385 390 395 400
Val Asn Met Trp Ser Glu Leu Val Pro Ile Ser Leu Gly Phe Lys Phe
405 410 415
Tyr Asp Glu Lys Glu Gln Asn Ile Gly Gly Gly Ser Trp Trp Pro Lys
420 425 430
Pro His Lys Phe Gly Ala Tyr His Phe Val Gly Asn Lys Val Ser Ser
435 440 445
Ile Ile Gly Phe Gly Lys Asn Glu Thr Gly Gly Phe Asn Ser Leu Asp
450 455 460
Ala Met Ile Val Gly Phe Lys Arg Asp Asp Tyr Thr Pro Glu Asn Arg
465 470 475 480
Leu Leu Gly Ile Asn Glu Thr Gly Glu Pro Val Thr Lys Val Ile Asp
485 490 495
Ala Gly Asn Phe Gln Gln Glu Lys Phe Gly Ser Asn Ile Gln Ile Thr
500 505 510
Asp Glu Pro Met Phe Gly Asp Gly Val Leu Gln Phe Thr Asn Tyr Ala
515 520 525
Asn Thr Met Asn Gln Asp Ser Ser Val Thr Tyr Gln Ile Asn Ile Glu
530 535 540
Ile Glu Gly Ile Tyr Gln Leu His Ala Ile Ile Gly Ala Lys Lys Gln
545 550 555 560
Lys Asp Lys Leu Ser Phe Lys Val Ile Leu Asn Thr Lys Gln Gln Lys
565 570 575
Lys Ile Ile Thr Asp Ser Phe Asp Ser His Asp Ile Trp Glu Gly Met
580 585 590
Ser Leu Asn Glu Ser Ile Val Tyr Lys Arg Ile Leu Ile Gly Thr Phe
595 600 605
Pro Leu Lys Arg Gly Met Asn Ser Ile Thr Ile His Asn Asp Val Leu
610 615 620
Gln Ser Ser Ala Asn Ile Lys Thr Trp Asn Leu Gly Ser Leu Glu Leu
625 630 635 640
Thr Met Asp Ser Asp Ser Ile Lys Asp Pro Asp Ile Thr Thr Leu Tyr
645 650 655
Asp Lys Asp Asn Tyr Ala Gly Thr Lys Lys Tyr Ile Phe Glu Asp Thr
660 665 670
Ala Arg Leu Ser Asp Phe Asn Asp Arg Thr Ser Ser Ile Lys Val Gly
675 680 685
Ser His Ile Ala Gly Val Arg Phe Tyr Glu His Tyr His Tyr Ser Gly
690 695 700
Asp Phe Ile Asp Leu Gly Asn Gly Glu Lys Leu Ser Leu Lys Asn His
705 710 715 720
Arg Leu Asn Lys Arg Leu Ser Ser Val Lys Phe Ala Asn Leu Gly Leu
725 730 735
Tyr Lys Glu Asp Asn Tyr Lys Gly Glu Lys Lys Leu Ile Phe Glu Asp
740 745 750
Ile Pro Tyr Leu Thr Asp Phe Asn Asp Lys Thr Ser Ser Ile Val Val
755 760 765
Ser Pro Asn Val Pro Gly Thr Val Arg Leu Phe Glu His Ser Tyr Tyr
770 775 780
Asn Gly Asp Tyr Ile Glu Val Ser Gly Gly Gln Lys Leu Ser Leu Lys
785 790 795 800
Gly His Asn Leu Asn Arg Lys Ile Ser Ser Ile Lys Phe Leu Lys Glu
805 810 815
His Gln Val Phe Asn Gly Leu Tyr Glu Ile Val Thr Ala Ile Asn Lys
820 825 830
Lys Ser Val Val Asp Trp Ser Gln Asp Lys Asn Ser Asn Val His Leu
835 840 845
Trp Glu Gln Arg Asp Arg Asp Asn Gln Lys Trp Tyr Phe Asp Tyr Asp
850 855 860
Ser Ser Lys Gln Ala Phe Gln Ile Lys Ser Lys Ala Asp Lys Asn Lys
865 870 875 880
Val Leu Ala Trp Asn Asp Phe Gly Gly Ser Lys Asn Val Phe Ala Thr
885 890 895
Pro Asn Leu Lys Lys Asp Glu His Phe Trp Thr Phe Asp Tyr Leu Gly
900 905 910
Glu Gly Asn Tyr Leu Ile Arg Asn Lys Lys Asp Gln Asn Leu Val Leu
915 920 925
Asp Val Asp Gly His Gln Thr Ala Asn Gly Thr Asn Ile Lys Val Asn
930 935 940
Ala Gln His Ala Leu Asp Asn Pro Ser Ile Glu Ala Gln Lys Phe Lys
945 950 955 960
Leu Asn His Val Ile Asn
965
<210> 67
<211> 192
<212> БЕЛОК
<213> Streptomyces sp.
<400> 67
Leu Ala Ala Ser Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu
1 5 10 15
Glu Arg Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Phe Asp Phe Glu Ala Ile Arg Ala Ala Ala Ala Ala
35 40 45
Val Pro Asp Thr Thr Val Val Arg Gln Val Arg Gly Trp Gly Leu Gln
50 55 60
Glu His Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Pro Glu Tyr Ala Glu Ala Gly Phe Trp Gly Thr
85 90 95
Val Glu Arg Glu Leu Val Glu Ala Phe Thr Gly Leu Ala Gly Gln Glu
100 105 110
Gly Gln Pro Gly Leu Ser Phe Tyr Glu Glu Ser Asp Gln Arg Thr Arg
115 120 125
Phe Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp
130 135 140
Asp Ile Tyr Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro
145 150 155 160
Lys Ala Glu Ala Thr Thr Met Glu Leu Thr Asp Val Val Pro Phe Ala
165 170 175
Val Arg Leu Asn Ala Ile Leu Val Arg Gln Ala Pro Thr Val Ala Val
180 185 190
<210> 68
<211> 483
<212> БЕЛОК
<213> Streptomyces sp.
<400> 68
Leu Arg Gly Asp Arg Leu Pro Ala Arg Pro Arg Pro Arg Gln His Val
1 5 10 15
Leu Pro Ala Arg Leu Arg Ala Arg Gly Asp Ala Leu Glu Val Gly Ala
20 25 30
Asp Leu Gly Ala Ala Ala Ala Arg Arg Ala Ala Gln Ala Arg Ala Gly
35 40 45
His Ala Arg Arg Gly Leu Arg Ala Ala Glu Gly Arg Arg Arg Arg Arg
50 55 60
Arg Ala Pro Ala Ala Ala Gly Arg Arg Arg Gly Pro Glu Arg Asp Pro
65 70 75 80
Ala Val Arg Gly Asp Glu Arg Gly Asp Pro Ala Gln Gly Pro Pro Leu
85 90 95
Arg Arg Gly Arg Gly Ala Gly Pro Arg Gly Pro Gly Gly Gly Ala Gly
100 105 110
Ala Ala Pro Ala Arg Arg His Pro Arg Leu Gly Gly Ala Ala Arg Ala
115 120 125
Ala Arg Val Pro Ala Ala Arg Asp Asp Arg Arg Arg Gly Arg Ala Gly
130 135 140
Arg Arg Arg Gly Gly Thr Ala Arg Arg Pro Ala Val Ala Gly Ala Ala
145 150 155 160
Ala Pro Pro Gln Gly Pro His Val Pro His Asp Arg Arg Cys Ala Ala
165 170 175
Val Ala Gly Arg Ala Leu Leu Ala Glu Gly Arg Gly Gly Pro Arg Pro
180 185 190
Gly Gly Gly Val His Arg Asp Arg Ala Gly Pro Asp Gly Gln Gln His
195 200 205
Pro Gly Ala Ala Arg Gly Gly Pro Glu Gly Ala Glu Gly Arg Glu Gly
210 215 220
Gly Pro Glu Ala Arg Glu Glu Gly Gly Gly Gly Glu Ala Gly Pro Val
225 230 235 240
Pro Glu Ala Arg Glu Ala Arg Gln Gly Gln Gly Arg Arg Cys Arg Arg
245 250 255
Ala Arg Arg Glu Ala Pro Pro Arg Ala Leu Ser Gly Ala Leu Gly Ile
260 265 270
Leu Leu Leu Met Ile Asp Cys Pro Leu Arg Arg Ala Thr Arg Glu Gln
275 280 285
Glu Lys Pro Leu Ala Ala Ser Phe Arg Thr Val Val Glu Val Ala Pro
290 295 300
Asp Arg Leu Asp Glu Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala
305 310 315 320
Ile Ala Pro Ala Thr Val Asn Phe Asp Phe Glu Ala Ile His Arg Ala
325 330 335
Ala Ala Ala Val Pro Asp Ser Thr Ile Val Arg Met Val Arg Gly Trp
340 345 350
Gly Leu Lys Glu Tyr Ala Pro Leu Leu Val Met Val Leu Ser Leu Lys
355 360 365
Glu Ala Val Arg Gln Ala Leu Pro Ala Glu Cys Ala Glu Ala Gly Phe
370 375 380
Trp Glu Thr Val Glu Arg Glu Leu Val Ala Ala Phe Thr Gly Leu Gly
385 390 395 400
Glu Gln Glu Gly Gln Pro Gly Leu Ser Phe Tyr Glu Glu Ser Pro Glu
405 410 415
Arg Thr Arg Tyr Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Gly
420 425 430
Ala Gly Gly Ala Leu His Ala Leu Ala Phe Cys Val Asp Val Thr Val
435 440 445
Glu Leu Pro Lys Glu Arg Val Leu Gly Leu Gly Leu Thr Asp Thr Ala
450 455 460
Pro Phe Ala Val Gly Leu Asn Ala Ile Val Leu Arg Arg Pro Leu Ala
465 470 475 480
Val Ala Ala
<210> 69
<211> 383
<212> БЕЛОК
<213> Bacillus sp.
<400> 69
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala
20 25 30
Lys Asn Ala Tyr His Ser Ile His Leu Ala Ala Asn Ala Asn Leu Lys
35 40 45
Trp Asp Val Tyr Gly Asn Ser Pro Asn Asp Glu Ala Ala Ile Leu Leu
50 55 60
Tyr Asn Gly Ser Phe Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Ala Val
85 90 95
Thr Ile Gly Gly Gly Trp Ala Ala Pro Thr Asp Phe Thr Tyr Asn Arg
100 105 110
Glu Gly Leu Lys Gln Ser Lys Trp Thr Gly Ala Ala Asp Asn Lys Trp
115 120 125
Tyr Leu Arg Asp Gln Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly
130 135 140
Tyr Gly Lys Val Ala Ser Tyr Ala Arg Ile Gly Ile Thr Gly Pro Gln
145 150 155 160
Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn Asp Arg Leu Phe
165 170 175
His Ile Pro Ala Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu Pro
180 185 190
Thr Ile Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Ile Asp
195 200 205
Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu
210 215 220
Leu Pro Cys Ile Met Val Asn Asp Ser Gln Val Asp Asp Tyr Thr Lys
225 230 235 240
Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp
245 250 255
Lys Thr Phe Ser Lys Val Val Gln Pro Gly Leu Ser Glu Ser Tyr Ser
260 265 270
Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr
275 280 285
Leu Ser Met Lys Phe Gly Ala Asp Leu Gly Leu Lys Phe Gly Asp Ala
290 295 300
Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile
305 310 315 320
Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr Thr Ala
325 330 335
Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala
340 345 350
Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Thr Val Ser Asp Pro
355 360 365
Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys Thr Ser
370 375 380
<210> 70
<211> 574
<212> БЕЛОК
<213> Bacillus sp.
<400> 70
Met Lys Gly Leu Ile Ile Met Thr Gln Asn Thr Leu Cys Val Arg Ala
1 5 10 15
Leu Pro Ser Phe Ile Asp Glu Phe Asn Gly Ile Phe Gly Phe Gly Ser
20 25 30
Asn Ile Ala Ser Ile Ile Glu Thr Ile Phe Gly Thr Glu Val Gly Asn
35 40 45
Asp Asn Val Ile Leu Glu Glu Ile Leu Lys Asn Gln Glu Leu Leu Asn
50 55 60
Ile Ile Asn Glu Asn Ile Ser Glu Ile Asn Gly Ser Leu Asn Glu Ile
65 70 75 80
Ile Ala Asn Gly Asn Ala Asn Ser Glu Leu Leu Asn Asp Leu Ile Asn
85 90 95
Ile Ser Asn Ala Gln Gly Thr Val Leu Thr Asn Ile Gln Asp Gln Ile
100 105 110
Ser Ser Ile Asn Ser Thr Leu Asn Tyr Tyr Leu Gly Lys Met Thr Gln
115 120 125
Met Leu Asn Thr Val Ile Gln Gln Asn Ser Val Ile Ile Leu Gln Ile
130 135 140
Gln Tyr Leu Ser Lys Gln Leu Glu Glu Ile Ser Lys Lys Leu Asp Thr
145 150 155 160
Ile Asn Ile Asn Val Leu Val Asn Ser Thr Leu Thr Glu Ile Ser Pro
165 170 175
Ser Tyr Gln Arg Met Lys Tyr Val Asn Asn Lys Phe Asp Glu Leu Ser
180 185 190
Ile Thr His Thr Asp Lys Phe Ser Gln Asn Gly Lys Asn Ser Ser Asn
195 200 205
Val Glu Ile Leu Glu Thr Ala Thr Pro Thr Asp Leu Thr Gly Ile Ile
210 215 220
Ser Leu Ile Glu Phe Ala Lys Ser Val Thr Lys Asn Asp Met Asn Ser
225 230 235 240
Phe Glu Phe Tyr Leu Lys Thr Phe His Asp Val Met Val Gly Lys Thr
245 250 255
Ile Phe Glu Arg Ser Ala Leu Gln Thr Ala Arg Asp Leu Ile Lys Glu
260 265 270
Ser Asp Ile Gln Ala Asp Gly Ser Glu Val Gly Lys Thr Tyr Asn Phe
275 280 285
Leu Ile Val Leu Ser Ser Leu Leu Cys Lys Ser Tyr Ala Thr Ile Val
290 295 300
Ala Cys Arg Lys Ile Leu Cys Leu Pro Asn Ile Asp Tyr Thr Pro Ile
305 310 315 320
Ile Asn Gln Cys Ile Gln Glu Gln Thr Lys Glu Phe Lys Glu Asn Val
325 330 335
Ile Pro Tyr Leu Ser Ser Thr Phe Thr Thr Phe Asn His Ile Asp Leu
340 345 350
Gly Lys Gly Ala Ala Trp Arg Glu Ala His Ile Cys Asp Val Lys Ala
355 360 365
Ser Pro Gly Tyr Ala Leu Val Ala Phe Glu Val Val Gln Asn Gly Asn
370 375 380
Asp Phe Asp Leu Ile Gly Tyr Gln Ala Lys Leu Lys Ser Asn Tyr Gly
385 390 395 400
Ile Asp Lys Glu Ser Val Ser Lys Val Ile Thr Pro Asn Ile Asn Lys
405 410 415
Ile Ile Gly Pro Arg Ser Gly Glu Lys Lys Pro Gly Glu Glu Glu Thr
420 425 430
Ile Ser Lys His Ile Tyr Phe Asn Ser Glu Glu Val Ile Phe Pro Glu
435 440 445
Gly Tyr Val Ile Thr Asn Ile Lys Phe Lys Ser Gly Thr Leu Glu Met
450 455 460
Gly Phe His Tyr Glu Val Thr Ala Cys Leu Tyr Asp Pro Ile Thr Gly
465 470 475 480
Glu Ile Asp Thr Asn Glu Thr Leu Thr Glu Met Arg Asp Ala Asn Pro
485 490 495
Glu Thr Glu Ile Ser Pro Thr Ser Asp Asn Gly Val Tyr Phe Pro Asn
500 505 510
Arg Ile Ser Asp Ile Phe Phe Ser Pro Met Arg Ser Phe Gln Ile Asn
515 520 525
Gly Asp Pro Asn Ser His Lys Phe Ser Ile Leu Ser Thr Ser Tyr Leu
530 535 540
Ser Glu Tyr Leu Val Glu Ser Asp Leu Ile Asn Lys Glu Thr Ser Leu
545 550 555 560
Ile Phe Leu Thr Lys Asp Ile Thr Ala Asn Thr Leu Asn Asn
565 570
<210> 71
<211> 377
<212> БЕЛОК
<213> Bacillus sp.
<400> 71
Met Leu Asn Lys Ile Pro Tyr Lys Ile Leu Ala Val Ser Thr Leu Leu
1 5 10 15
Thr Ile Thr Thr Ala Asn Val Val Thr Pro Val Thr Ala Phe Ala Ser
20 25 30
Glu Ile Glu Gln Thr Thr Thr Ser Asp Met Ser Leu Ser Val Asn Glu
35 40 45
Glu Thr Met Lys Lys Ala Leu Gln Asp Ala Gly Leu Phe Ala Lys Ser
50 55 60
Met Asn Val Tyr Ser Tyr Leu Leu Ile Asn Asn Pro Asn Val Asn Phe
65 70 75 80
Glu Arg Ile Asp Val Glu Gly Asn Thr Asn Leu Pro Asn Gln Ile Val
85 90 95
Gln Asp Gln Lys Asn Ala Arg Glu His Ala Val Lys Trp Asp Thr Gln
100 105 110
Ile Lys Arg Gln Leu Leu Asn Thr Leu Thr Gly Ile Val Gln Tyr Asp
115 120 125
Thr Thr Phe Asp Asn Tyr Tyr Glu Val Leu Val Asp Ala Met Asn Asp
130 135 140
Gly Asp Gly Asp Thr Leu Lys Glu Gly Ile Thr Asp Leu Gln Gly Glu
145 150 155 160
Ile His Gln Asn Arg Glu His Thr Gln Lys Leu Ile Gln Glu Leu Thr
165 170 175
Lys Leu Lys Asp Asp Ile Gly Gln Asp Val Arg Ala Phe Gly Asp His
180 185 190
Thr Asp Leu Leu Gln Ser Ile Leu Lys Lys Gln Ala Ala Glu Ile Glu
195 200 205
Glu Asp Gln Lys Arg Leu Asp Glu Val Leu Lys Ser Val Asn Tyr Tyr
210 215 220
Lys Lys Leu Glu Ser Asp Gly Phe Asn Val Met Lys Gly Thr Val Leu
225 230 235 240
Gly Leu Pro Leu Ile Gly Gly Ile Ile Val Val Val Ala Lys Asp Asn
245 250 255
Leu Gly Lys Leu Glu Pro Val Ser Gln Gln Leu Leu Glu Lys Leu Ser
260 265 270
Gln Pro Val Asp His Lys Val Thr Leu Asn Arg Val Val Gly Val Thr
275 280 285
Tyr Asn Asn Ile Thr Glu Met His Asn Ala Ile Asp Thr Ala Ile Asn
290 295 300
Ala Leu Thr Tyr Met Ser Ala Gln Trp Asn Asp Leu Asp Ser Lys Tyr
305 310 315 320
Thr Ala Val Leu Gln His Ile Glu Asn Ala Ser Lys Lys Asp Asp Pro
325 330 335
Asn Lys Phe Lys Phe Leu Glu Pro Asn Leu Lys Ala Ala Lys Asp Ser
340 345 350
Trp Lys Thr Phe Lys Thr Asp Ala Ile Thr Leu Lys Glu Gly Ile Lys
355 360 365
Glu Leu Lys Val Glu Pro Leu Gln Lys
370 375
<210> 72
<211> 427
<212> БЕЛОК
<213> Pseudomonas sp.
<400> 72
Val Phe Leu Ile Asn Gly Ala Val Ile Phe Leu Asn Arg Ile Tyr Met
1 5 10 15
Glu Ile Asn Met Arg Gln Asp Leu Gly Lys Pro Ile Arg Ala Val Ala
20 25 30
Tyr Glu Ala Val Ala Glu Leu Glu Gly Ile Arg Thr Val Ser Glu Ala
35 40 45
Ala Lys Tyr Val Pro Val Gln Tyr Phe Gly Val Met Ala Gly Lys Asp
50 55 60
Ala Pro Asp Tyr Gln Asp Gly Thr Phe Ile Val Lys Lys Ser Asn Ile
65 70 75 80
Val Ala Ile Lys Arg Tyr Val Asn Ser Ser Leu Arg Leu Pro Val Asp
85 90 95
Leu Thr Leu Val Glu Arg Met Leu Gly Tyr Thr Arg Val Asp Ser Glu
100 105 110
Gly Leu Glu Pro Arg Asp Ile Gln Ser Leu His Gln Glu Ile His Lys
115 120 125
His Ala Leu Ser Trp Gly Thr Leu Glu Arg Glu Thr Lys Asn Leu Gly
130 135 140
Gly Arg Leu Asp Leu Phe Ser Lys Gly Phe Ile Asn Thr Gly Glu Ile
145 150 155 160
Val Ile Asp His Leu Lys Ser Phe Thr Gly Tyr Arg Asn Leu Leu Gly
165 170 175
Thr Ile Asp Thr Leu Thr Glu Lys Glu Met Glu Ala Leu Ala Ala Ile
180 185 190
Pro Leu Gly Asn Ser Asp Thr Glu Lys Val Gln Ser Leu Ala Arg Leu
195 200 205
Phe Thr Arg Met Lys Ala Asp Ile Glu Ser Phe Tyr Asn Arg Val Ile
210 215 220
Ala Val Lys Ala Leu Ala Ser Glu Phe Ser Arg Lys Ile Thr Glu Asp
225 230 235 240
Leu Leu Pro Val Ile Lys Val Lys Met Arg Asn Ala Glu Met Ala Gly
245 250 255
Ile Glu Lys Asp Ala Arg Val Glu Lys Ile Lys Phe Glu Leu Glu Glu
260 265 270
Leu Asp Gln Val Val Ala Glu Lys Leu Glu Glu Tyr Arg Ser Leu Val
275 280 285
Gly Tyr Ser Phe Thr Gly Leu Val Phe Gly Pro Ile Gly Val Ala Ile
290 295 300
Thr Gly Gly Ile Phe Gly Ser Gln Ala Glu Arg Val Arg Gly Glu Lys
305 310 315 320
Asn Ala Leu Leu Glu Arg Arg Thr Glu Leu Leu Glu Gln Met Arg Gly
325 330 335
Ala Asn Met Ser Lys Leu Leu Asn Glu Leu Ala Gly Gly Leu Val Asp
340 345 350
Ile Lys Thr Leu Val Glu Asp Ala Glu Lys Gly Ala Lys Asn Leu Glu
355 360 365
Asp Val Trp Ala Phe Ile Trp Leu Tyr Ile Asp Glu Ser Ser Ala Arg
370 375 380
Leu Ser Glu Ala Gln Thr Ala Leu Asp Leu Asn Met Leu Val Leu Asp
385 390 395 400
Leu Gln Ser Val Ile Asp Pro Trp Arg Val Ile His Gly His Ala Asn
405 410 415
Ala Leu Ser Arg Val Phe Asn Glu Val Ala Asp
420 425
<210> 73
<211> 373
<212> БЕЛОК
<213> Bacillus sp.
<400> 73
Val Ala Lys Ser Met Thr Phe Lys Val Gly Thr Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Ala Gly Asn Arg Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Glu Lys Ala Asp Ser
35 40 45
Glu Arg Phe Phe Leu His Lys Leu Asp Asp Asp Tyr Tyr Ala Met Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Ile Ser Gly Asn Glu Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ala Ser Ser
85 90 95
Gln Tyr Trp Lys Phe Ile Pro Glu Thr Glu Gly Tyr Tyr Val Ile Lys
100 105 110
Ser Lys Leu Ser Gly Lys Val Leu Asp Ile Ser Gly Asn Arg Thr Asp
115 120 125
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Glu Lys Ala Asp Ser
130 135 140
Glu Arg Phe Ala Val Glu Glu Ala Gly Ser Val Pro Leu Pro Ser Ile
145 150 155 160
Asp Lys Lys Pro Leu Ser Pro Val Pro Gln Tyr Lys Thr Ile Asn Asp
165 170 175
Gln Leu Pro Glu Glu Thr Glu His Val Val Arg Ala Phe Ala Arg Val
180 185 190
Pro Ala Ile Ser Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Gln Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys His
225 230 235 240
Thr Val Arg Thr Gly Ile Lys Val Ile Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Trp Ser Ile Gly Ala Asp Met Gly Leu Asp Phe Lys Gly
260 265 270
Phe Ser Ala Gly Met Ser Ser Gln Tyr Ser Glu Gln Leu Glu Thr Thr
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Ile Met Asp His Glu
290 295 300
Ile Arg Asn Thr Thr Thr Asp Asn Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Val Thr Glu Tyr Ser Val Arg Arg Gln Asp Gly Ser Thr Val Asn Ser
325 330 335
Ser Trp Thr Met Thr Asp Lys Asn Asn Ala His Ala Val Tyr Phe Pro
340 345 350
Lys Thr Ile Gly Asn Leu Leu Asn Glu Ser Thr Lys Gln Leu Ser Lys
355 360 365
Thr Glu Asn Val Lys
370
<210> 74
<211> 369
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 74
Met Thr Phe Lys Val Gly Thr Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Ala Gly Asn Arg Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Tyr Leu Glu Lys Ala Asp Ser Glu Arg Phe Phe
35 40 45
Leu His Lys Leu Asp Asp Asp Tyr Tyr Ala Met Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ala Ser Ser Gln Tyr Trp Lys
85 90 95
Phe Ile Pro Glu Thr Glu Gly Tyr Tyr Val Ile Lys Ser Lys Leu Ser
100 105 110
Gly Lys Val Leu Asp Ile Ser Gly Asn Arg Thr Asp Asn Asn Ala Asn
115 120 125
Val Gln Gln Tyr Glu Tyr Leu Glu Lys Ala Asp Ser Glu Arg Phe Ala
130 135 140
Val Glu Glu Ala Gly Ser Val Pro Leu Pro Ser Ile Asp Lys Lys Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Lys Thr Ile Asn Asp Gln Leu Pro Glu
165 170 175
Glu Thr Glu His Val Val Arg Ala Phe Ala Arg Val Pro Ala Ile Ser
180 185 190
Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Gln
210 215 220
Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys His Thr Val Arg Thr
225 230 235 240
Gly Ile Lys Val Ile Asp Gln Glu Thr Ala Thr Lys Thr Val Ser Trp
245 250 255
Ser Ile Gly Ala Asp Met Gly Leu Asp Phe Lys Gly Phe Ser Ala Gly
260 265 270
Met Ser Ser Gln Tyr Ser Glu Gln Leu Glu Thr Thr Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Ile Met Asp His Glu Ile Arg Asn Thr
290 295 300
Thr Thr Asp Asn Met Ala Tyr Ser Arg Tyr Ile Leu Val Thr Glu Tyr
305 310 315 320
Ser Val Arg Arg Gln Asp Gly Ser Thr Val Asn Ser Ser Trp Thr Met
325 330 335
Thr Asp Lys Asn Asn Ala His Ala Val Tyr Phe Pro Lys Thr Ile Gly
340 345 350
Asn Leu Leu Asn Glu Ser Thr Lys Gln Leu Ser Lys Thr Glu Asn Val
355 360 365
Lys
<210> 75
<211> 411
<212> БЕЛОК
<213> Bacillus sp.
<400> 75
Leu Ser Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys Lys
1 5 10 15
Val Met Ala Gly Leu Leu Val Gly Ala Thr Ser Leu Ser Ile Trp Ala
20 25 30
Pro Ile Ser Glu Ala Ala Ala Pro Glu Asn Asn Arg Tyr Tyr Asn Ile
35 40 45
Ala Leu Lys Ser Asn Thr Lys Lys Val Trp Asn Val Ser Gln Gly Ser
50 55 60
Asn Asp Asn Asp Arg Ala Ile Val Leu Trp Gln Gly Gly Ser Ala Asp
65 70 75 80
His Glu Arg Phe Ala Phe Phe Gln Leu Asp Gly Gly Ala Tyr Ala Ile
85 90 95
Val Asn Lys Asn Ser Gly Lys Val Val Thr Phe Gly Thr Gly Ser Trp
100 105 110
Val Ser Gly Gly Pro Ile Pro Asp Ser Glu Thr Leu Gln Gln Gln Lys
115 120 125
Trp Thr Gly Glu Ala Lys Gln Lys Trp Asn Leu Arg Asp Leu Gly Ser
130 135 140
Asp Gly His Gly Ser Asn Asn Tyr Glu Ile Val Asn Gln Ala Asn Gly
145 150 155 160
Lys Val Pro Ser Tyr Arg Trp Ile Gly Ser Thr Val Asp Asn Thr Glu
165 170 175
Tyr Leu Lys Leu Asp Asn Ser Asn Pro Ser Asp Ala Asp Lys Val Phe
180 185 190
Thr Leu Phe Asn Gly Thr Met Asn Leu Trp Gly Gln Asn Ile Thr Phe
195 200 205
Phe Asn Asp Thr Ile Ser Val Pro Lys Leu Pro Ala Thr Gly Thr Arg
210 215 220
Pro Asp Ala Pro Asn Tyr Thr Gly Gly Val Asp Gln Gln Leu Pro Gln
225 230 235 240
Thr Ser Asn Ser Val Val Val Gly Ala Thr Leu Ile Pro Cys Phe Met
245 250 255
Val Asn Asp Asn Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro
260 265 270
Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ala
275 280 285
Val Ile Pro Ala Gly Leu Thr Arg Asn Tyr Ser Phe Lys Thr Gly Met
290 295 300
Thr Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Ile
305 310 315 320
Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys
325 330 335
Thr Glu Ile Ser Lys Thr Ile Gln Thr Glu Ile Ser Thr Thr Asn Thr
340 345 350
Glu Ala Ser Glu Glu Thr Ile Thr Ser Thr Val Thr Ser Glu Ala Gly
355 360 365
Lys Thr Thr Gly Tyr Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu
370 375 380
Lys Arg Ala Asp Gly Ser Ile Val Ser Asp Pro Trp Val Val Lys Asn
385 390 395 400
Asn Lys Ile Thr Ile Ala Arg Lys Asn Ala Gln
405 410
<210> 76
<211> 387
<212> БЕЛОК
<213> Bacillus sp.
<400> 76
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ala Ser Gln Ala Ala Pro Ala Asn
20 25 30
Asn Ala Tyr His Ser Ile His Leu Ala Ala Asp Ala Thr Leu Lys Trp
35 40 45
Asp Val Tyr Gly Asn Ser Pro Asn Asp Glu Ala Gly Ile Leu Leu Tyr
50 55 60
Asn Gly Asn Phe Ser Asp Asn Glu Gln Phe Leu Phe Phe Pro Leu Asp
65 70 75 80
Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Ala Val Thr
85 90 95
Ile Gly Ala Gly Phe Ile Thr Pro Thr Asp Tyr Ser Tyr Asn Arg Glu
100 105 110
Gly Leu Lys Gln Ser Lys Trp Thr Gly Ala Ala Ala Asp Lys Trp Tyr
115 120 125
Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly Tyr
130 135 140
Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile Ser Gly Gln Lys Tyr
145 150 155 160
Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn Asp Arg Leu Phe His
165 170 175
Ile Pro Ala Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu Pro Thr
180 185 190
Ile Gly Thr Arg Pro Asp Ala Pro Asp Tyr Arg Glu Asp Phe Pro Gly
195 200 205
Arg Ile Thr Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly
210 215 220
Ala Ser Leu Ile Pro Cys Ile Met Val Asn Asp Asn Gln Ala Ser Asp
225 230 235 240
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu
245 250 255
Tyr Trp Asp Lys Thr Phe Ser Thr Val Leu Pro Ala Gly Tyr Thr Lys
260 265 270
Asn Tyr Ser Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln Arg Met
275 280 285
Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe
290 295 300
Gly Asp Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr Ile Gln
305 310 315 320
Thr Glu Ile Thr Ser Thr Ser Thr Glu Ala Ser Glu Glu Thr Ile Thr
325 330 335
His Asn Val Thr Ser Glu Ala Gly Lys Thr Thr Ala Phe Thr Glu Tyr
340 345 350
Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val
355 360 365
Ser Asp Pro Trp Val Val Lys Asn Asn Lys Asn Thr Ile Val Arg Lys
370 375 380
Ile Glu Lys
385
<210> 77
<211> 364
<212> БЕЛОК
<213> Bacillus sp.
<400> 77
Val Arg Ser Leu Tyr Ile Asn Phe Tyr Lys Lys Cys Phe Leu Ser Ile
1 5 10 15
Met Ile Ala Gly Val Gly Met Ser Asn Val Ile Pro Leu Lys Pro Leu
20 25 30
Ala Glu Gly Phe Glu Glu Gly Gln Gln Leu Gln Gln Lys Ser Ala Glu
35 40 45
Ser Tyr Ser Leu Gly Pro Ala Gly Phe Lys Asp Val Met Ala Gln Thr
50 55 60
Thr Thr Ser Val Phe Ala Met Asp Ser Tyr Ala Lys Leu Ile Arg Asn
65 70 75 80
Gln Gln Glu Thr Asp Leu Asn Lys Ile Ser Ser Ile Asn Asp Asn Leu
85 90 95
Arg Leu Lys Met Ile Gln His Gln Lys Asp Ala Lys Ile Asn Ala Ala
100 105 110
His Trp Leu Glu Lys Met Lys Pro Gln Ile Met Lys Thr Asn Gln Asn
115 120 125
Ile Ile Asn Tyr Asn Asp Val Phe Gln Thr Asn Tyr Asn Ser Leu Leu
130 135 140
Lys Ala Ile Asp Gln Lys Asp Ser Gly Lys Leu Lys Glu Gly Leu Glu
145 150 155 160
Glu Leu Tyr Ala Ser Ile Leu Lys Asn Glu Lys Glu Val Asp Val Leu
165 170 175
Leu Gly Asp Leu Lys Thr Phe Arg Asn Arg Met Glu Arg Asp Thr Thr
180 185 190
Ser Phe Lys Asn Asp Ser Asn Gln Leu Asn Ser Ile Leu Ala Ser Thr
195 200 205
Asn Ala Gly Ile Pro Val Leu Gln Gln Gln Ile Asn Ala Tyr Asn Asp
210 215 220
Leu Ile Lys Lys Ser Asn Asn Met Val Ile Thr Gly Gly Ile Leu Cys
225 230 235 240
Ser Ser Leu Leu Ala Cys Ile Ala Gly Gly Pro Met Ile Ala Asn Ala
245 250 255
Leu Asn Glu Ile Ala Arg Ala Glu Arg Glu Ile Ala Asn Leu Lys Glu
260 265 270
Lys Ile Ser Gly Ala Gln Lys Asp Val Val Ile Leu Thr Asp Ile Asn
275 280 285
Ile Lys Thr Ala Asn Met Thr Asn Thr Ile Asp Ala Ala Ile Thr Ala
290 295 300
Leu Gln Lys Ile Ser Asn Gln Trp His Thr Val Gly Ala Lys Tyr Asn
305 310 315 320
Asn Leu Leu Lys Asn Val Lys Asp Ile Ser Pro Glu Glu Phe Ala Phe
325 330 335
Ile Lys Glu Asp Leu Asn Thr Ala Arg Asp Ser Trp Lys Asp Ile Lys
340 345 350
Asp Tyr Thr Glu Lys Leu Asn Glu Gly Val Ser Lys
355 360
<210> 78
<211> 349
<212> БЕЛОК
<213> Brevibacillus sp.
<400> 78
Met Asp Ile Lys Lys Asp Thr Tyr Tyr Lys Ile Ile Asn Arg Asn Ser
1 5 10 15
Gly Leu Val Ile Asp Val Gln Ala Lys Ser Thr Gly Asn Ser Ala Leu
20 25 30
Leu Glu Gln Leu His Trp Leu Gly Glu Ser Phe Gln Gln Trp Leu Val
35 40 45
Phe Gln Leu Asp Gly Gly Leu Ser Ala Ile Val Asn Arg Asn Ser Gly
50 55 60
Leu Val Ile Asp Val Gln Ala Lys Ser Thr Ser Asn Ser Ala Leu Leu
65 70 75 80
Glu Gln Leu His Trp Leu Gly Glu Ser Phe Gln Gln Trp Ile Leu Glu
85 90 95
Lys Val Asp Gly Tyr Phe Lys Ile Lys Asn Gln Asn Ser Gly Leu Val
100 105 110
Ile Asp Val Gln Ala Lys Ser Thr Ser Asn Ser Ala Leu Leu Glu Gln
115 120 125
Leu His Trp Leu Gly Glu Ser Phe Gln Gln Trp Ser Phe Glu Glu Val
130 135 140
Glu Thr Ile Lys Leu Pro Ser Ile Ala Thr Gln Glu Leu Pro Pro Pro
145 150 155 160
Pro Glu Tyr Lys Ser Leu Asp Asp Asn Leu Pro Asp Ser Thr Asp Pro
165 170 175
Val Ile Thr Ser Tyr Thr Leu Ile Pro Cys Ile Met Val Glu Asp Asn
180 185 190
Gly Trp Ser Ser Lys Ser Lys Ile Thr Glu Ser Pro Tyr Tyr Met Leu
195 200 205
Thr Lys Thr Gln Tyr Trp Lys Lys Ile Asp Ser His Thr Phe Ala Pro
210 215 220
Lys Thr Gln Tyr Thr Ser Lys Thr Thr Tyr Gly Met Ser Glu Met Asp
225 230 235 240
Gln Glu Ser Met Thr Tyr Thr Thr Gly Ile Thr Val Thr Ala Asp Ala
245 250 255
Gly Phe Ser Phe Lys Lys Val Ser Ser Ser Ile Ala Ala Ser Val Thr
260 265 270
Lys Glu Leu Gln Val Gln Arg Ser Thr Thr Thr Gln Ile Met Thr Ile
275 280 285
Gln Glu Asn Thr Ile Ser Ile Ser Asn Asp Asn Asn Tyr Lys Ile Ala
290 295 300
Trp Ser Leu Tyr Ala Leu Val Ser Glu Tyr His Leu Lys Arg Ala Asn
305 310 315 320
Gly Thr Ser Val Thr Ser Ser Trp Thr Val Ile Asn Lys Asn Asp Glu
325 330 335
Arg Glu Ser Tyr Tyr Pro Tyr Asp Ala Asn Leu Lys Gln
340 345
<210> 79
<211> 288
<212> БЕЛОК
<213> Bacillus sp.
<400> 79
Met Met Ser Asn Glu Ser Lys Asn Asn Glu Asn Met Asn Leu Pro Ile
1 5 10 15
Ile Asn Asp Glu Asp Ser Ile Pro Ser Glu Ile Arg Asp Ser Arg Ser
20 25 30
Lys Ile Thr Phe Pro Asp Phe Asn Ile Tyr Lys Ile Gly Thr Phe Cys
35 40 45
Gly Lys Tyr Val Asp Ile Pro Gly Gly Ala Asn Glu Asp Ser Ser Ala
50 55 60
Ile Gln Phe Gln Thr Gln Asp Gly Asp Asn Gln Lys Phe Leu Ile Phe
65 70 75 80
Thr Leu Asp Asp Ser Tyr Ser Ile Ile Ala Ala Arg His Ser Gly Lys
85 90 95
Val Met Asp Ala Tyr Tyr Asn Ser Ser Gln Asn Arg Ile Ile Gln Phe
100 105 110
Asp Phe His Asn Thr Asn Asn Gln Lys Phe Phe Leu Ser Asp Asn Gly
115 120 125
Thr Ile Ala Ser Lys Gln Asp Arg Gln Val Trp Asp Val Gln Gly Glu
130 135 140
Ser Thr Gln Asn Gly Ala Arg Ile Val Val Phe Lys Phe Ile Asp Val
145 150 155 160
Arg Asn Gln Lys Phe Thr Leu Asn Lys Leu Gly Ser Val Pro Val His
165 170 175
Gln Pro Thr Leu Ser Pro Leu Pro Ser Ala Pro Asp Phe Arg Thr Asn
180 185 190
Asp Ile Asn Glu Gln Leu Pro Asp Gln Thr Asn Pro Val Asn Thr His
195 200 205
Phe Thr Tyr Leu Pro Tyr Phe Met Val Lys Asp Pro Leu Tyr Asn Ala
210 215 220
Gln Gln Gln Ile Lys Asn Ser Pro Tyr Tyr Thr Leu Val Arg Arg Gln
225 230 235 240
Tyr Trp Glu Lys Lys Thr Gln Arg Ile Leu Ala Pro Ser Asp Thr Tyr
245 250 255
Glu Tyr Ser Glu Thr Ile Gly Val Ser Arg Thr Asp Gln Ser Ser Met
260 265 270
Thr Asp Thr Thr Glu Ile Ser Asn Arg Cys Arg Phe Arg Ile Cys Ile
275 280 285
<210> 80
<211> 596
<212> БЕЛОК
<213> Bacillus sp.
<400> 80
Met Lys Tyr Lys Asp Arg Thr His Ala Lys Arg Lys Tyr Lys Gln Ala
1 5 10 15
Leu Leu Val Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Ser Gly Ala Ser Ala Phe Ala Glu Glu Asn Ser Ile Ser Leu Gln Gln
35 40 45
Val Thr Gly Ala Gln Gln Met Gln Ala Ser Thr Tyr Ser Ala Gly Gly
50 55 60
Ser Thr Ser Gln Thr Ala Glu Ser Thr Ser Thr Leu Lys Ser Leu Leu
65 70 75 80
Thr Met Asp Asn Leu Lys Lys Val Gly Thr Leu Thr Gly Asp Val Leu
85 90 95
Lys Thr Ala Tyr Lys Asp Ala His Glu Asn Asn Gly Asn Tyr Asn Asn
100 105 110
Thr Phe Arg Asp Leu Ala Met Gly Gly Thr Ser Leu Ile Pro Tyr Gly
115 120 125
Gly Val Leu Val Ser Gly Leu Ile Gly Leu Ile Trp Pro Val Asn Thr
130 135 140
Asp Ala Gln Lys Asn Gln Met Gln Lys Leu Ile Asp Asn Leu Asn Asn
145 150 155 160
Met Met Asp Ser Lys Ile Thr Ala Asn Glu Met Ala Asn Ile Gln Ser
165 170 175
Glu Val Asn Thr Leu Lys Glu Arg Leu Gln Arg Phe Glu Lys Asn Ile
180 185 190
Asn Met Ser Ile Ser Arg Ser Val Tyr Arg Leu Ala Thr Asp Lys Glu
195 200 205
Gln Ala Leu Ala Thr Glu Ala Ser Ala Ile Ser Asn Asp Phe Ser Lys
210 215 220
Ile Leu Gly His Ser Lys Gln Glu Leu Gln Thr Ile Pro Ala Leu Pro
225 230 235 240
Val Tyr Ala Gln Leu Ala Thr Ala His Ile Glu Phe Leu Gln Phe Leu
245 250 255
Ala Lys Asn Gly Thr Gly Ser Lys Leu Gln Tyr Thr Thr Ala Ala Leu
260 265 270
Asn Thr Leu Leu Tyr Gly Glu Pro Asp Glu Thr Phe Glu Gln Arg Met
275 280 285
His Lys Leu Ala Glu Lys Tyr Arg Glu His Val Asn Thr Thr Tyr Lys
290 295 300
Leu Ala Asp Glu Lys Met Asn Val Lys Ile Arg Asp Ile Ala Asn Asn
305 310 315 320
Ser Pro Val His His Met Asn Asn Tyr Glu Thr Val Asp Glu Met Met
325 330 335
Gly Ala Met Gln Val Tyr Met Leu Asn Met His Pro Ala Ala Asn Asn
340 345 350
Tyr Asp Ser Met Lys Lys Lys Leu Asn Glu Asp Val Thr Ala Leu Leu
355 360 365
Asp Leu Lys Thr Lys Lys Gln Glu Leu Leu Ala Ser Thr Val Gly Asn
370 375 380
Glu Ala Phe Gln Lys Ile Val Ile Gly Glu Trp Asp Ala His Asn His
385 390 395 400
Val Ala Asp Leu Asn His Ile Gln Gln Thr Gly Trp Ala Tyr Leu Lys
405 410 415
Asn Asn Trp Tyr Tyr Leu Ser Pro Asp Asp Ser Thr Lys Asn Leu His
420 425 430
Gly Asp Thr Phe Gln Arg Gly Gln Gln Val Thr Gly Gln Ile Glu Ile
435 440 445
Asp His Lys Thr Tyr Phe Phe Lys Pro Asp Thr Gly Gln Met Val Thr
450 455 460
Arg Trp Leu Gln Ile Gly Asp Lys Phe Phe Tyr Ile Ser Pro Tyr Asp
465 470 475 480
Gly Phe Lys Asn Trp Asp Gly Val Glu Phe Lys Gln Gly Glu Leu Val
485 490 495
Thr Gly Trp Leu Glu Leu Gly Asp Arg Ala Thr Tyr Tyr Leu Ser Pro
500 505 510
Glu Asn Asp Lys Lys Asn Asp Glu Asn Lys Asn Ser Lys Thr Phe Asp
515 520 525
Lys Gly Gln Met Met Thr Gly Trp Ile Leu Val Lys Asn Val Ser Tyr
530 535 540
Tyr Met Asp Asn Lys Glu Gly Asn Lys Asp Phe Gln Asp Val Lys Gly
545 550 555 560
Arg Met Leu Arg Gly Thr Asn Lys Gly Lys Gly Phe Arg Leu Asn Ser
565 570 575
Gly Lys Ser Pro Asp Asp Phe Lys Thr His Lys Phe Asn Asp Asn Gly
580 585 590
Thr Trp Ile Asn
595
<210> 81
<211> 386
<212> БЕЛОК
<213> Bacillus sp.
<400> 81
Met Lys Phe Leu Ser Lys Lys Val Ile Thr Gly Leu Phe Ala Gly Thr
1 5 10 15
Met Ala Leu Ser Ile Gly Ala Pro Ala Ser Gln Ala Ala Thr Pro Glu
20 25 30
Lys Asn Gly Tyr Tyr Ser Ile His Leu Ala Ser Asn Ala Trp Lys Lys
35 40 45
Trp Asp Val Val Gln Ser Ser Leu Asp Asn Glu Arg Gly Ile Leu Leu
50 55 60
Tyr Asp Val Ser Gly Arg Ile Pro Asp Asn Gln Gln Phe Ala Phe Phe
65 70 75 80
Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys
85 90 95
Pro Val Thr Ile Gly Asp Asn Val Leu Pro Met Ala Gly Leu Gly Gly
100 105 110
Asn Ser Gly Ala Leu Lys Gln Asn Asn Trp Thr Gly Asn Ala Asn Glu
115 120 125
Lys Trp Tyr Leu Arg Asp Leu Gly Ser Asn Asp Phe Glu Ile Val Asn
130 135 140
Gln Gly Thr Gly Lys Val Ala Ser Tyr Ala Trp Val Gly Asp Leu Val
145 150 155 160
Arg Asn Tyr Glu Tyr Val Asp Leu Asp Asn Ala Asn Pro Ser Asp Lys
165 170 175
Asp Arg Val Phe His Ile Pro Ala Ala Arg Ser Thr Phe Thr Val Pro
180 185 190
Thr Leu Pro Ala Thr Gly Thr Arg Pro Val Ala Pro Asn Tyr Thr Gly
195 200 205
Gly Ile Asp Glu Gln Leu Pro Gln Thr Ser Glu Ser Val Ile Val Gly
210 215 220
Ala Ser Leu Ile Pro Cys Ile Met Val Lys Asp Ser Gln Thr Asn Asp
225 230 235 240
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu
245 250 255
Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu
260 265 270
Ala Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met
275 280 285
Thr Asp Thr Leu Ser Met Lys Phe Gly Ala Asp Phe Gly Ile Lys Phe
290 295 300
Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln
305 310 315 320
Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser
325 330 335
Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr
340 345 350
Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val
355 360 365
Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys
370 375 380
Thr Ser
385
<210> 82
<211> 432
<212> БЕЛОК
<213> Bacillus sp.
<400> 82
Leu Val Leu Ser Phe Tyr Ile Ala Lys Val Ile Leu Phe Arg Asn Lys
1 5 10 15
Ser Ile Ser Asn Val Gln Tyr Asn Phe Ile Phe Cys Asn Leu Val Gly
20 25 30
Ile Leu Pro Ala Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys
35 40 45
Lys Val Met Ala Gly Leu Gly Val Gly Ala Met Ser Leu Ser Ile Trp
50 55 60
Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala Asn Asn Glu Tyr His Thr
65 70 75 80
Ile His Leu Ala Ala Asn Thr Asn Ile Ala Trp Asp Val Tyr Lys Gly
85 90 95
Trp Gln Ser Asp Asp Ala Gly Ile Val Leu Trp Asp Gly Ala Arg Gly
100 105 110
Asp Asn Glu Gln Phe Val Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala
115 120 125
Ile Val Asn Lys Asn Ser Gly Lys Pro Val Gly Ile Gly Ser Ala Ile
130 135 140
Ile Gly Ser Asp Gly Val Arg Asn Phe Pro Ser Phe Asn Asn Glu Gly
145 150 155 160
Leu Arg Gln Thr Ser Trp Thr Gly Ala Pro Asn Glu Gln Trp Tyr Leu
165 170 175
Arg Asp Lys Gly Asn Asn Asn Phe Glu Ile Val Asn Gln Gly Ser Gly
180 185 190
Lys Val Ala Ser Trp Ala Gly Val Glu Met Tyr Gly Tyr Lys Asp Tyr
195 200 205
Val Asp Leu Asp Glu Ser Asn Pro Ser Asp Lys Asp Arg Leu Phe Asn
210 215 220
Ile Pro Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu Pro Ala Val
225 230 235 240
Gly Thr Arg Pro Asn Ala Pro Asp Tyr Asn Thr Ser Gly Ala Ile Asp
245 250 255
Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu
260 265 270
Ile Pro Ser Ile Met Val Lys Asp Asn Gly Ala Ser Asp Tyr Thr Lys
275 280 285
Ile His Asn Ser Pro Tyr Tyr Val Leu Val Lys Glu Glu Tyr Trp Glu
290 295 300
Lys Val Arg Ser Glu Ile Ile Pro Ala Gly Ala Thr Ser Lys Tyr Thr
305 310 315 320
Val Lys Ser Gly Val Ser Glu Ile Asp Gln Lys Lys Met Ser Asp Thr
325 330 335
Leu Ala Met Ser Phe Gly Ser Asp Leu Gly Leu Lys Phe Gly Glu Ser
340 345 350
Ser Leu Ser Ile Lys Ser Ser Val Ser Arg Thr Ile Gln Thr Glu Val
355 360 365
Thr Lys Thr Ser Thr Asn Ala Thr Glu Glu Thr Glu Glu Lys Ser Ile
370 375 380
Pro Ser Ile Pro Gly Lys Asn Thr Gly Phe Thr Ala Tyr Gln Leu Val
385 390 395 400
Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly Ser Val Val Thr Asp Pro
405 410 415
Ser Ile Val Arg Asp Ser Lys Gln Thr Ile Ala Arg Thr Ile Ala Asn
420 425 430
<210> 83
<211> 400
<212> БЕЛОК
<213> Bacillus sp.
<400> 83
Met Tyr Ile Leu Leu Leu His Phe Arg Lys Lys Trp Tyr Ile Ile Lys
1 5 10 15
Ser Gln Lys Phe Gln Lys Gln Lys Arg Glu Lys Lys Met Lys Arg Tyr
20 25 30
Lys Lys Leu Leu Met Val Ala Pro Leu Ala Cys Met Leu Gly Thr Gly
35 40 45
Val Phe Thr Leu Pro Asn Val Ser His Ala Ala Glu Val Ala Asp Val
50 55 60
Tyr Asn Gln Gly Thr Thr Tyr Ser Lys Glu Asn Met Glu Lys Ala Leu
65 70 75 80
Tyr Leu Arg Leu Leu Glu Gly Ile Glu Arg Ser Pro Glu Leu Arg Gln
85 90 95
Lys Phe Lys Met Asn Gln Asp Glu Lys Ile Gln Arg Glu Tyr Phe Pro
100 105 110
Glu Asn Asp Pro Leu Val Gly Gly Ile Ala Gly Thr Ile Gly Thr Met
115 120 125
Gly Gly Ala Ile Gly Gly Ala Ala Leu Ala Ser Arg Val Asp Tyr Gly
130 135 140
Leu Lys Ser Gln Leu Asn Gln Val Asn Ile Thr Ser Val Gly Thr Asp
145 150 155 160
Phe Lys Val Thr Pro Ser Lys Val Val Glu Glu Asp Gln Gly Val Ile
165 170 175
Asn Leu Leu Ser Tyr Arg Asn Asn Thr Pro Glu Lys Gln Lys Ala Phe
180 185 190
Thr Thr Glu Glu Ser Tyr Lys Lys Val Asp Thr Val Thr Ala Thr Asn
195 200 205
Gln Tyr Gly Phe Lys Leu Ser Ala Lys Ser Val Thr Lys Phe Gly Phe
210 215 220
Asp Ile Gly Leu Ala Lys Gly Glu Gln Thr Phe Glu Val Ser Ala Glu
225 230 235 240
Phe Ala Phe Asn Arg Thr Asp Thr Phe Thr Thr Thr Asn Glu Lys Thr
245 250 255
Val Thr Phe Lys Ala Gln Glu Val Ile Ala Ala Ser Gly Gly Thr Thr
260 265 270
Asp Tyr Phe Ser Arg Val Lys Lys Ala Lys Phe Ser Gly Ser Phe Gln
275 280 285
Thr Asp Ala Ala Leu Asp Ser Leu Lys Val Lys Leu Pro Ile Val Lys
290 295 300
Gly Gly Asn Met Asn Val Thr His Thr Glu Glu Val Thr Leu Asn Ser
305 310 315 320
Glu Asp Leu Tyr Asn Ile Phe Lys Leu Thr Ser Lys Asp Phe Pro Leu
325 330 335
Pro Ser Tyr Leu Ser Phe Asp Asp Thr Asn Lys Ser Ile Leu Val Lys
340 345 350
Asn Ala Thr Phe Asn Tyr Thr Gly Glu Ile Gly Tyr His Thr Ala Ile
355 360 365
Asp Ala Lys Phe Thr Pro Phe Asp Gln Thr Lys Ala Thr Ala Val Met
370 375 380
Pro Tyr Ala Lys Tyr Ala Glu Lys Thr Gln Ala Asn Ala Leu Leu Arg
385 390 395 400
<210> 84
<211> 404
<212> БЕЛОК
<213> Bacillus sp.
<400> 84
Leu Ser Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys Lys
1 5 10 15
Val Met Ala Gly Leu Leu Val Gly Ala Thr Ser Leu Ser Ile Trp Ala
20 25 30
Pro Thr Ser Gln Ala Ala Ala Pro Glu Asn Asn Arg Tyr Tyr Ser Val
35 40 45
His Leu Ala Ala Asp Thr Ala Leu Lys Trp Asp Val Tyr Gly Asn Gly
50 55 60
Thr Tyr Asp Asp Ala Gly Ile Leu Leu Tyr Asn Gly Asn Leu Gly Asp
65 70 75 80
Asn Glu Gln Phe Val Phe Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile
85 90 95
Val Asn Lys Asn Ser Gly Lys Ala Val Thr Ile Gly Gly Gly Trp Val
100 105 110
Gly Asp Asn Ala Phe Ser Tyr Asn Arg Glu Gly Leu Ile Gln Ser Lys
115 120 125
Trp Thr Gly Ala Ala Asp Asn Lys Trp Tyr Leu Arg Asp Lys Gly Asn
130 135 140
Asn Asn Tyr Glu Ile Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr
145 150 155 160
Ala Arg Leu Gly Ile Thr Gly Pro Lys Tyr Val Asp Leu Asp Asp Ser
165 170 175
Asn Pro Ser Asp Asn Asp Arg Leu Phe Lys Val Ser Lys Ser Pro Trp
180 185 190
Leu Tyr Gly Asp Ile Pro Gly Leu Asn Leu Asp Tyr Gly Thr Phe Ser
195 200 205
Leu Pro Gln Leu Pro Ala Thr Gly Asn Arg Pro Asp Ala Pro Asn Tyr
210 215 220
Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val
225 230 235 240
Val Gly Ala Ser Leu Val Pro Cys Ile Met Val Asn Asp Ser Gln Ala
245 250 255
Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys
260 265 270
Glu Glu Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu
275 280 285
Ser Glu Ser Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln
290 295 300
Lys Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ala Asp Leu Gly Ile
305 310 315 320
Lys Phe Gly Asp Val Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr
325 330 335
Ile Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr
340 345 350
Arg Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr
355 360 365
Gln Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser
370 375 380
Thr Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr
385 390 395 400
Arg Lys Thr Ser
<210> 85
<211> 378
<212> БЕЛОК
<213> Bacillus sp.
<400> 85
Leu Met Thr Asn Glu Asn Asn Thr Asn Thr Ser Ala Ser Asn Gly Glu
1 5 10 15
Asn Leu Ile Pro Ser Glu Val Leu Ala Ser Gly Lys Gln Ile Ser Phe
20 25 30
Pro Asp Phe Asn Phe Tyr Lys Ile Arg Thr Phe Cys Gly Lys Tyr Ile
35 40 45
Asp Ile Arg Gly Gly Ser Met Asp Asp Leu Ala Tyr Ala Leu Gln Tyr
50 55 60
Thr Gly His Asn Ala Asp Asn Gln Lys Phe Leu Ile Phe Thr Leu Asp
65 70 75 80
Asn Asn Tyr Ser Val Ile Ala Ala Lys His Ser Gly Lys Val Met Asp
85 90 95
Ile Trp Asp Thr Pro Asn Phe Pro Pro Gly Leu Pro Asp Ile Leu Thr
100 105 110
Gln Tyr Tyr Phe Lys Asn Gly Asp Asn Gln Lys Phe Phe Ile Ala Asn
115 120 125
Asp Gly Ser Val Ala Val Lys Gln Asp Gly Arg Val Leu Asp Val His
130 135 140
Asp Gly Arg Thr Ser Asn Asp Val Pro Ile Ile Ala Tyr Asn Tyr Gln
145 150 155 160
Gly Ser Ala Asn Gln Lys Phe Asn Leu Glu Lys Ser Gly Thr Val Ser
165 170 175
Ile Lys Pro Pro Thr Thr Gly Thr Leu Pro Pro Ala Pro Asp Phe Lys
180 185 190
Thr Thr Asn Leu Asp Glu Glu Leu Pro Asp Asp Thr Thr Pro Val Ile
195 200 205
Thr His Ala Thr Tyr Leu Pro Tyr Phe Met Val Lys Asp Pro Tyr Tyr
210 215 220
Asn Ala Gln Gln Lys Ile Thr Asn Ser Pro Tyr Tyr Ile Leu Val Arg
225 230 235 240
Arg Gln Tyr Trp Glu Lys Lys Thr Gln Arg Val Leu Ala Pro Ser Glu
245 250 255
Thr Tyr Asp Tyr Met Glu Thr Thr Gly Val Ser Arg Thr Asp Gln Thr
260 265 270
Ser Met Thr Glu Thr Thr Glu Ile Ser Ile Gly Ala Asp Leu Gly Phe
275 280 285
Met Phe Lys Gly Phe Ser Leu Gly Leu Ser Thr Ser Ile Thr Lys Ser
290 295 300
Leu Ser Val Thr Lys Ser Thr Ser Asn Thr Glu Ser Thr Glu Ile Ser
305 310 315 320
Lys Arg Val Val Tyr Thr Asn Pro Phe Lys Tyr Gln Ile Ala Tyr Ala
325 330 335
Lys Tyr Met Leu Ile Asn Glu Tyr Tyr Val Thr Arg Ser Asn Gly Glu
340 345 350
Met Ile Thr Ser Gly Asp Ala Ser Tyr Trp Lys Val Pro Asp Pro Glu
355 360 365
Arg Thr Val Ser Arg Ile Ile Pro Arg Arg
370 375
<210> 86
<211> 373
<212> БЕЛОК
<213> Bacillus sp.
<400> 86
Val Gly Lys Ser Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Ser Gly Asn Gln Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Ala Asp Ala Pro Ser
35 40 45
Glu Arg Phe Phe Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Ile Ser Gly Asn Gln Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ala Pro Ser
85 90 95
Glu Tyr Trp Tyr Phe His Arg Glu Ala Asp Gly His Tyr Val Ile Glu
100 105 110
Ser Lys His Ser Gly Lys Val Leu Asp Ile Ala Gly Asn Ser Thr Ser
115 120 125
Asn Asn Ala Asn Val Gln Gln Tyr Glu Phe Leu Thr Asp Ala Pro Ser
130 135 140
Glu Arg Phe Ala Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Asn Thr Gln Pro Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Glu Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val
180 185 190
Pro Ala Ile Ala Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr
225 230 235 240
Thr Thr Lys Thr Gly Ile Lys Val Thr Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Leu Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly
260 265 270
Phe Ser Val Gly Met Ser Ser Gln Tyr Ser Thr Gln Leu Gln Thr Ser
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu
290 295 300
Ile Lys Asn Pro Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Ser Val Gln Arg Lys Ser Gly Thr Ile Val Asn Ser
325 330 335
Pro Trp Thr Met Thr Asp Lys Thr Arg Thr His Ala Val Thr Tyr Pro
340 345 350
Asn Ala Glu Glu Lys Ala Leu Asn Glu Asn Thr Lys Lys Leu Ser Lys
355 360 365
Thr Glu Ser Val Asn
370
<210> 87
<211> 665
<212> БЕЛОК
<213> Bacillus sp.
<400> 87
Met Lys Tyr Lys Asn Arg Lys Asp Thr Lys Arg Lys Tyr Lys Gln Ala
1 5 10 15
Leu Leu Thr Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Ser Thr Thr Ser Ala Phe Ala Glu Glu Asn Lys Ala Asn Ile Gln Lys
35 40 45
Ala Pro Ser Val Ser Ala Thr Asp Gly Pro Val Phe Lys Lys Glu Lys
50 55 60
Asp Gly Ser Phe Thr Val Leu Lys Asp Lys Ile Phe Asn Met Glu Thr
65 70 75 80
Leu Lys Ala Ile Gly Ser Leu Gly Gly Ala Gly Leu Lys Gln Ala Tyr
85 90 95
Ala Asp Ser Gln Val Asp Gly Gly Asn Phe Asn Asn Thr Phe Arg Thr
100 105 110
Leu Ala Met Gly Ser Ala Ala Leu Ile Pro Tyr Gly Gly Val Val Leu
115 120 125
Ser Pro Leu Ile Gly Leu Leu Trp Ser Glu Asp Lys Thr Ala Glu Ala
130 135 140
Asn Gln Leu Lys Lys Leu Met Glu Ser Ile Ala Ala Gln Thr His Asp
145 150 155 160
Gln Ile Ala Lys Tyr Asp Thr Ala Lys Leu Ser Gln Ala Leu Gln Asp
165 170 175
Leu Glu Glu Lys Val Ala Glu Phe Glu Lys Ile Val Pro Ile Pro Gly
180 185 190
Asn Gly Met Phe Tyr Ser Asp Gly Gln Leu Gly Asp Tyr Glu Asn Ile
195 200 205
Gln Ile Ser Ala Lys Asn Leu Asn Asn Ser Phe Arg Thr Ala Ile Ser
210 215 220
Ala Cys Gln Thr Glu Gly Tyr Lys Glu Ser Glu Leu Pro Ile Tyr Met
225 230 235 240
Ile Leu Ala Thr Ala His Met Asn Phe Met Asn Tyr Val Ile Glu Asn
245 250 255
Gly Glu Glu Leu Leu Lys Phe Lys Pro Ser Val Leu Gln Ala Glu Phe
260 265 270
Thr Asp Lys Gln Asn Gly Tyr Thr Asp Gly Tyr Ile Lys Tyr Val Thr
275 280 285
Ser Phe Ala Asn Lys Gly Phe Ala Asn Lys Gly Phe Asp Met Ala Asn
290 295 300
Gln Val Val Arg Gly Met Pro Asp Leu Val Arg Lys Arg Asp Glu Val
305 310 315 320
Ser Asn Glu Ile Asp Arg Asn Leu Arg Thr Gln Val Met Asn Leu Ile
325 330 335
Ile Gln Gly Thr Ser Leu Thr Leu Glu Glu Thr Leu Asn Lys Ser Ile
340 345 350
Asn Asn Ile Ala Phe Lys Gln Ala Ala Gly Ile Lys Ser Glu Trp Thr
355 360 365
Leu Asp Gln Asn Gly Arg Pro Phe Tyr Tyr Asp Leu Lys Gly Gln Met
370 375 380
Gln Thr Glu Trp Lys Glu Ile Ser Thr Ser Phe Ala Thr Lys Tyr Glu
385 390 395 400
Gly Thr Tyr Pro Leu Thr His Glu Ser Leu Lys Lys Leu Leu Pro Tyr
405 410 415
Ser Trp Tyr Tyr Phe Ser Pro Lys Lys Thr Asp Thr Phe Glu Lys Gly
420 425 430
Glu Met Tyr Ser Ser Thr Thr Gln Thr Ile Lys Asp Lys Glu Tyr Arg
435 440 445
Phe Asn Gln Glu Gly Lys Cys Leu Asn Pro Asp Gly Gln Pro Thr Asn
450 455 460
Asp Trp Val Gln Phe Asp Asn Arg Trp Tyr Tyr Phe Ser Pro Glu Asp
465 470 475 480
Ser Thr Lys Asn Thr Asp Gly Val Thr Phe Lys Lys Gly Ala Met Val
485 490 495
Thr Gly Asn Val Tyr Ile Asn Asn Lys Leu Tyr Gln Phe Asn Tyr Arg
500 505 510
Gly Glu Cys Lys Asn Pro Asn Ser Thr Asp Ser Val Ile Ser Asp Gly
515 520 525
Thr Tyr Lys Ile Val Tyr Pro Lys Ala Asn Lys Val Val Asp Phe Gly
530 535 540
Tyr Asp Gly Asn Glu His Pro Val Ile Trp Asp Tyr His Asn Gly Lys
545 550 555 560
Asn Gln Gln Trp Glu Phe Lys Tyr Asp Ala Ala Lys Asn Ala Tyr Gln
565 570 575
Ile Ile Asn Lys Asp Asp Gly Arg Val Leu Ala Tyr Asn Thr Ser Gly
580 585 590
Ala Ser Asp Thr Ala Leu Val Thr His Asn Asp His Lys Arg Glu His
595 600 605
Tyr Trp Thr Leu Glu Asp Ala Gly Asp Gly Asn Val Leu Leu Val Asn
610 615 620
Tyr Ala Asn Lys Asn Lys Val Leu Asp Val Tyr Gly Glu Lys Thr Ala
625 630 635 640
Glu Gly Ala Arg Leu Tyr Val Gln Asp Lys Leu Lys Asn Ser Asn Ala
645 650 655
Gln Lys Phe Lys Leu Val Lys Val Gly
660 665
<210> 88
<211> 516
<212> БЕЛОК
<213> Bacillus sp.
<400> 88
Met Lys Tyr Lys Asp Arg Lys His Ala Lys Arg Lys Tyr Thr Gln Ala
1 5 10 15
Leu Leu Ala Thr Val Ala Thr Met Ser Leu Gly Leu Ser Val Val Gly
20 25 30
Gly Thr Ala Pro Ala Phe Ala Glu Val Gln Thr Gln Thr Val Gln Glu
35 40 45
Leu Pro Lys Ser Phe Leu Asn Asp Leu Ser Gly Glu Phe Asn Ala Pro
50 55 60
Pro Ser Ile Asp Ser Ser Asp Val Lys Lys Leu Met Ile Asp Ala Ser
65 70 75 80
Gln Asp Ser Arg Lys Ile Asn Asp Leu Leu Arg Gln Ile Ser Met Gly
85 90 95
Val Ala Gly Lys Ile Pro Ala Val Gly Ser Val Val Ser Ala Met Val
100 105 110
Gly Tyr Leu Tyr Pro Glu Gln Asn Gly Thr Asp Ser Lys Leu Asn Ala
115 120 125
Leu Glu Ala Lys Leu Thr Ala Lys Ile Glu Lys Glu Val Asn Ala Gln
130 135 140
Arg Val Glu Asp Ile Lys Ser Arg Ile Lys Asn Leu Thr Asn Ala Ala
145 150 155 160
Asn Glu Leu Gln Val Ala Leu His Lys Thr Lys Ser Gly Ser Tyr Tyr
165 170 175
Asp Gly Ser Val Gly Asp Lys His Phe Thr Leu Arg Met Lys Ala Asp
180 185 190
Ala Val Asp Thr Ile Phe Lys Asp Leu Ile Ser Phe Leu Thr Gln Asp
195 200 205
Gly His Glu Ile Gly Asp Leu Pro Val Tyr Thr Lys Val Ala Ala Ala
210 215 220
His Ile Ile Phe Leu Asn Tyr Met Lys Thr Arg Gly Thr Asp Ser Gln
225 230 235 240
Leu Tyr Arg Tyr Asp Thr Pro Ser Thr Val Asp Ala His Phe Gln Pro
245 250 255
Thr Asn Lys Val Asn Thr Tyr Ala Lys His Ile Glu Glu Thr Phe Lys
260 265 270
Lys Gly Asp Asp Lys Ile Val Gln Leu Ile Asn Glu Leu Glu Asp Lys
275 280 285
Lys Glu Lys Leu Arg Asn Val Gly Ser Ser Thr Gly Gly Ser Leu Ala
290 295 300
Gly Gly Asn Leu Asn Ala Glu Arg Asp Arg Ser Arg Leu Asn Lys Glu
305 310 315 320
Ile Asp Gly Leu Lys Ala Leu Asn Leu Asn Arg Lys Arg Ser Leu Tyr
325 330 335
Arg Asp Leu Thr Val Ala Glu Gly Ser Phe Glu Ala Ala Ser Gly Lys
340 345 350
Lys Leu Thr Tyr Thr His Phe Asp Asp Val Leu Ser Gly Thr Tyr Lys
355 360 365
Ile Val Ser Lys Leu Asp Ser Ser Met Ile Leu Asp Asn Asp Tyr Gly
370 375 380
His Glu Arg Leu Ala Lys Leu Asn Lys Asp Tyr Thr Asp Lys Lys Glu
385 390 395 400
Glu Trp Val Asn Gly Glu Lys Trp Thr Leu Gln Tyr Asn Ala Asp Lys
405 410 415
Lys Ala Tyr Lys Ile Ile Asn Gln Lys Ala Pro Tyr Asp Glu Leu Ala
420 425 430
Ile Asn Gly Asn Ser Gly Asp Asp Thr Val Phe Ala Thr Thr Asp Arg
435 440 445
Lys Leu Asp Ser Glu Pro Thr Ala Asp Leu Arg Tyr Trp Lys Val Glu
450 455 460
Asp Ala Gly Asn Gly Tyr Phe Phe Ile Lys Asn Leu Lys Thr Gly Arg
465 470 475 480
Val Leu Asp Val Thr Asp Gly Lys Thr Asp Val Gly Thr Val Ile Lys
485 490 495
Thr His Ala Gln Asn Ala Ser Pro Val Ala Ala Gln Met Phe Lys Ile
500 505 510
Glu Thr Val Gln
515
<210> 89
<211> 569
<212> БЕЛОК
<213> Bacillus sp.
<400> 89
Leu Met Val Met Leu Gln Cys Pro Cys Thr Met Lys Pro Ile Tyr Ser
1 5 10 15
Ile Asn Asn Thr Thr Arg Lys Asp Asp Leu Met Lys Tyr Lys Asp Arg
20 25 30
Lys Asp Ala Gln Arg Lys Tyr Lys Gln Ala Ile Leu Thr Thr Val Ala
35 40 45
Thr Met Thr Leu Gly Val Ser Thr Leu Gly Pro Thr Ala Ser Val Phe
50 55 60
Ala Ala Glu Gly Asp Asn Ile Ser Val Ile Asn His Val Glu Thr Gln
65 70 75 80
Thr Glu Asn Ser Lys Leu Leu Glu Ser Lys Trp Met Asn Lys Phe Asn
85 90 95
Ala Glu Met Thr Asn Leu Lys Ser Val Gly Lys Phe Thr Gly Thr Thr
100 105 110
Met Ser Glu Ile Tyr Lys Phe Ala Asn Lys Gly Pro Glu Gln Leu Asn
115 120 125
Asp Thr Leu Arg Ala Ile Thr Ile Ala Ser Ala Asp Leu Ile Pro Tyr
130 135 140
Gly Gly Met Leu Val Ala Pro Leu Ile Ser Ala Ile Trp Pro Glu Thr
145 150 155 160
Asn Gly Ile Asn Ser Gln Leu Asn Ala Leu Arg Glu Asp Leu Thr Lys
165 170 175
Leu Val Thr Asn Glu Ile Asp Asn Glu Gln Leu Asn Phe Leu Asn Ser
180 185 190
Glu Phe Lys Thr Leu Ile Asp Leu Gln Thr Asp Leu Asp His Ala Val
195 200 205
Asn Ala Asp Asn Ser Gln Asn Thr Asp Glu Asn Thr Lys Asn Gln Arg
210 215 220
Gly Ala Trp Ala Asn Gly Ile Glu Gln Ser Phe Arg Arg Leu Leu Asp
225 230 235 240
His Thr Ser Ser Asp Lys His Lys Ile Thr Asn Leu Pro Ile Tyr Thr
245 250 255
Lys Val Ala Leu Ala His Leu Leu Phe Leu Asp Ser Leu Asn Asn Lys
260 265 270
Ala Val Ser Phe Lys Met Asn Ile Thr Glu Gln Ser Leu Lys Glu Leu
275 280 285
Tyr Thr Lys Asp Gly Val Lys Thr Leu Ala Asn Asp Tyr Val Leu His
290 295 300
Ile Met Lys Thr Phe Tyr Asp Ala Pro Gln Ile Lys Lys Ile Lys Gln
305 310 315 320
Val Pro Glu Phe His Phe Asn Pro Asp Asp Ala Glu Asn Gln Lys Lys
325 330 335
Asp Leu Lys Asp Arg Leu Ser Lys Val Glu Ala Glu Asn Tyr Gln Trp
340 345 350
Gln Met Asn Ile Ala Ser Ala Gly Gly Phe Gly Ser Ala Glu Asn Lys
355 360 365
Glu Tyr Lys Phe Lys Arg Ala Ile Leu Glu Asp Ala Ile Ser Ser Tyr
370 375 380
Glu Phe Val Glu Lys Leu Tyr Asn Thr Thr Val Gly Asp Pro Met Ile
385 390 395 400
Lys Glu Leu Val Lys Asp Ser Asp Asp Pro Met Ile Lys Glu Leu Ala
405 410 415
Lys Asp Ser Ser Glu Val Phe Val Ala Asp Gly Pro Tyr Lys Ile Val
420 425 430
Tyr Ser Lys Ala Asn Lys Val Val Asn Phe Gly Ser Asp Gly Ile Glu
435 440 445
His Pro Val Ile Gly Asp Phe His Tyr Gly Lys Asn Arg Glu Arg Gln
450 455 460
Trp Trp Gly Phe Lys Tyr Asp Ala Asp Lys Lys Ala Tyr Gln Ile Ile
465 470 475 480
Asn Arg Ala Asp Gly Arg Val Leu Ala Tyr Asn Thr Thr Pro Asn Ala
485 490 495
Asp Asp Thr Ala Leu Val Thr Glu Asn Gln His Lys Pro Glu His Tyr
500 505 510
Trp Ile Leu Glu Asp Ala Gly Ala Gly Asn Val Leu Leu Val Asn Tyr
515 520 525
Glu Asn Lys Asn Lys Val Leu Asp Val Tyr Asp Glu Lys Thr Asp Asp
530 535 540
Gly Ser Arg Val Tyr Val Arg Asp Lys Leu Asn Asn Ser Asn Ala Gln
545 550 555 560
Lys Phe Lys Leu Glu Phe Leu Asn Ile
565
<210> 90
<211> 394
<212> БЕЛОК
<213> Bacillus sp.
<400> 90
Met Lys Arg Gln Lys Ile Lys Lys Phe Pro Leu Ser Lys Lys Val Met
1 5 10 15
Thr Gly Leu Phe Ala Gly Ala Met Ala Leu Ser Ile Trp Ala Pro Ala
20 25 30
Ser Gln Ala Ala Thr Pro Glu Lys Asn Gly Tyr Tyr Ser Ile His Leu
35 40 45
Ala Ser Asn Thr Trp Lys Lys Trp Asp Val Thr Arg Gly Ser Leu Asp
50 55 60
Asn Glu Val Gly Ile Leu Leu Tyr Asp Val Ser Gly Gln Ile Pro Asp
65 70 75 80
Asn Gln Gln Phe Ala Phe Phe Pro Leu Asp Asn Gly Lys Tyr Ala Ile
85 90 95
Val Asn Lys Asn Ser Gly Lys Pro Val Thr Ile Gly Asp Trp Val Gln
100 105 110
Pro Met Ala Met Leu Thr Gly Tyr Asn His Asp Thr Leu Lys Gln Ser
115 120 125
Asn Trp Thr Gly Asn Ala Asn Glu Gln Trp Tyr Leu Arg Asp Gln Gly
130 135 140
Ser Asn Asn Tyr Glu Ile Val Asn Gln Gly Asn Gly Lys Val Ala Ser
145 150 155 160
Tyr Ala Trp Thr Gly Asp Leu Ala Arg Asn Tyr Glu Tyr Val Asp Leu
165 170 175
Asp Asn Ser Asn Pro Ser Asp Lys Asp Arg Val Phe Thr Ile Pro Ala
180 185 190
Ala Arg Ser Thr Phe Thr Leu Pro Thr Leu Pro Ala Thr Gly Thr Arg
195 200 205
Ser Glu Ala Pro Asn Tyr Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln
210 215 220
Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro Cys Ile Met
225 230 235 240
Val Lys Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro
245 250 255
Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Lys
260 265 270
Val Ile Gln Pro Gly Leu Ser Glu Ser Tyr Ser Tyr Lys Thr Gly Val
275 280 285
Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Phe
290 295 300
Gly Ala Asp Phe Gly Ile Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys
305 310 315 320
Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile Ser Thr Thr Asn Ser
325 330 335
Glu Ala Thr Glu Glu Thr Arg Ser Tyr Thr Ala Thr Gly Glu Pro Gly
340 345 350
Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys Tyr Thr Leu
355 360 365
Lys Arg Ala Asp Gly Ser Val Val Ser Asp Pro Trp Val Val Lys Asn
370 375 380
Asp Lys Ile Thr Val Thr Arg Lys Thr Ser
385 390
<210> 91
<211> 563
<212> БЕЛОК
<213> Bacillus sp.
<400> 91
Met Lys Tyr Lys Asp Arg Lys Asn Ala Lys Arg Lys Tyr Lys Gln Ala
1 5 10 15
Leu Leu Ala Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Ser Thr Ala Ser Val Phe Ala Ala Glu Asn Thr Gly Gln Thr Thr Thr
35 40 45
Gln Val Lys Glu Gly Phe Glu Arg Val Glu Pro Leu Ile Gln Pro Lys
50 55 60
Ile Gln Tyr Ala Asn Leu Asp Pro Lys Lys Ile Gly Glu Ala Val Thr
65 70 75 80
Gly Ala Phe Asp Asn Phe Thr Ser Phe Ile Leu Ser Lys Gln Lys Val
85 90 95
Ala Lys Leu Asp Glu Asn Gly Val Pro Val Arg Asp Glu Leu Gly Lys
100 105 110
Val Val Gln Val Ser Leu Asp Glu Lys Glu Ile Lys Lys Ile Lys Asp
115 120 125
Ser Leu Asn Lys Met Ser Lys Pro Gly Ala Ala Val Leu Lys Lys Ala
130 135 140
Ala Glu Gly Gly Asp Gly Gln Glu Ile Leu Val Ala Tyr Val Glu Ser
145 150 155 160
Leu Leu Ser Met Ala Pro Tyr Gly Ala Phe Ile Ser Pro Leu Leu Ser
165 170 175
Met Leu Leu Pro Glu Lys Lys Gly Glu Asn Ile Met Asp Gln Val Asn
180 185 190
Lys Ala Ile Asp Lys Lys Leu Lys Ser Gly Ile Glu Asn Tyr Asp Ile
195 200 205
Ala Gly Leu Asn Ala Ala Phe Glu Asn Val Glu Asp Ser Val Gln Thr
210 215 220
Ala Glu Asn Tyr Leu Ala Lys Asp Leu Lys Gly Asp Glu Arg Arg His
225 230 235 240
Leu Thr Asp Ser Met Lys Ala Lys Asn Lys Ala Leu Lys Asn Phe Leu
245 250 255
Thr Val Ala Ser Lys Lys Gly Tyr Glu Val Ala Glu Leu Pro Ile Tyr
260 265 270
Gly Val Ala Ala Ile Ala His Leu Gly Phe Leu Lys Ser Met Ser Tyr
275 280 285
Glu Ser Ala Arg Lys Ile Ala Glu Ile Pro Asp Ser Val Tyr Asp Ala
290 295 300
Glu Phe Thr Lys Phe Lys Asp Ala Ala Lys Thr Tyr Gly Asp His Ile
305 310 315 320
Asn Asp Thr Tyr Lys Met Lys Leu Val Glu Phe Glu Lys Asn Met Lys
325 330 335
Glu Ala Ile Glu Asp Gly Thr Asn Val Ser Ser Lys Glu Val Thr Ile
340 345 350
Asp Asn Tyr Lys Asp Val Leu Asn Lys Ala Tyr Asp Glu Ala Lys Asn
355 360 365
His Arg Thr Thr Thr Ser His Gly Ser Tyr Ser Ser Pro Glu Glu Lys
370 375 380
Arg Met Asn Ala Ile Thr Thr Ala Ile Gln Glu Cys Asp Ala Leu Ile
385 390 395 400
Asn Glu Leu Asn Lys Tyr Lys Asn Leu Thr Val Asn Asn Val Ala Leu
405 410 415
Asn Lys Leu Ile Tyr Asn Gln Asp Thr Asn Pro Asn Val Ala Val Ser
420 425 430
Lys Trp Ser Gln Leu Asp Asp Gly Lys Trp Tyr His Phe Asn Ser Val
435 440 445
Gly Gln Lys Asp Thr Gly Trp Phe Gln Asp Asn Asp Lys Ser Trp Tyr
450 455 460
Tyr Leu Gly Ser Asp Gly Ala Met Val Thr Gly Trp Tyr Lys Tyr Gly
465 470 475 480
Ser Ser His Phe Tyr Leu Asn Asp Thr Leu Lys Thr Asn Tyr Lys Gly
485 490 495
Glu Thr Val Lys Lys Gly Gln Met Ile Thr Gly Trp Tyr Tyr Asn Thr
500 505 510
Asp Asn Lys Tyr Tyr Tyr Leu Lys Pro Asp Asn Gly Ala Met Val Gln
515 520 525
Asn Asp Thr Ile Lys Ile Asp Gly Ala Asp Ala Arg Phe Asp Glu Asn
530 535 540
Gly Val Trp Gly Gly Gly Lys Val Glu Lys Ser Ser Asn Asp Lys Ser
545 550 555 560
Thr Arg Glu
<210> 92
<211> 393
<212> БЕЛОК
<213> Bacillus sp.
<400> 92
Val Ile Ser Asn Lys Arg Arg Asn Leu His Met Lys Lys Ala Cys Leu
1 5 10 15
Ile Gly Leu Val Ala Thr Thr Ile Ser Thr Ser Cys Leu Ser Thr Val
20 25 30
Thr Thr Leu Ala Ala Pro Leu Ser Glu Ser Gln Gln Val Gln Gly Val
35 40 45
Ala Ala Val Asn Thr Leu Ser Asn Ser Ile Lys Met Leu Gly Ile Gln
50 55 60
Ala Pro Leu Ile Gln Ala Tyr Gly Leu Val Ile Leu Gln Gln Pro Asp
65 70 75 80
Ala Arg Val Glu Ala Met Ser Ser Leu Thr Asn His Gln Ala Phe Ala
85 90 95
Lys Thr Asn Val Arg Glu Trp Leu Asp Glu Tyr Asn Pro Gln Ile Leu
100 105 110
Ala Met Asn Gln Glu Met Ile Arg Cys Asn Asn Arg Phe Asn Gly Tyr
115 120 125
Tyr Asn Lys Leu Leu Asp Leu Leu Gly Ser Leu Asn Asn Asn Glu Gln
130 135 140
Ala Lys Lys Asp Phe Ile Ile Ala Val Asp Gln Met Gln Gln Gln Ala
145 150 155 160
Gln Ala Ala Phe Glu His Leu Glu Gln Thr Ser Ser Ser Leu Asn Arg
165 170 175
Phe Lys Val Gln Leu Val Thr Asp Ser Lys Ser Leu Ser Glu Lys Ser
180 185 190
Asn Ile Ala Ile Gln Ser Leu Gln Gly Thr Asn Gly Asn Ile Glu Gln
195 200 205
Leu Arg Ala Glu Val Lys Arg Leu Gln Glu Glu Ile Gln Asn Asn Leu
210 215 220
Ile Thr Ile Leu Asn Arg Pro Asp Glu Ile Ile Lys Gly Ser Ile Asn
225 230 235 240
Ile Gly Lys Glu Leu Phe Lys Ile Thr Ser Ala Thr Ala Glu Ser Lys
245 250 255
Thr Ile Asn Phe Thr Ser Ile Cys Ser Leu Ser Glu Glu Phe Met Lys
260 265 270
Ala Thr Asp Ser Lys Thr Gln Gln Ala Thr Leu Asn Leu Gln Gln Lys
275 280 285
Gln Lys Glu Leu Leu Thr Val Ile Gln Lys Leu Ser Gln Thr Gln Val
290 295 300
Gln Ala Thr Glu Ile Thr Phe Ile Glu Asp Gln Val Asn Ser Phe Ala
305 310 315 320
Glu Leu Ile Ser Arg Gln Val Ala Thr Phe Asp Thr Val Ile Gly Asp
325 330 335
Trp Lys Leu Phe Glu Glu Gly Leu Thr Gln Leu Lys Ala Asn Ser His
340 345 350
Val Gln Gly Asn Leu Asn Thr Thr Asp Leu Gln Lys Gln Leu Ile Gln
355 360 365
Leu Lys Glu Trp Asn Asp Met Leu Asn Lys Gln Thr Lys Gln Phe Glu
370 375 380
Asp Ser Val Met Gln Val Thr Val Gln
385 390
<210> 93
<211> 432
<212> БЕЛОК
<213> Bacillus sp.
<400> 93
Leu Val Leu Ser Phe Tyr Ile Ala Lys Val Ile Leu Phe Arg Asn Lys
1 5 10 15
Ser Ile Ser Asn Val Gln Tyr Asn Phe Ile Phe Cys Asn Leu Val Gly
20 25 30
Ile Leu Pro Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys
35 40 45
Lys Val Met Ala Gly Leu Gly Val Gly Ala Met Ser Leu Ser Ile Trp
50 55 60
Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala Asn Asn Glu Tyr His Thr
65 70 75 80
Ile His Leu Ala Ala Asn Thr Asn Ile Ala Trp Asp Val Tyr Lys Gly
85 90 95
Trp Gln Ser Asp Asp Ala Gly Ile Val Leu Trp Asp Gly Ala Arg Gly
100 105 110
Asp Asn Glu Gln Phe Val Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala
115 120 125
Ile Val Asn Lys Asn Ser Gly Lys Pro Val Gly Ile Gly Ser Ala Ile
130 135 140
Ile Gly Ser Asp Gly Val Arg Asn Phe Pro Ser Phe Asn Asn Glu Gly
145 150 155 160
Leu Arg Gln Thr Ser Trp Thr Gly Ala Pro Asn Glu Gln Trp Tyr Leu
165 170 175
Arg Asp Lys Gly Asn Asn Asn Phe Glu Ile Val Asn Gln Gly Ser Gly
180 185 190
Lys Val Ala Ser Trp Ala Gly Val Glu Met Tyr Gly Tyr Lys Asp Tyr
195 200 205
Val Asp Leu Asp Glu Ser Asn Pro Ser Asp Asn Asp Arg Leu Phe Asn
210 215 220
Ile Pro Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu Pro Thr Val
225 230 235 240
Gly Thr Arg Pro Asn Ala Pro Asp Tyr Asn Thr Ser Gly Ala Ile Asp
245 250 255
Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu
260 265 270
Ile Pro Ser Ile Met Val Lys Asp Asn Gly Ala Ser Asp Tyr Thr Lys
275 280 285
Ile His Asn Ser Pro Tyr Tyr Val Leu Val Lys Glu Glu Tyr Trp Glu
290 295 300
Lys Val Arg Ser Glu Ile Ile Pro Ala Gly Ala Thr Ser Lys Tyr Thr
305 310 315 320
Val Lys Ser Gly Val Ser Glu Ile Asp Gln Lys Lys Met Ser Asp Thr
325 330 335
Leu Ala Met Ser Phe Gly Ser Asp Leu Gly Leu Lys Phe Gly Glu Ser
340 345 350
Ser Leu Ser Ile Lys Ser Ser Val Ser Arg Thr Ile Gln Thr Glu Val
355 360 365
Thr Lys Thr Ser Thr Asn Ala Thr Glu Glu Thr Glu Glu Lys Ser Ile
370 375 380
Pro Ser Ile Ser Gly Lys Asp Thr Gly Phe Thr Ala Tyr Gln Leu Ala
385 390 395 400
Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly Ser Val Val Thr Asp Pro
405 410 415
Ser Ile Val Arg Asp Ser Lys Gln Thr Ile Ala Arg Thr Ile Ala Lys
420 425 430
<210> 94
<211> 383
<212> БЕЛОК
<213> Bacillus sp.
<400> 94
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ala Ser Gln Ala Ala Pro Ala Lys
20 25 30
Asn Ala Tyr His Thr Ile Gln Leu Ala Ala Asn Ala Trp Leu Lys Trp
35 40 45
Asp Val Tyr Gly Asn Gly Thr Tyr Asp Asp Ala Gly Ile Leu Leu Tyr
50 55 60
Asn Gly Ser Phe Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu Asp
65 70 75 80
Gly Gly Ser Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Ala Val Thr
85 90 95
Thr Gly Ser Gly Trp Ile Ala Gly Asn Thr Met Ser Tyr Asn Pro Glu
100 105 110
Gly Leu Lys Gln Ser Lys Trp Thr Gly Ala Ala Ala Asn Lys Trp Tyr
115 120 125
Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly Tyr
130 135 140
Gly Lys Val Ala Ser Tyr Ala Arg Ile Gly Ile Ala Gly Pro Gln Tyr
145 150 155 160
Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn Asp Arg Leu Phe His
165 170 175
Ile Pro Ala Ala Ala Ser Ser Thr Phe Ser Leu Pro Thr Leu Pro Asn
180 185 190
Val Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Ala Gly Ile Asp Gln
195 200 205
Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Val
210 215 220
Pro Cys Ile Met Val Asn Asp Asn Gln Ala Ser Asp Tyr Thr Lys Ile
225 230 235 240
His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys
245 250 255
Thr Phe Ser Ala Val Val Pro Ala Gly Leu Asn Leu Lys Tyr Ser Phe
260 265 270
Lys Thr Gly Met Thr Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu
275 280 285
Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Ala Ser
290 295 300
Ala Ala Ile Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile Ser
305 310 315 320
Thr Ser Asn Thr Glu Val Ser Glu Glu Thr Ile Met Arg Glu Ala Ser
325 330 335
Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr Glu Tyr Gln Leu Ala Thr
340 345 350
Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val Ser Asp Pro Trp
355 360 365
Val Val Lys Asn Asn Lys Ile Thr Ile Ala Arg Lys Asn Ala Gln
370 375 380
<210> 95
<211> 420
<212> БЕЛОК
<213> Bacillus sp.
<400> 95
Met Ile Leu Phe Lys Glu Lys Ser Ile Ser Asn Val Gln Tyr Asn Phe
1 5 10 15
Ile Phe Cys Asn Leu Val Glu Ile Leu Leu Ala Lys Gln Leu Gly Gly
20 25 30
Asn Met Lys Val Leu Ser Lys Lys Thr Leu Ala Gly Leu Met Ala Gly
35 40 45
Ala Leu Ser Leu Ser Ile Trp Ala Pro Ala Ser Glu Ala Ala Thr Pro
50 55 60
Lys Lys Asn Gln Tyr Tyr Ser Ile Lys Leu Lys Ala Asn Gln Asn Leu
65 70 75 80
Ala Trp Asp Val Tyr Gly Gly Arg Ile Asp Pro Leu Thr Arg Val Thr
85 90 95
Leu Tyr Asp Ala His Asn Asn Glu Asn Gln Lys Phe Val Phe Phe Pro
100 105 110
Leu Asp Gly Gly Lys Tyr Ala Ile Val Asn Lys Asn Ser Gly Ile Pro
115 120 125
Val Ser Gly Asp Leu Ser Lys His Trp His Trp Phe Ser Ser Gln Thr
130 135 140
Leu Gly Gln Ile Gly Trp Thr Gly Ala Pro Asp Gln Gln Trp Tyr Leu
145 150 155 160
Arg Asp Gln Gly Ser Asn Tyr Tyr Glu Ile Val Asn Gln Glu Ser Gly
165 170 175
Glu Val Ala Ser Tyr Gly Thr His Asp Glu Asn Asn Gly Ser Ile Ala
180 185 190
Gly Ala Phe Ser Tyr Arg Leu Gly Leu Gly Tyr Ser Asn Pro Ser Asn
195 200 205
Ser Asn Gly Val Phe Lys Leu Thr Ala Thr Asn Asn Ile Ser Leu Pro
210 215 220
Lys Leu Pro Ser Val Gly Thr Arg Pro Ser Ala Pro Lys Tyr Thr Gly
225 230 235 240
Asp Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Phe Val Val Val Gly
245 250 255
Ala Ser Leu Val Pro Ser Ile Met Val Asn Asp Asn Gln Val Ser Asp
260 265 270
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Val Leu Val Lys Glu Glu
275 280 285
Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg
290 295 300
Gln Tyr Ser Phe Lys Thr Gly Met Thr Ser Thr Asp Gln Gln Lys Met
305 310 315 320
Thr Glu Thr Leu Ser Met Ser Ile Gly Ala Asp Leu Gly Leu Lys Phe
325 330 335
Gly Glu Ser Thr Ala Ser Ile Lys Ser Asn Ile Thr Lys Thr Leu Gln
340 345 350
Thr Glu Ile Ser Thr Thr Asn Thr Gln Ala Gln Glu Glu Thr Ile Thr
355 360 365
Ala Thr Ala Glu Ser Lys Ser Gly Lys Thr Thr Ala Phe Thr Glu Tyr
370 375 380
Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val
385 390 395 400
Ser Asn Pro Trp Val Val Lys Asn Asn Lys Val Thr Ile Ala Arg Asp
405 410 415
Asn Val Gln Glu
420
<210> 96
<211> 415
<212> БЕЛОК
<213> Bacillus sp.
<400> 96
Leu Ser Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Leu Ser Lys Lys
1 5 10 15
Val Met Ala Gly Leu Leu Val Gly Ala Thr Ser Leu Ser Ile Trp Ala
20 25 30
Pro Thr Ser Glu Ala Ala Ala Pro Glu Lys Asn Arg Tyr Tyr Ser Leu
35 40 45
His Leu Thr Asn Asn Ile Glu Lys Val Trp Asp Val Tyr Asn Gly Trp
50 55 60
Thr Ser Asn Asp Ala Gly Ile Leu Leu Tyr Lys Ala Asn Gly Gln Asp
65 70 75 80
Asn Gln Gln Phe Val Phe Phe Gln Leu Asp Gly Gly Thr Tyr Ala Ile
85 90 95
Val Asn Lys Asn Ser Gly Lys Pro Val Gly Leu Gly Gly Gly Ile Asn
100 105 110
Tyr Thr Asp Gly Val Arg Asn Met Ser Asn Ile Ala Gly Asn Pro Ala
115 120 125
Asp Thr Leu Gly Gln Asn Asp Trp Thr Gly Ala Asp Thr Asp Lys Trp
130 135 140
Tyr Leu Arg Asp Lys Gly Asn Gly Asn Tyr Glu Ile Val Asn Gln Gly
145 150 155 160
Asn Gly Arg Val Ala Ser Tyr Ala Ala Thr Asn Ile Ala Pro Tyr Leu
165 170 175
His Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Gly Asp Arg Leu
180 185 190
Phe Lys Val Ser Lys Ser Pro Phe Ala Phe Gly Ile Asp Ile Pro Gly
195 200 205
Phe Asn Phe Asp Tyr Gly Thr Phe Ser Leu Pro Gln Leu Pro Ala Thr
210 215 220
Gly Asn Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Ile Asp Gln Gln
225 230 235 240
Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro
245 250 255
Cys Phe Met Val Lys Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile His
260 265 270
Asn Ser Pro Tyr Tyr Thr Leu Ile Lys Glu Glu Tyr Trp Asp Lys Thr
275 280 285
Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg Asn Tyr Thr Phe Lys
290 295 300
Thr Gly Met Thr Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser
305 310 315 320
Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Ala Ser Ala
325 330 335
Ser Leu Lys Thr Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile Thr Ser
340 345 350
Thr Asn Thr Glu Ala Ser Glu Glu Thr Ile Ser Ser Thr Val Thr Ser
355 360 365
Glu Pro Gly Lys Ser Gln Thr Gly Phe Thr Glu Tyr Gln Leu Ala Thr
370 375 380
Lys Tyr Thr Leu Lys Arg Ala Asp Gly Thr Ile Val Ser Asp Pro Trp
385 390 395 400
Val Val Lys Asn Asn Lys Ile Thr Ile Ala Arg Lys Asn Ala Gln
405 410 415
<210> 97
<211> 503
<212> БЕЛОК
<213> Bacillus sp.
<400> 97
Met Lys Tyr Lys Gln Val Leu Leu Ala Thr Val Ala Thr Met Thr Leu
1 5 10 15
Gly Val Ser Thr Leu Gly Gly Thr Ala Ser Val Phe Ala Ala Glu Gln
20 25 30
Pro Val Gln Glu Leu Pro Lys Leu Leu Asn Asp Leu Ser Gly Glu Phe
35 40 45
Asn Ser Pro Leu Ile Lys Ser Glu Glu Val Ser Lys Ile Met Ser Asp
50 55 60
Ala Ser Lys Asp Ala Arg Arg Leu Asn Asp Leu Leu Arg Gln Ile Ser
65 70 75 80
Met Gly Val Ala Gly Lys Ile Pro Ala Val Gly Ser Val Val Ser Ala
85 90 95
Met Val Gly Tyr Leu Tyr Pro Glu Gln Asp Gly Thr Asp Ser Lys Leu
100 105 110
Asn Ala Leu Glu Ala Lys Leu Thr Ala Lys Ile Glu Gln Ala Val Asn
115 120 125
Asp Gln His Val Lys Asp Ile Lys Ser His Ile Gln Asn Leu Thr Asn
130 135 140
Ala Ala Asn Glu Leu Gln Thr Ala Leu Asn Ser Val Asn Lys Gly Ala
145 150 155 160
Phe Tyr Asp Gly Gly Ser Ile Lys Asp Ile His Gly Thr Leu Arg Glu
165 170 175
Lys Ala Glu Thr Val Asp Lys Lys Phe Lys Glu Leu Met Pro Phe Leu
180 185 190
Met Gln Glu Gly His Glu Ile Gly Asp Leu Pro Val Tyr Thr Lys Val
195 200 205
Ala Ala Ala His Ile Leu Phe Leu Asn Tyr Met Lys Thr Arg Gly Thr
210 215 220
Asp Ser Lys Leu Tyr Arg Tyr Asp Ser Asp Lys Thr Val Asp Ala Gln
225 230 235 240
Phe Gln Pro Thr Asp Arg Val Asp Thr Tyr Ala Lys His Ile Glu Asp
245 250 255
Thr Phe Lys Lys Gly Asp Asp Lys Ile Val Gln Leu Ile Lys Glu Leu
260 265 270
Glu Asp Lys Lys Glu Ala Leu Arg Asn Val Gly Ser Ser Thr Gly Gly
275 280 285
Ser Leu Ala Gly Gly Asn Leu Gly Ala Glu Asn Lys Arg Ser Ile Leu
290 295 300
Asn Lys Glu Ile Asp Gly Leu Lys Ala Leu Asn Leu Asn Arg Lys Arg
305 310 315 320
Ser Leu Tyr Arg Asp Leu Thr Val Ala Glu Gly Ser Phe Glu Ala Ala
325 330 335
Ser Gly Lys Lys Leu Thr Tyr Thr His Phe Ala Asp Ile Leu Ser Gly
340 345 350
Thr Tyr Lys Ile Val Ser Lys Leu Asp Ser Ser Met Ile Leu Asp Asn
355 360 365
Asp Tyr Gly His Glu Arg Leu Ala Lys Leu Asn Lys Asp Tyr Thr Asp
370 375 380
Lys Lys Glu Glu Trp Val Asn Gly Glu Lys Trp Thr Phe Gln Tyr Asn
385 390 395 400
Ala Asp Lys Lys Ala Tyr Lys Ile Ile Asn Gln Lys Ala Pro Tyr Asp
405 410 415
Glu Leu Ala Ile Asn Gly Lys Phe Gly Asp Asp Thr Val Phe Ala Thr
420 425 430
Thr Asp Arg Lys Leu Asp Ser Asp Ser Thr Ala Asp Leu Arg Tyr Trp
435 440 445
Lys Val Glu Asp Ala Gly Asn Gly Tyr Phe Phe Ile Lys Asn Leu Lys
450 455 460
Thr Gly Arg Val Leu Asp Val Thr Asp Gly Lys Thr Gly Val Gly Thr
465 470 475 480
Val Ile Lys Thr His Ala Gln Asn Ala Ser Pro Val Ala Ala Gln Met
485 490 495
Phe Lys Ile Glu Thr Val Gln
500
<210> 98
<211> 590
<212> БЕЛОК
<213> Bacillus sp.
<400> 98
Met Lys Tyr Lys Asn Arg Lys Asp Ala Lys Arg Lys Tyr Arg Lys Val
1 5 10 15
Ala Leu Ala Thr Val Val Thr Met Thr Leu Gly Val Ser Ala Val Gly
20 25 30
Asn Thr Ala Ala Ile Phe Ala Asp Glu Thr Ser Thr Lys Thr Arg Gln
35 40 45
Thr Ala Pro Glu Ser Leu Gly Ala Asn Gly Ser Gly Ala Gly Ala Thr
50 55 60
Thr Ser Lys Leu Glu Glu Leu Leu Thr Met Asp Asn Leu Lys Lys Val
65 70 75 80
Gly Thr Leu Ser Gly Asp Val Leu Lys Val Ala Tyr Lys Asp Ala His
85 90 95
Glu Asn Asn Gly Asn Tyr Asn Asn Thr Phe Arg Thr Leu Ala Met Ser
100 105 110
Gly Thr Ala Leu Ile Pro Tyr Gly Gly Val Ile Ile Ser Pro Leu Ile
115 120 125
Gly Leu Leu Trp Pro Glu Asn Val Glu Ala Gln Lys Asn Gln Met Lys
130 135 140
Lys Leu Ile Asp Glu Leu Asn Asn Met Met Asp Gln Lys Ile Thr Asp
145 150 155 160
Asn Glu Ile Ala Thr Ile Glu Ser Glu Val Asn Ile Leu Lys Glu Met
165 170 175
Leu Gln Lys Phe Glu Lys Asn Ile Asn Met Asn Ile Ser Ala Gly Phe
180 185 190
Phe Tyr Ser Pro Thr Thr Lys Glu Gln Ala Leu Ala Gln Asp Ala Lys
195 200 205
Ala Ile Asn Asn Lys Phe Ser Asp Ile Ile Gly His Cys Lys Gln Lys
210 215 220
Leu Gln Val Ile Pro Ala Leu Pro Ile Phe Thr Ala Met Ala Thr Ala
225 230 235 240
His Leu Glu Phe Leu Tyr Phe Leu Glu Lys Asn Gly Thr Gly Pro Lys
245 250 255
Leu Gln Tyr Thr Thr Asp Ala Leu Asn Thr Leu Leu Gln Gly Glu Asp
260 265 270
Asn Glu Gly Phe Gly Gln Arg Ile Asn Ser Leu Ala Glu Thr Tyr Val
275 280 285
Asn His Val Gln Lys Thr Tyr Gln Leu Gly Lys Gln Gln Phe Asn Lys
290 295 300
Lys Met Asp Asp Thr Ala Lys Asn Thr Pro Gly Gly Asn Thr Asn Ser
305 310 315 320
Thr Ser Glu Gln Glu Thr Met Ser Lys Met Gln Lys Tyr Ile Phe Asp
325 330 335
Leu His Pro Val Ala Asn Asn Ala Asp Glu Lys Arg Lys Gln Met Gln
340 345 350
Val Ala Leu Asn Asn Tyr Val Lys Leu Arg Asn Asp Arg Asp Gln Tyr
355 360 365
Tyr Lys Asn Thr Ala Asn Asn Glu Ala Phe Gln Leu Leu Ala Ile Gly
370 375 380
Lys Trp Lys Arg Glu Asp Asn Asn Trp Tyr Phe Thr Asp Thr Lys Gly
385 390 395 400
Glu Lys Lys Thr Gly Trp Ile Gln Leu Gly Glu Lys His Tyr Tyr Leu
405 410 415
Asn Pro Lys Asn Gly Ile Met Val Lys Gly Phe Val Thr Ile Asp Gly
420 425 430
Lys Pro Tyr Gln Phe Asn Asp Ser Gly Glu Leu Ile Phe Ser Glu Gly
435 440 445
Thr Tyr Lys Ile Val Tyr Ser Lys Ala Asn Lys Val Val Asp Phe Gly
450 455 460
Ala Tyr Gly Glu Gln Pro Val Ile Trp Asp Tyr His Pro Asp Trp Asn
465 470 475 480
Thr Pro Asn Thr Asn Gln Gln Trp Glu Phe Lys Tyr Asp Ala Ser Asn
485 490 495
Asn Ala Tyr Gln Ile Ile Asn Lys Gln Asn Lys Lys Val Leu Ala Tyr
500 505 510
Asn Thr Thr Ser Gly Thr Asp Tyr Thr Ala Leu Val Thr Glu Asn Gln
515 520 525
His Lys Ala Glu His Tyr Trp Thr Leu Glu Asp Ala Gly Asp Gly Asn
530 535 540
Ile Leu Leu Val Asn Phe Ala Asn Lys Asn Lys Val Leu Asp Val Tyr
545 550 555 560
Gly Glu Glu Thr Ala Asn Gly Ser Lys Val Tyr Val Arg Asp Lys Leu
565 570 575
Asn Asn Ser Met Ala Gln Lys Phe Lys Leu Ile Lys Leu Asp
580 585 590
<210> 99
<211> 374
<212> БЕЛОК
<213> Pseudomonas sp.
<400> 99
Met Lys Val Asn Met Met Ser Glu Asp Arg Lys Pro Asn Ile Gly Pro
1 5 10 15
Lys Asp Phe Ala Thr Val Glu Gly Ser Pro Phe Leu Leu Ser Gln Lys
20 25 30
Glu Trp Tyr Ala Ile Gln Arg Tyr Val Ala Ser Ala Leu Ala Leu Pro
35 40 45
Asp Asp Glu Lys Thr Met Arg Lys Asp Leu Ala Thr Thr Thr Ser Thr
50 55 60
Gly Glu Lys Thr Pro Tyr Met Gly Pro Phe Asp Glu Phe Lys Pro Leu
65 70 75 80
Leu Glu Gly Tyr Gln Glu Ile Ile Pro His Val Lys Val Trp Gln Asp
85 90 95
Asn Val Phe Pro Asn Thr Val Glu Leu Ala Lys Asp Ile Ala Asp Tyr
100 105 110
Ala Thr Phe Ala Gly Thr Tyr Tyr Gly Ala Leu Ile Pro His Ile Glu
115 120 125
Thr Leu Ser Leu Asp Pro Gly Asn Thr Glu Ala Lys Asp Lys Ile Ala
130 135 140
Asn Ile Cys Lys Lys Leu Ala Asn Glu Ala Lys Asn Asn Glu Asp Arg
145 150 155 160
Ala Val Ala Val Phe Asn Glu Ile Ser Leu Phe Ala Lys Gln Thr Ser
165 170 175
Asp Asp Glu Ile Leu Leu Lys Asn Leu Phe Gln Glu Tyr Glu Gly Lys
180 185 190
Tyr Gly Lys His Ser Ala Leu Leu Lys Ala Asn Arg Glu Glu Arg Asp
195 200 205
Ala Leu Ala Thr Lys Ile Asp Glu Met Asn Lys Gln Tyr Glu Tyr Asp
210 215 220
Cys Ile Val Ala Glu Thr Thr Pro Thr Tyr Ala Trp Ile Val Phe Trp
225 230 235 240
Val Ala Leu Ile Val Ala Ser Ile Tyr Gly Asp Arg Ala Ala Lys Leu
245 250 255
Lys His Glu Ile Asp Leu Lys Lys Asp Arg Val Glu Lys Leu Lys Ala
260 265 270
Glu Glu Glu Val Ala Val Ala Leu Met Gly Thr Leu Asn Val Ala Leu
275 280 285
Lys Gly Leu Ser Asp Ile Gln Glu Gln Ile Glu Ala Ala Leu Pro Cys
290 295 300
Ile Gln Lys Ile Lys Gly Val Trp Asn Ala Ile Gly Gly Asp Leu Asn
305 310 315 320
Lys Ile Gly Lys Ile Ile Lys Asp Asp Ile Leu Asn Glu Asp Asp Leu
325 330 335
Lys Asp Leu Gly Val Ala Asn Ala Ile Ala Met Trp Ala Gln Cys Lys
340 345 350
Leu Lys Ala Asp Ala Tyr Arg Ala Leu Ala Trp Ile Thr Pro Pro Thr
355 360 365
Lys Glu Glu Lys Ala Ala
370
<210> 100
<211> 393
<212> БЕЛОК
<213> Bacillus sp.
<400> 100
Met Lys Ser Met Ser Lys Lys Ile Met Thr Gly Leu Ala Ala Gly Ala
1 5 10 15
Met Gly Leu Ser Ile Trp Ala Pro Ser Ser Leu Ala Ala Thr Pro Glu
20 25 30
Lys Asn Glu Tyr His Thr Ile His Leu Ala Ala Asn Ser Asn Ile Val
35 40 45
Trp Asp Val Phe Lys Gly Trp Thr Thr Asp Asp Ala Gly Ile Ile Leu
50 55 60
Trp Asn Gly Asp Arg Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Asn Asn Ser Tyr Ala Tyr Tyr Trp Thr Asp Gly Val Arg Asn
100 105 110
Ala Gln Val Ile Asn Asp Gln Ala Leu Lys Gln Thr Asn Trp Thr Gly
115 120 125
Ala Pro Ala Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Phe
130 135 140
Glu Ile Val Asn Gln Gly Ser Gly Lys Val Ala Ser Trp Ala Gly Gln
145 150 155 160
Ser Ile Thr Gly Gly Tyr Leu Asp Tyr Val Asp Leu Asp Glu Ser Asn
165 170 175
Pro Ser Asp Asn Asn Lys Leu Phe Asn Ile Pro Ala Ala Arg Ser Thr
180 185 190
Phe Ser Leu Pro Thr Leu Pro Ala Val Gly Ser Arg Pro Asn Ala Pro
195 200 205
Glu Tyr Asn Leu Thr Gly Pro Ile Asp Gln Gln Leu Ala Gln Thr Ser
210 215 220
Asn Ser Ala Val Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Lys
225 230 235 240
Asp Ser Gly Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr
245 250 255
Val Leu Gln Lys Glu Glu Tyr Trp Glu Lys Thr Arg Ser Ala Thr Ile
260 265 270
Ser Pro Gly Gly Thr Asp Lys Tyr Phe Val Lys Thr Gly Val Ser Thr
275 280 285
Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Asn Phe Gly Val
290 295 300
Asp Leu Gly Leu Lys Phe Gly Asp Gln Ser Ala Ser Ile Lys Tyr Gly
305 310 315 320
Val Ser Lys Thr Leu Gln Thr Glu Val Ser Lys Thr Thr Thr Asn Ala
325 330 335
Thr Glu Glu Thr Glu Glu Lys Ser Ile Pro Ser Ile Asp Gly Lys Glu
340 345 350
Thr Gly Tyr Thr Val Tyr Gln Leu Val Thr Lys Tyr Thr Leu Lys Arg
355 360 365
Thr Asp Gly Ser Thr Val Ser Asp Pro Trp Thr Val Arg Asp Lys Asn
370 375 380
Gln Ile Val Val Arg Ser Ile Ala Lys
385 390
<210> 101
<211> 430
<212> БЕЛОК
<213> Bacillus sp.
<400> 101
Val Ile Leu Phe Arg Asn Lys Ser Ile Ser Asn Val Gln Tyr Asn Ile
1 5 10 15
Ile Phe Cys Asn Leu Val Gly Ile Leu Pro Thr Lys Leu Gly Gly Asn
20 25 30
Ile Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly
35 40 45
Ala Thr Ser Leu Ser Ile Trp Ala Pro Ser Ser Glu Ala Ala Ala Pro
50 55 60
Glu Lys Asn Arg Tyr Tyr Ser Ile His Leu Gln Ala Asn Lys Asn Leu
65 70 75 80
Ala Trp Asp Val Ser Gly Asn Ser Pro Asp Asn Asn Arg Ala Ile Leu
85 90 95
Leu Trp Asp Gly Ala Ser Gly Asp Asn Glu Gln Phe Val Phe Phe Pro
100 105 110
Leu Asp Gly Gln Asn Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Thr
115 120 125
Val Thr Phe Gly Gly Ser Thr Pro Ala Ile Tyr Gly Asn Asn Asp Gly
130 135 140
Leu Pro Asn Ser Glu Ala Phe Gln Gln Arg Ser Trp Thr Gly Ser Pro
145 150 155 160
Thr Glu Gln Trp Tyr Leu Arg Asp Gln Gly Ser Asn Asn Tyr Glu Ile
165 170 175
Val Asn Gln Gly Asn Gln Lys Val Ala Ser Tyr Ser Trp Ala Gly Ser
180 185 190
Leu Ala Gln His Val Glu Tyr Val Asp Leu Asp Glu Ser Lys Pro Ser
195 200 205
Asp Ser Asn Arg Val Phe Tyr Ile Ser Ser Gly Pro Val Asn Lys Gly
210 215 220
Val Gln Pro Ala Tyr Gly Thr Ile Ser Leu Pro Thr Leu Pro Ala Thr
225 230 235 240
Gly Thr Arg Pro Glu Ala Pro Lys Tyr Thr Asp Gly Gly Ala Ile Asp
245 250 255
Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu
260 265 270
Ile Pro Cys Ile Met Val Lys Asp Ser Gln Ala Ser Asp Tyr Thr Lys
275 280 285
Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp
290 295 300
Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg Asn Tyr Thr
305 310 315 320
Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln Lys Met Thr Asp Thr
325 330 335
Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Ala
340 345 350
Ser Ala Ser Ile Lys Ser Glu Val Ser Lys Thr Ile Gln Thr Glu Ile
355 360 365
Ser Thr Thr Asn Thr Glu Ala Thr Glu Glu Thr Ile Thr Ser Thr Val
370 375 380
Thr Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr Glu Tyr Gln Leu Ala
385 390 395 400
Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ala Val Ser Asp Pro
405 410 415
Trp Val Val Lys Asn Asn Lys Ile Thr Val Ala Arg Lys Ile
420 425 430
<210> 102
<211> 388
<212> БЕЛОК
<213> Mucilaginibacter sp.
<400> 102
Met Ser Asn Pro Thr Ile Asp Leu Gly Pro Arg Asp Leu Ala Lys Gly
1 5 10 15
Thr Thr Phe Val Leu Ser Thr Asn Glu Trp Leu Ser Ile Gln Val Phe
20 25 30
Val Thr Gly Ala Met Leu Leu Pro Thr Thr Thr Ala Leu Leu Gln Lys
35 40 45
Thr Leu Pro Ala Val Pro Ser Gly Gly Ile Glu Gln Phe Glu Pro Leu
50 55 60
Val Lys Gly Tyr Lys Asn Leu Tyr Asp Ile Cys Asp Thr Phe Asp Thr
65 70 75 80
Val Thr Lys Pro Asp Ser Val Lys Cys Ala Ser Asn Leu Ile Ala Tyr
85 90 95
Asn Val Lys Val Pro Ile Phe Tyr Arg Ala Ile Leu Ala Leu Leu Leu
100 105 110
Lys Ile Glu Ala Asp Pro Thr Asp Thr Val Ser Ile Asn Gln Leu Lys
115 120 125
Gln Thr Leu Gln Val Met Ile Asn Glu Ala Gln Ala Phe Ser Thr His
130 135 140
Ala Thr Asn Val Ala Ala Ala Met Gln Lys Phe Ser Leu Asp Thr Ser
145 150 155 160
Val Asn Glu Ala Ala Ile Gly Thr Leu Phe Lys Thr Tyr Ser Asp Lys
165 170 175
Ile Ser Gly Thr Ser Gly Thr Ile Lys Thr Met Gln Ala Gln Leu Ala
180 185 190
Gln Asp Lys Ala Asp Leu Ala Asp Ala Glu Asp Glu Tyr Lys His Asp
195 200 205
Ile Ile Val Ala Ala Thr Ser Ala Ser Tyr Ala Trp Ile Trp Pro Cys
210 215 220
Gly Ser Ile Ala Ala Gly Ile Val Ala Gly Ile Tyr Gly Asp Lys Ala
225 230 235 240
Thr Lys Ala Lys Ala Arg Ile Asp Ala Leu Gly Asp Leu Val Asn Ser
245 250 255
Leu Glu Ala Lys Ile Ala Ala Asp Ala Thr Leu Val Leu Asp Leu Thr
260 265 270
Arg Ile Asn Asn Asp Ile Thr Gly Ile Thr Lys Lys Ile Asp Asp Ala
275 280 285
Leu Pro Ala Ile Gln Lys Ile Gln Gly Met Trp Asn Ala Leu Lys Asp
290 295 300
Asp Leu Ser Ser Leu Leu Ala Thr Ile Asn Gly Glu Ile Lys Asp Met
305 310 315 320
Pro Ser Phe Ile Ala Gly Leu Gly Ile Glu Ala Ala Ile Val Leu Trp
325 330 335
Gln Gln Val Ala Asp Glu Ala Asn Ala Tyr Arg Ile Asn Ala Tyr Ile
340 345 350
Thr Ile Val Pro Asn Pro Thr Val Ala Phe Tyr Ala Ala Asp Asn Leu
355 360 365
Lys Lys Leu Leu Thr Arg Lys Asn Arg Val Ser Ile His Gln Lys Glu
370 375 380
Ser Ala Leu Ala
385
<210> 103
<211> 398
<212> БЕЛОК
<213> Bacillus sp.
<400> 103
Leu Gly Val Glu Met Asn Lys Glu Ala Gly Val Phe Met Lys Lys Ser
1 5 10 15
Ser Phe Val Thr Gly Cys Ile Ile Thr Ala Leu Met Ala Gly Gln Thr
20 25 30
Val Ser Ser Gln Val Phe Ala Ser Lys Lys Gln Gln Thr Asp Glu Gln
35 40 45
Ile Asn Thr Pro Ile Ala Thr Ser Thr Thr Ser Ser Ala Leu Gln Glu
50 55 60
Leu Gly Thr Gln Thr Val Leu Leu Lys Thr His Ala Leu Thr Leu Leu
65 70 75 80
Lys Gln Pro Thr Ile Thr Ile Ala Thr Met Pro Asp Leu Ser Val Tyr
85 90 95
Gln Glu Lys Ala Lys Gln His Ala Gln Tyr Trp Leu Asp Thr Val Gln
100 105 110
Pro Ser Phe Ile Asp Ala Asn Gln Gln Leu Ile Asp Phe Gln Gln Lys
115 120 125
Phe Gln Asn Tyr Tyr Lys Lys Leu Ile Glu Leu Ser Gln Lys Ile Asn
130 135 140
Thr Asp Ala Thr Ala Arg Gln Glu Phe Ile Ile Gly Ile Thr Lys Leu
145 150 155 160
Gln Ala Ser Leu Ala Asn His Gln Asn Gln Ile Gln Thr Met Ser Pro
165 170 175
Asn Leu Gln Lys Phe Gln Glu Lys Phe Gly Thr Asp Ala Gln Asn Phe
180 185 190
Ile Asp Ala Ser Lys Thr Ala Gln Lys Ser Leu Ala Asp Lys Asp Gly
195 200 205
Glu Val Ala Gln Leu Thr Gln Arg Ile Glu Thr Leu Asp Ser Asp Ile
210 215 220
Thr Ala Gln Ile Gly Pro Ile Val Ser Gly Thr Ile Ser Met Ile Ala
225 230 235 240
Gly Ile Gly Ser Ile Thr Phe Gly Thr Ile Met Leu Phe Thr Thr Gly
245 250 255
Gly Thr Thr Ala Thr Phe Val Val Pro Met Leu Ala Gly Ile Val Ala
260 265 270
Gly Thr Gly Gly Leu Ile Gly Gly Ser Val Thr Val Gly Phe Ala Ala
275 280 285
Lys Lys Leu Glu Asp Lys Arg Lys Glu Leu Gln Ala Val Thr Glu Lys
290 295 300
Leu Thr Ala Ala Lys Ala Asp Ala Val Ala Leu Ser Leu Leu Ala Gly
305 310 315 320
Gln Leu Asn Val Phe Lys Asp Thr Val Ala Lys Gly Lys Glu Ser Val
325 330 335
Glu Ser Phe Asp Asp Asn Trp Asn Thr Leu Gln Gln Asn Phe Ile Glu
340 345 350
Leu Gln Asn Asn Val Lys Gln Ile Asn Pro Asp Ser Ala Val Leu Gln
355 360 365
Asn Arg Leu Ala Lys Ile Lys Lys Ser Val Asp Asp Leu Ala Val Gln
370 375 380
Ala Lys Gln Gln Glu Lys Val Ile Thr Ala Ile Ser Tyr Gln
385 390 395
<210> 104
<211> 289
<212> БЕЛОК
<213> Bacillus sp.
<400> 104
Met Asn Tyr Val Ala Phe Ser Thr Arg Glu Leu Gly Gly Lys Ser Ser
1 5 10 15
Pro Phe Ile Leu Ser Val Ala Glu Trp Arg Ala Phe Ser Asp Tyr Met
20 25 30
Lys Glu Cys Ile Ser Leu Pro Thr Ser Ile Ser Val Leu Gln Glu Arg
35 40 45
Ile Cys Asn Ile Asn Asp Gly Gln Leu Arg Glu Gly Trp Asn Arg Leu
50 55 60
Leu Thr Gly Asp Ala Val Tyr Pro Met Met Val Asn Thr Gly Met Glu
65 70 75 80
Ile Thr Thr Asn Val Lys Gln Ala Ser Arg Phe Trp Gly Thr Gly Gly
85 90 95
Arg Thr Glu Ile Ile Ser Leu Ala Gln Asn Ile Val Thr Tyr Ala Asp
100 105 110
Leu Ile Cys Ile Lys His His Asp Asp Leu Asn Arg Val Leu Leu Glu
115 120 125
Ala Gln Asn Gly Asn Leu Ser Pro Ser Thr Lys Ser Lys Phe Ile Asp
130 135 140
Ile Cys Asn Asp Leu Ser Glu His Ala Leu Arg Tyr His Gln Gln Ala
145 150 155 160
Gln Thr Met Met Thr Tyr Leu Ser Glu Phe Tyr Thr Glu Ile Glu Gly
165 170 175
Cys Lys Asp Ala His Asp Asn Cys Thr Ala Met Ile Ser Arg Ile Gly
180 185 190
Met Arg Asn His Leu Glu Tyr Ala Val Val His Asn Thr Val Thr Phe
195 200 205
Leu Leu Gly Ala Ile Arg Arg Ile Ile Pro Ser Glu Thr Lys Ile Leu
210 215 220
Pro Val Ile Arg Lys Ile His Leu Ile Trp Gly Ala Ile Ser Tyr Asp
225 230 235 240
Leu Lys Glu Leu Gly Asp Asp Ile Gly Gln Asp Leu Asp Asn Leu Pro
245 250 255
Ala Ile Ile Ala Ala Leu Glu Leu Glu Leu Ala Phe Glu Asn Trp Ser
260 265 270
Ala Ile Arg Gln Glu Ala Glu Asp Phe Tyr Lys Asn Ala Glu Asn Phe
275 280 285
Ser
<210> 105
<211> 535
<212> БЕЛОК
<213> Bacillus sp.
<400> 105
Leu Leu Glu Tyr Val Val Glu Phe Arg Lys Lys Phe Leu Leu Ile Ile
1 5 10 15
Ile Ser Lys Lys His Leu Val Tyr Ser Tyr Leu Phe Asp Tyr Asn Phe
20 25 30
Ile Gln Lys Gly Asp Ser Thr Met Lys Tyr Lys Asp Arg Ile Gln Ala
35 40 45
Lys Arg Asn Tyr Lys Gln Thr Ile Phe Ala Ala Ala Thr Thr Leu Thr
50 55 60
Leu Gly Val Ser Ala Leu Ala Gly Pro Ala Ser Ala Phe Ala Ala Glu
65 70 75 80
Gln Lys Thr Ala Asp His Thr Lys Thr Val Gln Ser Gln Lys Gln Ser
85 90 95
Gln Ile Lys Val Met Ala Asn Asp Asp Ser Tyr Ile Asp Ser Leu Glu
100 105 110
Lys Phe Lys Lys Asn Phe Thr Pro Thr Ser Thr Thr Gly Lys Phe Leu
115 120 125
Asp Asp Leu Thr Lys Lys Gly Leu Gly Ser Leu Thr Lys Ser Ala Asn
130 135 140
Gly Gly Asn Ile Asn Tyr Asn Asn Phe Leu Arg Asp Leu Thr Ile Thr
145 150 155 160
Ser Met Asp Leu Leu Pro Tyr Gly Gly Ala Leu Phe Ser Thr Phe Ile
165 170 175
Ser Leu Met Trp Pro Met Glu Gln Asn Asn Asn Ile Met Asp Gln Leu
180 185 190
Val Glu Lys Phe Ser Lys Leu Val Asp Glu Lys Ile Val Asp Tyr Asp
195 200 205
Asn Gly Thr Leu Lys Gln Lys Ser Ile Ala Leu Gln Asp Gln Leu Lys
210 215 220
Gln Phe Asp Thr Leu Val Asn Arg Ile Glu Asn Lys Val Thr Thr Tyr
225 230 235 240
Met Ser Asp Arg Gln Ala Ala Ala Thr Leu Ala Leu Ser Leu Asn Thr
245 250 255
Ala Phe Asn Asp Leu Leu Arg Glu Cys Gln Lys Asp Asp Gln Lys Val
260 265 270
Ser Glu Leu Pro Leu Tyr Thr Gln Phe Ala Thr Ala His Leu Val Phe
275 280 285
Leu Lys Tyr Val Val Asp Asn Gly Gly Lys Gly Thr Thr Leu Asp Phe
290 295 300
Glu Lys Asn Ala Leu Gln Thr Phe Lys Asp Ser Leu Asp Asn Gly Thr
305 310 315 320
Lys Glu Tyr Ser Glu His Ile Lys Asn Val Ala Tyr Glu Gly Leu Ala
325 330 335
Lys Ile Ser Lys Lys Glu Leu Gly Phe Val Ala Thr Asn Asp Asp Glu
340 345 350
Tyr Thr Gln Ala Met Ala Glu Gly Ala Arg Arg Thr Ile Val Ser Leu
355 360 365
Glu Gly Arg Pro Leu Pro Pro Glu Phe Lys Arg Gln Leu Asp Gln Ala
370 375 380
Arg Ser Asp Leu Asn Ala Ile Gln Asn Gly Lys Lys Tyr Ala Leu Pro
385 390 395 400
Thr Asn Val Ala Glu Lys Leu Asn Pro Tyr Arg Ala Ser Thr Val Asp
405 410 415
Asn Leu Ala Phe Asn Thr Leu Leu Ser Leu Leu Lys Gly Asn Lys His
420 425 430
Thr Gly Trp Ile Gln Glu Asn Gly Arg Tyr Phe Tyr Glu Lys Glu Gly
435 440 445
Ile Arg Asp Thr Ser Gly Trp Tyr Lys Asp Gly Asn Arg Tyr Tyr Tyr
450 455 460
Leu Asp Pro Thr Asp Gly His Met Thr Thr Gly Trp Leu Lys Asp Asp
465 470 475 480
Gly Lys Trp Tyr Tyr Leu Ser Pro Thr Glu Gln Asn Gly Phe Lys Gln
485 490 495
Gly Gln Met Ile Phe Gln Pro Lys Ala Glu Gly Asp Leu Ser Pro Ala
500 505 510
Gln Ile Val Ile Asp Gly Lys Gly Tyr Ser Phe Gly Pro Asp Gly Val
515 520 525
Cys Leu Asn Pro Asp Gly Asn
530 535
<210> 106
<211> 563
<212> БЕЛОК
<213> Bacillus sp.
<400> 106
Leu Lys Asn Asn Pro Asn Gly Lys Glu Tyr Leu Cys Met Asn Tyr Lys
1 5 10 15
Lys Arg Thr Asn Ala Lys Arg Lys Tyr Lys Gln Thr Leu Phe Val Thr
20 25 30
Ile Ala Thr Met Thr Leu Gly Gly Ser Ile Leu Gly Asn Thr Ala Ser
35 40 45
Ala Phe Ala Ala Glu Asn Thr Thr Glu Lys Gln Lys Val Ser Ser Ser
50 55 60
Pro Lys Asn Ser Asp Thr Val Gly Ser Asn Val Phe Lys Val Ala Ala
65 70 75 80
Asp Gly Ser Val Thr Leu Leu Asn Ser Thr Pro Phe Asp Ser Pro Thr
85 90 95
Val Lys Ala Ile Thr Leu Leu Gly Gly Asn Leu Leu Asn Gln Ala Tyr
100 105 110
Leu Asp Ala Tyr Asn Lys Asp Phe Thr Gly Thr Ala Arg Thr Leu Val
115 120 125
Leu Gly Ala Thr Ala Leu Ile Pro Tyr Val Gly Ser Phe Ile Ser Pro
130 135 140
Ile Ile Gly Leu Leu Trp Pro Ala Asn Val Gly Thr Asp Met Thr Thr
145 150 155 160
Gln Thr Lys Ala Leu Val Ala Leu Ile Asn Glu Gln Ile Ala Asp Tyr
165 170 175
Asp Ile Glu Thr Leu Glu Gln Lys Gly Lys Thr Leu Lys Asp Leu Met
180 185 190
Ser Arg Phe Asp Asp Leu Leu Asn Gly Lys Pro Leu Thr Phe Gly Glu
195 200 205
Gln Gly Thr Val Gln Glu Thr Leu Arg Asn Lys Ala Ser Asn Ile Asn
210 215 220
Asp Lys Phe Ile Glu Val Leu Asn Asp Cys Gln Lys Arg Ser Phe Lys
225 230 235 240
Ala Glu Glu Leu Pro Ile Tyr Thr Leu Ile Ala Thr Ala His Leu Leu
245 250 255
Phe Leu Asn Met Ile Glu Gln Asn Gly Gln Ser Ser Arg Ile Gln Met
260 265 270
Asp Asp Glu Thr Tyr Lys Gly Tyr Val Glu Asp Phe Lys Arg Leu Pro
275 280 285
Ile Glu Tyr Ala Asn Tyr Ile Glu Ala Thr Ser Lys Leu Gly Lys Gln
290 295 300
Lys Phe Asn Lys Lys Met Asp Asp Thr Ile Asn Ala Ala Lys Lys Gln
305 310 315 320
Ala Leu Val Gly Ile Ile Ser Pro Asp Glu Asn Thr Asn Leu Glu Asn
325 330 335
Leu Glu Lys Thr Arg Lys Gln Cys Leu Asp Glu Ala Lys Leu Leu Gln
340 345 350
Gln Met Gly Asp Ala Tyr Gly Ala Glu Lys Ala Arg Gln Lys Ala Gln
355 360 365
Lys Ala Tyr Glu Tyr Lys Lys Gln Tyr Glu Asp Leu Ile Gln Lys Arg
370 375 380
Gly Asn Tyr Ile Lys Asn Thr Ala Asp Asn Glu Ala Phe Lys Leu Ala
385 390 395 400
Lys Lys Ser Met Glu Glu Leu Met Ile Asp Val Arg Ile Pro Asn Gly
405 410 415
Lys Tyr Ile Ile Gln Ser Val Gln Asn Ser Asn Met Val Val Gly Met
420 425 430
Ser Gly Asn Asn Ile Gln Leu Glu Glu Asn Asn Gly Gln Leu Asn Asn
435 440 445
Lys Phe Phe Ile Ile Tyr Asp Gln Ala Arg Asn Ser Tyr Val Ile Glu
450 455 460
Thr Ser Val Glu Thr Gly Leu Ser Ser Tyr Met Ala Ala Asn Ile Asp
465 470 475 480
Tyr Ser Asn Val Asn Lys Ser Gly Val Asn Val Tyr Ala Ile Leu Ser
485 490 495
Thr Ser Ser His Glu His Pro Asn Leu Leu His Trp Lys Leu Lys Arg
500 505 510
Glu Arg Gly Ser Lys Phe Val Tyr Leu Glu Asn Leu Glu Asn Gly Gln
515 520 525
Val Leu Asp Val Ser Asn Ala Gly Thr Glu Lys Gly Thr Asn Ile Ile
530 535 540
Thr Trp Pro Leu Lys Gln Gln Gly Gln Asn Gln Arg Phe Glu Leu Val
545 550 555 560
Pro Thr Asn
<210> 107
<211> 393
<212> БЕЛОК
<213> Pseudomonas sp.
<400> 107
Met Leu Ala Ile Gln Glu Gln Ile Ile Thr Ala Lys Gln Ile Asn Ser
1 5 10 15
Leu Thr Ser Asp Phe Ile Gly Ala Ala Ser Gly Thr Leu Glu Gly Val
20 25 30
Glu Arg Glu Gln Gly Leu Leu Val Thr Asn Asp Asp Ile Arg Lys Ile
35 40 45
Lys Arg Tyr Val Asn Thr Ala Leu Thr Leu Pro Ile Glu Thr Asn Glu
50 55 60
Ile Glu Gln Ile His Lys Tyr Asp Gln Leu Asn Ile Ala Gly Leu Lys
65 70 75 80
Ser Ala Asp Met Gln Ile Leu Phe Lys Ser Met Arg Asn His Ala Ala
85 90 95
Ser Trp Ser Pro Leu Glu Ser Asn Met Lys Gln Val Gly Ser Asp Leu
100 105 110
His Val Phe Ala Asp Asn Phe Thr Ser Ser Ser Gln Ser Ile Ile Asn
115 120 125
Tyr Leu Glu Arg Leu Pro Ser Tyr Thr Ser Gly Ile Gly Lys Val Gly
130 135 140
Asp Leu Ser Pro Glu Glu Ile Asp Asn Leu Pro Glu Ile Gln Leu Thr
145 150 155 160
Glu Gly Glu Lys Arg Lys Ile Pro Ala Leu Leu Glu Leu Val Glu Asp
165 170 175
Leu Lys Thr Val Ile Thr Gln His Ser Ala Ser Thr Gln Thr Thr Lys
180 185 190
Thr Arg Ile Ser Glu Phe Lys Arg Glu Ile Ser Asn Ser Leu Lys Pro
195 200 205
Ala Leu Gly Leu Lys Ile Ala Leu Cys Asn Ser His Asn Phe Asp Glu
210 215 220
Glu Ile Lys Ser Phe Asn Glu Lys Leu Glu Ile Leu Asn Gln Arg Ile
225 230 235 240
Asn Glu Lys Leu Ala Glu Ile Glu Glu Tyr Ser Lys Ser Lys Trp Trp
245 250 255
Gly Leu Ile Gly Gly Ala Phe Gly Phe Ile Ile Ser Ser Ser Ile Tyr
260 265 270
Gly Ala Lys Ala Asn Glu Ala Arg Lys Ala Leu Asp Arg Leu Thr Thr
275 280 285
Glu Arg Arg Glu Ile Glu Thr Arg Ile Ala Thr Thr Asn Ala Ile Leu
290 295 300
Ala Ser Leu Arg Ala Phe Glu Thr Asn Leu Gln Asp Leu His Val Arg
305 310 315 320
Ile Glu Gly Ala Ala Gly Ser Ser Ser Asn Leu Glu Ser Leu Trp Glu
325 330 335
Leu Ile Gln Ala Tyr Ile Glu Ser Ser Ser Lys Arg Leu Asp Gly Val
340 345 350
Thr Asn Ala Met Tyr Leu Val Ser Phe Val Ser Arg Leu Thr Thr Met
355 360 365
Met Asp Asn Trp Ser Glu Ile Lys Lys Gln Ala Gly Asp Leu Leu Thr
370 375 380
Ala Phe Asn Asn Ala Thr Ser Asp Ala
385 390
<210> 108
<211> 439
<212> БЕЛОК
<213> Bacillus sp.
<400> 108
Met Lys Asn Arg Phe Ile Asn Gly Phe Leu Val Thr Ser Leu Leu Thr
1 5 10 15
Gly Gly Ala Ile Pro Ile Cys Thr Leu Ala Thr Pro Val Ala Leu Ala
20 25 30
Glu Thr Gln Gln Asp Asn Leu Asp Ile Ser Leu Ser Leu Ser Lys Leu
35 40 45
Gly Arg Gln Ser Gln Phe Ile Gln Leu Cys Val Asp Asp Ala Leu Lys
50 55 60
Arg Pro Asn Ile Gln Met Thr Glu Leu Pro Ala Leu Gln Thr Asp Gln
65 70 75 80
Ser Leu Ile Lys Gln Asp Met Gln Glu Trp Ser Ser Glu Leu Tyr Pro
85 90 95
His Leu Val Leu Ile Asn Ala Lys Ser Lys Gly Phe Thr Thr Lys Phe
100 105 110
Asn Ser Tyr Tyr Pro Asp Leu Lys Gln Phe Val Asp Ser Asn Glu Asp
115 120 125
Gln Gln Gly Phe Leu Asp Arg Leu Glu Ala Leu Gln Asp Val Val Ser
130 135 140
Ser Asn Gln Gly Lys Ile Gln Ser His Ile Asn Glu Leu Gln Ser Phe
145 150 155 160
Gln Ser Arg Leu Asp Asn Asp Met Lys Lys Leu Gly Asp His Ala Lys
165 170 175
Glu Gly Lys Asp Leu Leu Ser Gly Ser Gly Ser Gly Lys Ile Asp Gln
180 185 190
Tyr Asn Met Ala Leu Ser Glu Ala Arg Asn Lys Ile Gln Lys Asn Leu
195 200 205
Gln Glu Ile Ala Leu Leu Pro Gly Ala Leu Asn Ala Lys Gly Phe Asp
210 215 220
Ile Phe Lys Glu Val Tyr Asn Leu Ser Lys Glu Leu Met Thr Pro Val
225 230 235 240
Ala Glu Ser Ala Ile Ala Ala Val Asn Lys Gly Lys Glu Ile Glu Lys
245 250 255
Ser Ile Val Glu Ala Glu Asn Ser Ala Glu Lys Val Ala Lys Glu Ala
260 265 270
Asn Lys Ser Thr Ala Glu Ile Glu Thr Ala Lys Lys Glu Ala Arg Glu
275 280 285
Lys Ile Glu Lys Asp Lys Lys Asn Glu Leu Ala Ala Ala Ala Ala Ala
290 295 300
Lys Ala Gln Glu Tyr Asp Leu Leu Gln Met Ile Asp Ile Asp Lys Ile
305 310 315 320
Gln Lys Thr Tyr Asn Ser Phe Ala Glu Val Asn Lys Leu Ser Ala Ser
325 330 335
Gln Arg Ala Tyr Leu Glu Asp Leu Gln Lys Gln Asn Glu Thr Ile Tyr
340 345 350
Lys Leu Thr Thr Lys Leu Thr Ile Val Asp Ile Gln Lys Asp Leu Leu
355 360 365
Asn Leu Met Lys Asn Asp Ala Asp Thr Phe Thr Asn Gln Val Lys Leu
370 375 380
Glu Ile Ser Leu Leu Glu Asn Tyr Lys Lys Asp Trp Asp Gln Val Lys
385 390 395 400
Asp Cys Ile Thr Gln Leu Ser Thr Arg Ile Ser Asp Pro Lys Val Gln
405 410 415
Ser Ala Gln Leu Lys Arg Leu Lys Asp Leu Asn Ser Gln Leu Glu Glu
420 425 430
Gln Met Asn Gly Phe Asn Ser
435
<210> 109
<211> 954
<212> БЕЛОК
<213> Bacillus sp.
<400> 109
Met Ile Met Gln Met Val Thr Lys Ala Met Thr Pro Lys Leu Gly Ala
1 5 10 15
Leu Pro Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Asp
20 25 30
Asn Ile Thr Gly Val Met Ser Thr Ile Phe Gly Val Asp Thr Gly Asp
35 40 45
Ser Ser Ile Asp Asp Val Leu Asn Asn Glu Glu Leu Leu Lys Glu Met
50 55 60
Gln Ala Gln Met Gln Gln Met Gln Gln Ser Ile Thr Asp Met Ala Asn
65 70 75 80
Asp Gln Ile Ile Ser Glu Gly Ile Glu Asn Gln Ile Leu Ala Leu Thr
85 90 95
Thr Asp Leu Ser Thr Ser Ile Thr Ala Glu Phe Asn Lys Ile Glu Gly
100 105 110
Ile Leu Asn Thr Tyr Leu Pro Ala Ile Ser Asn Met Leu Gly Lys Val
115 120 125
Tyr Glu Gln Thr Ser Glu Ile Asn Glu Lys Val Asp Lys Leu Leu Ala
130 135 140
Met Met Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val
145 150 155 160
Val Leu Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu
165 170 175
Val Tyr Val Asn Lys Thr Phe Leu Ala Leu Thr Lys Ser Phe Leu Gln
180 185 190
Asn Glu Asp Leu Ser Ile Glu Asn Met Gln Glu Met Leu Glu Trp Ala
195 200 205
Lys Ser Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp
210 215 220
Thr Leu His Thr Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala
225 230 235 240
Leu Lys Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asn Gln Tyr Gly
245 250 255
Asp Phe Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu
260 265 270
Ala Thr Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys
275 280 285
Val Leu Gly Leu Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg
290 295 300
Ile Glu Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn
305 310 315 320
Tyr Ser Asn Val Leu Glu Ile Glu Gly Ile Tyr Pro Met Pro Gln Gln
325 330 335
Ser Gly Asp Cys Asn Ser Leu Asp Leu Gln Ala Lys Ser Gly Tyr Ala
340 345 350
Leu Ile Gly Leu Glu Phe Phe Met Asp Asn Gly Ile Tyr Lys Ala Lys
355 360 365
Ala Tyr Gln Ala Glu Leu Asp Lys Asn Phe Ser Ile His Ala Asp Thr
370 375 380
Val Lys Glu Ile Ile Ser Asp Asp Leu Ser Lys Val Phe Leu Asn Gln
385 390 395 400
Thr Asn Thr Ala Glu Asp Tyr Met Tyr Pro Leu Phe Gly Glu Leu Lys
405 410 415
Gly Val Pro Asn Thr Ile Ile Thr Arg Ile Gly Phe Gly Thr Lys Tyr
420 425 430
Asp Glu Ser Arg Lys Val Lys Ala Phe Ala Tyr Ile Asp Ala Asp Phe
435 440 445
Ser Pro Tyr Asp Pro Lys Ser Gly Thr Ile Ser Lys Glu Gly Thr Gln
450 455 460
Thr Phe Gln Ile Glu Gly Thr Glu Asn Lys Asn Trp Cys Tyr Asn Ala
465 470 475 480
Trp Pro Ile Gly Leu Ile Gly Asp Leu Tyr Met Thr Pro Leu Lys Ser
485 490 495
Leu Ser Leu Asn Val Asp Val Asp Ile His Ala Gly Asn Thr Leu Asn
500 505 510
Met Ser Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser Gly Glu Tyr Asn
515 520 525
Ala Asn Phe Ile Leu Phe Pro Tyr Thr Asn Ser Ser Asn Pro Ile Val
530 535 540
Glu Asn Leu Ile Gln Asn Gly Asp Phe Glu Asp Glu Asp Lys His Trp
545 550 555 560
Glu Val Asn Gly His Thr Ala Val Ile Ala Lys Gly Glu Gly Ile Asn
565 570 575
Gly Ser Lys Ala Met Lys Leu Glu Asn Gly Thr Ile Glu Gln Lys Leu
580 585 590
Asn Leu Lys Pro Tyr Thr Asn Tyr Glu Phe Thr Ala Tyr Val Lys Gly
595 600 605
Lys Ser Ala Arg Phe Leu Ile Arg Asp Asn Thr Leu Ala Asp Phe Ala
610 615 620
Glu Gly Tyr Leu Asp Ser Thr Ile Tyr Lys Gln Ile Lys Phe Arg Phe
625 630 635 640
Lys Thr Ala Met Asp Ala Ser Ser Phe Ile Val Ser Ala Thr Thr Pro
645 650 655
Gln Glu Ile Leu Val Asp Asn Ile Lys Val Cys Glu Val Thr Glu Thr
660 665 670
Asp Asn Leu Ile Asp Cys Gly Asp Phe Asp Phe Tyr Glu Ile Asn Glu
675 680 685
Glu Asp Leu Lys Ile Arg Leu Tyr Pro Asn His Trp Glu Arg Tyr Gly
690 695 700
Gly Trp His Ile Leu Ser Gly Lys Gly Met Phe Asp Ser Glu Ala Leu
705 710 715 720
Thr Ile Ile Asn Ser Gly Gly Ala Asn Gln Lys Val Gln Leu Lys Ala
725 730 735
Asn Thr Asn Tyr Ile Leu Thr Ala Tyr Val Lys Val Asp Asn Pro Asn
740 745 750
Thr Thr Ile Gln Ile Gly Cys Gly Ser Asn Gln Leu Thr Cys Asn Ser
755 760 765
Thr Ser Tyr Thr Pro Val Lys Leu Lys Phe Lys Thr Gly Glu Asp Pro
770 775 780
Ala Thr Thr Glu Asn Phe Ile Tyr Cys Ser Asn Pro Asn Asp Ser Gly
785 790 795 800
Thr Val Trp Ala Asp Asn Phe Leu Leu Cys Glu Val Pro Asn Leu Ile
805 810 815
Val Asn Gly Asn Phe Glu Gln Met Asp Leu Ser Ser Trp Ser Leu Ser
820 825 830
Thr Thr Asp Val Gly Lys Ile Tyr Leu Gln Ser Gly Arg Gly Met Ser
835 840 845
Asn Ser Asn Ala Ile Val Leu Ala Gly Glu Gly Gln Ile Ser Gln Lys
850 855 860
Val Pro Leu Lys Pro Tyr Thr Lys Tyr Arg Leu Thr Ala Tyr Val Arg
865 870 875 880
Val Pro Asn Gly Val Thr Ala Leu Leu Gly Tyr Gly Asn Gly Gly Asn
885 890 895
Asp Asp Tyr Arg Val His Cys Thr Ser Thr Glu Phe Lys Gln Glu Ser
900 905 910
Leu Val Phe Thr Thr Gly Ala Asn Pro Leu Gln Ser Glu Asp Val Ile
915 920 925
Tyr Leu Ser Thr Pro Asp Asp Gly Phe Lys Ile Thr Thr Gly Asp Asn
930 935 940
Phe Glu Leu Tyr Glu Leu Asp Gln Ile Glu
945 950
<210> 110
<211> 407
<212> БЕЛОК
<213> Bacillus sp.
<400> 110
Leu Glu Arg Arg Asn Met Arg Lys Gln Pro Tyr Lys Val Met Ala Leu
1 5 10 15
Ser Thr Leu Met Ala Val Phe Ala Ala Ser Asn Ile Met Pro Ala His
20 25 30
Ser Phe Ala Ala Glu Ser Thr Val Lys Pro Val Pro Ile His Ser Met
35 40 45
Glu Lys Ala Asn Asn Lys Asp Asp Gly Tyr Ser Leu Gly Pro Glu Gly
50 55 60
Leu Lys Asp Ala Ile Glu Arg Thr Gly Ser Asn Ala Leu Val Met Asp
65 70 75 80
Leu Tyr Ala Leu Thr Ile Ile Lys Gln Gly Asn Ala Asn Phe Ser Asn
85 90 95
Val Thr Ser Val Asp Lys Ser Leu Lys Glu Lys Val Ile Lys His Gln
100 105 110
Asn Asp Ala Arg Thr Asn Ala Lys Gln Trp Leu Asp Val Met Lys Pro
115 120 125
Gln Ile Ile Ser Thr Asn Gln Asp Ile Ile Asn Tyr Asp Thr Lys Phe
130 135 140
Gln Asn Tyr Tyr Asp Thr Leu Val Lys Ala Val Lys Asp Lys Asp Lys
145 150 155 160
Asp Thr Leu Thr Lys Gly Leu Thr Arg Leu Ser Asn Ser Ile Thr Gly
165 170 175
Asn Lys Glu Lys Val Asp His Leu Val Glu Asp Leu Lys Lys Phe Arg
180 185 190
Ser Lys Met Thr Asp Asp Thr Gln Asn Phe Lys Asn Asp Ala Asn Lys
195 200 205
Ile Thr Ser Ile Leu Ala Ser Gln Asp Ala Gly Ile Pro Leu Leu Gln
210 215 220
Asn Gln Leu Ser Ala Tyr Asn Glu Ala Val Ser Lys Tyr Asn Ala Ile
225 230 235 240
Ile Ile Gly Ser Ser Val Ala Val Ala Leu Gly Pro Val Ala Ile Val
245 250 255
Gly Gly Thr Val Leu Leu Ala Thr Gly Gly Gly Thr Val Leu Gly Val
260 265 270
Gly Leu Ile Ala Ser Gly Ile Gly Gly Ile Ala Gly Gly Thr Thr Gly
275 280 285
Leu Ile Leu Ala Lys Lys Glu Leu Asp Asn Ala Gln Ser Glu Ile Lys
290 295 300
Asn Ile Thr Gly Gln Ile Thr Thr Ala Gln Leu Glu Val Ala Gly Leu
305 310 315 320
Thr Asn Ile Lys Ala Gln Thr Glu Tyr Leu Thr Asn Thr Ile Asp Ile
325 330 335
Ala Ile Thr Ala Leu Gln Asn Ile Ser Asn Gln Trp His Thr Met Gly
340 345 350
Ala Lys Tyr Gly Ala Leu Leu Glu Asn Val Asn Thr Ile Ser Pro Glu
355 360 365
Asp Leu Ala Phe Ile Glu Glu Asp Leu Ser Val Ala Lys Asp Ser Trp
370 375 380
Lys Asn Val Lys Asp Tyr Ala Glu Lys Ile Tyr Ala Glu Asp Ile Lys
385 390 395 400
Val Val Asp Asn Lys Arg Ile
405
<210> 111
<211> 209
<212> БЕЛОК
<213> Bacillus sp.
<400> 111
Leu Asn Thr Asp Thr Asn Lys Asn Ala Ile Tyr Tyr Ala Gly Asn Asp
1 5 10 15
Ile Tyr Ala Thr Cys Gly Asn Trp Ala Asn His Ile Gln His Ser Phe
20 25 30
Glu Ser Leu Ile Asp Arg Ala Ser Gly Asn His Glu Val Ala Ser Leu
35 40 45
Pro Leu Tyr Thr Lys Leu Ala Val Ala His Ile Thr Phe Leu Lys Tyr
50 55 60
Met His Glu His Ala Lys Gly Pro Lys Leu Lys Tyr Asp Glu Gln Ser
65 70 75 80
Leu Gln His Phe Phe Asp Lys Gly Asn Leu Glu Val Ile Thr Lys Lys
85 90 95
Tyr Ala Asp His Ile Lys Lys Val Tyr Asn Gln Gly Leu Gln Thr Phe
100 105 110
Tyr Asn Asn Ile Asp Lys Leu Asn Glu Leu Arg Thr Lys Lys Glu Asn
115 120 125
Leu Leu Ser Thr Ala Gly Asp Gly Ile Gly Lys Glu Asn Glu Leu Asn
130 135 140
Ile Leu Asn Ala Lys Ile Ala Lys Leu Glu Ala Lys Asn Ile Phe Lys
145 150 155 160
Asn Met Gln Asn Phe Ser Ser Ser Thr Trp Asp Asp Pro Thr Phe Gln
165 170 175
Ala Leu Ile Gln Gly Glu Trp Lys Leu Glu Asn Asn Ala Trp Thr Phe
180 185 190
Thr Asn Lys Lys Gly Glu Lys Lys Thr Gly Trp Leu Glu Leu Gly Ile
195 200 205
Gly
<210> 112
<211> 945
<212> БЕЛОК
<213> Bacillus sp.
<400> 112
Met Ile Met Gln Met Val Thr Lys Ala Met Thr Pro Lys Leu Gly Ala
1 5 10 15
Leu Pro Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Gly
20 25 30
Asn Ile Thr Asp Val Met Ser Thr Ile Phe Gly Ile Asp Thr Gly Asp
35 40 45
Ser Ser Ile Asp Asp Val Leu Asn Asn Glu Glu Leu Leu Gln Glu Met
50 55 60
Leu Asp Gln Met Asn Thr Met Gln Ile Ser Ile Arg Asp Ile Leu Glu
65 70 75 80
Gln Gln Gly Ile Ser Ala Asp Ile Glu Lys Gln Ile Leu Ala Leu Thr
85 90 95
Thr Asp Leu Ala Thr Ser Ile Asn Thr Glu Leu Gly Lys Ile Glu Gly
100 105 110
Ile Leu Asn Thr Tyr Leu Pro Ala Ile Ser Asn Met Leu Ser Asn Ile
115 120 125
Tyr Glu Gln Thr Ser Val Ile Asp Gln Lys Val Asp Lys Leu Leu Ala
130 135 140
Leu Met Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val
145 150 155 160
Val Leu Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Glu Lys Leu
165 170 175
Val Tyr Val Asn Lys Lys Phe Leu Ser Leu Thr Lys Gly Phe Leu Gln
180 185 190
Gly Glu Asp Val Ser Ile Asp Ser Met Gln Glu Ile Gln Glu Trp Ala
195 200 205
Arg Ser Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp
210 215 220
Thr Leu His Asn Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala
225 230 235 240
Leu Lys Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly
245 250 255
Asp Phe Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu
260 265 270
Ala Thr Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys
275 280 285
Val Leu Gly Leu Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg
290 295 300
Ile Glu Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn
305 310 315 320
Tyr Ser Asn Ala Leu Glu Ile Thr Gly Ile Tyr Pro Met Ser Val Asp
325 330 335
Trp Gly Asp Cys Lys Ser Thr Asp Leu Gln Ala Asp Ala Gly Tyr Ala
340 345 350
Leu Ile Gly Leu Glu Phe Phe Met Asp Asn Gly Ile Tyr Lys Ala Lys
355 360 365
Ala Tyr Gln Gly Lys Met Gly Lys Asn Phe Ser Val Ser Ala Asp Thr
370 375 380
Val Thr Glu Ile Ile Ser Asp Asp Leu Ser Thr Val Phe Asn Asp Met
385 390 395 400
Thr Asn Asp Asn Pro Glu Leu Tyr Val Val Tyr Pro Leu Ser Gly Glu
405 410 415
Leu Thr Gly Pro Pro Asn Thr Ile Ile Thr Arg Ile Gly Phe Gly Thr
420 425 430
Lys Tyr Asp Lys Thr Arg Glu Ser Ser Val Gln Ala Phe Ala Tyr Val
435 440 445
Asp Ala Asp Phe Ser Pro Tyr Asp Tyr Ile Ser Gly Thr Ile Ser Lys
450 455 460
Glu Gly Thr Gln Thr Val Ser Leu Glu Gly Asn Asp His Lys Asn Arg
465 470 475 480
Cys Tyr Ser Met Trp Pro Met Gly Leu Ile Gly Asp Leu Tyr Met Thr
485 490 495
Pro Leu Lys Ser Leu Ser Leu Asn Val Glu Asp Thr Gly Thr Thr Leu
500 505 510
Asn Met Ser Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser Arg Glu Tyr
515 520 525
Asn Thr Asn Phe Ile Leu Phe Pro Tyr Thr Asn Asn Ser Ser Leu Ile
530 535 540
Asp Lys Asn Leu Ile Gln Asn Gly Asn Leu Glu Glu Gln Asp Lys Tyr
545 550 555 560
Trp Glu Gly Thr Glu Pro Ser Phe Phe Ala Glu Gly Glu Gly Thr Phe
565 570 575
Gly Ser Asn Ala Leu Lys Val Gln Ala Gln Gly Ala Phe Asp Gln Ile
580 585 590
Val Lys Leu Glu Pro Asn Thr Thr Tyr Arg Leu Thr Ala Phe Thr Lys
595 600 605
Val Lys Asp Ser Asn Tyr Ser Lys Gly Arg Ile Gly Ile Arg Asp Met
610 615 620
Tyr Asn Tyr Arg Val Glu Glu Ser Phe Leu His Thr Arg Tyr Asn Gln
625 630 635 640
Thr Lys Val Glu Phe Thr Thr Gly Ala Asp Thr Ser Lys Leu Ser Val
645 650 655
Phe Ile Gln Gly Gly Gly Asp Gln Glu Ser Ile Ala Trp Ala Asp Asn
660 665 670
Ile Glu Leu Tyr Lys Val His Gln Gln Asp Asn Met Ile Ser Asp Phe
675 680 685
Asp Phe Asp Thr Tyr Lys Tyr Ser Asn Met Cys Trp Lys Leu Ser Asp
690 695 700
Gly Gly Asp Phe Val Glu Lys Glu Gly Leu Phe Asn Ser Lys Ala Leu
705 710 715 720
Lys Ile Thr Asn Ser Gly Gly Ala Glu Gln Glu Val Lys Leu Lys Ala
725 730 735
Asn Thr Asn Tyr Ile Phe Thr Ala Tyr Val Lys Val Asp Asn Thr Thr
740 745 750
Ala Gln Ile Gly Cys Gly Ser Asn Gln Val Thr Cys Asn Ser Thr Ser
755 760 765
Tyr Thr Pro Val Lys Leu Lys Phe Lys Thr Gly Glu Asp Pro Ser Thr
770 775 780
Thr Glu Ser Ser Val Tyr Cys Ser Asn Ser Asn Asp Ile Gly Thr Val
785 790 795 800
Trp Ala Asp Asn Phe Val Leu Tyr Glu Ala Pro Asn Leu Ile Ile Asn
805 810 815
Gly Asp Phe Glu Gln Phe Asp Gln Ser Ser Trp Asn Phe Ser Pro Ser
820 825 830
Glu Gly Gly Ser Ile Tyr Leu Gln Ser Gly Leu Gly Met Ser Arg Ser
835 840 845
Asn Ala Val Val Leu Arg Gly Gln Asn Gly Lys Ile Ser Gln Lys Val
850 855 860
Pro Leu Lys Pro Phe Thr Lys Tyr Arg Leu Thr Ala Tyr Val Arg Val
865 870 875 880
Ser Lys Gly Ser Leu Ala Tyr Ile Gly Tyr Gly Glu Tyr Asn Cys Ala
885 890 895
Cys Ala Val Asp Asp Phe Arg Gln Ala His Val Glu Phe Thr Thr Gly
900 905 910
Ala Asn Pro Met Gln Ser Asp Asp Ala Ile Tyr Leu Asp Ser Phe Gly
915 920 925
Ser Ala Tyr Thr Val Ala Asp Asn Phe Glu Leu Tyr Glu Leu Asp Gln
930 935 940
Ile
945
<210> 113
<211> 943
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 113
Met Gln Met Val Thr Lys Ala Met Thr Pro Lys Leu Gly Ala Leu Pro
1 5 10 15
Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Gly Asn Ile
20 25 30
Thr Asp Val Met Ser Thr Ile Phe Gly Ile Asp Thr Gly Asp Ser Ser
35 40 45
Ile Asp Asp Val Leu Asn Asn Glu Glu Leu Leu Gln Glu Met Leu Asp
50 55 60
Gln Met Asn Thr Met Gln Ile Ser Ile Arg Asp Ile Leu Glu Gln Gln
65 70 75 80
Gly Ile Ser Ala Asp Ile Glu Lys Gln Ile Leu Ala Leu Thr Thr Asp
85 90 95
Leu Ala Thr Ser Ile Asn Thr Glu Leu Gly Lys Ile Glu Gly Ile Leu
100 105 110
Asn Thr Tyr Leu Pro Ala Ile Ser Asn Met Leu Ser Asn Ile Tyr Glu
115 120 125
Gln Thr Ser Val Ile Asp Gln Lys Val Asp Lys Leu Leu Ala Leu Met
130 135 140
Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val Val Leu
145 150 155 160
Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Glu Lys Leu Val Tyr
165 170 175
Val Asn Lys Lys Phe Leu Ser Leu Thr Lys Gly Phe Leu Gln Gly Glu
180 185 190
Asp Val Ser Ile Asp Ser Met Gln Glu Ile Gln Glu Trp Ala Arg Ser
195 200 205
Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp Thr Leu
210 215 220
His Asn Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala Leu Lys
225 230 235 240
Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly Asp Phe
245 250 255
Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu Ala Thr
260 265 270
Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys Val Leu
275 280 285
Gly Leu Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg Ile Glu
290 295 300
Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn Tyr Ser
305 310 315 320
Asn Ala Leu Glu Ile Thr Gly Ile Tyr Pro Met Ser Val Asp Trp Gly
325 330 335
Asp Cys Lys Ser Thr Asp Leu Gln Ala Asp Ala Gly Tyr Ala Leu Ile
340 345 350
Gly Leu Glu Phe Phe Met Asp Asn Gly Ile Tyr Lys Ala Lys Ala Tyr
355 360 365
Gln Gly Lys Met Gly Lys Asn Phe Ser Val Ser Ala Asp Thr Val Thr
370 375 380
Glu Ile Ile Ser Asp Asp Leu Ser Thr Val Phe Asn Asp Met Thr Asn
385 390 395 400
Asp Asn Pro Glu Leu Tyr Val Val Tyr Pro Leu Ser Gly Glu Leu Thr
405 410 415
Gly Pro Pro Asn Thr Ile Ile Thr Arg Ile Gly Phe Gly Thr Lys Tyr
420 425 430
Asp Lys Thr Arg Glu Ser Ser Val Gln Ala Phe Ala Tyr Val Asp Ala
435 440 445
Asp Phe Ser Pro Tyr Asp Tyr Ile Ser Gly Thr Ile Ser Lys Glu Gly
450 455 460
Thr Gln Thr Val Ser Leu Glu Gly Asn Asp His Lys Asn Arg Cys Tyr
465 470 475 480
Ser Met Trp Pro Met Gly Leu Ile Gly Asp Leu Tyr Met Thr Pro Leu
485 490 495
Lys Ser Leu Ser Leu Asn Val Glu Asp Thr Gly Thr Thr Leu Asn Met
500 505 510
Ser Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser Arg Glu Tyr Asn Thr
515 520 525
Asn Phe Ile Leu Phe Pro Tyr Thr Asn Asn Ser Ser Leu Ile Asp Lys
530 535 540
Asn Leu Ile Gln Asn Gly Asn Leu Glu Glu Gln Asp Lys Tyr Trp Glu
545 550 555 560
Gly Thr Glu Pro Ser Phe Phe Ala Glu Gly Glu Gly Thr Phe Gly Ser
565 570 575
Asn Ala Leu Lys Val Gln Ala Gln Gly Ala Phe Asp Gln Ile Val Lys
580 585 590
Leu Glu Pro Asn Thr Thr Tyr Arg Leu Thr Ala Phe Thr Lys Val Lys
595 600 605
Asp Ser Asn Tyr Ser Lys Gly Arg Ile Gly Ile Arg Asp Met Tyr Asn
610 615 620
Tyr Arg Val Glu Glu Ser Phe Leu His Thr Arg Tyr Asn Gln Thr Lys
625 630 635 640
Val Glu Phe Thr Thr Gly Ala Asp Thr Ser Lys Leu Ser Val Phe Ile
645 650 655
Gln Gly Gly Gly Asp Gln Glu Ser Ile Ala Trp Ala Asp Asn Ile Glu
660 665 670
Leu Tyr Lys Val His Gln Gln Asp Asn Met Ile Ser Asp Phe Asp Phe
675 680 685
Asp Thr Tyr Lys Tyr Ser Asn Met Cys Trp Lys Leu Ser Asp Gly Gly
690 695 700
Asp Phe Val Glu Lys Glu Gly Leu Phe Asn Ser Lys Ala Leu Lys Ile
705 710 715 720
Thr Asn Ser Gly Gly Ala Glu Gln Glu Val Lys Leu Lys Ala Asn Thr
725 730 735
Asn Tyr Ile Phe Thr Ala Tyr Val Lys Val Asp Asn Thr Thr Ala Gln
740 745 750
Ile Gly Cys Gly Ser Asn Gln Val Thr Cys Asn Ser Thr Ser Tyr Thr
755 760 765
Pro Val Lys Leu Lys Phe Lys Thr Gly Glu Asp Pro Ser Thr Thr Glu
770 775 780
Ser Ser Val Tyr Cys Ser Asn Ser Asn Asp Ile Gly Thr Val Trp Ala
785 790 795 800
Asp Asn Phe Val Leu Tyr Glu Ala Pro Asn Leu Ile Ile Asn Gly Asp
805 810 815
Phe Glu Gln Phe Asp Gln Ser Ser Trp Asn Phe Ser Pro Ser Glu Gly
820 825 830
Gly Ser Ile Tyr Leu Gln Ser Gly Leu Gly Met Ser Arg Ser Asn Ala
835 840 845
Val Val Leu Arg Gly Gln Asn Gly Lys Ile Ser Gln Lys Val Pro Leu
850 855 860
Lys Pro Phe Thr Lys Tyr Arg Leu Thr Ala Tyr Val Arg Val Ser Lys
865 870 875 880
Gly Ser Leu Ala Tyr Ile Gly Tyr Gly Glu Tyr Asn Cys Ala Cys Ala
885 890 895
Val Asp Asp Phe Arg Gln Ala His Val Glu Phe Thr Thr Gly Ala Asn
900 905 910
Pro Met Gln Ser Asp Asp Ala Ile Tyr Leu Asp Ser Phe Gly Ser Ala
915 920 925
Tyr Thr Val Ala Asp Asn Phe Glu Leu Tyr Glu Leu Asp Gln Ile
930 935 940
<210> 114
<211> 427
<212> БЕЛОК
<213> Pseudomonas sp.
<400> 114
Met Pro Arg Asp Met Thr Ser Pro Lys Pro Pro Thr Ser Ser Phe Ser
1 5 10 15
Thr Pro Thr Thr Leu Arg Val Cys Ala Thr Ser Pro Arg Lys Gly Ala
20 25 30
Ser Ala Gly Ser Ala His Glu Ser Glu Ser Asn Arg Ile Asp Ala Ala
35 40 45
Leu Tyr Arg Arg Gly Ala Ala Ala Ser Asn Arg Ser Arg Ala Met Gly
50 55 60
Asp Lys Asp Gln Leu Leu Ser Pro Gly Glu Leu Ala Ala Val Lys Asp
65 70 75 80
Ser Pro Phe Leu Leu Ser Lys Gly Glu Trp Leu Ser Val Gln Arg Tyr
85 90 95
Val Thr Asn Thr Met Ala Leu Pro Ile Thr Glu Asp Val Met Arg Thr
100 105 110
Gln Leu Phe Met Pro Ala Asp Met Lys Phe Asp Asp Phe Thr Pro Leu
115 120 125
Leu Glu Gly Tyr Lys Thr Val Val Pro His Val Thr Lys Trp Lys Thr
130 135 140
Thr Thr Phe Pro Ala Thr Val Lys Leu Ala Gly Asp Ile Val Leu Tyr
145 150 155 160
Asn Lys Met Ala Lys Ala Tyr Tyr Asn Gly Leu Lys Glu Asn Ile Asp
165 170 175
Leu Leu Leu Lys Asn Pro Asn Asp Glu Lys Ala Arg Leu Glu Leu Ser
180 185 190
Ala Ser Ile Lys Met Leu Ser Gly Met Ala Lys Lys Tyr Ala Asp Arg
195 200 205
Ala Glu Thr Val His Gly Asp Ile Gln Ala Phe Ser Leu Gln Thr Ala
210 215 220
Thr Asp Ala Val Leu Ile Asp Asn Leu Asn Gly Val Tyr Lys Gln Lys
225 230 235 240
Phe Gly Ala Asn Ser Val Trp Gly Lys Lys Leu Val Asp Asp Leu Asn
245 250 255
Glu Leu Arg Asp Leu Ile Glu Thr Thr Ile Val Glu Tyr Glu Tyr Asp
260 265 270
Lys Ala Val Ala Cys Ala Thr Pro Ala Tyr Ala Trp Ile Val Val Pro
275 280 285
Pro Ile Gly Leu Ile Ala Ala Ile Ile Val Ala Gly Ile Tyr Gly Asp
290 295 300
Lys Ala Val Lys Ala Arg Asn Arg Leu Glu Glu Leu Arg Lys Asp Leu
305 310 315 320
Ala Thr Ala Gln Gly Asn Glu Lys Leu Ser Leu Met Leu Thr Cys Ser
325 330 335
Leu Gly Val Ala Arg Asp Ser Leu Ser Phe Ile Gln Arg Asp Ile Gln
340 345 350
Ala Ala Leu Pro Val Ile Gln Lys Ile Gln Gly Val Trp His Ala Ile
355 360 365
His Thr Gly Leu Asp Asp Leu Cys Glu Ile Ile His Glu Asn Ile Asp
370 375 380
Glu Ala Ile Ile Glu Ile Lys Lys Met Gly Ile Ala Ile Ala Leu Glu
385 390 395 400
Gln Trp Ser Ser Leu Ala Val Lys Ala Glu Ala Tyr Gln Ala Asn Ala
405 410 415
Tyr Ile Glu Gln Pro Lys Thr Thr Thr Glu Gly
420 425
<210> 115
<211> 289
<212> БЕЛОК
<213> Bacillus sp.
<400> 115
Met Asn Pro Leu Val Phe Pro Ile Arg Gln Leu Glu Asp Asn Asn Ser
1 5 10 15
Pro Phe Ile Leu Ser Val Asp Glu Trp Arg Arg Phe Ser Asp Tyr Met
20 25 30
Lys Lys Cys Asn Tyr Leu Pro Ile Asn Ile Asp Ala Leu Glu Glu Tyr
35 40 45
Ile Cys Thr Lys Ser Asp Gly Lys Phe Lys Glu Ser Trp Asp Arg Leu
50 55 60
Val Lys Glu Asp Thr Pro Phe Cys Ile Met Leu Phe Ile Ser Trp Gly
65 70 75 80
Val Thr Asn Asn Ile Lys Gln Ala Ser Leu Phe Trp Gly Asn Gly Glu
85 90 95
Pro Ala Glu Ile Ile Ser Leu Ala Gln Asn Ile Val Thr Asp Thr Asp
100 105 110
Leu Ile Cys Ile Lys His His Asp Asn Phe Asn Gln Ile Leu Leu Glu
115 120 125
Ala Gln Asn Gly Asn Leu Ser Pro Asn Thr Lys Ser Lys Phe Leu Tyr
130 135 140
Ile Cys Asn Asp Leu Ser Asn His Thr Leu Lys Tyr His Gln Gln Ala
145 150 155 160
Gln Lys Met Thr Arg Asp Leu Ser Gln Phe Tyr Thr Thr Ile Gly Glu
165 170 175
Cys Asp Tyr Ala Tyr Glu Lys Cys Thr Glu Met Ile Pro Arg Ile Gly
180 185 190
Ile Arg Asn His Leu Glu Tyr Ser Val Val Tyr Gly Thr Phe Thr Ser
195 200 205
Leu Ala Ser Gln Leu Gly Arg Val Ile Pro Ser Glu Thr His Ile Leu
210 215 220
Pro Ile Ile Arg Lys Ile His Arg Ile Trp Ser Ala Ile Ser Tyr Asp
225 230 235 240
Leu Lys Glu Leu Ser Arg Asp Met Ser Glu Asp Leu Asp Asn Leu Pro
245 250 255
Ala Ile Ile Ala Ala Leu Glu Leu Glu Leu Ala Phe Glu Asn Leu Ser
260 265 270
Ala Ile Arg Lys Glu Val Glu Tyr Phe Tyr Lys Asn Tyr Glu Thr Ser
275 280 285
Pro
<210> 116
<211> 363
<212> БЕЛОК
<213> Bacillus sp.
<400> 116
Leu Lys Ser Asn Thr Lys Leu Ala Trp Asp Val Ser Gly Asn Ser Ile
1 5 10 15
Asp Asn Asn Arg Ala Ile Leu Leu Trp Asp Thr Ala Ser Arg Gly Asp
20 25 30
Asn Glu Gln Phe Val Phe Phe Pro Leu Asp Gly Gln Asn Tyr Ala Ile
35 40 45
Val Asn Lys Asn Ser Gly Lys Pro Val Thr Phe Gly Gly Ser Thr Pro
50 55 60
Ala Leu Ile Thr Gly Gly Gly Leu Pro Asn Met Glu Ala Leu Gln Gln
65 70 75 80
Arg Ser Trp Thr Gly Ser Pro Thr Glu Gln Trp Tyr Leu Arg Asp Lys
85 90 95
Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly Asn Gln Lys Val Ala
100 105 110
Ser Phe Ala Tyr Thr Trp Ala Ser Gln His Val Asp Tyr Val Asp Leu
115 120 125
Asp Glu Ser Asn Pro Ser Asp Ser Asp Arg Val Phe Asn Ile Ala Lys
130 135 140
Asn Gly Phe Gly Gly Ile Gly Val Ser Ile Pro Gly Phe Asp Val Asp
145 150 155 160
Tyr Gly Thr Phe Ser Leu Pro Asn Leu Pro Ala Thr Gly Thr Arg Pro
165 170 175
Thr Val Pro Asp Tyr Asp Gly Val Ser Gly Pro Asn Gln Thr Leu Pro
180 185 190
Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Val Pro Cys Ile
195 200 205
Met Val Asn Asp Ser Gln Thr Ser Asp Tyr Thr Lys Ile His Asn Ser
210 215 220
Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Ala Ala Ser
225 230 235 240
Thr Val Leu Glu Pro Gly Ala Thr Gln Ser Ile Ser Phe Lys Thr Gly
245 250 255
Met Thr Ser Ser Asp Gln Gln Lys Met Thr Glu Thr Leu Ser Met Lys
260 265 270
Val Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Lys Thr Ala Ala Leu
275 280 285
Lys Ala Glu Ile Ser Lys Thr Leu Gln Thr Glu Thr Ser Thr Thr Asn
290 295 300
Thr Glu Leu Ser Glu Asp Thr Val Thr Thr Asn Phe Lys Asp Glu Val
305 310 315 320
Gly Lys Arg Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys Tyr Thr
325 330 335
Leu Lys Arg Ala Asp Gly Ser Glu Val Ser Gly Ala Trp Thr Val Lys
340 345 350
Asn Asn Lys Ile Thr Glu Ile Arg Lys Ser Lys
355 360
<210> 117
<211> 373
<212> БЕЛОК
<213> Bacillus sp.
<400> 117
Val Gly Lys Ser Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Ala Gly Asn Gln Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Phe Leu Ala Asp Ala Pro Ser
35 40 45
Glu Arg Phe Phe Val His Pro Leu Asp Asn Asn Tyr Phe Ala Met Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Val Ala Gly Asn Gln Thr Gly
65 70 75 80
Asn Asn Val Asn Ile Gln Gln Phe Glu Trp Leu Gly Asp Ala Pro Ser
85 90 95
Glu Tyr Trp Tyr Phe His Arg Glu Ala Asp Gly His Tyr Val Ile Glu
100 105 110
Ser Lys Leu Ser Gly Lys Val Leu Asp Ile Ala Gly Asn Gln Thr Gly
115 120 125
Asn Asn Ala Asn Val Gln Gln Tyr Glu Phe Leu Pro Asp Ala Pro Ser
130 135 140
Glu Arg Phe Ala Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Asn Thr Gln Pro Leu Ser Ser Val Pro Glu Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Glu Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val
180 185 190
Pro Ser Ile Ser Val Lys Asp Pro His Tyr Gly Ser Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Ser Glu Lys Tyr Asp Phe
225 230 235 240
Val Thr Lys Thr Gly Ile Lys Val Thr Asp Gln Glu Thr Ala Thr Arg
245 250 255
Thr Val Ser Trp Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly
260 265 270
Phe Ser Leu Gly Met Ser Ser Gln Tyr Ser Gln Glu Leu Gln Thr Ser
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Thr Gln Glu His His
290 295 300
Ile Ile Asn Pro Phe Ser Glu Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Tyr Val Gln Arg Lys Asn Gly Thr Ile Val Asn Ser
325 330 335
Pro Trp Ala Met Thr Asp Lys Thr Lys Ala His Ala Val Thr Phe Pro
340 345 350
Lys Ser Thr Gly Asn Val Leu Asn Gly Ser Thr Lys Gln Gln Ser Lys
355 360 365
Ser Gly Ser Val Asn
370
<210> 118
<211> 595
<212> БЕЛОК
<213> Bacillus sp.
<400> 118
Met Lys Tyr Asn Asp Arg Lys Asp Ala Lys Arg Lys Tyr Lys Gln Ala
1 5 10 15
Leu Leu Ala Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Ser Thr Ala Ser Ala Phe Ala Glu Glu Lys Lys Thr Glu Val Gln Pro
35 40 45
Gln Gln Ala Thr Pro Asn Ser Asn Ser Leu Gly Ala Gly Gly Gly Tyr
50 55 60
Glu Ser Asp Ala Leu Lys Ala Leu Thr Asp Lys Ile Asn Met Asp Tyr
65 70 75 80
Leu Lys Lys Ala Gly Ser Val Ala Ala Asp Thr Leu Lys Ala Ala Tyr
85 90 95
Lys Asp Ala His Thr Asn Asn Gly Asn Tyr Asn Asn Thr Phe Arg Thr
100 105 110
Leu Thr Met Thr Thr Ser Ala Leu Ile Pro Tyr Gly Gly Val Leu Ile
115 120 125
Ser Pro Ile Ile Gly Leu Leu Trp Pro Glu Asp Leu Thr Ala Glu Asn
130 135 140
Asn Lys Met Ala Ala Leu Ile Lys Gln Leu Asp Glu Met Met Asp Thr
145 150 155 160
Lys Val Thr Asp His Val Val Arg Arg Leu Lys Thr Glu Val Asn Asp
165 170 175
Val Lys Lys Gln Leu Glu Leu Phe Glu Gln Asn Val Asn Thr Gly Lys
180 185 190
His Lys Ser Gly Ser Gly Leu Tyr Ser Leu Thr Glu Glu Asp Gly Asn
195 200 205
Ala Ile Asp Ala Lys Asp Ile His Lys Ala Phe Asn Ser Leu Leu Glu
210 215 220
His Ser Lys Glu Glu Met Gln Glu Val Ala Ala Leu Pro Ile Tyr Thr
225 230 235 240
Ala Met Ala Thr Ala His Leu Glu Phe Leu His Phe Leu Glu Gln Asn
245 250 255
Gly Thr Lys Ser Ile Ile Gly Phe Thr Ser Asn Ala Leu Asn Asn Ile
260 265 270
Leu Lys Thr Arg Glu Gly Leu Glu Asn Glu Thr Phe Gly Gln Lys Ile
275 280 285
Lys Asp Leu Ala Asp Lys Tyr Thr Lys His Val Gln Glu Thr Tyr Asn
290 295 300
Ile Gly Arg Asp Lys Tyr Met Ala Lys Met Gln Ala Ile Thr Thr His
305 310 315 320
Ser Met Asn Glu Asn Asp Glu Tyr Ala Asn Leu Ala Tyr Leu Lys Ser
325 330 335
Leu Asp Asp Ala Arg Ser Glu Met Gly Tyr Ser Gln Ser Lys Ile Asp
340 345 350
Lys Ile Lys Asn Asp Ile Lys Asn Tyr Gln Asn Leu Ile Lys Glu Lys
355 360 365
Ala Asp Phe Arg Asp Gln Thr Ala Glu Asn Ala Ala Phe Lys Leu Thr
370 375 380
Ala Met Gly Lys Trp Asn Gln Gln Gly Asp Lys Trp Tyr Tyr Ile Asn
385 390 395 400
Asn Gln Gly Glu Asn Gln Thr Gly Trp Leu Gln Asp Gly Ser Lys Tyr
405 410 415
Tyr Tyr Phe Ser Pro Glu Lys Thr Asp Lys Phe Glu Lys Gly Gln Met
420 425 430
Val Thr Gly Lys Val Thr Thr Asp Lys Gly Glu Thr Tyr Tyr Phe Asn
435 440 445
Pro His Gly Gly Gln Met Gln Thr Gly Trp Ile Gln Asp Asp Gly Lys
450 455 460
Gln Tyr Tyr Phe Ser Pro Ala Asn Asn Thr Lys Asn Tyr Asp Asn Glu
465 470 475 480
Ser Phe Lys Glu Gly Gln Met Met Thr Gly Trp Leu Ala His Leu Asp
485 490 495
His Tyr Tyr Tyr Met Ser Pro Gly Asp Asp Lys Thr Asn Pro Tyr Gln
500 505 510
Lys Gly Lys Thr Phe Lys Lys Gly Glu Met Trp Thr Gly Trp Leu Glu
515 520 525
Asn Pro Lys Lys Ala Asn Glu Trp Tyr Tyr Met Thr Pro Val Ala Arg
530 535 540
Asp Tyr Lys Val Pro His Leu Ile Gly Asn Asp Asp His Ile Ile Arg
545 550 555 560
Pro Val Gly Ser Met Leu His Asp Glu Tyr Leu Glu Lys Gln Asp Ser
565 570 575
Gly Asp Gly Trp Arg Tyr Tyr Phe Asn Glu Ser Gly Ile Asn Thr His
580 585 590
Arg Tyr Asn
595
<210> 119
<211> 380
<212> БЕЛОК
<213> Bacillus sp.
<400> 119
Met Lys Lys Met Leu Ile Thr Gly Leu Leu Ile Thr Gly Val Ser Thr
1 5 10 15
Ser Tyr Phe Ala Pro Leu Asn Thr Leu Ala Asp Thr Pro Pro Asn Ser
20 25 30
Gln His Thr Asn Ser Pro Asn Glu Phe Asn Val Asn Arg Leu Lys Asp
35 40 45
Ser Met Val Ala Leu Gly Ala Arg Met Pro Val Ile Glu Gly Tyr Gly
50 55 60
Leu Val Leu Leu Lys Gln Pro Asn Ile Glu Ala Pro Ala Ile His Gly
65 70 75 80
Ile Ala Val Asp Gln Gln Thr Ala Arg Lys Tyr Val Gln Asp Trp Leu
85 90 95
Asp Ile His Asn Pro Lys Leu Phe Ser Val Asn Gln Asp Met Met Arg
100 105 110
Phe Gly Lys Lys Phe Thr Thr Tyr Tyr Asp Lys Leu Leu Glu Leu Ala
115 120 125
Gly Gln Met Asn Glu Asn Pro Gln Ala Lys Val Ala Phe Val Lys Ala
130 135 140
Phe Asn Gly Ile Gln Asn Gln Met Gln Ala Thr Phe Leu Ala Met Gln
145 150 155 160
Asp Val Ser Ala Asp Leu Ser Asn Tyr Lys Asn Glu Leu Asp Lys Asp
165 170 175
Ser Lys Gln Phe Ser Thr Lys Val Gly Arg Ala Ile Glu Leu Tyr Lys
180 185 190
Gly Ser Asn Gly Asp Ile Glu Lys Leu Arg Thr Gln Ile Glu Lys Tyr
195 200 205
Asp Asp Glu Ile Gln Asn Glu Ile Thr Lys Leu Leu Ser Leu Pro Ser
210 215 220
Glu Asn Leu Lys Asp Leu Ile Asp Ile Gly Glu Gln Ile Ala Gln Ile
225 230 235 240
His Ile Asp Ala Leu Asn Lys Ser Ile Asn Leu Ser Asp Val Gly Leu
245 250 255
Leu Phe Thr Asn Ser Gly Lys Ala Leu Asn Thr Asp Val Gln Glu Leu
260 265 270
Asn Asn Lys Ile Thr Gln Lys Arg Gln Gln Lys Met Glu Ser Ile Lys
275 280 285
Gln Leu Ala Gly Ile Glu Ile Gln Ala Thr Gln Met Ile Leu Val Asp
290 295 300
Gln Lys Leu Asn Asn Phe Thr Lys Leu Ile Lys Gln Gln Ser Gln Ser
305 310 315 320
Phe Asp Lys Val Val Ser Ser Trp Glu Arg Phe Asn Thr Thr Val Met
325 330 335
Gln Val Gln Ala Ser Leu Asn Ser Asp Lys Lys Ile Asp Ser Asn Ala
340 345 350
Leu Gln Thr Gln Leu Asn Glu Leu Lys Lys Phe Thr Asp Thr Leu Asn
355 360 365
Ile Gln Thr Gln Glu Tyr Glu Gly Lys Leu Gln Lys
370 375 380
<210> 120
<211> 298
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 120
Met Lys Lys Met Leu Ile Thr Gly Leu Leu Ile Thr Gly Val Ser Thr
1 5 10 15
Ser Tyr Phe Ala Pro Leu Asn Thr Leu Ala Asp Thr Pro Pro Asn Ser
20 25 30
Gln His Thr Asn Ser Pro Asn Glu Phe Asn Val Asn Arg Leu Lys Asp
35 40 45
Ser Met Val Ala Leu Gly Ala Arg Met Pro Val Ile Glu Gly Tyr Gly
50 55 60
Leu Val Leu Leu Lys Gln Pro Asn Ile Glu Ala Pro Ala Ile His Gly
65 70 75 80
Ile Ala Val Asp Gln Gln Thr Ala Arg Lys Tyr Val Gln Asp Trp Leu
85 90 95
Asp Ile His Asn Pro Lys Leu Phe Ser Val Asn Gln Asp Met Met Arg
100 105 110
Phe Gly Lys Lys Phe Thr Thr Tyr Tyr Asp Lys Leu Leu Glu Leu Ala
115 120 125
Gly Gln Met Asn Glu Asn Pro Gln Ala Lys Val Ala Phe Val Lys Ala
130 135 140
Phe Asn Gly Ile Gln Asn Gln Met Gln Ala Thr Phe Leu Ala Met Gln
145 150 155 160
Asp Val Ser Ala Asp Leu Ser Asn Tyr Lys Asn Glu Leu Asp Lys Asp
165 170 175
Ser Lys Gln Phe Ser Thr Lys Val Gly Arg Ala Ile Glu Leu Tyr Lys
180 185 190
Gly Ser Asn Gly Asp Ile Glu Lys Leu Arg Thr Gln Ile Glu Lys Tyr
195 200 205
Asp Asp Glu Ile Gln Asn Glu Ile Thr Lys Leu Leu Ser Leu Pro Ser
210 215 220
Glu Asn Leu Lys Asp Leu Ile Asp Ile Gly Glu Gln Ile Ala Gln Ile
225 230 235 240
His Ile Asp Ala Leu Asn Lys Ser Ile Asn Leu Ser Asp Val Gly Leu
245 250 255
Leu Phe Thr Asn Ser Gly Lys Ala Leu Asn Thr Asp Val Gln Glu Leu
260 265 270
Asn Asn Lys Ile Thr Gln Lys Arg Gln Gln Lys Met Glu Ser Ile Lys
275 280 285
Gln Leu Ala Gly Ile Glu Ile Gln Ala Thr
290 295
<210> 121
<211> 373
<212> БЕЛОК
<213> Bacillus sp.
<400> 121
Val Gly Lys Ser Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Ser Gly Asn Glu Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Lys Asp Ala Pro Ser
35 40 45
Glu Arg Phe Phe Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Ile Ser Gly Asn Glu Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Phe Glu Trp Leu Gly Asp Ala Pro Ser
85 90 95
Glu Tyr Trp Tyr Phe His Arg Glu Ala Asp Gly Tyr Tyr Val Ile Glu
100 105 110
Ser Lys His Ser Gly Lys Val Leu Asp Ile Ser Gly Asn Glu Thr Gly
115 120 125
Asn Asn Ala Asn Val Gln Gln Phe Glu Phe Leu Ala Asp Ala Pro Ser
130 135 140
Glu Arg Phe Ala Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Asn Thr Gln Pro Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Glu Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val
180 185 190
Pro Ala Ile Ser Val Lys Asp Pro His Tyr Gly Glu Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr
225 230 235 240
Thr Ser Lys Thr Gly Ile Lys Val Thr Asp Gln Glu Ala Ala Thr Lys
245 250 255
Thr Val Ser Trp Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly
260 265 270
Phe Ser Val Gly Met Ser Ser Gln Tyr Ser Thr Gln Leu Gln Ala Ser
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu
290 295 300
Ile Lys Asn Pro Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Ser Val Gln Arg Lys Asn Gly Thr Leu Val Asn Ser
325 330 335
Pro Trp Thr Met Thr Asp Lys Thr Arg Thr His Ala Val Thr Tyr Pro
340 345 350
Asn Ala Glu Glu Lys Ala Leu Asn Glu Asn Ala Lys Gln Leu Ser Lys
355 360 365
Val Glu Ser Val Asn
370
<210> 122
<211> 497
<212> БЕЛОК
<213> Bacillus sp.
<400> 122
Met Lys Tyr Lys Asp Arg Ile Gln Ala Lys Arg Asn Tyr Lys Gln Ala
1 5 10 15
Ile Phe Ala Thr Ala Thr Thr Leu Thr Leu Gly Val Ser Ala Leu Ala
20 25 30
Gly Pro Ala Ser Ala Phe Ala Ala Glu Gln Lys Thr Ala Asp Asn Ile
35 40 45
Lys Ala Val Gln Thr Gln Ser Gln Ile Lys Ala Met Thr Asp Leu Asp
50 55 60
Thr Asn Asn Phe Asp Lys Phe Ile Asn Gly Gln His Phe Ser Lys Asp
65 70 75 80
Ser Asp Val Gly Lys Phe Leu Ile Lys Leu Lys Thr Ile Gly Ile Asn
85 90 95
Ser Leu Thr Lys Ser Ala Asn Asn Gly Thr Ile Asp Phe Asn Asn Phe
100 105 110
Ala Arg Asp Leu Val Ile Ser Gly Ala Gly Leu Ile Pro Phe Gly Gly
115 120 125
Ala Ile Leu Ser Pro Phe Ile Ala Met Leu Trp Pro Gln Ser Ser Gln
130 135 140
Ser Gln Met Asp Gln Leu Val Lys Lys Phe Ser Ala Met Met Asp Glu
145 150 155 160
Lys Ile Val Asp Tyr Asp Asn Gly Ala Leu Lys Gln Gln Ala Ile Ala
165 170 175
Leu Gln Asp Arg Leu Ala Ala Phe Glu Asn Leu Val Asn Arg Ile Glu
180 185 190
Gln Lys Thr Ser Thr Phe Ile Thr Asp Gln Asp Val Ala Ala Gln Gln
195 200 205
Ala Leu Ala Leu Asn Asp Ala Phe Asn Asp Leu Leu Arg Ser Cys Gln
210 215 220
Lys Asp Glu Gln Lys Lys Ser Glu Leu Pro Leu Tyr Thr Gln Ile Ala
225 230 235 240
Thr Ala His Leu Val Phe Leu Lys Tyr Val Val Glu Asn Gly Gly Lys
245 250 255
Gly Thr Ile Leu Asn Phe Gln Gln Asn Gly Leu Gln Leu Phe Lys Asp
260 265 270
Ala Gln Glu Lys Ala Ile Lys Glu Tyr Pro Gln Tyr Ile Ala Gln Val
275 280 285
Ala Tyr Gln Ala Leu Ala Gln Ile Ser Thr Lys Glu Leu Gly Phe Ser
290 295 300
Ala Val Asp Asn Glu Glu Tyr Thr His Gln Ile Tyr Ile Gly Ala Lys
305 310 315 320
Lys Thr Tyr Asp Ser Leu Gln Arg Pro Leu Leu Pro Lys Glu Lys Glu
325 330 335
Gln Leu Glu Gln Ala Ile Arg Met Met Asn Lys Ile Glu Gly Gly Tyr
340 345 350
Lys Leu Ser Leu Pro Thr Asn Ala Ala Asn Ala Val Tyr Ala Tyr Arg
355 360 365
Ala Asn Thr Val Asp Asn Leu Ala Phe Asn Thr Ile Leu Asn Glu Ser
370 375 380
Thr Gly Asn Lys Lys Thr Gly Trp Val Arg Glu Asn Asn Ile Tyr Phe
385 390 395 400
Tyr Glu Lys Asp Gly Ile Arg Gln Asn Ser Gly Trp Phe Lys Asp Gly
405 410 415
Asp Ala Trp Tyr Tyr Leu Asp His Lys Asp Gly His Met Ala Thr Gly
420 425 430
Trp Val Lys Asp Asn Asp Lys Trp Tyr Phe Leu Ser Ala Phe Asp Asp
435 440 445
Pro Lys Tyr Asn Phe Lys His Gly Gln Met Tyr Phe Gln Pro Lys Glu
450 455 460
Thr Gly Gly Arg Lys Asn Ala Ser Ile Leu Ile Asp Asp Gly Tyr Gly
465 470 475 480
Lys His Tyr Asn Phe Asp Glu Asn Gly Val Cys Leu Asn Pro Asn Gly
485 490 495
Glu
<210> 123
<211> 393
<212> БЕЛОК
<213> Pseudomonas sp.
<400> 123
Met Gln Gln Glu Glu Thr Thr Ser Ala Ala Val Tyr Thr Leu Asp Glu
1 5 10 15
Met Leu Met Ala Ser Gly Asn Phe Met Ser Val Ala Ser Ala Gln Asn
20 25 30
Ala Glu Val Ala Arg Asp Pro Gly Leu Ile Leu Thr Thr Glu Asp Ala
35 40 45
Arg Gln Ile Arg Ser Tyr Val Arg Thr Gly Leu Glu Leu Pro Thr Asp
50 55 60
Leu Glu Gln Val Arg Gln Leu Leu Gly Asn Tyr Ser Ser Asp Ile Lys
65 70 75 80
Gly Leu Ala Pro Gln Asp Ile Arg Asp Leu Tyr Ala Met Ile Asn Ala
85 90 95
His Cys Gln Thr Trp Ala Pro Leu Glu Ser Asp Ile Arg Lys Val Gly
100 105 110
Ser Asp Leu Tyr Ala Phe Ser Asp Ala Leu Leu Glu Thr Ala Arg Thr
115 120 125
Leu Val Glu Phe Val Thr Gly Leu Glu Ser Tyr Gln Ser Leu Lys Val
130 135 140
Ser Asp Leu Ser Pro Glu Gln Ile Asp Ala Leu Pro Pro Val Ala Leu
145 150 155 160
Ala Pro Gly Asp Thr Leu Lys Ile His Ile Leu Ala Gly Phe Phe Asp
165 170 175
Glu Leu Asn Thr Leu Leu Glu Glu His Arg Val Ser Thr Thr Arg Val
180 185 190
Lys Ala Gly Val Gly Glu Phe Arg Lys Thr Leu Arg Gly Ser Ile Ala
195 200 205
Pro Asn Val Ser Leu Lys Val Arg Leu Ser Asn Ser Ala Asp Thr Ser
210 215 220
Arg Glu Val Ala Arg Leu Asn Gly Glu Leu Ala Ser Leu Thr Gln Arg
225 230 235 240
Ile Asp Gln Lys Thr Ala Glu Tyr Gly Glu Tyr Val Lys Tyr Ala Leu
245 250 255
Ile Gly Val Trp Trp Gly Pro Ile Gly Ala Ala Ile Thr Gly Ser Ile
260 265 270
Tyr Gly Ile Lys Ala Glu Asn Val Arg Lys Glu Lys Asn Glu Leu Ile
275 280 285
Glu Ser Lys Arg Arg Val Glu Gln Gln Leu Met Lys Leu Asn Thr Leu
290 295 300
Leu Gly Gly Leu Arg Thr Leu Glu Thr Asn Leu Glu Asp Leu Glu Gly
305 310 315 320
Arg Ile Asp Gly Ala Thr Val Gly Ile Ala Gly Leu Glu Ser Ile Trp
325 330 335
Val Leu Leu Glu Glu Leu Val Lys Ser Ser His Lys Arg Val Ala Asp
340 345 350
Leu Asn Asn Ala Thr Tyr Leu Ala Ile Leu Val Leu Arg Phe Asn Ile
355 360 365
Val Ile Ser Asn Trp Leu Asp Ile Lys Lys Gln Ser Phe Asp Leu Leu
370 375 380
Thr Ala Phe Asp Asn Ala Ile Asp Arg
385 390
<210> 124
<211> 381
<212> БЕЛОК
<213> Ensifer sp.
<400> 124
Met Ala Ile Tyr Leu Leu Asp Met Lys Ala Asn Gly Thr Ala Pro Gly
1 5 10 15
Gln Thr Leu Leu Tyr Thr Arg Phe Gly Cys Asn Cys Ala Val Ser Lys
20 25 30
Gln Gly Glu Asp Ala Met Asn Glu Val Leu Arg Asn Ser Val Gly Gln
35 40 45
His Ser His Val Ser Lys Arg Ala Leu Arg Ala Arg Arg Ser Pro Val
50 55 60
Val Phe Ile Asn Thr Thr Gln Glu Glu Ala Glu Gln Phe Gln Glu Ile
65 70 75 80
Val Ala Ile Asp Asp Ile Asp Tyr Leu Thr Gln Ala Ile Ala Leu Thr
85 90 95
Ala Leu Phe Asn Gly Ala Ile Asp Asn Thr Thr Gly Arg Phe Ile Pro
100 105 110
Gly Lys Ala Arg Glu Leu Ile Ala Asn Tyr Asn Glu Ser Leu Asp Val
115 120 125
Asn Ser Gln Pro Tyr Lys Ile Gly Ile Phe Asn Thr His Gln Thr Thr
130 135 140
Leu Thr Gln Gln Asn Ser Ala Val Ser Ala Met Ile Gly Gln Ile Leu
145 150 155 160
Glu Thr Leu Lys Arg Val Met Gly Val Ala Leu Gly Ala Ser Ala Val
165 170 175
Thr Gln Met Thr Ala Ala Val Thr Asp Ala Phe Thr Asn Leu Asp Glu
180 185 190
Gln Ser Gly Asp Ala Trp Ile Phe Trp Glu Lys Lys Thr Ser Asn Lys
195 200 205
Thr Thr Tyr Ser Tyr Ala Ile Leu Phe Ala Phe Gln Asp Gly Ser Thr
210 215 220
Gly Lys Leu Met Phe Ala Leu Pro Met Ser Leu Glu Ile Glu Val Asp
225 230 235 240
Val Ser Tyr Glu Arg Val Leu Phe Ile Thr Val Glu Asp Lys Glu Thr
245 250 255
Tyr Ser Val Lys Leu Asp Thr Met Lys Val Gly Gln Leu Leu Phe Pro
260 265 270
Lys Ser Pro Gly Ala Asn Ala Leu Gln Ser Ala Arg Arg Leu Gly Thr
275 280 285
Arg Ser Gly Ser Ala Asp Leu Leu Ala Asp Pro Arg Ala Ile Thr Asp
290 295 300
Val Val Val Thr Asn Trp Ala Lys Thr Thr Thr Phe Ala Thr Ala Ala
305 310 315 320
Thr Gly Phe Tyr Thr Asn Asp His Pro Leu Ala Gln Val Met Ala Ser
325 330 335
Glu Pro Asn Ile Val Asn Pro Leu Tyr Glu Gly Asn Gln Tyr Leu Val
340 345 350
Ser Phe Thr Leu Asp Gly Ala Arg Gln Thr Leu Gly Phe Phe Leu Asn
355 360 365
Cys Thr Leu Pro Asp Gly Glu Leu Trp Phe Val Ser Ile
370 375 380
<210> 125
<211> 295
<212> БЕЛОК
<213> Bacillus sp.
<400> 125
Met Gln Arg Gly Ile Tyr Met Asn Tyr Val Thr Phe Ser Thr Arg Glu
1 5 10 15
Leu Gly Gly Lys Ser Ser Pro Phe Ile Leu Ser Val Ala Glu Trp Lys
20 25 30
Ala Phe Ser Asp Tyr Met Lys Gln Cys Val Ser Leu Pro Thr Ser Val
35 40 45
Asp Ile Leu Gln Glu Arg Ile Cys Asn Ile Asn Glu Gly Gln Leu Arg
50 55 60
Thr Ser Trp His Arg Leu Leu Ala Glu Asp Ile Thr Tyr Pro Arg Met
65 70 75 80
Val Thr Thr Gly Leu Glu Ile Thr Thr Lys Val Lys Gln Ser Ser Arg
85 90 95
Phe Trp Asp Ala Gly Gly Arg Ala Gly Ile Ile Ser Leu Ala Gln Asn
100 105 110
Ile Val Ala Tyr Ala Asp Leu Ile Cys Ile Lys His His Asp Asp Leu
115 120 125
Asn Gln Val Leu Leu Glu Ala Gln Asn Gly Asn Leu Ser Pro Asn Ser
130 135 140
Lys Ser Lys Phe Ile Asn Ile Cys Lys Asp Leu Ser Glu His Ala Leu
145 150 155 160
Arg Tyr His Gln Gln Ala Gln Thr Met Met Thr Tyr Leu Ser Glu Phe
165 170 175
Tyr Thr Glu Ile Glu Gly Cys Lys Asp Ala His Gln Asn Cys Thr Asp
180 185 190
Met Ile Ser Arg Ile Gly Met Arg Asn His Leu Glu Tyr Ala Val Val
195 200 205
His Asn Thr Val Thr Phe Leu Leu Gly Ala Ile Arg Arg Ile Ile Pro
210 215 220
Ser Glu Thr Lys Ile Leu Pro Val Ile Arg Lys Ile His Leu Ile Leu
225 230 235 240
Gly Ala Ile Ser Tyr Asp Leu Lys Glu Leu Gly Asp Asp Ile Gly Gln
245 250 255
Asp Leu Asp Asn Leu Pro Ala Ile Ile Ala Ala Leu Glu Leu Glu Leu
260 265 270
Ala Phe Glu Asn Trp Ser Ala Ile Arg Gln Glu Ala Glu Asp Phe His
275 280 285
Lys Asn Ala Glu Asn Phe Ser
290 295
<210> 126
<211> 554
<212> БЕЛОК
<213> Bacillus sp.
<400> 126
Met Asn Tyr Thr Asn Arg Lys Asn Ala Lys Arg Lys Tyr Lys Lys Ala
1 5 10 15
Ile Leu Ala Thr Ile Thr Thr Met Thr Leu Gly Val Ser Thr Phe Gly
20 25 30
Gly Thr Ala Ser Ala Phe Ala Ala Val Lys Ala Ser Asp Ile Ser Gln
35 40 45
Gly Leu Gln Pro Leu Thr Asn Ala Leu Lys Ile Lys Val Thr Asn Pro
50 55 60
Glu Ala Leu Ala Ala Gly Leu Ala Trp Thr Thr Gly Gln Ala Thr His
65 70 75 80
Phe Leu Glu Lys Ser Glu Met Gly Gly Met Ile Asn Trp Asn Asn Ala
85 90 95
Val Lys Asp Leu Thr Ile Ala Ser Ile Gly Leu Ile Pro Ile Gly Gly
100 105 110
Ala Ile Leu Ser Pro Ile Leu Ser Leu Leu Trp Pro Thr Glu Thr Ser
115 120 125
Asn Asp Gln Met Gln Lys Met Leu Asn Glu Val Ser Thr Met Ile Glu
130 135 140
Glu Asn Ile Ala Asp Tyr Asp Leu Asp Thr Leu Lys Pro Lys Val Asp
145 150 155 160
Thr Leu Lys Asp Arg Ile Ala Thr Phe Glu Lys Ala Ile Asn Lys Arg
165 170 175
Leu Ala Gly Leu Pro Thr Gly Gly Leu Leu Asn Ile Glu Gln Val Asn
180 185 190
Ala Gln Asp Ala Arg Glu Ile Asn Asn Lys Phe Lys Glu Val Leu Asn
195 200 205
Asp Cys Ser Lys Val Lys Ala Ser Thr Lys Glu Pro Tyr Thr Gly Glu
210 215 220
Leu Pro Ile Tyr Thr Thr Ile Ala Thr Gly His Leu Leu Phe Leu Lys
225 230 235 240
Phe Ile Lys Glu Asn Thr Asn Asn Pro Val Leu Gln Leu Asp Gln Lys
245 250 255
Ile Ile Glu Arg Asp Phe Gln Asp Asp Phe Asp Asn Ala Thr Glu Arg
260 265 270
Tyr Ser Lys His Ile Glu Asp Ile Tyr Ile Lys Gly Asp Gly Lys Ile
275 280 285
Val Lys Lys Met Lys Glu Ile Glu Asn Lys Thr Ala Glu Ala Gln Ser
290 295 300
Pro Asp Asp Lys Tyr Ala Asn Leu Gln Ala Leu Phe Ala Arg Leu Gln
305 310 315 320
Thr Ala Lys Thr Leu Leu Ser Asn Leu Thr Ala Pro Gly Thr Gln Pro
325 330 335
Gly Phe Gly Gln Gln Ile Arg Ile Asn Glu Leu Asn Ser Asp Ile Lys
340 345 350
Met Leu Glu Lys Asp Ile Pro Val Tyr Glu Lys Leu Ile Asp Lys Arg
355 360 365
Gln Asp Tyr Lys Asp Thr Thr Ile Asp Asn Glu Ala Phe Leu Leu Ala
370 375 380
Ser Gly Lys Thr Pro Ser Lys Thr Pro Ser Lys Thr Pro Ser Lys Thr
385 390 395 400
Pro Ser Val Lys Ile Ala Asp Gly Ser Tyr Lys Ile Thr Ser Met Gly
405 410 415
Gly Asn Leu Asn Leu Asp Tyr Ser Ser Tyr Ser Ser Ser Glu Pro Gly
420 425 430
Val Val Leu Trp Asn Pro Ser Glu Asp Lys Ser Gln His Trp Lys Leu
435 440 445
Glu Tyr Asp Glu Ser Lys Lys Ala Tyr Arg Ile Ile Ser Glu Arg Asp
450 455 460
Pro Asn Lys Cys Ile Ala Arg Asn Leu Trp Ser Gln Asn Leu Gly Val
465 470 475 480
Ile Asp His His Leu Asp Thr Val Phe Ile Thr Lys Lys Glu Asn Asn
485 490 495
Asp Glu His Phe Trp Thr Leu Glu Asp Ala Gly Asp Gly Tyr Phe Phe
500 505 510
Ile His Ser Phe Ala Asp Pro Gln Met Val Leu Asp Val Lys Gly Gly
515 520 525
Ala Phe Asn Met Gly Asp Thr Leu His Val Gln Pro Lys Ser Asn Tyr
530 535 540
Pro Asn Ala Glu Lys Phe Lys Phe Val Lys
545 550
<210> 127
<211> 382
<212> БЕЛОК
<213> Bacillus sp.
<400> 127
Met Met Lys Lys Thr Tyr Lys Leu Met Ala Leu Ser Thr Leu Val Thr
1 5 10 15
Thr Leu Ala Met Gly Ser Met Val Pro Ser Tyr Ala Ser Ala Ala Gly
20 25 30
Asn Thr Thr Asn Ala Thr Gln Ala Leu Ala Ser Lys Tyr Ser Asn Tyr
35 40 45
Gly Leu Gly Pro Glu Ala Leu Lys Lys Ala Ile Thr Glu Thr Lys Ser
50 55 60
Asn Met Leu Val Met Asp Leu Tyr Ala Leu Thr Leu Val Lys Gln Pro
65 70 75 80
Val Pro Asp Leu Asn Gly Leu Thr Ala Ile Ser Thr Thr Leu Lys Thr
85 90 95
Asn Val Lys Thr Asn Phe Glu Asn Ser Arg Lys Asp Ala Asp Thr Trp
100 105 110
Leu Asp Asp Leu Lys Pro Lys Ile Ile Ser Val Asn Gln Ser Ile Ile
115 120 125
Asn Tyr Asp Thr Lys Phe Gln Thr Tyr Cys Asn Thr Leu Ile Arg Ala
130 135 140
Ala Lys Glu Asn Asn Ile Pro Ile Leu Arg Ser Gly Leu Thr Arg Leu
145 150 155 160
Leu Gln Asp Ile Thr Lys Asn Lys Gly Asp Val Thr Gln Leu Leu Glu
165 170 175
Lys Leu Arg Asp Tyr Arg Ser Lys Leu Thr Thr Ser Thr Gln Asn Phe
180 185 190
Lys Asp Tyr Ser Asn Glu Val Thr Asn Ile Leu Thr Gly Gln Asp Ala
195 200 205
Gly Ile Pro Leu Met Glu Gln Gln Leu Ala Thr Asn Gln Glu Leu Ile
210 215 220
Asp Lys Asn Asn Lys Leu Leu Ala Ala Ser Ile Thr Gly Thr Ala Val
225 230 235 240
Gly Ser Trp Leu Thr Ala Thr Ile Val Leu Ala Pro Phe Gly Ala Pro
245 250 255
Ile Leu Gly Val Ser Ala Tyr Gly Val Asp Ser Leu Asn Lys Gln Ile
260 265 270
Ala Gln Ala Arg Asn Asn Ile Ile Glu Leu Thr Lys Asn Ile Ser Glu
275 280 285
Thr Arg Val Gln Ala Gly Lys Leu Gly Val Leu Lys Asn Thr Thr Asp
290 295 300
Glu Leu Thr Lys Thr Ile Asp Leu Ala Ile Asp Ala Leu Gln Asn Ile
305 310 315 320
Ser Asn Gln Trp Asn Thr Met Glu Ala Lys Tyr Asn Ser Val Leu Gln
325 330 335
Asn Leu Lys Thr Val Asp Pro Glu Phe Val Asp Phe Leu Glu Leu Asp
340 345 350
Leu Glu Val Ser Gln Thr Ser Trp Ala Asp Val Arg Lys His Ala Glu
355 360 365
Ala Leu Gln Asn Ser Glu Ile Lys Tyr Val Glu Asp Lys Lys
370 375 380
<210> 128
<211> 420
<212> БЕЛОК
<213> Bacillus sp.
<400> 128
Leu Lys Arg Gly Tyr Ala Met Lys Arg Gln Lys Ile Lys Lys Phe Pro
1 5 10 15
Leu Ser Lys Lys Val Ile Thr Gly Leu Phe Ala Gly Ala Met Ala Leu
20 25 30
Ser Ile Trp Ala Pro Ala Ser Gln Ala Ala Thr Pro Glu Asn Asn Arg
35 40 45
Tyr Tyr Ser Ile His Leu Lys Ser Asn Pro Lys Lys Val Trp Asp Val
50 55 60
Tyr Gln Asn Lys Thr Tyr Asp Asn Ala Gly Ile Leu Leu Tyr Asp Gly
65 70 75 80
Ser Asn Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu Asp Asp Gly
85 90 95
Lys Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val Gly Ile Gly
100 105 110
Asp Pro Ile Ala Asn Asp Asp Gly Val Arg Arg Met Thr Leu Phe Asn
115 120 125
Asn Glu Gly Leu Arg Gln Glu Ser Trp Thr Gly Ala Pro Asp Gln Gln
130 135 140
Trp Tyr Leu Arg Asn Gln Gly Tyr Asn Ser Tyr Glu Ile Val Asn Gln
145 150 155 160
Gly Tyr Gly Lys Val Ala Ser Tyr Ala Trp Gln Gly Thr Leu Ala Gln
165 170 175
Asn Trp Glu Tyr Ala Asp Leu Asp Glu Pro Asn Pro Ser Asp Ser Asp
180 185 190
Arg Val Phe Asn Ile Ser Asn Asn Pro Phe Gly Thr Glu Ile Asp Pro
195 200 205
Phe Glu Gly Thr Pro Ile Asn Met Lys Tyr Gly Thr Phe Ser Leu Pro
210 215 220
Glu Leu Pro Ala Thr Gly Thr Arg Pro Asp Val Pro Ala Tyr Asn Asp
225 230 235 240
Gly Ala Gly Pro Tyr Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val
245 250 255
Val Gly Ala Ser Leu Val Pro Cys Ile Met Val Asn Asp Ser Gln Ala
260 265 270
Ser Asp Tyr Thr Lys Met His Asn Ser Pro Tyr Tyr Thr Leu Val Lys
275 280 285
Glu Glu Tyr Trp Asp Lys Ala Ala Ser Thr Val Leu Thr Pro Gly Ala
290 295 300
Thr Gln Ser Ile Ser Phe Lys Thr Gly Met Thr Ser Thr Asp Gln Gln
305 310 315 320
Lys Met Thr Glu Thr Leu Ser Met Lys Val Gly Ala Asp Leu Gly Leu
325 330 335
Lys Phe Gly Asp Gln Thr Ala Ser Leu Lys Ala Glu Ile Ser Lys Thr
340 345 350
Leu Gln Thr Glu Ile Ser Thr Ser Asn Thr Glu Val Thr Glu Asp Thr
355 360 365
Val Thr Thr Ser Leu Thr Gly Glu Ala Gly Lys Thr Thr Gly Tyr Thr
370 375 380
Ile Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser
385 390 395 400
Ala Val Ser Gly Ser Trp Ile Val Lys Asn Asn Lys Glu Thr Val Leu
405 410 415
Arg Lys Thr Pro
420
<210> 129
<211> 423
<212> БЕЛОК
<213> Bacillus sp.
<400> 129
Leu Gly Lys Val Glu Val His Tyr Tyr Phe Phe Leu Leu Ser Asn Asn
1 5 10 15
Leu Ile Ile Arg Glu Leu Glu Gly Glu Val Leu Met Lys Lys Leu Pro
20 25 30
Ile Val Thr Gly Cys Ile Ile Thr Ala Ile Leu Thr Ser Gln Val Leu
35 40 45
Pro Leu Pro Ala Ser Ala Ala Asp Lys Lys Gln Val Asp Asn Gln Gly
50 55 60
Ile Asn Thr Ser Asn Val Val Val Ser Ser Thr Leu Gln Asn Leu Gly
65 70 75 80
Thr Gln Ser Val Leu Leu Lys Thr Tyr Ala Leu Thr Leu Leu Lys Gln
85 90 95
Pro Thr Val Ala Ile Glu Thr Met Pro Asn Leu Gly Val Tyr Gln Glu
100 105 110
Lys Ala Lys Gln His Ala Arg Tyr Trp Leu Asp Thr Val Gln Pro Ser
115 120 125
Phe Ile Ala Ala Asn Gln Gln Leu Ile Ser Phe Gln Gln Lys Phe Gln
130 135 140
Asn Tyr Tyr Asn Lys Leu Ile Glu Leu Ser Ser Lys Ile Glu Thr Asp
145 150 155 160
Asn Gln Ala Lys Gln Glu Phe Ile Ile Gly Ile Thr Lys Leu Gln Glu
165 170 175
Cys Leu Asn Asn Asp Gln Asn Glu Ile Gln Lys Met Ser Ser Gly Leu
180 185 190
Gln Lys Phe Gln Gln Asn Phe Asn Val Asp Ala Glu Asn Phe Ile Ser
195 200 205
Leu Ala Ala Thr Ala Lys Thr Ser Leu Ala Asn Lys Asn Gly Glu Val
210 215 220
Glu Gln Leu Val Lys Gln Ile Glu Thr Leu Asn Ser Asp Ile Thr Ser
225 230 235 240
Gln Ile Gly Val Ile Val Gly Gly Thr Ile Gly Met Val Ala Gly Ile
245 250 255
Gly Ala Ile Val Phe Gly Thr Ile Val Leu Phe Thr Ala Thr Val Ala
260 265 270
Thr Gly Gly Val Gly Ala Ala Val Val Val Pro Thr Leu Gly Ser Ile
275 280 285
Ala Pro Ala Leu Gly Gly Ile Val Gly Gly Thr Gly Gly Leu Ile Gly
290 295 300
Gly Gly Val Thr Val Gly Phe Ala Ala Lys Lys Leu Glu Glu Lys Arg
305 310 315 320
Lys Glu Leu Gln Glu Thr Thr Gln Lys Leu Glu Asn Ala Lys Ala Asp
325 330 335
Ala Ala Ala Leu Thr Leu Leu Val Gly Gln Val Asn Thr Phe Lys Asp
340 345 350
Thr Val Thr Lys Gly Lys Glu Ser Leu Glu Gly Phe Asp Asp Asn Trp
355 360 365
Tyr Glu Leu Lys Asn Asn Phe Asn Val Leu Gln Gln Asn Val Asn Lys
370 375 380
Ile Asn Pro Asp Ser Ser Val Leu Gln Asn Asn Leu Val Gln Ile Lys
385 390 395 400
Lys Lys Val Asp Gly Leu Ala Leu Gln Ala Lys Gln Gln Glu Lys Val
405 410 415
Ile Thr Asp Ile Ser Tyr Lys
420
<210> 130
<211> 386
<212> БЕЛОК
<213> Bacillus sp.
<400> 130
Val Lys Arg Asn Leu Ile Thr Gly Leu Leu Val Thr Ala Val Ser Thr
1 5 10 15
Ile Ser Leu Thr Ser Val Asn Val Tyr Ala Glu Glu Val Lys Phe Asn
20 25 30
Leu Lys Pro Thr Tyr Lys Gln Asn Glu Met Val Pro Asn Thr Val Ser
35 40 45
Asn Ser Ile Arg Met Leu Gly Ala Gln Ser Pro Leu Ala Gln Ala Tyr
50 55 60
Gly Leu Val Ile Leu Gln Gln Pro Asp Ile Lys Val Asn Ala Met Ser
65 70 75 80
Ser Leu Thr Asn His Gln Lys Phe Ala Lys Lys Asn Val Glu Glu Trp
85 90 95
Ile Asp Glu Tyr Asn Pro Lys Leu Ile Asn Leu Asn Gln Gln Met Met
100 105 110
Arg Tyr Ser Thr Arg Phe Asn Ser Tyr Tyr Ser Lys Leu Tyr Glu Leu
115 120 125
Ala Gly Lys Val Asn Glu Asp Ala Gln Ala Lys Glu Asp Phe Lys Asn
130 135 140
Ala Tyr Gly Lys Leu Leu Asp Gln Val Gln Thr Ile Gln Glu Asn Met
145 150 155 160
Asp Gln Thr Ser Leu Glu Leu Asn Arg Phe Lys Thr Gly Leu Asp Lys
165 170 175
Asp Ser Gln Ser Leu Ser Gln Lys Ala Asp Val Ala Ile Gln Ser Leu
180 185 190
Gln Gly Ser Asn Gly Asp Ile Thr Gln Leu Arg Ala Asp Ile Lys Arg
195 200 205
Ile His Glu Glu Ile Gln Ala Glu Leu Ser Ile Ile Leu Asn Arg Pro
210 215 220
Asn Gln Ile Ile Lys Gly Ser Ile Asn Ile Gly Lys Glu Val Phe Thr
225 230 235 240
Ile Thr Asn Gln Ala Ala Gln Thr Lys Thr Leu Asp Phe Val Ser Ile
245 250 255
Ser Ala Leu Ser Asp Glu Phe Ile Asn Ala Ser Asp Ser Gln Thr Lys
260 265 270
Gln Ala Ala Leu Arg Ile Gln Gln Lys Gln Lys Glu Leu Ile Pro Leu
275 280 285
Ile Gln Lys Leu Ser Glu Thr Glu Ile Gln Ala Thr Gly Ile Ile Phe
290 295 300
Ile Glu Asp Gln Val Asn Ser Phe Thr Glu Leu Ile Gly Arg Gln Val
305 310 315 320
Ser Thr Leu Asp Tyr Leu Leu Asn Asp Trp Lys Val Leu Asn Ser Asn
325 330 335
Met Asn Gln Ile Lys Leu Asn Leu Glu Thr Glu Lys Tyr Thr Asp Lys
340 345 350
Asn Ser Leu Gln Lys Asp Leu Thr Lys Leu Lys Asn Leu Asn Asp Glu
355 360 365
Ile Asn Lys Gln Thr Asn Gln Tyr Glu Glu Tyr Ile Thr Asn Ile Glu
370 375 380
Val His
385
<210> 131
<211> 383
<212> БЕЛОК
<213> Bacillus sp.
<400> 131
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala
20 25 30
Lys Asn Ala Tyr His Thr Ile Gln Leu Ala Ala Asn Ala Trp Leu Lys
35 40 45
Trp Asp Val Tyr Gly Asn Gly Thr Tyr Asp Asp Ala Gly Ile Leu Leu
50 55 60
Tyr Asn Gly Ser Phe Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ser Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Ala Val
85 90 95
Thr Ile Gly Ala Gly Trp Val Gly Asp Asn Ala Phe Ser Tyr Asn Ser
100 105 110
Glu Gly Leu Lys Gln Ser Lys Trp Thr Gly Ala Ala Ala Asn Lys Trp
115 120 125
Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly
130 135 140
Tyr Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile Thr Gly Pro Gln
145 150 155 160
Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn Asp Arg Leu Phe
165 170 175
His Ile Pro Ala Ala Ala Ser Ser Thr Phe Ser Leu Pro Thr Leu Pro
180 185 190
Asn Val Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Ala Gly Ile Asp
195 200 205
Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu
210 215 220
Ile Pro Cys Ile Met Val Asn Asp Ser Gln Ala Ser Asp Tyr Thr Lys
225 230 235 240
Ile His Thr Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp
245 250 255
Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu Ser Tyr Ser
260 265 270
Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr
275 280 285
Leu Ser Met Lys Phe Gly Ser Asp Leu Gly Ile Lys Phe Gly Asp Val
290 295 300
Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile
305 310 315 320
Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr Thr Ala
325 330 335
Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala
340 345 350
Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly Ser Ile Val Ser Asp Pro
355 360 365
Trp Val Val Lys Asn Asp Lys Ile Thr Ile Thr Arg Lys Thr Ser
370 375 380
<210> 132
<211> 521
<212> БЕЛОК
<213> Bacillus sp.
<400> 132
Met Lys Tyr Asn Glu Arg Gln Lys Ala Lys Arg His Tyr Lys Lys Thr
1 5 10 15
Met Phe Ala Val Ala Ala Ala Leu Ser Ile Gly Val Thr Gly Val Gly
20 25 30
Gly Ala Met Ala Val Pro Thr Ala Ala Tyr Ala Ala Thr Asp Ala Lys
35 40 45
Lys Gly Thr Glu Ile Leu Gln Ile Leu Ser Asp Gly Thr Val Lys Ile
50 55 60
Val Asn Asp Asn Leu Leu Lys Asn Ser Ser Ala Lys Glu Leu Thr Lys
65 70 75 80
Leu Gly Gly Thr Ala Phe Met Gln Leu Val Lys Gly Val Gln Asp Lys
85 90 95
Gly Leu Thr Ser Val Asn Asp Ala Ser Arg Glu Ile Val Leu Ala Gly
100 105 110
Ile Ser Ala Ile Pro Tyr Gly Gly Ala Leu Met Ser Ser Leu Ile Gly
115 120 125
Leu Val Trp Pro Ala Asp Gly Ala Asn Gln Met Arg Gln Leu Met Glu
130 135 140
Glu Met Ser Lys Met Met Asp Gln Lys Ile Glu Asp Phe Asp Lys Ala
145 150 155 160
Gly Leu Asp Arg Gln Ile Lys Asp Ala Gln Asp Ala Leu His Glu Leu
165 170 175
Glu Met Thr Leu Tyr Gly Val Gly Asn Val Gln Asp Gly Phe Phe Ser
180 185 190
Val Gly Asp Leu Gln Asp Thr Ala Arg Arg Lys Ala Glu Ala Ala His
195 200 205
Gly Lys Leu Thr Thr Val Ile Asn Ala Ala Gln Lys Ala Gly Tyr Asp
210 215 220
Lys Ala Glu Leu Pro Val Tyr Thr Ile Ala Val Thr Ala His Leu Met
225 230 235 240
Phe Leu Ser Phe Met Val Glu Asn Gly Glu Ser Pro Leu Phe Asn Ile
245 250 255
Asp Ala Asp Ser Met Asn Arg Thr Tyr Lys Val Tyr Leu Lys Asn Ala
260 265 270
Leu Thr Glu Tyr Glu Thr His Ile Glu Asp Thr Gly Asn Lys Gly Leu
275 280 285
Ser Asp Tyr Asn Gln Gln Ser Gln Leu Ala Lys Asn Ser Gln Asn Glu
290 295 300
Ile Ser Gln Leu Gln Ala Ala Ile Asn Arg Ala Asp Pro Ile Gly Lys
305 310 315 320
Ala Gly Leu Gln Val Thr Leu Asn Lys Ala Ala Thr Ile His Gln Glu
325 330 335
Ile Val Ser Lys Leu Glu Lys Leu Lys Asn Tyr Val Asp Ser Thr Val
340 345 350
Gln Asn Gln Ala Phe Gln Glu Ala Leu Lys Phe Ala Lys Thr Thr Ala
355 360 365
Thr Lys Asn Gly Ile Lys Ile Pro Ser Gln Ser Ala Gln Val Ala Asp
370 375 380
Gly Ile Tyr Ala Ile Ala Ser Ala Leu Asp Thr Thr Lys Leu Val Asp
385 390 395 400
Asn His Thr Asn Thr Lys Asn Val Ser Ile Tyr His Asn Thr Asp Ser
405 410 415
Asn Asn Gln Arg Trp Lys Phe Thr Phe Asp Ser Asn Lys Gln Ala Tyr
420 425 430
Lys Ile Glu Asn Leu Tyr Asp Ser Arg Val Leu Ala Thr Ser Ser Thr
435 440 445
Ala Ile Asn Phe Asn Val Phe Thr Thr Glu Phe Lys Gly Tyr Asp Asp
450 455 460
Gln Phe Trp Thr Leu Glu Pro Ala Gly Asp Arg Asn Tyr Ile Phe Lys
465 470 475 480
Asn Lys Gln Tyr Pro Asp Arg Val Leu Asp Ile Ser Gly Gly Thr Ala
485 490 495
Asn Asp Ser Thr Asn Ile Ile Ala Tyr Gln Ser Thr Asn Pro Gly Ala
500 505 510
Asn Glu Lys Phe Lys Leu Ile Lys Gln
515 520
<210> 133
<211> 396
<212> БЕЛОК
<213> Bacillus sp.
<400> 133
Met Lys Lys Ile Pro Val Lys Leu Met Thr Cys Ala Ala Leu Val Ala
1 5 10 15
Thr Leu Ser Thr Ala Ser Ile Met Pro Ser Tyr Ala Phe Ala Ala Glu
20 25 30
Thr Lys Glu Ala Thr Val Lys Thr Glu Lys Gln Pro Asn Leu Pro Asp
35 40 45
Lys Tyr Leu Gly Pro Glu Gly Leu Lys Lys Ala Leu Glu Asp Thr Gly
50 55 60
Ser His Val Ile Val Leu Asp Ala Tyr Ala Leu Thr Leu Leu Lys Ser
65 70 75 80
Pro Gln Ile Lys Leu Asp Lys Thr Leu Phe Lys Glu Gln Lys Asp Lys
85 90 95
Asp Leu Val Asp Ala Val Ile Asn Ile Gln Lys Val Ala Gln Gly Asn
100 105 110
Ala Thr Gln Trp Val Asp Val Leu Lys Pro Glu Leu Ile Asn Val Asn
115 120 125
Gln Gly Ile Val Asn Tyr Gly Ala Lys Phe Asp Asn Tyr Tyr Gln Thr
130 135 140
Ile Val Asp Ala Val Asp Ala Lys Asp Lys Glu Thr Thr Lys Gln Gly
145 150 155 160
Val Glu Arg Leu Tyr Asn Ser Val Gln Glu Asn Lys Arg Lys Val Glu
165 170 175
Gln Leu Val Glu Asn Leu Lys Asp Phe Arg Asn Lys Leu Ile Thr Asp
180 185 190
Thr Asn Glu Phe Gln Ser Lys Thr Asn Gln Ile Val Thr Asn Leu Glu
195 200 205
Gly Lys Asn Ala Ile Leu Pro Ser Leu Lys Gln Gln Ile Asp Ala Tyr
210 215 220
Asn Ser Gln Val Ser Ala Ser Ile Ala Met Ile Ala Ser Gly Gly Val
225 230 235 240
Leu Thr Leu Met Gly Thr Gly Phe Gly Val Leu Thr Val Val Leu Ala
245 250 255
Val Ser Gly Val Gly Leu Pro Phe Ala Ala Ile Thr Gly Thr Ile Ala
260 265 270
Ala Gly Ala Ile Gly Gly Gly Val Thr Leu Ile Val Thr Gly Asn Asn
275 280 285
Lys Leu Lys Glu Ala Asn Ala Gln Ile Ala Lys Leu Thr Met Gln Leu
290 295 300
Lys Asp Thr Asp Leu Gln Val Ala Ala Leu Lys Val Ala Ser Gly Gln
305 310 315 320
Thr Lys Thr Phe Ala Asp Thr Ile Asn Val Ala Ile Asp Ser Ala Glu
325 330 335
Ala Leu Lys Asp Gln Trp Thr Thr Met Gly Ser Lys Tyr Lys Ser Leu
340 345 350
Ile Asp Asn Ile Asp Gly Ile Ser Pro Ser Ser Leu Ala Phe Ile Lys
355 360 365
Glu Asp Leu Lys Thr Ala Lys Asp Ser Trp Val Asn Ile Lys Thr Tyr
370 375 380
Ala Asp Thr Leu Tyr Ser Lys Ile Thr Ile Gln Lys
385 390 395
<210> 134
<211> 397
<212> БЕЛОК
<213> Bacillus sp.
<400> 134
Leu Leu Arg Ile Ser Asn Leu Lys Arg Arg Ile Ser Met Leu Met Lys
1 5 10 15
Lys Ser Ser Ile Val Thr Gly Cys Val Met Thr Ala Met Leu Ala Ser
20 25 30
Gln Leu Ala Pro Leu Gln Thr Leu Ala Ala Glu Asn Asn Gln Val Val
35 40 45
Glu Gln Ala Ser Thr Ala Thr Val Thr Ser Pro Thr Leu Gln Glu Leu
50 55 60
Gly Thr Gln Ala Val Leu Leu Lys Thr Tyr Ala Leu Thr Leu Leu Lys
65 70 75 80
Gln Pro Thr Val Glu Ile Ala Ala Met Pro Asp Leu Gly Ser Tyr Gln
85 90 95
Glu Lys Ala Lys Gln His Ala Gln Tyr Trp Leu Asp Ile Val Gln Pro
100 105 110
Ser Phe Ile Asp Ala Asn Gln Gln Leu Ile Asp Phe Gln Gln Lys Phe
115 120 125
Gln Asn Tyr Tyr Asp Lys Leu Met Glu Leu Ala Lys Lys Met Gly Thr
130 135 140
Asp Glu Gln Ala Gln Gln Glu Phe Ile Ala Gly Ile Thr Lys Leu Gln
145 150 155 160
Ile Ser Leu Ser Asp His Gln Asn Glu Val Gln Thr Met Ala Ser Asn
165 170 175
Leu Gln Glu Phe Gln Lys Asn Phe Gly Ala Asp Ala Gln Lys Phe Ile
180 185 190
Asp Val Ala Lys Thr Ala Gln Ser Ser Leu Ala Asp Lys Asn Gly Glu
195 200 205
Val Ala Lys Leu Thr Thr Gln Leu Glu Thr Leu Asn Ser Asp Ile Thr
210 215 220
Glu Gln Ile Gly Asn Ile Val Lys Gly Thr Ile Gly Leu Val Ala Gly
225 230 235 240
Ile Gly Ala Ile Thr Phe Gly Thr Ile Val Leu Phe Gly Thr Ala Gly
245 250 255
Val Gly Val Ala Val Ile Ala Pro Ala Leu Gly Gly Ile Val Ala Gly
260 265 270
Thr Thr Gly Leu Val Thr Gly Gly Val Thr Val Gly Phe Ala Ala Lys
275 280 285
Lys Leu Glu Asp Lys Arg Lys Glu Leu Gln Asp Val Val Gln Lys Leu
290 295 300
Thr Ser Ala Lys Ala Asp Ala Ala Ala Leu Thr Leu Leu Thr Gly Gln
305 310 315 320
Ile Asp Thr Phe Lys Lys Thr Leu Glu Lys Gly Lys Asp Ser Val Glu
325 330 335
Asp Phe Gly Thr Ser Cys His Glu Leu Gln Gln Asn Leu Thr Glu Leu
340 345 350
Ser Asn Ser Val Asn Gln Ile His Pro Asp Ser Ala Ile Leu Gln Asn
355 360 365
Arg Leu Ala Gln Ile Lys Lys Ser Val Asp Gly Leu Ala Thr Gln Ala
370 375 380
Lys Gln Gln Glu Gln Thr Ile Thr Asp Ile Ser Tyr Gln
385 390 395
<210> 135
<211> 397
<212> БЕЛОК
<213> Неизвестный
<220>
<223> Неизвестный организм из образца окружающей среды
<400> 135
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Thr Ser Asp Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Ser Ile His Leu Gln Ser Asn Lys Asn Lys Val
35 40 45
Trp Asp Val Phe Asn Asn Ala Thr Tyr Asn Asn Ala Gly Ile Arg Leu
50 55 60
Tyr Gly Gln Thr Asn Gly Asp Asn Gln Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Gly Asp Pro Ile Ile Ile Asp Asp Gly Val Arg Arg Met Thr
100 105 110
Ala Phe Asn Asn Glu Gly Leu Arg Gln Thr Ser Trp Thr Gly Ala Pro
115 120 125
Ala Glu Gln Trp Tyr Leu Arg Glu Gln Gly Thr Ser Asn Tyr Glu Phe
130 135 140
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Trp Met Gly Thr
145 150 155 160
Leu Ala Glu Asn Trp Glu Tyr Val Asp Leu Asp Asn Ser Asn Pro Thr
165 170 175
Asp Gly Asp Arg Val Phe Tyr Val Ser Ala Gly Gln Val Asn Lys Gly
180 185 190
Asn Val Pro Phe His Gly Thr Phe Ser Leu Pro Gln Leu Pro Ala Thr
195 200 205
Gly Thr Arg Pro Asp Ala Pro Ser Tyr Thr Gly Gly Ile Asp Gln Gln
210 215 220
Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro
225 230 235 240
Cys Ile Met Val Asn Asp Ser Gln Thr Ser Asp Tyr Thr Lys Ile His
245 250 255
Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Ala
260 265 270
Ala Ser Thr Val Leu Thr Pro Gly Ala Thr Gln Ser Ile Ser Phe Lys
275 280 285
Thr Gly Met Thr Ser Thr Asp Gln Gln Lys Met Thr Glu Thr Leu Ser
290 295 300
Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Gln Thr Ala
305 310 315 320
Ser Leu Lys Ala Glu Ile Ser Lys Thr Leu Gln Thr Glu Ile Ser Thr
325 330 335
Ser Asn Thr Glu Val Thr Glu Asp Thr Val Thr Thr Ser Leu Thr Gly
340 345 350
Glu Ala Gly Lys Thr Ser Gly Tyr Thr Leu Tyr Gln Leu Ala Thr Lys
355 360 365
Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ala Val Ser Gly Ser Trp Thr
370 375 380
Val Lys Asn Asn Lys Val Thr Val Leu Arg Lys Thr Pro
385 390 395
<210> 136
<211> 386
<212> БЕЛОК
<213> Bacillus sp.
<400> 136
Leu Tyr Leu Asp Phe Ser Ser Phe Trp Arg Lys Pro Arg Gly Asp Ser
1 5 10 15
Asn Gln Ser Ile Ser Phe Met Ile Lys Asn Gly Lys Glu Ile Cys Cys
20 25 30
Met Pro Asn Leu Glu Lys Gly Ile Ala Tyr Asn Ile Ala Leu Gly Thr
35 40 45
Asp Pro Ser Leu Val Phe Asp Ile Leu Gly Asn Ser Thr Glu Phe Gly
50 55 60
Ser Ile Val Gly Leu Tyr Ala Lys Gly Gln Asn Ser Ile Asn Gln Gln
65 70 75 80
Phe Phe Phe Phe Glu Leu Asp Gly Gly Arg Met Ala Ile Val Pro Arg
85 90 95
Asn Ser Gly Arg Pro Leu Glu Pro Gly Phe Gly Ala Thr Pro Asp Thr
100 105 110
Leu Ile Val Gln Gly Glu Trp Thr Gly Leu Ser Ser Gln Gln Trp Gly
115 120 125
Leu Gln Asp Lys Thr Ala Ser Ser Val His Ile Phe Asn Gln Ser Asn
130 135 140
Gly Leu Val Val Ser Tyr Gly Arg Asn Asp Gln Asp Gln Phe Pro Glu
145 150 155 160
Leu Asn Thr Pro Asn Pro Ser Gly Pro Ala Pro Glu Arg Ala Trp Tyr
165 170 175
Leu Glu Pro Ala Tyr Gln Ala Ile Leu Pro Asn Phe Val Pro Thr Thr
180 185 190
Ser Ile Tyr Ser Pro Pro Gln Tyr Ser Ser Gly Ile Tyr Glu Val Leu
195 200 205
Pro Asp Gln Ser Glu Val Ala Thr Val Ser Asn Ala Leu Ile Pro Cys
210 215 220
Ile Leu Val Asn Asp Gly Ala Leu Ser Pro Glu Thr Lys Val Gln Gln
225 230 235 240
Ser Pro Tyr Tyr Leu Leu Ile Lys Gln Gln Phe Trp Gln Lys Ala Ala
245 250 255
Phe Gly Thr Val Ser Pro Asn Ser Ile Arg Glu Phe Ser Phe Lys Ala
260 265 270
Gly Thr Thr Phe Glu Asp Gln Lys Ser Met Ser Asp Thr Ile Asn Met
275 280 285
Thr Val Gly Gly Asp Phe Gly Leu Gln Phe Asn Glu Val Ser Thr Asn
290 295 300
Ala Lys Phe Gly Ile Ser Gln Gln Ile Gln Thr Ala Val Ser Thr Thr
305 310 315 320
Asn Glu Glu Leu Ser Glu Leu Thr Glu Thr Leu Ser Tyr Asn Asn Ile
325 330 335
Gln Lys Asp Thr Leu Val Gly Val Ser Ile Tyr Gln Leu Val Thr Arg
340 345 350
Tyr Val Leu Tyr Arg Ala Asn Gly Ser Leu Val Ala Gln Pro Trp Lys
355 360 365
Phe Ile Asp Asn Ala Ser Ala Val Thr Val Ile Thr Glu Gln Pro Ile
370 375 380
Gly Val
385
<210> 137
<211> 407
<212> БЕЛОК
<213> Bacillus sp.
<400> 137
Met Asn Asp Asn Met Asp Glu Asn Gln Asn Gly Asp Thr Ile Thr Thr
1 5 10 15
Glu Asp Ser Leu Ala Asn Asp Glu Arg Asn Glu Ser Leu Pro Glu Glu
20 25 30
Ser Arg Met Phe Ala Tyr Tyr Gln Pro Val Asn Thr Asn Asp Lys Val
35 40 45
Trp Asp Ala Ser Glu Gly Arg Gly Gly Phe Pro Ile His Ser Cys Glu
50 55 60
His Thr Arg Leu Gly Tyr Ala Met Phe Asn Tyr Pro Tyr His Gly Gly
65 70 75 80
Leu Asn Gln Gln Phe Ile Trp Phe Gln Leu Asp Asp Gly Arg Phe Ala
85 90 95
Ile Val Asn Lys His Asn Gly Leu Val Leu Gln Lys Tyr Gln Val Ser
100 105 110
Asn Thr Ile Arg Glu Ile Val Asn Arg Pro Trp Glu Ser Asn Tyr Ala
115 120 125
Gln His Trp Arg Lys Glu Ser Thr Ser Ala Thr Asn Phe Arg Leu Val
130 135 140
Asn Glu Ala Glu Ala Leu Glu Val Cys Gly Gly Asn Thr Ser Ala Gln
145 150 155 160
Ala Lys Met Ser Phe Ile Pro Asn Arg Pro Asp Asn Ala Leu Leu Phe
165 170 175
Arg Arg Arg Ala Ile Ala Gln Asn Ile Val Val Pro Ala Leu Pro Ser
180 185 190
Lys Gln Pro Leu Pro Ala Leu Pro Ile Leu Ser Asn Leu Asn Asp Pro
195 200 205
Ala Pro His Glu Thr Ala Arg Ala Ile Thr Gly Ser Ser Leu Val Pro
210 215 220
Cys Ile Met Val Ser Asp Tyr Ile Pro Val Ala Gln Arg Ile Lys Asp
225 230 235 240
Ser Pro Tyr Tyr Ile Leu Val Lys Lys Gln Phe Trp Lys Arg Leu Trp
245 250 255
His Asp Thr Phe Ile Pro Gly Glu Tyr Arg Glu Phe Gln Glu Thr Thr
260 265 270
Gly Met Ser Lys Thr His Gln Glu Gly Met Thr Gln Thr Thr Asn Ile
275 280 285
Ser Leu Gln Ala Asp Phe Gly Phe Ala Phe Lys Ala Leu Thr Leu Gly
290 295 300
Leu Gly Val Ser Ile Ser Lys Ser Ile Glu Ile Ser Arg Ser Thr Thr
305 310 315 320
Asp Thr Val Met Ser Gln Tyr Thr Asp Lys Gln Gly Tyr Thr Asn Thr
325 330 335
Lys Asn Phe Asp Leu Ser Trp Ala Lys Tyr Ala Leu Ala Thr Glu Phe
340 345 350
Ile Leu Met Arg Lys Asp Gly Thr Thr Ala Gly Ser Trp Glu Ala Val
355 360 365
Asp Lys Lys Val Thr Arg Ser Ile Ser Tyr Pro Pro Thr Gln Thr Leu
370 375 380
Ser Trp Thr Lys Ser Leu Glu Asp Gln Glu Asp Asn Thr Val Phe Glu
385 390 395 400
Pro Thr Thr Ile Lys Ser Glu
405
<210> 138
<211> 408
<212> БЕЛОК
<213> Pseudomonas sp.
<400> 138
Met Glu Gln Glu Leu Glu Leu Ala Ala Asp Gln Leu Gln Ser Ile Ala
1 5 10 15
Glu Gln Val Val Pro Lys Leu Glu Gly Val Thr Asn Val Val Glu Ala
20 25 30
Ala Lys Phe Val Pro Val Gln Tyr Phe Gly Lys Arg Val Glu Asn Ile
35 40 45
Asp Glu Glu Thr Phe Ile Val Lys Lys Ser His Val Leu Ser Ile Lys
50 55 60
Arg Tyr Val Ser Ala Gly Leu Ala Leu Pro Val Asn Ile Ser Glu Val
65 70 75 80
Glu Ala Phe Leu Gly Tyr Arg Glu Ser Gly Tyr Glu Ala Leu Asp Pro
85 90 95
Ala Ser Met Gln Arg Leu His Lys Asp Ile His Gln His Ala Met Ser
100 105 110
Trp Ala Thr Leu Glu Arg Arg Thr Lys Asp Val Gly Asn Gln Leu Asp
115 120 125
Leu Phe Ala Ser Thr Phe Ile Glu Ser Gly Thr Thr Ile Val Gly Phe
130 135 140
Leu Lys Gly Ser Glu Ala Tyr Lys Asn Phe Gln Gly Asn Val Asp Ser
145 150 155 160
Leu Ser Glu Asp Glu Lys Glu Ser Leu Ala Ala Ile Pro Leu Gly Ser
165 170 175
Thr Asp Ser Lys Arg Val Glu Asn Leu Gly Lys Ala Leu Met Trp Ala
180 185 190
Arg Lys Asp Ile Ala Glu Phe Tyr Leu Asp Val Thr Ser Val Asn Gly
195 200 205
Leu Ala Ile Glu Phe Ser Arg Lys Ile Ser Asp Glu Leu Leu Ile Lys
210 215 220
Val Ala His Lys Gln Lys Ile Val Asp Glu Ala Arg Ile Asp Arg Gly
225 230 235 240
Glu Glu Val Asp Glu Leu Arg Gln Gln Leu Lys Ser Leu Asp Glu Ala
245 250 255
Ile Ala Glu Lys Gln Ser Glu Tyr Asp Ser Gln Val Gly Tyr Ala Phe
260 265 270
Thr Gly Leu Val Phe Gly Pro Ile Gly Leu Leu Val Thr Gly Gly Ile
275 280 285
Phe Gly Ala Gln Ala Glu Glu Thr Arg Ala Gln Lys Asn Leu Leu Leu
290 295 300
Glu Arg Lys Glu Asp Leu Leu Gly Lys Leu Ser Lys Ala Arg Leu Ser
305 310 315 320
Arg Ser Leu Ile Thr Leu Ala Thr Asn Phe Thr Glu Leu Arg Ala Leu
325 330 335
Met Lys Asp Ala Glu Gln Gly Ile Lys Cys Ile Glu Asp Val Leu Gly
340 345 350
Ile Val Trp Ser Ser Leu Gly Val Ser Leu Glu Ala Phe Glu Asp Asn
355 360 365
Ala Thr Glu Leu Asp Leu Ile Leu Leu Val Leu Asn Leu Gln Ala Ile
370 375 380
Phe Glu Pro Trp Lys Thr Ile Lys Gly His Ala Lys Ala Leu Ser Ala
385 390 395 400
Val Phe Asn Gln Ile Val Glu Asp
405
<210> 139
<211> 393
<212> БЕЛОК
<213> Pseudomonas sp.
<400> 139
Met Leu Ala Ile Gln Asp Glu Ile Lys Thr Ala Glu Glu Ile Asn Ser
1 5 10 15
Leu Thr Thr Asp Phe Met Gly Ala Val Ser Gly Thr Leu Arg Gly Val
20 25 30
Glu Arg Gly Pro Gly Leu Leu Val Thr Asn Asp Asp Ile Lys Lys Ile
35 40 45
Lys Arg Tyr Val Asn Ala Gly Leu Ala Leu Pro Ile Asp Ile Asn Glu
50 55 60
Ile Glu Gln Leu Tyr Lys Tyr Asp Gln Leu Asn Ile Ser Gly Leu Tyr
65 70 75 80
Ser Ala Asp Met Gln Ile Leu Tyr Gln Asn Met Lys Asn His Ala Ser
85 90 95
Ser Trp Ser Pro Leu Glu Ser Ser Met Lys Ala Val Gly Ser Asp Leu
100 105 110
His Val Phe Ala Asp Asn Phe Thr Ser Ser Ser Gln Ser Ile Ile Asn
115 120 125
Tyr Leu Thr Arg Leu Pro Ser Tyr Ile Ser Gly Val Gly Lys Ile Gly
130 135 140
Gly Leu Thr Pro Glu Glu Ile Asp Asn Leu Pro Glu Ile Gln Leu Thr
145 150 155 160
Gly Asp Glu Lys Gln Lys Ile Pro Ala Leu Met Glu Leu Ile Glu Glu
165 170 175
Leu Lys Thr Val Ile Thr Glu His Ser Lys Ser Thr Gln Thr Thr Lys
180 185 190
Ser Gln Ile Thr Leu Phe Lys His Glu Ile Thr Asn Ser Leu Lys Pro
195 200 205
Ala Leu Gly Leu Lys Ile Ala Leu Cys Asn Ser His Asn Phe Asp Glu
210 215 220
Glu Ile Lys Ala Phe Asn Glu Arg Leu Asp Ile Leu Asn Gln Arg Ile
225 230 235 240
Asp Glu Lys Tyr Ala Glu Ile Glu Gln Tyr Ser Gln Ser Lys Trp Trp
245 250 255
Gly Leu Phe Gly Gly Ile Val Gly Phe Ile Val Thr Ser Ser Ile Tyr
260 265 270
Gly Thr Lys Ala Arg Gln Ala Arg Asp Glu Leu Glu Ala Leu Ile Ala
275 280 285
Glu Arg Arg Glu Val Gln Ala Lys Ile Ala Ala Ser Ser Ala Val Leu
290 295 300
Ala Ser Leu Arg Ala Phe Glu Thr Ser Leu Gln Glu Leu Gln Ile Arg
305 310 315 320
Ile Glu Asp Ala Ala Gly Ser Ser Ser Asn Leu Glu Ser Leu Trp Glu
325 330 335
Leu Ile Gln Thr Tyr Val Asp Ala Ser Cys Lys Lys Leu Asp Gly Val
340 345 350
Thr Asn Ala Met Tyr Leu Val Ser Phe Val Ser Arg Leu Thr Thr Met
355 360 365
Thr Glu Asn Trp Leu Thr Ile Lys Gln Gln Ala Gly Asp Leu Leu Thr
370 375 380
Ala Phe Asn Asn Ala Thr Ser Ala Ser
385 390
<210> 140
<211> 448
<212> БЕЛОК
<213> Bacillus sp.
<400> 140
Leu Val Leu Ser Phe Tyr Ile Ala Lys Val Ile Leu Phe Arg Asn Lys
1 5 10 15
Ser Ile Ser Asn Val Gln Tyr Asn Phe Ile Phe Cys Asn Leu Val Gly
20 25 30
Ile Leu Pro Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys
35 40 45
Lys Val Met Ala Gly Leu Gly Val Gly Ala Met Ser Leu Ser Ile Trp
50 55 60
Ala Pro Ser Ser Glu Ala Ala Ala Pro Glu Asn Asn Arg Tyr Tyr Ser
65 70 75 80
Ile His Leu Gln Ala Asn Pro Asn Ile Ala Trp Asp Val Tyr Lys Gln
85 90 95
Trp Glu Ser Asp Asn Ala Gly Met Arg Leu Phe Asn Asn Gly Phe Gln
100 105 110
Asp Asn Glu Glu Phe Val Phe Phe Gln Leu Asp Gly Gly Ser Tyr Ala
115 120 125
Ile Val Asn Lys Asn Ser Gly Lys Pro Val Gly Ile Gly Asp Ala Phe
130 135 140
Ile Trp Thr Asp Gly Val Arg Asp Phe Pro Ala Leu Asn Asn Gln Thr
145 150 155 160
Leu Lys Gln Thr Asn Trp Thr Gly Ser Pro Asn Glu Gln Trp Tyr Leu
165 170 175
Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val His Gln Gly Thr Gly
180 185 190
Lys Val Ala Ser His Ala Trp Asn Gln Ile His Gly Pro His Ser Ala
195 200 205
Glu Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Pro His Arg Val
210 215 220
Phe Asn Ile Ala Lys Asn Pro Leu Asn Ala Leu Gly Pro Glu Phe Pro
225 230 235 240
Ile Pro Gly Phe Asp His Gly Thr Phe Ser Leu Pro Thr Leu Pro Ala
245 250 255
Val Gly Thr Arg Pro Lys Ala Pro Asp Tyr Asp Lys Thr Gly Gly Ile
260 265 270
Asp Gln Gln Leu Ser Glu Thr Ser Glu Ser Ala Val Val Gly Ala Ser
275 280 285
Leu Ile Pro Cys Ile Met Val Lys Asp Asn Ser Ala Ser Asp Lys Thr
290 295 300
Lys Ile His Asn Ser Pro Tyr Tyr Val Leu Glu Lys Glu Glu Tyr Trp
305 310 315 320
Glu Lys Val Arg Ser Glu Ile Val Ser Ala Gly Gly Thr Ser Lys Tyr
325 330 335
Thr Val Lys Arg Gly Ile Ser Thr Thr Asp Gln Gln Lys Met Thr Glu
340 345 350
Thr Leu Gly Met Ser Phe Gly Phe Asp Leu Gly Leu Lys Phe Gly Asp
355 360 365
Ser Ser Leu Ala Ile Lys Ser Ser Val Ser Lys Thr Leu Gln Thr Glu
370 375 380
Val Ser Lys Ser Ser Thr Glu Thr Lys Glu Glu Thr Lys Asp Lys Thr
385 390 395 400
Phe Val Ser Gln Ser Asn Lys Asn Thr Gly Leu Thr Val Tyr Gln Leu
405 410 415
Val Thr Arg Tyr Thr Leu Lys Arg Thr Asp Gly Ser Thr Val Ser Pro
420 425 430
Ser Trp Ser Ile Arg Asn Asp Glu Gln Thr Leu Asp Arg Thr Ile Asn
435 440 445
<210> 141
<211> 962
<212> БЕЛОК
<213> Bacillus sp.
<400> 141
Val Ile Leu Pro Gln Ile Asn Asp Gly Ile Thr Gly Lys Gly Arg Ile
1 5 10 15
Leu Leu Phe Arg Glu Leu Lys Gly Gly Asn Ser Met Ala Phe Asp Ile
20 25 30
Gly Ser Phe Tyr Arg Ile Asn Ser Lys Leu Ser Gly Lys Val Phe Asp
35 40 45
Ile Gln Ala Gln Ser Thr Ala Ser Asn Ala Val Val Val Gln Asn Asp
50 55 60
Trp His Asp Arg Asp Ser Gln Lys Phe Ile Phe Tyr Pro Leu Asn Asn
65 70 75 80
Gly Tyr Tyr Thr Val Ile Asn Lys Asn Ser Gly Lys Val Phe Asp Ile
85 90 95
Arg Ala Gln Ser Thr Ala Ser Asn Ala Glu Val Val Gln Asn Asp Trp
100 105 110
His Asp Arg Asp Ser Gln Tyr Phe Phe Thr His Gln Gln Glu Ser Gly
115 120 125
Phe Phe Ser Val Gln Ser Arg Leu Ser Gly Lys Val Phe Asp Ile Arg
130 135 140
Ala Gln Ser Thr Ala Ser Asn Ala Val Val Val Gln Asn Asp Trp His
145 150 155 160
Asp Arg Asp Ser Gln Lys Phe Ser Phe Ser Pro Val Glu Ser Ile Ser
165 170 175
Val Pro Ser Ile Gln Thr Gln Pro Leu Pro Pro Pro Pro Phe Tyr Asn
180 185 190
Gly Ile Asn Asp Ile Leu Pro Asp Arg Thr Glu Pro Val Val Thr Asn
195 200 205
Tyr Thr Leu Ala Pro Cys Ile Ala Val Asp Asp Pro Tyr Tyr Asn Asp
210 215 220
Gln Gln Lys Ile Gln Thr Gly Asn Glu Tyr Tyr Leu Tyr Val Arg Glu
225 230 235 240
Gln Tyr Trp Gln Arg Val Phe Ser His Val Leu Val Pro Gly Glu Ser
245 250 255
Tyr Thr Tyr Ser Gln Thr Ser Gly Met Thr Thr Thr Asp Gln Asn Thr
260 265 270
Val Thr Asn Thr Val Ser His Ser Ile Gly Ala Asp Val Gly Phe Ser
275 280 285
Phe Lys Gln Gly Ser Leu Ser Ala Gly Leu Ser Tyr Gln Tyr Thr Gln
290 295 300
Glu Leu Ser Val Thr Glu Ser His Thr Glu Glu Leu Leu Thr Ser Thr
305 310 315 320
Thr Lys Thr Phe Glu Ile Asn Asn Asn Ser Arg Glu Pro Val Ala Tyr
325 330 335
Ser Gln Tyr Leu Leu Val Ser Glu Tyr Tyr Val Arg Arg Ala Asp Gly
340 345 350
Thr Ile Val Asn Asp Arg Trp Ala Val Ser Asp Ala Asp Ser Thr Val
355 360 365
Thr Val Ser Tyr Pro Ser Asn Val Glu Leu Pro Ile Leu Glu Leu Asp
370 375 380
Ser Gly Phe Val Asp Ala Gln Gln Ala Val Arg Asn Leu Phe Ala Asp
385 390 395 400
Ala Glu His Ser Arg Leu Lys Ile Asp Thr Thr Asp Tyr Met Ile Asp
405 410 415
Gln Ala Ala Leu Gln Val Glu Gln Leu Ser Asp Glu Ser Asp Glu Leu
420 425 430
Glu Lys Met Met Leu Phe Asn Val Val Arg Cys Ala Lys Gln Val Ser
435 440 445
Lys Lys Arg Asn Leu Leu Arg Asp Gly Asp Phe Glu Ser Pro Asn Trp
450 455 460
Gln Asn Pro Glu Gly Gly Trp Gln Ala Ser Tyr Asn Val Thr Val Leu
465 470 475 480
Ser Asp Asn Pro Ile Phe Lys Gly Asn Tyr Val Asp Met Pro Gly Ala
485 490 495
Asn Ala Arg Asn Gly Ser Ile Thr Pro Thr Tyr Leu Tyr Gln Lys Val
500 505 510
Asp Glu Ser Lys Leu Lys Pro Tyr Thr Arg Tyr Leu Val Arg Gly Phe
515 520 525
Val Gly Ser Ser Lys Glu Leu Glu Leu Phe Val Thr Arg Tyr Gly Lys
530 535 540
Glu Val His Asp Lys Met Asn Ile Pro Thr Ser Pro Met Asn Thr Cys
545 550 555 560
Asn Gln Thr Asp Asn Tyr Ser Leu Gly Tyr Gly Ser Gly Tyr Thr Met
565 570 575
Ser Asn Asp Pro Cys Gln Leu Tyr Pro Thr Asn Pro Met Pro Ser Ser
580 585 590
Asn Gly Leu Cys Glu Glu Lys Gln Asn Phe Val Phe His Ile Asp Val
595 600 605
Gly Glu Ile Asn Pro Arg Ala Asp Leu Gly Ile Gly Val Gly Phe Asn
610 615 620
Ile Ser Ser Pro Asp Gly Met Ala Gln Leu Ser Asn Val Glu Val Ile
625 630 635 640
Glu Ala Asn Pro Leu Thr Gly Glu Ala Leu Ala Arg Val Lys Lys Arg
645 650 655
Glu Gln Lys Trp Arg Arg Glu Met Glu Lys Lys Cys Ala Leu Thr Glu
660 665 670
Lys Thr Val Ser Val Ala Thr Gln Ala Val His Arg Leu Phe Thr Asp
675 680 685
Ala Gln Lys Asn Arg Leu Lys Ala Thr Thr Thr Met Gln Asp Ile Arg
690 695 700
Asn Ala Glu Ala Lys Val Lys Ala Ile Pro Phe Val Tyr Asn Thr Tyr
705 710 715 720
Phe Glu Glu Val Pro Gly Met Asn Asp Ala Ile Phe Gln Ala Leu Gln
725 730 735
Ser Asp Val Tyr Thr Ala Ser Gly Leu Tyr Ile Asp Arg Asn Ile Ile
740 745 750
Arg Asn Gly Asp Phe Gly Ser Arg Leu Ser Asn Trp His Ala Thr Ala
755 760 765
Gly Ala Asp Val Gln Asp Arg Asp Gly Arg Ser His Val Leu Val Ile
770 775 780
Ser Asn Trp Ser Ala Asn Val Ser Gln Glu Val Cys Val Gln Pro Glu
785 790 795 800
Arg Gly Tyr Val Leu Arg Val Thr Ala Arg Lys Glu Gly Ser Gly Lys
805 810 815
Gly Tyr Val Thr Leu Thr Asp Cys Thr Ala Glu Asn Thr Glu Thr Val
820 825 830
Thr Phe Thr Ala Asn Glu Asp Val Thr Ile Pro Arg Pro Pro Val Lys
835 840 845
Pro Arg Gln Ser Val Thr Ser Pro Val Cys Asp Met Ser Arg Tyr Ala
850 855 860
Glu Ser Phe Gly Ile Val Pro Asp Asp Thr Asn Met Met Asn Arg Ser
865 870 875 880
Gln Ala Ser Tyr Gly Thr Ala Ser Cys Ser Cys Gly Cys Gly Lys Thr
885 890 895
Ile Ser Asn Gly Glu Gly Ser Cys Gln Thr Asn Pro Cys Pro Ser Thr
900 905 910
Pro Ser Met Pro Ile Gly Asn Gly Pro Ala Ser Asn Tyr Ile Thr Lys
915 920 925
Thr Ile Glu Ile Phe Pro Glu Thr Asn Arg Val Arg Ile Glu Ile Gly
930 935 940
Glu Thr Glu Gly Ser Phe Leu Val Glu Ser Val Glu Leu Ile Cys Met
945 950 955 960
Glu Glu
<210> 142
<211> 353
<212> БЕЛОК
<213> Bacillus sp.
<400> 142
Met Val Lys Arg Asp Ile Ser Ala Arg Asp Leu Gly Gly Lys Glu Asp
1 5 10 15
Thr Pro Phe Leu Leu Lys Lys Glu Glu Trp Ile Lys Ile Gln Lys Tyr
20 25 30
Thr Gly Asp Gly Ala Tyr Leu Pro Val Asn Val Asn Glu Met Arg Lys
35 40 45
Ala Leu Leu Leu Pro Asn Thr Glu Glu Leu Pro Asp Phe Gly Glu Leu
50 55 60
Tyr Thr Val Asn Thr Ser Ile Lys Gly His Cys Lys Asn Trp Asn Asp
65 70 75 80
Thr Thr Tyr Arg Glu Ile Leu Asp Val Ala Asn Gln Ile Val Asn Tyr
85 90 95
Ser Arg Arg Ala Glu Val Phe Tyr Ala Pro Leu Leu Asp Tyr Leu Pro
100 105 110
Asp Ile Leu Glu Gly Asp Thr Val Ala Leu Glu Lys Phe Gln Lys Ile
115 120 125
Cys Lys Lys Leu Ala Asp Glu Ala Lys Asp Phe Ser Asp His Ala Glu
130 135 140
Thr Leu Ala Lys Asn Val Lys Gln Phe Ala Asp Asp Thr Thr Ile Asp
145 150 155 160
Tyr Asn Asn Leu Met Asn Val Lys Ala Lys Tyr Asp Glu Leu Tyr Gly
165 170 175
Lys Asn Ser Glu Glu Glu Ile Arg Leu Arg Ala Glu Val Glu Lys Leu
180 185 190
Arg Lys Glu Leu Glu Glu Tyr Ile Asp Asp Tyr Glu Asp Phe Glu Lys
195 200 205
Glu Val Trp Leu Ser Leu Leu Leu Gly Pro Leu Phe Gly Phe Ile Leu
210 215 220
Lys Glu Ile Leu Glu Ser Thr His Gly Lys Met Leu Leu Ala Lys Ile
225 230 235 240
Glu Ala Thr Lys Gln Lys Ile Glu Ala Thr Cys Ala Thr Ile Gln Arg
245 250 255
Asn Val Tyr Leu Met Ser Leu Leu Asp Lys Ala Asp Val Gly Thr Asp
260 265 270
Lys Thr Gln Lys Gln Met Lys Glu Ala Leu Pro Val Ile Asn Lys Ile
275 280 285
Lys Ala Ile Trp Ser Ser Leu His Lys Asp Leu Leu Thr Leu Ser Thr
290 295 300
Ile Thr Met Lys Asp Ile His Asp Asp Pro Glu Phe Ala Asp Leu Gly
305 310 315 320
Ile Glu Leu Ala Ile Met Gln Trp Val Lys Val Gly Lys Gln Ala Asp
325 330 335
Asp Phe Arg Ile Asn Ala Asp Val Gly Phe Ile Val Asp Gln Tyr Ile
340 345 350
Ala
<210> 143
<211> 367
<212> БЕЛОК
<213> Bacillus sp.
<400> 143
Met Glu Phe Lys Ala Gly Lys Met Tyr Arg Phe Lys Asn Lys Leu Ser
1 5 10 15
Gly Lys Tyr Leu Asp Val Ala Arg Asp Ser Lys Asp Glu Trp Ala Asn
20 25 30
Ile Gln Gln Tyr Glu Tyr Val Lys Asp Ser Leu Ser Glu Lys Phe Phe
35 40 45
Ile His Pro Leu Asp Asn Asn Tyr Tyr Ala Ile Ile Ser Cys Leu Ser
50 55 60
Gly Arg Thr Leu Asp Ile Ala Asn Asp Ser Lys Asp Asn Trp Ala Asn
65 70 75 80
Val Gln Leu Phe His Trp Thr Gly Gly Asp Ser Glu Ile Trp Ser Leu
85 90 95
Asn Ala Asp Pro Asn Asn Pro Asn Tyr His Val Val Met Ser Asn Leu
100 105 110
Ser Gly Lys Val Leu Asp Val Ala Arg Asp Ser Lys Asp Asn Trp Ala
115 120 125
Asn Ile Gln Met Tyr Glu Glu Asn Pro Asn Leu Asp Gly Gln Lys Trp
130 135 140
Leu Ile Glu Glu Asp Gly Glu Val Ile Ile Pro Thr Ile Glu Thr Glu
145 150 155 160
Ala Leu Pro Ser Val Pro Gly Cys Thr Lys Ile Asn Glu Val Leu Pro
165 170 175
Asp Lys Thr Arg Lys Val Ala Thr His Thr Thr Thr Ile Pro Ala Ile
180 185 190
Leu Val Gln Asp Ser Ile Tyr Ser Leu Ala Glu Gln Ile Thr Lys Ser
195 200 205
Pro Tyr Tyr Asn Ile Val Lys Ser Gln Tyr Trp Arg Lys Ile Ala Asp
210 215 220
Tyr Arg Met Ala Pro Gly Glu Ser His Glu Val Thr Asn Thr Thr Gly
225 230 235 240
Ile Thr Glu Thr Asp Gln Gln Thr Ile Lys His Thr Ile Gly Ile Ser
245 250 255
Ile Gln His Asp Met Gly Ile Ser Phe Glu Gly Val Ser Ala Ser Ser
260 265 270
Ser Thr Gln Phe Arg Tyr Glu Leu Gly Ile Glu Asn Ser His Thr Thr
275 280 285
Glu Lys Leu Lys Gln Glu Thr Gln Arg His Lys Ile Val Asn Ile Lys
290 295 300
Asp Lys Asp Met Arg Phe Val Gln Tyr Ile Leu Glu Thr Glu Tyr Ser
305 310 315 320
Leu Arg Arg Ser Asn Gly Ser Ile Val Lys Thr Trp Ser Leu Asn Asp
325 330 335
Lys Asn Thr Val His Ser Val Ser Tyr Phe Gln Glu Asp Ser Glu Asn
340 345 350
Ala Asn Val Val Ala Arg Ile Ser Asp Ile Glu Thr Ile Ser Ser
355 360 365
<210> 144
<211> 383
<212> БЕЛОК
<213> Bacillus sp.
<400> 144
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala
20 25 30
Lys Asn Ala Tyr His Ser Ile His Leu Ala Ala Asn Ala Asn Leu Lys
35 40 45
Trp Asp Val Tyr Gly Ser Ser Pro Asn Asp Glu Ala Gly Ile Ala Leu
50 55 60
Tyr Asn Gly Ser Leu Arg Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Ala Val
85 90 95
Thr Ile Gly Ala Gly Trp Val Gly Asp Asn Ala Phe Ser Tyr Asn Ser
100 105 110
Glu Gly Leu Lys Gln Ser Lys Trp Thr Gly Ala Ala Ala Asn Lys Trp
115 120 125
Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly
130 135 140
Tyr Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile Thr Gly Pro Gln
145 150 155 160
Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn Asp Arg Leu Phe
165 170 175
His Ile Pro Ala Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu Pro
180 185 190
Thr Ile Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Ile Asp
195 200 205
Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu
210 215 220
Leu Pro Cys Ile Met Val Asn Asp Ser Gln Val Asp Asp Tyr Thr Lys
225 230 235 240
Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp
245 250 255
Lys Thr Phe Ser Lys Val Val Gln Pro Gly Leu Ser Glu Ser Tyr Ser
260 265 270
Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr
275 280 285
Leu Ser Met Lys Phe Gly Ala Asp Leu Gly Ile Lys Phe Gly Asp Val
290 295 300
Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile
305 310 315 320
Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr Thr Ala
325 330 335
Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala
340 345 350
Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Thr Val Ser Asp Pro
355 360 365
Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys Thr Ser
370 375 380
<210> 145
<211> 495
<212> БЕЛОК
<213> Bacillus sp.
<400> 145
Met Ala Asn Asp Tyr Leu Pro Ser Val Val Ser Ile Glu Leu Asp Ala
1 5 10 15
Leu Glu Lys Met Val Glu Lys Val Val Thr Ser Lys Asn Ile Lys Phe
20 25 30
Phe Ile Lys Pro Ser Lys Thr Val Ile Asn Asp Ile Val Lys Ala Ala
35 40 45
His Thr Asp Gln Leu Asn Phe Gln Thr Ile Phe Arg Ser Phe Ala Ile
50 55 60
Thr Ala Ser Ser Leu Ile Pro Tyr Gly Gly Gly Phe Ile Ala Pro Leu
65 70 75 80
Leu Glu Leu Val Trp Pro Asn Ala Asp Ile Val Lys Glu Lys Asp Asn
85 90 95
Gln Leu Ile Glu Leu Phe Ser Lys Ile Ala Lys Glu Thr Val Asp Glu
100 105 110
Ala Ile Lys Thr Phe Asp Ser Ala Met Ile Thr Ala Thr Leu Lys Thr
115 120 125
Leu Lys Asp Glu Tyr Ser Glu Leu Glu Ser Glu Phe Asn Asn Thr Thr
130 135 140
Asp Tyr Tyr Ser Ile Gly Thr Arg Ala Glu Arg Asn Arg Thr Lys Val
145 150 155 160
Glu Ile Ile Asn Ser Asn Phe Lys Leu Ala Leu Asn Gln Ala Ser Lys
165 170 175
Ser Gly Phe Glu His Ala Glu Leu Pro Phe Tyr Thr Ala Ile Ala Thr
180 185 190
Val Tyr Leu Leu Phe Ile Lys Ser Ile Lys Thr Asn Gly Lys Gly Ser
195 200 205
Lys Leu Leu Leu Asp Asp Ile Thr Tyr Gln Glu Tyr Val Asn Leu Leu
210 215 220
Pro Asn Phe Ser Asn Thr Ser Ile Asn His Ile Arg Lys Ile Phe Phe
225 230 235 240
Asp Tyr Val Glu Lys Asn Ser Phe Pro Thr Arg Glu Tyr Tyr Tyr Ser
245 250 255
Thr Ile Arg Asn Ala Thr Phe Trp Arg Val Leu Glu Leu Asn Thr Phe
260 265 270
Ile Gln Glu Asn Gly Lys Thr Phe Tyr Leu Asp Asp Lys Gly Arg Lys
275 280 285
Phe Thr Gly Trp Tyr Ser Asp Arg Thr Ser Ser His Ala Tyr Phe Pro
290 295 300
Tyr Leu Glu Ser Asn Ala Tyr Tyr Tyr Phe Ser Asp Lys Lys Thr Asp
305 310 315 320
Lys Phe Glu Leu Gly Glu Met Val Thr Gly Phe Phe Thr Asp Thr Asp
325 330 335
Gly Arg Glu Tyr Tyr Leu Ser Glu Lys Asp Asn Leu Ser Met Tyr Val
340 345 350
Ser Pro Glu Pro Ser Asn Val Ser Pro Phe Arg Thr Leu Asn Lys Gly
355 360 365
Glu Leu Ala Val Gly Tyr Phe Glu Leu Glu Gly Lys Arg Ser Phe Leu
370 375 380
Ala Thr Met Pro Ile Leu His Pro Pro Glu Phe Lys Glu Gly Glu Leu
385 390 395 400
Ile Thr Arg Trp Val Gln Asp Ser His Phe His Tyr Tyr Tyr Phe Ala
405 410 415
Pro Lys Asp Gly Thr Leu Asn Asp Ala Gly Glu Lys Phe Arg Lys Gly
420 425 430
Gln Met Met Thr Gly Trp Ile Trp Asp Asp Gln Asn Arg Gly Trp Tyr
435 440 445
Tyr Leu Thr Glu Asn Thr Ile Thr Asn Met Asp His Thr Thr Phe Lys
450 455 460
Arg Gly Gln Met Val Thr Gly Asn Ile Ile Leu Ser Gly Ser Pro Tyr
465 470 475 480
Asn Phe Asp Ser Thr Gly Lys Cys Ile Asn Cys Lys Pro Lys Lys
485 490 495
<210> 146
<211> 327
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 146
Met Ala Asn Asp Tyr Leu Pro Ser Val Val Ser Ile Glu Leu Asp Ala
1 5 10 15
Leu Glu Lys Met Val Glu Lys Val Val Thr Ser Lys Asn Ile Lys Phe
20 25 30
Phe Ile Lys Pro Ser Lys Thr Val Ile Asn Asp Ile Val Lys Ala Ala
35 40 45
His Thr Asp Gln Leu Asn Phe Gln Thr Ile Phe Arg Ser Phe Ala Ile
50 55 60
Thr Ala Ser Ser Leu Ile Pro Tyr Gly Gly Gly Phe Ile Ala Pro Leu
65 70 75 80
Leu Glu Leu Val Trp Pro Asn Ala Asp Ile Val Lys Glu Lys Asp Asn
85 90 95
Gln Leu Ile Glu Leu Phe Ser Lys Ile Ala Lys Glu Thr Val Asp Glu
100 105 110
Ala Ile Lys Thr Phe Asp Ser Ala Met Ile Thr Ala Thr Leu Lys Thr
115 120 125
Leu Lys Asp Glu Tyr Ser Glu Leu Glu Ser Glu Phe Asn Asn Thr Thr
130 135 140
Asp Tyr Tyr Ser Ile Gly Thr Arg Ala Glu Arg Asn Arg Thr Lys Val
145 150 155 160
Glu Ile Ile Asn Ser Asn Phe Lys Leu Ala Leu Asn Gln Ala Ser Lys
165 170 175
Ser Gly Phe Glu His Ala Glu Leu Pro Phe Tyr Thr Ala Ile Ala Thr
180 185 190
Val Tyr Leu Leu Phe Ile Lys Ser Ile Lys Thr Asn Gly Lys Gly Ser
195 200 205
Lys Leu Leu Leu Asp Asp Ile Thr Tyr Gln Glu Tyr Val Asn Leu Leu
210 215 220
Pro Asn Phe Ser Asn Thr Ser Ile Asn His Ile Arg Lys Ile Phe Phe
225 230 235 240
Asp Tyr Val Glu Lys Asn Ser Phe Pro Thr Arg Glu Tyr Tyr Tyr Ser
245 250 255
Thr Ile Arg Asn Ala Thr Phe Trp Arg Val Leu Glu Leu Asn Thr Phe
260 265 270
Ile Gln Glu Asn Gly Lys Thr Phe Tyr Leu Asp Asp Lys Gly Arg Lys
275 280 285
Phe Thr Gly Trp Tyr Ser Asp Arg Thr Ser Ser His Ala Tyr Phe Pro
290 295 300
Tyr Leu Glu Ser Asn Ala Tyr Tyr Tyr Phe Ser Asp Lys Lys Thr Asp
305 310 315 320
Lys Phe Glu Leu Gly Glu Met
325
<210> 147
<211> 373
<212> БЕЛОК
<213> Bacillus sp.
<400> 147
Val Gly Lys Ser Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Ser Gly Asn Glu Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Lys Asp Ala Pro Ser
35 40 45
Glu Arg Phe Phe Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Ile Ser Gly Asn Glu Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Phe Glu Trp Leu Gly Asp Ala Pro Ser
85 90 95
Glu Tyr Trp Tyr Phe His Arg Glu Ala Asp Gly Tyr Tyr Val Ile Glu
100 105 110
Ser Lys His Ser Gly Lys Val Leu Asp Ile Ser Gly Asn Glu Thr Gly
115 120 125
Asn Asn Ala Asn Val Gln Gln Phe Glu Phe Leu Ala Asp Ala Pro Ser
130 135 140
Glu Arg Phe Ala Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Asn Thr Gln Pro Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Glu Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val
180 185 190
Pro Ala Ile Ser Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr
225 230 235 240
Thr Ser Lys Thr Gly Ile Lys Val Thr Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Trp Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly
260 265 270
Phe Ser Val Gly Met Ser Ser Gln Tyr Ser Thr Gln Leu Gln Thr Ser
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu
290 295 300
Ile Lys Asn Pro Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Ser Val Gln Arg Lys Asn Gly Thr Ile Val Asn Ser
325 330 335
Pro Trp Thr Met Thr Asp Lys Thr Arg Thr His Ala Val Thr Tyr Pro
340 345 350
Asn Ala Glu Glu Lys Ala Leu Asn Glu Asn Lys Lys Gln Leu Ser Lys
355 360 365
Ala Glu Ser Val Asn
370
<210> 148
<211> 374
<212> БЕЛОК
<213> Bacillus sp.
<400> 148
Val Ala Lys Ser Met Thr Phe Lys Val Gly Thr Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Ala Gly Asn Arg Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Glu Lys Ala Asp Ser
35 40 45
Glu Arg Phe Phe Leu His Lys Leu Asp Asp Asp Tyr Tyr Ala Met Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Ile Ser Gly Asn Glu Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ala Ser Ser
85 90 95
Gln Tyr Trp Lys Phe Ile Pro Glu Thr Glu Gly Tyr Tyr Val Ile Lys
100 105 110
Ser Lys Leu Ser Gly Lys Val Leu Asp Ile Ser Gly Asn Arg Thr Asp
115 120 125
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Glu Lys Ala Asp Ser
130 135 140
Glu Arg Phe Ala Val Glu Glu Ala Gly Ser Val Pro Leu Pro Ser Ile
145 150 155 160
Asp Lys Lys Pro Leu Ser Pro Val Pro Gln Tyr Lys Thr Ile Asn Asp
165 170 175
Gln Leu Pro Glu Glu Thr Glu His Val Val Arg Ala Phe Ala Arg Val
180 185 190
Pro Ala Ile Ser Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Gln Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys His
225 230 235 240
Thr Val Arg Thr Gly Ile Lys Val Ile Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Trp Ser Ile Gly Ala Asp Met Gly Leu Asp Phe Lys Gly
260 265 270
Phe Ser Ala Gly Met Ser Ser Gln Tyr Ser Glu Gln Leu Glu Thr Thr
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Ile Met Asp His Glu
290 295 300
Ile Arg Asn Thr Thr Thr Asp Asn Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Val Thr Glu Tyr Ser Val Ile Arg Pro Lys Asp Gly Ser Thr Val Asn
325 330 335
Ser Ser Trp Thr Met Thr Asp Lys Asn Asn Ala His Ala Val Tyr Phe
340 345 350
Pro Lys Ser Ile Gly Asn Leu Leu Asn Glu Ser Thr Lys Gln Leu Ser
355 360 365
Lys Thr Glu Lys Val Lys
370
<210> 149
<211> 446
<212> БЕЛОК
<213> Bacillus sp.
<400> 149
Leu Val Leu Ser Phe Tyr Ile Ala Lys Val Ile Leu Phe Arg Asn Lys
1 5 10 15
Ser Ile Ser Asn Val Gln Tyr Asn Ile Ile Phe Cys Asn Leu Val Gly
20 25 30
Ile Leu Pro Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys
35 40 45
Lys Val Met Ala Gly Leu Ala Val Gly Ala Met Ser Leu Ser Ile Trp
50 55 60
Ala Pro Ala Ser Glu Ala Ala Ser Pro Glu Lys Asn Arg Tyr Tyr Ser
65 70 75 80
Ile His Leu Lys Ala Asn Pro Lys Leu Ala Trp Asp Val Ser Gly Asn
85 90 95
Ser Leu Asp Asn Asp Arg Ala Ile Val Leu Trp Asn Thr Ala Ser Arg
100 105 110
Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu Asp Gly Gln Asn Tyr
115 120 125
Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val Thr Phe Gly Ala Gly
130 135 140
Ala Leu Leu Glu Gly His Asn Gly Ile Met Asp Gly Gln Gly Leu Lys
145 150 155 160
Gln Lys Ser Trp Thr Gly Ala Ala Thr Glu Gln Trp Tyr Leu Arg Asp
165 170 175
Gln Gly Ser Asn Asn Tyr Glu Ile Val Asn Gln Gly Asn Gln Lys Val
180 185 190
Ala Ser Tyr Gly Trp Gln Gly Ser Leu Ala Asp Tyr Ala Glu Tyr Val
195 200 205
Lys Leu Asp Glu Ser Lys Pro Ser Asp Ser Asn Arg Val Phe Asn Ile
210 215 220
Ala Lys Asn Gly Phe Gly Gly Val Gly Gly Asp Ile Gln Ile Pro Gly
225 230 235 240
Phe Asn Leu Asp Tyr Gly Thr Phe Ser Leu Pro Thr Leu Pro Ala Thr
245 250 255
Gly Thr Arg Pro Ala Ala Pro Asp Tyr Thr Thr Ala Asp Pro Asn Gln
260 265 270
Gln Leu Pro Gln Ala Ser Asn Ser Val Val Val Gly Ala Ser Leu Val
275 280 285
Pro Cys Ile Met Val Asn Asp Ser Gln Thr Ser Asp Tyr Thr Lys Ile
290 295 300
His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys
305 310 315 320
Ala Ala Ser Thr Val Leu Thr Pro Gly Ala Thr Gln Ser Val Ala Phe
325 330 335
Lys Thr Gly Met Ser Ser Ser Asp Gln Gln Lys Met Thr Glu Thr Leu
340 345 350
Ser Met Lys Val Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Lys Thr
355 360 365
Ala Ala Leu Lys Ala Glu Ile Thr Lys Thr Leu Gln Thr Glu Thr Ser
370 375 380
Thr Thr Asn Thr Glu Leu Ser Glu Asp Thr Val Thr His Asn Phe Lys
385 390 395 400
Asp Glu Val Gly Lys Asp Thr Gly Tyr Thr Leu Tyr Gln Leu Ala Thr
405 410 415
Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Val Val Ser Gly Ser Trp
420 425 430
Thr Val Lys Asn Asn Lys Ile Thr Lys Val Ser Lys Ser Lys
435 440 445
<210> 150
<211> 502
<212> БЕЛОК
<213> Bacillus sp.
<400> 150
Leu Met Lys Tyr Lys Asp Arg Lys His Ala Lys His Lys Tyr Lys Gln
1 5 10 15
Ala Leu Leu Ala Thr Val Ala Thr Met Thr Phe Gly Val Ser Thr Leu
20 25 30
Gly Ser Thr Ala Ser Ala Phe Ala Glu Glu Lys Asp Lys Asn Ala Thr
35 40 45
Gln Gln Gln Asn Thr Val Gln Gln Lys Asp Ala Gly Thr Tyr Tyr Glu
50 55 60
Gly Glu Gln Lys Glu Leu Asn Pro Leu Ala Gln Leu Asp Lys Trp Thr
65 70 75 80
Gln Asp Leu Gly Lys Ala Ser Gly Ala Gly Asp Tyr Lys Thr Thr Leu
85 90 95
Gly Met Val Glu Lys Leu Leu Pro Thr Ile Tyr Lys Asp Leu Asn Ser
100 105 110
Gly Asn Phe Asn Asn Thr Ala Arg Ser Phe Thr Met Leu Ser Thr Ala
115 120 125
Leu Ile Pro Tyr Gly Gly Ala Phe Ile Ser Pro Ile Val Gly Leu Ile
130 135 140
Trp Pro Glu Asn Thr Thr Ala Gln Asp Ala Lys Leu Gln Lys Met Val
145 150 155 160
Glu Gly Leu Thr Ser Met Met Gly Gln Lys Ile Asp Asp Tyr Asp Leu
165 170 175
Ala Lys Leu Lys Gln Lys Thr Asp Ala Leu Thr Asp Thr Leu Lys Lys
180 185 190
Phe Gln Asn Ser Val Asn Asn Lys Pro Gln Ser Glu Ser Tyr Tyr Asp
195 200 205
Asn Ile Gln Thr Ala Asn Val Thr Tyr Ala Asp Tyr Ile Asn Thr Lys
210 215 220
Phe Glu Glu Ile Ile Lys Glu Ser Gln Thr Pro Gly His Gln Ile Pro
225 230 235 240
Glu Leu Pro Ile Phe Thr Val Ile Ala Thr Ala His Leu Gln Phe Leu
245 250 255
His Phe Ile Glu Glu Asn Ala Val Lys Asn Pro Arg Ile Lys Met Asp
260 265 270
Pro Glu His Leu Asn Arg Tyr Phe Pro Lys Phe Lys Asp Thr Ala Glu
275 280 285
Lys Tyr Arg Lys Tyr Ile Asn Asn Val Asn His Asp Ala Gln Gln Trp
290 295 300
Leu Tyr Asp Phe Thr Gly Gly Gly His Ile Lys Ala Asn Asn Pro Ala
305 310 315 320
His Ile Glu Ala Met Asn Lys Ile Ala Asp Leu Thr Lys Gln Thr Ser
325 330 335
Asp Asn Glu Ala Phe Leu Leu Ala Ser Asn Pro Pro Ser Ala Gly Asp
340 345 350
Lys Thr Gly Leu Val Lys Asp Gly Asp Lys Trp Asn Tyr Tyr Ser Pro
355 360 365
Arg Asp Gly Phe Glu Asn Thr Gln His Gln Lys Phe Asn Lys Gly Ser
370 375 380
Leu Val Thr Gly Pro Leu Asp Ile Asp Gly Arg Thr Tyr Leu Phe Ser
385 390 395 400
Phe Glu Glu Gly Met Lys Asn Ser Ala Gly Lys Lys Phe Asn Lys Gly
405 410 415
Asp Met Phe Thr Gly Trp Tyr Asn Asp Ala Gly Val Trp Ser Tyr Tyr
420 425 430
Lys Pro Glu Ser Gly Val Met Ala Thr Gly Trp Gln Asn Ile Ser Gly
435 440 445
Lys Trp Tyr Tyr Phe Ala Thr Ala Tyr Gln Glu Phe Asn Gly Gly Ala
450 455 460
Phe Ile Ser Lys Gly Asp Met Ile Lys Gly Lys Thr Val Glu Ile Ser
465 470 475 480
Gly Lys Leu Tyr Thr Phe Asp Glu Asn Gly Ala Cys Leu Asn Pro Asp
485 490 495
Gly Lys Pro Ile Ala Lys
500
<210> 151
<211> 376
<212> БЕЛОК
<213> Bacillus sp.
<400> 151
Val Met Ser Asn Glu Ile Lys Asn Asn Glu Asp Met Asn Lys Pro Met
1 5 10 15
Ile Asn Asn Glu Asp Ser Ile Gln Ser Glu Ala Lys Ala Ser Lys Gly
20 25 30
Leu Ile Ala Phe Pro Asp Phe Asn Tyr Tyr Asn Ile Arg Thr Phe Val
35 40 45
Gly Lys Tyr Ala Asp Val Thr Gly Gly Ala Gly Asn Gly Arg Pro Val
50 55 60
Val Gln Tyr His Pro Asn Gly Ser Asp Asn Gln Lys Phe Leu Ile Phe
65 70 75 80
Thr Leu Asp Asn Gly Tyr Ser Val Ile Ala Val Lys His Ser Gly Lys
85 90 95
Val Leu Asp Leu Ala Tyr Asn Ser Ser Gly Asp Arg Leu Ile Gln Tyr
100 105 110
Asp Phe Asn Asn Gly Asp Ile Gln Lys Phe Ala Ile Glu Asn Asn Gly
115 120 125
Ser Ile Arg Val Lys Ile Asn Asn Gln Val Trp Asp Val Tyr Arg Gly
130 135 140
Glu Thr Trp Asp Asn Ala Pro Ile Val Gly Tyr His Tyr Gln Gly Ser
145 150 155 160
Gln Asn Gln Lys Phe Ser Leu Val Lys Asn Gly Val Val Ser Ile Thr
165 170 175
Arg Pro Gln Met Gly Thr Leu Pro Pro Ala Pro Asp Phe Lys Thr Asn
180 185 190
Asp Leu Asn Glu Val Leu Pro Glu Lys Thr Asp Pro Val Asn Thr Lys
195 200 205
Val Thr Phe Ile Pro Tyr Phe Met Ile Lys Asp Pro Ser Tyr Asn His
210 215 220
Gln Gln Gln Met Gln Leu Ser Pro Tyr Tyr Gln Leu Val Arg Arg Gln
225 230 235 240
Tyr Trp Glu Lys Lys Thr Gln Arg Ile Leu Gly Pro Ser Asp Lys Tyr
245 250 255
Glu Tyr Ser Gln Thr Val Gly Val Ser Arg Thr Asp Gln Thr Ser Met
260 265 270
Thr Lys Thr Thr Glu Ile Ser Ile Gly Leu Asp Leu Gly Phe Met Phe
275 280 285
Lys Gly Phe Ser Ala Gly Leu Ser Thr Ser Ile Thr Glu Thr Leu Ser
290 295 300
Val Thr Lys Ser Thr Ser Asn Thr Glu Ser Thr Ala Thr Thr Gln Ile
305 310 315 320
Val Thr Tyr Ser Asn Pro Phe Thr Tyr Ser Ile Ala Arg Ala Asn Tyr
325 330 335
Met Leu Ile Asp Glu Tyr Tyr Ile Thr Arg Ala Asn Gly Glu Lys Ile
340 345 350
Val Val Asp Asn Ala Thr Tyr Trp Arg Val Pro Asn Pro Glu Arg Thr
355 360 365
Val Ala Arg Ile Ile Pro Arg Asn
370 375
<210> 152
<211> 552
<212> БЕЛОК
<213> Bacillus sp.
<400> 152
Met Lys Tyr Lys Asn Arg Lys His Ala Lys Arg Lys Tyr Lys Gln Thr
1 5 10 15
Leu Leu Ala Thr Val Pro Thr Met Thr Ser Gly Val Asp Thr Leu Gly
20 25 30
Ser Thr Ser Ser Ala Phe Ala Glu Glu Lys Ala Thr Glu Ala Gln Gln
35 40 45
Ala Ala Met Asp Ser Leu Gly Val Gly Gly Gly Ala Ser Thr Asp Asp
50 55 60
Ala Phe Lys Ala Ala Gly Glu Ala Leu Lys Glu Val Ala Lys Leu Lys
65 70 75 80
Lys Asp Gly Lys Asn Lys Phe Thr Gln Glu Ala Gln Asp Thr Ile Thr
85 90 95
Leu Leu Gly Lys Ala Ala Pro Ile Met Tyr Ala Asp Ala His Ala Ser
100 105 110
Arg Glu Ser Phe Asn Asn Thr Leu Arg Thr Leu Ser Leu Gly Val Leu
115 120 125
Asp Tyr Phe Pro Tyr Gly Lys Ile Ala Ile Ser Pro Ile Leu Gly Leu
130 135 140
Leu Trp Pro Glu Ile Gly Asn Lys Asn Lys Asn Gln Phe Asp Ser Leu
145 150 155 160
Met Lys Asp Met Lys Ile Leu Val Lys Glu Gly Ile Glu Thr Tyr Asp
165 170 175
Leu Ser Gly Leu Lys Gln Asn Ile Ile Ser Leu Lys Thr Gln Met Gln
180 185 190
Thr Phe Glu Lys Leu Val Asn Ser Asn Thr Leu Thr Glu Gly Leu Tyr
195 200 205
Ser Ala Gly Ser Asp Glu Glu Ser Ala Arg Thr Ala Ala Gln Ile Leu
210 215 220
Gln Asp Thr Phe His Asn Leu Ile Asn Asp Cys Gln Lys Asp Asn Leu
225 230 235 240
Asp Thr Ala Glu Leu Pro Lys Tyr Thr Thr Ile Ala Thr Ile His Leu
245 250 255
Leu Phe Leu Asn Tyr Met Glu Lys Asn Trp Ser Ser Pro Gln Leu Gln
260 265 270
Tyr Pro Lys Ser Thr Phe Asp Lys Ile Phe Ala Asp Asp Ile Lys Asn
275 280 285
Ala Ala Gln Thr Tyr Met Asp Tyr Ile Gln Lys Thr Tyr Thr Ser Gly
290 295 300
Asn Ser Gln Phe Val Ser Lys Leu Lys Asp Val Val Gly Tyr Glu Ser
305 310 315 320
Ser Phe Pro Gln Gln Thr Gln Arg Asp Leu Phe Lys Lys Tyr Ser Asp
325 330 335
Leu Ser Lys Thr Val Ala Ala Met Glu Ser Gly Lys Asp Asn Thr His
340 345 350
Thr Pro Ala Val Ser Leu His Thr Met Lys Glu Arg Leu Asn Glu Ala
355 360 365
Glu Lys Asn Tyr Asn Asp Tyr Lys Asn Leu Val Thr Ile Gln Asp Asn
370 375 380
Tyr Tyr Asn Asn Thr Ile Gly Asn Lys Ala Phe Asp Gly Ile Leu Arg
385 390 395 400
Ala Val Leu Gly Thr Trp Lys Lys Ala Glu Asn Gly Lys Trp Ile Phe
405 410 415
Val Asp Ser Ala Gly His Lys Lys Ile Asp Trp Leu His Phe Thr Thr
420 425 430
Thr Tyr Phe Tyr Leu Ala Pro Ser Gly Gly Thr Glu Asn Ser Asp His
435 440 445
Val Lys Phe Thr Glu Gly Glu Met Val Thr Asp Trp Val Lys Ile Arg
450 455 460
Ala Gln Trp Tyr Tyr Phe Ser Pro Ser Glu Gly Thr Lys Asn Ser Gly
465 470 475 480
Gln Lys Lys Phe Ala Glu Gly Glu Met Met Thr Gly Trp Val Glu Ile
485 490 495
Ala Asp Lys Lys Pro Asn Asp Arg Pro Gly Glu Arg Thr Gly Thr Lys
500 505 510
His Trp Tyr Tyr Leu Asn Pro Glu Asn Gly Ser Met Ile Gln Asp Lys
515 520 525
Lys Glu Val Gln Ile Asp Glu Lys Lys Tyr Asp Phe Asp Ser Asn Gly
530 535 540
Val Cys Ile Thr Pro Lys Gly Tyr
545 550
<210> 153
<211> 386
<212> БЕЛОК
<213> Bacillus sp.
<400> 153
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Thr Pro Glu
20 25 30
Lys Asn Gly Tyr Tyr Ser Ile His Leu Ala Ser Asn Thr Trp Lys Lys
35 40 45
Trp Asp Val Ala Val Ala Ser Leu Asp Asn Gly Ala Gly Ile Leu Leu
50 55 60
Tyr Asp Val Ser Gly Lys Ile Pro Asp Asn Gln Gln Phe Ala Phe Phe
65 70 75 80
Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys
85 90 95
Pro Val Thr Ile Gly Asp Gly Val Trp Pro Met Asp Gly Leu Gly Gly
100 105 110
Asn Ser Gly Ala Leu Lys Gln Asn Asn Trp Thr Gly Asn Ala Asn Glu
115 120 125
Lys Trp Tyr Leu Arg Asp Leu Gly Gly Asn Asp Phe Glu Ile Val Asn
130 135 140
Gln Gly Thr Gly Lys Val Ala Ser Tyr Ala Trp Val Gly Asp Leu Val
145 150 155 160
Arg His Phe Glu Tyr Val Asp Leu Asp Asn Ala Asn Pro Ser Asp Arg
165 170 175
Asp Arg Val Phe His Ile Pro Ala Ala Arg Ser Thr Leu Thr Val Pro
180 185 190
Thr Leu Pro Ala Thr Gly Thr Arg Pro Val Ala Pro Asn Tyr Thr Gly
195 200 205
Gly Ile Asp Glu Gln Leu Pro Gln Thr Ser Lys Ser Val Ile Val Gly
210 215 220
Ala Ser Leu Ile Pro Cys Ile Met Val Lys Asp Ser Gln Thr Asn Asp
225 230 235 240
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu
245 250 255
Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu
260 265 270
Thr Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met
275 280 285
Thr Asp Thr Leu Ser Met Lys Phe Gly Ala Asp Phe Gly Ile Lys Phe
290 295 300
Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln
305 310 315 320
Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser
325 330 335
Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr
340 345 350
Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val
355 360 365
Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys
370 375 380
Thr Leu
385
<210> 154
<211> 373
<212> БЕЛОК
<213> Bacillus sp.
<400> 154
Val Gly Lys Ser Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Ser Gly Asn Gln Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Glu Asn Ala Pro Ser
35 40 45
Glu Arg Phe Phe Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Ile Ser Gly Asn Gln Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Phe Glu Trp Leu Gly Asp Ala Pro Ser
85 90 95
Glu Tyr Trp Tyr Phe His Arg Glu Ala Asp Gly His Tyr Val Ile Glu
100 105 110
Ser Lys Leu Ser Gly Lys Val Leu Asp Ile Ala Gly Asn Ser Thr Ala
115 120 125
Asn Asn Ala Asn Val Gln Gln Phe Glu Phe Leu Ala Asp Ala Pro Ser
130 135 140
Glu Arg Phe Ala Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Asn Thr Gln Pro Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Glu Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val
180 185 190
Pro Ala Ile Ser Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr
225 230 235 240
Thr Ser Lys Thr Gly Ile Lys Val Thr Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Trp Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly
260 265 270
Phe Ser Val Gly Met Ser Ser Gln Tyr Ser Thr Gln Leu Gln Thr Ser
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu
290 295 300
Ile Lys Asn Pro Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Ser Val Gln Arg Lys Ser Gly Thr Ile Val Asn Ser
325 330 335
Pro Trp Thr Met Thr Asp Lys Thr Arg Thr His Ala Val Thr Tyr Pro
340 345 350
Asn Ala Glu Glu Lys Ala Leu Asn Glu Asn Thr Lys Gln Leu Ser Lys
355 360 365
Ala Glu Ser Val Asn
370
<210> 155
<211> 373
<212> БЕЛОК
<213> Bacillus sp.
<400> 155
Val Gly Lys Ser Met Ile Phe Lys Val Gly Met Lys Tyr Arg Phe Arg
1 5 10 15
Asn Lys Asn Ser Gly Lys Tyr Leu Asp Ile Ala Gly Asn Gln Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Leu Val Lys Asp Leu Pro Ser
35 40 45
Glu Gln Phe Phe Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Ile Ile
50 55 60
Asn Val Asn Ser Gly Lys Val Leu Asp Ile Ala Gly Asn Gln Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Phe Glu Trp Arg Gly Asp Leu Pro Ser
85 90 95
Glu Tyr Trp Tyr Phe His Leu Glu Glu Asp Gly Tyr Tyr Val Ile Glu
100 105 110
Ser Lys His Ser Gly Lys Val Leu Asp Ile Ala Gly Asn Gln Thr Gly
115 120 125
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Ile Lys Asn Leu Pro Ser
130 135 140
Glu Arg Phe Ala Ala Glu Glu Val Glu Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Ser Thr Gln Pro Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Lys Glu Thr Glu His Val Val Thr Ala Phe Thr Ile Val
180 185 190
Pro Ala Ile Ser Val Arg Asp Pro Leu Tyr Gly Gly Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr
225 230 235 240
Thr Ser Lys Thr Gly Met Lys Val Thr Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Leu Ser Ile Gly Val Asp Met Gly Phe Asn Phe Lys Gly
260 265 270
Val Ser Val Gly Met Ser Thr Gln Tyr Ser Thr Gln Leu Gln Ala Ser
275 280 285
Thr Ser His Thr Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu
290 295 300
Ile Lys Asn Pro Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Ser Val Glu Arg Lys Asn Gly Thr Ile Val Asn Ser
325 330 335
Pro Trp Thr Met Thr Asp Lys Thr Ser Ala His Ser Val Thr Tyr Pro
340 345 350
Asn Ala Glu Glu Lys Ala Leu Asn Glu Asn Thr Lys Gln Leu Ser Lys
355 360 365
Val Glu Ser Val Asn
370
<210> 156
<211> 287
<212> БЕЛОК
<213> Bacillus sp.
<400> 156
Met Asn His Leu Ala Phe Ser Pro Arg Glu Leu Gly Gly Lys Asp Ser
1 5 10 15
Pro Phe Leu Leu Thr Ile Glu Glu Trp Arg Lys Phe Ala Lys Tyr Leu
20 25 30
Asn Asp Trp Met Ser Glu Pro Thr Ser Ile Asp Gln Val Lys Glu Ala
35 40 45
Ile Tyr Arg Ile Arg Asp Gly Ile Phe Lys Gln Asn Trp Asp Arg Leu
50 55 60
Ile Thr Thr Ser Ala Phe Arg Arg Leu Val Glu Glu Ser Ile Ala Thr
65 70 75 80
Lys Leu Ile Ile Arg Glu Lys Cys Arg Phe Trp Asp Asn Gly Gly Cys
85 90 95
Lys Asp Ile Leu Ile Leu Ser Gln Asn Ile Val Ala Phe Ala Asp Met
100 105 110
Ile Thr Ile Lys Tyr Tyr Asn Asp Leu Asn Gln Ile Ile Leu Glu Ala
115 120 125
Gln Asp Gly Glu Leu Phe Pro Asn Thr Lys Ala Lys Phe Ile Asn Ile
130 135 140
Cys Lys Asp Leu Ser Ser His Ala Gln Thr Tyr Tyr Gln Gln Ser Gln
145 150 155 160
Ser Met Glu Thr Ser Leu Ser Glu Phe Tyr Ser Glu Ile Gln Lys His
165 170 175
Glu Asp Thr Val Gln Asn Trp Ser Tyr Leu Arg Ser Gln Leu Ala Leu
180 185 190
His Thr Ser Asn Asp Phe Ala Val Ala Gln His Thr Asn Val Tyr Leu
195 200 205
Val Val Pro Val Glu His Ser Val Arg Tyr Lys Glu Asn Val Leu Pro
210 215 220
Val Ile Arg Lys Val His Arg Ile Trp Gly Ser Ile Ser Tyr Asp Leu
225 230 235 240
Lys Glu Leu Ala Asp Asn Ile Glu Asp Ile Glu Asn Leu Glu Ala Phe
245 250 255
Ile Ala Ala Leu Glu Leu Glu Leu Ala Phe Glu Asn Trp Ser Ala Ile
260 265 270
Arg Glu Glu Ala Glu Asp Phe His Lys Asn Ala Glu Asn Phe Ser
275 280 285
<210> 157
<211> 397
<212> БЕЛОК
<213> Bacillus sp.
<400> 157
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ser Ser Glu Ala Ala Ser Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Ser Val His Leu Lys Ala Asn Arg Asp Lys Val
35 40 45
Trp Asp Val Tyr Arg Asn Asn Thr Tyr Asp Asn Ala Gly Ile Leu Leu
50 55 60
Tyr Gly Gln Asn Gly Gln Asp Asn Gln Lys Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Gly Asp Pro Ile Ala Asn Asp Asp Gly Val Arg Arg Met Thr
100 105 110
Leu Phe Asp Asn Glu Gly Leu Arg Gln Glu Ser Trp Thr Gly Ala Thr
115 120 125
Asp Gln Gln Trp Tyr Leu Arg Asp Gln Gly Gly Ser Asn Tyr Glu Ile
130 135 140
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Trp Glu Gly Thr
145 150 155 160
Leu Ala Gln Asn Trp Glu Tyr Thr Asp Leu Asp Glu Ser Asn Pro Ser
165 170 175
Asp Ser Asp Arg Val Phe Tyr Ile Ser Ala Gly Pro Val Asn Gln Gly
180 185 190
Thr Gln Pro Leu Tyr Gly Thr Phe Ser Leu Pro Lys Leu Pro Ala Val
195 200 205
Gly Asn Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Ile Glu Gln Gln
210 215 220
Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro
225 230 235 240
Cys Phe Met Val Asn Asp Gly Gln Ala Asp Asn Tyr Thr Lys Ile His
245 250 255
Asn Ser Pro Tyr Tyr Val Leu Gln Lys Glu Glu Tyr Trp Asp Lys Thr
260 265 270
Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu Ser Tyr Ser Tyr Lys
275 280 285
Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser
290 295 300
Met Lys Ile Gly Ala Asp Leu Gly Leu Lys Phe Gly Gln Gln Thr Ala
305 310 315 320
Ser Ile Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Val Ser Thr
325 330 335
Thr Asn Thr Glu Ala Thr Glu Glu Thr Arg Ala Tyr Thr Ala Val Gly
340 345 350
Glu Ala Gly Arg Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys
355 360 365
Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val Ser Asp Pro Trp Val
370 375 380
Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys Thr Ser
385 390 395
<210> 158
<211> 360
<212> БЕЛОК
<213> Bacillus sp.
<400> 158
Met Glu Ile Lys Lys Asn Glu Phe Tyr Lys Leu Val Asn Lys Asn Ser
1 5 10 15
Glu Lys Val Ala Asp Val Ser Trp Arg Ser Thr Asp Asp Ser Ala Thr
20 25 30
Leu Glu Gln Leu Thr Trp Asp Gly Glu Asp Phe Gln Lys Trp Leu Leu
35 40 45
Ile Glu Leu Asp Gly Gly Tyr Tyr Ala Leu Val Asn Lys Asn Ser Gly
50 55 60
Lys Val Ala Asp Val Ser Trp Arg Ser Thr Asp Asp Ser Ala Thr Leu
65 70 75 80
Glu Gln Leu Thr Trp Asp Gly Glu Asp Phe Gln Gln Trp Ile Val Lys
85 90 95
Asp Ala Glu Glu Gly Tyr Val Ser Leu Trp Asn Lys Asn Ser Gly Lys
100 105 110
Val Ala Asp Val Ser Trp Arg Ser Lys Asp Asp Ser Ala Thr Leu Glu
115 120 125
Gln Leu Thr Trp Asn Asp Glu Asp Phe Gln Lys Trp Ser Leu Val Glu
130 135 140
Val Glu Ser Phe Asp Leu Pro Ser Val Glu Val Ser Thr Leu Pro Pro
145 150 155 160
Val Pro Gln Tyr Thr Ser Leu Asn Asp Asn Leu Pro Asp Val Thr Asn
165 170 175
Ser Val Val Thr Ala Tyr Thr Leu Met Pro Cys Ile Met Val Asn Asp
180 185 190
Ser Trp Asn Ser Met Asp Lys Met Lys Asn Ser Pro Tyr Tyr Ile Leu
195 200 205
Gln Lys Ser Gln Tyr Trp Lys Lys Leu Asp Ser His Thr Phe Ala Pro
210 215 220
Asn Ile Ser Tyr Thr Ser Asp Thr Lys Tyr Gly Met Thr Asn Thr Asp
225 230 235 240
Gln Glu Ser Met Thr Asn Thr Thr Gly Ile Ser Val Thr Val Asp Ala
245 250 255
Gly Phe Ser Phe Lys Gln Leu Ser Ser Ser Ile Ser Lys Thr Val Thr
260 265 270
Asp Glu Leu Gln Val Ser Ile Ser Thr Ala Thr Glu Leu Met Thr Glu
275 280 285
Gln Glu Asn Ile Glu Thr Ile Glu Asn Thr Asn Asp Tyr Glu Ile Ala
290 295 300
Trp Ser Leu Tyr Ala Leu Val Thr Glu Tyr Glu Leu Lys His Ala Asp
305 310 315 320
Gly Thr Ser Val Thr Gln Ser Trp Thr Val Ile Asn Lys Asn Asp Glu
325 330 335
Arg Glu Ser Tyr Tyr Pro Leu Ser Ser Lys Leu Gln Lys Thr Ser Leu
340 345 350
Glu Asp Gly Ser Leu Gln Asn Lys
355 360
<210> 159
<211> 239
<212> БЕЛОК
<213> Serrati asp.
<400> 159
Met Leu Asp Lys Lys Lys Ala Ser Ile Ser Ser Pro Val Ile Thr Lys
1 5 10 15
Asn Leu Phe Ile Arg Leu Val Ala Asp Asp Asn Asn Phe Asp Asn Ile
20 25 30
Ile Ser Ile Thr Asp Pro Ser Val Leu Ile Leu Ala Leu Lys Thr Ala
35 40 45
Ala Leu Phe Gln Gly Ala Leu Val Pro Ala Glu Asn Asn Ser Leu Arg
50 55 60
Phe Asp Val Gln Lys Ala Met Glu Leu Val Asn Gln Asn Pro Glu Leu
65 70 75 80
Ser Val Thr Asn Tyr Thr Asp Ile Lys Lys Glu Thr Ser Asp Ser Arg
85 90 95
Thr Ser Val Met Thr Asp Met Val Leu Ser Thr Ile Lys Thr Val Leu
100 105 110
Gly Ile Val Leu Ala Pro Ala Pro Ala Gln Gln Leu Gln Ser Ala Ile
115 120 125
Glu Gly Ala Tyr Val Gly Leu Asp Glu Thr Lys Asp Ser Ala Trp Ile
130 135 140
Phe Trp Glu Lys Lys Lys Asp Thr Lys Thr Thr Tyr Gln Tyr Asn Ile
145 150 155 160
Thr Phe Ser Val Glu Ala Gly Pro Thr Gly Leu Val Leu Leu Thr Cys
165 170 175
Pro Met Gly Leu Thr Ile Glu Val Asn Lys Glu Tyr Glu Arg Val Leu
180 185 190
Phe Ile Thr Leu Thr Asp Arg Glu Ser Tyr Ser Cys Arg Ile Gln Ala
195 200 205
Leu Thr Thr Ala Gln Leu Ala Lys Pro Ala Ala Lys Asn Ala Glu Ala
210 215 220
Ala Glu Val Leu Ser Val Leu Ile Gly Asp Gly Pro Arg Arg Val
225 230 235
<210> 160
<211> 553
<212> БЕЛОК
<213> Bacillus sp.
<400> 160
Met Asn Tyr Lys Asn Arg Thr Asn Ala Lys Gln Lys Tyr Lys Gln Ser
1 5 10 15
Leu Phe Leu Thr Ile Ala Thr Met Thr Leu Gly Gly Ser Ile Leu Gly
20 25 30
Asn Thr Ala Ser Ala Phe Ala Ala Glu Asn Thr Thr Glu Lys Gln Lys
35 40 45
Val Ser Ser Tyr Pro Lys Asn Ser Asn Thr Val Gly Ser Asn Val Phe
50 55 60
Lys Val Ala Ala Asp Gly Ser Val Thr Leu Leu Asn Ser Thr Pro Phe
65 70 75 80
Asp Ser Pro Thr Val Lys Ala Ile Thr Leu Leu Gly Gly Asn Leu Leu
85 90 95
Asn Gln Ala Tyr Leu Asp Ala Tyr Asn Lys Asp Phe Thr Gly Thr Ala
100 105 110
Arg Thr Leu Val Leu Gly Ala Thr Ala Leu Ile Pro Tyr Val Gly Ser
115 120 125
Phe Ile Ser Pro Ile Ile Gly Leu Leu Trp Pro Ala Asn Val Gly Thr
130 135 140
Asp Met Thr Ala Gln Thr Lys Ala Leu Val Ala Leu Ile Asn Glu Gln
145 150 155 160
Ile Ala Asp Tyr Asp Ile Glu Thr Leu Glu Gln Lys Gly Lys Thr Leu
165 170 175
Lys Asp Leu Met Ser Arg Phe Asp Asp Leu Leu Asn Gly Lys Pro Val
180 185 190
Thr Phe Gly Glu Gln Gly Thr Val Gln Glu Thr Leu Arg Asn Lys Ala
195 200 205
Ser Asn Ile Asn Asp Lys Phe Ile Glu Val Ile Asn Asp Cys Gln Lys
210 215 220
Arg Ser Phe Lys Ala Glu Glu Leu Pro Ile Tyr Thr Leu Ile Ala Thr
225 230 235 240
Ala His Leu Leu Phe Leu Asn Met Ile Glu Gln Asn Gly Gln Ser Ser
245 250 255
Arg Ile Gln Met Asp Asp Glu Thr Tyr Lys Gly Tyr Val Glu Asp Phe
260 265 270
Lys Arg Leu Pro Ile Glu Tyr Ala Asn Tyr Ile Glu Ala Thr Ser Lys
275 280 285
Leu Gly Lys Gln Lys Phe Asn Lys Lys Met Asp Asp Thr Ile His Ala
290 295 300
Ala Lys Lys Gln Ala Leu Val Gly Ile Ile Ser Ser Asp Glu Asn Thr
305 310 315 320
Asn Leu Glu Asn Leu Glu Lys Thr Arg Lys Gln Cys Leu Asp Glu Ala
325 330 335
Lys Leu Leu Gln Gln Ile Gly Glu Val Tyr Gly Ala Glu Lys Ala Arg
340 345 350
Gln Lys Ala Gln Lys Ala Tyr Glu Tyr Lys Lys Gln Tyr Glu Asp Leu
355 360 365
Ile Gln Lys Arg Gly Asn Tyr Ile Gln Asn Thr Ala Asp Asn Glu Ala
370 375 380
Phe Lys Leu Ala Lys Lys Ser Met Glu Glu Leu Met Ile Asp Val Arg
385 390 395 400
Ile Pro Asn Gly Lys Tyr Ile Ile Gln Ser Thr Gln Asn Ser Ser Met
405 410 415
Val Val Gly Met Ser Gly Asn Asn Ile Gln Leu Glu Glu Asn Asn Gly
420 425 430
Gln Leu Asn Asn Lys Phe Phe Ile Ile Tyr Asp Gln Ala Arg Asn Ser
435 440 445
Tyr Val Ile Glu Thr Ser Ile Glu Thr Gly Leu Ser Ser Tyr Met Ala
450 455 460
Ala Asn Ile Asp Tyr Ser Asn Val Asn Lys Ser Gly Val Asn Val Tyr
465 470 475 480
Ala Ile Leu Ser Thr Ser Ser His Glu Tyr Pro Asp Ile Leu His Trp
485 490 495
Lys Leu Lys Arg Glu Arg Gly Ser Lys Phe Val Tyr Leu Glu Asn Leu
500 505 510
Ala Asn Gly Gln Val Leu Asp Val Ser Asn Ala Gly Thr Glu Lys Gly
515 520 525
Thr Asn Ile Ile Thr Trp Pro Leu Lys Gln Gln Gly Gln Asn Gln Asn
530 535 540
Gln Gly Phe Glu Leu Val Pro Thr Asn
545 550
<210> 161
<211> 398
<212> БЕЛОК
<213> Bacillus sp.
<400> 161
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ala Ser Glu Ala Ala Ser Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Ser Ile His Leu Glu Ser Asn Lys Asn Lys Val
35 40 45
Trp Asp Val Phe Asn Asn Ala Thr Tyr Asp Asn Ala Gly Ile Leu Leu
50 55 60
Tyr Gly Gln Thr Asn Ser Asp Asn Gln Lys Phe Ala Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Gly Asp Pro Ile Ile Ile Asp Asp Gly Val Arg Arg Met Thr
100 105 110
Ala Phe Asn Asn Glu Gly Leu Arg Gln Thr Ser Trp Thr Gly Ala Ser
115 120 125
Ala Glu Lys Trp Tyr Leu Arg Glu Lys Ser Pro Ser Asn Tyr Glu Ile
130 135 140
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Trp Gln Gly Thr
145 150 155 160
Leu Ala Gln Asn Trp Glu Tyr Ala Asp Leu Asp Asn Ser Asn Pro Thr
165 170 175
Asp Gly Asp Arg Val Phe Tyr Val Ser Ala Gly Gln Val Asn Gln Gly
180 185 190
Asn Val Pro Phe His Gly Thr Phe Ala Ile Pro Gln Leu Pro Ala Thr
195 200 205
Gly Asn Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Val Asp Gln Gln
210 215 220
Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro
225 230 235 240
Cys Phe Met Val Asn Asp Asn Gln Ala Ser Asp Tyr Thr Lys Ile His
245 250 255
Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr
260 265 270
Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg Asn Tyr Ser Phe Lys
275 280 285
Thr Gly Met Thr Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser
290 295 300
Met Lys Ile Gly Ala Asp Leu Gly Leu Lys Phe Gly Glu Gln Ser Ala
305 310 315 320
Ser Ile Lys Ser Glu Val Ser Arg Thr Leu Gln Thr Glu Ile Ser Thr
325 330 335
Thr Asn Thr Glu Ala Thr Glu Glu Thr Val Thr Ser Thr Val Glu Ser
340 345 350
Glu Ala Gly Lys Thr Thr Gly Phe Thr Glu Tyr Gln Leu Ala Thr Lys
355 360 365
Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val Ser Asp Pro Trp Val
370 375 380
Val Lys Asn Asn Lys Ile Thr Ile Ala Arg Lys Asn Ala Gln
385 390 395
<210> 162
<211> 573
<212> БЕЛОК
<213> Bacillus sp.
<400> 162
Met Lys Tyr Lys Asp Arg Lys Ser Ala Lys Arg Lys Tyr Lys Gln Ala
1 5 10 15
Leu Leu Ala Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Ser Thr Ala Ser Val Phe Ala Ala Glu Asp Thr Gly Asn Val Ser Phe
35 40 45
Leu His Pro Lys Lys Glu Pro Ala Lys Lys Glu Asp Pro Met Asp Ile
50 55 60
Leu Asn Trp Asn Lys Tyr Glu Asp Val Phe Asn Lys Asn Arg Gly Asn
65 70 75 80
Ser Ile Ala Lys Leu Thr Gly Ala Gly Leu Lys Glu Ile Phe Ser Gln
85 90 95
Gly Asn Lys Ser Ala Pro Asp Phe Asn Gln Leu Ala Lys Thr Leu Thr
100 105 110
Met Thr Ala Ile Asp Leu Phe Pro Gly Gly Met Phe Ile Thr Pro Leu
115 120 125
Leu Asn Leu Phe Trp Pro Ser Ala Gln Gly Ser Asn Gly Asn Glu Thr
130 135 140
Leu Ile Lys Asn Leu Thr Glu Lys Leu Thr Lys Val Met Asp Glu Lys
145 150 155 160
Ile Asp Ala Glu His Leu Asn Gln Met Thr Asn Asp Phe Lys Ala Leu
165 170 175
Ile Glu Asp Ile Thr Arg Leu Gln Asn Phe Leu Asn Asn His Ser Lys
180 185 190
Phe Val Leu Pro Asp Met Ser Phe Thr Ala Phe Val Asp Val Asp Asp
195 200 205
Ser Gln Leu Tyr Ser Gly Thr Asn Leu Thr Ser Ala Gly Ala Val Ala
210 215 220
Leu Asn Ile Glu Ser Ala Phe Thr Arg Ile Leu Thr Gln Val Ser Asp
225 230 235 240
Glu Lys His Asp Thr Thr Asp Leu Met Leu Tyr Thr Asn Val Ala Asn
245 250 255
Ile His Leu Gln Phe Leu Lys Tyr Ile Glu Lys Tyr Gly Ala Ser Glu
260 265 270
Lys Phe Gly Leu Ser Lys Leu Asp Leu Glu Lys Phe Phe Lys Ser Asp
275 280 285
Thr Ile Ser Ser Ile Thr Glu Lys Tyr Ala Lys His Ile Glu Asp Thr
290 295 300
His Ser Lys Ala Met Asp Lys Leu Asn Lys Arg Ile Glu Asp Leu Gly
305 310 315 320
Gly Asn Glu Glu Thr Leu Asn Asn Lys Ile Lys Glu Val Glu Asn Ser
325 330 335
Arg Ala Gln Arg Ser Ile Asp Pro Asp Ala Ala Ile Leu Gln Glu Lys
340 345 350
Glu His Glu Lys Trp Lys Lys Glu Glu Val Thr Lys Leu Lys Ala Asn
355 360 365
Ile Glu Lys Val Lys Glu Ile Glu Thr Glu Lys Gln Asn Leu Thr Glu
370 375 380
Leu Thr Ile Lys Asn Glu Gly Phe Lys Ala Ala Ser Gly Lys Thr Leu
385 390 395 400
Asn Val Leu Glu Ser Gly Phe Phe Lys Gln Asn Gly Lys Thr Tyr Tyr
405 410 415
Arg Gly Tyr Arg Gly Asp Glu Thr Lys Val Glu Asn Gly Thr Leu Ala
420 425 430
Thr Gly Trp Gln Thr Ile Lys Gly Lys Thr Tyr Tyr Phe Gly Ala Pro
435 440 445
Gly Asp Asn Thr Lys Val Glu Asn Asn Thr Ala Ala Thr Gly Trp Gln
450 455 460
Thr Ile Lys Gly Lys Thr Tyr Tyr Phe Gly Ala Pro Gly Asp Asn Thr
465 470 475 480
Lys Val Glu Asn Asn Thr Ala Ala Thr Gly Leu Gln Lys Ile Gly Gly
485 490 495
Lys Thr Tyr Tyr Phe Gly Lys Pro Gly Asp Gly Thr Thr Val Asp Asn
500 505 510
Tyr Thr Met Ala Thr Gly Trp Val Val Leu Asp Asn Gly His Asp Trp
515 520 525
Tyr Tyr Cys Ala Pro Ser Asp Leu Thr Thr Asp Asp Gly Lys His Phe
530 535 540
Ser Lys Gly Glu Leu Leu Thr Asn Thr Glu Phe Thr Ile Asn Gly Lys
545 550 555 560
Thr His Val Phe Pro Glu Ser Gly Arg Ala Tyr Ala Lys
565 570
<210> 163
<211> 566
<212> БЕЛОК
<213> Bacillus sp.
<400> 163
Met Lys Tyr Lys Asp Cys Ile Gln Ala Lys Arg Asn Tyr Lys Gln Thr
1 5 10 15
Ile Phe Ala Ala Ala Thr Thr Leu Thr Leu Gly Val Ser Ala Leu Ala
20 25 30
Gly Pro Ala Ser Ala Phe Ala Ala Glu Asn Thr Lys Asp Thr Gln Lys
35 40 45
Gln Ser Gln Asn Val Val Ser Lys Val Met Ser Asn Thr Gly Asp Lys
50 55 60
Ala Phe Glu Ile Leu Asn Asp Gly Thr Ile Lys Val Ile Asn Glu Gln
65 70 75 80
Tyr Phe Pro Lys Gly Asn Glu Glu Trp Phe Lys Lys Thr Leu Val Lys
85 90 95
Met Gly Gly Gln Thr Phe Leu Asp Thr Met Lys Ser Leu Asn Gly Asn
100 105 110
Gly Ala Met Ser Ile Asn Asn Phe Ala Lys Asp Met Val Val Val Ser
115 120 125
Thr Ser Leu Ile Pro Tyr Ala Gly Ala Ile Ile Ser Pro Leu Ile Asn
130 135 140
Leu Leu Trp Pro Ser Ser Val Ser Asp Ser Gln Asn Gln Ile Gln Leu
145 150 155 160
Leu Met Lys Met Met Asp Asn Lys Ile Ser Asp Tyr Asp Ile Asn Ser
165 170 175
Leu Lys Thr Asp Val Lys Ala Ala Val Glu Glu Leu Asn Lys Leu Glu
180 185 190
Val Gly Leu Gln Gly Ala Asn Gly Phe Lys Ala Tyr Arg Thr Ser Ala
195 200 205
Asp Tyr Asn Ala Gly Gln Ala Glu Ala Val Asn Arg Ala Phe Arg Lys
210 215 220
Ile Met Gln Asn Ser Leu Lys Glu Gly Leu Glu Lys Ser Glu Leu Pro
225 230 235 240
Ile Tyr Thr Met Val Ala Thr Ala Lys Leu Ile Phe Leu Ser Tyr Met
245 250 255
Val Glu Asn Gly Gly Asn Ala Asp Phe Lys Tyr Asp Asp Ala Thr Leu
260 265 270
Lys Asn Ala Tyr Ile Asn Ser Asp Leu Ala Val Asp Ile Asn Asn Phe
275 280 285
Glu Glu His Ile Gln Lys Ile Ser Ala Glu Ser Val Glu Arg Leu Asn
290 295 300
Thr Leu Asn Thr Glu Val Pro Lys Ala Val Asp Gln Met Ser Ala Leu
305 310 315 320
Gln Thr Lys Ile Asn Lys Leu Gln Asp Ala Ala Asn Lys Gly Asn Pro
325 330 335
Glu Ala Ala Ala Ala Leu Ala Gly Ala Val Glu Leu Leu Asn Lys Leu
340 345 350
His Thr Gln Tyr Asp Pro Met Ile Lys Glu Gln Lys Lys Leu Gln Asp
355 360 365
Tyr Val Ser Ser Thr Ala Lys Asn Asp Ala Phe Lys Val Ala Leu Glu
370 375 380
Thr Ala Lys Asn Thr Tyr Lys Lys His Gly Gly Gln Ile Lys Glu Lys
385 390 395 400
Asp Gly Phe His Gln Glu Gly Asn Asn Trp Tyr Tyr Thr Lys Asn Gly
405 410 415
Glu Lys Gln Lys Gly Trp Ile Lys Asp Asn Gly Lys Asp Tyr Tyr Leu
420 425 430
Ser Lys Glu Asp Gly His Met Lys Thr Gly Trp Val Gln Phe Glu Lys
435 440 445
Asp Asn Glu Arg Asp Tyr Asn Pro Ala Ala Val Asn Gln Asp Asn Val
450 455 460
Thr Thr Tyr Tyr Leu Asp Thr Asp Gly His Met Val Thr Gly Trp Lys
465 470 475 480
Asn Ile Gly Gly Tyr Tyr Tyr Tyr Phe Asn Lys Pro Glu Asn Thr Gly
485 490 495
Glu Asn Gly His Tyr Phe Lys Asn Gly Glu Met Ile Lys Gly Trp Ile
500 505 510
Arg Leu Ser Glu Asp Gly Ser Glu Lys Glu Val Ala Asp Gly Gly Tyr
515 520 525
Trp Tyr Tyr Leu Gly Arg Asp Gly Ser Met Leu Lys Asp Gly Thr Tyr
530 535 540
Leu Thr Phe Asp Arg Ala Gly Thr Ser Lys Gln Ala His Phe Asp Lys
545 550 555 560
Tyr Gly Arg Tyr Thr Gly
565
<210> 164
<211> 287
<212> БЕЛОК
<213> Bacillus sp.
<400> 164
Met Asn His Leu Ala Phe Ser Pro Arg Glu Leu Gly Gly Lys Asp Ser
1 5 10 15
Pro Phe Leu Leu Thr Ile Glu Glu Trp His Lys Phe Ala Lys Tyr Leu
20 25 30
Asn Asp Trp Met Ser Glu Pro Thr Ser Ile Asp Gln Val Lys Glu Ala
35 40 45
Ile Tyr His Ile Arg Asp Gly Ile Phe Lys Gln Asn Trp Asp Arg Leu
50 55 60
Ile Thr Thr Ser Ala Phe Arg Arg Leu Val Glu Glu Ser Ile Ala Thr
65 70 75 80
Lys Leu Ile Ile Arg Glu Lys Cys Arg Phe Trp Asp Asn Gly Gly Arg
85 90 95
Lys Asp Ile Leu Ile Leu Ser Gln Asn Ile Val Ala Phe Ala Asp Met
100 105 110
Ile Thr Ile Lys Tyr Tyr Asn Asp Leu Asn Gln Ile Asn Leu Glu Ala
115 120 125
Gln Asp Gly Lys Leu Ser Pro Asn Thr Lys Ala Lys Phe Ile Asn Ile
130 135 140
Cys Lys Asp Leu Ser Ser His Ala Gln Thr Tyr Tyr Gln Gln Ser Gln
145 150 155 160
Ser Met Glu Thr Ser Leu Ser Gln Leu Tyr Ser Glu Ile Gln Lys His
165 170 175
Glu Asp Thr Val Gln Asn Trp Ser Tyr Leu Arg Ser Gln Leu Ala Leu
180 185 190
Arg Thr Ser Asp Asp Leu Ala Val Ala Gln His Thr Asn Leu Tyr Leu
195 200 205
Val Ile Pro Val Glu His Ser Val Lys Tyr Lys Glu Asn Val Leu Pro
210 215 220
Val Ile Arg Lys Val His Arg Ile Trp Gly Ser Ile Ser Tyr Asp Leu
225 230 235 240
Lys Glu Leu Ala Asp Asn Ile Glu Asp Ile Lys Asn Leu Glu Ala Phe
245 250 255
Val Ala Ala Leu Glu Leu Glu Leu Ala Phe Glu Asp Trp Lys Ala Ile
260 265 270
Arg Asp Glu Ala Glu Asn Phe Tyr Lys Asn Ala Glu Asn Phe Ser
275 280 285
<210> 165
<211> 475
<212> БЕЛОК
<213> Bacillus sp.
<400> 165
Met Pro Met Phe Ile Phe Leu Ile Ile Ser Tyr Leu Ser Glu Asp Leu
1 5 10 15
Glu Ser Gln Lys Tyr His Ile Cys Thr Lys Trp Gly Leu Asn Phe Tyr
20 25 30
Ile Ala Lys Val Ile Leu Phe Arg Asn Lys Ser Ile Ser Asn Val Gln
35 40 45
Tyr Lys Ile Ile Phe Cys Asn Leu Val Gly Ile Leu Pro Thr Lys Leu
50 55 60
Gly Gly Asn Ile Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu
65 70 75 80
Ala Val Gly Ala Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Glu Ala
85 90 95
Ala Ala Pro Glu Asn Asn Arg Tyr Tyr Ser Ile His Leu Lys Ala Asn
100 105 110
Pro Lys Leu Ala Trp Asp Val Tyr Lys Gly Trp Asp Ser Asp Asn Ala
115 120 125
Gly Ile Ile Leu Tyr Asn Gly Ser His Gly Asp Asn Glu Gln Phe Ala
130 135 140
Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser
145 150 155 160
Gly Lys Pro Val Gly Phe Gly Gly Gln Ala Tyr Tyr Trp Thr Asp Gly
165 170 175
Val Arg Asp Gly Phe Val Met Asn Arg Asp Ala Leu Gln Gln Lys Asn
180 185 190
Trp Thr Gly Ser Ala Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn
195 200 205
Asn Asn Tyr Glu Ile Val Asn Gln Gly Thr Gly Lys Val Val Ser Trp
210 215 220
Ala Gly Thr Pro Val Thr Gly Gly Tyr Leu Asp Tyr Val Asp Leu Asp
225 230 235 240
Glu Ser Asn Pro Ser Asp Asn Asn Lys Val Phe Asn Ile Ala Lys Asn
245 250 255
Gly Phe Gly Gly Ile Gly Val Ser Ile Pro Gly Phe Asp Phe Asp Tyr
260 265 270
Gly Thr Phe Ser Leu Pro Thr Leu Pro Ala Val Gly Thr Arg Pro Asn
275 280 285
Ala Pro Asp Tyr Asn Thr Ser Gly Ala Ile Asp Gln Gln Leu Pro Gln
290 295 300
Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro Ser Ile Met
305 310 315 320
Val Lys Asp Asn Gly Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro
325 330 335
Tyr Tyr Val Leu Val Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu
340 345 350
Ile Ile Pro Ala Gly Ala Thr Ser Lys Tyr Thr Val Lys Ser Gly Val
355 360 365
Ser Glu Ile Asp Gln Lys Lys Met Ser Asp Thr Leu Ala Met Ser Phe
370 375 380
Gly Ser Asp Leu Gly Leu Lys Phe Gly Glu Ser Ser Leu Ser Ile Lys
385 390 395 400
Ser Ser Val Ser Arg Thr Ile Gln Thr Glu Val Thr Lys Thr Ser Thr
405 410 415
Asn Ala Thr Glu Glu Thr Glu Glu Lys Ser Ile Pro Ser Ile Pro Gly
420 425 430
Lys Asn Thr Gly Phe Thr Ala Tyr Gln Leu Val Thr Lys Tyr Thr Leu
435 440 445
Lys Arg Thr Asp Gly Ser Gly Val Thr Asp Pro Ser Ile Val Arg Asp
450 455 460
Ser Lys Gln Thr Ile Ala Arg Thr Ile Ala Asn
465 470 475
<210> 166
<211> 1559
<212> БЕЛОК
<213> Bacillus sp.
<400> 166
Met Thr Thr Tyr Lys Gly Asp Phe Pro Asp Ile Pro Ala Leu Phe Asp
1 5 10 15
Asn Glu Glu Asp Ile Ala Lys Ile Glu Ala Ile Asn Lys Glu Leu Arg
20 25 30
Gln Lys Tyr Leu Asn Arg Ser Glu Leu Tyr Arg Cys Pro Lys Asp Ile
35 40 45
Glu Met Leu Asp Lys Asn Ile Phe Met Asp Thr Pro Val Asp Leu Asp
50 55 60
Phe Tyr Ala Tyr Leu Thr Ala Glu Asp Phe Gly Ile Asn Lys Val Ile
65 70 75 80
Phe Tyr Tyr Asp Thr Asp Ile Val Phe Glu Ser Ile Leu Lys Lys Ser
85 90 95
Thr Phe Gly Ile Asp His Gly Tyr Phe Leu Gly Ser Leu Tyr Glu Arg
100 105 110
Ala Pro Asp Trp Arg Lys Asp Glu Val Ile Gln Leu Lys Leu Ser Val
115 120 125
Pro Lys Gly Thr Lys Val Thr Arg Val Gly Ser Leu Asp Asp Pro Lys
130 135 140
Tyr Ile Leu Gln Arg Glu Arg Thr Phe Gln Tyr Thr Asp Lys Thr Ile
145 150 155 160
Leu Arg Asp Asn Lys Gly Lys Pro Tyr Leu Glu Ile Ser Ala Gln Leu
165 170 175
Ile Asp Arg Glu Val Leu Glu Glu Ala Thr Leu Arg Gln Glu Lys Val
180 185 190
Ile Glu Asn Glu Leu Lys Asp Leu Phe Gly Val Arg Phe Lys Pro Asp
195 200 205
Phe Ile Lys Leu Glu Pro Ile Gly Leu Asn Ala Gly Tyr Val Leu Thr
210 215 220
His Gly Leu Asp Val Ala Trp Glu Ala Leu Glu Asn Leu Glu Ala Gly
225 230 235 240
Glu Ile Leu Ser Asp Asn Leu Phe Asp Lys Glu Gly Val Ile Phe Thr
245 250 255
Asn Gly Trp Met Leu His Thr Glu Ile Phe Thr Lys Leu Thr Lys Gly
260 265 270
Trp Gly Leu Lys Glu Arg Arg Glu Tyr Phe Glu Lys His Phe Asn Ile
275 280 285
Asp Ala Ala Gly Thr Thr Val Lys Trp Pro Asp Glu Thr Cys Gln Ser
290 295 300
Asn Val Arg Phe Ser Asn Leu Leu Tyr Gly Arg Gly Val Gly Gln Arg
305 310 315 320
Glu Ile Ile Glu Asp Phe Ser Ala Val Leu Leu His Glu Phe Phe His
325 330 335
Tyr Leu Ile His Thr Thr Asn Ser Phe Lys Glu Arg Pro Met Phe Lys
340 345 350
Gly Glu Gly Arg Ala Asp Phe Gly Gly Thr Ile Met Lys Leu Lys Leu
355 360 365
Leu Glu Gly Asp Asp Leu Val Lys Leu Leu Asp Asp Asp Tyr Ala Lys
370 375 380
Lys Ser Ser Glu Glu Phe Ile Cys Glu Ala Phe Arg Ala Lys Phe Asp
385 390 395 400
Ser Asp Val Val Ile Lys Ser Asn Phe Arg Lys Lys Val Ile Arg Thr
405 410 415
Asn Arg Phe Leu Asp Asn Leu Phe Asp Phe Glu Ala Pro Ser Thr Pro
420 425 430
Glu Asn Leu Arg Glu Leu Glu Val Gly Gly Glu Ser Val Lys Ile Ala
435 440 445
Phe Asp His Ser Asn Asp Asn Ile Ser Val Glu Arg Tyr Asn Ile Tyr
450 455 460
Gln Asp Lys Gly Glu Asn Pro Ile Leu Ser Ile Gln Thr Glu Lys Asp
465 470 475 480
Gly Ser Gly Leu Ser Pro Ser Lys Pro Glu Gly Thr Val Glu Val Ser
485 490 495
Ile Glu Asn Leu Glu Gln Val Thr Asp Tyr Glu Phe Arg Val Ser Ala
500 505 510
Val Asp Glu Ala Gln Asn Glu Ser Asp Lys Ser Asn Val Leu Lys Val
515 520 525
Lys Thr Lys Asp Thr Glu Pro Pro Glu Leu Lys Gly Ser Leu Arg Gly
530 535 540
Lys Ala Leu Ala Ser Thr Ala Ala Arg Leu Asn Trp Pro His Pro Thr
545 550 555 560
Asp Asn Val Gly Val Lys Glu Val Lys Ile Arg Arg Gln Lys Ser Ala
565 570 575
Ser Thr Phe Gly Ile Asp Ser Met Gly Ile Val Ala Thr Ser Glu Glu
580 585 590
Ile Phe Pro Val Pro Gly Asp Thr Asn His Phe Thr Asp Phe Met Ile
595 600 605
Glu Lys Gly Lys Thr Tyr Thr Tyr Ser Met Thr Ala Leu Asp Glu Ala
610 615 620
Gly Asn Glu Ser Glu Arg Ser Asn Asn Val Val Ile Lys Thr Asn Asp
625 630 635 640
Asn Asp Asp Asp Lys Lys Asn Glu Asp Arg Ala Glu Asp Ile Ser Ser
645 650 655
Ser Asn Ala Thr Leu Asn Trp Asn Gly Ala Phe Lys Gly Ile Ser Ala
660 665 670
Ser Gly Phe Gln Leu Phe Gly Trp Leu Lys Gly Ala Thr Gly Trp Leu
675 680 685
Phe Ser Gly Val Thr Ser Val Val Gly Ser Ala Ser Ser Phe Val Val
690 695 700
Asp Leu Thr Pro Ala Leu Leu His Lys Phe Val Val Ile Pro Val Asp
705 710 715 720
Glu Glu Gly Asn Pro Leu His Asp Gly Leu Glu Ile Ser Val Glu Ala
725 730 735
Leu Arg Glu Lys Leu Ser Ala Val Ala Val Asn Gln Leu Ile Asp Leu
740 745 750
Gly Thr Asp Val Gln Thr Val Lys Tyr Asp Asn Phe Val Lys Asp Val
755 760 765
Lys Leu Gly Gly Lys Glu Ile Pro Val Thr Asp Tyr Thr Val Met Ala
770 775 780
Leu Asn Asp Ile Thr Thr Asp Ile Val Gly Glu Gln Lys Ala Thr Ile
785 790 795 800
Arg Val Thr Leu Lys Ala Asp Pro Leu Asn Phe Val Asp Ile Asp Val
805 810 815
Leu Val His Val Glu Trp Gly Asn Ser Ala Ile Ala Tyr Gly Ala Phe
820 825 830
Gly Asp Gly Arg Thr Ser Val Ala Phe Pro Phe Ile Gln Gly Asp Ser
835 840 845
Pro Val Ile Ala Ala Val Ser Gly Leu Gly Asn Asp Asn Asn Val Ile
850 855 860
His Ser Tyr Phe Lys Glu Glu Leu Tyr Tyr Lys Phe Asp Trp Phe Asp
865 870 875 880
Leu Ser Lys Leu Ser Ser Phe Leu Met Asp Glu Asn Lys Asn Gly Asn
885 890 895
Val His Ile Glu Ala Met Gly Asp Asp Arg Lys Gln Asp Ala Leu Lys
900 905 910
Lys Trp Gly Thr Asn Gln Lys Gln Thr Val Asn Asp Gly Asp Ile Val
915 920 925
Arg Ala Trp Gln Ala Glu Pro Thr Lys Asn Trp Leu Tyr Glu Asn Gly
930 935 940
Gln Gln Asn Lys Tyr Asn Asn Gly Gln Gln Ala Val Tyr Tyr Glu Ile
945 950 955 960
Thr Lys Gln Gly Tyr Gln Pro Leu Arg Phe Asn Gln Leu Lys Pro Lys
965 970 975
Glu Gln Ile Ile Tyr Thr Asp Thr Pro Asn Gln Lys Leu Asp Lys Asn
980 985 990
Ile Lys Lys Tyr Leu Ser Val Glu Asp Tyr Lys Thr Ile Glu Val Lys
995 1000 1005
Lys Phe Val Thr Tyr Pro Asp Thr Ser Lys Pro Gly Lys Thr Met
1010 1015 1020
Gly Val Ile Gln Val Glu Glu Ile Leu Arg Ser Gly Lys Lys Ile
1025 1030 1035
Ala Tyr Asp Tyr Thr Val Pro Phe Val Ile Glu Pro Lys Leu Glu
1040 1045 1050
Ile Met Gly Gly Thr Phe Gln Leu Val Thr Ala Leu Asn Asn Val
1055 1060 1065
Ser Val Val Ser Lys Ser Gly Asp Ser Val Val Leu Thr Asn Asn
1070 1075 1080
Lys Asn Thr Ala Asn Gln Asp Trp Gly Phe Val Tyr Asn Val Lys
1085 1090 1095
Lys Asp Ala Tyr Gln Ile Lys Ser Ala Ser Asn Glu Glu Leu Val
1100 1105 1110
Leu Thr Trp Thr Glu Glu Asp Phe Val Arg Val Asn Ser Asn Glu
1115 1120 1125
Asp Lys Asp Glu Gln Tyr Trp Val Leu Glu Asp Ala Asn Asp Gly
1130 1135 1140
Ser Phe Phe Ile Gln Asn Lys Lys Asn Pro Asn Leu Val Leu Asp
1145 1150 1155
Val Asp Glu Ala Asn Pro Gln Glu Gly Thr Arg Ile Lys Val Val
1160 1165 1170
Glu Arg His Lys Asp Pro Ser Ser Pro His Ile Lys Ala Gln Lys
1175 1180 1185
Phe Lys Leu Gln Val Val Asn Leu Ser Glu Glu Asn Val Ser Phe
1190 1195 1200
Gln Ala Ile Asn Phe Trp Lys Ala Arg Phe Ser Asp Asp Asn Ala
1205 1210 1215
Tyr Arg Gly Phe Gln Gln Tyr Pro Asp Glu Gln Asn Gln Val Trp
1220 1225 1230
Asn Gly Leu Tyr Trp Lys Gln Gly Glu Ala Asp Asn Glu Gly Gln
1235 1240 1245
Asp Lys Phe Leu Phe Leu Phe Tyr Lys Leu Asp Asp Asp Arg Val
1250 1255 1260
Val Ile Val Asn Arg Lys Thr Gly Glu Val Leu Glu His Val Tyr
1265 1270 1275
Arg Asp Glu Lys Asp Thr Ser Val Val Leu Gln Pro Tyr Asn Gly
1280 1285 1290
Lys Glu Ser Gln Val Phe Lys Lys Arg Met Trp Ala Ser Asp Arg
1295 1300 1305
Asp Thr Phe Gln Leu Leu Val Asp Asn Asp Lys Arg Ile Val Asp
1310 1315 1320
Val Gln Asn Asp Gln Val His Ser Trp Ala Lys Ile Thr Ser Trp
1325 1330 1335
Asp Thr Glu Lys His Glu His Asn Glu Tyr Thr Arg Leu Phe His
1340 1345 1350
Asn Pro Asn Gly Glu Lys Lys Val Ile Leu Pro Ser Leu Ser Pro
1355 1360 1365
Thr Asn Glu Leu Gly Pro Ile Pro Glu Leu Pro Gly Phe Asn Asp
1370 1375 1380
Pro Leu Pro Glu Asn Glu Asp Ala Lys Arg Ala Val Ile Gly Ser
1385 1390 1395
Ala Leu Ile Pro Cys Ile Val Val Asn Asp Asn Ile Ser Leu Glu
1400 1405 1410
Asp Lys Ile Lys Gln His Pro Tyr Tyr Ile Leu Glu Tyr Arg Gln
1415 1420 1425
Tyr Trp His Leu Leu Trp Ser Asp Val Phe Thr Ala Gly Ser Asn
1430 1435 1440
Lys Ile Lys Thr Glu Val Thr Gly Ile Pro Ser Glu Ala Gln Glu
1445 1450 1455
Lys Met Lys Glu Val Val Gly Ala Ser Ile Arg Thr Asp Ile Gly
1460 1465 1470
Leu Arg Phe Thr Asp Lys Ser Glu Pro Phe Gly Lys Gln Ile Thr
1475 1480 1485
Ser Gly Leu Asn Val Lys His Ser Tyr Asp Tyr Glu Leu Gly Glu
1490 1495 1500
Thr Thr Thr Lys Lys His Val Glu Asn Pro Asn Asp Phe Thr Val
1505 1510 1515
Arg Tyr Ala Gln Tyr Ala Arg Ala His Glu Phe Val Leu Lys Arg
1520 1525 1530
Pro Asp Gly Gln Val Val Glu Glu Pro Trp Val Ala Val Asp Glu
1535 1540 1545
Asn Asp Met Tyr Leu Lys Met Tyr Lys Lys Pro
1550 1555
<210> 167
<211> 409
<212> БЕЛОК
<213> Pseudomonas sp.
<400> 167
Met Glu Ile Asp Leu Glu Ala Ala Ala Lys Glu Leu Glu Ala Arg Gly
1 5 10 15
Glu Gln Val Phe Ser Lys Leu Glu Gly Leu Ser Ser Ile Val Asp Ala
20 25 30
Ala Arg Phe Val Pro Val Gln Tyr Phe Glu Lys Lys Val Glu Asn Ile
35 40 45
Asp Asp Glu Thr Phe Ile Val Lys Lys Asn His Val Val Met Ile Lys
50 55 60
Arg Tyr Val Arg Ala Gly Leu Ser Leu Pro Val Asn Ile Ser Glu Ile
65 70 75 80
Glu Thr Tyr Leu Gly Tyr Lys Asn Val Gly His Arg Leu Leu Glu Pro
85 90 95
Thr Ser Met Gln Gln Phe Tyr Gln Glu Ile His Ser His Ala Leu Ser
100 105 110
Trp Ser Thr Leu Glu Arg Glu Thr Lys Asn Val Gly Asn Arg Leu Asp
115 120 125
Leu Phe Ala Ser Asn Phe Phe Glu Thr Gly Ser Ala Ile Ile Asn Leu
130 135 140
Leu Lys Glu Thr Glu Thr Tyr Lys Arg Val Glu Gly Asn Ile Asp Ser
145 150 155 160
Leu Ser Asp Asp Glu Lys Thr Gln Leu Ala Ser Ile Pro Leu Ser Ser
165 170 175
Thr Asp Thr Lys Arg Val Glu Ser Leu Arg Lys Ala Leu Lys Phe Ala
180 185 190
Lys Lys Asp Val Ala Glu Phe Tyr Leu Ala Val Ala Asp Val Asn Ala
195 200 205
Leu Ala Val Glu Phe Ser Arg Lys Ile Ser Asp Glu Leu Leu Ile Lys
210 215 220
Leu Ala Leu Lys Met Asn Ala Val Asp Glu Glu Ser Ile Asp Lys His
225 230 235 240
Glu Gly Ile Asp Glu Leu Arg Lys Gln Ile Lys Glu Leu Asp Glu Thr
245 250 255
Ile Ala Glu Lys Gln Thr Glu Tyr Asp Tyr Gln Val Gly Tyr Ala Phe
260 265 270
Thr Gly Leu Val Phe Gly Pro Leu Gly Leu Ile Val Thr Gly Gly Ile
275 280 285
Phe Gly Ala Asp Ala Glu Lys Thr Arg Ala Gln Lys Asn Glu Leu Leu
290 295 300
Glu Gln Lys Asn Arg Leu Val Val Gln Met Ser Ala Ala Arg Leu Ser
305 310 315 320
Gln Asn Leu Ile Thr Leu Thr Thr Asn Phe Ser Glu Met Arg Ala Leu
325 330 335
Met Lys Asp Ala Glu Asn Gly Ile Lys Cys Ile Glu Asp Val Leu Gly
340 345 350
Ile Val Trp Asn Ser Leu Gly Val Ser Leu Asp Gly Phe Glu Asp Gly
355 360 365
Ala Ser Asp Leu Asp Leu Ile Leu Leu Val Leu Asn Leu Gln Ala Ile
370 375 380
Phe Asn Pro Trp Lys Thr Ile Gln Gly His Ala His Ala Leu Ser Thr
385 390 395 400
Val Phe Asn Gln Val Val Glu Gly Glu
405
<210> 168
<211> 589
<212> БЕЛОК
<213> Bacillus sp.
<400> 168
Met Lys Tyr Lys Asn Arg Lys Asp Ala Lys Arg Lys Tyr Lys Lys Val
1 5 10 15
Ala Leu Ala Thr Val Ala Thr Met Thr Leu Gly Val Ser Val Val Gly
20 25 30
Asn Thr Ala Ser Val Phe Ala Asp Glu Thr Ser Thr Arg Thr Arg Gln
35 40 45
Thr Ala Leu Glu Ser Leu Gly Thr Ser Gly Gly Ala Gly Ala Thr Ser
50 55 60
Ser Lys Leu Glu Glu Leu Leu Thr Met Asp Asn Leu Lys Lys Val Gly
65 70 75 80
Thr Leu Ser Gly Asp Val Leu Lys Val Ala Tyr Lys Asp Ala His Glu
85 90 95
Asn Asn Gly Asn Tyr Asn Asn Thr Phe Arg Thr Leu Ala Met Ser Gly
100 105 110
Thr Ala Leu Ile Pro Tyr Gly Gly Val Ile Ile Ser Pro Leu Ile Gly
115 120 125
Leu Leu Trp Pro Glu Asn Val Glu Ala Gln Lys Asn Gln Met Lys Lys
130 135 140
Leu Ile Asp Asp Leu Asn Asn Met Met Asp Gln Lys Ile Thr Asp Asn
145 150 155 160
Glu Ile Ala Thr Ile Glu Ser Glu Val Asn Ile Leu Lys Glu Met Leu
165 170 175
Gln Lys Phe Glu Lys Asn Ile Asn Met Asn Ile Ser Ala Gly Ser Phe
180 185 190
Tyr Ser Leu Thr Thr Lys Glu Gln Ala Leu Ala Gln Asp Ala Lys Asp
195 200 205
Ile Asn Asn Lys Phe Ser Asp Ile Ile Gly His Cys Lys Gln Lys Leu
210 215 220
Gln Val Ile Pro Ala Leu Pro Ile Phe Thr Ala Met Ala Thr Ala His
225 230 235 240
Leu Glu Phe Leu Tyr Phe Leu Glu Lys Asn Gly Thr Gly Pro Lys Leu
245 250 255
Gln Tyr Thr Thr Asp Ala Leu Asn Thr Leu Leu Lys Gly Glu Asp Asn
260 265 270
Glu Asp Phe Gly Lys Arg Ile Lys Asn Leu Ala Glu Thr Tyr Val Asn
275 280 285
His Val Gln Lys Thr Tyr Glu Leu Gly Lys Gln Gln Phe Asn Lys Lys
290 295 300
Met Asp Asp Thr Ala Lys Asn Thr Pro Gly Gly Asn Val Asn Ser Thr
305 310 315 320
Ser Glu Gln Glu Thr Met Ser Lys Met Gln Lys Tyr Ile Phe Asp Leu
325 330 335
His Pro Val Ala Asn Asn Ala Asp Glu Lys Arg Lys Gln Met Gln Val
340 345 350
Ala Leu Asn Asn Tyr Val Lys Leu Arg Asn Asp Arg Glu Gln Tyr Tyr
355 360 365
Lys Asn Thr Ala Asn Asn Glu Val Phe Gln Leu Leu Ala Ile Gly Lys
370 375 380
Trp Lys Gln Glu Gly Asp Lys Trp Tyr Phe Ile Asp Thr Lys Gly Glu
385 390 395 400
Lys Lys Thr Gly Trp Ile Gln Leu Gly Glu Lys His Tyr Tyr Leu Ser
405 410 415
Pro Lys Asn Gly Ile Met Val Lys Gly Phe Val Thr Ile Glu Gly Lys
420 425 430
Pro Tyr Gln Phe Asn Asp Ser Gly Glu Leu Ile Phe Ser Glu Gly Thr
435 440 445
Tyr Lys Ile Val Tyr Ser Lys Ala Asn Lys Val Val Asp Phe Gly Ala
450 455 460
Tyr Gly Glu Gln Pro Val Ile Trp Asp Tyr His Pro Asp Trp Asn Thr
465 470 475 480
Pro Asn Thr Asn Gln Gln Trp Glu Phe Lys Tyr Asp Ala Ser Lys Asn
485 490 495
Ala Tyr Gln Ile Ile Asn Lys Gln Asn Asn Lys Val Leu Ala Tyr Asn
500 505 510
Thr Thr Ser Gly Thr Asp Tyr Thr Ala Leu Val Thr Glu Asn Gln His
515 520 525
Lys Ala Glu His Tyr Trp Thr Leu Glu Asp Ala Val Asp Gly Asn Ile
530 535 540
Leu Leu Val Asn Phe Ala Asn Lys Asn Lys Val Leu Asp Val Tyr Gly
545 550 555 560
Glu Glu Thr Ala Asn Gly Ser Lys Val Tyr Val Arg Asp Lys Leu Asn
565 570 575
Asn Ser Met Ala Gln Lys Phe Lys Leu Ile Lys Leu Asn
580 585
<210> 169
<211> 514
<212> БЕЛОК
<213> Bacillus sp.
<400> 169
Met Glu His Thr Gln Thr Ser Gln Ser Ile Ile Ile Lys Lys Ala Leu
1 5 10 15
Glu Lys Ile Lys Asp Pro Glu Thr Gln Ser Ala Val Lys Ser Leu Ile
20 25 30
Gly Asp Gly Ile Lys Gln Ala Tyr Glu Asp Thr Lys Ser Gly Asp Phe
35 40 45
Thr Arg Thr Ile Arg Thr Leu Thr Leu Gly Ala Leu Thr Leu Ile Pro
50 55 60
Tyr Gly Gly Ala Phe Val Ser Gln Ile Leu Gly Val Leu Trp Glu Thr
65 70 75 80
Lys Glu Thr Asn Pro Leu Lys Val Leu Glu Asp Gln Ile Lys Asp Leu
85 90 95
Glu Gly Lys Val Lys Ala Leu Asn Ser Ser His Leu Lys Ala Lys Tyr
100 105 110
Asp Val Leu Met Lys Glu Leu Gln Arg Phe Glu Asp Gly Met Asn His
115 120 125
Gln Pro Gly Thr Ala Ser Phe Tyr Asp Ala Gly Ser Ile Glu Gln Thr
130 135 140
Asn Arg Met Ser Ala Gln Leu Ile Asn Ser Lys Phe Lys Asp Leu Ile
145 150 155 160
Lys Ala Cys Glu Ile Lys Ile Leu Arg Thr Glu Glu Leu Pro Ile Tyr
165 170 175
Thr Leu Val Ala Thr Ala His Leu Val Phe Leu Gln Ile Ile Gln Tyr
180 185 190
Asn Trp Lys Ser Thr Lys Leu Arg Ile Asp Gln His Ser Tyr Asp Leu
195 200 205
Tyr Tyr Lys Asp Asp Ile Gln Lys Leu Pro Gly Glu Tyr Leu Asn His
210 215 220
Val Met Asn Thr Tyr Lys Thr Lys Lys Asp Glu Tyr Gln Lys Glu Met
225 230 235 240
Gln Asp Val Val Lys Ser Ala Pro Ile Ser Tyr Thr Ser Asp Asp Gly
245 250 255
Lys Leu Asn Leu Lys Val Met Gln Asp Lys Tyr Asp Glu Leu Tyr Lys
260 265 270
Lys Phe Glu Ala Asp Gly Arg Ile Ala Ile Trp Ser Gly Gln Val Asp
275 280 285
Thr Met Asn Lys Leu Arg Lys Ala Ile Asn Asp Tyr Ile Thr Leu Met
290 295 300
Thr Lys Lys Asn Lys Tyr Tyr Tyr Glu Thr Ala Gly Asn Glu Ala Phe
305 310 315 320
Gln Leu Leu Ala Leu Gly Lys Trp Val Gln Glu Asn Gly Asn Arg His
325 330 335
Phe Val Asp Asn Ala Gly Gln Lys Lys Thr Asn Trp Leu Gln Leu Gly
340 345 350
Asp Lys Trp Tyr Tyr Phe Ser Pro Thr Thr Gly Gln Met Val Thr Gly
355 360 365
Lys Phe Asp Ile Glu Asn Lys Thr Tyr Tyr Phe Lys Pro Asn Thr Gly
370 375 380
Glu Met Glu Thr Ser Trp Ile Gln Asp Gly Asp Thr Lys Tyr Tyr Phe
385 390 395 400
Ser Pro Ala Asp Gly Thr Lys Asn Tyr Ala Gly Thr Ser Phe Lys Lys
405 410 415
Gly Glu Met Met Thr Gly Phe Val Gln Leu Ile Asn Lys Asp Gly Glu
420 425 430
Asp Tyr Tyr Tyr Phe Ser Pro Thr Asn Asp Ala Thr Asn Asp Tyr Gln
435 440 445
Lys Lys Lys Thr Phe Lys Lys Gly Glu Met Trp Thr Gly Trp Leu Glu
450 455 460
Asp Ser Gly Lys Trp Tyr Tyr Met Asn Lys Gly Asp Lys Ala Thr Arg
465 470 475 480
Asp Lys Gly Ile Met Leu His Asp Glu Lys Ile Glu Leu Asp Asn Gly
485 490 495
Lys Gly Val Lys Lys Trp Tyr Asn Phe Asp Tyr Lys Gly Val Gly Thr
500 505 510
Asn Ser
<210> 170
<211> 403
<212> БЕЛОК
<213> Bacillus sp.
<400> 170
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Thr Ser Asp Ala Ala Ser Leu Glu
20 25 30
Lys Asn Arg Tyr Tyr Ser Val His Ser Lys Lys Asp Phe Asn Leu Thr
35 40 45
Trp Asp Val Tyr Gly Ser Lys Asp Tyr Asp Asn Ala Gly Ile Leu Leu
50 55 60
Tyr Lys Ser Asn Gly Gln Asp Asn Gln Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ser Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Gly Asp Pro Ile Ala Ile Asp Asp Gly Val Arg Arg Met Thr
100 105 110
Leu Phe Asp Asn Glu Gly Leu Leu Gln Lys Gly Trp Thr Gly Ala Ser
115 120 125
Ala Glu Gln Trp Tyr Leu Arg Asp Gln Gly Asn Asn Asn Tyr Glu Phe
130 135 140
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Trp Thr Gly Thr
145 150 155 160
Leu Ala Gln Asn Trp Thr Tyr Val Asp Leu Asp Asp Ala Asn Pro Ser
165 170 175
Asp Asn Asp Arg Val Phe Thr Ile Ser Lys Ser Gly Leu Pro Gly Gly
180 185 190
Ile Gln Ile Pro Gly Phe Asn Phe Asp His Gly Thr Phe Ser Leu Gln
195 200 205
Gln Leu Pro Ala Thr Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Gly
210 215 220
Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly
225 230 235 240
Ala Ser Leu Val Pro Cys Ile Met Val Lys Asp Asn Gln Ala Ser Asp
245 250 255
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu
260 265 270
Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg
275 280 285
Asn Tyr Ser Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln Lys Met
290 295 300
Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Leu Gly Leu Lys Phe
305 310 315 320
Gly Gln Gln Ser Ala Ser Ile Lys Thr Glu Val Ser Arg Thr Leu Gln
325 330 335
Thr Glu Ile Ser Thr Thr Asn Thr Glu Ala Thr Glu Glu Thr Val Thr
340 345 350
Ser Thr Val Ser Ser Glu Ala Gly Lys Thr Thr Gly Phe Thr Glu Tyr
355 360 365
Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val
370 375 380
Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile Ala Arg Lys
385 390 395 400
Asn Ala Gln
<210> 171
<211> 298
<212> БЕЛОК
<213> Bacillus sp.
<400> 171
Met Ala Ile Phe Asp Leu Asp Thr Tyr Leu Leu Asp Leu Phe Lys Lys
1 5 10 15
Val Val Asp Ser Asp Leu Glu Pro Ala Leu Arg Val Tyr Thr Ser Asp
20 25 30
Leu Ala Val Lys Asn Glu Glu Ile Tyr Gly Phe Glu Ile Lys Glu Ser
35 40 45
Lys Pro Gln Gly Thr Met Phe Met Gly Glu Ser Ile Leu Asn Asn Arg
50 55 60
Thr Asn Glu Thr Gln Thr Ile Leu Ser Asp Ser Phe Thr Lys Thr Ile
65 70 75 80
Thr Asp Ser Val Thr Leu Ser Val Thr Asn Gly Ile Thr Thr Gly Val
85 90 95
Asn Ile Ser Ile Ser Gly Lys Ile Phe Gly Met Gly Ala Glu Thr Ser
100 105 110
Met Ser Ile Glu Val Ser Thr Ser Thr Thr Asn Glu Gln Thr Asn Glu
115 120 125
Glu Ser Ile Ala Tyr Thr Val Pro Ser Gln Thr Val Val Val Pro Ala
130 135 140
Gln Thr Thr Tyr Tyr Val His Thr Ala Leu Glu Arg Asn Arg Leu Glu
145 150 155 160
Gly Ile Val Arg Leu Arg Ala Asp Leu Ser Gly Glu Ile Thr Leu Tyr
165 170 175
Val Gln Phe Thr Glu Asp Leu Lys Leu Pro Leu Ile Thr Ile Asp Ile
180 185 190
Tyr Asp Phe Val Asn His Phe Gln Ser Ser Leu Pro Leu Pro Ser Gly
195 200 205
Ile Ser Leu Asn His Asn Thr Lys Ala Ile His Phe Glu Gly Ile Ala
210 215 220
Glu Tyr Leu Tyr Gly Thr Gly Thr Lys Phe His Val Thr Val Thr Asp
225 230 235 240
Lys Pro Ser Ser Gln Gly Thr His Glu His Lys Ser Phe Asp Ala Lys
245 250 255
Thr Gly Leu Gly Thr Tyr Pro Ile Gly Leu Asn Gly Lys Lys Leu Arg
260 265 270
Leu Asp Leu Asn Asp Leu Lys Glu His Met Asn Pro Lys Asp Phe Glu
275 280 285
Lys Leu Lys Glu Leu Gln Asn Lys Ile Val
290 295
<210> 172
<211> 261
<212> БЕЛОК
<213> Неизвестный
<220>
<223> неизвестный организм из образца окружающей среды
<400> 172
Val Val His Ala Gly Gln Ser Arg Val Arg Gly His Val Arg Ser Arg
1 5 10 15
Leu Val His Arg Glu Arg Leu Gln Val Thr Ser Arg Thr Glu Cys Glu
20 25 30
Thr Glu Leu Pro Val Arg Gly Arg Leu Cys Ile Leu Ala Arg Arg Gln
35 40 45
Leu Ile Thr Ser Thr Pro Ser Leu Pro Ile Pro Ala Gly Ala Ala Met
50 55 60
Val Asp Arg Ala Thr Ser Thr Arg Ala Ala Phe Lys Ala Val Val Glu
65 70 75 80
Val Gly Pro Asp Ala Leu Asp Arg Ala Arg Ala Ala Glu Ala Val Leu
85 90 95
Gln Glu Ala Ile Ala Pro Ala Ala Gly Asp Phe Asp Phe Gly Ala Val
100 105 110
Arg Ser Ala Ala Arg Ala Leu Pro Asn Ser Ser Val Val Lys Ile Ile
115 120 125
Pro Gly Phe Gly Leu Gln Glu Thr Ala Pro Val Gln Val Met Val Leu
130 135 140
Thr Leu Lys Gln Ala Val Arg Gln Ala Leu Val Glu Pro Phe Glu Asn
145 150 155 160
Pro Ala Phe Trp Asp Arg Ala Asp Ala Ala Leu Ala Gln Val Phe Leu
165 170 175
Gly Leu Gly Glu Gln Glu Gly Ala Ala His Leu Ser Tyr Gln Asp Ala
180 185 190
Ala Asp Gly Thr Arg Tyr Phe Tyr Asn Leu Leu Phe Ala Leu Gln Asp
195 200 205
Glu Glu Thr Gly Asn His Leu Tyr Val Val Ala Phe Arg Val Glu Val
210 215 220
Ser Val Gly Ala Asp Lys Ala Glu Leu Leu Ser Leu Gly Ala Glu Asp
225 230 235 240
Thr Ala Ala Val Thr Ile Arg Leu Asp Ala Ile Ser Val Arg Gln Glu
245 250 255
Leu Ala Ala Thr Val
260
<210> 173
<211> 391
<212> БЕЛОК
<213> Bacillus sp.
<400> 173
Leu Pro Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys Lys
1 5 10 15
Val Met Ala Gly Leu Leu Val Gly Ala Thr Ser Leu Ser Ile Trp Ala
20 25 30
Pro Ala Ser Gln Ala Ala Pro Ala Asn Asn Ala Tyr His Ser Ile His
35 40 45
Leu Ala Ala Asn Ala Asn Leu Lys Trp Asp Val Tyr Gly Asp Gly Thr
50 55 60
Tyr Asp Asp Ala Gly Ile Leu Leu Tyr Asp Gly Ser Leu Gly Asp Asn
65 70 75 80
Glu Gln Phe Leu Phe Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile Val
85 90 95
Asn Lys Asn Ser Gly Lys Ala Val Thr Thr Gly Ser Gly Trp Ile Ala
100 105 110
Gly Asn Thr Met Ser His Asn Pro Glu Ala Leu Lys Gln Ala Lys Trp
115 120 125
Thr Gly Ala Asn Thr Asn Lys Trp Tyr Leu Arg Asp Lys Gly Asn Asn
130 135 140
Asn Tyr Glu Ile Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Gly
145 150 155 160
Arg Ala Gly Ile Ala Gly Pro Arg Phe Val Asp Leu Asp Asp Ser Asn
165 170 175
Pro Ser Asp Phe Asp Arg Leu Phe His Ile Pro Ala Ala Ala Arg Ser
180 185 190
Thr Phe Ser Leu Pro Thr Leu Pro Thr Ile Gly Thr Arg Pro Asp Ala
195 200 205
Pro Asn Tyr Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn
210 215 220
Ser Val Val Val Gly Ala Ser Leu Leu Pro Cys Ile Met Val Asn Asp
225 230 235 240
Ser Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr
245 250 255
Leu Val Lys Glu Glu Tyr Trp Asp Arg Thr Phe Ser Lys Val Ile Gln
260 265 270
Pro Gly Leu Ser Glu Ser Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val
275 280 285
Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ser Asp
290 295 300
Leu Gly Ile Lys Phe Gly Asp Val Ser Ala Ser Leu Lys Ser Glu Ile
305 310 315 320
Ser Arg Thr Ile Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr
325 330 335
Glu Glu Thr Arg Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr
340 345 350
Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala
355 360 365
Asp Gly Ser Thr Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile
370 375 380
Thr Val Thr Arg Lys Thr Ser
385 390
<210> 174
<211> 372
<212> БЕЛОК
<213> Bacillus sp.
<400> 174
Val Met Ser Asn Glu Asn Asn Met Asn Thr Ser Met Ile Asn Asp Glu
1 5 10 15
Asn Leu Ile Pro Ser Glu Val Leu Ala Ser Gly Lys Gln Ile Ser Phe
20 25 30
Pro Asp Phe Asn Phe Tyr Lys Ile Arg Thr Phe Cys Gly Lys Tyr Val
35 40 45
Asp Ile Arg Gly Gly Ser Thr Asp Asp Asp Ile Pro Ala Val Gln Tyr
50 55 60
Thr Gly Arg Asn Val Asp Asn Gln Lys Phe Leu Ile Phe Thr Leu Asp
65 70 75 80
Asn Asn Tyr Ser Val Ile Ala Ala Lys His Ser Gly Lys Val Leu Asp
85 90 95
Ile Ile Ser Leu Leu Phe Pro Phe Ala Leu Ile Gln Tyr Tyr Phe Lys
100 105 110
Asn Gly Asp Asn Gln Lys Phe Phe Ile Ala Asn Asp Gly Thr Ile Ala
115 120 125
Val Lys Ser Asp Gly Lys Val Leu Asp Ile Pro Asn Gly Ser Thr Lys
130 135 140
Asp Asn Val Ile Ile Ile Pro Tyr Gln Tyr His Gly Ser Ser Asn Gln
145 150 155 160
Lys Phe Thr Leu Glu Lys Tyr Gly Ser Ile Ser Ile Lys Pro Pro Thr
165 170 175
Thr Gly Thr Leu Pro Pro Ala Pro Asp Phe Lys Thr Asn Asp Ile Asn
180 185 190
Glu Glu Leu Pro Asp Asp Thr Pro Pro Val Ile Thr His Ala Thr Tyr
195 200 205
Met Pro Tyr Phe Met Val Lys Asp Pro Tyr Tyr Asn Ala Gln Gln Lys
210 215 220
Ile Gln Asn Thr Pro Tyr Tyr Ile Leu Val Arg Arg Gln Tyr Trp Gln
225 230 235 240
Lys Val Thr Gln Arg Ile Leu Ala Pro Ser Asp Thr Tyr Asp Tyr Ser
245 250 255
Glu Thr Thr Gly Val Ser Arg Thr Asp Gln Thr Ser Met Thr Glu Thr
260 265 270
Thr Glu Ile Ser Ile Gly Ala Asp Leu Gly Phe Met Phe Lys Gly Phe
275 280 285
Ser Val Gly Leu Ser Ala Ser Ile Thr Lys Ser Leu Ser Val Thr Lys
290 295 300
Ser Thr Ser Thr Thr Glu Ser Thr Glu Ile Thr Gln Lys Val Val Tyr
305 310 315 320
Thr Asn Pro Phe Lys His Gln Ile Ala Tyr Ala Lys Tyr Met Leu Ile
325 330 335
Asn Glu Tyr Tyr Val Thr Arg Ala Asn Gly Glu Met Ile Thr Ser Gly
340 345 350
Asp Ala Ser Tyr Trp Lys Val Pro Asp Pro Ser Arg Thr Val Ala Arg
355 360 365
Ile Ile Pro Arg
370
<210> 175
<211> 348
<212> БЕЛОК
<213> Bacillus sp.
<400> 175
Leu Met Pro Thr Ile Gln Ala Gly Ala Ala Tyr Asn Ile Ala Leu Lys
1 5 10 15
Ala Asn Gln Asn Leu Val Phe Asp Val Tyr His Asn Ile Met Asp Ser
20 25 30
Gly Gly Ile Ile Cys Leu Tyr Asn Lys Gly Ser Asn Tyr Leu Asn Gln
35 40 45
Gln Tyr Phe Phe Phe Ala Leu Asp Gly Gly Arg Tyr Ala Ile Val Pro
50 55 60
Arg Ser Ser Gly Lys Pro Val Glu Pro Ile Phe Ser Asp Ile Arg Arg
65 70 75 80
Ile Lys Pro Leu Gln Gln Arg Ser Trp Gly Gly Thr Asn Arg Gln Gln
85 90 95
Trp Tyr Leu Leu Asp Ala Pro Gly Ser Ser Val Glu Ile Phe Asn Gln
100 105 110
Asp Tyr Gly Gln Val Ala Ser Tyr Gly Lys Asp Gln Glu Leu Glu Tyr
115 120 125
Pro Asp Leu Asp Thr Ser Asn Pro Ser Asp Pro Asn Arg Thr Trp Tyr
130 135 140
Leu Gln Pro Val Tyr Gln Ala Thr Leu Pro Gln Leu Val Ala Thr Lys
145 150 155 160
Pro Ile Gly Ser Pro Pro Gln Tyr Ala Gly Asp Pro Tyr Gln Val Leu
165 170 175
Pro Asp Gln Ser Glu Val Thr Asn Val Ser Asn Ala Leu Ile Pro Cys
180 185 190
Ile Leu Val Asn Asp Gly Ser Leu Ser Asp Ala Thr Lys Val Gln Gln
195 200 205
Ser Pro Tyr Tyr Leu Met Ile Lys Gln Gln Phe Trp Gln Lys Ala Ala
210 215 220
Phe Gly Thr Val Ala Pro Asn Ser Lys Arg Ser Phe Ser Phe Lys Thr
225 230 235 240
Gly Ser Thr Ser Ile Asp Gln Lys Ser Met Ser Asp Thr Leu Ser Ile
245 250 255
Thr Val Gly Gly Asp Leu Gly Leu Gln Phe Lys Gly Ala Ser Ala Ser
260 265 270
Ala Lys Phe Glu Ile Ser Arg Gln Ile Gln Thr Val Val Ser Ser Thr
275 280 285
Asn Glu Gln Met Ser Glu Tyr Thr Asp Thr Ile Glu Phe Asp Asn Ser
290 295 300
Ser Gly Thr Thr Leu Lys Gly Ile Ser Ile Tyr Gln Leu Val Thr Arg
305 310 315 320
Tyr Ala Leu Tyr Arg Thr Asn Gly Ser Ala Val Ser Asp Pro Trp Lys
325 330 335
Phe Ile Asp Asn Lys Ser Ala Val Met Val Val Ser
340 345
<210> 176
<211> 397
<212> БЕЛОК
<213> Bacillus sp.
<400> 176
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ala Ser Gln Ala Ala Ala Pro Asp
20 25 30
Asn Asn Arg Tyr Tyr Ser Val His Leu Ala Ala Asp Thr Ala Leu Lys
35 40 45
Trp Asp Val Tyr Gly Asn Gly Thr Tyr Asp Asp Ala Gly Ile Leu Leu
50 55 60
Tyr Asn Gly Ser Leu Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Ala Val
85 90 95
Thr Ile Gly Gly Gly Trp Val Gly Asp Asn Ala Phe Ser Tyr Asn Arg
100 105 110
Glu Gly Leu Thr Gln Ser Lys Trp Thr Gly Ala Ala Ala Asn Lys Trp
115 120 125
Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly
130 135 140
Tyr Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile Thr Gly Pro Lys
145 150 155 160
Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn Asp Arg Leu Phe
165 170 175
Lys Ile Gln Lys Ser Ala Leu Pro Leu Pro Asp Gly Val Gln Leu Pro
180 185 190
Gly Trp Pro Glu Tyr Gly Thr Phe Ser Leu Pro Gln Leu Pro Ala Thr
195 200 205
Gly Asn Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Ile Asp Gln Gln
210 215 220
Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Leu Pro
225 230 235 240
Cys Ile Met Val Asn Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile His
245 250 255
Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr
260 265 270
Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu Ser Tyr Ser Tyr Lys
275 280 285
Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser
290 295 300
Met Lys Phe Gly Ala Asp Leu Gly Ile Lys Phe Gly Asp Val Ser Ala
305 310 315 320
Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile Ser Thr
325 330 335
Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr Thr Ala Thr Gly
340 345 350
Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys
355 360 365
Tyr Thr Leu Lys Arg Ala Asp Gly Ser Thr Val Ser Asp Pro Trp Val
370 375 380
Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys Thr Ser
385 390 395
<210> 177
<211> 344
<212> БЕЛОК
<213> Ensifer sp.
<400> 177
Met Asn Glu Val Leu Arg Asn Ser Val Gly Gln His Ser His Ile Ser
1 5 10 15
Lys Arg Ala Leu Arg Pro Arg Ser Ser Pro Val Val Phe Ile Asn Thr
20 25 30
Thr Gln Glu Glu Ala Glu Gln Phe Gln Glu Ile Val Ala Ile Asp Asp
35 40 45
Ile Asp Tyr Leu Thr Gln Ala Ile Ala Leu Thr Ala Leu Phe Asn Gly
50 55 60
Ala Ile Asp Asn Thr Thr Gly Arg Phe Ile Pro Gly Lys Ala Arg Glu
65 70 75 80
Leu Ile Ala Asn Tyr Asn Glu Ser Leu Asp Ala Asn Ser Gln Pro Tyr
85 90 95
Lys Ile Gly Ile Phe Asn Thr His Gln Thr Thr Leu Thr Gln Gln Asn
100 105 110
Ser Ala Val Ser Ala Met Ile Ser Gln Ile Leu Glu Thr Leu Lys Arg
115 120 125
Val Met Gly Val Ala Leu Gly Ala Ser Ser Val Ala Gln Met Thr Ala
130 135 140
Ala Val Thr Asp Ala Phe Thr Asn Leu Asp Glu Gln Ser Gly Asp Ala
145 150 155 160
Trp Ile Phe Trp Glu Lys Lys Thr Ser Asn Lys Thr Thr Tyr Ser Tyr
165 170 175
Ala Ile Leu Phe Ala Phe Gln Asp Ser Thr Thr Gly Lys Leu Met Phe
180 185 190
Ala Leu Pro Met Ser Leu Glu Ile Glu Val Asp Val Ser Tyr Glu Arg
195 200 205
Val Leu Phe Ile Thr Val Glu Asp Lys Glu Thr Tyr Ser Val Lys Leu
210 215 220
Asp Thr Met Lys Val Gly Gln Leu Leu Phe Pro Lys Ser Pro Gly Ala
225 230 235 240
Asn Ala Leu Gln Ser Ala Arg Arg Leu Gly Thr Arg Ser Gly Ser Ala
245 250 255
Asp Leu Leu Ala Glu Pro Arg Pro Ile Thr Asp Ile Val Val Thr Asn
260 265 270
Trp Ala Lys Thr Lys Thr Phe Ala Thr Ala Ala Thr Gly Leu Tyr Thr
275 280 285
Asn Asp His Pro Leu Val Gln Val Met Ala Ser Glu Pro Asn Val Val
290 295 300
Asn Pro Leu Tyr Asp Gly Asn Gln Tyr Leu Val Ser Phe Ala Leu Asp
305 310 315 320
Gly Val Arg Gln Thr Leu Gly Phe Leu Leu Asn Cys Thr Leu Pro Asp
325 330 335
Gly Glu Leu Trp Phe Val Ser Ile
340
<210> 178
<211> 319
<212> БЕЛОК
<213> Bacillus sp.
<400> 178
Met Asn Lys Asn Gln Leu Lys Arg Pro Ile Val Ala Leu Met Leu Ala
1 5 10 15
Thr Ser Ile Gly Ile Pro Cys Val Ser Ala Pro Ser Ser Ala Phe Ala
20 25 30
Ala Glu Asn Thr Pro Ile Lys Val Asn Glu Asn Val Lys Val Gly Ile
35 40 45
Thr Asp Val Gln Ser Glu Leu Lys Lys Ile Gly Thr Tyr Tyr Tyr Thr
50 55 60
Asn Glu Ile Ala Gly Thr Lys Ile Glu Ser Trp Pro Thr Leu Thr Phe
65 70 75 80
Lys Ala Ser Pro Asp Ser Val Glu Thr Lys Val Asn Phe Ser Ile Thr
85 90 95
Gly Thr Thr Asn Lys Leu Asn Phe Asp Ser Asn Ile Val Glu Tyr Val
100 105 110
Gly Glu Asn Glu Phe Glu Asn Ile Thr Asp Gln Thr Gln Lys Phe Ser
115 120 125
Thr Ala Lys Tyr Thr Lys Ser Val Thr Glu Ser Ile Ser Thr Ala Thr
130 135 140
Thr Lys Gly Phe Asn Val Gly Gly Ser Ser Asn Ile Phe Thr Ile Pro
145 150 155 160
Leu Ile Leu Asn Asn Gly Ile Thr Val Asn Ala Gly Phe Asn Ser Ser
165 170 175
Thr Thr Glu Thr Gln Thr Lys Ala Glu Gln Lys Thr Ile Glu Ala Ser
180 185 190
Pro Gln Ser Ile Glu Val Pro Pro His Lys Lys Tyr Lys Ala Asp Val
195 200 205
Val Leu Glu Gln Arg Asn Phe Trp Gly Asp Ile Thr Phe Asn Gly Val
210 215 220
Gly Ser Asn Pro Met Thr Thr Ile Lys Gly Thr Ala Ser Tyr Trp Ala
225 230 235 240
Pro Asn Gly Met Gly Ala Trp Lys Gln Tyr Thr Phe Ser Asp Lys Thr
245 250 255
Lys Asn Tyr Trp Asn Lys Leu Thr Thr Ser Gln Lys Asn Ser Leu Asn
260 265 270
Gly Ile Lys Phe Glu Asn Asn Asp Val Lys Ala Glu Gly Thr Ala Lys
275 280 285
Ile Glu Gly Ile Phe Gly Ser Asn Leu Ile Val Arg Val Tyr Asp Ile
290 295 300
Thr Asp Lys Ser Asn Pro Lys Leu Val Glu Thr Lys Ser Phe Asn
305 310 315
<210> 179
<211> 432
<212> БЕЛОК
<213> Bacillus sp.
<400> 179
Leu Val Leu Ser Phe Tyr Ile Ala Thr Val Ile Leu Phe Arg Asn Lys
1 5 10 15
Ser Ile Ser Asn Val Gln Tyr Asn Phe Ile Phe Cys Asn Leu Val Gly
20 25 30
Ile Leu Pro Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys
35 40 45
Lys Val Met Ala Gly Leu Gly Val Gly Ala Met Ser Leu Ser Ile Trp
50 55 60
Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala Asn Asn Glu Tyr His Thr
65 70 75 80
Ile His Leu Ala Ala Asn Ala Asn Ile Ala Trp Asp Val Tyr Lys Gly
85 90 95
Trp Gln Ser Asp Asp Ala Gly Ile Val Leu Trp Asp Gly Ala Arg Gly
100 105 110
Asp Asn Glu Gln Phe Val Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala
115 120 125
Ile Val Asn Lys Asn Ser Gly Lys Pro Val Gly Ile Gly Ser Ala Ile
130 135 140
Ile Gly Ser Asp Gly Val Arg Asn Ile Pro Ser Phe Asn Asn Glu Gly
145 150 155 160
Leu Arg Gln Thr Ser Trp Thr Gly Ala Pro Asn Glu Gln Trp Tyr Leu
165 170 175
Arg Asp Lys Gly Asn Asn Asn Phe Glu Ile Val Asn Gln Gly Ser Gly
180 185 190
Lys Val Ala Ser Trp Ala Gly Val Glu Met Phe Gly Tyr Lys Asp Tyr
195 200 205
Val Asp Leu Asp Glu Ser Asn Pro Ser Asp Asn Asp Arg Leu Phe Asn
210 215 220
Ile Pro Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu Pro Thr Val
225 230 235 240
Gly Thr Arg Pro Asn Ala Pro Glu Tyr Asn Thr Ser Gly Ala Ile Asp
245 250 255
Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu
260 265 270
Ile Pro Ser Ile Met Val Lys Asp Asn Gly Ala Ser Asp Tyr Thr Lys
275 280 285
Ile His Asn Ser Pro Tyr Tyr Val Leu Val Lys Glu Glu Tyr Trp Glu
290 295 300
Lys Val Arg Ser Glu Ile Ile Pro Ala Gly Ala Thr Ser Lys Tyr Thr
305 310 315 320
Val Lys Ser Gly Val Ser Glu Ile Asp Gln Lys Lys Met Ser Asp Thr
325 330 335
Leu Ala Met Ser Phe Gly Ser Asp Leu Gly Leu Lys Phe Gly Glu Ser
340 345 350
Ser Leu Ser Ile Lys Ser Ser Val Ser Arg Thr Ile Gln Thr Glu Val
355 360 365
Thr Lys Thr Ser Thr Asn Ala Thr Glu Glu Thr Glu Glu Lys Ser Ile
370 375 380
Pro Ser Ile Pro Gly Gln Asn Thr Gly Phe Thr Ala Tyr Gln Leu Val
385 390 395 400
Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly Ser Val Val Thr Asp Pro
405 410 415
Ser Ile Val Arg Asp Ser Lys Gln Thr Ile Ala Arg Thr Ile Ala Lys
420 425 430
<210> 180
<211> 204
<212> БЕЛОК
<213> Неизвестный
<220>
<223> неизвестный организм из образца окружающей среды
<400> 180
Met Gly Asn Asn His Phe His Asn Val Phe Ala Val Lys Gln Gln His
1 5 10 15
Leu Gly Gln Val Leu Lys Leu Lys Thr Phe Phe Glu Glu Leu Asp Thr
20 25 30
Asp Phe Asn Asp Phe Ala Asp Pro Arg Thr Leu Val Asn Val Leu Glu
35 40 45
Ser Asn Arg Asp Ile Asn Phe Leu Asp Ser Ser Gln Glu Ala Ile Ile
50 55 60
Gly Gly Glu Ala Leu Gln Ile Ser Glu Leu Ile Glu Lys Ile Ser Asn
65 70 75 80
Leu Ile Ile Lys Asp Ile Leu Ala Ile Pro Thr Tyr Arg Trp Gly Glu
85 90 95
Ser Ile Asn Gln Asp Ile Val Ser Gln Val Thr Asp Ala Ile Thr Asn
100 105 110
Met Phe Ile Asn Leu Asn Lys Gln Asp Ala Glu Pro Trp Leu Phe Trp
115 120 125
Tyr Asn Thr Glu Ser Val Leu Pro Arg Tyr Thr Tyr Asn Ile Leu Ile
130 135 140
Ala Ile Asp Thr Gln Asp Lys Tyr Tyr Leu Thr Asp Val Leu Ala Ile
145 150 155 160
Ser Phe Asp Val Leu Val Val Lys Lys Thr Lys Gln Glu Val Cys Ser
165 170 175
Phe Thr Val Asn Asp Ser Ala Gln Cys Leu Ile Met Met Lys His Met
180 185 190
Leu Val Gly Ile Lys Phe Glu Asp Val Tyr Pro Ser
195 200
<210> 181
<211> 407
<212> БЕЛОК
<213> Bacillus sp.
<400> 181
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ala Ser Gln Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Ser Leu His Leu Thr Asn Asn Thr Glu Lys Val
35 40 45
Trp Asp Val Tyr Gly Gly Trp Ala Ser Asn Asp Ala Gly Ile Leu Leu
50 55 60
Tyr Asn Ala Asn Gly Lys Asp Asn Gln Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Leu Gly Gly Gly Ile Asn Tyr Ser Asp Gly Val Arg Asn Met Ser
100 105 110
Asn Tyr Asn Gly Asn Pro Ala Asp Ile Leu Gly Gln Asn Asp Trp Thr
115 120 125
Gly Ala Asp Thr Asp Lys Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn
130 135 140
Tyr Glu Ile Val Asn Gln Gly Lys Gly Lys Val Ala Ser Tyr Ala Ala
145 150 155 160
Thr Asn Ile Ala Pro Tyr Leu His Tyr Val Asp Leu Asp Glu Ser Asn
165 170 175
Pro Ser Asp Gly Asp Arg Leu Phe Arg Ile Gln Lys Asn Val Leu Pro
180 185 190
Leu Pro Asp Gly Leu Gln Leu Pro Gly Phe Ser Asp Tyr Gly Thr Phe
195 200 205
Ser Leu Pro Lys Leu Pro Ala Thr Gly Asn Arg Pro Asp Ala Pro Asn
210 215 220
Tyr Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val
225 230 235 240
Val Val Gly Ala Ser Leu Ile Pro Cys Phe Met Val Lys Asp Ser Gln
245 250 255
Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Ile
260 265 270
Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly
275 280 285
Leu Thr Arg Asn Tyr Thr Phe Lys Thr Gly Met Thr Ser Val Asp Gln
290 295 300
Gln Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly
305 310 315 320
Leu Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg
325 330 335
Thr Ile Gln Thr Glu Ile Thr Ser Thr Asn Thr Glu Ala Ser Glu Glu
340 345 350
Thr Ile Ser Ser Thr Val Thr Ser Glu Pro Gly Lys Ser Gln Thr Gly
355 360 365
Phe Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp
370 375 380
Gly Ser Ile Val Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr
385 390 395 400
Ile Ala Arg Lys Lys Ser Glu
405
<210> 182
<211> 455
<212> БЕЛОК
<213> Bacillus sp.
<400> 182
Met Cys Trp Ile Leu Ser Ile Ile Val Arg Glu Asn Ala Ser Ser Gly
1 5 10 15
Tyr Leu Arg Thr Lys Tyr Ser Trp Gln Asn Cys Cys Thr Ser Ile Asp
20 25 30
Asn Ser Thr Ile Leu Lys Asn Lys Gly Met Asn Leu Met Lys Thr Tyr
35 40 45
Lys Arg Asn Asp Glu Leu Met Asn Gln Val Ser Thr Lys Asn Met Asp
50 55 60
Met Asn Leu Lys Asn Lys Lys Asp Val Arg Ser Cys Asn Cys Asp Pro
65 70 75 80
Val Gly Tyr Asn Thr Pro Asn Leu Pro Lys Glu Ser Lys Ser Phe Gln
85 90 95
Thr Ile Thr Ser Phe Tyr Ser Asp Asn Thr Ser Ser Phe Tyr Ala Arg
100 105 110
Ser Asn Lys Ser Gly Glu Val Ile Gly Pro Gly Glu Cys Gly Thr Leu
115 120 125
Asn Leu Gly Asn Gln Val Val Cys Asp Ala Tyr Thr Lys Lys Met Asp
130 135 140
Ala Asn Leu Phe Val Phe Tyr Gln Leu Asp Asn Gly Tyr Phe Thr Ile
145 150 155 160
Ala Asn Arg Glu Thr Gly Glu Val Leu Glu Tyr Met Tyr Glu Asn Asp
165 170 175
Asp Lys Glu Glu Ala Ser Ile Val Phe Arg Pro Tyr Thr Gly Glu Ala
180 185 190
Ser Gln Val Ile Lys Lys Ile Asp Ser Glu Asn Phe Pro Thr Phe Ile
195 200 205
Leu Leu Val Glu Asn Arg Ser Lys Leu Ile Gly Leu Cys Asn Glu Lys
210 215 220
Leu Asp Gln Lys Thr Lys Val Thr Thr Arg Phe Ser His Ile Thr Ser
225 230 235 240
Glu Ala Thr Val Phe Thr His Val Glu Lys Asp Thr Val Asp Ile Thr
245 250 255
Ile Pro Ala Leu Pro Pro Thr Thr Glu Leu Gly Pro Val Pro Glu Leu
260 265 270
Lys Gly Phe Asn Asp Ile Leu Pro Glu Asn Asp Asp Glu Glu Arg Val
275 280 285
Lys Arg Ala Ile Ile Gly Ser Thr Leu Ile Pro Cys Val Leu Val Asn
290 295 300
Asp Ser Ile Leu Leu Ala Asp Arg Ile Lys Glu His Pro Tyr Tyr Thr
305 310 315 320
Leu Glu Tyr Arg Gln Tyr Trp His Leu Leu Trp Ser Asp Leu Leu Thr
325 330 335
Ala Gly Ser Ser Gln Lys Lys Thr Glu Val Thr Gly Ile Ser Asn Gly
340 345 350
Ala Gln Glu Asn Met Lys Gln Leu Ile Ser Met Ser Ile Gly Thr Asp
355 360 365
Leu Gly Leu Lys Cys Thr Gly Lys Ser Ala Leu Phe Lys Glu Gln Ile
370 375 380
Leu Ser Arg Leu Asp Ile Lys Leu Ser Tyr Asn Thr Asp Leu Glu Glu
385 390 395 400
Thr Thr Lys Met Val Asp Val Glu Asn Pro Asn Asp Phe Thr Val Arg
405 410 415
Tyr Ala Lys Tyr Ala Lys Ala His Glu Phe Ile Leu Arg Arg Ser Asp
420 425 430
Gly Thr Val Val Asp Ser Pro Trp Val Val Val Asp Glu Asn Lys Met
435 440 445
Tyr Leu Gln Arg Tyr Lys Lys
450 455
<210> 183
<211> 252
<212> БЕЛОК
<213> Aeromonas sp.
<400> 183
Met Asn Thr Thr Val Asp Lys Ala Lys Asn Val Val Ser Arg Asn Ile
1 5 10 15
Phe Val Ser Leu Ser Ala Ser Glu Gly Glu Val Asn Phe Asn Glu Ile
20 25 30
Phe Ser Ile Thr Asp Pro Asn Ile Ala Pro Gln Ala Phe Ala Met Ala
35 40 45
Gln Val Phe Gln Gln Ala Leu Val Pro Thr Pro Asp Lys Lys Ser Leu
50 55 60
Arg Phe Asp Val Thr Lys Ala Ile Thr Leu Ile Lys Gln His Pro Glu
65 70 75 80
Met Ala Val Ile Asn Phe Thr Asp Gln Val Val Glu Gln Ser Asn Ser
85 90 95
Ala Val Thr Val Met Val Asp Lys Val Leu Asp Ile Leu Asn Lys Val
100 105 110
Leu Gly Val Val Leu Gly Ala Ala Pro Lys Ala Lys Leu Val Glu Ala
115 120 125
Ile Ser Ser Ala Phe Thr Asn Leu Asn Glu Gln Lys Asp Ser Ala Trp
130 135 140
Ile Phe Trp Glu Lys Asn Glu Ala His Lys Thr Thr Tyr Gln Tyr Asn
145 150 155 160
Ile Leu Phe Gly Val Gln Ser Lys Asp Thr Gly Leu Phe Leu Tyr Gly
165 170 175
Leu Pro Met Gly Met Glu Ile Val Val Asp Ile Asp Lys Glu Arg Val
180 185 190
Leu Phe Ile Thr Leu Lys Asp Lys Glu Ser Tyr Arg Val His Leu Gln
195 200 205
Ser Leu Ser Val Val Glu Met Leu Lys Ala Pro Ser Asn Leu Lys Leu
210 215 220
Ala Ala Arg Gly Gly Lys Asp Pro His Gln Glu Ala Gln Glu Phe Phe
225 230 235 240
Ala Gln Gln Lys Lys Lys Glu Pro Val Arg Ile Lys
245 250
<210> 184
<211> 420
<212> БЕЛОК
<213> Bacillus sp.
<400> 184
Met Gly Tyr Thr Asp Lys Ser Thr Asn Glu Thr Gln Asn Ala Thr Gln
1 5 10 15
Gln Lys Thr Thr Gly Ser Tyr Tyr Asp Asn Glu Pro Lys Leu Asn Pro
20 25 30
Leu Ala Gln Ile Asp Lys Trp Thr Gln Asp Leu Ser Lys Asn Thr Gly
35 40 45
Ala Ala Asn Tyr Lys Thr Thr Leu Gly Leu Val Glu Lys Thr Leu Pro
50 55 60
Thr Ile Tyr Lys Asp Met Thr Leu Gly Asn Phe Asn Asn Thr Ala Arg
65 70 75 80
Val Leu Thr Met Tyr Gly Ala Ala Leu Ile Pro Tyr Gly Gly Val Phe
85 90 95
Ile Ser Pro Ile Ile Gly Ile Leu Trp Pro Glu Asn Thr Thr Ala Gln
100 105 110
Asp Ser Lys Val Ala Lys Met Val Lys Asp Leu Thr Ser Leu Met Asp
115 120 125
Lys Lys Ile Ser Asp Tyr Asp Leu Ala Lys Leu Gln Gln Lys Thr Asp
130 135 140
Ala Leu Met Ile Val Leu Lys Lys Phe Glu Asp Ser Val Asn Asn Lys
145 150 155 160
Pro Gln Ser Glu Ser Tyr Tyr Asp Ser Ile Gln Asn Ala Asn Ile Asn
165 170 175
Tyr Ala Asp Ile Leu Asn Thr Lys Phe Asp Glu Leu Ile Lys Glu Ser
180 185 190
Gln Thr Lys Gly Tyr Gln Leu Ala Glu Leu Pro Ile Phe Thr Ile Ile
195 200 205
Ala Thr Ala His Leu Gln Phe Leu Arg Leu Ile Glu Ala Ser Val Gln
210 215 220
Asn Pro Arg Ile Lys Met Asp Pro Glu His Leu Ser Thr Tyr Phe Pro
225 230 235 240
Lys Ala Lys Lys Lys Ala Glu Asp Tyr Arg Asn Tyr Val Asn Glu Thr
245 250 255
Phe Tyr Gly Ala Leu Glu Glu Phe His Arg Gln Thr Gly Gly Gln Leu
260 265 270
Lys Ser Asp Glu Glu Ile Glu Ala Phe Gln Lys Ile Val Asn Ile Gly
275 280 285
Lys Ile Thr Ala His Asn Glu Ala Phe Leu Phe Glu Ala Ser Asp Ala
290 295 300
Ser Asp Gly Asn Trp Glu Lys Arg Ser Gly Lys Trp Tyr Phe Ile Gly
305 310 315 320
Lys Asp Lys Ile Ala Lys Thr Gly Trp Ile Leu Leu Ile Asn Ile Trp
325 330 335
Tyr Tyr Leu Ser Pro Ala Asp Gly Thr Lys Asn Ser Ala Gly Val Val
340 345 350
Phe Asn Lys Gly Glu Met Met Thr Gly Trp Val Glu Ile Lys Asp Thr
355 360 365
Lys Thr Gly Val Lys Tyr Trp Tyr Tyr Phe Ser Pro Ala Asp Gly Phe
370 375 380
Thr Gly Ile Gly Gly Asn Thr Phe Ser Glu Gly Glu Met Val Thr Gly
385 390 395 400
Ser Val Tyr Ile Gly Asn Lys Thr Tyr Asn Phe Ser Lys Asn Gly Val
405 410 415
Cys Gln Asn Pro
420
<210> 185
<211> 341
<212> БЕЛОК
<213> Bacillus sp.
<400> 185
Val Thr Asn Gly Leu Asp Ser Asn Thr Gln Asp Ser Asn Glu Leu Asp
1 5 10 15
Asn Gly Val Leu Ala Leu Asp Pro Phe Thr Phe Tyr His Ile Arg Thr
20 25 30
Phe Cys Gly Lys Thr Leu Asp Val Thr Asn Ser Ser Ile Ala Asp His
35 40 45
Ala Met Ile Ile Gln His His Ser Asn Asn Gln Leu Asn Gln Gln Phe
50 55 60
Leu Ile Phe Thr Leu Asp Asp Gly Tyr Ser Ile Ile Thr Ser Val Ile
65 70 75 80
Ser Gly Lys Val Leu Asp Ile Ser Gln Ser Gly Ser Leu Lys Gly His
85 90 95
Val Ile Gln His Tyr Leu Asn His Gly Asn His Gln Lys Phe Leu Val
100 105 110
Gly Asn Asn Gly Thr Ile Ala Ala Lys His Ser Gly Lys Val Phe Asp
115 120 125
Val Tyr Lys Ala Ser Thr Ala Asn Asn Val Pro Ile Lys Glu Asp Asn
130 135 140
Pro His Asn Gly Ala Asn Gln Lys Phe Thr Phe Glu Arg Val Arg Thr
145 150 155 160
Phe Thr Val Pro Ser Pro Ser Lys Gly Thr Leu Pro Pro Ala Pro Asp
165 170 175
Phe Lys Asn Asp Ile Asn Glu Val Leu Pro Asp Lys Thr Asn Pro Val
180 185 190
Ile Thr His Asp Thr Asn Ile Pro Cys Ile Met Ile Asp Asp Lys Thr
195 200 205
Phe Ser Ser Pro Gln Lys Ile Gln Tyr Ser Pro Tyr Tyr Lys Leu Val
210 215 220
Lys Ile Gln Tyr Trp Glu Lys Val Thr Gln Arg Ile Leu Gly Pro Arg
225 230 235 240
Asp Thr Tyr Ser Tyr Gln Lys Ser Lys Gly Val Ser Arg Thr Asp Gln
245 250 255
Ser Ser Met Thr Glu Thr Val Ser Met Ser Ile Gly Ala Asp Phe Gly
260 265 270
Phe Met Phe Lys Gly Phe Ser Ala Ser Leu Ser Thr Arg Val Thr Lys
275 280 285
Glu Leu Ser Val Thr Lys Ser Thr Ser Tyr Thr Glu Met Thr Glu Glu
290 295 300
Thr Tyr Thr Glu Ser Tyr Thr Asn Pro Phe Asn Tyr Ala Leu Ala Arg
305 310 315 320
Ala Gln Tyr Met Leu Val Asn Glu Phe Tyr Val Thr Arg Met Gly Trp
325 330 335
Tyr Thr Tyr Asn Lys
340
<210> 186
<211> 781
<212> БЕЛОК
<213> Bacillus sp.
<400> 186
Val Met Asn Met Ser Ser Asn Thr Met Val Pro Tyr Asn Val Leu Arg
1 5 10 15
Asn Leu Asp Met Pro Thr Ile Ser Gly Thr Arg Trp Asn Lys Glu Met
20 25 30
Phe Thr Asn Ala Leu Asp Asn Thr Ser Ser Leu Leu Thr Leu Ile Glu
35 40 45
Lys Gly Ile Asn Asp Gly Asp Asp Val Leu Gly Leu Leu Ser Phe Ile
50 55 60
Gly Leu Thr Ala Leu Glu Ala Ile Pro Val Val Gly Gly Val Leu Ser
65 70 75 80
Ser Ile Val Ser Met Leu Phe Phe Pro Thr Lys Ser Ser Ile Asp Phe
85 90 95
Lys Lys Ile Trp Glu His Leu Glu Lys Ala Ile Asn Gln Ile Val Asp
100 105 110
Lys Lys Ile Glu Glu Ala Leu Met Ser Gln Leu Met Gln Glu Ile Ala
115 120 125
Gly Leu Ala Asn Val Leu Glu Glu Tyr Arg Asp Ala Tyr Asp Ser Tyr
130 135 140
Asn Gly Lys Lys Val Phe Asp Ile Gln Asp Arg Leu Thr Pro Glu Glu
145 150 155 160
Tyr Leu Val Gln Val Phe Thr Ser Ala Asn Leu Leu Phe Ile Gln Arg
165 170 175
Ile Pro Thr Phe Gln Asn Pro Lys Tyr Asp Val Leu Phe Leu Pro Phe
180 185 190
Phe Val His Val Ala Glu Met His Ile Leu Leu Ile Arg Asp Ala Ala
195 200 205
Ile His Gly Leu Glu Trp Gly Met Asp Glu Glu Ile His Gln Lys Phe
210 215 220
Lys Arg Asp Phe Lys Lys Ile Ile Asp Lys Tyr Thr Asn His Leu Leu
225 230 235 240
Asn Thr Tyr Lys Lys Gly Leu Lys Glu Val Ser Glu Arg Glu Leu Asn
245 250 255
Asp Lys Asp Phe Pro Thr Thr Asn Asn Lys Tyr His Tyr Ile Asn Thr
260 265 270
Val Arg Trp Asn Val Ile Asn Gln Tyr Lys Arg Gly Met Thr Leu Thr
275 280 285
Val Phe Asp Phe Ala Tyr Lys Trp Lys Tyr Tyr Gln Glu Ile Tyr Gln
290 295 300
Asn Asn Ile Glu Leu Asn Pro Phe Arg Thr Ile Tyr Ser Asp Ile Ala
305 310 315 320
Gly Ser Val Tyr Pro Tyr Glu Lys Ala Thr Asn Glu Ile Asp Thr Ile
325 330 335
Ile Lys Asp Asn Asn Leu Lys Tyr Arg Gly Ile Phe Lys Arg Leu Gln
340 345 350
Val Asn Tyr Ala Glu Arg Ile Asp Ser Ile Gln Ser Ser Tyr Ile Gln
355 360 365
Asn Asn Glu Ile Ile Asp Ser Glu Arg Thr Gly Gly Thr Gly Gly Arg
370 375 380
Glu Ser Val Leu Asp Ile Glu Ser Pro Val Lys Asn Pro Leu Met Gln
385 390 395 400
Val Asn Met Trp Ser Glu Leu Val Pro Ile Ser Leu Gly Phe Lys Phe
405 410 415
Tyr Asn Glu Lys Glu Lys Asn Ile Gly Gly Gly Ser Trp Trp Pro Asn
420 425 430
Pro His Lys Phe Gly Ala Tyr Gln Tyr Val Gly Asn Lys Val Ser Ser
435 440 445
Ile Met Gly Phe Gly Lys Asn Ala Thr Gly Gly Phe Asn Ser Leu Asp
450 455 460
Ala Met Val Val Gly Phe Lys Arg Asp Asp Tyr Thr Pro Glu Asn Arg
465 470 475 480
Phe Val Gly Val Ser Lys Asn Asp Glu Pro Ile Thr Lys Val Ile Asp
485 490 495
Val Gly Asn Tyr His Gln Gly Lys Phe Glu Ser Asn Ile Thr Met Ile
500 505 510
Asp Glu Pro Met Phe Gly Asp Gly Val Leu Gln Leu Glu Asn Tyr Ser
515 520 525
Asn Asn Leu Asn Gln Asp Ser Tyr Val Thr Tyr Gln Ile His Thr Glu
530 535 540
Thr Ala Gly Thr Tyr Glu Leu His Ala Val Ile Gly Ala Glu Lys Gln
545 550 555 560
Lys Asp Lys Val Val Leu Lys Val Ser Ile Asn Gly Asn His Ser Glu
565 570 575
Asn Ile Thr Thr Asp Phe Phe Asp Asn Gly Asp Ile Trp Asp Gly Met
580 585 590
Ser Leu Asn Asp Gly Phe Val Tyr Lys Arg Val Leu Ile Gly Arg Phe
595 600 605
Gln Phe Lys Lys Gly Leu Asn Asn Ile Thr Ile Tyr Asn Gly Val Leu
610 615 620
Gln Thr Ser Ala Ser Ile Lys Ile Trp Asn Leu Ala Ala Leu Glu Leu
625 630 635 640
Thr Ile Pro Asp Asn Thr Glu Trp Lys Phe Gln Asn Gly Asp Phe Ser
645 650 655
Lys Gly Leu Lys Gly Trp Thr Val Glu Glu Gly Ser Pro Ser Ala Gly
660 665 670
Asn Gln Tyr Leu Asp Met His Gln Asn Ser Lys Val Ser Gln Thr Ile
675 680 685
Lys Val Asn Pro Asn Thr Thr Tyr Glu Leu Ser Tyr Asp Ser Ile Thr
690 695 700
Pro Ser Ser Asn Asn Phe Phe Gly Tyr Gly Thr Leu Tyr Leu Asp Phe
705 710 715 720
Leu Ser Met Asp Gly Ser Lys Gln Ser Gly Val Ala His Pro Ile Glu
725 730 735
Gln Gln Gly Asn Ser Trp Ala Arg Lys Thr Tyr Lys Phe Thr Thr Pro
740 745 750
Ser Asp Ile Lys Lys Leu Lys Leu Thr Ile Ala Asn Glu Ala Ser Asn
755 760 765
Gln Gly Phe Asn Ile Asp Asn Ile Ala Val Thr Glu Gly
770 775 780
<210> 187
<211> 322
<212> БЕЛОК
<213> Bacillus sp.
<400> 187
Met Tyr Ile Leu Leu Leu His Phe Arg Lys Lys Trp Tyr Ile Ile Lys
1 5 10 15
Ser Gln Lys Phe Lys Met Asn Gln Asp Glu Lys Ile Gln Arg Glu Tyr
20 25 30
Phe Pro Glu Asn Asp Pro Leu Val Gly Gly Ile Ala Gly Thr Ile Gly
35 40 45
Thr Met Gly Gly Val Ile Gly Gly Ala Ala Leu Ala Ser Arg Val Asp
50 55 60
Tyr Gly Leu Lys Ser Gln Leu Asn Gln Val Asn Ile Thr Ser Val Gly
65 70 75 80
Thr Asp Phe Lys Val Thr Pro Ser Lys Val Val Glu Glu Asp Gln Gly
85 90 95
Val Ile Asn Leu Leu Ser Tyr Arg Asn Asn Thr Pro Glu Lys Gln Lys
100 105 110
Ala Phe Thr Thr Glu Glu Ser Tyr Lys Lys Val Asp Thr Val Thr Ala
115 120 125
Thr Asn Gln Tyr Gly Phe Lys Leu Ser Ala Lys Ser Val Thr Lys Phe
130 135 140
Gly Phe Asp Ile Gly Leu Ala Lys Gly Glu Gln Thr Phe Glu Val Ser
145 150 155 160
Ala Glu Phe Ala Phe Asn Arg Thr Asp Thr Phe Thr Thr Thr Asn Glu
165 170 175
Lys Asn Val Thr Phe Lys Ala Gln Glu Val Ile Ala Ala Pro Gly Gly
180 185 190
Thr Thr Asp Tyr Phe Ser Arg Val Lys Lys Ala Lys Phe Ser Gly Ser
195 200 205
Phe Gln Thr Asp Ala Ala Leu Asp Ser Leu Lys Val Lys Leu Pro Ile
210 215 220
Val Lys Gly Gly Asn Met Asn Val Thr His Thr Glu Glu Val Thr Leu
225 230 235 240
Asn Ser Glu Asp Leu Tyr Asn Ile Phe Lys Leu Thr Ser Lys Asp Phe
245 250 255
Pro Leu Pro Ser Tyr Leu Ser Phe Asp Asp Thr Asn Lys Ser Ile Leu
260 265 270
Val Lys Asn Ala Thr Phe Asn Tyr Thr Gly Glu Ile Gly Tyr His Thr
275 280 285
Ala Ile Asp Ala Lys Phe Thr Pro Phe Asp Gln Thr Lys Ala Thr Ala
290 295 300
Val Met Pro Tyr Ala Lys Tyr Ala Glu Lys Thr Gln Ala Asn Ala Leu
305 310 315 320
Leu Arg
<210> 188
<211> 389
<212> БЕЛОК
<213> Bacillus sp.
<400> 188
Met Lys Ser Leu Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ala Ser Gln Ala Glu Thr Pro Glu
20 25 30
Lys Asn Lys Tyr Tyr Ser Ile Tyr Ser Lys Val Asn Ser Thr Leu Ala
35 40 45
Trp Thr Pro Tyr Ser Ser Ser Tyr Asp Gly Ala Ile Thr Asn Val Ile
50 55 60
Lys Leu Asn Met Glu Asp Asn Gln Lys Tyr Leu Phe Phe Pro Leu Asn
65 70 75 80
Gly Gly Ser Tyr Ala Ile Val Asn Lys Ser Ser Gly Lys Val Ala Ser
85 90 95
Ile His Thr Asn Leu Val Val Pro Arg Val Gly Ser Phe Ser Cys Asn
100 105 110
Gly Ser Ser Gly Gln Lys Trp Asn Leu Arg Asp Lys Gly Asn Gly Tyr
115 120 125
Tyr Glu Phe Met Asn Gln Gly Asn Gly Lys Val Ala Ala Thr Thr Leu
130 135 140
Asn Ser Ser Gly Ala Ile Gln Asp Leu Ser Val Gly Phe Gly Asn Ser
145 150 155 160
Asn Pro Ser Asp Pro Glu Lys Leu Phe Ser Phe Ser Ser Pro Ile Ala
165 170 175
Ser Ile Met Tyr Gln Thr Leu Asn Met Glu Glu Asn Pro Thr Phe Ser
180 185 190
Leu Pro Asn Leu Pro Asn Thr Gly Thr Arg Pro Ala Ala Pro Glu Tyr
195 200 205
Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val
210 215 220
Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Asn Asp Asn Ser Val
225 230 235 240
Asp Glu Tyr Thr Lys Leu His Asn Ser Pro Tyr Tyr Thr Leu Ile Lys
245 250 255
Glu Glu Phe Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ser Gly Leu
260 265 270
Thr Arg Asn Tyr Ala Phe Lys Thr Gly Met Thr Thr Val Asp Gln Gln
275 280 285
Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Leu Gly Leu
290 295 300
Lys Phe Gly Asp Leu Thr Ala Ala Val Lys Ala Glu Ile Ser Arg Thr
305 310 315 320
Ile Gln Thr Glu Ile Ser Thr Thr Asn Thr Glu Ala Ser Glu Glu Thr
325 330 335
Ile Thr Ser Thr Val Ala Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr
340 345 350
Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser
355 360 365
Leu Val Ser Glu Pro Trp Ile Val Lys Asn Asn Lys Ile Thr Ile Ala
370 375 380
Arg Lys Asn Ala Gln
385
<210> 189
<211> 372
<212> БЕЛОК
<213> Bacillus sp.
<400> 189
Met Thr Asn Glu Leu Asn Gly Lys Met Gln Glu Ser Lys Glu Leu Asp
1 5 10 15
Ser Gly Ile Leu Ala Leu Asp Pro Phe Thr Phe Tyr Arg Ile Arg Thr
20 25 30
Phe Cys Gly Lys Met Phe Asp Val Ser Gly Gln Ser Ile Ala Asn Glu
35 40 45
Ala Arg Val Val Gln Tyr Ser Thr Asn Asn Lys Lys Asn Gln Asn Phe
50 55 60
Leu Ile Phe Thr Leu Asp Asp Asn Tyr Ser Ile Ile Ala Ala Glu His
65 70 75 80
Ser Gly Lys Val Leu Asp Ile Ala Asp Thr Gly Thr Ile Lys Gly Arg
85 90 95
Leu Ile Gln Tyr Asn Phe Ser Asn Gly Thr Asn Gln Gln Phe Leu Ile
100 105 110
Asn Asn Asn Gly Thr Ile Ala Ile Lys Arg Ser Gly Lys Val Leu Asp
115 120 125
Val Ala Asn Gly Ser Thr Ala Asn Glu Val Pro Ile Ile Glu Tyr Asn
130 135 140
Phe Gly Asn Ala Ala Asn Gln Lys Phe Thr Phe Glu Arg Val Arg Asn
145 150 155 160
Phe Gln Val Pro Ala Pro Ser Thr Gly Thr Leu Pro Thr Ala Pro Asp
165 170 175
Phe Arg Asn Asp Ile Asn Glu Gln Leu Pro Asp Lys Thr Asn Pro Ile
180 185 190
Ile Thr His Tyr Thr Asn Val Pro Tyr Ile Ile Val Gly Asp Lys Thr
195 200 205
Phe Asp Ser Gln Gln Lys Ile Gln Ser Ser Pro Tyr Tyr Lys Leu Val
210 215 220
Lys Ile Gln Tyr Trp Glu Lys Val Thr Gln Arg Ile Leu Gly Pro Arg
225 230 235 240
Asp Thr Tyr Ser Tyr Gln Lys Lys Thr Gly Ile Ser Arg Thr Asp Gln
245 250 255
Val Ser Met Thr Glu Met Val Ser Met Ser Val Gly Ala Asp Phe Gly
260 265 270
Phe Met Phe Lys Gly Phe Ser Ala Ser Leu Ser Thr Lys Ile Thr Lys
275 280 285
Glu Leu Ser Val Thr Lys Ser Thr Ser Thr Thr Glu Met Thr Glu Gln
290 295 300
Ile His Thr Glu Ser Tyr Thr Asn Pro Phe Asn His Glu Leu Ala Arg
305 310 315 320
Ala Gln Tyr Met Leu Val Asn Glu Phe Tyr Val Thr Arg Met Asp Gly
325 330 335
Thr Arg Ile Thr Ala Asn Trp Thr Leu Arg Asp Asn Thr Gln Thr Val
340 345 350
Thr Arg Ile Phe Pro Lys Ser Lys Ser Ser Thr Asp Gly Val Gly Glu
355 360 365
Ser Thr Ser Leu
370
<210> 190
<211> 338
<212> БЕЛОК
<213> Bacillus sp.
<400> 190
Met Thr Asp Val Ile Pro Asp Ile Asn Tyr Val Leu Asn Leu Lys Ala
1 5 10 15
Asn Ser Tyr Leu Arg Trp Asp Val Ala Tyr Glu Ser Leu Asp Glu Asn
20 25 30
Ala Gln Ile Ile Leu Tyr Gly Gln Ser Thr Lys Ala Asn Gln Arg Phe
35 40 45
Ile Phe Met Ser Leu Asp Asn Gln Leu Tyr Ala Ile Val Ser Ala Asn
50 55 60
Ser Gly Lys Val Val Tyr Thr Asn Gly Asp Lys Phe Tyr Gln Ala Ser
65 70 75 80
Trp Asn Gly Asp Asp Ser Gln Lys Trp Ser Leu Thr Asn Thr Ser Ala
85 90 95
Asn Tyr Phe Glu Ile Lys Ser Gln Lys Tyr Gly Lys Val Ala Ser Tyr
100 105 110
Gly Ser Gly Gly Gly Glu Tyr Val Asp Leu Asp Tyr Pro Asn Ala Gly
115 120 125
Asp Asn Asp Arg Leu Phe Gln Leu Ile Phe Asp Gln Pro Ala Asn Ser
130 135 140
Leu Ser Lys Pro Ser Leu Pro Val Pro Gln Asn Arg Pro Ser Val Pro
145 150 155 160
Thr Tyr Thr Ser Gly Pro Tyr Glu Thr Met Pro Asp Arg Ser Glu Ser
165 170 175
Arg Val Val Gly Ala Ser His Ile Pro Tyr Phe Met Val Asn Asp Gly
180 185 190
Ser Arg Ser Asp Asn Ile Lys Leu Asn Ser Ser Pro Tyr Tyr Lys Leu
195 200 205
Leu Lys Glu Val Tyr Trp Glu Lys Val Phe Ser Thr Val Val Ala Pro
210 215 220
Asn Gln Gln Gln Gln Tyr Ser Tyr Thr Thr Gly Val Ser Ser Glu Asp
225 230 235 240
Gln Lys Thr Met Ser Asp Thr Leu Gln Met Thr Phe Gly Leu Asp Leu
245 250 255
Gly Leu Asn Phe Gln Lys Ala Thr Ala Ala Leu Ser Ala Ser Ile Thr
260 265 270
Arg Thr Leu Asn Thr Thr Ile Ser Ser Thr Asn Ser Asn Ser Thr Ser
275 280 285
Thr Ser Ile Ser Ser Val Val Ser Gly Thr Ala Gly Tyr Val Thr Gly
290 295 300
Tyr Thr Ile Tyr Gln Leu Val Ser Lys Tyr Thr Leu Tyr Arg Ala Asp
305 310 315 320
Gly Thr Ser Val Ser Asn Pro Val Ile Thr Arg Ser Pro Asn Glu Ile
325 330 335
Val Asn
<210> 191
<211> 270
<212> БЕЛОК
<213> Bacillus sp.
<400> 191
Val Asp Thr Ser Arg Leu Thr Val Glu Asn Ala Glu Ser Tyr Gly Phe
1 5 10 15
Glu Ile Lys Glu Ser Lys Pro Gln Gly Thr Met Phe Met Gly Glu Ser
20 25 30
Thr Leu Asn Asn Arg Thr Asp Glu Thr Gln Thr Leu Asn Ser Asp Ser
35 40 45
Phe Thr Lys Thr Leu Thr Asp Ser Ile Thr Leu Ser Val Thr Asn Gly
50 55 60
Ile Thr Thr Thr Val Asn Ile Ser Ile Ser Gly Lys Ile Phe Gly Met
65 70 75 80
Gly Ala Glu Thr Ser Met Ser Phe Glu Val Ser Thr Ser Thr Thr Asn
85 90 95
Glu Gln Thr Asn Glu Glu Ser Val Ala Tyr Thr Val Pro Ser Gln Thr
100 105 110
Ile Val Val Pro Ala Gln Thr Thr Tyr Tyr Val His Thr Leu Leu Glu
115 120 125
Arg Asn Gln Leu Glu Gly Ile Cys Arg Leu Arg Ala Asp Leu Thr Gly
130 135 140
Phe Phe Phe Val Leu Thr Lys Phe Ala Gly Lys Glu Val Pro Cys Tyr
145 150 155 160
Phe Gly Glu Ile Tyr Asp Phe Val Asn Gln Tyr Gln Ser Leu Leu Pro
165 170 175
Leu Pro Ser Gly Ile Ser Leu Asn His Ser Lys Lys Ser Ile His Phe
180 185 190
Glu Gly Ile Ala Glu Tyr Phe Tyr Gly Thr Gly Thr Lys Phe His Ile
195 200 205
Thr Val Ser Asp Lys Pro Ser Ser Gln Gly Thr Gln Glu His Lys Ser
210 215 220
Ile Asp Thr Lys Thr Gly Leu Gly Thr Tyr Pro Ile Gly Leu Asn Gly
225 230 235 240
Lys Asn Leu Asn Leu Asp Leu Asn Asp Leu Lys Glu His Met Asp Pro
245 250 255
Lys Asp Phe Glu Lys Leu Lys Glu Leu Gln Asn Glu Lys Val
260 265 270
<210> 192
<211> 373
<212> БЕЛОК
<213> Bacillus sp.
<400> 192
Val Gly Glu Ser Met Asn Phe Lys Val Gly Thr Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Ser Gly Asn Ser Thr Ala
20 25 30
Asn Asn Gly Asn Val Gln Gln Tyr Glu Phe Ile Pro Asp Gly Pro Ser
35 40 45
Glu Arg Phe Phe Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Ile Ser Gly Asn Ser Thr Ala
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ser Pro Ser
85 90 95
Glu Tyr Trp Tyr Phe His Arg Glu Ala Asp Gly Tyr Tyr Val Ile Glu
100 105 110
Ser Lys Leu Ser Gly Lys Val Leu Asp Ile Lys Gly Asn Ser Thr Ala
115 120 125
Asn Asn Ala Asn Val Gln Gln Tyr Gln Phe Ile Pro Asp Ser Pro Ala
130 135 140
Glu Arg Phe Ala Ala Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Asn Thr Lys Pro Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Ala Glu Thr Glu Arg Val Val Thr Ala Phe Ser Ile Val
180 185 190
Pro Ala Ile Ser Val Lys Asp Pro His Tyr Gly Asn Asp Thr Val Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Ser Glu Lys Tyr Ile Phe
225 230 235 240
Glu Thr Lys Thr Gly Ile Lys Gln Ile Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Trp Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly
260 265 270
Phe Ser Leu Gly Leu Ser Thr Gln Tyr Ser Thr Gln Leu Gln Asn Ser
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Val Asn Arg His Glu
290 295 300
Ile Ile Asn Pro Phe Ser Glu Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Ser Val Gln Arg Lys Asn Gly Glu Glu Leu Phe Gly
325 330 335
Tyr Tyr Arg Gln Lys His Pro Lys Leu Phe Asp Lys Gln Lys Leu Tyr
340 345 350
Thr Tyr Glu Glu Leu Lys His Arg Ala Val Asn Tyr Cys Ser Ser His
355 360 365
Leu Val Ile His Leu
370
<210> 193
<211> 322
<212> БЕЛОК
<213> Bacillus sp.
<400> 193
Met Lys Lys Asn Lys Lys Lys Gln Ile Leu Ser Leu Ala Met Leu Val
1 5 10 15
Ser Met Gly Ala Ser Phe Ala Ile Ser Ser Pro Asp Ser Val Ser Ala
20 25 30
Ala Gln Ile Ser Pro Val Gln Asn Thr Ala Gln Gln Gln Ser Val Ser
35 40 45
Ile Ser Asp Trp Arg Glu Pro Phe Gln Lys Thr Tyr Lys Ala Ala Lys
50 55 60
Leu Arg Asp Pro Asn Arg Phe Phe Ser Asn Glu Ala Thr Leu Asp Leu
65 70 75 80
Ser Ser Ile Tyr Gln Ser Ser Val Glu Ala Asp Gly Ser Pro Thr Ile
85 90 95
Thr Asn Ser Lys Ser Leu Phe Val Gly Arg Thr Thr Leu Thr Asn Arg
100 105 110
Thr Asn Arg Asp Gln Thr Leu Thr Thr Asp Ser Phe Ser Lys Thr Phe
115 120 125
Gly Asn Ser Val Ser Asn Ser Thr Thr Asn Gly Phe Asn Leu Gly Val
130 135 140
Ser Thr Ser Ala Ser Phe Gly Ile Pro Leu Ile Gly Glu Thr Ser Ile
145 150 155 160
Glu Ile Ser Thr Glu Tyr Asn Phe Ser Asn Thr Ala Glu Lys Thr Lys
165 170 175
Thr Glu Ser Tyr Thr Tyr Thr Ala Ser Pro Gln Asn Ile Val Val Pro
180 185 190
Ala Asn Ser Ser Val Glu Val Ile Val Asn Leu Asn Thr Ala Lys Ile
195 200 205
Ser Gly Asn Val Lys Leu Leu Ser His Val Asp Gly Gln Val Ser Tyr
210 215 220
Arg Asn Asn Lys Tyr Asn Lys Ile Ser Thr Ser Phe Gly Gly Phe Leu
225 230 235 240
Lys His Ser Val Arg Pro Asn Glu Ile Ala Ser Asn Val Lys Val His
245 250 255
Pro Thr Ser Lys Gly Ala Tyr Leu Ile Gly Lys Gly Lys Tyr Ser Ala
260 265 270
Glu Tyr Gly Thr Glu Phe Ser Val Thr Val Arg Pro Val Ala Lys Pro
275 280 285
Asn Thr Leu Ala Ala Lys Ser Ser Ser Met Val Ser Glu Asn Ser Lys
290 295 300
Ala Thr Ser Glu Gly Tyr Thr Tyr Thr Val Lys Pro Glu Val Lys Lys
305 310 315 320
Glu Gln
<210> 194
<211> 355
<212> БЕЛОК
<213> Bacillus sp.
<400> 194
Met Ala Ile Leu Asn Asn Val Tyr Tyr Val Ile Ala Leu Lys Ala Asn
1 5 10 15
Asp Leu Leu Val Trp Asp Ile Lys Gly Glu Ser Arg Asp Asn Gly Val
20 25 30
Pro Val Leu Leu Tyr Gln Ser Arg Gln Ser Asp Asn Gln Leu Phe Leu
35 40 45
Phe Phe Glu Leu Asp Asn Gly Lys Tyr Ala Ile Val Asn Tyr Asn Ser
50 55 60
Gly Lys Cys Ile Tyr Phe Asp Tyr Arg Ile Tyr Ser Pro Gly Leu Gly
65 70 75 80
Ala Gly Gln Thr Lys Trp Thr Gly Ala Asp Ser Gln Gln Trp Tyr Leu
85 90 95
Arg Ser Val Asp Pro Ser His Asn Gln Tyr Glu Ile Val Asn Gln Gly
100 105 110
Phe Gly Thr Val Ala Ser Tyr Ala Gly Asp Gly Ser Val Lys Tyr Val
115 120 125
Asp Ser Asp Asp Ala Asn Pro Ser Asp Pro Asp Arg Val Phe Gly Ile
130 135 140
Asn Pro Thr Thr Arg Tyr Pro Ile Thr Leu Pro Thr Leu Pro Ala Ile
145 150 155 160
Gly Thr Arg Pro Ala Val Pro Asp Tyr Phe Ser Ser Pro Tyr Asp Thr
165 170 175
Met Pro Ala Thr Ser Ala Ser Gln Ile Val Gly Ala Ser Leu Ile Pro
180 185 190
Cys Ile Met Val Asn Asp Gly Gln Gln Ala Asp Tyr Thr Lys Ile Gln
195 200 205
Asn Ser Pro Tyr Tyr Lys Leu Ile Lys Glu Glu Tyr Trp Glu Gln Thr
210 215 220
Tyr Ser Leu Val Leu Ala Pro Asn Gln Lys Arg Gly Tyr Ser Tyr Thr
225 230 235 240
Thr Gly Thr Ser Ser Val Asp Gln Thr Arg Met Thr Asn Thr Leu Asn
245 250 255
Met Glu Ile Gly Ala Asp Phe Gly Leu Lys Phe Asn Asp Val Thr Ala
260 265 270
Lys Ile Ser Gly Lys Ile Ser Arg Thr Ile Gln Thr Glu Val Ser Thr
275 280 285
Thr Asn Gln Gln Ser Val Ser Glu Thr Lys Ser Asp Glu Ile Asn Asn
290 295 300
Gly Thr Ser Asn Tyr Thr Ile Gly Tyr Thr Gln Tyr Gln Leu Val Thr
305 310 315 320
Lys Tyr Thr Leu Tyr Arg Met Asp Gly Thr Ala Val Ser Asn Pro Phe
325 330 335
Ile Ser Lys Asn Asp Lys Ile Ala Val Thr Arg Ser Arg Ser Gln Leu
340 345 350
Ala Thr Gly
355
<210> 195
<211> 411
<212> БЕЛОК
<213> Bacillus sp.
<400> 195
Leu Ser Thr Lys Leu Gly Gly Thr Ile Met Lys Ser Ile Ser Lys Lys
1 5 10 15
Val Met Ala Gly Leu Leu Val Gly Ala Thr Ser Leu Ser Ile Trp Ala
20 25 30
Pro Ile Ser Glu Ala Ala Ala Pro Glu Asn Asn Arg Tyr Tyr Asn Ile
35 40 45
Ala Leu Lys Ser Asn Pro Lys Lys Val Trp Asn Val Ser Gln Ala Ser
50 55 60
Asn Asp Asn Asp Arg Ala Ile Val Leu Trp Gln Gly Gly Ser Ala Asp
65 70 75 80
His Glu Arg Phe Ala Phe Phe Gln Leu Asp Gly Gly Ala Tyr Ala Ile
85 90 95
Val Asn Lys Asn Ser Gly Lys Val Val Thr Phe Gly Asn Gly Ser Trp
100 105 110
Val Ser Gly Gly Pro Ile Pro Ala Asn Glu Thr Leu Gln Gln Gln Lys
115 120 125
Trp Thr Gly Glu Ala Lys Gln Lys Trp Asn Leu Arg Asp Leu Gly Ser
130 135 140
Asn Gly Gln Gly Ser Asn Asn Tyr Glu Ile Val Asn Gln Ala Asn Gly
145 150 155 160
Lys Val Pro Ser Tyr Arg Trp Ile Gly Ser Val Val Asp Asn Thr Glu
165 170 175
Tyr Leu Gln Leu Asp Asn Ser Asn Pro Ser Asp Pro Asp Lys Val Phe
180 185 190
Thr Leu Phe Asn Gly Thr Leu Asn Ile Phe Gly Gln Asn Met Thr Phe
195 200 205
Phe Asn Asp Thr Ile Ser Val Pro Lys Leu Pro Ala Thr Gly Thr Arg
210 215 220
Pro Asp Ala Pro Asn Tyr Thr Gly Gly Val Asp Gln Gln Leu Pro Gln
225 230 235 240
Val Ser Asp Ser Val Val Val Gly Ala Ser Leu Ile Pro Cys Ile Met
245 250 255
Val Asn Asp Asn Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro
260 265 270
Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Tyr Ser Ala
275 280 285
Val Ile Pro Ala Gly Val Thr His Asn Tyr Ser Phe Thr Thr Gly Met
290 295 300
Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Ser Ile
305 310 315 320
Gly Ala Asp Phe Gly Leu Ser Phe Gly Gly Ala Ser Ala Ser Leu Lys
325 330 335
Thr Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile Thr Ser Thr Asn Thr
340 345 350
Asn Ala Ser Glu Glu Lys Val Ala Arg Ala Val Glu Ser Glu Pro Gly
355 360 365
Lys Thr Thr Gly Phe Thr Glu Tyr Gln Leu Ala Thr Arg Tyr Thr Leu
370 375 380
Lys Arg Ala Asp Gly Ser Asn Val Ser Asp Pro Trp Val Val Lys Asn
385 390 395 400
Asn Lys Ile Thr Ile Thr Arg Lys Asn Ala Gln
405 410
<210> 196
<211> 380
<212> БЕЛОК
<213> Bacillus sp.
<400> 196
Val Ser Asn Glu Asn Ser Ser Lys Glu Asn Leu Asn Thr Pro Leu Ile
1 5 10 15
Asn Asp Glu Asp Leu Ile Pro Val Glu Ile Lys Ala Ser Gly Val Arg
20 25 30
Ile Ser Phe Pro Asp Phe Tyr Phe Tyr Lys Ile Arg Thr Phe Cys Gly
35 40 45
Lys Tyr Ile Asp Ile Gln Asp Ala Ser Thr Ala Asn Asn Lys Ala Ala
50 55 60
Ile Gln Tyr Thr Ala Asn Asn Asn Asn Asn Gln Lys Phe Leu Ile Phe
65 70 75 80
Thr Leu Asp Asn Asn Tyr Ser Val Ile Ala Ala Lys His Ser Gly Lys
85 90 95
Val Leu Asp Ile Met Asp Ile Pro Leu Gly Pro Gly Leu Pro Phe Met
100 105 110
Leu Ile Gln Tyr Asp Phe Gln Asn Gly Asp Asn Gln Lys Phe Phe Ile
115 120 125
Ala Asn Asp Gly Ala Ile Ala Val Lys Lys Thr Gly His Val Trp Asp
130 135 140
Val Lys Ser Gly Ser Thr Lys Asp Asp Thr Pro Ile Ile Pro Tyr Asn
145 150 155 160
Tyr Ser Gly Ala Ser Asn Gln Lys Phe Ser Leu Glu Asn Ser Gly Thr
165 170 175
Ala Ser Val Lys Pro Pro Glu Leu Gly Asp Leu Pro Asn Ser Pro Asp
180 185 190
Phe Lys Thr Asn Asp Leu Asn Glu Ile Leu Pro Asp Asn Thr Thr Pro
195 200 205
Val Ile Thr His Ala Thr Tyr Leu Pro Tyr Phe Met Val Lys Asp Pro
210 215 220
Tyr Tyr Asn Thr Gln Gln Lys Met Gln Asn Ser Pro Tyr Tyr Ile Leu
225 230 235 240
Val Arg Arg Gln Tyr Trp Glu Lys Ile Ser Gln Arg Ile Val Gly Ala
245 250 255
Gly Glu Ser Tyr Lys Tyr Glu Gln Thr Thr Gly Val Ala Arg Thr Asp
260 265 270
Gln Ile Ser Met Thr Glu Thr Thr Glu Ile Ala Ile Gly Ala Asp Leu
275 280 285
Gly Phe Met Phe Lys Gly Phe Ser Ala Asn Leu Ser Thr Ser Ile Thr
290 295 300
Lys Thr Leu Ser Val Thr Lys Ser Thr Ser Thr Thr Glu Ser Val Ser
305 310 315 320
Lys Thr Glu Thr Val Thr Asp Ser Asn Pro Phe Met Tyr Thr Ile Ala
325 330 335
Arg Ala Lys Tyr Met Leu Ile Asn Glu Tyr Tyr Val Thr Arg Ala Asn
340 345 350
Gly Gln Leu Ile Thr Ser Gly Asp Ala Thr Tyr Trp Lys Val Pro Asn
355 360 365
Pro Asn Gln Thr Val Thr Arg Thr Ile Pro Gln Pro
370 375 380
<210> 197
<211> 401
<212> БЕЛОК
<213> Bacillus sp.
<400> 197
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ile Ser Glu Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Asn Ile Ala Leu Lys Ser Asn Thr Lys Lys Val
35 40 45
Trp Asn Val Ser Gln Ala Ser Asn Asp Asn Asp Arg Ala Ile Val Leu
50 55 60
Trp Gln Gly Gly Ser Ala Asp His Glu Arg Phe Ala Phe Phe Gln Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Val Val
85 90 95
Thr Phe Gly Thr Gly Ser Trp Val Ser Gly Gly Pro Ile Pro Ala Ser
100 105 110
Glu Thr Leu Gln Gln Gln Lys Trp Thr Gly Glu Ala Lys Gln Lys Trp
115 120 125
Asn Leu Arg Asp Leu Gly Ser Asn Gly Gln Gly Ser Asn Asn Tyr Glu
130 135 140
Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg Trp Ile Gly
145 150 155 160
Ser Val Val Asp Asn Thr Glu Tyr Leu Gln Leu Asp Asn Ser Asn Pro
165 170 175
Ser Asp Ala Asp Lys Val Phe Thr Leu Phe Asn Gly Thr Met Asn Leu
180 185 190
Gly Gly Met Asp Phe Thr Phe Phe Asn Asp Thr Ile Ser Val Pro Lys
195 200 205
Leu Pro Ala Thr Gly Thr Arg Pro Glu Ala Pro Val Tyr Thr Gly Gly
210 215 220
Ala Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala
225 230 235 240
Ser Leu Ile Pro Cys Phe Met Val Asn Asp Asn Gln Ala Ser Asp Phe
245 250 255
Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr
260 265 270
Trp Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu Ser
275 280 285
Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr
290 295 300
Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Leu Gly Ile Lys Phe Gly
305 310 315 320
Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr
325 330 335
Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr
340 345 350
Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln
355 360 365
Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val Ser
370 375 380
Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys Thr
385 390 395 400
Leu
<210> 198
<211> 486
<212> БЕЛОК
<213> Bacillus sp.
<400> 198
Met Thr His Pro Gln Glu Phe Leu Asn Glu Thr Gln Ser Thr Thr Tyr
1 5 10 15
Gln Ser Ile Gly Ala Gly Glu Val Phe Lys Val Glu Asn Asp Gly Val
20 25 30
Val Lys Val Leu Lys Asp Met Leu Phe Asp Lys Asp Thr Leu Lys Ser
35 40 45
Ile Ala Ser Leu Gly Gly Asp Thr Leu Lys Gln Val Tyr Lys Asp Ala
50 55 60
Tyr His Ser Asn Asp Phe Ser Gly Thr Leu Arg Thr Val Val Leu Gly
65 70 75 80
Ala Val Ala Leu Ile Pro Tyr Gly Gly Ala Phe Ile Ser Gln Ile Leu
85 90 95
Gly Ala Val Cys Pro Asn Asn Val Glu Asp Asn Gln Met Lys Tyr Val
100 105 110
Gln Asp Gln Ile Asn His Leu Asp Lys Lys Ile Glu Asp Leu Ser Ala
115 120 125
Ala Thr Leu Lys Ser His Tyr Glu Thr Leu Leu Lys Glu Leu Glu Ser
130 135 140
Phe Glu Arg Ser Val Asn Ser Leu Asp Thr Thr Asp Val Tyr Tyr Ser
145 150 155 160
Thr Gly Asn Val Tyr Glu Asn Arg Arg Trp His Ala Arg His Ile Asn
165 170 175
Gln Lys Phe Lys Glu Leu Ile Arg Asp Cys Asn Lys Glu Ile Leu Gln
180 185 190
Ala Lys Glu Leu Pro Met Tyr Thr Thr Val Ala Thr Ala Tyr Leu Leu
195 200 205
Phe Leu Lys Phe Ile Glu Lys Asn Gly Lys Gly Pro Lys Ile Lys Phe
210 215 220
Asp Asn Ala Ser Phe Asn Glu Glu Phe Met His Asp Ile Gln Thr Ala
225 230 235 240
Ala Lys Glu Tyr Lys Ile His Ile Glu Tyr Thr Tyr Asn Ala Glu Ala
245 250 255
His Arg Leu Arg Glu Glu Met Ile Ala Ile Ala Gln Lys Ala Arg Ser
260 265 270
Val Thr His Ala Tyr Leu Thr Gly Asn Glu Ser Ser Phe Asp Asp Ala
275 280 285
Val Val Arg Ala Leu Lys Lys Met Glu Asp Lys Tyr Asn Glu Leu Leu
290 295 300
Lys Asn Phe Met Arg Asp Gly Ala Ala Ser Val Leu Ser Gly Gln Asn
305 310 315 320
Thr Ile Met Asn Asn Leu Gly Lys Asp Ile Asp Asn Tyr Arg Asn Lys
325 330 335
Leu Asn Glu Gln Asn Lys Tyr Tyr Asn Arg Thr Trp Gly Asn Gln Ala
340 345 350
Phe Arg Val Ile Ala Arg Ile Asp Thr Trp Val Gln Glu Ser Gly Lys
355 360 365
Trp Tyr Tyr Tyr Asp His Asp Met Leu Leu Val Asn His Ile Phe Tyr
370 375 380
Ser Gly Gly Lys Trp Tyr Tyr Leu Ser Pro Glu Lys Thr Asp Lys Leu
385 390 395 400
Glu Lys Gly Gln Met Ala Thr Gly Trp Leu Ser Leu Pro Ser Asn Lys
405 410 415
Met Gly Val Val Met Met Phe Met Tyr Ser Lys Asn Val Gly Gly Lys
420 425 430
Tyr Ser Asn Glu Leu Pro Lys Ile Met Glu Leu Met Lys Lys Ser Ala
435 440 445
Asn Thr Lys Phe Trp Leu Tyr Phe Ser Pro Asn Gly Glu Leu Val His
450 455 460
Asn Thr Lys Lys Thr Ile Gly Gly Lys Glu Tyr Glu Phe Asp Lys Tyr
465 470 475 480
Gly Ile Cys Leu Asn Pro
485
<210> 199
<211> 403
<212> БЕЛОК
<213> Bacillus sp.
<400> 199
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ile Ser Glu Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Asn Ile Ala Leu Lys Ser Asn Ala Lys Lys Val
35 40 45
Trp Asn Val Ser Gln Ala Ser Asn Asp Asn Asp Arg Ala Ile Ile Leu
50 55 60
Trp Gln Gly Gly Ser Ala Asp His Glu Lys Phe Ala Phe Phe Gln Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Val Val
85 90 95
Thr Phe Gly Thr Gly Ser Trp Val Ser Gly Gly Pro Ile Pro Ala Ser
100 105 110
Glu Thr Leu Gln Gln Gln Ser Trp Thr Gly Glu Ala Lys Gln Lys Trp
115 120 125
Asn Leu Arg Asp Leu Gly Ser Asn Gly Gln Gly Ser Asn Asn Tyr Glu
130 135 140
Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg Trp Met Gly
145 150 155 160
Ser Ile Val Asp Asn Thr Glu Phe Leu Gln Leu Asp Asn Ser Asn Pro
165 170 175
Ser Asp Ala Asp Lys Val Phe Thr Leu Phe Asn Gly Thr Ile Asn Leu
180 185 190
Gly Gly Met Asp Phe Thr Phe Phe Lys Asp Thr Ile Pro Val Pro Lys
195 200 205
Leu Pro Ala Thr Gly Thr Arg Pro Glu Ala Pro Val Tyr Thr Gly Gly
210 215 220
Ala Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala
225 230 235 240
Ser Leu Ile Pro Cys Phe Met Val Lys Asp Asn Gln Ala Asn Asp Tyr
245 250 255
Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr
260 265 270
Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Tyr Thr Arg Asn
275 280 285
Tyr Thr Phe Lys Ser Gly Met Thr Ser Val Asp Gln Gln Lys Met Thr
290 295 300
Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe Glu
305 310 315 320
Gly Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr Ile Gln Thr
325 330 335
Glu Ile Thr Ser Thr Asp Thr Gln Val Ser Glu Glu Thr Val Ala Ser
340 345 350
Thr Leu Thr Ser Pro Ala Gly Lys Thr Thr Gly Phe Thr Glu Tyr Gln
355 360 365
Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Thr Leu Val Ser
370 375 380
Asp Pro Trp Ile Val Lys Asn Asn Lys Ile Thr Ile Val Arg Asp Lys
385 390 395 400
Ala Gln Glu
<210> 200
<211> 409
<212> БЕЛОК
<213> Pseudomonas sp.
<400> 200
Met Glu Ile Asp Leu Glu Thr Ala Ala Lys Glu Leu Glu Val Arg Gly
1 5 10 15
Ala Gln Val Phe Ser Gln Leu Glu Gly Ile Ser Gly Ile Val Asn Ala
20 25 30
Ala Arg Phe Val Pro Val Gln Tyr Phe Glu Lys Lys Val Glu Asn Ile
35 40 45
Asp Asp Glu Thr Phe Ile Val Lys Lys Ser His Val Val Met Ile Lys
50 55 60
Arg Tyr Val Arg Ala Gly Leu Ser Leu Pro Val Asn Leu Gly Glu Ile
65 70 75 80
Glu Asp Tyr Leu Gly Tyr Lys Asn Ala Gly His Pro Leu Leu Glu Pro
85 90 95
Thr Ser Met Gln Gln Phe Tyr Gln Glu Ile His Ser His Ala Leu Ser
100 105 110
Trp Ala Thr Leu Glu Arg Glu Thr Lys Asn Ile Gly Asn Arg Leu Asp
115 120 125
Leu Phe Ala Arg Ser Phe Phe Glu Ser Gly Ser Ala Ile Ile Asn Leu
130 135 140
Leu Lys Glu Thr Glu Thr Tyr Arg Arg Val Glu Gly Asn Ile Asp Ser
145 150 155 160
Leu Asn Asp Asp Glu Lys Thr Gln Leu Ala Ser Ile Pro Leu Ser Ser
165 170 175
Thr Asp Thr Lys Arg Val Glu Ser Leu Arg Lys Ala Leu Lys Tyr Ala
180 185 190
Lys Lys Asp Val Ala Glu Phe Tyr Leu Ala Val Ala Asp Val Asn Ala
195 200 205
Met Ala Val Glu Phe Ser Arg Lys Ile Ser Asp Gly Leu Leu Ile Lys
210 215 220
Leu Glu His Lys Leu Lys Ala Val Asp Glu Val Ser Ile Asp Lys His
225 230 235 240
Glu Gly Ile Asp Arg Leu Arg Glu Gln Ile Lys Asp Leu Asp Glu Ala
245 250 255
Ile Ala Glu Lys Gln Thr Glu Tyr Asp Tyr Gln Val Gly Tyr Ala Phe
260 265 270
Thr Gly Leu Val Phe Gly Pro Leu Gly Leu Ile Val Thr Gly Gly Ile
275 280 285
Phe Gly Ala Glu Ala Glu Lys Thr Arg Ala Gln Lys Asn Glu Leu Leu
290 295 300
Glu Gln Lys Asn Arg Leu Val Thr Gln Met Ser Ser Ala Arg Leu Ser
305 310 315 320
Gln Asn Leu Ile Thr Leu Thr Thr Asn Phe Ser Glu Met Arg Ala Leu
325 330 335
Met Lys Asp Ala Glu Asn Gly Ile Lys Cys Ile Glu Asp Val Leu Gly
340 345 350
Ile Val Trp Asn Ser Leu Gly Val Ser Leu Gly Glu Phe Glu Asp Gly
355 360 365
Ala Ser Asp Leu Asp Leu Ile Leu Leu Val Leu Asn Leu Gln Ala Ile
370 375 380
Phe Asn Pro Trp Lys Thr Ile Gln Gly His Ala His Ala Leu Ser Thr
385 390 395 400
Val Phe Asn Gln Val Val Glu Gly Lys
405
<210> 201
<211> 376
<212> БЕЛОК
<213> Bacillus sp.
<400> 201
Met Lys Lys Gln Lys Ile Lys Lys Tyr Asp Glu Ile Leu Thr Gly Glu
1 5 10 15
Lys Lys Met Lys Arg Tyr Lys Lys Leu Leu Met Val Ala Pro Leu Ala
20 25 30
Cys Met Leu Gly Thr Gly Val Val Thr Leu Pro Thr Val Ser His Ala
35 40 45
Asp Ala Ala Pro Val Ser Ser Val Val Asn Asp Val Phe Arg Gln Gly
50 55 60
Ser Thr Tyr Ser Thr Glu Asn Ile Thr Gln Ala Leu Tyr Val Arg Leu
65 70 75 80
Arg Asp Gly Ile Glu Ala Ser Pro Glu Leu Arg Gln Lys Phe Lys Ile
85 90 95
Ala Asp Asp Glu Lys Ile Gln Phe Glu Ala Asp Ala Gly Asn Phe Asn
100 105 110
Gly Val Lys Thr Pro Ala Ile Asn Tyr Gly Leu Arg Asp Gln Leu Ser
115 120 125
Lys Val Thr Ala Ser Ser Ile Gly Lys Asn Ile Ser Val Asn Pro Thr
130 135 140
Thr Val Thr Glu Val Glu Ala Gly Lys Ile Glu Leu Leu Thr Tyr Arg
145 150 155 160
Asn Glu Thr Pro Leu Thr Gln Lys Gln Tyr Thr Thr Glu Lys Ser Val
165 170 175
Lys Arg Ile Asp Thr Val Thr Ala Thr Asn Gln Tyr Gly Phe Lys Leu
180 185 190
Gly Phe Glu Ser Asn Thr Glu Phe Lys Val Asp Ala Leu Ile Ala Ser
195 200 205
Ala Lys Gln Ser Phe Lys Ala Ser Ala Glu Phe Ser Phe Ser His Thr
210 215 220
Asp Thr Asn Thr Val Thr Asn Glu Glu Thr Val Thr Phe Lys Ala Gln
225 230 235 240
Glu Val Leu Ala Ala Ser Gly Gly Thr Thr Asn Tyr Phe Thr Arg Val
245 250 255
Gly Lys Ala Lys Phe Ser Gly Ser Phe Gln Thr Asp Ala Ala Leu Thr
260 265 270
Asp Leu Lys Leu Lys Leu Pro Ile Thr Lys Lys Asn Gln Asn Gln Thr
275 280 285
Val Ala His Thr Glu Glu Ala Thr Leu Thr Ser Gln Asp Ile Tyr Thr
290 295 300
Ile Phe Lys Asn Thr Pro Tyr Ala Pro Leu Pro Pro Tyr Leu Ser Phe
305 310 315 320
Asp Asp Asn Gly Lys Lys Val Leu Val Lys Asn Val Thr Phe Asp Tyr
325 330 335
Thr Gly Glu Met Gly Tyr Asn Thr Ala Ala Gln Ala Glu Phe Ile Pro
340 345 350
Phe Asp Gln Glu Lys Pro Thr Gln Thr Met Ser Tyr Glu Lys Tyr Glu
355 360 365
Ala Lys Ala Gln Asp Lys Ser Leu
370 375
<210> 202
<211> 348
<212> БЕЛОК
<213> Bacillus sp.
<400> 202
Met Leu Ser Asn Asn Ser Phe Ala Leu Asn Thr Thr Tyr Thr Ile Val
1 5 10 15
Asn Lys Glu Phe Asn Leu Val Phe Asp Ile Glu Ser Leu Ser Asn Gly
20 25 30
Ser Pro Ile Leu Leu Arg Leu Pro Asn Ser Lys Lys Asn Gln Gln Phe
35 40 45
Ile Cys Phe Lys Leu Asp Gly Gly Leu Tyr Thr Ile Ala Asn Gln Gln
50 55 60
Ser Gly Lys Ile Ile Asp Leu Arg Gly Phe Thr Asn Ser Tyr Thr Leu
65 70 75 80
Val Gln Asn Ile Trp Asn Gly Gln Ser Ser Gln Ile Trp Tyr Val Asn
85 90 95
Asn Ser Asn Ile Pro Glu Pro Tyr Tyr Lys Ile Thr Asn Gln Gln Asn
100 105 110
Gly Lys Val Ala Gln Ser Met Asn Asp Ser His Asp Met Asn Pro Tyr
115 120 125
Val Thr Leu Thr Ser Trp Asn Gly Lys Asp Glu Gln Gln Trp His Phe
130 135 140
Ile Pro Lys Asp Ser Ile Glu Phe Pro Asn Ile Pro Glu Lys Gln Pro
145 150 155 160
Leu Pro Asn Pro Pro Glu Tyr Phe Ser Asn Pro Tyr Glu Val Leu Pro
165 170 175
Leu Gln Thr Thr Pro Val Ile Ile Ala Phe Ala Phe Leu Pro Cys Ile
180 185 190
Phe Val Gln Asp Asn Glu Trp Asp His Gln Thr Lys Ile Gln His Ser
195 200 205
Pro Tyr Tyr Thr Phe Val Lys Glu Gln Tyr Trp Glu Arg Ile Asp Ser
210 215 220
Thr Thr Leu Glu Pro Gly Gln Thr Asn Thr Phe Gln Tyr Lys Thr Gly
225 230 235 240
Ile Lys Thr Thr Asp Gln Gln Ser Met Met Asn Thr Ile Gly Met Ser
245 250 255
Ile Gly Ala Asp Leu Gly Phe Gln Phe Asn Gln Thr Ser Ile Lys Ile
260 265 270
His Thr Gln Phe Leu Ser Ala Leu Glu Ala Thr Lys Ser Thr Thr Ser
275 280 285
Glu Gln Leu Ser Glu Thr Thr Ile Thr Asp Ser Ile Thr Asn Thr Lys
290 295 300
Pro Thr Lys Ile Gly Ile Thr Lys Tyr Gln Leu Ile Thr Arg Tyr Ser
305 310 315 320
Leu Leu Arg Thr Asp Asp Ser Leu Val Thr Glu Pro Trp Lys Ile Ser
325 330 335
Asp His Phe Thr Thr Lys Asn Arg Ser Ile Thr Gly
340 345
<210> 203
<211> 155
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 203
Met Leu Ser Asn Asn Ser Phe Ala Leu Asn Thr Thr Tyr Thr Ile Val
1 5 10 15
Asn Lys Glu Phe Asn Leu Val Phe Asp Ile Glu Ser Leu Ser Asn Gly
20 25 30
Ser Pro Ile Leu Leu Arg Leu Pro Asn Ser Lys Lys Asn Gln Gln Phe
35 40 45
Ile Cys Phe Lys Leu Asp Gly Gly Leu Tyr Thr Ile Ala Asn Gln Gln
50 55 60
Ser Gly Lys Ile Ile Asp Leu Arg Gly Phe Thr Asn Ser Tyr Thr Leu
65 70 75 80
Val Gln Asn Ile Trp Asn Gly Gln Ser Ser Gln Ile Trp Tyr Val Asn
85 90 95
Asn Ser Asn Ile Pro Glu Pro Tyr Tyr Lys Ile Thr Asn Gln Gln Asn
100 105 110
Gly Lys Val Ala Gln Ser Met Asn Asp Ser His Asp Met Asn Pro Tyr
115 120 125
Val Thr Leu Thr Ser Trp Asn Gly Lys Asp Glu Gln Gln Trp His Phe
130 135 140
Ile Pro Lys Asp Ser Ile Glu Phe Pro Asn Ile
145 150 155
<210> 204
<211> 376
<212> БЕЛОК
<213> Ensifer sp.
<400> 204
Leu Leu Arg Ala Lys Gly Asn Gly Ala Ala Arg Leu Ala Ala Leu Ser
1 5 10 15
Tyr Thr Arg Phe Gly Cys Asn Ala Ala Val Val Arg Gln Gly Glu Ala
20 25 30
Ala Met Asn Glu Val Leu Gly Asn Ile Gly Gln Asn Pro Gln Val Ser
35 40 45
Lys Arg Ala Leu Arg Ser Arg Arg Ser Pro Val Val Phe Ile Asn Ala
50 55 60
Thr Pro Glu Glu Ala Glu Gln Phe Gln Glu Ile Val Ala Ile Asp Asp
65 70 75 80
Ile Asp Tyr Leu Thr Gln Ala Val Ala Leu Thr Ala Leu Phe Asn Gly
85 90 95
Ala Ile Asp Asn Thr Thr Gly Arg Phe Ile Pro Gly Lys Ala Arg Glu
100 105 110
Leu Ile Ala Asn Tyr Asn Glu Ser Leu Asp Ser Asp Ser Gln Arg Tyr
115 120 125
Lys Ile Gly Ile Phe Asn Thr His Gln Thr Thr Leu Thr Gln Gln Asn
130 135 140
Ser Ala Val Ser Ala Met Ile Asn Gln Ile Leu Glu Thr Leu Lys Asn
145 150 155 160
Val Leu Gly Val Ala Leu Gly Thr Thr Ser Val Ala Gln Met Thr Ala
165 170 175
Ala Ile Thr Asp Ala Phe Thr Asn Leu Asp Glu Gln Ser Gly Asp Ala
180 185 190
Trp Ile Phe Trp Glu Lys Lys Thr Ser Asn Lys Thr Thr Tyr Ser Tyr
195 200 205
Ala Ile Leu Phe Ala Phe Gln Asp Ser Ser Thr Gly Lys Leu Met Phe
210 215 220
Ala Leu Pro Met Ser Leu Glu Ile Glu Val Asp Val Arg Tyr Glu Arg
225 230 235 240
Val Leu Phe Ile Thr Val Glu Asp Lys Glu Thr Tyr Ser Val Lys Leu
245 250 255
Asp Thr Met Lys Val Gly Gln Leu Leu Phe Pro Lys Ser Pro Gly Ala
260 265 270
Asn Ala Leu Gln Thr Ala Arg Arg Leu Gly Val Arg Ser Ala Lys Ala
275 280 285
Asp Leu Leu Ala Ala Pro Ala Pro Ile Ser Asn Ile Val Val Thr Asn
290 295 300
Trp Ala Lys Thr Thr Thr Tyr Ala Thr Ala Ala Thr Gly Val Tyr Thr
305 310 315 320
Glu Ser His Pro Leu Val Gln Val Met Ala Val His Pro Asn Val Ser
325 330 335
Asn Pro Leu Gln Asp Gly Asn Asn Tyr Leu Val Ser Phe Thr Ile Asn
340 345 350
Gly Val Gln Gln Thr Arg Gly Leu Leu Leu Asn Gly Ile Met Pro Asp
355 360 365
Gly Glu Leu Trp Phe Val Ser Ile
370 375
<210> 205
<211> 381
<212> БЕЛОК
<213> Bacillus sp.
<400> 205
Met Leu Lys Lys Ile Pro Tyr Arg Ala Met Ala Leu Ser Thr Ile Leu
1 5 10 15
Ala Ile Ser Ala Thr Thr Ile Ala Ser Pro Ala Thr Thr Phe Ala Ile
20 25 30
Gly Ile Glu Gln Ser Asn Thr Glu Asn Lys Glu Leu Ser Gly Asn Thr
35 40 45
Asp Lys Met Lys Gln Thr Leu Val Lys Ala Gly Ala Phe Ala Gln Ala
50 55 60
Met Asn Leu Tyr Ser Tyr Met Leu Leu Lys Asn Pro Asp Val Asn Phe
65 70 75 80
Ala Gly Leu Asp Pro Asn Thr Asp Leu Ala Gly His Gly Asp Leu Pro
85 90 95
Gly Lys Leu Leu Glu Ser Gln Arg Gln Ala Arg Ala His Ala Ser Thr
100 105 110
Trp Asp Lys Thr Val Lys Arg Gln Leu Leu Asp Thr Leu Thr Gly Ile
115 120 125
Ile Thr Phe Asp Thr Thr Phe Gln Ala Tyr Tyr Gln Thr Leu Val Asp
130 135 140
Ser Val Asp Lys Gly Asp Lys Glu Thr Leu Lys Glu Gly Ile Lys Asp
145 150 155 160
Leu Gln Arg Asp Val Gln Lys Asn Lys Lys Cys Ala Val Ser Leu Leu
165 170 175
Asp Ala Leu Lys Asp Leu Lys Thr Ala Ile Asp Glu Asp Asn Arg Ser
180 185 190
Phe Gln Glu His Gln Thr Thr Leu Ser Ser Ile Leu Lys Gly Lys Thr
195 200 205
Ala Asp Thr Gly Ile Glu Ala Asp Glu Ala Asn Leu Lys Lys Ile Met
210 215 220
Glu Asp Ile Asn Tyr Tyr Arg Lys Tyr Gln Met Asp Leu Ser Pro Leu
225 230 235 240
Ser Ile Leu Gln Trp Ile Ile Ser Pro Ile Gly Ala Gly Val Ser Val
245 250 255
Ala Val Ala Lys Thr His Leu Asp Lys Leu Arg Pro Ala Phe Glu Glu
260 265 270
Leu Asn Lys Met Ile Asp Gln Lys Arg Thr Val Asn Arg Ile Val Phe
275 280 285
Thr Ala Ala Gln Asn Val Asn Glu Leu Ala Asn Ala Ile Asp Gly Ala
290 295 300
Thr Gln Ser Leu Gln Tyr Met Val Asp Gln Trp Asn Asp Leu Asp Ser
305 310 315 320
Gln Tyr Asp Gly Val Leu Asp Ser Ile Glu Thr Ala Asp Ala Lys Ile
325 330 335
Gln Glu Asp Arg Phe Lys Phe Leu Lys Pro Thr Leu Lys Thr Ala Lys
340 345 350
Asp Asn Trp Asn Ala Leu Lys Thr Asp Ala Asp Thr Leu Lys Glu Gly
355 360 365
Leu Lys Glu Leu Lys Ile Glu Pro Ala Asn Thr Gln Asn
370 375 380
<210> 206
<211> 377
<212> БЕЛОК
<213> Ensifer sp.
<400> 206
Leu Leu Arg Thr Lys Ala Asn Gly Ser Ala Cys Arg Val Glu Leu Ser
1 5 10 15
Tyr Thr Arg Phe Gly Cys Asn Cys Ala Ala Met Lys Gln Gly Glu Ala
20 25 30
Ala Met Asn Glu Val Leu Ala Asn Asn Ile Gly Gln His Pro Gln Val
35 40 45
Ser Arg Arg Ala Leu Arg Ser Pro Arg Ser Pro Val Val Phe Ile Asn
50 55 60
Thr Thr Ser Glu Glu Ala Glu Gln Phe Gln Glu Ile Val Ala Ile Asp
65 70 75 80
Asp Ile Asp Tyr Leu Thr Gln Ala Val Ala Leu Thr Ala Leu Phe Asn
85 90 95
Gly Ala Ile Asp Asn Thr Thr Gly Arg Phe Ile Pro Gly Arg Ala Arg
100 105 110
Glu Leu Ile Ala Asn Tyr Asn Glu Ser Leu Asp Pro Glu Ser Gln Arg
115 120 125
Tyr Lys Ile Gly Ile Phe Asn Thr His Gln Thr Thr Leu Thr Gln Gln
130 135 140
Asn Ser Ala Val Ser Ala Met Ile Asn Gln Ile Leu Glu Thr Leu Lys
145 150 155 160
Asn Val Met Gly Val Ala Leu Gly Thr Thr Ser Val Thr Gln Met Thr
165 170 175
Ala Ala Ile Thr Asp Ala Phe Thr Asn Leu Asn Glu Gln Ser Gly Asp
180 185 190
Ala Trp Ile Phe Trp Glu Lys Lys Thr Ser Asn Lys Thr Thr Tyr Ser
195 200 205
Tyr Ala Ile Leu Phe Ala Phe Gln Asp Ser Ser Thr Gly Lys Leu Met
210 215 220
Phe Ala Leu Pro Met Ser Leu Glu Ile Glu Val Asp Val Ser Tyr Glu
225 230 235 240
Arg Val Leu Phe Ile Thr Val Glu Asp Lys Glu Thr Tyr Ser Val Lys
245 250 255
Leu Asp Thr Met Lys Val Gly Gln Leu Leu Phe Pro Lys Ser Pro Gly
260 265 270
Ala Asn Ala Leu Gln Thr Val Arg Arg Leu Arg Thr Arg Ser Gly Ser
275 280 285
Ser Gly Leu Leu Thr Ala Pro Ser Pro Val Thr Asn Ile Val Val Thr
290 295 300
Asn Trp Ala Lys Thr Thr Thr Phe Ala Thr Ser Ala Asn Gly Phe Tyr
305 310 315 320
Thr Glu Asn His Pro Leu Val Gln Leu Met Ala Val Gly Pro Thr Val
325 330 335
Val Asn Pro Leu Tyr Asp Gly Asn Gln Tyr Leu Val Ser Phe Asp Leu
340 345 350
Asn Gly Gly Arg Gln Thr Arg Gly Leu Ile Leu Asn Gly Thr Leu Pro
355 360 365
Asp Gly Glu Leu Trp Phe Val Ser Met
370 375
<210> 207
<211> 383
<212> БЕЛОК
<213> Bacillus sp.
<400> 207
Met Met Gln Phe Asn Lys Arg Asn Glu Asn Asn Ala Glu Phe Tyr Lys
1 5 10 15
Gly Glu Lys Val Thr Pro Phe Glu Tyr Asp Leu Pro Glu Glu Gly Lys
20 25 30
Ala Phe Gln Thr Ile Gly Ser Ile Leu Ser Thr Asn Asn Arg Ile Leu
35 40 45
Val Ala Ala Arg Thr Arg Ser Gly Gln Glu Leu Gly Gln Asp Val Gly
50 55 60
Asp Phe Cys Arg Arg Val Thr Leu Asn Asn Gln Val Val Asn Asp Thr
65 70 75 80
Ala Ala Ala Thr Asn Tyr Glu Ala Gln Phe Ile Phe Phe Lys Lys Asp
85 90 95
Phe Ser Gly Tyr Ile Ile Ala Asn Arg Gln His Gly Glu Val Leu Glu
100 105 110
Ser Val Phe Ala Asn Asn Asp Ser Leu Ile Thr Ala Pro Tyr Ala Asn
115 120 125
Lys Thr Gln Gln Val Phe Gly Lys Ser Leu Tyr Asp Asp Lys Ser Phe
130 135 140
Arg Leu Tyr Gly Asp His Gly Ala Ile Thr Thr Cys Gly Asp Lys Trp
145 150 155 160
Asn Ser Trp Thr Arg Ile Thr Met Ser Gly Ser Asn Glu Asp Gln Ala
165 170 175
Arg Tyr Val Phe Lys His Gly Lys Thr Ser Phe Pro Ile Thr Ile Pro
180 185 190
Thr Lys Leu Glu Pro Thr Thr Leu Gly Pro Ile Pro Glu Leu Thr Asn
195 200 205
Leu Asp Asp Val Gly Thr Pro Pro Ala Pro Ala Val Val Gly Thr Ala
210 215 220
Leu Leu Pro Cys Ile Phe Val Asn Asp Arg Thr Pro Ser Gln Arg Ile
225 230 235 240
Gln Asp Ser Pro Tyr Tyr Val Leu Glu Cys Tyr Asp Tyr Trp Lys Leu
245 250 255
Ile Lys Ser Ser Ile Ile Gln Pro Gly Gly Phe Asp Thr Trp Trp Glu
260 265 270
Glu Thr Gly Ile His Ser Asn Val Gln Ala Ala Met Lys Asn Met Ile
275 280 285
Asn Ile Ser Ile Gly Asp Asp Lys Arg Leu Arg Phe Phe Asn Leu Ser
290 295 300
Asp Pro Phe His Gln Ser Ile Val Glu Asp Ile Asn Ile Pro Ala Ser
305 310 315 320
Thr Ser Asn Ser Asp Leu Ser Phe Ile Thr Ala Ile Arg Glu His Thr
325 330 335
Asn Pro Tyr Ser Thr Arg Leu Arg Phe Ala Lys Tyr Phe Val Ala Arg
340 345 350
Lys Phe Glu Leu Lys Arg Leu Gly Gly Ser Thr Pro Ile Tyr Thr Trp
355 360 365
Glu Tyr Ile Asp Asp Thr Lys Tyr Val Met Lys Glu Phe Arg Ser
370 375 380
<210> 208
<211> 287
<212> БЕЛОК
<213> Bacillus sp.
<400> 208
Met Asn His Leu Ala Phe Ser Pro Arg Glu Leu Gly Gly Lys Asp Ser
1 5 10 15
Pro Phe Leu Leu Thr Ile Glu Glu Trp Arg Ser Phe Ala Asn Tyr Leu
20 25 30
Asn Lys Trp Ile Ser Ala Pro Thr Ser Ile Asp Gln Val Lys Glu Arg
35 40 45
Ile Tyr Leu Ile Leu Asp Gly Lys Val Lys Ser Asn Trp Asp Arg Leu
50 55 60
Ile Ile Thr Ser Thr Phe Arg Arg Leu Val Glu Glu Ala Leu Ala Thr
65 70 75 80
Lys Leu Asn Val Arg Glu Lys Cys Arg Phe Trp Asn Asn Gly Gly His
85 90 95
Lys Asp Ile Leu Ile Leu Ser Gln Asn Ile Val Ala Phe Ala Asp Leu
100 105 110
Ile Thr Ile Lys Tyr Phe Asn Asp Leu Asn Gln Val Ile Leu Glu Ala
115 120 125
Gln Asn Gly Lys Leu Ser Pro Asn Thr Lys Ser Lys Phe Val Asn Met
130 135 140
Cys Lys Asp Leu Ser Asn His Ala Gln Thr Phe Tyr Gln Gln Ser Gln
145 150 155 160
Ser Ile Ala Thr Ser Leu Ser Glu Phe Tyr Ser Glu Ile Gln Lys Tyr
165 170 175
Glu Glu Ile Val Pro Thr Trp Ser Tyr Leu Met Ser Gln Leu Pro Leu
180 185 190
Tyr Asn Ser Asn Asp Phe Val Val Ala Gln Asn Thr Val Val Tyr Leu
195 200 205
Val Ala Pro Val Lys His Leu Ile Gln Phe Lys Glu Asn Val Leu Pro
210 215 220
Val Ile Arg Lys Val His Arg Ile Trp Gly Ser Ile Ser Tyr Asp Leu
225 230 235 240
Lys Glu Leu Ala Asp Asn Ile Glu Asp Ile Glu Asn Leu Glu Glu Phe
245 250 255
Ile Ala Ala Leu Glu Leu Glu Leu Ala Phe Glu Asp Trp Lys Ala Ile
260 265 270
Arg Asn Glu Ala Glu Asn Phe Tyr Lys Asn Ala Glu Asn Phe Ser
275 280 285
<210> 209
<211> 644
<212> БЕЛОК
<213> Bacillus sp.
<400> 209
Leu Leu Tyr Thr Gln Pro Ile Leu Gln Pro Ile Leu Lys Asn Val Thr
1 5 10 15
Ser Ile Leu Asp Val Arg Asn Met Leu Ile Lys Ser Tyr Asn Gln Arg
20 25 30
Gly Arg Tyr Ile Ile Met Lys Tyr Lys Asp Arg Lys Asn Ala Lys Arg
35 40 45
Lys Tyr Lys Gln Ala Leu Leu Ala Thr Val Ala Thr Met Thr Leu Gly
50 55 60
Val Ser Thr Leu Gly Ser Thr Ala Ser Val Phe Ala Glu Glu Asn Thr
65 70 75 80
Gln Gln Gln Gln Ser Ala Met Ala Pro Ala Thr Asp Gly Ser Val Ile
85 90 95
Asn Ile Asp Gly Ser Gly Asp Ile Lys Thr Asp Gly Ile Val Phe Lys
100 105 110
Lys Glu Ser Thr Asp Gly Leu Val Phe Lys Arg Ala Asp Asp Gly Thr
115 120 125
Ile Thr Val Ser Lys Glu Lys Glu Lys Leu Phe Asn Ile Asn Thr Leu
130 135 140
Lys Ala Ile Gly Ser Leu Gly Gly Ala Thr Phe Lys Gln Ala Trp Ala
145 150 155 160
Asp Arg Lys Ala Gly Asn Phe Asn Asn Thr Phe Arg Thr Leu Ala Met
165 170 175
Gly Ser Ala Ala Leu Ile Pro Tyr Gly Gly Val Val Ile Ser Pro Leu
180 185 190
Ile Gly Tyr Leu Trp Pro Glu Asn Asp His Glu Ala Gln Ala Lys Leu
195 200 205
Lys Gln Leu Met Ser Asp Ile Ala Ala Gln Thr Lys Thr Gln Ile Ala
210 215 220
Asp Tyr Asp Val Glu Ala Leu Gly Ser Lys Leu Lys Lys Leu Arg Lys
225 230 235 240
Asp Leu Gln Glu Phe Glu Gly Ile Met Asn Pro Thr Gly Arg Lys Ile
245 250 255
Ala Ala Ala Pro Ser Val Gly Asp Glu Ala Glu Ser Thr Arg Ile Leu
260 265 270
Val Gly Asn Ile His Asn Asp Phe Lys Asn Leu Ile Glu Glu Ser Gln
275 280 285
Lys Glu Ser Tyr Lys Glu Thr Glu Leu Pro Ile Phe Met Val Ala Ala
290 295 300
Thr Ala His Leu Gln Phe Leu Asn Tyr Leu Ala Leu His Gly Lys Asp
305 310 315 320
Pro Lys Leu Asn Tyr Asp Asp Lys Gly Leu Lys Ile Ala Ala Leu Thr
325 330 335
Pro Leu Glu Arg Ala His Lys Glu Tyr Lys Glu Tyr Ala Thr Glu Ala
340 345 350
Ala Lys Lys Gly Leu Asp Ala Ile Asn Asn Ala Glu Arg Pro Val Lys
355 360 365
Gln Met Gln Glu Ser Trp Thr Phe Lys Tyr Leu Ala Ser Asp Leu Lys
370 375 380
Lys Gln Ala Leu Ser Ala Thr Ile Met Gly Pro Asp Thr Val Val Lys
385 390 395 400
Glu Thr Phe Asp Thr Thr Ile Asn Asn Ile Ala Phe Lys Gln Ala Leu
405 410 415
Gly Ile Lys Asp Asp Trp Thr Met Asp Gln Asp Gly Arg Ile Leu Tyr
420 425 430
Tyr Asp Pro Gln Asp His Lys Met Gln Thr Gly Trp Lys Glu Ile Ser
435 440 445
Thr Ser Phe Ala Ala Arg Ile Gln Asn Thr Gly Asn Leu Ser Ile Gln
450 455 460
Phe Leu Lys Asp Gln Gly Leu Lys Tyr Ser Trp Tyr Tyr Phe Ser Pro
465 470 475 480
Glu Lys Thr Asp Lys Phe Ala Lys Gly Glu Leu Tyr Lys Asp Thr Thr
485 490 495
Glu Thr Ile Asn Gly Lys Thr Tyr Thr Phe Gly Val Asp Gly Thr Cys
500 505 510
Leu Asn Pro Asp Gly Gly Glu Ala Ala Gly Trp His Gln Ile Gly Glu
515 520 525
Ala Trp Tyr Phe Tyr Ser Asp Gly Lys Thr Lys Asn Ser Glu Gly Lys
530 535 540
Thr Phe Ala Lys Asn Glu Lys Val Thr Gly Trp Tyr Lys Asp Glu Lys
545 550 555 560
Ala Gly Arg Tyr Tyr Tyr Leu Ser Pro Gly Glu Thr Lys Asn Ser Asp
565 570 575
Lys Lys Val Phe Lys Ala Gly Glu Met Val Thr Gly Trp Tyr Gln Glu
580 585 590
Gly Asn Lys Trp Tyr Tyr Leu Thr Pro Asn Pro Arg Lys Asn Lys Glu
595 600 605
Gly Ser Glu Phe Ala Ala Gly Gln Leu Ile Phe Gly Thr Leu Ser Ile
610 615 620
Glu Asp Lys Asn Gly Glu Tyr Val Gln Tyr Lys Phe Asn Asn Asp Gly
625 630 635 640
Ser Leu Lys Glu
<210> 210
<211> 406
<212> БЕЛОК
<213> Bacillus sp.
<400> 210
Met Ala Asp Asn Leu Asn Glu Asp Gln Asn Glu Asn Thr Val Thr Asn
1 5 10 15
Glu Asp Ser Leu Ala Asn Asp Asp Arg Asn Glu Ser Leu Pro Glu Glu
20 25 30
Ser Arg Met Phe Ala Tyr Tyr Gln Pro Val Asn Thr Asn Asn Lys Val
35 40 45
Trp Asp Ala Ser Glu Gly Gly Gly Gly Asp Phe Ser Gly Gly Cys Gly
50 55 60
Arg Thr Phe Phe Pro Asn Ala Met Tyr Asn Tyr Pro Tyr His Gly Gly
65 70 75 80
Leu Asn Gln Gln Phe Ile Trp Phe Gln Leu Asp Asp Gly Arg Phe Ala
85 90 95
Ile Ala Asn Lys Gln Asn Gly Val Val Leu Gln Lys Ile Pro Phe Gly
100 105 110
Asn Val Lys Glu Ile Ile Gly Phe Pro Trp Gly Gly His Tyr Thr Gln
115 120 125
Met Trp Arg Lys Glu Ser Thr Ser Ala Thr Asn Phe Arg Leu Val Asn
130 135 140
Glu Ala Glu Ala Leu Glu Val Cys Gly Gly Asn Thr Ser Ala Gln Ala
145 150 155 160
Lys Met Ser Phe Ile Pro Asn Arg Pro Asp Asn Ala Leu Leu Phe Arg
165 170 175
Arg Arg Ala Ile Ala Gln Asn Asn Val Val Pro Ala Leu Pro Ser Lys
180 185 190
Gln Pro Leu Pro Ala Leu Pro Ile Leu Ser Asn Leu Asn Asp Pro Ala
195 200 205
Pro Asn Glu Thr Ala Arg Ala Ile Thr Gly Ser Ser Leu Val Pro Cys
210 215 220
Ile Met Val Ser Asp Tyr Ile Pro Val Pro Gln Arg Ile Lys Asp Ser
225 230 235 240
Pro Tyr Tyr Ile Leu Val Lys Lys Gln Phe Trp Lys Arg Leu Trp His
245 250 255
Asp Thr Phe Ile Pro Gly Glu Tyr Arg Glu Phe Gln Glu Thr Thr Gly
260 265 270
Met Ser Lys Thr Gln Gln Glu Ser Met Thr Gln Thr Thr Asn Ile Ser
275 280 285
Leu Gln Ala Asp Phe Gly Phe Ala Phe Lys Ala Leu Thr Leu Gly Leu
290 295 300
Gly Val Ser Ile Ser Arg Ser Ile Glu Ile Ser Arg Ser Thr Thr Asp
305 310 315 320
Thr Val Met Ser Gln Tyr Thr Asp Thr Gln Gly Tyr Lys Asn Gln Asn
325 330 335
Ser Ile Lys Ile Ser Trp Ala Lys Tyr Ala Leu Ala Thr Glu Phe Ile
340 345 350
Leu Met Arg Lys Asp Gly Thr Thr Ala Gly Ser Trp Glu Ala Val Asp
355 360 365
Lys Lys Val Thr Arg Ser Ile Ser Tyr Pro Pro Thr Gln Thr Leu Ser
370 375 380
Trp Glu Lys Ser Leu Glu Asp Gln Lys Asp Asn Thr Val Phe Glu Ser
385 390 395 400
Thr Thr Ile Thr Ser Glu
405
<210> 211
<211> 373
<212> БЕЛОК
<213> Bacillus sp.
<400> 211
Val Gly Lys Ser Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Ser Gly Asn Gln Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Ala Asp Ala Pro Ser
35 40 45
Glu Arg Phe Phe Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Ile Ser Gly Asn Gln Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ala Pro Ser
85 90 95
Glu Tyr Trp Tyr Phe His Arg Glu Ala Asp Gly Tyr Tyr Val Ile Glu
100 105 110
Ser Lys His Ser Gly Lys Val Leu Asp Ile Ser Gly Asn Glu Thr Gly
115 120 125
Asn Asn Ala Asn Val Gln Gln Phe Glu Phe Leu Ala Asp Ala Pro Ser
130 135 140
Glu Arg Phe Ala Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Asn Thr Gln Pro Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Glu Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val
180 185 190
Pro Ala Ile Ser Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Ser Glu Arg Tyr Asp Phe
225 230 235 240
Val Thr Thr Thr Gly Ile Lys Val Thr Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Trp Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly
260 265 270
Phe Ser Leu Gly Met Ser Ser Gln Tyr Ser Gln Glu Leu Gln Thr Ser
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Thr Gln Glu His His
290 295 300
Ile Thr Asn Pro Phe Val Glu Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Tyr Val Gln Arg Lys Asn Gly Thr Ile Val Asn Ala
325 330 335
Pro Trp Thr Met Thr Asp Lys Thr Asn Ala His Ala Val Thr Phe Pro
340 345 350
Lys Ser Thr Gly Lys Val Leu Asn Glu Asn Ile Lys Gln Ile Ser Lys
355 360 365
Ser Glu Ser Leu Asn
370
<210> 212
<211> 1049
<212> БЕЛОК
<213> Bacillus sp.
<400> 212
Met Thr Asn Glu Leu Asp Ser Asn Thr Gln Cys Phe Asn Glu Ile Asp
1 5 10 15
Asn Gly Ile Gln Ala Leu Asp Pro Tyr Thr Phe Tyr Arg Ile Arg Thr
20 25 30
Phe Cys Gly Lys Met Leu Asp Val Val Gly Gln Ser Thr Ala Asp Asn
35 40 45
Ala Met Val Val Gln Tyr Ser Val Asn Asn Lys Lys Asn Gln Asn Phe
50 55 60
Leu Ile Phe Thr Leu Asp Asp Gly Tyr Ser Val Ile Ala Ser Glu Asn
65 70 75 80
Ser Gly Lys Val Leu Asp Ile Ser Gln Ser Leu Ile Phe Lys Gly Ile
85 90 95
Leu Ile Gln Tyr Gly Phe Glu Asn Ala Asp Asn Gln Lys Phe Leu Ile
100 105 110
Ser Asn Asn Gly Thr Ile Ala Val Lys Arg Ser Gly Lys Val Phe Asp
115 120 125
Val Ala Gly Ala Ser Thr Ser Asn Asp Ala Leu Ile Val Glu Tyr Asn
130 135 140
Phe Asn Asn Ala Val Asn Gln Lys Phe Thr Phe Asp Gln Val Lys Asn
145 150 155 160
Phe Gln Val Pro Asn Pro Ser Lys Glu Thr Leu Pro Pro Ala Pro Asp
165 170 175
Phe Lys Asn Asp Ile Asn Glu Gln Leu Pro Asp Lys Thr Asp Pro Val
180 185 190
Ile Thr His Tyr Thr Asn Ile Pro Tyr Phe Met Val Ala Asp Asn Thr
195 200 205
Leu Asn Ala Gln Gln Gln Ile Gln Ile Ser Pro Tyr Tyr Lys Leu Val
210 215 220
Lys Ile Gln Tyr Trp Glu Lys Val Thr Gln Arg Ile Leu Gly Pro Arg
225 230 235 240
Asp Glu Tyr Glu Tyr Gln Lys Arg Lys Gly Ile Ser Arg Thr Asp Gln
245 250 255
Thr Ser Met Thr Glu Thr Val Ser Met Ser Ile Gly Ala Asp Phe Gly
260 265 270
Phe Met Phe Lys Gly Phe Ser Ala Asn Leu Ser Thr Gln Ile Thr Lys
275 280 285
Glu Leu Ser Val Thr Lys Ser Thr Ser Thr Thr Glu Met Thr Glu Glu
290 295 300
Thr Tyr Lys Glu Asn Tyr Thr Asn Pro Phe Asn Tyr Glu Leu Ala Arg
305 310 315 320
Ala Gln Tyr Val Leu Val Asn Glu Phe Tyr Val Thr His Met Asp Gly
325 330 335
Thr Arg Ile Thr Ala Asn Trp Thr Val Arg Asp Asp Thr Arg Thr Val
340 345 350
Thr Arg Val Phe Pro Lys Ser Thr Phe Asn Gly Asp Val Glu Ser Phe
355 360 365
Glu Asp Ser Val Ile Thr Asn Asn Asp Asn Gly Tyr Ala Tyr Asp Ser
370 375 380
Asn Ile Gly Tyr Ser Asn Pro Asn Trp Met Ser Lys Ile Ser Ser Ser
385 390 395 400
Lys Lys Ile Ser Glu Leu Ser Ile Pro Gly Thr His Gly Ser Ile Ala
405 410 415
Leu His Gly Val Thr Phe Ala Asp Glu Asp Trp Val Arg Asn Gln Arg
420 425 430
Met Ser Ile Thr Thr Gln Leu Thr Ser Gly Ile Arg Tyr Leu Asp Ile
435 440 445
Arg Ala Arg Arg Thr Gly Asn Ser Phe Ala Met His His Gly Ser Val
450 455 460
Tyr Gln Lys Lys Met Phe Gly Asp Ile Leu Asn Glu Val Thr Thr Phe
465 470 475 480
Leu His Gln Asn Pro Gln Glu Thr Val Leu Met Arg Leu Lys Glu Glu
485 490 495
His Thr Pro Glu Ala Gly Ser Leu Ser Phe Glu Glu Ile Leu Asn Arg
500 505 510
Tyr Trp Asp Asn Pro Ala Tyr Lys Gln Tyr Phe Trp Lys Pro Pro Ser
515 520 525
Ser Asn Asn Asn Pro Thr Leu Asn Glu Val Arg Gly Lys Ile Val Leu
530 535 540
Ile Gln Asn Phe Asn Ala Ser Lys Lys Phe Gly Ile Ser Tyr Asn Thr
545 550 555 560
Leu Asn Ile Gln Asp Gln Asp Asn Val Asp Asp Thr Pro Asp Ser Met
565 570 575
Tyr Ser Lys Trp Thr Ala Val Gly Asn His Leu Lys Ala Ala Asp Ala
580 585 590
Ser Asn Arg Gln Ser Ile Tyr Leu Asn His Leu Ser Gly Asn Gly Gly
595 600 605
Phe Ser Gly Ala Lys Pro Trp Phe Val Ser Ser Gly Tyr Arg Gly Arg
610 615 620
Gly Thr Asn Asp Gly Lys Gln Met Ile Thr Glu His Arg Asp Pro Asn
625 630 635 640
Lys Trp Pro Gln Phe Pro Arg Gly Tyr Tyr Gly Gln Ile Phe Tyr Gly
645 650 655
Gly Met Asn Ile Met Ala Thr Gln Tyr Ile Gln Gln Trp Pro Ile His
660 665 670
His Ala Gly Ile Ile Ala Ala Asp Phe Pro Gly Lys Gly Leu Ile Asp
675 680 685
Arg Val Ile Arg Leu Asn Glu Arg Phe Met Gly Glu Thr Ile Arg Tyr
690 695 700
Ile Glu Pro Gln Gly Ser Lys Thr Ile Arg Ile Gly Phe Gly Gly Asn
705 710 715 720
Asn Tyr Leu Gln Asn His Tyr Lys Ile Tyr Val Asn Gly Arg Tyr Tyr
725 730 735
Gly Glu Ile Asn Lys Gly His Pro Tyr Tyr Gly Thr Trp His Gln Thr
740 745 750
Asp Phe Gly His Glu Phe Lys Thr Gly Asp Ala Pro Leu Phe Thr Gly
755 760 765
Asp Lys Val Glu Val Phe Ile Lys Asn Ser Asp Gln Gln Thr Leu Ile
770 775 780
Lys Ser Gln Ile Leu Asn Val Asp Glu Gln Glu Gly Glu Glu Ile Arg
785 790 795 800
Val Leu Asp Gly His Phe Ser Ile Arg Ser Ala Leu Asn Thr Ala Lys
805 810 815
Ala Val His Met His Arg Asn Asp Ser Arg Asn Ile Thr Leu Tyr Gln
820 825 830
Tyr Asn Asn Glu Leu His Leu Glu Phe Thr Leu Gln Tyr Asp Glu Ser
835 840 845
Lys Lys Ala Tyr Thr Ile Trp Asn Arg Trp Asp Pro Thr Lys Val Met
850 855 860
Ala Trp Asn Asp Ile Pro Asn Ser Leu Asn Val Phe Gly Thr Pro Phe
865 870 875 880
Asp Pro Asn Lys Asp Glu His Tyr Trp Lys Phe Lys Arg Ala Gly Asn
885 890 895
Ser Tyr Val Tyr Leu Glu Asn Leu Lys Asn Gly Glu Val Leu Asp Val
900 905 910
Thr Gly Gly Ser Ser Thr Asn Asp Thr Asn Ile Gly Val Trp Pro Leu
915 920 925
Asn Gln Lys Asn Asn Gln Lys Phe Lys Leu Val Gln Arg Asn Glu Thr
930 935 940
Lys Lys Ala Ser Ile Val Ser Glu Lys Gly Pro Gln Ser Gly Gln Lys
945 950 955 960
Asn Arg Ser Ser Asn Asn Phe Ser Leu Gly Ser Ala Lys Gly Lys Gln
965 970 975
Val Arg Val Leu Ile Lys Asn Ala Glu Gly Gln Glu Ala Ser Ile Ser
980 985 990
Phe Arg Val Met Arg Asp Lys Ser Ala Asp Thr Asp Pro Val Ile Trp
995 1000 1005
Ser Gly Val Arg Asn Gly Ser Leu Leu Thr Val Pro Thr Asp Ala
1010 1015 1020
Asn Ser Ser Asn Leu Tyr Ile Ala Asn Pro Ser Gly Ala Thr Ser
1025 1030 1035
Asn Phe Thr Val Glu Phe Tyr Thr Leu Glu Asn
1040 1045
<210> 213
<211> 371
<212> БЕЛОК
<213> Bacillus sp.
<400> 213
Met Met Ser Asn Glu Asn Asn Asn Arg Arg Asn Thr Asn Ala Ser Met
1 5 10 15
Gly Ser Asp Gly Glu Ser Ile Gln Asn Lys Ile Gly Asp Ser Arg Ser
20 25 30
Gln Ile Ser Phe Pro Asp Phe Asn Phe Tyr Lys Ile Arg Thr Phe Gly
35 40 45
Gly Lys Tyr Ala Asp Ile Thr Gly Asp Ser Leu Leu Ile Gln Tyr Asp
50 55 60
Gly His Asn Gly Asp Asn Gln Lys Phe Leu Ile Val Thr Leu Asp Asn
65 70 75 80
Asp Tyr Ser Ile Ile Ala Ala Lys His Thr Gly Lys Val Met His Glu
85 90 95
Leu Tyr Leu Leu Tyr Gln Gly Met Phe Ile Gln Ala Asp Phe His Asn
100 105 110
Met Asp Glu Gln Lys Phe Phe Ile Ser Asp Asp Gly Thr Ile Ala Val
115 120 125
Lys Lys Asp Arg Lys Val Trp Asp Val Thr Gly Gly Thr Ser Asn Gly
130 135 140
Ser Pro Ile Ile Pro Tyr Asp Tyr His Gly Ala Ser Asn Gln Lys Phe
145 150 155 160
Thr Leu Glu Arg Ser Gly Ser Val Ser Val Thr Lys Pro Thr Ile Gly
165 170 175
Thr Leu Pro Pro Ala Pro Asp Phe Lys Thr Thr Asp Leu Asn Glu Ile
180 185 190
Leu Pro Asp Asn Thr Glu Pro Val Ile Thr His Ala Thr Tyr Leu Pro
195 200 205
Tyr Phe Met Val Lys Asp Pro Tyr Tyr Asn Asn Gln Gln Lys Met Gln
210 215 220
Asn Ser Pro Tyr Tyr Ile Leu Val Arg Arg Gln Tyr Trp Glu Lys Ile
225 230 235 240
Thr Gln Arg Ile Val Gly Ala Gly Glu Ser Tyr Lys Tyr Glu Gln Lys
245 250 255
Thr Gly Val Ser Arg Thr Asp Gln Thr Ser Met Thr Glu Thr Thr Glu
260 265 270
Ile Ala Ile Gly Ala Asp Met Gly Phe Met Phe Lys Gly Phe Ser Val
275 280 285
Gly Leu Ser Thr Ser Ile Thr Lys Thr Leu Ser Val Thr Lys Ser Thr
290 295 300
Ser Thr Thr Glu Ser Thr Glu Lys Thr Glu Thr Val Thr Asp Ser Asn
305 310 315 320
Pro Phe Met His Thr Ile Ala Arg Ala Lys Tyr Met Leu Ile Asn Glu
325 330 335
Tyr Tyr Val Thr Arg Ala Asn Gly Glu Met Ile Thr Ser Gly Asp Ala
340 345 350
Ala Tyr Trp Lys Val Pro Asn Pro Ser Gln Thr Val Thr Arg Thr Ile
355 360 365
Pro Arg Thr
370
<210> 214
<211> 363
<212> БЕЛОК
<213> Bacillus sp.
<400> 214
Val Ala Phe Asn Leu Gly Ser Tyr Tyr Lys Ile Asn Cys Lys Ile Ser
1 5 10 15
Gly Lys Val Phe Asp Val Arg Asp Glu Ser Leu Asn Asn Gly Ala Tyr
20 25 30
Ile Gln Gln Tyr Thr Trp Lys Asn Ile Asp Gly Gln Lys Phe Met Val
35 40 45
Tyr Pro Leu Asp Asn Gly Thr Tyr Ala Phe Ala Ser Lys Leu Ser Gly
50 55 60
Lys Val Phe Ser Val Arg Ala Ala Ser Met Asp Asn Gln Ala Val Ile
65 70 75 80
Glu Gln Tyr Asp Trp Ser Ala Glu Asp Lys Gln Lys Phe Tyr Leu Thr
85 90 95
Leu Gln Glu Ala Asn Tyr Tyr Ser Gly Thr Ser Gln Leu Ser Gly Lys
100 105 110
Val Trp Asp Val Arg Asp Ser Ser Thr His Asn Gln Ala Leu Ile Gln
115 120 125
Gln Tyr Thr Trp Lys Asp Ile Asp Gly Gln Lys Phe Ser Phe Thr Pro
130 135 140
Val Asp Thr Ile Ser Val Pro Ser Thr Thr Thr Thr Pro Leu Pro Gly
145 150 155 160
Val Pro Glu Tyr His Gly Tyr Asn Asp Gln Leu Pro Asp Gln Thr Tyr
165 170 175
Pro Val Val Thr Ala Tyr Thr Val Ala Pro Phe Phe Ala Ile Ser Asp
180 185 190
Pro Arg Tyr Gly Thr Asn Thr Ala Arg Gln Val Lys Glu Asn Pro Tyr
195 200 205
Tyr Leu Tyr Ile Arg Lys Glu Tyr Trp Lys Leu Leu Gln Ser His Thr
210 215 220
Phe Ala Pro Gly Glu Asp Ala Thr Phe Val Tyr Arg Thr Gly Ile Ser
225 230 235 240
Thr Val Asp Gln Thr Ala Ser Ser Thr Thr Thr Ser Thr Thr Ile Gly
245 250 255
Ala Asp Gly Gly Leu Gln Phe Lys Lys Leu Ser Val Gly Leu Ser Ser
260 265 270
Gln Tyr Thr Thr Glu Leu Gln Thr Ser Gln Ser Thr Thr Asp Ala Glu
275 280 285
Met Lys Glu Gln Glu Val Ala Ile Thr Leu Thr Asn Ile His Pro Tyr
290 295 300
Pro Val Ala Trp Ser Lys Tyr Ile Leu Val Arg Glu Tyr His Val Glu
305 310 315 320
Arg Ser Asp Gly Ser Ile Val Asn Thr Pro Trp Thr Val Ser Leu Pro
325 330 335
Asn Arg Thr Gln Ser Val Tyr Tyr Pro Asn Glu Glu Met Leu Arg Arg
340 345 350
Pro Val Leu Ile Glu Phe Glu Asn Tyr Ser Leu
355 360
<210> 215
<211> 395
<212> БЕЛОК
<213> Bacillus sp.
<400> 215
Val Tyr Gln Val Lys Glu Ile Arg Phe Lys Ile Gly Tyr Asn Ser Phe
1 5 10 15
Ser Phe Cys Ile Glu Glu Ile Tyr Met Met Ser Asn Glu Asn Asn Asn
20 25 30
Arg Arg Asn Met Asn Ala Ser Met Gly Ser Asp Gly Glu Ser Ile Gln
35 40 45
Asn Lys Ile Gly Asp Ser Arg Ser Gln Ile Ser Phe Pro Asp Phe Asn
50 55 60
Phe Tyr Lys Ile Arg Thr Phe Ser Gly Lys Tyr Ala Asp Ile Thr Gly
65 70 75 80
Asp Ser Leu Leu Val Gln Tyr Asn Gly His Asn Gly Asp Asn Gln Lys
85 90 95
Phe Leu Ile Val Thr Leu Asp Asn Asp Tyr Ser Ile Ile Ala Ala Lys
100 105 110
His Thr Gly Lys Val Met His Glu Phe Tyr Leu Phe Gly Thr Gly Arg
115 120 125
Phe Leu Gln Ala Asp Phe His Asn Met Asp Glu Gln Lys Phe Phe Ile
130 135 140
Ser Asp Asp Gly Thr Ile Ala Val Lys Lys Asp Arg Lys Val Trp Asp
145 150 155 160
Val Thr Gly Gly Thr Ser Asn Gly Ser Pro Ile Ile Pro Tyr Asp Tyr
165 170 175
His Gly Ala Ser Asn Gln Lys Phe Thr Leu Glu Arg Ser Gly Ser Val
180 185 190
Ser Val Thr Lys Pro Thr Ile Gly Thr Leu Pro Pro Ala Pro Asp Phe
195 200 205
Lys Thr Thr Asp Leu Asn Glu Ile Leu Pro Asp Asn Thr Glu Pro Val
210 215 220
Ile Thr His Ala Thr Tyr Leu Pro Tyr Phe Met Val Lys Asp Pro Tyr
225 230 235 240
Tyr Asn Asn Gln Gln Lys Met Gln Asn Ser Pro Tyr Tyr Ile Leu Val
245 250 255
Arg Arg Gln Tyr Trp Glu Lys Ile Thr Gln Arg Ile Val Gly Ala Gly
260 265 270
Glu Ser Tyr Lys Tyr Glu Gln Lys Thr Gly Val Ser Arg Thr Asp Gln
275 280 285
Thr Ser Met Thr Glu Thr Thr Glu Ile Ala Ile Gly Ala Asp Met Gly
290 295 300
Phe Met Phe Lys Gly Phe Ser Val Gly Leu Ser Thr Ser Ile Thr Lys
305 310 315 320
Thr Leu Ser Val Thr Lys Ser Thr Ser Thr Thr Glu Ser Thr Glu Lys
325 330 335
Thr Glu Thr Val Thr Asp Ser Asn Pro Phe Met His Thr Ile Ala Arg
340 345 350
Ala Lys Tyr Met Leu Ile Asn Glu Tyr Tyr Val Thr Arg Ala Asn Gly
355 360 365
Glu Met Ile Thr Ser Gly Asp Ala Ala Tyr Trp Lys Val Pro Asn Pro
370 375 380
Ser Gln Thr Val Thr Arg Thr Ile Pro Arg Thr
385 390 395
<210> 216
<211> 412
<212> БЕЛОК
<213> Bacillus sp.
<400> 216
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Thr Ser Asp Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Ser Ile His Leu Gln Ser Asn Asn Asn Lys Val
35 40 45
Trp Asp Val Phe Asn Asn Ala Thr Tyr Asp Asn Ala Gly Ile Arg Leu
50 55 60
Tyr Gly Gln Thr Asn Gly Asp Asn Gln Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Gly Asp Pro Ile Ile Ile Asp Asp Gly Val Arg Arg Met Thr
100 105 110
Ala Phe Asn Asn Glu Gly Leu Arg Gln Thr Ser Trp Thr Gly Ala Pro
115 120 125
Ala Glu Gln Trp Tyr Leu Arg Glu Gln Gly Thr Ser Asn Tyr Glu Phe
130 135 140
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Trp Met Gly Thr
145 150 155 160
Leu Ala Gln Asn Trp Glu Tyr Val Asp Leu Asp Asn Ser Asn Pro Thr
165 170 175
Asp Gly Asp Arg Val Phe Tyr Val Ser Ala Gly Gln Val Asn Lys Gly
180 185 190
Asn Val Pro Phe His Gly Thr Phe Ser Leu Pro Lys Leu Pro Ala Val
195 200 205
Gly Thr Arg Pro Asn Ala Pro Asp Tyr Asn Thr Ser Ala Gly Pro Asn
210 215 220
Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu
225 230 235 240
Val Pro Cys Ile Met Val Asn Asp Asn Gln Ala Ser Asp Tyr Thr Lys
245 250 255
Ile His Asn Ser Pro Tyr Tyr Thr Leu Ile Lys Glu Glu Tyr Trp Asp
260 265 270
Gln Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu Ser Tyr Thr
275 280 285
Phe Lys Ser Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr
290 295 300
Leu Asn Met Lys Ile Gly Ala Asp Phe Gly Leu Gln Phe Gly Ser Lys
305 310 315 320
Thr Pro Ala Thr Pro Gly Thr Pro Glu Ser Ser Asn Gly Gly Thr Val
325 330 335
Ala Ile Lys Ala Glu Ile Ser Arg Thr Leu Gln Thr Glu Ile Ser Thr
340 345 350
Thr Asn Thr Gln Ala Thr Glu Glu Thr Ile Thr Gln Thr Ala Thr Gly
355 360 365
Glu Ala Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys
370 375 380
Tyr Thr Leu Lys Arg Ser Asp Gly Ser Ile Val Ser Asp Pro Trp Val
385 390 395 400
Val Lys Asn Asn Arg Val Thr Val Thr Arg Lys Ile
405 410
<210> 217
<211> 367
<212> БЕЛОК
<213> Неизвестный
<220>
<223> неизвестный организм из образца окружающей среды
<400> 217
Met Lys Asn Lys Ser Ser Lys Ile Val Leu Cys Thr Leu Ser Ala Leu
1 5 10 15
Met Ile Ser Thr Ala Thr Thr Pro Ala Met Ser Ala Phe Ala Ala Glu
20 25 30
Gln Thr Pro Ile Asn Thr Ile Ser Glu Glu Gln Lys Ala Ile Ala Pro
35 40 45
Phe Lys Glu Ser Tyr Ile Asp Thr Val Gln Asn Arg Met Lys Ala Arg
50 55 60
Asp Ala Glu Ser Lys Arg Thr Gly Lys Ala Ile Asn Leu Gln Glu Gln
65 70 75 80
Ile Val Asp Gly Trp Phe Leu Ala Arg Phe Trp Ile Phe Lys Asp Gln
85 90 95
Asn Gly His Gln Ala Asn Arg Phe Ile Ser Trp Phe Lys Ala Asn Leu
100 105 110
Ala Ser Pro Lys Gly Tyr Asp Ser Val Val Glu Gln Met Gly Leu Gln
115 120 125
Met Glu Ala Leu Asp Asp Met Asp Val Ser Asn Val Asn Tyr Ala Ser
130 135 140
Ser Lys Gly Glu Thr Ile Tyr Asn Gly Glu Ser Thr Leu Lys Asn Ile
145 150 155 160
Ser Gly Thr Thr Gln Lys Met Lys Thr Asp Ser Phe Gln Lys Asp Tyr
165 170 175
Thr Gln Thr Gln Ser Thr Ser Val Thr Asn Gly Ile Gln Ile Gly Tyr
180 185 190
Lys Ile Ser Val Ser Ala Asp Val Pro Leu Met Lys Glu Asn Ile Glu
195 200 205
Ile Ser Thr Thr Tyr Ile Leu Ser Thr Thr Gly Thr Glu Thr Asn Thr
210 215 220
Ile Ser Glu Lys Tyr Asn Val Pro Ser Gln Glu Val Leu Val Pro Pro
225 230 235 240
Gly His Thr Ala Val Val Lys His Asp Leu Arg Lys Met Val Tyr Ser
245 250 255
Gly Thr His Asp Leu Lys Gly Asp Leu Asn Val Arg Phe Asp Asp Lys
260 265 270
Glu Ile Val Gln Lys Phe Val Tyr Pro Asn Tyr Lys Pro Ile Asn Leu
275 280 285
Ser Asp Ile Arg Ser Thr Met Asn Ala Ile Asp Thr Trp Asn Gly Ile
290 295 300
Lys Pro Val Asp Phe Tyr Gln Leu Leu Gly Ile Lys Asn His Ile Lys
305 310 315 320
Gly Ser Asn Thr Leu Tyr Ile Asp Thr Pro Ala Lys Phe Lys Phe Asn
325 330 335
Gly Ala Ser Pro Tyr Tyr Arg Ala Thr Ile Thr Glu Tyr Asp Lys Asn
340 345 350
Gly Asn Pro Val Glu Thr Arg Leu Leu Asp Pro Asn His Gln Leu
355 360 365
<210> 218
<211> 367
<212> БЕЛОК
<213> Bacillus sp.
<400> 218
Met Glu Phe Lys Pro Gly Lys Met Tyr Arg Phe Lys Asn Lys Met Ser
1 5 10 15
Gly Lys Tyr Leu Asp Val Ala Arg Asp Ser Arg Asp Glu Trp Ala Asn
20 25 30
Ile Gln Gln Tyr Glu Tyr Val Lys Asp Ser Leu Ser Glu Lys Phe Phe
35 40 45
Ile His Pro Leu Asp Asn Asn Tyr Tyr Ala Ile Ile Ser Cys Leu Ser
50 55 60
Gly Arg Thr Leu Asp Ile Ala Tyr Asp Ser Lys Asp Asn Trp Ala Asn
65 70 75 80
Val Gln Leu Tyr His Trp Thr Gly Ser Asp Ser Glu Thr Trp Ser Leu
85 90 95
Asn Ala Asp Pro Thr Asn Pro Asp Tyr His Val Val Leu Ser Asn Leu
100 105 110
Ser Gly Lys Val Leu Asp Val Ala Arg Asp Ser Thr Asp Asn Trp Ala
115 120 125
Asn Ile Gln Met Tyr Glu Glu Lys Pro Asp Leu Asp Gly Gln Lys Trp
130 135 140
Leu Ile Glu Glu Asp Gly Glu Val Thr Leu Pro Thr Thr Glu Thr Glu
145 150 155 160
Ala Leu Pro Ser Val Pro Glu Cys Thr Lys Ile Asn Glu Val Leu Pro
165 170 175
Asp Lys Thr Lys Lys Val Ala Thr His Thr Thr Thr Ile Pro Ala Ile
180 185 190
Leu Val Gln Asp Pro Ile Tyr Ser Leu Ala Asp Gln Ile Lys Lys Ser
195 200 205
Pro Tyr Tyr Asn Ile Val Lys Asn Gln Tyr Trp Arg Lys Ala Ala Asp
210 215 220
Tyr Arg Met Thr Pro Gly Glu Ser His Glu Val Thr Asn Thr Thr Gly
225 230 235 240
Ile Thr Glu Thr Asp Gln Gln Thr Ile Lys His Thr Leu Gly Ile Ser
245 250 255
Ile Gln Gln Asp Met Gly Ile Ser Phe Glu Gly Val Ser Ala Ser Ser
260 265 270
Ser Arg Gln Phe Lys Tyr Glu Leu Glu Ile Glu Asn Ser His Thr Thr
275 280 285
Glu Lys Leu Lys Gln Glu Thr Gln Arg His Lys Ile Val Asn Ile Arg
290 295 300
Asp Thr Asp Met Arg Phe Val Gln Tyr Ile Leu Glu Thr Glu Tyr Thr
305 310 315 320
Leu Lys Arg Ser Asn Gly Ser Ile Val Lys Thr Trp Ser Leu Asn Asp
325 330 335
Lys Asn Thr Val His Ser Val Ser Tyr Phe Gln Lys Asp Ser Glu Asn
340 345 350
Thr Ser Val Val Ala Arg Ile Ser Asp Ile Glu Thr Ile Ser Ser
355 360 365
<210> 219
<211> 412
<212> БЕЛОК
<213> Bacillus sp.
<400> 219
Leu Ser Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys Lys
1 5 10 15
Val Met Ala Gly Leu Leu Val Gly Ala Thr Ser Leu Ser Ile Trp Ala
20 25 30
Pro Ile Ser Glu Ala Ala Ala Pro Glu Asn Asn Arg Tyr Tyr Asn Ile
35 40 45
Ala Leu Lys Ser Asn Thr Lys Lys Val Trp Asn Val Ser Gln Gly Ser
50 55 60
Asn Asp Asn Asp Arg Ala Ile Val Leu Trp Gln Gly Gly Ser Ala Asp
65 70 75 80
His Glu Arg Phe Ala Phe Phe Gln Leu Asp Gly Gly Ala Tyr Ala Ile
85 90 95
Val Asn Lys Asn Ser Gly Lys Val Val Thr Phe Gly Thr Gly Ser Trp
100 105 110
Val Ser Gly Gly Pro Ile Pro Asp Ser Glu Thr Leu Gln Gln Gln Lys
115 120 125
Trp Thr Gly Glu Ala Lys Gln Lys Trp Asn Leu Arg Asp Leu Gly Ser
130 135 140
Asp Gly His Gly Ser Asn Asn Tyr Glu Ile Val Asn Gln Ala Asn Gly
145 150 155 160
Lys Val Pro Ser Tyr Arg Trp Ile Gly Ser Thr Val Asp Asn Thr Glu
165 170 175
Tyr Leu Lys Leu Asp Asn Ser Asn Pro Ser Asp Ala Asp Lys Val Phe
180 185 190
Thr Leu Phe Asn Gly Thr Met Asn Leu Trp Gly Gln Asn Ile Thr Phe
195 200 205
Phe Asn Asp Thr Ile Ser Val Pro Lys Leu Pro Ala Ile Gly Thr Arg
210 215 220
Pro Glu Ala Pro Val Tyr Thr Gly Gly Ala Asp Gln Gln Leu Pro Gln
225 230 235 240
Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro Cys Phe Met
245 250 255
Val Asn Asp Asn Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro
260 265 270
Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ala
275 280 285
Val Ile Pro Gly Gly Leu Lys Arg Asp Tyr Thr Phe Lys Ser Gly Met
290 295 300
Thr Ser Val Asp Gln Lys Lys Met Thr Asp Thr Leu Ser Met Lys Ile
305 310 315 320
Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys
325 330 335
Thr Glu Ile Ser Arg Thr Leu Gln Thr Glu Ile Thr Ser Thr Asp Thr
340 345 350
Glu Val Ser Glu Glu Thr Val Ser Ser Ser Leu Thr Ser Pro Ala Gly
355 360 365
Lys Thr Thr Gly Phe Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu
370 375 380
Lys Arg Ala Asp Gly Ser Ile Val Ser Asp Pro Trp Ile Val Lys Asn
385 390 395 400
Asn Lys Ile Ala Ile Val Arg Asp Lys Val Gln Gly
405 410
<210> 220
<211> 412
<212> БЕЛОК
<213> Bacillus sp.
<400> 220
Val Arg Arg Asn Asn Met Lys Asn Ser Pro Tyr Lys Ile Leu Ser Ala
1 5 10 15
Thr Ala Met Ile Ala Ala Ile Thr Val Gly Asn Ile Ile Pro Ser Thr
20 25 30
Pro Val Phe Ala Gln Glu Gln Val Val His Glu Gln Gln Asn Ser Ala
35 40 45
Tyr Ala Leu Gly Pro Asn Gly Met Lys Asp Ala Leu Ala Lys Thr Gly
50 55 60
Ser His Met Leu Val Met Asn Val Tyr Ala Ser Thr Met Ile Lys Gln
65 70 75 80
Pro Thr Val Asn Leu Ser Gly Ile Asp Leu Gly Ala Asp Gly Glu Gln
85 90 95
Leu Val Lys Lys Ile Gln Asn Asp Gln Asn Val Ser Ile Ser Asn Ala
100 105 110
Asn His Trp Met Asp Val Glu Lys Pro Lys Ile Gln Lys Ala Ala Arg
115 120 125
Ser Ile Val Asn Tyr Asn Thr Gln Phe Gln Asn Tyr Tyr Gly Thr Ile
130 135 140
Val Gly Ala Ala Lys Gln Asn Asn Thr Pro Thr Leu Lys Glu Gly Leu
145 150 155 160
Gln Asp Leu Tyr Thr Thr Ile Asn Lys Asn Ser Lys Glu Val Asp Asp
165 170 175
Val Ile Lys Ala Leu Lys Glu Phe Lys Asn Lys Leu Tyr Thr Asp Ser
180 185 190
Thr Gln Phe Lys Asn Asp Val Asp Gly Ala Asp Gly Lys Ala Gly Leu
195 200 205
Ala Thr Ile Leu Ala Gly Lys Asn Ala Leu Ile Pro Gln Leu Lys Ser
210 215 220
Glu Ile Glu Ser Leu Arg Gly Ser Gln Lys Ala Tyr Leu Asp Asn Val
225 230 235 240
Leu Gly Trp Gly Ile Gly Gly Gly Val Gly Thr Phe Leu Leu Ile Gly
245 250 255
Gly Ala Ile Gly Gly Ala Val Ala Ile Val Val Thr Gly Gly Thr Ala
260 265 270
Thr Pro Leu Ile Val Gly Gly Leu Val Ala Leu Gly Ala Ala Gly Ile
275 280 285
Gly Leu Gly Thr Ala Thr Gly Val Met Leu Ser Gln Asn Leu Thr Gln
290 295 300
Tyr Asn Asp Val Ser Lys Lys Ile Glu Gln Leu Ser Thr Gln Arg Thr
305 310 315 320
Ala Ala Glu Gln Ala Val Leu Ser Leu Ala Asn Ala Lys Ala Thr Leu
325 330 335
Met Asp Leu Tyr Gln Thr Val Asp Gln Ala Ile Asp Ser Leu Thr Ala
340 345 350
Met Lys Lys Gln Trp Asp Thr Met Gly Ala Asn Tyr Lys Val Leu Leu
355 360 365
Asp Ser Val Asp Ser Met Gln Pro Ser Lys Val Ser Leu Leu Val Asn
370 375 380
Asp Leu Asp Ala Ala Lys Gln Gln Trp Ser Asp Ile Tyr Gln Ala Ala
385 390 395 400
Asp Leu Val Ala Lys Asp Ile Gly Phe Lys Asn Glu
405 410
<210> 221
<211> 382
<212> БЕЛОК
<213> Bacillus sp.
<400> 221
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ala Ser Glu Ala Ala Pro Ala Asn
20 25 30
Asn Ala Tyr His Ser Ile His Leu Ala Ala Asp Ala Thr Leu Lys Trp
35 40 45
Asp Val Tyr Gly Asn Gly Thr Tyr Asp Glu Ala Gly Ile Leu Leu Tyr
50 55 60
Asn Gly Asn Ile Ser Asp Asn Glu Gln Phe Leu Phe Phe Pro Leu Asp
65 70 75 80
Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Ala Val Thr
85 90 95
Ile Gly Gly Gly Trp Val Gly Asp Asn Ala Phe Ser Tyr Asn Arg Glu
100 105 110
Gly Leu Ile Gln Ser Lys Trp Thr Gly Ala Asn Thr Asn Lys Trp Tyr
115 120 125
Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly Tyr
130 135 140
Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile Ser Gly Ala Lys Tyr
145 150 155 160
Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn Asp Arg Leu Phe Asn
165 170 175
Ile Pro Ala Thr Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu Pro Asn
180 185 190
Val Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Ile Asp Gln
195 200 205
Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Leu
210 215 220
Pro Cys Ile Met Val Asn Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile
225 230 235 240
His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Arg
245 250 255
Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu Ser Tyr Ser Tyr
260 265 270
Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu
275 280 285
Ser Met Lys Phe Gly Ala Asp Leu Gly Ile Lys Phe Gly Asp Ala Ser
290 295 300
Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile Ser
305 310 315 320
Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr Thr Ala Thr
325 330 335
Gly Glu Ser Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr
340 345 350
Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val Ser Asp Pro Trp
355 360 365
Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys Thr Ser
370 375 380
<210> 222
<211> 318
<212> БЕЛОК
<213> Bacillus sp.
<400> 222
Met Lys Lys Lys Met Lys Lys Pro Met Val Ala Leu Met Leu Ala Thr
1 5 10 15
Ser Ile Gly Ile Pro Cys Ile Ser Val Pro Gly Ser Ala Leu Ala Ala
20 25 30
Glu Asn Thr Pro Thr Ser Val Asn Glu Asn Val Arg Val Gly Ile Thr
35 40 45
Asp Val Gln Ser Glu Leu Lys Lys Ile Gly Thr Tyr Tyr Tyr Thr Asn
50 55 60
Glu Ile Ala Gly Thr Lys Ile Glu Ser Trp Pro Thr Leu Thr Phe Lys
65 70 75 80
Ala Lys Pro Asp Ser Val Glu Thr Lys Val Asp Phe Ser Ile Thr Asp
85 90 95
Thr Thr Asn Lys Leu Asn Phe Asp Ser Lys Ile Val Glu Tyr Met Gly
100 105 110
Glu Asn Val Phe Glu Asn Thr Thr Asp Gln Thr His Lys Phe Ser Thr
115 120 125
Ala Lys Tyr Thr Lys Ser Val Thr Glu Ser Ile Ala Thr Ala Thr Thr
130 135 140
Lys Gly Phe Asn Val Gly Gly Ser Ser Asn Ile Phe Asn Ile Pro Leu
145 150 155 160
Ile Leu Asn Asn Gly Ile Thr Val Asn Ala Gly Phe Asn Ser Ser Thr
165 170 175
Thr Glu Thr Gln Thr Lys Ala Glu Gln Lys Thr Ile Glu Ala Pro Ser
180 185 190
Gln Ser Ile Glu Val Pro Pro Gln Lys Thr Tyr Lys Ala Asp Val Ile
195 200 205
Leu Glu Gln Arg Asn Phe Trp Gly Asp Ile Thr Phe Thr Gly Val Gly
210 215 220
Ser Asn Pro Val Thr Thr Ile Lys Gly Thr Ala Ser Tyr Trp Ala Pro
225 230 235 240
Asn Gly Met Gly Ala Trp Lys Gln Tyr Pro Phe Ser Asp Lys Thr Lys
245 250 255
Asn Tyr Trp Asn Lys Leu Thr Thr Ser Gln Lys Asn Asn Leu Asn Gly
260 265 270
Ile Lys Phe Glu Asn Asn Asp Val Lys Ala Glu Gly Thr Ala Lys Val
275 280 285
Glu Gly Ile Phe Gly Ser Asn Leu Ile Val Lys Val Tyr Asp Ile Thr
290 295 300
Asp Lys Ser Asn Pro Lys Leu Val Glu Thr Arg Ser Phe Lys
305 310 315
<210> 223
<211> 260
<212> БЕЛОК
<213> Bacillus sp.
<400> 223
Met Tyr Pro Asp His Gln Gln Trp Ile Ile Tyr Leu Leu Asn Asn Gly
1 5 10 15
Asn Ile Ile Leu Tyr Cys Lys Asn Ser Gly Leu Asn Leu Asp Asn Gln
20 25 30
Gly Gln Thr Lys Asp Gly Thr Pro Val Thr Thr Tyr His Trp Thr Gly
35 40 45
Thr Asp His Gln Leu Trp Lys Ile Glu Glu Tyr Ser Asp Val Asp Met
50 55 60
Pro Ser Ile Thr Thr Ser Thr Leu Glu Pro Ala Pro Glu Phe Thr Asn
65 70 75 80
Ala Asp Thr Leu Pro Pro Thr Ala Lys Pro Val Val Thr Asn Tyr Ala
85 90 95
Leu Ile Pro Cys Val Met Val Thr Asp Pro Phe Gly Trp Asn Tyr Gln
100 105 110
Thr Lys Met Gln Lys Asn Pro Tyr Tyr Ala Phe Thr Lys Ala Glu Ser
115 120 125
Trp Glu Leu Gln Ala Ala Leu Tyr Asn Val Ser Ser Gly Asp Glu Arg
130 135 140
Asp Tyr Glu Ile Thr Glu Gly Ile Ser Gln Glu Asp Gln Gln Ser Met
145 150 155 160
Ser Lys Thr Thr Ser Ile Thr Ile Gly Ala Asp Tyr Gly Phe Ser Phe
165 170 175
Gly Pro Leu Ser Thr Ser Ile Ser Ala Ser Phe Thr Glu Glu Leu Gln
180 185 190
Val Ser Glu Ser His Ser Asn Thr Glu Ser Val Glu Val Thr Lys Thr
195 200 205
Leu Lys His Asn Asn Ala Thr Asp Ser Ser Glu Ser Trp Ala Leu Tyr
210 215 220
Tyr Arg Glu Phe Ser Tyr Asn Leu Gln Arg Leu Asp Gly Ser Ser Thr
225 230 235 240
Thr Asp Ala Trp Thr Val Leu Asp Ser Thr Lys Thr Ser Glu Thr Ser
245 250 255
Trp Pro Gln Ala
260
<210> 224
<211> 301
<212> БЕЛОК
<213> Bacillus sp.
<400> 224
Met Glu Arg Arg Leu Ile Cys Met Ala Ile Val Asp Leu Asp Ala Tyr
1 5 10 15
Leu Val Glu Leu Phe Lys Lys Ile Met Tyr Pro Ser Pro Thr Arg Glu
20 25 30
Asp Thr Thr Asn Leu Thr Val Lys His Ala Glu Ser Tyr Gly Phe Glu
35 40 45
Ile Lys Ala Asn Lys Pro Gln Gly Thr Leu Phe Met Gly Glu Ala Val
50 55 60
Leu Glu Asn Lys Thr Asn Glu Thr Gln Thr Leu Lys Ser Asp Ser Phe
65 70 75 80
Thr Lys Thr Val Thr Asp Ser Ile Thr Leu Ser Val Ile Asn Gly Ile
85 90 95
Lys Thr Gly Val Asn Ile Gly Ile Ser Gly Lys Val Phe Gly Ile Gly
100 105 110
Val Glu Ser Ser Met Ser Phe Glu Val Ser Thr Ser Thr Thr Arg Glu
115 120 125
Gln Thr Ser Thr Glu Gly Val Lys Tyr Thr Val Pro Ser Gln Asn Val
130 135 140
Val Val Pro Ala Lys Lys Thr Tyr Tyr Val Tyr Thr Ser Leu Gln Arg
145 150 155 160
Ser Asn Leu Glu Gly Thr Val Arg Leu Arg Ala Asp Leu Ser Gly Pro
165 170 175
Ile Leu Ile Met Leu Lys Phe Gly Ser Thr Glu Ile Pro Val Tyr Ala
180 185 190
Gly Asp Ile Phe Glu Thr Leu Asn Gly Tyr Gln Phe Leu His Pro Leu
195 200 205
Pro Ser Gly Ile Ser Leu Asn His Lys Asp Lys Ser Ile His Phe Glu
210 215 220
Gly Ile Ala Glu Tyr Ile Tyr Gly Thr Gly Thr Lys Phe Tyr Val Thr
225 230 235 240
Val Thr Asp Thr Pro Ser Ser Gln Gly Thr Leu Glu His Lys Pro Phe
245 250 255
Asp Ala Lys Thr Gly Leu Gly Thr Tyr Glu Leu Leu Leu Asn Gly Lys
260 265 270
Lys Pro Val Leu Asp Leu Asn Asp Leu Lys Glu His Leu Glu Pro Lys
275 280 285
Asp Phe Glu Lys Leu Lys Lys Leu Gln Asn Glu Ile Val
290 295 300
<210> 225
<211> 313
<212> БЕЛОК
<213> Bacillus sp.
<400> 225
Met Lys Met Ala Ile Thr Asp Ile Glu Ser Ala Ala Lys Asp Tyr Met
1 5 10 15
Asn Trp Ala Leu Pro His Tyr Ser Gly Tyr Ser Pro Thr Ser Tyr Lys
20 25 30
Phe Tyr Pro Tyr Thr Ile Ser Asn Val Ser Ala Val Pro Ala Gln Thr
35 40 45
Ala Phe Glu Thr Ala Pro Val Gln Lys Ala Ala Ser Val Gln Val Leu
50 55 60
Thr Asn Asn Thr Ser Leu Thr Gln Ser Gln Thr Leu Lys Phe Ser Glu
65 70 75 80
Lys Leu Ile Glu Ser Thr Thr Gly Ser Thr Thr Glu Gly Tyr Lys Ile
85 90 95
Gly Ser Gly Ile Thr Ser Thr Ser Lys Phe Ser Tyr Asn Val Lys Ile
100 105 110
Phe Ala Ala Asn Ile Gly Phe Asp Gln Thr Phe Thr Val Thr Thr Thr
115 120 125
Gly Glu Tyr Asn His Ser Ser Thr Glu Thr Thr Thr Lys Thr Thr Glu
130 135 140
Lys Leu Trp Glu Val Thr Gln Pro Val Thr Val Pro Pro Tyr Thr Gln
145 150 155 160
Ile Val Ala Thr Leu Ile Ile Leu Gly Ala Asp Leu Gln Ile Pro Met
165 170 175
Arg Leu Asn Ala Thr Leu Ile Gly Asn Gly Thr Glu Phe Gly Ala Glu
180 185 190
Asn Leu Tyr Cys Ala Ala Tyr Phe Tyr Asn Pro Asn Tyr Met Val Gln
195 200 205
Pro Tyr Tyr Ala Gly Asn Met Ala Asp Arg Ala Trp Pro Asn Arg Pro
210 215 220
Ala Ser Phe Ile Gln Gly Val Tyr Ser Leu Val Leu Gln Gly Ala Ser
225 230 235 240
Thr Thr Ser His Gly Pro Gly Leu Tyr Ala Ala Val Leu Phe Glu Gln
245 250 255
Thr Pro Leu Pro Gly Phe Glu Thr Phe Ser Glu Ser Lys Thr Trp Tyr
260 265 270
Ser Asn Glu Val Leu Leu Arg Asp Gly Ser Ile Ile Thr Ile Pro Asp
275 280 285
Leu Asp Pro Lys Thr Gly Arg Ala Leu Pro Ala Lys Val Leu Glu Pro
290 295 300
Val Met Val Ser Arg Ser Ser Asn Asp
305 310
<210> 226
<211> 449
<212> БЕЛОК
<213> Bacillus sp.
<400> 226
Leu Val Leu Ser Phe Tyr Ile Ala Lys Val Ile Leu Phe Arg Asn Lys
1 5 10 15
Ser Ile Ser Asn Val Gln Tyr Asn Phe Ile Phe Cys Asn Leu Val Gly
20 25 30
Ile Leu Pro Thr Lys Leu Gly Gly Asn Ile Met Lys Ser Ile Ser Lys
35 40 45
Lys Val Met Ala Gly Leu Gly Val Gly Val Met Ser Leu Ser Ile Trp
50 55 60
Ala Pro Ser Ser Gln Ala Ala Ala Pro Glu Asn Asn Arg Tyr Tyr Ser
65 70 75 80
Ile His Leu Lys Ala Asn Pro Lys Leu Ala Trp Asp Val Tyr Lys Gly
85 90 95
Trp Asp Ser Asp Asn Ala Gly Ile Val Leu Tyr Asn Gly Ser His Gly
100 105 110
Asp Asn Glu Gln Phe Thr Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala
115 120 125
Ile Val Asn Lys Asn Ser Gly Lys Pro Val Gly Leu Gly Asn Ala Tyr
130 135 140
Ile Ser Thr Asp Gly Val Arg Asp Gly Phe Ser Gly Asn Asn Gln Gly
145 150 155 160
Leu Arg Gln Thr Asn Trp Thr Gly Ala Ser Asn Glu Gln Trp Tyr Leu
165 170 175
Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly Thr Gly
180 185 190
Lys Val Ala Ser Trp Ser Trp Asn Gly Ile Ser Gly Pro His Ser Ala
195 200 205
Glu Tyr Val Asp Leu Asp Glu Ser Asn Pro Ser Asp Asn Asn Arg Val
210 215 220
Phe Asn Ile Ala Lys Asn Gly Leu Gly Gly Ile Gly Val Ser Ile Pro
225 230 235 240
Gly Phe Asp Ile Asp Tyr Gly Thr Phe Ser Leu Pro Thr Leu Pro Ala
245 250 255
Val Gly Thr Arg Pro Asn Ala Pro Asp Tyr Asn Thr Ser Gly Ala Ile
260 265 270
Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser
275 280 285
Leu Ile Pro Ser Ile Met Val Lys Asp Asn Gly Ala Ser Asp Tyr Thr
290 295 300
Lys Ile His Asn Ser Pro Tyr Tyr Ala Leu Val Lys Glu Glu Tyr Trp
305 310 315 320
Glu Lys Val Arg Ser Glu Ile Ile Pro Ala Gly Ala Thr Ser Lys Tyr
325 330 335
Thr Val Lys Ser Gly Val Ser Glu Ile Asp Gln Lys Lys Met Ser Asp
340 345 350
Thr Leu Ala Met Ser Phe Gly Ser Asp Leu Gly Leu Lys Phe Gly Glu
355 360 365
Ser Ser Leu Ser Ile Lys Ser Ser Val Ser Arg Thr Ile Gln Thr Glu
370 375 380
Val Thr Lys Thr Ser Thr Asn Ala Thr Glu Glu Thr Glu Glu Lys Ser
385 390 395 400
Ile Pro Ser Ile Pro Gly Lys Asn Thr Gly Phe Thr Ala Tyr Gln Leu
405 410 415
Val Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly Ser Val Val Thr Asp
420 425 430
Pro Ser Ile Val Arg Asp Ser Lys Gln Thr Ile Ala Arg Thr Ile Ala
435 440 445
Asn
<210> 227
<211> 438
<212> БЕЛОК
<213> Bacillus sp.
<400> 227
Val Ile Leu Phe Arg Asn Lys Ser Ile Ser Asn Val Gln Tyr Asn Ile
1 5 10 15
Ile Phe Cys Asn Leu Val Gly Ile Leu Pro Thr Lys Leu Gly Gly Asn
20 25 30
Ile Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Ala Val Gly
35 40 45
Ala Met Ser Leu Ser Ile Trp Ala Pro Ala Ser Gln Ala Asp Ser Pro
50 55 60
Glu Lys Asn Arg Tyr Tyr Ser Ile His Leu Lys Ser Asn Pro Lys Leu
65 70 75 80
Ala Trp Asp Val Asn Asp Asn Ser Pro Asp Asp Asn Arg Ala Ile Leu
85 90 95
Leu Trp Asn Thr Ser Ser Arg Gly Asp Asn Glu Gln Phe Val Phe Phe
100 105 110
Pro Leu Asp Gly Gln Asn Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys
115 120 125
Pro Val Thr Phe Gly Gly Ser Ser Pro Ala Tyr Ile Gly Asp Arg Asp
130 135 140
Ala Leu Leu Ser Asn Arg Glu Ala Leu Gln Gln Arg Ser Trp Thr Gly
145 150 155 160
Ser Pro Thr Glu Gln Trp Tyr Leu Arg Asp Lys Gly Ser Asn Asn Tyr
165 170 175
Glu Ile Val Asn Gln Gly Asn Gln Lys Val Ala Ser Tyr Ala Tyr Thr
180 185 190
Trp Ala Ser Arg His Val Glu Tyr Val Asp Leu Asp Glu Ser Asn Pro
195 200 205
Ser Asp Ser Asp Lys Val Phe Asn Ile Ala Lys Asn Gly Leu Gly Gly
210 215 220
Leu Gly Val Ser Ile Pro Gly Phe Asp Ile Asp Tyr Gly Thr Phe Ser
225 230 235 240
Leu Pro Thr Leu Pro Ala Thr Gly Thr Arg Pro Thr Ala Pro Glu Tyr
245 250 255
Asn Lys Asp Ala Ala Asp Pro Asn Gln Gln Leu Pro Gln Thr Ser Asn
260 265 270
Ser Val Val Val Gly Ala Ser Leu Val Pro Cys Ile Met Val Thr Asp
275 280 285
Ser Gln Thr Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr
290 295 300
Leu Ile Lys Glu Glu Tyr Trp Asp Lys Ala Ala Ser Thr Val Leu Ala
305 310 315 320
Pro Gly Ser Thr Gln Ser Val Ala Phe Lys Thr Gly Met Ser Ser Ser
325 330 335
Asp Gln Gln Lys Met Thr Glu Thr Ile Ser Met Lys Ile Gly Ala Asp
340 345 350
Phe Gly Leu Lys Phe Leu Asp Lys Ile Gly Pro Leu Lys Ala Glu Ile
355 360 365
Ser Lys Thr Leu Gln Thr Glu Thr Ser Thr Ser Asn Thr Glu Leu Ser
370 375 380
Glu Asp Thr Val Thr Thr Asn Leu Thr Gly Glu Ala Gly Lys Thr Thr
385 390 395 400
Gly Tyr Thr Leu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala
405 410 415
Asp Gly Ser Glu Val Ser Gly Ser Trp Thr Val Lys Asn Asn Lys Ile
420 425 430
Thr Lys Ile Ser Lys Ser
435
<210> 228
<211> 604
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 228
Met Val Thr Lys Ala Val Thr Pro Lys Leu Gly Ala Leu Pro Asp Phe
1 5 10 15
Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Ser Gly Ile Thr Asp
20 25 30
Val Met Ser Thr Ile Phe Gly Val Asn Thr Gly Asp Ser Ser Ile Asp
35 40 45
Asn Val Met Asn Asn Glu Ala Leu Leu Lys Gln Met Met Asp Gln Met
50 55 60
Asn Ala Met Gln Ser Ser Leu Asp Asp Ile Leu Lys Asn Gln Lys Met
65 70 75 80
Ser Glu Val Leu Gln His Met Leu Val Asn Leu Ser Asn Glu Gln Leu
85 90 95
Asp Leu Ser Lys Ser Ile Asn Val Glu Leu Gly Lys Ile Glu Gly Ile
100 105 110
Leu Thr Thr Tyr Leu Pro Ala Ile Ser Ser Met Leu Asn Asn Ile Tyr
115 120 125
Ser Gln Thr Ser Glu Ile Asn Gln Lys Val Asp Lys Leu Leu Ala Leu
130 135 140
Met Thr Phe Ala Leu Gln Glu Leu Glu Tyr Ile Lys Asp Asn Val Ile
145 150 155 160
Leu Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu Val
165 170 175
Tyr Val Asn Lys Lys Phe Leu Glu Leu Ser Gly Met Tyr Met Ser Gly
180 185 190
Asp Ile Ser Ile Asp Asn Met Gln Glu Leu Ile Gln Trp Ala Lys Ser
195 200 205
Ile Val Asp Thr Asp Met Asn Ser Phe Glu Phe Ser Ile Asp Ser Leu
210 215 220
His Asn Thr Ile Val Gly Asp Asn Leu Tyr Lys Arg Ser Ala Leu Lys
225 230 235 240
Thr Phe Ala Asp Val Leu Met Asp Asp Asp Ser Lys Tyr Gly Asp Phe
245 250 255
Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu Ala Thr
260 265 270
Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Val Ala Arg Lys Val Leu
275 280 285
Gly Leu Thr Asp Ile Asn Tyr Ser Leu Thr Met His Glu Arg Met Glu
290 295 300
Gln Gln Asn Gln Leu Phe Leu Asn Ile Ile Gln Asp Gln Lys Ile Ser
305 310 315 320
Asn Tyr Leu Lys Ile Lys Gly Thr Tyr Ser Met Asp Ser Gln Tyr Val
325 330 335
Asp Glu Leu Lys Thr Ser Asp Leu Gln Ala Lys Asp Gly Tyr Ala Leu
340 345 350
Ile Gly Leu Glu Phe Phe Val Asp Asn Gly Ile Tyr Lys Ala Lys Ala
355 360 365
Tyr Gln Gly Lys Ile Asp Thr Asn Phe Ser Val His Ala Asn Thr Leu
370 375 380
Glu Glu Ile Val Ser Asp Asp Leu Ser Glu Val Phe Pro Glu Gly Ile
385 390 395 400
Arg Lys Asp Gly Thr Ser Val Arg Tyr Pro Leu Ser Gly Asn Leu Ala
405 410 415
Gly Pro Asp Asn Ser Val Ile Thr Arg Ile Gly Phe Gly Ser Lys Tyr
420 425 430
Val Ser Lys Asp Asp Cys Tyr Ala Tyr Ile Glu Ala Asp Phe Ser Pro
435 440 445
Tyr Asp Pro Ser Ser Gly Gln Val Met Lys Glu Gly Thr Gln Thr Ile
450 455 460
Ser Leu Gln Gly Asn Lys Gly Thr Thr Ile Ala Tyr Asp Thr Trp Pro
465 470 475 480
Ile Gly Leu Ile Ser Asp Phe Tyr Met Ser Pro Leu Lys Asn Leu Ser
485 490 495
Leu Tyr Val Asp Val Asn Glu Asn His Gly Tyr Ser Val Met Asn Phe
500 505 510
Gly Gly Glu Ser Tyr Tyr Tyr Thr Met Leu Ser Arg Glu Tyr Asp Ala
515 520 525
Asn Phe Ile Leu Val Ser Tyr Thr Glu Asn Gly Ser Arg Lys Pro Glu
530 535 540
Asn Leu Ile Glu Asn Gly Asp Phe Glu Asn Lys Gly Asp Gln Trp Asp
545 550 555 560
Phe Lys Thr Tyr Gly Gln His Asp Lys Pro Tyr Val Ala Glu Gly Ile
565 570 575
Gly Leu Asp Gly Ser Asn Ala Leu Ile Ile Glu Asp Gly Ile Ala Leu
580 585 590
Gln Tyr Leu Asn Leu Lys Pro Tyr Thr Asn Ile Asn
595 600
<210> 229
<211> 672
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 229
Met Ser Ser Asn Asn Gln Asn Asn Leu Asp Ile Val Ser Thr Ser Glu
1 5 10 15
Lys Lys Phe Val Ser Lys Glu His Ser Lys Asn Leu Leu Thr Asn Asp
20 25 30
Leu His Asn Met Asn Tyr Lys Asp Phe Leu Arg Met Ser Asp Glu His
35 40 45
Asn Ala Glu Phe Leu Ala Asn Ala Glu Val Phe Val Asn Thr Gln Asp
50 55 60
Ser Ile Lys Leu Gly Met Asn Ile Val Gly Glu Ile Leu Ser Ser Leu
65 70 75 80
Gly Val Pro Phe Ile Gly Pro Ile Val Ser Phe Tyr Thr Thr Ile Ile
85 90 95
Asp Leu Leu Trp Pro Ser Ser Gly Gly Lys Asn Pro Trp Glu Ile Phe
100 105 110
Met Glu Gln Val Glu Glu Leu Met Asp Gln Lys Ile Ala Glu Tyr Ala
115 120 125
Arg Lys Lys Ala Leu Ala Glu Leu Glu Gly Leu Gly Ala Asn Phe Glu
130 135 140
Leu Tyr Arg Ile Ala Leu Glu Glu Trp Lys Glu Asn Pro Asn Ile Ser
145 150 155 160
Arg Thr Thr Arg Asp Val Arg Thr Arg Phe Glu Ile Leu Asp Ser Leu
165 170 175
Phe Thr Gln Tyr Met Pro Ser Phe Lys Val Ser Gly Tyr Glu Val Pro
180 185 190
Leu Leu Thr Val Tyr Thr Gln Ala Ala Asn Leu His Leu Leu Leu Leu
195 200 205
Arg Asp Ala Ser Ile Phe Gly Glu Glu Trp Gly Leu Ser Thr Thr Thr
210 215 220
Ile Asn Asn Tyr Tyr Lys Arg Gln Ile Asn Leu Thr Ala Gln Tyr Ser
225 230 235 240
Asp His Cys Val Lys Trp Tyr Asn Thr Gly Leu Asp Arg Leu Lys Gly
245 250 255
Ser Asn Ala Glu Ser Trp Leu Lys Tyr His His Phe Arg Arg Glu Met
260 265 270
Thr Leu Met Val Leu Asp Ile Val Ala Leu Tyr Pro Asn Tyr Asp Thr
275 280 285
His Thr Tyr Pro Ile Glu Thr Lys Ala Gln Leu Thr Arg Glu Val Tyr
290 295 300
Thr Asp Pro Leu Gly Tyr Asn Pro Thr Asn Pro Glu Asp Asn Gly Phe
305 310 315 320
Gly Ile Pro Trp Thr Arg Leu Ser Phe His Asn Phe Ala Gln Ile Glu
325 330 335
Lys Asn Ala Ile Arg Pro Pro His Leu Phe Asp Ile Leu Lys Ser Val
340 345 350
Glu Ile Ser Thr Ile Lys Gly Thr Leu Ala Leu Asn Asn Lys Ala Tyr
355 360 365
Ile Asn Phe Trp Ser Gly His Ser Leu Lys Tyr Thr Tyr Thr Asn Asn
370 375 380
Leu Glu Thr Tyr Glu Ser Lys Tyr Gly Asn Pro Asn Thr Ser Glu Lys
385 390 395 400
Asn Met Phe Asp Leu Met Asp Arg Asp Ile Tyr Lys Ile Asp Ser Thr
405 410 415
Ala Ala Cys Leu Ala Asn Phe Tyr Thr Tyr Gln Tyr Gly Gly Val Lys
420 425 430
Ala Asp Phe Leu Met Val Lys Arg Asp Ser Ser Ser Thr Thr Ala Tyr
435 440 445
Ser Tyr Ser Lys Thr His Thr Ala Ile Gln Ser Arg Ile Gln Asn Tyr
450 455 460
Asp Ser Glu Asn Glu Leu Pro Gln Glu Thr Thr Asp Gln Pro Asn Tyr
465 470 475 480
Gln Thr Tyr Asn His Arg Leu Ser His Ile Thr Ser Tyr Ser Phe Ser
485 490 495
Asn Ile Gly Asn Ser Asn Gly Gln Phe Gly Asn Leu Pro Val Phe Val
500 505 510
Trp Thr His Arg Ser Ala Asn Ala Asp Asn Phe Ile Phe Leu Asp Lys
515 520 525
Ile Thr Gln Leu Pro Leu Thr Lys Ser Tyr Glu Ile Ser Asp Asn Ala
530 535 540
Thr Ile Val Asn Ser Asp Tyr Thr Gly Gly Ser Leu Leu Lys Val Asn
545 550 555 560
Arg Ser Met Leu Lys Asn Asn Thr Val Asn Ser Ile Arg Phe Ser Leu
565 570 575
Pro Glu Ser Lys Arg Tyr Ile Ala Arg Val Arg Tyr Ala Ser Thr Gly
580 585 590
Asp Gly Tyr Met Asn Met Thr Gly Ile Ser Ser Asp Thr Tyr Phe Asn
595 600 605
Ala Thr Ile Asn Glu Glu Ser Asp Leu Lys Tyr Asp Ser Phe Arg Tyr
610 615 620
Leu Thr Leu Gly Thr Ile Ser Ala Asp Pro Tyr Asn Phe Tyr Ser Leu
625 630 635 640
Tyr Leu Pro Thr Pro Tyr Gly Glu Lys Tyr Thr Ile Tyr Ile Asp Lys
645 650 655
Ile Glu Leu Ile Pro Leu Asp Glu Thr Tyr Glu Val Glu Lys Asn Phe
660 665 670
<210> 230
<211> 192
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 230
Met Ala Ala Ser Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu
1 5 10 15
Ala Gln Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Phe Asp Phe Glu Ala Ile Arg Glu Ala Ala Ala Ala
35 40 45
Val Pro Asp Ser Thr Val Val Lys Met Val Arg Gly Trp Gly Leu Gln
50 55 60
Glu Tyr Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr
85 90 95
Val Glu Arg Glu Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu
100 105 110
Gly Gln Pro Gly Leu Ser Phe Tyr Glu Glu Ser Gly Glu Arg Thr Arg
115 120 125
Phe Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Glu
130 135 140
His Met Tyr Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro
145 150 155 160
Lys Ala Arg Ala Val Ala Leu Glu Leu Thr Asp Ile Val Pro Phe Ala
165 170 175
Val Arg Leu Asn Ala Ile Val Val Arg Gln Ala Leu Asp Leu Val Ala
180 185 190
<210> 231
<211> 963
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 231
Met Val Ser Asn Ser Met Val Pro Tyr Asn Val Leu Arg Asn Met Asn
1 5 10 15
Met Pro Thr Ile Ser Gly Thr Arg Trp Asn Lys Glu Met Phe Ser Ser
20 25 30
Ala Leu Asp Asn Thr Ser Val Leu Leu Asp Leu Ile Glu Lys Gly Ile
35 40 45
Asn Asp Gly Asp Asp Val Leu Gly Val Leu Ser Phe Ile Gly Leu Thr
50 55 60
Ala Leu Glu Ala Ile Pro Val Val Gly Gly Val Leu Ser Ser Leu Val
65 70 75 80
Ser Met Leu Phe Phe Pro Thr Lys Ser Ser Ile Asp Phe Lys Lys Ile
85 90 95
Trp Glu Asn Leu Glu Lys Ala Ile Asn Gln Ile Val Asp Lys Lys Ile
100 105 110
Glu Glu Ala Leu Met Ser Gln Leu Met Gln Glu Ile Ala Gly Leu Ala
115 120 125
Asn Val Leu Lys Glu Tyr Arg Asp Ala Tyr Asp Ser Tyr Asn Gly Lys
130 135 140
Lys Val Phe Asp Ile Pro Asp Arg Leu Thr Pro Glu Glu Tyr Leu Val
145 150 155 160
Gln Val Phe Thr Ser Ala Asn Leu Leu Phe Ile Gln Arg Ile Pro Thr
165 170 175
Phe Gln Asn Pro Lys Tyr Asp Val Leu Phe Leu Pro Phe Phe Val His
180 185 190
Val Ala Glu Met His Ile Leu Leu Ile Arg Asp Ala Ala Ile His Gly
195 200 205
Leu Glu Trp Gly Met Asp Glu Glu Ile His Gln Lys Phe Lys Arg Asn
210 215 220
Leu Lys Glu Ile Ile Asp Glu Tyr Thr Ser Tyr Leu Leu Lys Thr Tyr
225 230 235 240
Asn Lys Gly Leu Lys Glu Val Ser Glu Arg Glu Leu Asn Asp Lys Asp
245 250 255
Phe Pro Thr Thr Asn Asn Lys Asn His Tyr Ile Asn Thr Val Arg Trp
260 265 270
Asn Val Ile Asn Gln Tyr Lys Arg Gly Met Thr Leu Thr Ala Phe Asp
275 280 285
Phe Ala Tyr Lys Trp Lys Tyr Tyr His Glu Val Tyr Gln Asn Asn Ile
290 295 300
Thr Leu Asn Pro Phe Arg Thr Ile Tyr Ser Asp Ile Ala Gly Ser Val
305 310 315 320
Tyr Pro Tyr Ile Lys Thr Thr Asn Glu Ile Asn Asn Thr Ile Lys Asn
325 330 335
Gln Asn Leu Lys Tyr Arg Gly Ile Leu Lys Arg Leu Gln Val Tyr His
340 345 350
Ala Glu Arg Ile Asp Ser Ile Gln Ser Thr Tyr Ile Arg Asn Asn Glu
355 360 365
Ile Ile Asn Ser Thr Lys Ile Gly Gly Val Gly Gly Arg Ile Ser Ser
370 375 380
Leu Val Leu Glu Asp Pro Ile Lys Asn Pro Leu Ile Gln Val Asn Met
385 390 395 400
Trp Ser Glu Leu Val Pro Ile Ser Leu Gly Phe Lys Phe Tyr Asp Glu
405 410 415
Lys Glu Gln Asn Ile Gly Gly Gly Ser Trp Trp Pro Lys Pro His Lys
420 425 430
Phe Gly Ala Tyr His Phe Val Gly Asn Lys Val Ser Ser Ile Ile Gly
435 440 445
Phe Gly Lys Asn Glu Thr Gly Gly Phe Asn Ser Leu Asp Ala Met Ile
450 455 460
Val Gly Phe Lys Arg Asp Asp Tyr Thr Thr Glu Asn Arg Leu Leu Gly
465 470 475 480
Ile Asn Glu Thr Gly Glu Pro Val Thr Lys Val Ile Asp Ala Gly Asn
485 490 495
Phe His Gln Glu Lys Phe Gly Ser Asn Ile Gln Ile Thr Asp Glu Pro
500 505 510
Met Phe Gly Asp Gly Val Leu Gln Phe Thr Asn Tyr Ala Asn Thr Met
515 520 525
Asn Gln Asp Ser Ser Val Thr Tyr Gln Ile Asn Thr Glu Ile Glu Gly
530 535 540
Ile Tyr Gln Leu His Ala Ile Ile Gly Ala Lys Lys Gln Lys Asp Lys
545 550 555 560
Leu Ser Phe Lys Val Ile Leu Asn Thr Lys Gln Gln Lys Lys Ile Ile
565 570 575
Thr Asp Ser Phe Asp Ser His Asp Ile Trp Glu Gly Met Ser Leu Asn
580 585 590
Glu Ser Ile Val Tyr Lys Arg Ile Leu Ile Gly Thr Phe Pro Leu Lys
595 600 605
Arg Gly Met Asn Ser Ile Thr Ile His Asn Asp Val Leu Gln Ser Ser
610 615 620
Ala Asn Ile Lys Thr Trp Asn Leu Gly Ser Leu Glu Leu Thr Met Asp
625 630 635 640
Ser Asp Ser Ile Lys Asp Pro Asp Ile Thr Thr Leu Tyr Asp Lys Asp
645 650 655
Asn Tyr Ala Gly Thr Lys Lys Tyr Ile Phe Glu Asp Thr Ala Arg Leu
660 665 670
Ser Asp Phe Asn Asp Arg Thr Ser Ser Ile Lys Val Gly Ser His Ile
675 680 685
Ala Gly Val Arg Phe Tyr Glu His Tyr His Tyr Ser Gly Asp Phe Ile
690 695 700
Asp Leu Gly Ser Gly Glu Lys Leu Ser Leu Lys Asn His Arg Leu Asn
705 710 715 720
Lys Arg Leu Ser Ser Val Lys Phe Ala Asn Leu Gly Leu Tyr Lys Glu
725 730 735
Asp Asn Tyr Lys Gly Glu Lys Lys Leu Ile Phe Asp Asp Ile Pro Asn
740 745 750
Leu Thr Asp Phe Asn Asp Lys Thr Ser Ser Ile Val Val Ser Pro Asn
755 760 765
Val Ser Gly Thr Val Arg Leu Phe Glu His Ser Tyr Tyr Lys Gly Glu
770 775 780
Tyr Ile Glu Val Ser Gly Gly Gln Lys Leu Ser Leu Lys Gly His Asn
785 790 795 800
Leu Asn Arg Lys Ile Ser Ser Ile Lys Phe Leu Lys Glu His Glu Val
805 810 815
Ile Asn Gly Leu Tyr Glu Ile Ile Thr Ala Ile Asn Asn Lys Ser Val
820 825 830
Val Asp Trp Ser Lys Asp Lys Asn Ser Asn Val His Leu Trp Glu Arg
835 840 845
Arg Asn Gln Asp Asn Gln Lys Trp Tyr Phe Asp Tyr Asp Ser Ser Lys
850 855 860
Gln Ala Phe Gln Ile Lys Ser Lys Ala Asp Lys Asn Ile Val Leu Ala
865 870 875 880
Trp Asn Asp Phe Gly Gly Ser Lys Asn Val Phe Ala Thr Tyr Asn Ser
885 890 895
Lys Lys Asp Glu His Tyr Trp Ile Leu Asp Tyr Leu Gly Glu Gly Asn
900 905 910
Tyr Leu Ile Arg Asn Lys Lys Lys Gln Asn Leu Val Leu Asp Val Asp
915 920 925
Gly His Lys Thr Ala Asn Gly Thr Asn Ile Lys Val Asn Glu Gln His
930 935 940
Ala Leu Asp Asn Pro Ser Ile Glu Ala Gln Lys Phe Lys Leu Asn Tyr
945 950 955 960
Val Ile Lys
<210> 232
<211> 935
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 232
Met Phe Ser Ser Ala Leu Asp Asn Thr Ser Val Leu Leu Asp Leu Ile
1 5 10 15
Glu Lys Gly Ile Asn Asp Gly Asp Asp Val Leu Gly Val Leu Ser Phe
20 25 30
Ile Gly Leu Thr Ala Leu Glu Ala Ile Pro Val Val Gly Gly Val Leu
35 40 45
Ser Ser Leu Val Ser Met Leu Phe Phe Pro Thr Lys Ser Ser Ile Asp
50 55 60
Phe Lys Lys Ile Trp Glu Asn Leu Glu Lys Ala Ile Asn Gln Ile Val
65 70 75 80
Asp Lys Lys Ile Glu Glu Ala Leu Met Ser Gln Leu Met Gln Glu Ile
85 90 95
Ala Gly Leu Ala Asn Val Leu Lys Glu Tyr Arg Asp Ala Tyr Asp Ser
100 105 110
Tyr Asn Gly Lys Lys Val Phe Asp Ile Pro Asp Arg Leu Thr Pro Glu
115 120 125
Glu Tyr Leu Val Gln Val Phe Thr Ser Ala Asn Leu Leu Phe Ile Gln
130 135 140
Arg Ile Pro Thr Phe Gln Asn Pro Lys Tyr Asp Val Leu Phe Leu Pro
145 150 155 160
Phe Phe Val His Val Ala Glu Met His Ile Leu Leu Ile Arg Asp Ala
165 170 175
Ala Ile His Gly Leu Glu Trp Gly Met Asp Glu Glu Ile His Gln Lys
180 185 190
Phe Lys Arg Asn Leu Lys Glu Ile Ile Asp Glu Tyr Thr Ser Tyr Leu
195 200 205
Leu Lys Thr Tyr Asn Lys Gly Leu Lys Glu Val Ser Glu Arg Glu Leu
210 215 220
Asn Asp Lys Asp Phe Pro Thr Thr Asn Asn Lys Asn His Tyr Ile Asn
225 230 235 240
Thr Val Arg Trp Asn Val Ile Asn Gln Tyr Lys Arg Gly Met Thr Leu
245 250 255
Thr Ala Phe Asp Phe Ala Tyr Lys Trp Lys Tyr Tyr His Glu Val Tyr
260 265 270
Gln Asn Asn Ile Thr Leu Asn Pro Phe Arg Thr Ile Tyr Ser Asp Ile
275 280 285
Ala Gly Ser Val Tyr Pro Tyr Ile Lys Thr Thr Asn Glu Ile Asn Asn
290 295 300
Thr Ile Lys Asn Gln Asn Leu Lys Tyr Arg Gly Ile Leu Lys Arg Leu
305 310 315 320
Gln Val Tyr His Ala Glu Arg Ile Asp Ser Ile Gln Ser Thr Tyr Ile
325 330 335
Arg Asn Asn Glu Ile Ile Asn Ser Thr Lys Ile Gly Gly Val Gly Gly
340 345 350
Arg Ile Ser Ser Leu Val Leu Glu Asp Pro Ile Lys Asn Pro Leu Ile
355 360 365
Gln Val Asn Met Trp Ser Glu Leu Val Pro Ile Ser Leu Gly Phe Lys
370 375 380
Phe Tyr Asp Glu Lys Glu Gln Asn Ile Gly Gly Gly Ser Trp Trp Pro
385 390 395 400
Lys Pro His Lys Phe Gly Ala Tyr His Phe Val Gly Asn Lys Val Ser
405 410 415
Ser Ile Ile Gly Phe Gly Lys Asn Glu Thr Gly Gly Phe Asn Ser Leu
420 425 430
Asp Ala Met Ile Val Gly Phe Lys Arg Asp Asp Tyr Thr Thr Glu Asn
435 440 445
Arg Leu Leu Gly Ile Asn Glu Thr Gly Glu Pro Val Thr Lys Val Ile
450 455 460
Asp Ala Gly Asn Phe His Gln Glu Lys Phe Gly Ser Asn Ile Gln Ile
465 470 475 480
Thr Asp Glu Pro Met Phe Gly Asp Gly Val Leu Gln Phe Thr Asn Tyr
485 490 495
Ala Asn Thr Met Asn Gln Asp Ser Ser Val Thr Tyr Gln Ile Asn Thr
500 505 510
Glu Ile Glu Gly Ile Tyr Gln Leu His Ala Ile Ile Gly Ala Lys Lys
515 520 525
Gln Lys Asp Lys Leu Ser Phe Lys Val Ile Leu Asn Thr Lys Gln Gln
530 535 540
Lys Lys Ile Ile Thr Asp Ser Phe Asp Ser His Asp Ile Trp Glu Gly
545 550 555 560
Met Ser Leu Asn Glu Ser Ile Val Tyr Lys Arg Ile Leu Ile Gly Thr
565 570 575
Phe Pro Leu Lys Arg Gly Met Asn Ser Ile Thr Ile His Asn Asp Val
580 585 590
Leu Gln Ser Ser Ala Asn Ile Lys Thr Trp Asn Leu Gly Ser Leu Glu
595 600 605
Leu Thr Met Asp Ser Asp Ser Ile Lys Asp Pro Asp Ile Thr Thr Leu
610 615 620
Tyr Asp Lys Asp Asn Tyr Ala Gly Thr Lys Lys Tyr Ile Phe Glu Asp
625 630 635 640
Thr Ala Arg Leu Ser Asp Phe Asn Asp Arg Thr Ser Ser Ile Lys Val
645 650 655
Gly Ser His Ile Ala Gly Val Arg Phe Tyr Glu His Tyr His Tyr Ser
660 665 670
Gly Asp Phe Ile Asp Leu Gly Ser Gly Glu Lys Leu Ser Leu Lys Asn
675 680 685
His Arg Leu Asn Lys Arg Leu Ser Ser Val Lys Phe Ala Asn Leu Gly
690 695 700
Leu Tyr Lys Glu Asp Asn Tyr Lys Gly Glu Lys Lys Leu Ile Phe Asp
705 710 715 720
Asp Ile Pro Asn Leu Thr Asp Phe Asn Asp Lys Thr Ser Ser Ile Val
725 730 735
Val Ser Pro Asn Val Ser Gly Thr Val Arg Leu Phe Glu His Ser Tyr
740 745 750
Tyr Lys Gly Glu Tyr Ile Glu Val Ser Gly Gly Gln Lys Leu Ser Leu
755 760 765
Lys Gly His Asn Leu Asn Arg Lys Ile Ser Ser Ile Lys Phe Leu Lys
770 775 780
Glu His Glu Val Ile Asn Gly Leu Tyr Glu Ile Ile Thr Ala Ile Asn
785 790 795 800
Asn Lys Ser Val Val Asp Trp Ser Lys Asp Lys Asn Ser Asn Val His
805 810 815
Leu Trp Glu Arg Arg Asn Gln Asp Asn Gln Lys Trp Tyr Phe Asp Tyr
820 825 830
Asp Ser Ser Lys Gln Ala Phe Gln Ile Lys Ser Lys Ala Asp Lys Asn
835 840 845
Ile Val Leu Ala Trp Asn Asp Phe Gly Gly Ser Lys Asn Val Phe Ala
850 855 860
Thr Tyr Asn Ser Lys Lys Asp Glu His Tyr Trp Ile Leu Asp Tyr Leu
865 870 875 880
Gly Glu Gly Asn Tyr Leu Ile Arg Asn Lys Lys Lys Gln Asn Leu Val
885 890 895
Leu Asp Val Asp Gly His Lys Thr Ala Asn Gly Thr Asn Ile Lys Val
900 905 910
Asn Glu Gln His Ala Leu Asp Asn Pro Ser Ile Glu Ala Gln Lys Phe
915 920 925
Lys Leu Asn Tyr Val Ile Lys
930 935
<210> 233
<211> 667
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 233
Met Asn Ser Tyr Gln Asn Lys Asn Lys Tyr Glu Ile Leu Asp Thr Ser
1 5 10 15
Pro Asn Ser Ser Thr Met Ser Thr Arg Tyr Pro Arg Tyr Pro Leu Ala
20 25 30
Lys Asn Pro Gln Ile Ser Met Gln Asn Thr Asn Tyr Lys Asp Trp Ile
35 40 45
Asn Met Cys Ala Ser Arg Asn Leu Glu Asp Arg Ile Phe Pro Thr Ser
50 55 60
Ala Asn Asp Val Ile Thr Asn Ser Ile Asn Ile Ser Ser Tyr Ile Ile
65 70 75 80
Ser Met Leu Gly Met Pro Asn Leu Ser Ser Ile Ile Ala Ile Trp Gly
85 90 95
Ile Leu Phe Asn Ala Phe Trp Pro Val Ser Gly Asn Gln Trp Asp Leu
100 105 110
Tyr Met Asn His Val Gln Ala Leu Ile Lys Arg Glu Leu Gln Asn Phe
115 120 125
Ala Arg Glu Gln Ala Leu Arg Gln Leu Glu Gly Leu Gly Gly Asn Leu
130 135 140
Asp Leu Tyr Lys Glu Ala Leu Ala Glu Trp Glu Gln Asp Arg Asp Asn
145 150 155 160
Pro Thr Thr Lys Glu Arg Val Arg Asp Arg Phe Arg Ile Leu Asp Gly
165 170 175
Phe Phe Thr Gln Tyr Ile Pro Val Phe Arg Ile Gln Gly Tyr Glu Val
180 185 190
Gln Leu Leu Ser Val Tyr Val Lys Val Ala Asn Leu His Leu Leu Leu
195 200 205
Leu Arg Asp Ala Ser Met Phe Gly Ala Asp Trp Gly Met Ser Gln Thr
210 215 220
Asn Ile Asn Asp Asn Tyr Asn Arg Gln Met Lys Leu Thr Ser Leu Tyr
225 230 235 240
Thr Asn His Cys Val Asp Phe Tyr Asn Gln Gly Leu Asn Glu Ala Arg
245 250 255
Ala Leu Ser Asn Ser Asn Trp Asp Ile Phe Asn Asp Tyr Arg Arg Glu
260 265 270
Met Thr Leu Thr Val Leu Asp Ile Val Ala Leu Phe Pro Ala Tyr Asp
275 280 285
Tyr Arg Arg Tyr Pro Ile Thr Thr Lys Val Glu Leu Thr Arg Glu Ile
290 295 300
Tyr Thr Pro Ala Ile Ala Ser Gln Thr Trp Ser Asn His Asn His Leu
305 310 315 320
Ser Pro Asn Glu Asn Phe Gln Phe Tyr Glu Asn Asn Leu Val Arg Pro
325 330 335
Pro Ala Phe Phe Thr Trp Leu Asp Arg Thr Glu Met Phe Ser Arg Tyr
340 345 350
Leu Ser Thr Leu Val Ser Glu Ala Trp Gly Gly His Ile Asn His Phe
355 360 365
His His Thr Gly Glu Leu Pro Ser Ser Ser Arg Ser Gly Phe Ile Gly
370 375 380
Ser Asp Gln Arg Arg Val Ser Tyr Tyr Asp Phe Asn Phe Val Gly Asn
385 390 395 400
Asp Val Phe Arg Ile Tyr Ser Arg Val Met Ser Asn Gln Ile Gly Asn
405 410 415
Tyr Phe Gly Val Gly Gln Gly Asp Phe Phe Leu Val Asn Arg Asp Asn
420 425 430
Ser Asn Thr Lys Thr Ile Ser Phe Thr Thr Arg Ala Thr Asn Ser Asn
435 440 445
Gln Arg Ser Ile Phe Ser Gln Phe Pro Gly Glu Asn Ser Asp Pro Pro
450 455 460
Thr Ser Lys Asp Tyr Ser His Arg Leu Ser Trp Ile Ser Gly Ala Phe
465 470 475 480
Ile Gly Ser Asp Leu Ala Asn Val Leu Ser Tyr Gly Trp Thr His Arg
485 490 495
Ser Val Asp Pro Asn Asn Thr Ile Tyr Pro Asp Arg Ile Thr Gln Val
500 505 510
Pro Ala Val Lys Ala Ser Ser Ala Pro Asp Cys Thr Val Ile Pro Gly
515 520 525
Pro Gly Ser Thr Gly Gly His Leu Val Ser Phe Gly Arg Ser Gly Arg
530 535 540
Leu Asn Met Gln Phe Lys Phe Thr Asn Thr Gln Thr Glu Tyr Arg Val
545 550 555 560
Arg Ile Arg Tyr Ala Ser Thr Ala Ile Asn Thr Leu Tyr Phe Ser Phe
565 570 575
Ser Asp Tyr Asn Arg Asp Ile Ala Leu Asn Ser Thr Gly Ala Leu Ser
580 585 590
Leu Thr Asn Leu Arg Asn Glu Asn Phe Ala Tyr Phe Glu Val Pro Gly
595 600 605
Gly Ile Phe Arg Pro Ala Leu Gly Asn Thr Leu Met Ile Ser Asn Trp
610 615 620
Thr Thr Val Ala Pro Leu Leu Val Ile Asp Lys Ile Glu Phe Ile Pro
625 630 635 640
Ile Asn Ala Thr Thr Ala Arg Tyr Glu Arg Lys Gln Glu Leu Glu Lys
645 650 655
Ala Thr Lys Val Val Asn Ser Leu Phe Ile Asn
660 665
<210> 234
<211> 641
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 234
Met Asn Ser Tyr Gln Asn Lys Asn Lys Tyr Glu Ile Leu Asp Thr Ser
1 5 10 15
Pro Asn Ser Ser Thr Met Ser Thr Arg Tyr Pro Arg Tyr Pro Leu Ala
20 25 30
Lys Asn Pro Gln Ile Ser Met Gln Asn Thr Asn Tyr Lys Asp Trp Ile
35 40 45
Asn Met Cys Ala Ser Arg Asn Leu Glu Asp Arg Ile Phe Pro Thr Ser
50 55 60
Ala Asn Asp Val Ile Thr Asn Ser Ile Asn Ile Ser Ser Tyr Ile Ile
65 70 75 80
Ser Met Leu Gly Met Pro Asn Leu Ser Ser Ile Ile Ala Ile Trp Gly
85 90 95
Ile Leu Phe Asn Ala Phe Trp Pro Val Ser Gly Asn Gln Trp Asp Leu
100 105 110
Tyr Met Asn His Val Gln Ala Leu Ile Lys Arg Glu Leu Gln Asn Phe
115 120 125
Ala Arg Glu Gln Ala Leu Arg Gln Leu Glu Gly Leu Gly Gly Asn Leu
130 135 140
Asp Leu Tyr Lys Glu Ala Leu Ala Glu Trp Glu Gln Asp Arg Asp Asn
145 150 155 160
Pro Thr Thr Lys Glu Arg Val Arg Asp Arg Phe Arg Ile Leu Asp Gly
165 170 175
Phe Phe Thr Gln Tyr Ile Pro Val Phe Arg Ile Gln Gly Tyr Glu Val
180 185 190
Gln Leu Leu Ser Val Tyr Val Lys Val Ala Asn Leu His Leu Leu Leu
195 200 205
Leu Arg Asp Ala Ser Met Phe Gly Ala Asp Trp Gly Met Ser Gln Thr
210 215 220
Asn Ile Asn Asp Asn Tyr Asn Arg Gln Met Lys Leu Thr Ser Leu Tyr
225 230 235 240
Thr Asn His Cys Val Asp Phe Tyr Asn Gln Gly Leu Asn Glu Ala Arg
245 250 255
Ala Leu Ser Asn Ser Asn Trp Asp Ile Phe Asn Asp Tyr Arg Arg Glu
260 265 270
Met Thr Leu Thr Val Leu Asp Ile Val Ala Leu Phe Pro Ala Tyr Asp
275 280 285
Tyr Arg Arg Tyr Pro Ile Thr Thr Lys Val Glu Leu Thr Arg Glu Ile
290 295 300
Tyr Thr Pro Ala Ile Ala Ser Gln Thr Trp Ser Asn His Asn His Leu
305 310 315 320
Ser Pro Asn Glu Asn Phe Gln Phe Tyr Glu Asn Asn Leu Val Arg Pro
325 330 335
Pro Ala Phe Phe Thr Trp Leu Asp Arg Thr Glu Met Phe Ser Arg Tyr
340 345 350
Leu Ser Thr Leu Val Ser Glu Ala Trp Gly Gly His Ile Asn His Phe
355 360 365
His His Thr Gly Glu Leu Pro Ser Ser Ser Arg Ser Gly Phe Ile Gly
370 375 380
Ser Asp Gln Arg Arg Val Ser Tyr Tyr Asp Phe Asn Phe Val Gly Asn
385 390 395 400
Asp Val Phe Arg Ile Tyr Ser Arg Val Met Ser Asn Gln Ile Gly Asn
405 410 415
Tyr Phe Gly Val Gly Gln Gly Asp Phe Phe Leu Val Asn Arg Asp Asn
420 425 430
Ser Asn Thr Lys Thr Ile Ser Phe Thr Thr Arg Ala Thr Asn Ser Asn
435 440 445
Gln Arg Ser Ile Phe Ser Gln Phe Pro Gly Glu Asn Ser Asp Pro Pro
450 455 460
Thr Ser Lys Asp Tyr Ser His Arg Leu Ser Trp Ile Ser Gly Ala Phe
465 470 475 480
Ile Gly Ser Asp Leu Ala Asn Val Leu Ser Tyr Gly Trp Thr His Arg
485 490 495
Ser Val Asp Pro Asn Asn Thr Ile Tyr Pro Asp Arg Ile Thr Gln Val
500 505 510
Pro Ala Val Lys Ala Ser Ser Ala Pro Asp Cys Thr Val Ile Pro Gly
515 520 525
Pro Gly Ser Thr Gly Gly His Leu Val Ser Phe Gly Arg Ser Gly Arg
530 535 540
Leu Asn Met Gln Phe Lys Phe Thr Asn Thr Gln Thr Glu Tyr Arg Val
545 550 555 560
Arg Ile Arg Tyr Ala Ser Thr Ala Ile Asn Thr Leu Tyr Phe Ser Phe
565 570 575
Ser Asp Tyr Asn Arg Asp Ile Ala Leu Asn Ser Thr Gly Ala Leu Ser
580 585 590
Leu Thr Asn Leu Arg Asn Glu Asn Phe Ala Tyr Phe Glu Val Pro Gly
595 600 605
Gly Ile Phe Arg Pro Ala Leu Gly Asn Thr Leu Met Ile Ser Asn Trp
610 615 620
Thr Thr Val Ala Pro Leu Leu Val Ile Asp Lys Ile Glu Phe Ile Pro
625 630 635 640
Ile
<210> 235
<211> 572
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 235
Met Arg Thr Leu Leu Ala Gln Leu Leu Glu Pro Gly Gly Arg Val Gly
1 5 10 15
Leu Glu Leu Gly Gln Leu Gly Ala Arg Asp Asp Thr Leu Leu Glu Leu
20 25 30
Cys Gly Gly Gly Leu Glu Val His Thr Gln Leu Leu Arg Arg Gly Arg
35 40 45
Gly Leu His Gly Leu Gly Val Leu Val Arg Arg His Gly Val Lys Gly
50 55 60
Ile Glu His Ala Gly Glu Leu Arg Pro Cys Arg Leu Pro Val Thr Trp
65 70 75 80
Arg Gly Asp Arg His Gly Arg Phe Pro Arg Gly Tyr Pro His Phe Pro
85 90 95
Leu Leu Leu Pro Ala Arg Leu Arg Ala Gly Gly Asp Pro Arg Glu Val
100 105 110
Gly Ala Asp Leu Glu Arg Ala Pro Ala Arg Arg Ala Pro Asp Ala Ala
115 120 125
Arg Arg Asp Thr Gly Arg Gly Leu Arg Ser Ala Pro Gly Gly Arg Arg
130 135 140
Gly Arg Gly Ser Arg Ala Ala Ala Arg Arg Pro Gly Arg Pro Gln Arg
145 150 155 160
Asp Pro Ala Leu His Gly Asp Asp Gly Arg Arg His Pro Gln Gly Pro
165 170 175
Pro Val Arg Gly Gly Gly Gly Gly Val Gly Arg Gly Pro Arg Arg Gly
180 185 190
Ala Arg Pro Ala Pro Ala Arg Arg His Pro Arg Leu Gly Ala Ala Ala
195 200 205
Gly Pro Pro Gly Asp Gly Ala Pro Arg Asp Asp Gly Arg Arg Asp Arg
210 215 220
Ala Gly Arg Arg Gly Arg Arg Arg Pro Arg Arg Pro Ala Val Ala Gly
225 230 235 240
Pro Ser Ala Pro Pro Gln Gly Pro His Leu Pro Asp Pro Arg Arg Arg
245 250 255
Pro Ala Val Ala Gly Gly Pro Val Val Ala Gly Gly Gly Arg Gly Ala
260 265 270
Arg Ser Gly Gly Gly Val His Arg His Arg Ala Arg Pro His Arg Glu
275 280 285
Gln His Pro Gly Pro Pro Arg Arg Gly Glu Glu Gly Pro Glu Ala Ala
290 295 300
Gln Gly Ala Ala Ala Arg Glu Ala Gly Ala Leu Arg Glu Ala Gly His
305 310 315 320
Arg Leu Pro His Arg Arg Pro Ser Leu Gly Val Pro Gln Gly Arg Arg
325 330 335
Gly Leu Arg Pro Glu Arg Glu Ala Ala Pro Arg Pro Gly Leu Asn Arg
340 345 350
Pro His Gly Gly Arg Pro Val Pro Gly Pro Gly Arg Leu Gly Ile Leu
355 360 365
Thr Pro Pro Asp His Arg Asp His Ala Gly Gly Ala Leu Ala Ala Ser
370 375 380
Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu Ala Gln Ala Arg
385 390 395 400
Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro Ala Thr Val Asn
405 410 415
Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ser Ala Val Pro Asp Ser
420 425 430
Asp Val Val Arg Met Val Arg Gly Trp Gly Leu Gln Glu His Ala Pro
435 440 445
Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val Arg Gln Ala Leu
450 455 460
Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr Val Glu Arg Glu
465 470 475 480
Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu Gly Gln Pro Gly
485 490 495
Leu Ser Phe Tyr Glu Glu Ser Gly Asp Arg Thr Arg Phe Tyr Arg Asp
500 505 510
Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp His Met Tyr Ala
515 520 525
Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro Lys Ala Glu Val
530 535 540
Leu Ala Leu Asp Leu Thr Asp Ile Val Pro Phe Thr Val Arg Leu Asn
545 550 555 560
Ala Ile Val Val Arg Gln Ala Leu Ser Val Ser Val
565 570
<210> 236
<211> 192
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 236
Met Ala Ala Ser Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu
1 5 10 15
Ala Gln Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ser Ala
35 40 45
Val Pro Asp Ser Asp Val Val Arg Met Val Arg Gly Trp Gly Leu Gln
50 55 60
Glu His Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr
85 90 95
Val Glu Arg Glu Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu
100 105 110
Gly Gln Pro Gly Leu Ser Phe Tyr Glu Glu Ser Gly Asp Arg Thr Arg
115 120 125
Phe Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp
130 135 140
His Met Tyr Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro
145 150 155 160
Lys Ala Glu Val Leu Ala Leu Asp Leu Thr Asp Ile Val Pro Phe Thr
165 170 175
Val Arg Leu Asn Ala Ile Val Val Arg Gln Ala Leu Ser Val Ser Val
180 185 190
<210> 237
<211> 392
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 237
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Gly Val Gly Ala
1 5 10 15
Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala
20 25 30
Asn Asn Glu Tyr His Thr Ile His Leu Ala Ala Asn Ser Asn Asn Val
35 40 45
Trp Asp Val Tyr Lys Gly Trp Asp Ser Asp Asn Ala Gly Ile Val Leu
50 55 60
Tyr Asn Gly Ser Arg Gly Asp Asn Glu Gln Phe Ala Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Leu Gly Asn Ala Tyr Ile Ser Thr Asp Gly Val Arg Asp Gly Phe
100 105 110
Ser Phe Asn Asp Gln Gly Leu Arg Gln Thr Asn Trp Thr Gly Ala Pro
115 120 125
Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile
130 135 140
Val Asn Gln Gly Thr Gly Lys Val Ala Ser Trp Ala Trp Asn Gly Ile
145 150 155 160
Ser Gly Pro His Ser Ala Glu Tyr Val Asp Leu Asp Glu Ser Asn Pro
165 170 175
Ser Asp Asn Asn Arg Leu Phe His Ile Pro Ala Ala Arg Ser Thr Phe
180 185 190
Ser Leu Pro Thr Leu Pro Ala Val Gly Thr Arg Pro Asn Ala Pro Asp
195 200 205
Tyr Asn Thr Ser Gly Ala Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn
210 215 220
Ser Val Val Val Gly Ala Ser Leu Ile Pro Ser Ile Met Val Lys Asp
225 230 235 240
Asn Gly Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Ala
245 250 255
Leu Val Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu Ile Ile Pro
260 265 270
Ala Gly Ala Thr Ser Lys Tyr Thr Val Lys Ser Gly Val Ser Glu Ile
275 280 285
Asp Gln Lys Lys Met Ser Asp Thr Leu Ala Met Ser Phe Gly Ser Asp
290 295 300
Leu Gly Leu Lys Phe Gly Glu Ser Ser Leu Ser Ile Lys Ser Ser Val
305 310 315 320
Ser Arg Thr Ile Gln Thr Glu Val Thr Lys Thr Ser Thr Asn Ala Thr
325 330 335
Glu Glu Thr Glu Glu Lys Ser Ile Pro Ser Ile Pro Gly Lys Asn Thr
340 345 350
Gly Phe Thr Ala Tyr Gln Leu Val Thr Lys Tyr Thr Leu Lys Arg Thr
355 360 365
Asp Gly Ser Val Val Thr Asp Pro Ser Ile Val Arg Asp Ser Lys Gln
370 375 380
Thr Ile Ala Arg Thr Ile Ala Asn
385 390
<210> 238
<211> 238
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 238
Met Asn Tyr Pro Ile Leu Asn Asn Thr Asn Ile Asn Ser Val Ile Gln
1 5 10 15
Pro Pro Arg Ile His Arg Pro Gly Arg Ile Ile Thr Leu Thr Val Pro
20 25 30
Ser Arg Asn Glu Ser Asn Met Lys Asn Ile Phe Gln Leu Glu Pro Gln
35 40 45
Tyr Ile Ala Gln Ala Leu Thr Ala Ala Gln Ala Phe Gln Gly Ala Ile
50 55 60
Gly Ser Asn Leu Asn Phe Asp Phe Asp Lys Ala Ala Gln Ile Ala His
65 70 75 80
Asn Leu Pro Asn Arg Tyr Ser Asn Val Ile Gly Leu Glu Arg Pro Val
85 90 95
Ile Gln Gln Thr Leu Gln Val Ser Pro Met Ile Ser Lys Leu Lys Glu
100 105 110
Thr Leu Gly Asn Phe Leu His Phe Ile Asp Ile Ser Asn Ala Gly Phe
115 120 125
Trp Asn Glu Val Glu Ala Ala Ile Thr Tyr Thr Phe Thr Asn Leu Tyr
130 135 140
Thr Gln Ala Asn Ser Ala Trp Ile Phe Trp Ser Asn Ser Thr Ser Thr
145 150 155 160
Ser Thr Ser Tyr Tyr Tyr Asn Ile Leu Phe Ala Ile Arg Asp Ser Ser
165 170 175
Asn Arg Lys Met Ile Val Ile Pro Ile Ala Phe Asp Ile Thr Val Arg
180 185 190
Leu Glu Lys Gln Lys Val Leu Trp Ile Thr Ile Lys Asp Trp Ala Glu
195 200 205
Tyr Arg Val Thr Ile Lys Cys Ile Cys Val His Leu Phe Leu Gln Gly
210 215 220
Thr Pro Asn Gln Ser Ile Val Asp Val Phe Asn Pro Lys Ser
225 230 235
<210> 239
<211> 375
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 239
Met Pro Glu Lys Asn Arg Tyr Tyr Ser Ile His Leu Thr Asn Asn Ile
1 5 10 15
Glu Lys Val Trp Asp Val Tyr Lys Gly Trp Thr Ser Asn Asp Ala Gly
20 25 30
Leu Leu Leu Tyr Lys Ala Asn Gly Gln Asp Asn Gln Gln Phe Val Phe
35 40 45
Phe Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Pro Val Gly Leu Gly Gly Gly Tyr Asn Tyr Ser Asp Gly Val Arg
65 70 75 80
Asp Met Ser Asn Tyr Ser Gly Asn Pro Ala Glu Ile Leu Gly Gln Asn
85 90 95
Asp Trp Thr Gly Ala Asp Thr Asp Lys Trp Tyr Leu Arg Asp Lys Gly
100 105 110
Tyr Gly Asn Tyr Glu Val Val Asn Gln Gly His Gly Lys Val Ala Ser
115 120 125
Tyr Ala Ala Thr Asn Ile Ala Pro Tyr Leu His Tyr Val Asp Leu Asp
130 135 140
Asp Ser Asn Pro Ser Asp Gly Asp Arg Leu Phe Lys Val Ser Lys Ser
145 150 155 160
Pro Trp Val Tyr Gly Asp Ile Pro Gly Leu Asn Trp Asp Tyr Gly Thr
165 170 175
Phe Ser Leu Pro Gln Leu Pro Ala Thr Gly Asn Arg Pro Asp Ala Pro
180 185 190
Asn Tyr Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser
195 200 205
Val Val Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Lys Asp Ser
210 215 220
Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu
225 230 235 240
Ile Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ser Val Ile Pro Ser
245 250 255
Gly Tyr Thr Lys Asn Tyr Ser Phe Lys Thr Gly Met Thr Ser Val Asp
260 265 270
Gln Gln Arg Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe
275 280 285
Gly Leu Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser
290 295 300
Arg Thr Ile Gln Thr Glu Ile Thr Ser Thr Asn Thr Glu Ala Ser Glu
305 310 315 320
Glu Thr Val Thr Ser Thr Val Thr Ser Glu Pro Gly Lys Thr Thr Gly
325 330 335
Phe Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp
340 345 350
Gly Thr Ile Val Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr
355 360 365
Ile Ala Arg Lys Asn Ser Gln
370 375
<210> 240
<211> 192
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 240
Met Ala Ala Ser Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu
1 5 10 15
Ala Gln Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Phe Asp Phe Glu Ala Ile Arg Glu Ala Ala Ala Ala
35 40 45
Val Pro Asp Ser Thr Val Val Arg Met Val Arg Gly Trp Gly Leu Gln
50 55 60
Glu Tyr Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr
85 90 95
Val Glu Arg Glu Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu
100 105 110
Gly Arg Pro Gly Leu Ser Phe Tyr Glu Glu Ser Ala Glu Arg Thr Arg
115 120 125
Phe Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Glu
130 135 140
His Met Tyr Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro
145 150 155 160
Lys Ala Glu Thr Val Ala Leu Glu Leu Thr Asp Val Val Pro Phe Ala
165 170 175
Val Arg Leu Asn Ala Ile Val Val Arg Gln Pro Leu Gly Leu Ala Ala
180 185 190
<210> 241
<211> 296
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 241
Met Ala Ile Ala Asp Leu Asp Val Tyr Leu Ile Ala Leu Ile Lys Lys
1 5 10 15
Thr Tyr Pro Glu Tyr Ala Ser Tyr Glu Ala Thr Ser Asp Thr Ser Lys
20 25 30
Leu Ser Ile Lys Asn Thr Glu Ser Tyr Gly Phe Glu Ile Lys Glu Asn
35 40 45
Lys Pro Gln Gly Thr Met Phe Met Gly Glu Ser Val Leu Lys Asn Asp
50 55 60
Thr Asn Glu Thr Gln Thr Ile His Ser Asp Ala Phe Thr Lys Thr Ile
65 70 75 80
Thr Asp Ser Val Thr Leu Ser Val Thr Asn Gly Ile Thr Ala Gly Val
85 90 95
Ser Ile Ser Ile Ser Gly Lys Ile Phe Gly Ile Gly Ala Glu Ser Ser
100 105 110
Met Ser Phe Glu Val Ser Thr Ser Thr Thr Asn Glu Gln Thr Ser Glu
115 120 125
Glu Ser Val Ala Tyr Thr Val Pro Ser Gln Thr Val Val Val Pro Ala
130 135 140
Lys Thr Thr Tyr Tyr Val Tyr Thr Ser Leu Gln Arg Asn Gln Leu Glu
145 150 155 160
Gly Thr Val Arg Leu Lys Ala Asp Leu Ser Glu Arg Phe Ser Val Arg
165 170 175
Leu Lys Tyr Gly Ser Gly Gln Ala Glu Ile Gly Gly Asp Leu Tyr Tyr
180 185 190
Phe Ile Asn Glu Phe His Ser Ala Leu Pro Leu Pro Ser Gly Ile Ser
195 200 205
Leu Asn His Lys Asp Lys Ser Ile His Phe Glu Gly Val Ala Glu Tyr
210 215 220
Ile Tyr Gly Thr Gly Thr Lys Phe Tyr Val Thr Ile Ser Asp Thr Pro
225 230 235 240
Ser Ser Gln Gly Ile Gln Glu His Lys Ser Phe Asp Ala Lys Thr Gly
245 250 255
Leu Gly Thr Tyr Glu Leu Arg Leu Asn Gly Lys Asn Phe Asp Phe Asp
260 265 270
Leu Asn Asp Leu Lys Glu Tyr Met Asp Ser Lys Glu Phe Glu Lys Leu
275 280 285
Lys Glu Gln Glu Asn Glu Ile Val
290 295
<210> 242
<211> 349
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 242
Met Ala Thr Pro Glu Lys Asn Lys Tyr Tyr Asn Ile Asn Leu Lys Ala
1 5 10 15
Asn Thr Trp Leu Lys Trp Asn Val Glu Gly Ala Ser Thr Asn Asn Gly
20 25 30
Pro Thr Ile Arg Leu Trp Gly Gly Ser Asn Asn Glu Asn Glu Lys Phe
35 40 45
Thr Phe Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn
50 55 60
Ser Gly Lys Pro Val Thr Val Pro Ser Gly Ser Ser Ile Leu Ser Gln
65 70 75 80
Asn Asn Trp Thr Gly Ser Ser Asn Glu Gln Trp Tyr Leu Arg Asn Gln
85 90 95
Gly Asn Asn Tyr Tyr Glu Ile Val His Gln Gly Thr Gly Lys Val Ala
100 105 110
Ser Tyr Ala Arg Asn Gly Asn Ala Glu Tyr Val Asp Leu Asp Glu Ser
115 120 125
Asn Pro Tyr Asp Pro Asp Arg Val Phe Gln Ile Ser Asp Ala Gly Ala
130 135 140
Ser Val Gln Leu Pro Thr Leu Pro Thr Ile Gly Ser Arg Pro Asn Ala
145 150 155 160
Pro Glu Tyr Asn Pro Thr Gly Pro Ile Asp Gln Gln Leu Pro Gln Asn
165 170 175
Ser Gly Thr Val Val Val Gly Ala Thr Ser Ile Pro Cys Ile Met Val
180 185 190
Asn Asp Asn Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr
195 200 205
Tyr Val Leu Val Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu Ile
210 215 220
Ile Pro Ala Gly Gly Ser Ser Lys Tyr Thr Val Lys Thr Gly Val Ser
225 230 235 240
Thr Glu Asp Gln Gln Arg Met Thr Asp Thr Leu Ser Met Ser Phe Gly
245 250 255
Ala Asp Leu Gly Phe Lys Phe Lys Ala Leu Ser Ala Ser Leu Lys Tyr
260 265 270
Gly Ile Ser Lys Thr Leu Gln Thr Glu Ile Ser Thr Thr Ser Thr Glu
275 280 285
Ser Thr Glu Ser Thr Glu Ser Thr Glu Glu Lys Thr Ile Thr Ser Leu
290 295 300
Pro Gly Lys Asn Thr Gly Phe Thr Val Tyr Gln Leu Val Thr Lys Tyr
305 310 315 320
Thr Leu Lys Arg Thr Asp Asn Ser Val Val Ser Ser Ser Trp Thr Val
325 330 335
Arg Asp Pro Asn Gln Thr Leu Val Arg Thr Ile Pro Asn
340 345
<210> 243
<211> 553
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 243
Met Asn Lys Gln Asn Thr Gln Val Ala Glu Leu Pro Val Ile Asp Glu
1 5 10 15
Phe Asn Gly Ile Ile Gly Phe Ala Gln Asn Ile Gly Gly Ile Met Asn
20 25 30
Met Ile Ala Gly Thr Pro Ser Val Asp Asp Met Thr Leu Asp Gln Leu
35 40 45
Val Thr Asn Tyr Asp Ala Leu Val Asp Ile Gln Thr Ser Ile Ala Gly
50 55 60
Ile Asn Lys Glu Leu Thr Asp Val Ile Ala Asn Gly Ser Glu Asp Thr
65 70 75 80
Glu Leu Ile Asn Gln Leu Ile Asn Ile Asn Thr Ala Gln Ser Ala Glu
85 90 95
Leu Asp Glu Ile Asn Gly Glu Ile Gln Asp Ile Asp Asn Met Leu Asn
100 105 110
Ala Tyr Leu Pro Glu Ile Ile Asn Met Leu Glu Gly Ile Ser Gln Gln
115 120 125
Leu Val Gly Ile Ser Gln Gln Ile Asn Gln Met Met Lys Met Leu Asn
130 135 140
Ala Ile Ser Lys Gln Leu Asn Lys Ile Ala Ala Asn Val Ala Leu Asn
145 150 155 160
Ser Thr Ile Ala Glu Ile Glu Pro His Tyr Gln Lys Ile Lys Tyr Val
165 170 175
Ile Thr Gln Phe Asn Glu Leu Asn Asp Glu Leu Val Thr Ala Tyr Ser
180 185 190
Ser Gln Ile Asn Asn Asn Lys Leu His Leu Gly Thr Asp Asp Ile Asp
195 200 205
Thr Gly Ala Leu Ala Ala Val Ile Ala Trp Ala Lys Met Val Val Ala
210 215 220
Ile Asp Leu Asn Asp Leu Arg Leu Ser Leu Asp Ala Met Asn Asp Ala
225 230 235 240
Ile Leu Gly Asp Asn Leu Tyr Gly Asn Ser Ala Leu Val Ser Thr Ala
245 250 255
Asp Phe Tyr Thr Ala Asn Thr Thr Tyr Ala Gln Ser Ile Tyr Thr Ser
260 265 270
Met Tyr Ser Tyr Leu Ser Thr Leu Cys Gly Leu Gln Thr Asn Ala Tyr
275 280 285
Met Leu Val Asp Thr Ala Arg Lys Ile Leu Ser Leu Pro Glu Glu Asp
290 295 300
Asn Gln Asp Leu Met Asn Ser Arg Ile Ser Gln Gln Lys Glu Leu Phe
305 310 315 320
Thr Ser Leu Ser Ser Gly Phe Ser Asn Val Phe Tyr Ser Thr Glu Ser
325 330 335
Ile Asp Met Asp Pro Gly Ala Ser Ala Pro Met Asn Gln Ser Tyr Glu
340 345 350
Ile Asp Leu Val Pro Pro Ala Gly Asn Ala Leu Leu Gly Phe Asn Met
355 360 365
Thr Ser Glu Pro Asp Ser Asn Gly Asp Val Gln Asn Gly Lys Leu His
370 375 380
Val Glu Tyr Tyr Tyr Gly Lys Leu Leu Thr Asn Tyr Gly Val Asp Val
385 390 395 400
Ala Ser Ile Asn Val Ala Thr Ala Glu Phe Ser Met Ala Asn Tyr Leu
405 410 415
Pro Ser Ser Gly Ile Tyr Asn Val Pro Asp Gly Gly Leu Pro Thr Ile
420 425 430
Asp Ser Asn Thr Ile Val Val Gly Phe Lys Leu Lys Ile Thr Thr Asp
435 440 445
Asn Met Gly Gln Ala Phe Met Val Pro Ser Val Val Thr Ala Pro Tyr
450 455 460
Asp Ser Thr Lys Asn Gly Val Gly Thr Thr Thr Lys Asp Val Val Ile
465 470 475 480
Pro Gln Ser Asp Asn Asn Ser Ile Thr Asn Gln Pro Met Gly Ile Val
485 490 495
Ser Asp Gln Phe Leu Ser Pro Ile Leu Asn Met Thr Met Gly Lys Asp
500 505 510
Glu Asn Gly Gln Tyr Cys Phe Met Val Glu Ser Ala Leu Lys Asn Tyr
515 520 525
Val Asn Ala Thr Asp Met Lys Asp Ala Asp Thr Asp Leu Ala Val Ser
530 535 540
Pro Tyr Tyr Ser Thr Asp Thr Ala Glu
545 550
<210> 244
<211> 724
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 244
Met Asn Pro Asp Gln Asn Lys Asn Glu Tyr Glu Ile Val Asn Thr Ser
1 5 10 15
Gln Asn Tyr Asp Asn Val Ala Asn Arg Tyr Pro Tyr Ala Asn Asp Pro
20 25 30
Asn Ile Lys Met Gln Asn Thr Asn Tyr Arg Asp Trp Met Asn Phe Cys
35 40 45
Thr Gly Asn Arg Ile Glu Asn Ile Ser Pro Thr Ser Ala Arg Asp Val
50 55 60
Ile Val Thr Ser Thr Asp Ile Ile Gly Arg Ile Leu Gly Met Leu Gly
65 70 75 80
Ile Pro Gly Ala Ser Glu Ile Leu Glu Leu Trp Asn Ile Leu Phe Asn
85 90 95
Ala Leu Trp Pro Ala Pro Asp Ala Gln Trp Val Ala Tyr Ile Asn His
100 105 110
Thr Gln Ala Leu Ile Gln Arg Glu Leu Gln Leu Phe Ala Arg Glu Gln
115 120 125
Ala Leu Arg Gln Leu Glu Gly Leu Gly Asn Asn Leu Asn Leu Tyr Arg
130 135 140
Glu Ala Phe Arg Glu Trp Glu Gln Asp Arg Asn Asn Pro Ser Thr Arg
145 150 155 160
Asn Arg Val Ile Asp Arg Phe Arg Ile Met Asp Ala Phe Leu Thr Gln
165 170 175
Tyr Ile Pro Val Phe Arg Ile Asn Asn Tyr Gln Val Gln Leu Leu Ser
180 185 190
Val Tyr Ala Gln Ala Ala Asn Ile His Leu Leu Leu Leu Arg Asp Ala
195 200 205
Thr Val Phe Gly Ser Asp Trp Gly Leu Thr Ser Thr Asn Ile Asn Asp
210 215 220
Ile Tyr Asn Arg Gln Met Asn Leu Ile Ala Glu Tyr Thr Asn His Cys
225 230 235 240
Thr Thr Trp Tyr Asn Asn Gly Leu Asn Glu Ala Arg Asn Leu Ser Arg
245 250 255
Ser Ser Trp Asp Thr Phe Asn Asn Tyr Arg Arg Asp Met Thr Ile Met
260 265 270
Val Leu Asp Leu Ile Ala Leu Phe Pro Thr Tyr Asp Gln Arg Arg Tyr
275 280 285
Pro Ile Thr Thr Lys Leu Glu Leu Thr Arg Glu Ile Tyr Thr Pro Ala
290 295 300
Ile Ala Ser Gln Asn Trp Ser Asn Phe Asn His Leu Ser Pro Asn Ile
305 310 315 320
Asp Phe Ala Phe Tyr Glu Arg Asn Leu Ile Arg Pro Pro Ser Leu Phe
325 330 335
Thr Trp Leu Asp Arg Thr Glu Met Phe Ser Arg Asn Leu Ser Val Glu
340 345 350
Val Ser Glu Val Trp Gly Gly His Ile Asn Tyr Phe His His Thr Arg
355 360 365
Ser Leu Ala Ala Ser Leu Arg Ser Asn Phe Ile Gly Ser Asp Gln Arg
370 375 380
Arg Val Ser Gln Tyr Asn Phe Asn Phe Val Gly Asp Asp Ile Phe Arg
385 390 395 400
Val Asp Ser Leu Val Gly Ser Asn Thr Val Gly Asn Phe Phe Gly Val
405 410 415
Ile Gln Gly Asn Phe His Leu Val Asn Arg Ser Ser Ser Ser Thr Arg
420 425 430
Ala Ile Pro Phe Thr Thr Arg Ser Thr Asn Pro Ser Arg Arg Ser Ile
435 440 445
Val Ser Glu Leu Pro Gly Glu Asn Ser Asn Ser Pro Asn Phe Arg Asn
450 455 460
Tyr Thr His Arg Leu Ser Trp Ile Ser Gly Ala Phe Ile Gly Ser Asp
465 470 475 480
Ile Ala Asn Val Leu Ala Tyr Gly Trp Thr His Arg Ser Val Asp Pro
485 490 495
Asn Asn Thr Ile Leu Pro Asn Arg Ile Thr Gln Ile Pro Ala Val Lys
500 505 510
Ala Thr Thr Ile Ser Asn Ala Thr Val Val Ser Gly Pro Gly Ser Thr
515 520 525
Gly Gly Asn Leu Val Arg Leu His Asn Gln Gly Arg Phe Gln Ile Gln
530 535 540
Met Asn Phe Pro Thr Pro Gln Thr Asn Tyr Arg Ile Arg Ile Arg Tyr
545 550 555 560
Ala Ser Ile Gly Ser Arg Val Leu His Val Gln Phe Ser Gly Ala Asn
565 570 575
His Gly Pro Thr Ile Ala Pro Thr Thr Val Ser Leu Asp Asn Leu Arg
580 585 590
Tyr Glu Asn Phe Ala Tyr Ile Asp Val Ile Gly Thr Phe Ile Pro Ala
595 600 605
Leu Gly Asn Tyr Val Thr Ile Val Asn Pro Asn Pro Gly Asp Val Val
610 615 620
Ile Asp Lys Ile Glu Phe Ile Pro Ile Gly Thr Phe Ala Asn Gln Ser
625 630 635 640
Leu Gly Glu Thr Glu Val His Asn Asn Asn Tyr Asn Gln Ser Tyr Asn
645 650 655
Asn Tyr Asp Gln Asn Met Asp Asn Met Tyr Pro Pro Ser Tyr Asn Asn
660 665 670
Val Tyr Glu Gln Asn Ser His Asp Ser Tyr Asp Ser Gly Tyr Asn Asn
675 680 685
Thr Tyr Glu Pro Asn Tyr Asp Cys Asn Cys Asn Gln Lys Tyr Asn Asn
690 695 700
Asn Tyr Asn Gln Asn Ser Gly Cys Thr Cys Asn Gln Gly Tyr Asn Ser
705 710 715 720
Asn Tyr Pro Lys
<210> 245
<211> 633
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 245
Met Asn Pro Asp Gln Asn Lys Asn Glu Tyr Glu Ile Val Asn Thr Ser
1 5 10 15
Gln Asn Tyr Asp Asn Val Ala Asn Arg Tyr Pro Tyr Ala Asn Asp Pro
20 25 30
Asn Ile Lys Met Gln Asn Thr Asn Tyr Arg Asp Trp Met Asn Phe Cys
35 40 45
Thr Gly Asn Arg Ile Glu Asn Ile Ser Pro Thr Ser Ala Arg Asp Val
50 55 60
Ile Val Thr Ser Thr Asp Ile Ile Gly Arg Ile Leu Gly Met Leu Gly
65 70 75 80
Ile Pro Gly Ala Ser Glu Ile Leu Glu Leu Trp Asn Ile Leu Phe Asn
85 90 95
Ala Leu Trp Pro Ala Pro Asp Ala Gln Trp Val Ala Tyr Ile Asn His
100 105 110
Thr Gln Ala Leu Ile Gln Arg Glu Leu Gln Leu Phe Ala Arg Glu Gln
115 120 125
Ala Leu Arg Gln Leu Glu Gly Leu Gly Asn Asn Leu Asn Leu Tyr Arg
130 135 140
Glu Ala Phe Arg Glu Trp Glu Gln Asp Arg Asn Asn Pro Ser Thr Arg
145 150 155 160
Asn Arg Val Ile Asp Arg Phe Arg Ile Met Asp Ala Phe Leu Thr Gln
165 170 175
Tyr Ile Pro Val Phe Arg Ile Asn Asn Tyr Gln Val Gln Leu Leu Ser
180 185 190
Val Tyr Ala Gln Ala Ala Asn Ile His Leu Leu Leu Leu Arg Asp Ala
195 200 205
Thr Val Phe Gly Ser Asp Trp Gly Leu Thr Ser Thr Asn Ile Asn Asp
210 215 220
Ile Tyr Asn Arg Gln Met Asn Leu Ile Ala Glu Tyr Thr Asn His Cys
225 230 235 240
Thr Thr Trp Tyr Asn Asn Gly Leu Asn Glu Ala Arg Asn Leu Ser Arg
245 250 255
Ser Ser Trp Asp Thr Phe Asn Asn Tyr Arg Arg Asp Met Thr Ile Met
260 265 270
Val Leu Asp Leu Ile Ala Leu Phe Pro Thr Tyr Asp Gln Arg Arg Tyr
275 280 285
Pro Ile Thr Thr Lys Leu Glu Leu Thr Arg Glu Ile Tyr Thr Pro Ala
290 295 300
Ile Ala Ser Gln Asn Trp Ser Asn Phe Asn His Leu Ser Pro Asn Ile
305 310 315 320
Asp Phe Ala Phe Tyr Glu Arg Asn Leu Ile Arg Pro Pro Ser Leu Phe
325 330 335
Thr Trp Leu Asp Arg Thr Glu Met Phe Ser Arg Asn Leu Ser Val Glu
340 345 350
Val Ser Glu Val Trp Gly Gly His Ile Asn Tyr Phe His His Thr Arg
355 360 365
Ser Leu Ala Ala Ser Leu Arg Ser Asn Phe Ile Gly Ser Asp Gln Arg
370 375 380
Arg Val Ser Gln Tyr Asn Phe Asn Phe Val Gly Asp Asp Ile Phe Arg
385 390 395 400
Val Asp Ser Leu Val Gly Ser Asn Thr Val Gly Asn Phe Phe Gly Val
405 410 415
Ile Gln Gly Asn Phe His Leu Val Asn Arg Ser Ser Ser Ser Thr Arg
420 425 430
Ala Ile Pro Phe Thr Thr Arg Ser Thr Asn Pro Ser Arg Arg Ser Ile
435 440 445
Val Ser Glu Leu Pro Gly Glu Asn Ser Asn Ser Pro Asn Phe Arg Asn
450 455 460
Tyr Thr His Arg Leu Ser Trp Ile Ser Gly Ala Phe Ile Gly Ser Asp
465 470 475 480
Ile Ala Asn Val Leu Ala Tyr Gly Trp Thr His Arg Ser Val Asp Pro
485 490 495
Asn Asn Thr Ile Leu Pro Asn Arg Ile Thr Gln Ile Pro Ala Val Lys
500 505 510
Ala Thr Thr Ile Ser Asn Ala Thr Val Val Ser Gly Pro Gly Ser Thr
515 520 525
Gly Gly Asn Leu Val Arg Leu His Asn Gln Gly Arg Phe Gln Ile Gln
530 535 540
Met Asn Phe Pro Thr Pro Gln Thr Asn Tyr Arg Ile Arg Ile Arg Tyr
545 550 555 560
Ala Ser Ile Gly Ser Arg Val Leu His Val Gln Phe Ser Gly Ala Asn
565 570 575
His Gly Pro Thr Ile Ala Pro Thr Thr Val Ser Leu Asp Asn Leu Arg
580 585 590
Tyr Glu Asn Phe Ala Tyr Ile Asp Val Ile Gly Thr Phe Ile Pro Ala
595 600 605
Leu Gly Asn Tyr Val Thr Ile Val Asn Pro Asn Pro Gly Asp Val Val
610 615 620
Ile Asp Lys Ile Glu Phe Ile Pro Ile
625 630
<210> 246
<211> 393
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 246
Met Gln Met Val Thr Lys Ala Met Thr Thr Lys Leu Gly Ala Leu Pro
1 5 10 15
Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Asp Ser Ile
20 25 30
Thr Gly Val Met Ser Thr Ile Phe Gly Ile Asp Thr Gly Asp Ser Ser
35 40 45
Ile Asp Asp Val Leu Asn Asn Gln Lys Leu Leu Gln Glu Met Gln Ala
50 55 60
Gln Met Gln Gln Met Gln Asp Ser Ile Thr Asp Met Ala Asn Gln Gln
65 70 75 80
Met Ile Ser Glu Asp Ile Glu Lys Gln Ile Leu Ala Leu Thr Thr Glu
85 90 95
Leu Ser Ala Ser Ile Thr Thr Glu Phe Asn Lys Ile Glu Gly Ile Leu
100 105 110
Asn Thr Tyr Leu Pro Ala Ile Ser Lys Met Leu Gly Glu Val Tyr Glu
115 120 125
Gln Thr Ser Glu Ile Asn Glu Lys Val Asp Lys Leu Leu Ala Met Met
130 135 140
Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val Val Leu
145 150 155 160
Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu Asp Tyr
165 170 175
Val Asn Lys Lys Phe Leu Ser Leu Thr Lys Ser Phe Leu Gln Asn Glu
180 185 190
Asp Leu Ser Ile Asp Ser Met Gln Glu Met Leu Glu Trp Ala Lys Ser
195 200 205
Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp Thr Leu
210 215 220
His Ser Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala Leu Lys
225 230 235 240
Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asp Gln Tyr Gly Asp Phe
245 250 255
Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu Ala Thr
260 265 270
Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys Ile Leu
275 280 285
Gly Leu Ser Glu Phe Asp Tyr Leu Val Thr Met Glu Asp Arg Ile Glu
290 295 300
Lys Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Met Asn Tyr Ser
305 310 315 320
Asn Ala Leu Glu Ile Asn Gly Ile Tyr Pro Ile Pro Ser Arg Trp Gly
325 330 335
Glu Cys Lys Ser Leu Asp Leu Gln Ala Glu Ser Gly Tyr Ala Ile Ile
340 345 350
Gly Leu Glu Phe Phe Met Glu Asn Gly Ile Tyr Lys Ala Lys Ala Tyr
355 360 365
Gln Gly Glu Ile Gly Lys Asn Phe Ser Val Ser Ala Asp Thr Val Ser
370 375 380
Glu Ile Ile Ser Asp Asp Phe Lys Phe
385 390
<210> 247
<211> 278
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 247
Met Thr Thr Lys Asn Leu Ser Glu Leu Glu Met Glu Ile Ser Asn Asn
1 5 10 15
Gln Gln Gln Pro Arg Arg Leu Ile Val Pro Ala Phe Arg His Ile Val
20 25 30
Leu Lys Val Pro Ala Ser Glu Glu Ser Asn Tyr Lys Glu Ile Phe Gln
35 40 45
Val Glu Pro Arg Tyr Ile Ala Gln Ala Leu Arg Leu Ala Asn Ala Phe
50 55 60
Gln Gly Ala Leu Asp Pro Thr Asp Leu Asn Phe Asn Phe Glu Lys Ala
65 70 75 80
Leu Gln Ile Ala Gln His Ile Pro Asn Ser Ala Ile Val Lys Thr Leu
85 90 95
Asn Gln Asn Val Val Gln Gln Thr Val Glu Ile Ser Ala Met Ile Ser
100 105 110
Gln Leu Lys Gln Ile Ile Arg Asp Val Leu Gly Leu Val Ile Ser Ser
115 120 125
Pro Asn Phe Trp Asn Ser Val Glu Ala Ala Val Thr Asn Thr Phe Thr
130 135 140
Asn Leu Asn Thr Gln Glu Asp Gly Ala Trp Ile Phe Trp Lys Ser Ser
145 150 155 160
Ser Ser Thr Asn Thr Ser Tyr Tyr Tyr Asn Ile Leu Phe Ser Ile Gln
165 170 175
Asn Ala Glu Thr Gly Ser Val Met Ala Val Leu Pro Ile Ala Phe Glu
180 185 190
Ile Ser Val Asn Leu Glu Lys Gln Lys Val Leu Phe Leu Thr Ile Arg
195 200 205
Asp Ser Ala Arg Tyr Glu Val Lys Leu Lys Ala Leu Thr Leu Val Gln
210 215 220
Ala Leu Asp Ser Phe Asn Ala Pro Ile Glu Asp Val Phe Asn Val His
225 230 235 240
Asn Tyr Gly Pro Tyr Pro Pro Asn Asn Ser Ser Thr Asn Asn Tyr Ile
245 250 255
Leu Gln Asn Leu Lys Phe Asn Gln Ser Ser Thr Asn Asn Leu Ile Val
260 265 270
Gln Asp Thr Ile Asp Asn
275
<210> 248
<211> 239
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 248
Met Asn Tyr Pro Ile Leu Asn Asn Thr Asn Ile Ser Ser Ser Leu Gln
1 5 10 15
Gln Pro Arg Ile His Arg Pro Ser Arg Thr Ile Ile Leu Thr Val Pro
20 25 30
Ser Arg Asp Glu Ser Asn Ile Arg Asn Ile Phe Gln Val Glu Pro Arg
35 40 45
Tyr Ile Ala Arg Ala Leu Thr Ala Ala Gln Ala Phe Gln Gly Ala Ile
50 55 60
Gly Ser Asp Leu Asn Phe Asn Tyr Asp Lys Ala Ile Gln Ile Ala Asn
65 70 75 80
Asn Ile Pro Tyr Arg Asp His Ser Asn Ile Ser Trp His Met Asn Arg
85 90 95
Thr Val Ile Gln Glu Thr Leu Gln Val Ser Pro Met Ile Ser Lys Ile
100 105 110
Lys Asp Ile Leu Gly Gly Phe Leu Tyr Phe Ile Asn Ile Ser Asn Ala
115 120 125
Gly Phe Trp Asn Glu Val Glu Ala Ala Ile Ile Asn Thr Phe Thr Asn
130 135 140
Leu Tyr Thr Gln Thr Asn Ser Ala Trp Ile Phe Trp Ser Ser Ser Thr
145 150 155 160
Ser Thr Ser Thr Ser Tyr Tyr Tyr Asn Ile Leu Phe Ala Ile Pro Arg
165 170 175
Thr Ser Lys Ser Met Val Val Ile Pro Ile Ala Phe Asp Ile Thr Val
180 185 190
Arg Leu Glu Lys Gln Lys Val Leu Phe Ile Thr Ile Lys Asp Arg Ala
195 200 205
Glu Phe Arg Val Thr Ile Lys Ala Ile Tyr Val His Val Leu Leu His
210 215 220
Ser Leu Ser Asn Gln Ser Ile Val Asp Val Phe Asn Pro Lys Ser
225 230 235
<210> 249
<211> 572
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 249
Met Arg Thr Leu Leu Ala Gln Leu Leu Glu Pro Gly Gly Arg Val Gly
1 5 10 15
Leu Glu Leu Gly Gln Leu Gly Ala Arg Asp Asp Thr Leu Leu Glu Leu
20 25 30
Cys Gly Gly Gly Leu Glu Val His Thr Gln Leu Leu Arg Arg Gly Arg
35 40 45
Gly Leu His Gly Leu Gly Val Leu Val Arg Arg His Gly Val Lys Gly
50 55 60
Ile Glu His Ala Gly Glu Leu Arg Pro Cys Arg Leu Pro Val Thr Trp
65 70 75 80
Arg Gly Asp Arg Leu Gly Arg Phe Pro Arg Gly Tyr Pro His Phe Pro
85 90 95
Leu Leu Leu Pro Ala Arg Leu Arg Ala Gly Gly Asp Pro Arg Glu Val
100 105 110
Gly Ala Asp Leu Glu Arg Ala Pro Ala Arg Arg Ala Pro Asp Ala Ala
115 120 125
Arg Arg Asp Thr Gly Arg Gly Leu Arg Arg Ala Pro Gly Gly Arg Arg
130 135 140
Gly Arg Gly Ser Arg Ala Ala Ala Arg Arg Pro Gly Arg Pro Gln Arg
145 150 155 160
Asp Pro Ala Leu His Gly Asp Asp Arg Arg Arg His Pro Gln Gly Pro
165 170 175
Pro Val Arg Gly Gly Gly Gly Gly Val Gly Arg Gly Pro Arg Arg Gly
180 185 190
Ala Arg Pro Ala Pro Ala Arg Arg His Pro Arg Leu Gly Ala Ala Ala
195 200 205
Gly Pro Pro Gly Asp Gly Ala Ala Arg Asp Asp Gly Arg Arg Asp Arg
210 215 220
Ala Gly Arg Arg Gly Arg Arg Arg Pro Arg Arg Pro Pro Val Ala Gly
225 230 235 240
Pro Ser Ala Pro Pro Gln Gly Pro His Leu Pro Asp Pro Arg Arg Arg
245 250 255
Pro Ala Val Ala Gly Gly Pro Val Val Ala Gly Arg Gly Arg Gly Ala
260 265 270
Arg Pro Gly Gly Gly Val His Arg His Arg Ala Arg Pro His Arg Glu
275 280 285
Gln His Pro Gly Pro Pro Gly Arg Gly Glu Glu Gly Pro Glu Ala Ala
290 295 300
Gln Gly Ala Ala Ala Arg Glu Ala Gly Ala Leu Arg Glu Ala Gly Arg
305 310 315 320
Arg Leu Pro His Leu Arg Pro Ser Pro Gly Val Pro Gln Gly Arg Arg
325 330 335
Arg Leu Arg Pro Glu Arg Glu Ala Ala Pro Arg Pro Gly Leu Ser Arg
340 345 350
Pro His Gly Gly Arg Pro Ala Pro Gly Arg Arg Arg Leu Gly Ile Leu
355 360 365
Thr Pro Pro Asp His Arg Asp His Ala Gly Gly Ala Leu Ala Ala Ser
370 375 380
Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu Ala Gln Ala Arg
385 390 395 400
Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro Ala Thr Val Asn
405 410 415
Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ser Ala Val Pro Asp Ser
420 425 430
Asp Val Val Arg Met Val Arg Gly Trp Gly Leu Gln Glu His Ala Pro
435 440 445
Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val Arg Gln Ala Leu
450 455 460
Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr Val Glu Arg Glu
465 470 475 480
Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu Gly Gln Pro Gly
485 490 495
Leu Ser Phe Tyr Glu Glu Ser Gly Asp Arg Thr Arg Phe Tyr Arg Asp
500 505 510
Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp His Met Tyr Ala
515 520 525
Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro Lys Ala Glu Val
530 535 540
Leu Ala Leu Asp Leu Thr Asp Ile Val Pro Phe Thr Val Arg Leu Asn
545 550 555 560
Ala Ile Val Val Arg Gln Ala Leu Ser Val Ser Val
565 570
<210> 250
<211> 192
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 250
Met Ala Ala Ser Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu
1 5 10 15
Ala Gln Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ser Ala
35 40 45
Val Pro Asp Ser Asp Val Val Arg Met Val Arg Gly Trp Gly Leu Gln
50 55 60
Glu His Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Pro Glu Cys Ala Glu Ala Gly Phe Trp Ala Thr
85 90 95
Val Glu Arg Glu Leu Val Gln Ala Phe Thr Gly Leu Ala Ala Gln Glu
100 105 110
Gly Gln Pro Gly Leu Ser Phe Tyr Glu Glu Ser Gly Asp Arg Thr Arg
115 120 125
Phe Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp
130 135 140
His Met Tyr Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro
145 150 155 160
Lys Ala Glu Val Leu Ala Leu Asp Leu Thr Asp Ile Val Pro Phe Thr
165 170 175
Val Arg Leu Asn Ala Ile Val Val Arg Gln Ala Leu Ser Val Ser Val
180 185 190
<210> 251
<211> 206
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 251
Met Asp Ser Gly Ile Ile Ser Asp Glu Arg Lys Asn Asp Tyr Ser Gly
1 5 10 15
Asp Ala Ser Phe Leu Lys Leu Ile Ser Asp Val Asn Pro Glu Tyr Asn
20 25 30
Leu Gln Ser Leu Lys Leu Ile Asp Lys Phe Asn Ser Ile Val Asp Pro
35 40 45
Ile Thr Arg Glu Leu Asp Phe Gly Lys Ile Leu Gln Ile Ala Asn Glu
50 55 60
Asn Pro Asp Leu Val Ile Val Ser Thr Leu Asn His Gln Leu Ala Ser
65 70 75 80
Asn Gln Lys Met Gln Ile Asp Asn Leu Ile Asp Leu Ser Lys Asn Phe
85 90 95
Ile Leu Ser Ala Leu Gly Gly Thr Tyr Leu Asn Glu Gly Ile Gln Lys
100 105 110
Thr Cys Thr Asp Trp Ile Gly Asp Ile Phe Thr Asn Leu Asn Ser Gln
115 120 125
Ile Gln His Ser Trp Ile Ser Phe Tyr Glu Glu Thr Arg Asn Lys Thr
130 135 140
Ser Tyr Arg Tyr Asn Ile Phe Phe Thr Phe Gln Arg Asp Ser Phe Leu
145 150 155 160
Val Val Leu Pro Ile Gly Phe Asp Ile Thr Val His Ala Asn Lys Glu
165 170 175
Thr Val Leu His Leu Lys Pro Thr Asp Ser Ala Ala Tyr Asp Val Ser
180 185 190
Ile Lys Gln Ile Gln Ile Ile Gln Pro Leu Asn Pro Gln Leu
195 200 205
<210> 252
<211> 963
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 252
Met Val Ser Asn Ser Met Val Pro Tyr Asn Val Leu Arg Asn Met Asn
1 5 10 15
Met Pro Thr Ile Ser Gly Thr Arg Trp Asn Lys Glu Met Phe Ser Ser
20 25 30
Ala Ile Asp Asn Thr Ser Val Leu Leu Asp Leu Ile Glu Lys Gly Ile
35 40 45
Asn Asp Gly Asp Asp Leu Leu Gly Leu Phe Ser Phe Ile Gly Leu Thr
50 55 60
Ala Leu Glu Ala Ile Pro Ile Val Gly Gly Thr Leu Ser Lys Leu Ile
65 70 75 80
Ser Ile Ile Phe Phe Ser Ser Lys Ser Ser Ile Asn Pro Gln Lys Ile
85 90 95
Trp Glu Glu Leu Gly Lys Ala Ile Asn Gln Ile Ile Asp Lys Lys Ile
100 105 110
Glu Glu Thr Leu Ile Ser Gly Leu Ile Gln Glu Ile Ser Gly Phe Ala
115 120 125
Asn Val Leu Glu Glu Tyr Arg Ala Ala Tyr Asp Leu Tyr Asn Gly Lys
130 135 140
Ser Val Phe Glu Thr Pro Asp Asn Leu Thr Pro Gly Glu Tyr Leu Val
145 150 155 160
Asn Val Phe Thr Thr Ala Asn Leu Leu Phe Ile Gln Arg Ile Pro Lys
165 170 175
Phe Gln Asp Pro Asp His Asp Val Leu Phe Leu Pro Phe Phe Val His
180 185 190
Ile Ala Glu Met His Ile Leu Leu Ile Arg Asp Ala Ala Met His Gly
195 200 205
Leu Glu Trp Gly Met Asp Glu Lys Ile His Gln Lys Phe Lys Lys Asp
210 215 220
Phe Lys Ser Tyr Ile Gly Glu Tyr Ser Ala Tyr Leu Leu Lys Ile Tyr
225 230 235 240
Lys Lys Gly Leu Lys Glu Ala Ser Glu Arg Glu Leu Asn Asp Lys Asp
245 250 255
Phe Pro Thr Ile Lys Asp Lys Asn His Tyr Ile Asn Thr Val Arg Trp
260 265 270
Asn Val Ile Asn Gln Tyr Lys Arg Gly Met Thr Leu Thr Ala Phe Asp
275 280 285
Phe Ala Tyr Lys Trp Lys Tyr Tyr His Glu Val Tyr Gln Asn Asn Ile
290 295 300
Thr Leu Asn Pro Phe Arg Thr Ile Tyr Ser Asp Ile Ala Gly Ser Val
305 310 315 320
Tyr Pro Tyr Ile Lys Thr Thr Asn Glu Ile Asn Asn Thr Ile Lys Asn
325 330 335
Gln Asn Leu Lys Tyr Arg Gly Ile Phe Lys Lys Leu Gln Val Tyr His
340 345 350
Ala Glu Arg Ile Asp Ser Ile Gln Ser Thr Tyr Ile Arg Asn Asn Glu
355 360 365
Ile Ile Asn Ser Thr Lys Ile Gly Gly Val Gly Gly Arg Ile Ser Ser
370 375 380
Leu Val Leu Glu Glu Pro Ile Lys Asn Pro Leu Ile Gln Val Asn Met
385 390 395 400
Trp Ser Glu Leu Val Pro Ile Ser Leu Gly Phe Lys Phe Tyr Asp Glu
405 410 415
Lys Glu Gln Asn Ile Gly Gly Gly Ser Trp Trp Pro Lys Pro His Lys
420 425 430
Phe Gly Ala Tyr His Phe Val Gly Asn Lys Val Ser Ser Ile Ile Gly
435 440 445
Phe Gly Lys Asn Glu Thr Gly Gly Phe Asn Ser Leu Asp Ala Met Ile
450 455 460
Val Gly Phe Lys Arg Asp Asp Tyr Thr Pro Glu Asn Arg Leu Leu Gly
465 470 475 480
Ile Asn Glu Thr Gly Glu Pro Val Thr Lys Val Ile Asp Ala Gly Asn
485 490 495
Phe Gln Gln Glu Lys Phe Gly Ser Asn Ile Gln Ile Thr Asp Glu Pro
500 505 510
Met Phe Gly Asp Gly Val Leu Gln Phe Thr Asn Tyr Ala Asn Thr Met
515 520 525
Asn Gln Asp Ser Ser Val Thr Tyr Gln Ile Asn Ile Glu Ile Glu Gly
530 535 540
Ile Tyr Gln Leu His Ala Ile Ile Gly Ala Lys Lys Gln Lys Asp Lys
545 550 555 560
Leu Ser Phe Lys Val Ile Leu Asn Thr Lys Gln Gln Lys Lys Ile Ile
565 570 575
Thr Asp Ser Phe Asp Ser His Asp Ile Trp Glu Gly Met Ser Leu Asn
580 585 590
Glu Ser Ile Val Tyr Lys Arg Ile Leu Ile Gly Thr Phe Pro Leu Lys
595 600 605
Arg Gly Met Asn Ser Ile Thr Ile His Asn Asp Val Leu Gln Ser Ser
610 615 620
Ala Asn Ile Lys Thr Trp Asn Leu Gly Ser Leu Glu Leu Thr Met Asp
625 630 635 640
Ser Asp Ser Ile Lys Asp Pro Asp Ile Thr Thr Leu Tyr Asp Lys Asp
645 650 655
Asn Tyr Ala Gly Thr Lys Lys Tyr Ile Phe Glu Asp Thr Ala Arg Leu
660 665 670
Ser Asp Phe Asn Asp Arg Thr Ser Ser Ile Lys Val Gly Ser His Ile
675 680 685
Ala Gly Val Arg Phe Tyr Glu His Tyr His Tyr Ser Gly Asp Phe Ile
690 695 700
Asp Leu Gly Asn Gly Glu Lys Leu Ser Leu Lys Asn His Arg Leu Asn
705 710 715 720
Lys Arg Leu Ser Ser Val Lys Phe Ala Asn Leu Gly Leu Tyr Lys Glu
725 730 735
Asp Asn Tyr Lys Gly Glu Lys Lys Leu Ile Phe Glu Asp Ile Pro Tyr
740 745 750
Leu Thr Asp Phe Asn Asp Lys Thr Ser Ser Ile Val Val Ser Pro Asn
755 760 765
Val Pro Gly Thr Val Arg Leu Phe Glu His Ser Tyr Tyr Asn Gly Asp
770 775 780
Tyr Ile Glu Val Ser Gly Gly Gln Lys Leu Ser Leu Lys Gly His Asn
785 790 795 800
Leu Asn Arg Lys Ile Ser Ser Ile Lys Phe Leu Lys Glu His Gln Val
805 810 815
Phe Asn Gly Leu Tyr Glu Ile Val Thr Ala Ile Asn Lys Lys Ser Val
820 825 830
Val Asp Trp Ser Gln Asp Lys Asn Ser Asn Val His Leu Trp Glu Gln
835 840 845
Arg Asp Arg Asp Asn Gln Lys Trp Tyr Phe Asp Tyr Asp Ser Ser Lys
850 855 860
Gln Ala Phe Gln Ile Lys Ser Lys Ala Asp Lys Asn Lys Val Leu Ala
865 870 875 880
Trp Asn Asp Phe Gly Gly Ser Lys Asn Val Phe Ala Thr Pro Asn Leu
885 890 895
Lys Lys Asp Glu His Phe Trp Thr Phe Asp Tyr Leu Gly Glu Gly Asn
900 905 910
Tyr Leu Ile Arg Asn Lys Lys Asp Gln Asn Leu Val Leu Asp Val Asp
915 920 925
Gly His Gln Thr Ala Asn Gly Thr Asn Ile Lys Val Asn Ala Gln His
930 935 940
Ala Leu Asp Asn Pro Ser Ile Glu Ala Gln Lys Phe Lys Leu Asn His
945 950 955 960
Val Ile Asn
<210> 253
<211> 192
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 253
Met Ala Ala Ser Phe Arg Thr Val Val Glu Val Gly Pro Asp His Leu
1 5 10 15
Glu Arg Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Phe Asp Phe Glu Ala Ile Arg Ala Ala Ala Ala Ala
35 40 45
Val Pro Asp Thr Thr Val Val Arg Gln Val Arg Gly Trp Gly Leu Gln
50 55 60
Glu His Ala Pro Val Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Pro Glu Tyr Ala Glu Ala Gly Phe Trp Gly Thr
85 90 95
Val Glu Arg Glu Leu Val Glu Ala Phe Thr Gly Leu Ala Gly Gln Glu
100 105 110
Gly Gln Pro Gly Leu Ser Phe Tyr Glu Glu Ser Asp Gln Arg Thr Arg
115 120 125
Phe Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Glu Thr Gly Asp
130 135 140
Asp Ile Tyr Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro
145 150 155 160
Lys Ala Glu Ala Thr Thr Met Glu Leu Thr Asp Val Val Pro Phe Ala
165 170 175
Val Arg Leu Asn Ala Ile Leu Val Arg Gln Ala Pro Thr Val Ala Val
180 185 190
<210> 254
<211> 483
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 254
Met Arg Gly Asp Arg Leu Pro Ala Arg Pro Arg Pro Arg Gln His Val
1 5 10 15
Leu Pro Ala Arg Leu Arg Ala Arg Gly Asp Ala Leu Glu Val Gly Ala
20 25 30
Asp Leu Gly Ala Ala Ala Ala Arg Arg Ala Ala Gln Ala Arg Ala Gly
35 40 45
His Ala Arg Arg Gly Leu Arg Ala Ala Glu Gly Arg Arg Arg Arg Arg
50 55 60
Arg Ala Pro Ala Ala Ala Gly Arg Arg Arg Gly Pro Glu Arg Asp Pro
65 70 75 80
Ala Val Arg Gly Asp Glu Arg Gly Asp Pro Ala Gln Gly Pro Pro Leu
85 90 95
Arg Arg Gly Arg Gly Ala Gly Pro Arg Gly Pro Gly Gly Gly Ala Gly
100 105 110
Ala Ala Pro Ala Arg Arg His Pro Arg Leu Gly Gly Ala Ala Arg Ala
115 120 125
Ala Arg Val Pro Ala Ala Arg Asp Asp Arg Arg Arg Gly Arg Ala Gly
130 135 140
Arg Arg Arg Gly Gly Thr Ala Arg Arg Pro Ala Val Ala Gly Ala Ala
145 150 155 160
Ala Pro Pro Gln Gly Pro His Val Pro His Asp Arg Arg Cys Ala Ala
165 170 175
Val Ala Gly Arg Ala Leu Leu Ala Glu Gly Arg Gly Gly Pro Arg Pro
180 185 190
Gly Gly Gly Val His Arg Asp Arg Ala Gly Pro Asp Gly Gln Gln His
195 200 205
Pro Gly Ala Ala Arg Gly Gly Pro Glu Gly Ala Glu Gly Arg Glu Gly
210 215 220
Gly Pro Glu Ala Arg Glu Glu Gly Gly Gly Gly Glu Ala Gly Pro Val
225 230 235 240
Pro Glu Ala Arg Glu Ala Arg Gln Gly Gln Gly Arg Arg Cys Arg Arg
245 250 255
Ala Arg Arg Glu Ala Pro Pro Arg Ala Leu Ser Gly Ala Leu Gly Ile
260 265 270
Leu Leu Leu Met Ile Asp Cys Pro Leu Arg Arg Ala Thr Arg Glu Gln
275 280 285
Glu Lys Pro Leu Ala Ala Ser Phe Arg Thr Val Val Glu Val Ala Pro
290 295 300
Asp Arg Leu Asp Glu Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala
305 310 315 320
Ile Ala Pro Ala Thr Val Asn Phe Asp Phe Glu Ala Ile His Arg Ala
325 330 335
Ala Ala Ala Val Pro Asp Ser Thr Ile Val Arg Met Val Arg Gly Trp
340 345 350
Gly Leu Lys Glu Tyr Ala Pro Leu Leu Val Met Val Leu Ser Leu Lys
355 360 365
Glu Ala Val Arg Gln Ala Leu Pro Ala Glu Cys Ala Glu Ala Gly Phe
370 375 380
Trp Glu Thr Val Glu Arg Glu Leu Val Ala Ala Phe Thr Gly Leu Gly
385 390 395 400
Glu Gln Glu Gly Gln Pro Gly Leu Ser Phe Tyr Glu Glu Ser Pro Glu
405 410 415
Arg Thr Arg Tyr Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Gly
420 425 430
Ala Gly Gly Ala Leu His Ala Leu Ala Phe Cys Val Asp Val Thr Val
435 440 445
Glu Leu Pro Lys Glu Arg Val Leu Gly Leu Gly Leu Thr Asp Thr Ala
450 455 460
Pro Phe Ala Val Gly Leu Asn Ala Ile Val Leu Arg Arg Pro Leu Ala
465 470 475 480
Val Ala Ala
<210> 255
<211> 192
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 255
Met Ala Ala Ser Phe Arg Thr Val Val Glu Val Ala Pro Asp Arg Leu
1 5 10 15
Asp Glu Ala Arg Ser Ile Asp Arg Val Phe Gln Glu Ala Ile Ala Pro
20 25 30
Ala Thr Val Asn Phe Asp Phe Glu Ala Ile His Arg Ala Ala Ala Ala
35 40 45
Val Pro Asp Ser Thr Ile Val Arg Met Val Arg Gly Trp Gly Leu Lys
50 55 60
Glu Tyr Ala Pro Leu Leu Val Met Val Leu Ser Leu Lys Glu Ala Val
65 70 75 80
Arg Gln Ala Leu Pro Ala Glu Cys Ala Glu Ala Gly Phe Trp Glu Thr
85 90 95
Val Glu Arg Glu Leu Val Ala Ala Phe Thr Gly Leu Gly Glu Gln Glu
100 105 110
Gly Gln Pro Gly Leu Ser Phe Tyr Glu Glu Ser Pro Glu Arg Thr Arg
115 120 125
Tyr Tyr Arg Asp Leu Phe Phe Ala Leu Gln Asp Glu Gly Ala Gly Gly
130 135 140
Ala Leu His Ala Leu Ala Phe Cys Val Asp Val Thr Val Glu Leu Pro
145 150 155 160
Lys Glu Arg Val Leu Gly Leu Gly Leu Thr Asp Thr Ala Pro Phe Ala
165 170 175
Val Gly Leu Asn Ala Ile Val Leu Arg Arg Pro Leu Ala Val Ala Ala
180 185 190
<210> 256
<211> 355
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 256
Met Ala Pro Ala Lys Asn Ala Tyr His Ser Ile His Leu Ala Ala Asn
1 5 10 15
Ala Asn Leu Lys Trp Asp Val Tyr Gly Asn Ser Pro Asn Asp Glu Ala
20 25 30
Ala Ile Leu Leu Tyr Asn Gly Ser Phe Gly Asp Asn Glu Gln Phe Val
35 40 45
Phe Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser
50 55 60
Gly Lys Ala Val Thr Ile Gly Gly Gly Trp Ala Ala Pro Thr Asp Phe
65 70 75 80
Thr Tyr Asn Arg Glu Gly Leu Lys Gln Ser Lys Trp Thr Gly Ala Ala
85 90 95
Asp Asn Lys Trp Tyr Leu Arg Asp Gln Gly Asn Asn Asn Tyr Glu Ile
100 105 110
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Arg Ile Gly Ile
115 120 125
Thr Gly Pro Gln Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn
130 135 140
Asp Arg Leu Phe His Ile Pro Ala Ala Ala Arg Ser Thr Phe Ser Leu
145 150 155 160
Pro Thr Leu Pro Thr Ile Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr
165 170 175
Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val
180 185 190
Gly Ala Ser Leu Leu Pro Cys Ile Met Val Asn Asp Ser Gln Val Asp
195 200 205
Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu
210 215 220
Glu Tyr Trp Asp Lys Thr Phe Ser Lys Val Val Gln Pro Gly Leu Ser
225 230 235 240
Glu Ser Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys
245 250 255
Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ala Asp Leu Gly Leu Lys
260 265 270
Phe Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile
275 280 285
Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg
290 295 300
Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln
305 310 315 320
Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Thr
325 330 335
Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg
340 345 350
Lys Thr Ser
355
<210> 257
<211> 347
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 257
Met Ser Glu Ile Glu Gln Thr Thr Thr Ser Asp Met Ser Leu Ser Val
1 5 10 15
Asn Glu Glu Thr Met Lys Lys Ala Leu Gln Asp Ala Gly Leu Phe Ala
20 25 30
Lys Ser Met Asn Val Tyr Ser Tyr Leu Leu Ile Asn Asn Pro Asn Val
35 40 45
Asn Phe Glu Arg Ile Asp Val Glu Gly Asn Thr Asn Leu Pro Asn Gln
50 55 60
Ile Val Gln Asp Gln Lys Asn Ala Arg Glu His Ala Val Lys Trp Asp
65 70 75 80
Thr Gln Ile Lys Arg Gln Leu Leu Asn Thr Leu Thr Gly Ile Val Gln
85 90 95
Tyr Asp Thr Thr Phe Asp Asn Tyr Tyr Glu Val Leu Val Asp Ala Met
100 105 110
Asn Asp Gly Asp Gly Asp Thr Leu Lys Glu Gly Ile Thr Asp Leu Gln
115 120 125
Gly Glu Ile His Gln Asn Arg Glu His Thr Gln Lys Leu Ile Gln Glu
130 135 140
Leu Thr Lys Leu Lys Asp Asp Ile Gly Gln Asp Val Arg Ala Phe Gly
145 150 155 160
Asp His Thr Asp Leu Leu Gln Ser Ile Leu Lys Lys Gln Ala Ala Glu
165 170 175
Ile Glu Glu Asp Gln Lys Arg Leu Asp Glu Val Leu Lys Ser Val Asn
180 185 190
Tyr Tyr Lys Lys Leu Glu Ser Asp Gly Phe Asn Val Met Lys Gly Thr
195 200 205
Val Leu Gly Leu Pro Leu Ile Gly Gly Ile Ile Val Val Val Ala Lys
210 215 220
Asp Asn Leu Gly Lys Leu Glu Pro Val Ser Gln Gln Leu Leu Glu Lys
225 230 235 240
Leu Ser Gln Pro Val Asp His Lys Val Thr Leu Asn Arg Val Val Gly
245 250 255
Val Thr Tyr Asn Asn Ile Thr Glu Met His Asn Ala Ile Asp Thr Ala
260 265 270
Ile Asn Ala Leu Thr Tyr Met Ser Ala Gln Trp Asn Asp Leu Asp Ser
275 280 285
Lys Tyr Thr Ala Val Leu Gln His Ile Glu Asn Ala Ser Lys Lys Asp
290 295 300
Asp Pro Asn Lys Phe Lys Phe Leu Glu Pro Asn Leu Lys Ala Ala Lys
305 310 315 320
Asp Ser Trp Lys Thr Phe Lys Thr Asp Ala Ile Thr Leu Lys Glu Gly
325 330 335
Ile Lys Glu Leu Lys Val Glu Pro Leu Gln Lys
340 345
<210> 258
<211> 427
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 258
Met Phe Leu Ile Asn Gly Ala Val Ile Phe Leu Asn Arg Ile Tyr Met
1 5 10 15
Glu Ile Asn Met Arg Gln Asp Leu Gly Lys Pro Ile Arg Ala Val Ala
20 25 30
Tyr Glu Ala Val Ala Glu Leu Glu Gly Ile Arg Thr Val Ser Glu Ala
35 40 45
Ala Lys Tyr Val Pro Val Gln Tyr Phe Gly Val Met Ala Gly Lys Asp
50 55 60
Ala Pro Asp Tyr Gln Asp Gly Thr Phe Ile Val Lys Lys Ser Asn Ile
65 70 75 80
Val Ala Ile Lys Arg Tyr Val Asn Ser Ser Leu Arg Leu Pro Val Asp
85 90 95
Leu Thr Leu Val Glu Arg Met Leu Gly Tyr Thr Arg Val Asp Ser Glu
100 105 110
Gly Leu Glu Pro Arg Asp Ile Gln Ser Leu His Gln Glu Ile His Lys
115 120 125
His Ala Leu Ser Trp Gly Thr Leu Glu Arg Glu Thr Lys Asn Leu Gly
130 135 140
Gly Arg Leu Asp Leu Phe Ser Lys Gly Phe Ile Asn Thr Gly Glu Ile
145 150 155 160
Val Ile Asp His Leu Lys Ser Phe Thr Gly Tyr Arg Asn Leu Leu Gly
165 170 175
Thr Ile Asp Thr Leu Thr Glu Lys Glu Met Glu Ala Leu Ala Ala Ile
180 185 190
Pro Leu Gly Asn Ser Asp Thr Glu Lys Val Gln Ser Leu Ala Arg Leu
195 200 205
Phe Thr Arg Met Lys Ala Asp Ile Glu Ser Phe Tyr Asn Arg Val Ile
210 215 220
Ala Val Lys Ala Leu Ala Ser Glu Phe Ser Arg Lys Ile Thr Glu Asp
225 230 235 240
Leu Leu Pro Val Ile Lys Val Lys Met Arg Asn Ala Glu Met Ala Gly
245 250 255
Ile Glu Lys Asp Ala Arg Val Glu Lys Ile Lys Phe Glu Leu Glu Glu
260 265 270
Leu Asp Gln Val Val Ala Glu Lys Leu Glu Glu Tyr Arg Ser Leu Val
275 280 285
Gly Tyr Ser Phe Thr Gly Leu Val Phe Gly Pro Ile Gly Val Ala Ile
290 295 300
Thr Gly Gly Ile Phe Gly Ser Gln Ala Glu Arg Val Arg Gly Glu Lys
305 310 315 320
Asn Ala Leu Leu Glu Arg Arg Thr Glu Leu Leu Glu Gln Met Arg Gly
325 330 335
Ala Asn Met Ser Lys Leu Leu Asn Glu Leu Ala Gly Gly Leu Val Asp
340 345 350
Ile Lys Thr Leu Val Glu Asp Ala Glu Lys Gly Ala Lys Asn Leu Glu
355 360 365
Asp Val Trp Ala Phe Ile Trp Leu Tyr Ile Asp Glu Ser Ser Ala Arg
370 375 380
Leu Ser Glu Ala Gln Thr Ala Leu Asp Leu Asn Met Leu Val Leu Asp
385 390 395 400
Leu Gln Ser Val Ile Asp Pro Trp Arg Val Ile His Gly His Ala Asn
405 410 415
Ala Leu Ser Arg Val Phe Asn Glu Val Ala Asp
420 425
<210> 259
<211> 402
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 259
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ile Ser Glu Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Asn Ile Ala Leu Lys Ser Asn Thr Lys Lys Val
35 40 45
Trp Asn Val Ser Gln Gly Ser Asn Asp Asn Asp Arg Ala Ile Val Leu
50 55 60
Trp Gln Gly Gly Ser Ala Asp His Glu Arg Phe Ala Phe Phe Gln Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Val Val
85 90 95
Thr Phe Gly Thr Gly Ser Trp Val Ser Gly Gly Pro Ile Pro Asp Ser
100 105 110
Glu Thr Leu Gln Gln Gln Lys Trp Thr Gly Glu Ala Lys Gln Lys Trp
115 120 125
Asn Leu Arg Asp Leu Gly Ser Asp Gly His Gly Ser Asn Asn Tyr Glu
130 135 140
Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg Trp Ile Gly
145 150 155 160
Ser Thr Val Asp Asn Thr Glu Tyr Leu Lys Leu Asp Asn Ser Asn Pro
165 170 175
Ser Asp Ala Asp Lys Val Phe Thr Leu Phe Asn Gly Thr Met Asn Leu
180 185 190
Trp Gly Gln Asn Ile Thr Phe Phe Asn Asp Thr Ile Ser Val Pro Lys
195 200 205
Leu Pro Ala Thr Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly
210 215 220
Val Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala
225 230 235 240
Thr Leu Ile Pro Cys Phe Met Val Asn Asp Asn Gln Ala Ser Asp Tyr
245 250 255
Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr
260 265 270
Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg Asn
275 280 285
Tyr Ser Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln Lys Met Thr
290 295 300
Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe Gly
305 310 315 320
Asp Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Lys Thr Ile Gln Thr
325 330 335
Glu Ile Ser Thr Thr Asn Thr Glu Ala Ser Glu Glu Thr Ile Thr Ser
340 345 350
Thr Val Thr Ser Glu Ala Gly Lys Thr Thr Gly Tyr Thr Glu Tyr Gln
355 360 365
Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val Ser
370 375 380
Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile Ala Arg Lys Asn
385 390 395 400
Ala Gln
<210> 260
<211> 373
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 260
Met Pro Glu Asn Asn Arg Tyr Tyr Asn Ile Ala Leu Lys Ser Asn Thr
1 5 10 15
Lys Lys Val Trp Asn Val Ser Gln Gly Ser Asn Asp Asn Asp Arg Ala
20 25 30
Ile Val Leu Trp Gln Gly Gly Ser Ala Asp His Glu Arg Phe Ala Phe
35 40 45
Phe Gln Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Val Val Thr Phe Gly Thr Gly Ser Trp Val Ser Gly Gly Pro Ile
65 70 75 80
Pro Asp Ser Glu Thr Leu Gln Gln Gln Lys Trp Thr Gly Glu Ala Lys
85 90 95
Gln Lys Trp Asn Leu Arg Asp Leu Gly Ser Asp Gly His Gly Ser Asn
100 105 110
Asn Tyr Glu Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg
115 120 125
Trp Ile Gly Ser Thr Val Asp Asn Thr Glu Tyr Leu Lys Leu Asp Asn
130 135 140
Ser Asn Pro Ser Asp Ala Asp Lys Val Phe Thr Leu Phe Asn Gly Thr
145 150 155 160
Met Asn Leu Trp Gly Gln Asn Ile Thr Phe Phe Asn Asp Thr Ile Ser
165 170 175
Val Pro Lys Leu Pro Ala Thr Gly Thr Arg Pro Asp Ala Pro Asn Tyr
180 185 190
Thr Gly Gly Val Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val
195 200 205
Val Gly Ala Thr Leu Ile Pro Cys Phe Met Val Asn Asp Asn Gln Ala
210 215 220
Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys
225 230 235 240
Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu
245 250 255
Thr Arg Asn Tyr Ser Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln
260 265 270
Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu
275 280 285
Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Lys Thr
290 295 300
Ile Gln Thr Glu Ile Ser Thr Thr Asn Thr Glu Ala Ser Glu Glu Thr
305 310 315 320
Ile Thr Ser Thr Val Thr Ser Glu Ala Gly Lys Thr Thr Gly Tyr Thr
325 330 335
Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser
340 345 350
Ile Val Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile Ala
355 360 365
Arg Lys Asn Ala Gln
370
<210> 261
<211> 360
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 261
Met Ala Pro Ala Asn Asn Ala Tyr His Ser Ile His Leu Ala Ala Asp
1 5 10 15
Ala Thr Leu Lys Trp Asp Val Tyr Gly Asn Ser Pro Asn Asp Glu Ala
20 25 30
Gly Ile Leu Leu Tyr Asn Gly Asn Phe Ser Asp Asn Glu Gln Phe Leu
35 40 45
Phe Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser
50 55 60
Gly Lys Ala Val Thr Ile Gly Ala Gly Phe Ile Thr Pro Thr Asp Tyr
65 70 75 80
Ser Tyr Asn Arg Glu Gly Leu Lys Gln Ser Lys Trp Thr Gly Ala Ala
85 90 95
Ala Asp Lys Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile
100 105 110
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile
115 120 125
Ser Gly Gln Lys Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn
130 135 140
Asp Arg Leu Phe His Ile Pro Ala Ala Ala Arg Ser Thr Phe Ser Leu
145 150 155 160
Pro Thr Leu Pro Thr Ile Gly Thr Arg Pro Asp Ala Pro Asp Tyr Arg
165 170 175
Glu Asp Phe Pro Gly Arg Ile Thr Gln Gln Leu Pro Gln Thr Ser Asn
180 185 190
Ser Val Val Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Asn Asp
195 200 205
Asn Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr
210 215 220
Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Thr Val Leu Pro
225 230 235 240
Ala Gly Tyr Thr Lys Asn Tyr Ser Phe Lys Thr Gly Met Thr Ser Val
245 250 255
Asp Gln Gln Arg Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp
260 265 270
Phe Gly Leu Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Thr Glu Ile
275 280 285
Ser Arg Thr Ile Gln Thr Glu Ile Thr Ser Thr Ser Thr Glu Ala Ser
290 295 300
Glu Glu Thr Ile Thr His Asn Val Thr Ser Glu Ala Gly Lys Thr Thr
305 310 315 320
Ala Phe Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala
325 330 335
Asp Gly Ser Ile Val Ser Asp Pro Trp Val Val Lys Asn Asn Lys Asn
340 345 350
Thr Ile Val Arg Lys Ile Glu Lys
355 360
<210> 262
<211> 364
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 262
Met Arg Ser Leu Tyr Ile Asn Phe Tyr Lys Lys Cys Phe Leu Ser Ile
1 5 10 15
Met Ile Ala Gly Val Gly Met Ser Asn Val Ile Pro Leu Lys Pro Leu
20 25 30
Ala Glu Gly Phe Glu Glu Gly Gln Gln Leu Gln Gln Lys Ser Ala Glu
35 40 45
Ser Tyr Ser Leu Gly Pro Ala Gly Phe Lys Asp Val Met Ala Gln Thr
50 55 60
Thr Thr Ser Val Phe Ala Met Asp Ser Tyr Ala Lys Leu Ile Arg Asn
65 70 75 80
Gln Gln Glu Thr Asp Leu Asn Lys Ile Ser Ser Ile Asn Asp Asn Leu
85 90 95
Arg Leu Lys Met Ile Gln His Gln Lys Asp Ala Lys Ile Asn Ala Ala
100 105 110
His Trp Leu Glu Lys Met Lys Pro Gln Ile Met Lys Thr Asn Gln Asn
115 120 125
Ile Ile Asn Tyr Asn Asp Val Phe Gln Thr Asn Tyr Asn Ser Leu Leu
130 135 140
Lys Ala Ile Asp Gln Lys Asp Ser Gly Lys Leu Lys Glu Gly Leu Glu
145 150 155 160
Glu Leu Tyr Ala Ser Ile Leu Lys Asn Glu Lys Glu Val Asp Val Leu
165 170 175
Leu Gly Asp Leu Lys Thr Phe Arg Asn Arg Met Glu Arg Asp Thr Thr
180 185 190
Ser Phe Lys Asn Asp Ser Asn Gln Leu Asn Ser Ile Leu Ala Ser Thr
195 200 205
Asn Ala Gly Ile Pro Val Leu Gln Gln Gln Ile Asn Ala Tyr Asn Asp
210 215 220
Leu Ile Lys Lys Ser Asn Asn Met Val Ile Thr Gly Gly Ile Leu Cys
225 230 235 240
Ser Ser Leu Leu Ala Cys Ile Ala Gly Gly Pro Met Ile Ala Asn Ala
245 250 255
Leu Asn Glu Ile Ala Arg Ala Glu Arg Glu Ile Ala Asn Leu Lys Glu
260 265 270
Lys Ile Ser Gly Ala Gln Lys Asp Val Val Ile Leu Thr Asp Ile Asn
275 280 285
Ile Lys Thr Ala Asn Met Thr Asn Thr Ile Asp Ala Ala Ile Thr Ala
290 295 300
Leu Gln Lys Ile Ser Asn Gln Trp His Thr Val Gly Ala Lys Tyr Asn
305 310 315 320
Asn Leu Leu Lys Asn Val Lys Asp Ile Ser Pro Glu Glu Phe Ala Phe
325 330 335
Ile Lys Glu Asp Leu Asn Thr Ala Arg Asp Ser Trp Lys Asp Ile Lys
340 345 350
Asp Tyr Thr Glu Lys Leu Asn Glu Gly Val Ser Lys
355 360
<210> 263
<211> 558
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 263
Met Glu Glu Asn Ser Ile Ser Leu Gln Gln Val Thr Gly Ala Gln Gln
1 5 10 15
Met Gln Ala Ser Thr Tyr Ser Ala Gly Gly Ser Thr Ser Gln Thr Ala
20 25 30
Glu Ser Thr Ser Thr Leu Lys Ser Leu Leu Thr Met Asp Asn Leu Lys
35 40 45
Lys Val Gly Thr Leu Thr Gly Asp Val Leu Lys Thr Ala Tyr Lys Asp
50 55 60
Ala His Glu Asn Asn Gly Asn Tyr Asn Asn Thr Phe Arg Asp Leu Ala
65 70 75 80
Met Gly Gly Thr Ser Leu Ile Pro Tyr Gly Gly Val Leu Val Ser Gly
85 90 95
Leu Ile Gly Leu Ile Trp Pro Val Asn Thr Asp Ala Gln Lys Asn Gln
100 105 110
Met Gln Lys Leu Ile Asp Asn Leu Asn Asn Met Met Asp Ser Lys Ile
115 120 125
Thr Ala Asn Glu Met Ala Asn Ile Gln Ser Glu Val Asn Thr Leu Lys
130 135 140
Glu Arg Leu Gln Arg Phe Glu Lys Asn Ile Asn Met Ser Ile Ser Arg
145 150 155 160
Ser Val Tyr Arg Leu Ala Thr Asp Lys Glu Gln Ala Leu Ala Thr Glu
165 170 175
Ala Ser Ala Ile Ser Asn Asp Phe Ser Lys Ile Leu Gly His Ser Lys
180 185 190
Gln Glu Leu Gln Thr Ile Pro Ala Leu Pro Val Tyr Ala Gln Leu Ala
195 200 205
Thr Ala His Ile Glu Phe Leu Gln Phe Leu Ala Lys Asn Gly Thr Gly
210 215 220
Ser Lys Leu Gln Tyr Thr Thr Ala Ala Leu Asn Thr Leu Leu Tyr Gly
225 230 235 240
Glu Pro Asp Glu Thr Phe Glu Gln Arg Met His Lys Leu Ala Glu Lys
245 250 255
Tyr Arg Glu His Val Asn Thr Thr Tyr Lys Leu Ala Asp Glu Lys Met
260 265 270
Asn Val Lys Ile Arg Asp Ile Ala Asn Asn Ser Pro Val His His Met
275 280 285
Asn Asn Tyr Glu Thr Val Asp Glu Met Met Gly Ala Met Gln Val Tyr
290 295 300
Met Leu Asn Met His Pro Ala Ala Asn Asn Tyr Asp Ser Met Lys Lys
305 310 315 320
Lys Leu Asn Glu Asp Val Thr Ala Leu Leu Asp Leu Lys Thr Lys Lys
325 330 335
Gln Glu Leu Leu Ala Ser Thr Val Gly Asn Glu Ala Phe Gln Lys Ile
340 345 350
Val Ile Gly Glu Trp Asp Ala His Asn His Val Ala Asp Leu Asn His
355 360 365
Ile Gln Gln Thr Gly Trp Ala Tyr Leu Lys Asn Asn Trp Tyr Tyr Leu
370 375 380
Ser Pro Asp Asp Ser Thr Lys Asn Leu His Gly Asp Thr Phe Gln Arg
385 390 395 400
Gly Gln Gln Val Thr Gly Gln Ile Glu Ile Asp His Lys Thr Tyr Phe
405 410 415
Phe Lys Pro Asp Thr Gly Gln Met Val Thr Arg Trp Leu Gln Ile Gly
420 425 430
Asp Lys Phe Phe Tyr Ile Ser Pro Tyr Asp Gly Phe Lys Asn Trp Asp
435 440 445
Gly Val Glu Phe Lys Gln Gly Glu Leu Val Thr Gly Trp Leu Glu Leu
450 455 460
Gly Asp Arg Ala Thr Tyr Tyr Leu Ser Pro Glu Asn Asp Lys Lys Asn
465 470 475 480
Asp Glu Asn Lys Asn Ser Lys Thr Phe Asp Lys Gly Gln Met Met Thr
485 490 495
Gly Trp Ile Leu Val Lys Asn Val Ser Tyr Tyr Met Asp Asn Lys Glu
500 505 510
Gly Asn Lys Asp Phe Gln Asp Val Lys Gly Arg Met Leu Arg Gly Thr
515 520 525
Asn Lys Gly Lys Gly Phe Arg Leu Asn Ser Gly Lys Ser Pro Asp Asp
530 535 540
Phe Lys Thr His Lys Phe Asn Asp Asn Gly Thr Trp Ile Asn
545 550 555
<210> 264
<211> 359
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 264
Met Ala Thr Pro Glu Lys Asn Gly Tyr Tyr Ser Ile His Leu Ala Ser
1 5 10 15
Asn Ala Trp Lys Lys Trp Asp Val Val Gln Ser Ser Leu Asp Asn Glu
20 25 30
Arg Gly Ile Leu Leu Tyr Asp Val Ser Gly Arg Ile Pro Asp Asn Gln
35 40 45
Gln Phe Ala Phe Phe Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn
50 55 60
Lys Asn Ser Gly Lys Pro Val Thr Ile Gly Asp Asn Val Leu Pro Met
65 70 75 80
Ala Gly Leu Gly Gly Asn Ser Gly Ala Leu Lys Gln Asn Asn Trp Thr
85 90 95
Gly Asn Ala Asn Glu Lys Trp Tyr Leu Arg Asp Leu Gly Ser Asn Asp
100 105 110
Phe Glu Ile Val Asn Gln Gly Thr Gly Lys Val Ala Ser Tyr Ala Trp
115 120 125
Val Gly Asp Leu Val Arg Asn Tyr Glu Tyr Val Asp Leu Asp Asn Ala
130 135 140
Asn Pro Ser Asp Lys Asp Arg Val Phe His Ile Pro Ala Ala Arg Ser
145 150 155 160
Thr Phe Thr Val Pro Thr Leu Pro Ala Thr Gly Thr Arg Pro Val Ala
165 170 175
Pro Asn Tyr Thr Gly Gly Ile Asp Glu Gln Leu Pro Gln Thr Ser Glu
180 185 190
Ser Val Ile Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Lys Asp
195 200 205
Ser Gln Thr Asn Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr
210 215 220
Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln
225 230 235 240
Pro Gly Leu Ser Glu Ala Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val
245 250 255
Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ala Asp
260 265 270
Phe Gly Ile Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile
275 280 285
Ser Arg Thr Ile Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr
290 295 300
Glu Glu Thr Arg Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr
305 310 315 320
Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala
325 330 335
Asp Gly Ser Ile Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile
340 345 350
Thr Val Thr Arg Lys Thr Ser
355
<210> 265
<211> 390
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 265
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Gly Val Gly Ala
1 5 10 15
Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala
20 25 30
Asn Asn Glu Tyr His Thr Ile His Leu Ala Ala Asn Thr Asn Ile Ala
35 40 45
Trp Asp Val Tyr Lys Gly Trp Gln Ser Asp Asp Ala Gly Ile Val Leu
50 55 60
Trp Asp Gly Ala Arg Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Gly Ser Ala Ile Ile Gly Ser Asp Gly Val Arg Asn Phe Pro
100 105 110
Ser Phe Asn Asn Glu Gly Leu Arg Gln Thr Ser Trp Thr Gly Ala Pro
115 120 125
Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Phe Glu Ile
130 135 140
Val Asn Gln Gly Ser Gly Lys Val Ala Ser Trp Ala Gly Val Glu Met
145 150 155 160
Tyr Gly Tyr Lys Asp Tyr Val Asp Leu Asp Glu Ser Asn Pro Ser Asp
165 170 175
Lys Asp Arg Leu Phe Asn Ile Pro Ala Ala Arg Ser Thr Phe Ser Leu
180 185 190
Pro Thr Leu Pro Ala Val Gly Thr Arg Pro Asn Ala Pro Asp Tyr Asn
195 200 205
Thr Ser Gly Ala Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val
210 215 220
Val Val Gly Ala Ser Leu Ile Pro Ser Ile Met Val Lys Asp Asn Gly
225 230 235 240
Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Val Leu Val
245 250 255
Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu Ile Ile Pro Ala Gly
260 265 270
Ala Thr Ser Lys Tyr Thr Val Lys Ser Gly Val Ser Glu Ile Asp Gln
275 280 285
Lys Lys Met Ser Asp Thr Leu Ala Met Ser Phe Gly Ser Asp Leu Gly
290 295 300
Leu Lys Phe Gly Glu Ser Ser Leu Ser Ile Lys Ser Ser Val Ser Arg
305 310 315 320
Thr Ile Gln Thr Glu Val Thr Lys Thr Ser Thr Asn Ala Thr Glu Glu
325 330 335
Thr Glu Glu Lys Ser Ile Pro Ser Ile Pro Gly Lys Asn Thr Gly Phe
340 345 350
Thr Ala Tyr Gln Leu Val Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly
355 360 365
Ser Val Val Thr Asp Pro Ser Ile Val Arg Asp Ser Lys Gln Thr Ile
370 375 380
Ala Arg Thr Ile Ala Asn
385 390
<210> 266
<211> 363
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 266
Met Ala Ala Pro Ala Asn Asn Glu Tyr His Thr Ile His Leu Ala Ala
1 5 10 15
Asn Thr Asn Ile Ala Trp Asp Val Tyr Lys Gly Trp Gln Ser Asp Asp
20 25 30
Ala Gly Ile Val Leu Trp Asp Gly Ala Arg Gly Asp Asn Glu Gln Phe
35 40 45
Val Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn
50 55 60
Ser Gly Lys Pro Val Gly Ile Gly Ser Ala Ile Ile Gly Ser Asp Gly
65 70 75 80
Val Arg Asn Phe Pro Ser Phe Asn Asn Glu Gly Leu Arg Gln Thr Ser
85 90 95
Trp Thr Gly Ala Pro Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn
100 105 110
Asn Asn Phe Glu Ile Val Asn Gln Gly Ser Gly Lys Val Ala Ser Trp
115 120 125
Ala Gly Val Glu Met Tyr Gly Tyr Lys Asp Tyr Val Asp Leu Asp Glu
130 135 140
Ser Asn Pro Ser Asp Lys Asp Arg Leu Phe Asn Ile Pro Ala Ala Arg
145 150 155 160
Ser Thr Phe Ser Leu Pro Thr Leu Pro Ala Val Gly Thr Arg Pro Asn
165 170 175
Ala Pro Asp Tyr Asn Thr Ser Gly Ala Ile Asp Gln Gln Leu Pro Gln
180 185 190
Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro Ser Ile Met
195 200 205
Val Lys Asp Asn Gly Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro
210 215 220
Tyr Tyr Val Leu Val Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu
225 230 235 240
Ile Ile Pro Ala Gly Ala Thr Ser Lys Tyr Thr Val Lys Ser Gly Val
245 250 255
Ser Glu Ile Asp Gln Lys Lys Met Ser Asp Thr Leu Ala Met Ser Phe
260 265 270
Gly Ser Asp Leu Gly Leu Lys Phe Gly Glu Ser Ser Leu Ser Ile Lys
275 280 285
Ser Ser Val Ser Arg Thr Ile Gln Thr Glu Val Thr Lys Thr Ser Thr
290 295 300
Asn Ala Thr Glu Glu Thr Glu Glu Lys Ser Ile Pro Ser Ile Pro Gly
305 310 315 320
Lys Asn Thr Gly Phe Thr Ala Tyr Gln Leu Val Thr Lys Tyr Thr Leu
325 330 335
Lys Arg Thr Asp Gly Ser Val Val Thr Asp Pro Ser Ile Val Arg Asp
340 345 350
Ser Lys Gln Thr Ile Ala Arg Thr Ile Ala Asn
355 360
<210> 267
<211> 372
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 267
Met Lys Arg Tyr Lys Lys Leu Leu Met Val Ala Pro Leu Ala Cys Met
1 5 10 15
Leu Gly Thr Gly Val Phe Thr Leu Pro Asn Val Ser His Ala Ala Glu
20 25 30
Val Ala Asp Val Tyr Asn Gln Gly Thr Thr Tyr Ser Lys Glu Asn Met
35 40 45
Glu Lys Ala Leu Tyr Leu Arg Leu Leu Glu Gly Ile Glu Arg Ser Pro
50 55 60
Glu Leu Arg Gln Lys Phe Lys Met Asn Gln Asp Glu Lys Ile Gln Arg
65 70 75 80
Glu Tyr Phe Pro Glu Asn Asp Pro Leu Val Gly Gly Ile Ala Gly Thr
85 90 95
Ile Gly Thr Met Gly Gly Ala Ile Gly Gly Ala Ala Leu Ala Ser Arg
100 105 110
Val Asp Tyr Gly Leu Lys Ser Gln Leu Asn Gln Val Asn Ile Thr Ser
115 120 125
Val Gly Thr Asp Phe Lys Val Thr Pro Ser Lys Val Val Glu Glu Asp
130 135 140
Gln Gly Val Ile Asn Leu Leu Ser Tyr Arg Asn Asn Thr Pro Glu Lys
145 150 155 160
Gln Lys Ala Phe Thr Thr Glu Glu Ser Tyr Lys Lys Val Asp Thr Val
165 170 175
Thr Ala Thr Asn Gln Tyr Gly Phe Lys Leu Ser Ala Lys Ser Val Thr
180 185 190
Lys Phe Gly Phe Asp Ile Gly Leu Ala Lys Gly Glu Gln Thr Phe Glu
195 200 205
Val Ser Ala Glu Phe Ala Phe Asn Arg Thr Asp Thr Phe Thr Thr Thr
210 215 220
Asn Glu Lys Thr Val Thr Phe Lys Ala Gln Glu Val Ile Ala Ala Ser
225 230 235 240
Gly Gly Thr Thr Asp Tyr Phe Ser Arg Val Lys Lys Ala Lys Phe Ser
245 250 255
Gly Ser Phe Gln Thr Asp Ala Ala Leu Asp Ser Leu Lys Val Lys Leu
260 265 270
Pro Ile Val Lys Gly Gly Asn Met Asn Val Thr His Thr Glu Glu Val
275 280 285
Thr Leu Asn Ser Glu Asp Leu Tyr Asn Ile Phe Lys Leu Thr Ser Lys
290 295 300
Asp Phe Pro Leu Pro Ser Tyr Leu Ser Phe Asp Asp Thr Asn Lys Ser
305 310 315 320
Ile Leu Val Lys Asn Ala Thr Phe Asn Tyr Thr Gly Glu Ile Gly Tyr
325 330 335
His Thr Ala Ile Asp Ala Lys Phe Thr Pro Phe Asp Gln Thr Lys Ala
340 345 350
Thr Ala Val Met Pro Tyr Ala Lys Tyr Ala Glu Lys Thr Gln Ala Asn
355 360 365
Ala Leu Leu Arg
370
<210> 268
<211> 343
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 268
Met Ala Glu Val Ala Asp Val Tyr Asn Gln Gly Thr Thr Tyr Ser Lys
1 5 10 15
Glu Asn Met Glu Lys Ala Leu Tyr Leu Arg Leu Leu Glu Gly Ile Glu
20 25 30
Arg Ser Pro Glu Leu Arg Gln Lys Phe Lys Met Asn Gln Asp Glu Lys
35 40 45
Ile Gln Arg Glu Tyr Phe Pro Glu Asn Asp Pro Leu Val Gly Gly Ile
50 55 60
Ala Gly Thr Ile Gly Thr Met Gly Gly Ala Ile Gly Gly Ala Ala Leu
65 70 75 80
Ala Ser Arg Val Asp Tyr Gly Leu Lys Ser Gln Leu Asn Gln Val Asn
85 90 95
Ile Thr Ser Val Gly Thr Asp Phe Lys Val Thr Pro Ser Lys Val Val
100 105 110
Glu Glu Asp Gln Gly Val Ile Asn Leu Leu Ser Tyr Arg Asn Asn Thr
115 120 125
Pro Glu Lys Gln Lys Ala Phe Thr Thr Glu Glu Ser Tyr Lys Lys Val
130 135 140
Asp Thr Val Thr Ala Thr Asn Gln Tyr Gly Phe Lys Leu Ser Ala Lys
145 150 155 160
Ser Val Thr Lys Phe Gly Phe Asp Ile Gly Leu Ala Lys Gly Glu Gln
165 170 175
Thr Phe Glu Val Ser Ala Glu Phe Ala Phe Asn Arg Thr Asp Thr Phe
180 185 190
Thr Thr Thr Asn Glu Lys Thr Val Thr Phe Lys Ala Gln Glu Val Ile
195 200 205
Ala Ala Ser Gly Gly Thr Thr Asp Tyr Phe Ser Arg Val Lys Lys Ala
210 215 220
Lys Phe Ser Gly Ser Phe Gln Thr Asp Ala Ala Leu Asp Ser Leu Lys
225 230 235 240
Val Lys Leu Pro Ile Val Lys Gly Gly Asn Met Asn Val Thr His Thr
245 250 255
Glu Glu Val Thr Leu Asn Ser Glu Asp Leu Tyr Asn Ile Phe Lys Leu
260 265 270
Thr Ser Lys Asp Phe Pro Leu Pro Ser Tyr Leu Ser Phe Asp Asp Thr
275 280 285
Asn Lys Ser Ile Leu Val Lys Asn Ala Thr Phe Asn Tyr Thr Gly Glu
290 295 300
Ile Gly Tyr His Thr Ala Ile Asp Ala Lys Phe Thr Pro Phe Asp Gln
305 310 315 320
Thr Lys Ala Thr Ala Val Met Pro Tyr Ala Lys Tyr Ala Glu Lys Thr
325 330 335
Gln Ala Asn Ala Leu Leu Arg
340
<210> 269
<211> 395
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 269
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Thr Ser Gln Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Ser Val His Leu Ala Ala Asp Thr Ala Leu Lys
35 40 45
Trp Asp Val Tyr Gly Asn Gly Thr Tyr Asp Asp Ala Gly Ile Leu Leu
50 55 60
Tyr Asn Gly Asn Leu Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Ala Val
85 90 95
Thr Ile Gly Gly Gly Trp Val Gly Asp Asn Ala Phe Ser Tyr Asn Arg
100 105 110
Glu Gly Leu Ile Gln Ser Lys Trp Thr Gly Ala Ala Asp Asn Lys Trp
115 120 125
Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly
130 135 140
Tyr Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile Thr Gly Pro Lys
145 150 155 160
Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn Asp Arg Leu Phe
165 170 175
Lys Val Ser Lys Ser Pro Trp Leu Tyr Gly Asp Ile Pro Gly Leu Asn
180 185 190
Leu Asp Tyr Gly Thr Phe Ser Leu Pro Gln Leu Pro Ala Thr Gly Asn
195 200 205
Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Ile Asp Gln Gln Leu Pro
210 215 220
Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Val Pro Cys Ile
225 230 235 240
Met Val Asn Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser
245 250 255
Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser
260 265 270
Lys Val Ile Gln Pro Gly Leu Ser Glu Ser Tyr Ser Tyr Lys Thr Gly
275 280 285
Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys
290 295 300
Phe Gly Ala Asp Leu Gly Ile Lys Phe Gly Asp Val Ser Ala Ser Leu
305 310 315 320
Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile Ser Thr Thr Asn
325 330 335
Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr Thr Ala Thr Gly Glu Pro
340 345 350
Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys Tyr Thr
355 360 365
Leu Lys Arg Ala Asp Gly Ser Thr Val Ser Asp Pro Trp Val Val Lys
370 375 380
Asn Asp Lys Ile Thr Val Thr Arg Lys Thr Ser
385 390 395
<210> 270
<211> 366
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 270
Met Pro Glu Asn Asn Arg Tyr Tyr Ser Val His Leu Ala Ala Asp Thr
1 5 10 15
Ala Leu Lys Trp Asp Val Tyr Gly Asn Gly Thr Tyr Asp Asp Ala Gly
20 25 30
Ile Leu Leu Tyr Asn Gly Asn Leu Gly Asp Asn Glu Gln Phe Val Phe
35 40 45
Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Ala Val Thr Ile Gly Gly Gly Trp Val Gly Asp Asn Ala Phe Ser
65 70 75 80
Tyr Asn Arg Glu Gly Leu Ile Gln Ser Lys Trp Thr Gly Ala Ala Asp
85 90 95
Asn Lys Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val
100 105 110
Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile Thr
115 120 125
Gly Pro Lys Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn Asp
130 135 140
Arg Leu Phe Lys Val Ser Lys Ser Pro Trp Leu Tyr Gly Asp Ile Pro
145 150 155 160
Gly Leu Asn Leu Asp Tyr Gly Thr Phe Ser Leu Pro Gln Leu Pro Ala
165 170 175
Thr Gly Asn Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Ile Asp Gln
180 185 190
Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Val
195 200 205
Pro Cys Ile Met Val Asn Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile
210 215 220
His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys
225 230 235 240
Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu Ser Tyr Ser Tyr
245 250 255
Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu
260 265 270
Ser Met Lys Phe Gly Ala Asp Leu Gly Ile Lys Phe Gly Asp Val Ser
275 280 285
Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile Ser
290 295 300
Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr Thr Ala Thr
305 310 315 320
Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr
325 330 335
Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Thr Val Ser Asp Pro Trp
340 345 350
Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys Thr Ser
355 360 365
<210> 271
<211> 377
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 271
Met Thr Asn Glu Asn Asn Thr Asn Thr Ser Ala Ser Asn Gly Glu Asn
1 5 10 15
Leu Ile Pro Ser Glu Val Leu Ala Ser Gly Lys Gln Ile Ser Phe Pro
20 25 30
Asp Phe Asn Phe Tyr Lys Ile Arg Thr Phe Cys Gly Lys Tyr Ile Asp
35 40 45
Ile Arg Gly Gly Ser Met Asp Asp Leu Ala Tyr Ala Leu Gln Tyr Thr
50 55 60
Gly His Asn Ala Asp Asn Gln Lys Phe Leu Ile Phe Thr Leu Asp Asn
65 70 75 80
Asn Tyr Ser Val Ile Ala Ala Lys His Ser Gly Lys Val Met Asp Ile
85 90 95
Trp Asp Thr Pro Asn Phe Pro Pro Gly Leu Pro Asp Ile Leu Thr Gln
100 105 110
Tyr Tyr Phe Lys Asn Gly Asp Asn Gln Lys Phe Phe Ile Ala Asn Asp
115 120 125
Gly Ser Val Ala Val Lys Gln Asp Gly Arg Val Leu Asp Val His Asp
130 135 140
Gly Arg Thr Ser Asn Asp Val Pro Ile Ile Ala Tyr Asn Tyr Gln Gly
145 150 155 160
Ser Ala Asn Gln Lys Phe Asn Leu Glu Lys Ser Gly Thr Val Ser Ile
165 170 175
Lys Pro Pro Thr Thr Gly Thr Leu Pro Pro Ala Pro Asp Phe Lys Thr
180 185 190
Thr Asn Leu Asp Glu Glu Leu Pro Asp Asp Thr Thr Pro Val Ile Thr
195 200 205
His Ala Thr Tyr Leu Pro Tyr Phe Met Val Lys Asp Pro Tyr Tyr Asn
210 215 220
Ala Gln Gln Lys Ile Thr Asn Ser Pro Tyr Tyr Ile Leu Val Arg Arg
225 230 235 240
Gln Tyr Trp Glu Lys Lys Thr Gln Arg Val Leu Ala Pro Ser Glu Thr
245 250 255
Tyr Asp Tyr Met Glu Thr Thr Gly Val Ser Arg Thr Asp Gln Thr Ser
260 265 270
Met Thr Glu Thr Thr Glu Ile Ser Ile Gly Ala Asp Leu Gly Phe Met
275 280 285
Phe Lys Gly Phe Ser Leu Gly Leu Ser Thr Ser Ile Thr Lys Ser Leu
290 295 300
Ser Val Thr Lys Ser Thr Ser Asn Thr Glu Ser Thr Glu Ile Ser Lys
305 310 315 320
Arg Val Val Tyr Thr Asn Pro Phe Lys Tyr Gln Ile Ala Tyr Ala Lys
325 330 335
Tyr Met Leu Ile Asn Glu Tyr Tyr Val Thr Arg Ser Asn Gly Glu Met
340 345 350
Ile Thr Ser Gly Asp Ala Ser Tyr Trp Lys Val Pro Asp Pro Glu Arg
355 360 365
Thr Val Ser Arg Ile Ile Pro Arg Arg
370 375
<210> 272
<211> 369
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 272
Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Ser Gly Asn Gln Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Tyr Leu Ala Asp Ala Pro Ser Glu Arg Phe Phe
35 40 45
Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Ile Ser Gly Asn Gln Thr Gly Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ala Pro Ser Glu Tyr Trp Tyr
85 90 95
Phe His Arg Glu Ala Asp Gly His Tyr Val Ile Glu Ser Lys His Ser
100 105 110
Gly Lys Val Leu Asp Ile Ala Gly Asn Ser Thr Ser Asn Asn Ala Asn
115 120 125
Val Gln Gln Tyr Glu Phe Leu Thr Asp Ala Pro Ser Glu Arg Phe Ala
130 135 140
Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile Asn Thr Gln Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp Gln Leu Pro Glu
165 170 175
Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val Pro Ala Ile Ala
180 185 190
Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Glu
210 215 220
Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr Thr Thr Lys Thr
225 230 235 240
Gly Ile Lys Val Thr Asp Gln Glu Thr Ala Thr Lys Thr Val Ser Leu
245 250 255
Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly Phe Ser Val Gly
260 265 270
Met Ser Ser Gln Tyr Ser Thr Gln Leu Gln Thr Ser Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu Ile Lys Asn Pro
290 295 300
Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu Thr Thr Glu Tyr
305 310 315 320
Ser Val Gln Arg Lys Ser Gly Thr Ile Val Asn Ser Pro Trp Thr Met
325 330 335
Thr Asp Lys Thr Arg Thr His Ala Val Thr Tyr Pro Asn Ala Glu Glu
340 345 350
Lys Ala Leu Asn Glu Asn Thr Lys Lys Leu Ser Lys Thr Glu Ser Val
355 360 365
Asn
<210> 273
<211> 627
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 273
Met Glu Glu Asn Lys Ala Asn Ile Gln Lys Ala Pro Ser Val Ser Ala
1 5 10 15
Thr Asp Gly Pro Val Phe Lys Lys Glu Lys Asp Gly Ser Phe Thr Val
20 25 30
Leu Lys Asp Lys Ile Phe Asn Met Glu Thr Leu Lys Ala Ile Gly Ser
35 40 45
Leu Gly Gly Ala Gly Leu Lys Gln Ala Tyr Ala Asp Ser Gln Val Asp
50 55 60
Gly Gly Asn Phe Asn Asn Thr Phe Arg Thr Leu Ala Met Gly Ser Ala
65 70 75 80
Ala Leu Ile Pro Tyr Gly Gly Val Val Leu Ser Pro Leu Ile Gly Leu
85 90 95
Leu Trp Ser Glu Asp Lys Thr Ala Glu Ala Asn Gln Leu Lys Lys Leu
100 105 110
Met Glu Ser Ile Ala Ala Gln Thr His Asp Gln Ile Ala Lys Tyr Asp
115 120 125
Thr Ala Lys Leu Ser Gln Ala Leu Gln Asp Leu Glu Glu Lys Val Ala
130 135 140
Glu Phe Glu Lys Ile Val Pro Ile Pro Gly Asn Gly Met Phe Tyr Ser
145 150 155 160
Asp Gly Gln Leu Gly Asp Tyr Glu Asn Ile Gln Ile Ser Ala Lys Asn
165 170 175
Leu Asn Asn Ser Phe Arg Thr Ala Ile Ser Ala Cys Gln Thr Glu Gly
180 185 190
Tyr Lys Glu Ser Glu Leu Pro Ile Tyr Met Ile Leu Ala Thr Ala His
195 200 205
Met Asn Phe Met Asn Tyr Val Ile Glu Asn Gly Glu Glu Leu Leu Lys
210 215 220
Phe Lys Pro Ser Val Leu Gln Ala Glu Phe Thr Asp Lys Gln Asn Gly
225 230 235 240
Tyr Thr Asp Gly Tyr Ile Lys Tyr Val Thr Ser Phe Ala Asn Lys Gly
245 250 255
Phe Ala Asn Lys Gly Phe Asp Met Ala Asn Gln Val Val Arg Gly Met
260 265 270
Pro Asp Leu Val Arg Lys Arg Asp Glu Val Ser Asn Glu Ile Asp Arg
275 280 285
Asn Leu Arg Thr Gln Val Met Asn Leu Ile Ile Gln Gly Thr Ser Leu
290 295 300
Thr Leu Glu Glu Thr Leu Asn Lys Ser Ile Asn Asn Ile Ala Phe Lys
305 310 315 320
Gln Ala Ala Gly Ile Lys Ser Glu Trp Thr Leu Asp Gln Asn Gly Arg
325 330 335
Pro Phe Tyr Tyr Asp Leu Lys Gly Gln Met Gln Thr Glu Trp Lys Glu
340 345 350
Ile Ser Thr Ser Phe Ala Thr Lys Tyr Glu Gly Thr Tyr Pro Leu Thr
355 360 365
His Glu Ser Leu Lys Lys Leu Leu Pro Tyr Ser Trp Tyr Tyr Phe Ser
370 375 380
Pro Lys Lys Thr Asp Thr Phe Glu Lys Gly Glu Met Tyr Ser Ser Thr
385 390 395 400
Thr Gln Thr Ile Lys Asp Lys Glu Tyr Arg Phe Asn Gln Glu Gly Lys
405 410 415
Cys Leu Asn Pro Asp Gly Gln Pro Thr Asn Asp Trp Val Gln Phe Asp
420 425 430
Asn Arg Trp Tyr Tyr Phe Ser Pro Glu Asp Ser Thr Lys Asn Thr Asp
435 440 445
Gly Val Thr Phe Lys Lys Gly Ala Met Val Thr Gly Asn Val Tyr Ile
450 455 460
Asn Asn Lys Leu Tyr Gln Phe Asn Tyr Arg Gly Glu Cys Lys Asn Pro
465 470 475 480
Asn Ser Thr Asp Ser Val Ile Ser Asp Gly Thr Tyr Lys Ile Val Tyr
485 490 495
Pro Lys Ala Asn Lys Val Val Asp Phe Gly Tyr Asp Gly Asn Glu His
500 505 510
Pro Val Ile Trp Asp Tyr His Asn Gly Lys Asn Gln Gln Trp Glu Phe
515 520 525
Lys Tyr Asp Ala Ala Lys Asn Ala Tyr Gln Ile Ile Asn Lys Asp Asp
530 535 540
Gly Arg Val Leu Ala Tyr Asn Thr Ser Gly Ala Ser Asp Thr Ala Leu
545 550 555 560
Val Thr His Asn Asp His Lys Arg Glu His Tyr Trp Thr Leu Glu Asp
565 570 575
Ala Gly Asp Gly Asn Val Leu Leu Val Asn Tyr Ala Asn Lys Asn Lys
580 585 590
Val Leu Asp Val Tyr Gly Glu Lys Thr Ala Glu Gly Ala Arg Leu Tyr
595 600 605
Val Gln Asp Lys Leu Lys Asn Ser Asn Ala Gln Lys Phe Lys Leu Val
610 615 620
Lys Val Gly
625
<210> 274
<211> 478
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 274
Met Glu Val Gln Thr Gln Thr Val Gln Glu Leu Pro Lys Ser Phe Leu
1 5 10 15
Asn Asp Leu Ser Gly Glu Phe Asn Ala Pro Pro Ser Ile Asp Ser Ser
20 25 30
Asp Val Lys Lys Leu Met Ile Asp Ala Ser Gln Asp Ser Arg Lys Ile
35 40 45
Asn Asp Leu Leu Arg Gln Ile Ser Met Gly Val Ala Gly Lys Ile Pro
50 55 60
Ala Val Gly Ser Val Val Ser Ala Met Val Gly Tyr Leu Tyr Pro Glu
65 70 75 80
Gln Asn Gly Thr Asp Ser Lys Leu Asn Ala Leu Glu Ala Lys Leu Thr
85 90 95
Ala Lys Ile Glu Lys Glu Val Asn Ala Gln Arg Val Glu Asp Ile Lys
100 105 110
Ser Arg Ile Lys Asn Leu Thr Asn Ala Ala Asn Glu Leu Gln Val Ala
115 120 125
Leu His Lys Thr Lys Ser Gly Ser Tyr Tyr Asp Gly Ser Val Gly Asp
130 135 140
Lys His Phe Thr Leu Arg Met Lys Ala Asp Ala Val Asp Thr Ile Phe
145 150 155 160
Lys Asp Leu Ile Ser Phe Leu Thr Gln Asp Gly His Glu Ile Gly Asp
165 170 175
Leu Pro Val Tyr Thr Lys Val Ala Ala Ala His Ile Ile Phe Leu Asn
180 185 190
Tyr Met Lys Thr Arg Gly Thr Asp Ser Gln Leu Tyr Arg Tyr Asp Thr
195 200 205
Pro Ser Thr Val Asp Ala His Phe Gln Pro Thr Asn Lys Val Asn Thr
210 215 220
Tyr Ala Lys His Ile Glu Glu Thr Phe Lys Lys Gly Asp Asp Lys Ile
225 230 235 240
Val Gln Leu Ile Asn Glu Leu Glu Asp Lys Lys Glu Lys Leu Arg Asn
245 250 255
Val Gly Ser Ser Thr Gly Gly Ser Leu Ala Gly Gly Asn Leu Asn Ala
260 265 270
Glu Arg Asp Arg Ser Arg Leu Asn Lys Glu Ile Asp Gly Leu Lys Ala
275 280 285
Leu Asn Leu Asn Arg Lys Arg Ser Leu Tyr Arg Asp Leu Thr Val Ala
290 295 300
Glu Gly Ser Phe Glu Ala Ala Ser Gly Lys Lys Leu Thr Tyr Thr His
305 310 315 320
Phe Asp Asp Val Leu Ser Gly Thr Tyr Lys Ile Val Ser Lys Leu Asp
325 330 335
Ser Ser Met Ile Leu Asp Asn Asp Tyr Gly His Glu Arg Leu Ala Lys
340 345 350
Leu Asn Lys Asp Tyr Thr Asp Lys Lys Glu Glu Trp Val Asn Gly Glu
355 360 365
Lys Trp Thr Leu Gln Tyr Asn Ala Asp Lys Lys Ala Tyr Lys Ile Ile
370 375 380
Asn Gln Lys Ala Pro Tyr Asp Glu Leu Ala Ile Asn Gly Asn Ser Gly
385 390 395 400
Asp Asp Thr Val Phe Ala Thr Thr Asp Arg Lys Leu Asp Ser Glu Pro
405 410 415
Thr Ala Asp Leu Arg Tyr Trp Lys Val Glu Asp Ala Gly Asn Gly Tyr
420 425 430
Phe Phe Ile Lys Asn Leu Lys Thr Gly Arg Val Leu Asp Val Thr Asp
435 440 445
Gly Lys Thr Asp Val Gly Thr Val Ile Lys Thr His Ala Gln Asn Ala
450 455 460
Ser Pro Val Ala Ala Gln Met Phe Lys Ile Glu Thr Val Gln
465 470 475
<210> 275
<211> 543
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 275
Met Lys Tyr Lys Asp Arg Lys Asp Ala Gln Arg Lys Tyr Lys Gln Ala
1 5 10 15
Ile Leu Thr Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Pro Thr Ala Ser Val Phe Ala Ala Glu Gly Asp Asn Ile Ser Val Ile
35 40 45
Asn His Val Glu Thr Gln Thr Glu Asn Ser Lys Leu Leu Glu Ser Lys
50 55 60
Trp Met Asn Lys Phe Asn Ala Glu Met Thr Asn Leu Lys Ser Val Gly
65 70 75 80
Lys Phe Thr Gly Thr Thr Met Ser Glu Ile Tyr Lys Phe Ala Asn Lys
85 90 95
Gly Pro Glu Gln Leu Asn Asp Thr Leu Arg Ala Ile Thr Ile Ala Ser
100 105 110
Ala Asp Leu Ile Pro Tyr Gly Gly Met Leu Val Ala Pro Leu Ile Ser
115 120 125
Ala Ile Trp Pro Glu Thr Asn Gly Ile Asn Ser Gln Leu Asn Ala Leu
130 135 140
Arg Glu Asp Leu Thr Lys Leu Val Thr Asn Glu Ile Asp Asn Glu Gln
145 150 155 160
Leu Asn Phe Leu Asn Ser Glu Phe Lys Thr Leu Ile Asp Leu Gln Thr
165 170 175
Asp Leu Asp His Ala Val Asn Ala Asp Asn Ser Gln Asn Thr Asp Glu
180 185 190
Asn Thr Lys Asn Gln Arg Gly Ala Trp Ala Asn Gly Ile Glu Gln Ser
195 200 205
Phe Arg Arg Leu Leu Asp His Thr Ser Ser Asp Lys His Lys Ile Thr
210 215 220
Asn Leu Pro Ile Tyr Thr Lys Val Ala Leu Ala His Leu Leu Phe Leu
225 230 235 240
Asp Ser Leu Asn Asn Lys Ala Val Ser Phe Lys Met Asn Ile Thr Glu
245 250 255
Gln Ser Leu Lys Glu Leu Tyr Thr Lys Asp Gly Val Lys Thr Leu Ala
260 265 270
Asn Asp Tyr Val Leu His Ile Met Lys Thr Phe Tyr Asp Ala Pro Gln
275 280 285
Ile Lys Lys Ile Lys Gln Val Pro Glu Phe His Phe Asn Pro Asp Asp
290 295 300
Ala Glu Asn Gln Lys Lys Asp Leu Lys Asp Arg Leu Ser Lys Val Glu
305 310 315 320
Ala Glu Asn Tyr Gln Trp Gln Met Asn Ile Ala Ser Ala Gly Gly Phe
325 330 335
Gly Ser Ala Glu Asn Lys Glu Tyr Lys Phe Lys Arg Ala Ile Leu Glu
340 345 350
Asp Ala Ile Ser Ser Tyr Glu Phe Val Glu Lys Leu Tyr Asn Thr Thr
355 360 365
Val Gly Asp Pro Met Ile Lys Glu Leu Val Lys Asp Ser Asp Asp Pro
370 375 380
Met Ile Lys Glu Leu Ala Lys Asp Ser Ser Glu Val Phe Val Ala Asp
385 390 395 400
Gly Pro Tyr Lys Ile Val Tyr Ser Lys Ala Asn Lys Val Val Asn Phe
405 410 415
Gly Ser Asp Gly Ile Glu His Pro Val Ile Gly Asp Phe His Tyr Gly
420 425 430
Lys Asn Arg Glu Arg Gln Trp Trp Gly Phe Lys Tyr Asp Ala Asp Lys
435 440 445
Lys Ala Tyr Gln Ile Ile Asn Arg Ala Asp Gly Arg Val Leu Ala Tyr
450 455 460
Asn Thr Thr Pro Asn Ala Asp Asp Thr Ala Leu Val Thr Glu Asn Gln
465 470 475 480
His Lys Pro Glu His Tyr Trp Ile Leu Glu Asp Ala Gly Ala Gly Asn
485 490 495
Val Leu Leu Val Asn Tyr Glu Asn Lys Asn Lys Val Leu Asp Val Tyr
500 505 510
Asp Glu Lys Thr Asp Asp Gly Ser Arg Val Tyr Val Arg Asp Lys Leu
515 520 525
Asn Asn Ser Asn Ala Gln Lys Phe Lys Leu Glu Phe Leu Asn Ile
530 535 540
<210> 276
<211> 360
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 276
Met Ala Thr Pro Glu Lys Asn Gly Tyr Tyr Ser Ile His Leu Ala Ser
1 5 10 15
Asn Thr Trp Lys Lys Trp Asp Val Thr Arg Gly Ser Leu Asp Asn Glu
20 25 30
Val Gly Ile Leu Leu Tyr Asp Val Ser Gly Gln Ile Pro Asp Asn Gln
35 40 45
Gln Phe Ala Phe Phe Pro Leu Asp Asn Gly Lys Tyr Ala Ile Val Asn
50 55 60
Lys Asn Ser Gly Lys Pro Val Thr Ile Gly Asp Trp Val Gln Pro Met
65 70 75 80
Ala Met Leu Thr Gly Tyr Asn His Asp Thr Leu Lys Gln Ser Asn Trp
85 90 95
Thr Gly Asn Ala Asn Glu Gln Trp Tyr Leu Arg Asp Gln Gly Ser Asn
100 105 110
Asn Tyr Glu Ile Val Asn Gln Gly Asn Gly Lys Val Ala Ser Tyr Ala
115 120 125
Trp Thr Gly Asp Leu Ala Arg Asn Tyr Glu Tyr Val Asp Leu Asp Asn
130 135 140
Ser Asn Pro Ser Asp Lys Asp Arg Val Phe Thr Ile Pro Ala Ala Arg
145 150 155 160
Ser Thr Phe Thr Leu Pro Thr Leu Pro Ala Thr Gly Thr Arg Ser Glu
165 170 175
Ala Pro Asn Tyr Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser
180 185 190
Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Lys
195 200 205
Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr
210 215 220
Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile
225 230 235 240
Gln Pro Gly Leu Ser Glu Ser Tyr Ser Tyr Lys Thr Gly Val Ser Ser
245 250 255
Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ala
260 265 270
Asp Phe Gly Ile Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu
275 280 285
Ile Ser Arg Thr Ile Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala
290 295 300
Thr Glu Glu Thr Arg Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala
305 310 315 320
Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg
325 330 335
Ala Asp Gly Ser Val Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys
340 345 350
Ile Thr Val Thr Arg Lys Thr Ser
355 360
<210> 277
<211> 525
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 277
Met Ala Glu Asn Thr Gly Gln Thr Thr Thr Gln Val Lys Glu Gly Phe
1 5 10 15
Glu Arg Val Glu Pro Leu Ile Gln Pro Lys Ile Gln Tyr Ala Asn Leu
20 25 30
Asp Pro Lys Lys Ile Gly Glu Ala Val Thr Gly Ala Phe Asp Asn Phe
35 40 45
Thr Ser Phe Ile Leu Ser Lys Gln Lys Val Ala Lys Leu Asp Glu Asn
50 55 60
Gly Val Pro Val Arg Asp Glu Leu Gly Lys Val Val Gln Val Ser Leu
65 70 75 80
Asp Glu Lys Glu Ile Lys Lys Ile Lys Asp Ser Leu Asn Lys Met Ser
85 90 95
Lys Pro Gly Ala Ala Val Leu Lys Lys Ala Ala Glu Gly Gly Asp Gly
100 105 110
Gln Glu Ile Leu Val Ala Tyr Val Glu Ser Leu Leu Ser Met Ala Pro
115 120 125
Tyr Gly Ala Phe Ile Ser Pro Leu Leu Ser Met Leu Leu Pro Glu Lys
130 135 140
Lys Gly Glu Asn Ile Met Asp Gln Val Asn Lys Ala Ile Asp Lys Lys
145 150 155 160
Leu Lys Ser Gly Ile Glu Asn Tyr Asp Ile Ala Gly Leu Asn Ala Ala
165 170 175
Phe Glu Asn Val Glu Asp Ser Val Gln Thr Ala Glu Asn Tyr Leu Ala
180 185 190
Lys Asp Leu Lys Gly Asp Glu Arg Arg His Leu Thr Asp Ser Met Lys
195 200 205
Ala Lys Asn Lys Ala Leu Lys Asn Phe Leu Thr Val Ala Ser Lys Lys
210 215 220
Gly Tyr Glu Val Ala Glu Leu Pro Ile Tyr Gly Val Ala Ala Ile Ala
225 230 235 240
His Leu Gly Phe Leu Lys Ser Met Ser Tyr Glu Ser Ala Arg Lys Ile
245 250 255
Ala Glu Ile Pro Asp Ser Val Tyr Asp Ala Glu Phe Thr Lys Phe Lys
260 265 270
Asp Ala Ala Lys Thr Tyr Gly Asp His Ile Asn Asp Thr Tyr Lys Met
275 280 285
Lys Leu Val Glu Phe Glu Lys Asn Met Lys Glu Ala Ile Glu Asp Gly
290 295 300
Thr Asn Val Ser Ser Lys Glu Val Thr Ile Asp Asn Tyr Lys Asp Val
305 310 315 320
Leu Asn Lys Ala Tyr Asp Glu Ala Lys Asn His Arg Thr Thr Thr Ser
325 330 335
His Gly Ser Tyr Ser Ser Pro Glu Glu Lys Arg Met Asn Ala Ile Thr
340 345 350
Thr Ala Ile Gln Glu Cys Asp Ala Leu Ile Asn Glu Leu Asn Lys Tyr
355 360 365
Lys Asn Leu Thr Val Asn Asn Val Ala Leu Asn Lys Leu Ile Tyr Asn
370 375 380
Gln Asp Thr Asn Pro Asn Val Ala Val Ser Lys Trp Ser Gln Leu Asp
385 390 395 400
Asp Gly Lys Trp Tyr His Phe Asn Ser Val Gly Gln Lys Asp Thr Gly
405 410 415
Trp Phe Gln Asp Asn Asp Lys Ser Trp Tyr Tyr Leu Gly Ser Asp Gly
420 425 430
Ala Met Val Thr Gly Trp Tyr Lys Tyr Gly Ser Ser His Phe Tyr Leu
435 440 445
Asn Asp Thr Leu Lys Thr Asn Tyr Lys Gly Glu Thr Val Lys Lys Gly
450 455 460
Gln Met Ile Thr Gly Trp Tyr Tyr Asn Thr Asp Asn Lys Tyr Tyr Tyr
465 470 475 480
Leu Lys Pro Asp Asn Gly Ala Met Val Gln Asn Asp Thr Ile Lys Ile
485 490 495
Asp Gly Ala Asp Ala Arg Phe Asp Glu Asn Gly Val Trp Gly Gly Gly
500 505 510
Lys Val Glu Lys Ser Ser Asn Asp Lys Ser Thr Arg Glu
515 520 525
<210> 278
<211> 383
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 278
Met Lys Lys Ala Cys Leu Ile Gly Leu Val Ala Thr Thr Ile Ser Thr
1 5 10 15
Ser Cys Leu Ser Thr Val Thr Thr Leu Ala Ala Pro Leu Ser Glu Ser
20 25 30
Gln Gln Val Gln Gly Val Ala Ala Val Asn Thr Leu Ser Asn Ser Ile
35 40 45
Lys Met Leu Gly Ile Gln Ala Pro Leu Ile Gln Ala Tyr Gly Leu Val
50 55 60
Ile Leu Gln Gln Pro Asp Ala Arg Val Glu Ala Met Ser Ser Leu Thr
65 70 75 80
Asn His Gln Ala Phe Ala Lys Thr Asn Val Arg Glu Trp Leu Asp Glu
85 90 95
Tyr Asn Pro Gln Ile Leu Ala Met Asn Gln Glu Met Ile Arg Cys Asn
100 105 110
Asn Arg Phe Asn Gly Tyr Tyr Asn Lys Leu Leu Asp Leu Leu Gly Ser
115 120 125
Leu Asn Asn Asn Glu Gln Ala Lys Lys Asp Phe Ile Ile Ala Val Asp
130 135 140
Gln Met Gln Gln Gln Ala Gln Ala Ala Phe Glu His Leu Glu Gln Thr
145 150 155 160
Ser Ser Ser Leu Asn Arg Phe Lys Val Gln Leu Val Thr Asp Ser Lys
165 170 175
Ser Leu Ser Glu Lys Ser Asn Ile Ala Ile Gln Ser Leu Gln Gly Thr
180 185 190
Asn Gly Asn Ile Glu Gln Leu Arg Ala Glu Val Lys Arg Leu Gln Glu
195 200 205
Glu Ile Gln Asn Asn Leu Ile Thr Ile Leu Asn Arg Pro Asp Glu Ile
210 215 220
Ile Lys Gly Ser Ile Asn Ile Gly Lys Glu Leu Phe Lys Ile Thr Ser
225 230 235 240
Ala Thr Ala Glu Ser Lys Thr Ile Asn Phe Thr Ser Ile Cys Ser Leu
245 250 255
Ser Glu Glu Phe Met Lys Ala Thr Asp Ser Lys Thr Gln Gln Ala Thr
260 265 270
Leu Asn Leu Gln Gln Lys Gln Lys Glu Leu Leu Thr Val Ile Gln Lys
275 280 285
Leu Ser Gln Thr Gln Val Gln Ala Thr Glu Ile Thr Phe Ile Glu Asp
290 295 300
Gln Val Asn Ser Phe Ala Glu Leu Ile Ser Arg Gln Val Ala Thr Phe
305 310 315 320
Asp Thr Val Ile Gly Asp Trp Lys Leu Phe Glu Glu Gly Leu Thr Gln
325 330 335
Leu Lys Ala Asn Ser His Val Gln Gly Asn Leu Asn Thr Thr Asp Leu
340 345 350
Gln Lys Gln Leu Ile Gln Leu Lys Glu Trp Asn Asp Met Leu Asn Lys
355 360 365
Gln Thr Lys Gln Phe Glu Asp Ser Val Met Gln Val Thr Val Gln
370 375 380
<210> 279
<211> 358
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 279
Met Ala Pro Leu Ser Glu Ser Gln Gln Val Gln Gly Val Ala Ala Val
1 5 10 15
Asn Thr Leu Ser Asn Ser Ile Lys Met Leu Gly Ile Gln Ala Pro Leu
20 25 30
Ile Gln Ala Tyr Gly Leu Val Ile Leu Gln Gln Pro Asp Ala Arg Val
35 40 45
Glu Ala Met Ser Ser Leu Thr Asn His Gln Ala Phe Ala Lys Thr Asn
50 55 60
Val Arg Glu Trp Leu Asp Glu Tyr Asn Pro Gln Ile Leu Ala Met Asn
65 70 75 80
Gln Glu Met Ile Arg Cys Asn Asn Arg Phe Asn Gly Tyr Tyr Asn Lys
85 90 95
Leu Leu Asp Leu Leu Gly Ser Leu Asn Asn Asn Glu Gln Ala Lys Lys
100 105 110
Asp Phe Ile Ile Ala Val Asp Gln Met Gln Gln Gln Ala Gln Ala Ala
115 120 125
Phe Glu His Leu Glu Gln Thr Ser Ser Ser Leu Asn Arg Phe Lys Val
130 135 140
Gln Leu Val Thr Asp Ser Lys Ser Leu Ser Glu Lys Ser Asn Ile Ala
145 150 155 160
Ile Gln Ser Leu Gln Gly Thr Asn Gly Asn Ile Glu Gln Leu Arg Ala
165 170 175
Glu Val Lys Arg Leu Gln Glu Glu Ile Gln Asn Asn Leu Ile Thr Ile
180 185 190
Leu Asn Arg Pro Asp Glu Ile Ile Lys Gly Ser Ile Asn Ile Gly Lys
195 200 205
Glu Leu Phe Lys Ile Thr Ser Ala Thr Ala Glu Ser Lys Thr Ile Asn
210 215 220
Phe Thr Ser Ile Cys Ser Leu Ser Glu Glu Phe Met Lys Ala Thr Asp
225 230 235 240
Ser Lys Thr Gln Gln Ala Thr Leu Asn Leu Gln Gln Lys Gln Lys Glu
245 250 255
Leu Leu Thr Val Ile Gln Lys Leu Ser Gln Thr Gln Val Gln Ala Thr
260 265 270
Glu Ile Thr Phe Ile Glu Asp Gln Val Asn Ser Phe Ala Glu Leu Ile
275 280 285
Ser Arg Gln Val Ala Thr Phe Asp Thr Val Ile Gly Asp Trp Lys Leu
290 295 300
Phe Glu Glu Gly Leu Thr Gln Leu Lys Ala Asn Ser His Val Gln Gly
305 310 315 320
Asn Leu Asn Thr Thr Asp Leu Gln Lys Gln Leu Ile Gln Leu Lys Glu
325 330 335
Trp Asn Asp Met Leu Asn Lys Gln Thr Lys Gln Phe Glu Asp Ser Val
340 345 350
Met Gln Val Thr Val Gln
355
<210> 280
<211> 390
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 280
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Gly Val Gly Ala
1 5 10 15
Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala
20 25 30
Asn Asn Glu Tyr His Thr Ile His Leu Ala Ala Asn Thr Asn Ile Ala
35 40 45
Trp Asp Val Tyr Lys Gly Trp Gln Ser Asp Asp Ala Gly Ile Val Leu
50 55 60
Trp Asp Gly Ala Arg Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Gly Ser Ala Ile Ile Gly Ser Asp Gly Val Arg Asn Phe Pro
100 105 110
Ser Phe Asn Asn Glu Gly Leu Arg Gln Thr Ser Trp Thr Gly Ala Pro
115 120 125
Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Phe Glu Ile
130 135 140
Val Asn Gln Gly Ser Gly Lys Val Ala Ser Trp Ala Gly Val Glu Met
145 150 155 160
Tyr Gly Tyr Lys Asp Tyr Val Asp Leu Asp Glu Ser Asn Pro Ser Asp
165 170 175
Asn Asp Arg Leu Phe Asn Ile Pro Ala Ala Arg Ser Thr Phe Ser Leu
180 185 190
Pro Thr Leu Pro Thr Val Gly Thr Arg Pro Asn Ala Pro Asp Tyr Asn
195 200 205
Thr Ser Gly Ala Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val
210 215 220
Val Val Gly Ala Ser Leu Ile Pro Ser Ile Met Val Lys Asp Asn Gly
225 230 235 240
Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Val Leu Val
245 250 255
Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu Ile Ile Pro Ala Gly
260 265 270
Ala Thr Ser Lys Tyr Thr Val Lys Ser Gly Val Ser Glu Ile Asp Gln
275 280 285
Lys Lys Met Ser Asp Thr Leu Ala Met Ser Phe Gly Ser Asp Leu Gly
290 295 300
Leu Lys Phe Gly Glu Ser Ser Leu Ser Ile Lys Ser Ser Val Ser Arg
305 310 315 320
Thr Ile Gln Thr Glu Val Thr Lys Thr Ser Thr Asn Ala Thr Glu Glu
325 330 335
Thr Glu Glu Lys Ser Ile Pro Ser Ile Ser Gly Lys Asp Thr Gly Phe
340 345 350
Thr Ala Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly
355 360 365
Ser Val Val Thr Asp Pro Ser Ile Val Arg Asp Ser Lys Gln Thr Ile
370 375 380
Ala Arg Thr Ile Ala Lys
385 390
<210> 281
<211> 356
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 281
Met Ala Pro Ala Lys Asn Ala Tyr His Thr Ile Gln Leu Ala Ala Asn
1 5 10 15
Ala Trp Leu Lys Trp Asp Val Tyr Gly Asn Gly Thr Tyr Asp Asp Ala
20 25 30
Gly Ile Leu Leu Tyr Asn Gly Ser Phe Gly Asp Asn Glu Gln Phe Val
35 40 45
Phe Phe Pro Leu Asp Gly Gly Ser Tyr Ala Ile Val Asn Lys Asn Ser
50 55 60
Gly Lys Ala Val Thr Thr Gly Ser Gly Trp Ile Ala Gly Asn Thr Met
65 70 75 80
Ser Tyr Asn Pro Glu Gly Leu Lys Gln Ser Lys Trp Thr Gly Ala Ala
85 90 95
Ala Asn Lys Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile
100 105 110
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Arg Ile Gly Ile
115 120 125
Ala Gly Pro Gln Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn
130 135 140
Asp Arg Leu Phe His Ile Pro Ala Ala Ala Ser Ser Thr Phe Ser Leu
145 150 155 160
Pro Thr Leu Pro Asn Val Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr
165 170 175
Ala Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val
180 185 190
Gly Ala Ser Leu Val Pro Cys Ile Met Val Asn Asp Asn Gln Ala Ser
195 200 205
Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu
210 215 220
Glu Tyr Trp Asp Lys Thr Phe Ser Ala Val Val Pro Ala Gly Leu Asn
225 230 235 240
Leu Lys Tyr Ser Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln Lys
245 250 255
Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys
260 265 270
Phe Gly Asp Ala Ser Ala Ala Ile Lys Ser Glu Ile Ser Arg Thr Ile
275 280 285
Gln Thr Glu Ile Ser Thr Ser Asn Thr Glu Val Ser Glu Glu Thr Ile
290 295 300
Met Arg Glu Ala Ser Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr Glu
305 310 315 320
Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile
325 330 335
Val Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile Ala Arg
340 345 350
Lys Asn Ala Gln
355
<210> 282
<211> 387
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 282
Met Lys Val Leu Ser Lys Lys Thr Leu Ala Gly Leu Met Ala Gly Ala
1 5 10 15
Leu Ser Leu Ser Ile Trp Ala Pro Ala Ser Glu Ala Ala Thr Pro Lys
20 25 30
Lys Asn Gln Tyr Tyr Ser Ile Lys Leu Lys Ala Asn Gln Asn Leu Ala
35 40 45
Trp Asp Val Tyr Gly Gly Arg Ile Asp Pro Leu Thr Arg Val Thr Leu
50 55 60
Tyr Asp Ala His Asn Asn Glu Asn Gln Lys Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Lys Tyr Ala Ile Val Asn Lys Asn Ser Gly Ile Pro Val
85 90 95
Ser Gly Asp Leu Ser Lys His Trp His Trp Phe Ser Ser Gln Thr Leu
100 105 110
Gly Gln Ile Gly Trp Thr Gly Ala Pro Asp Gln Gln Trp Tyr Leu Arg
115 120 125
Asp Gln Gly Ser Asn Tyr Tyr Glu Ile Val Asn Gln Glu Ser Gly Glu
130 135 140
Val Ala Ser Tyr Gly Thr His Asp Glu Asn Asn Gly Ser Ile Ala Gly
145 150 155 160
Ala Phe Ser Tyr Arg Leu Gly Leu Gly Tyr Ser Asn Pro Ser Asn Ser
165 170 175
Asn Gly Val Phe Lys Leu Thr Ala Thr Asn Asn Ile Ser Leu Pro Lys
180 185 190
Leu Pro Ser Val Gly Thr Arg Pro Ser Ala Pro Lys Tyr Thr Gly Asp
195 200 205
Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Phe Val Val Val Gly Ala
210 215 220
Ser Leu Val Pro Ser Ile Met Val Asn Asp Asn Gln Val Ser Asp Tyr
225 230 235 240
Thr Lys Ile His Asn Ser Pro Tyr Tyr Val Leu Val Lys Glu Glu Tyr
245 250 255
Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg Gln
260 265 270
Tyr Ser Phe Lys Thr Gly Met Thr Ser Thr Asp Gln Gln Lys Met Thr
275 280 285
Glu Thr Leu Ser Met Ser Ile Gly Ala Asp Leu Gly Leu Lys Phe Gly
290 295 300
Glu Ser Thr Ala Ser Ile Lys Ser Asn Ile Thr Lys Thr Leu Gln Thr
305 310 315 320
Glu Ile Ser Thr Thr Asn Thr Gln Ala Gln Glu Glu Thr Ile Thr Ala
325 330 335
Thr Ala Glu Ser Lys Ser Gly Lys Thr Thr Ala Phe Thr Glu Tyr Gln
340 345 350
Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val Ser
355 360 365
Asn Pro Trp Val Val Lys Asn Asn Lys Val Thr Ile Ala Arg Asp Asn
370 375 380
Val Gln Glu
385
<210> 283
<211> 406
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 283
Met Lys Ser Leu Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Thr Ser Glu Ala Ala Ala Pro Glu
20 25 30
Lys Asn Arg Tyr Tyr Ser Leu His Leu Thr Asn Asn Ile Glu Lys Val
35 40 45
Trp Asp Val Tyr Asn Gly Trp Thr Ser Asn Asp Ala Gly Ile Leu Leu
50 55 60
Tyr Lys Ala Asn Gly Gln Asp Asn Gln Gln Phe Val Phe Phe Gln Leu
65 70 75 80
Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Leu Gly Gly Gly Ile Asn Tyr Thr Asp Gly Val Arg Asn Met Ser
100 105 110
Asn Ile Ala Gly Asn Pro Ala Asp Thr Leu Gly Gln Asn Asp Trp Thr
115 120 125
Gly Ala Asp Thr Asp Lys Trp Tyr Leu Arg Asp Lys Gly Asn Gly Asn
130 135 140
Tyr Glu Ile Val Asn Gln Gly Asn Gly Arg Val Ala Ser Tyr Ala Ala
145 150 155 160
Thr Asn Ile Ala Pro Tyr Leu His Tyr Val Asp Leu Asp Asp Ser Asn
165 170 175
Pro Ser Asp Gly Asp Arg Leu Phe Lys Val Ser Lys Ser Pro Phe Ala
180 185 190
Phe Gly Ile Asp Ile Pro Gly Phe Asn Phe Asp Tyr Gly Thr Phe Ser
195 200 205
Leu Pro Gln Leu Pro Ala Thr Gly Asn Arg Pro Asp Ala Pro Asn Tyr
210 215 220
Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val
225 230 235 240
Val Gly Ala Ser Leu Ile Pro Cys Phe Met Val Lys Asp Ser Gln Ala
245 250 255
Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Ile Lys
260 265 270
Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu
275 280 285
Thr Arg Asn Tyr Thr Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln
290 295 300
Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu
305 310 315 320
Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr
325 330 335
Ile Gln Thr Glu Ile Thr Ser Thr Asn Thr Glu Ala Ser Glu Glu Thr
340 345 350
Ile Ser Ser Thr Val Thr Ser Glu Pro Gly Lys Ser Gln Thr Gly Phe
355 360 365
Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly
370 375 380
Thr Ile Val Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile
385 390 395 400
Ala Arg Lys Asn Ala Gln
405
<210> 284
<211> 378
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 284
Met Ala Pro Glu Lys Asn Arg Tyr Tyr Ser Leu His Leu Thr Asn Asn
1 5 10 15
Ile Glu Lys Val Trp Asp Val Tyr Asn Gly Trp Thr Ser Asn Asp Ala
20 25 30
Gly Ile Leu Leu Tyr Lys Ala Asn Gly Gln Asp Asn Gln Gln Phe Val
35 40 45
Phe Phe Gln Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser
50 55 60
Gly Lys Pro Val Gly Leu Gly Gly Gly Ile Asn Tyr Thr Asp Gly Val
65 70 75 80
Arg Asn Met Ser Asn Ile Ala Gly Asn Pro Ala Asp Thr Leu Gly Gln
85 90 95
Asn Asp Trp Thr Gly Ala Asp Thr Asp Lys Trp Tyr Leu Arg Asp Lys
100 105 110
Gly Asn Gly Asn Tyr Glu Ile Val Asn Gln Gly Asn Gly Arg Val Ala
115 120 125
Ser Tyr Ala Ala Thr Asn Ile Ala Pro Tyr Leu His Tyr Val Asp Leu
130 135 140
Asp Asp Ser Asn Pro Ser Asp Gly Asp Arg Leu Phe Lys Val Ser Lys
145 150 155 160
Ser Pro Phe Ala Phe Gly Ile Asp Ile Pro Gly Phe Asn Phe Asp Tyr
165 170 175
Gly Thr Phe Ser Leu Pro Gln Leu Pro Ala Thr Gly Asn Arg Pro Asp
180 185 190
Ala Pro Asn Tyr Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser
195 200 205
Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro Cys Phe Met Val Lys
210 215 220
Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr
225 230 235 240
Thr Leu Ile Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile
245 250 255
Pro Ala Gly Leu Thr Arg Asn Tyr Thr Phe Lys Thr Gly Met Thr Ser
260 265 270
Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala
275 280 285
Asp Phe Gly Leu Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Thr Glu
290 295 300
Ile Ser Arg Thr Ile Gln Thr Glu Ile Thr Ser Thr Asn Thr Glu Ala
305 310 315 320
Ser Glu Glu Thr Ile Ser Ser Thr Val Thr Ser Glu Pro Gly Lys Ser
325 330 335
Gln Thr Gly Phe Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys
340 345 350
Arg Ala Asp Gly Thr Ile Val Ser Asp Pro Trp Val Val Lys Asn Asn
355 360 365
Lys Ile Thr Ile Ala Arg Lys Asn Ala Gln
370 375
<210> 285
<211> 475
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 285
Met Ala Glu Gln Pro Val Gln Glu Leu Pro Lys Leu Leu Asn Asp Leu
1 5 10 15
Ser Gly Glu Phe Asn Ser Pro Leu Ile Lys Ser Glu Glu Val Ser Lys
20 25 30
Ile Met Ser Asp Ala Ser Lys Asp Ala Arg Arg Leu Asn Asp Leu Leu
35 40 45
Arg Gln Ile Ser Met Gly Val Ala Gly Lys Ile Pro Ala Val Gly Ser
50 55 60
Val Val Ser Ala Met Val Gly Tyr Leu Tyr Pro Glu Gln Asp Gly Thr
65 70 75 80
Asp Ser Lys Leu Asn Ala Leu Glu Ala Lys Leu Thr Ala Lys Ile Glu
85 90 95
Gln Ala Val Asn Asp Gln His Val Lys Asp Ile Lys Ser His Ile Gln
100 105 110
Asn Leu Thr Asn Ala Ala Asn Glu Leu Gln Thr Ala Leu Asn Ser Val
115 120 125
Asn Lys Gly Ala Phe Tyr Asp Gly Gly Ser Ile Lys Asp Ile His Gly
130 135 140
Thr Leu Arg Glu Lys Ala Glu Thr Val Asp Lys Lys Phe Lys Glu Leu
145 150 155 160
Met Pro Phe Leu Met Gln Glu Gly His Glu Ile Gly Asp Leu Pro Val
165 170 175
Tyr Thr Lys Val Ala Ala Ala His Ile Leu Phe Leu Asn Tyr Met Lys
180 185 190
Thr Arg Gly Thr Asp Ser Lys Leu Tyr Arg Tyr Asp Ser Asp Lys Thr
195 200 205
Val Asp Ala Gln Phe Gln Pro Thr Asp Arg Val Asp Thr Tyr Ala Lys
210 215 220
His Ile Glu Asp Thr Phe Lys Lys Gly Asp Asp Lys Ile Val Gln Leu
225 230 235 240
Ile Lys Glu Leu Glu Asp Lys Lys Glu Ala Leu Arg Asn Val Gly Ser
245 250 255
Ser Thr Gly Gly Ser Leu Ala Gly Gly Asn Leu Gly Ala Glu Asn Lys
260 265 270
Arg Ser Ile Leu Asn Lys Glu Ile Asp Gly Leu Lys Ala Leu Asn Leu
275 280 285
Asn Arg Lys Arg Ser Leu Tyr Arg Asp Leu Thr Val Ala Glu Gly Ser
290 295 300
Phe Glu Ala Ala Ser Gly Lys Lys Leu Thr Tyr Thr His Phe Ala Asp
305 310 315 320
Ile Leu Ser Gly Thr Tyr Lys Ile Val Ser Lys Leu Asp Ser Ser Met
325 330 335
Ile Leu Asp Asn Asp Tyr Gly His Glu Arg Leu Ala Lys Leu Asn Lys
340 345 350
Asp Tyr Thr Asp Lys Lys Glu Glu Trp Val Asn Gly Glu Lys Trp Thr
355 360 365
Phe Gln Tyr Asn Ala Asp Lys Lys Ala Tyr Lys Ile Ile Asn Gln Lys
370 375 380
Ala Pro Tyr Asp Glu Leu Ala Ile Asn Gly Lys Phe Gly Asp Asp Thr
385 390 395 400
Val Phe Ala Thr Thr Asp Arg Lys Leu Asp Ser Asp Ser Thr Ala Asp
405 410 415
Leu Arg Tyr Trp Lys Val Glu Asp Ala Gly Asn Gly Tyr Phe Phe Ile
420 425 430
Lys Asn Leu Lys Thr Gly Arg Val Leu Asp Val Thr Asp Gly Lys Thr
435 440 445
Gly Val Gly Thr Val Ile Lys Thr His Ala Gln Asn Ala Ser Pro Val
450 455 460
Ala Ala Gln Met Phe Lys Ile Glu Thr Val Gln
465 470 475
<210> 286
<211> 552
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 286
Met Asp Glu Thr Ser Thr Lys Thr Arg Gln Thr Ala Pro Glu Ser Leu
1 5 10 15
Gly Ala Asn Gly Ser Gly Ala Gly Ala Thr Thr Ser Lys Leu Glu Glu
20 25 30
Leu Leu Thr Met Asp Asn Leu Lys Lys Val Gly Thr Leu Ser Gly Asp
35 40 45
Val Leu Lys Val Ala Tyr Lys Asp Ala His Glu Asn Asn Gly Asn Tyr
50 55 60
Asn Asn Thr Phe Arg Thr Leu Ala Met Ser Gly Thr Ala Leu Ile Pro
65 70 75 80
Tyr Gly Gly Val Ile Ile Ser Pro Leu Ile Gly Leu Leu Trp Pro Glu
85 90 95
Asn Val Glu Ala Gln Lys Asn Gln Met Lys Lys Leu Ile Asp Glu Leu
100 105 110
Asn Asn Met Met Asp Gln Lys Ile Thr Asp Asn Glu Ile Ala Thr Ile
115 120 125
Glu Ser Glu Val Asn Ile Leu Lys Glu Met Leu Gln Lys Phe Glu Lys
130 135 140
Asn Ile Asn Met Asn Ile Ser Ala Gly Phe Phe Tyr Ser Pro Thr Thr
145 150 155 160
Lys Glu Gln Ala Leu Ala Gln Asp Ala Lys Ala Ile Asn Asn Lys Phe
165 170 175
Ser Asp Ile Ile Gly His Cys Lys Gln Lys Leu Gln Val Ile Pro Ala
180 185 190
Leu Pro Ile Phe Thr Ala Met Ala Thr Ala His Leu Glu Phe Leu Tyr
195 200 205
Phe Leu Glu Lys Asn Gly Thr Gly Pro Lys Leu Gln Tyr Thr Thr Asp
210 215 220
Ala Leu Asn Thr Leu Leu Gln Gly Glu Asp Asn Glu Gly Phe Gly Gln
225 230 235 240
Arg Ile Asn Ser Leu Ala Glu Thr Tyr Val Asn His Val Gln Lys Thr
245 250 255
Tyr Gln Leu Gly Lys Gln Gln Phe Asn Lys Lys Met Asp Asp Thr Ala
260 265 270
Lys Asn Thr Pro Gly Gly Asn Thr Asn Ser Thr Ser Glu Gln Glu Thr
275 280 285
Met Ser Lys Met Gln Lys Tyr Ile Phe Asp Leu His Pro Val Ala Asn
290 295 300
Asn Ala Asp Glu Lys Arg Lys Gln Met Gln Val Ala Leu Asn Asn Tyr
305 310 315 320
Val Lys Leu Arg Asn Asp Arg Asp Gln Tyr Tyr Lys Asn Thr Ala Asn
325 330 335
Asn Glu Ala Phe Gln Leu Leu Ala Ile Gly Lys Trp Lys Arg Glu Asp
340 345 350
Asn Asn Trp Tyr Phe Thr Asp Thr Lys Gly Glu Lys Lys Thr Gly Trp
355 360 365
Ile Gln Leu Gly Glu Lys His Tyr Tyr Leu Asn Pro Lys Asn Gly Ile
370 375 380
Met Val Lys Gly Phe Val Thr Ile Asp Gly Lys Pro Tyr Gln Phe Asn
385 390 395 400
Asp Ser Gly Glu Leu Ile Phe Ser Glu Gly Thr Tyr Lys Ile Val Tyr
405 410 415
Ser Lys Ala Asn Lys Val Val Asp Phe Gly Ala Tyr Gly Glu Gln Pro
420 425 430
Val Ile Trp Asp Tyr His Pro Asp Trp Asn Thr Pro Asn Thr Asn Gln
435 440 445
Gln Trp Glu Phe Lys Tyr Asp Ala Ser Asn Asn Ala Tyr Gln Ile Ile
450 455 460
Asn Lys Gln Asn Lys Lys Val Leu Ala Tyr Asn Thr Thr Ser Gly Thr
465 470 475 480
Asp Tyr Thr Ala Leu Val Thr Glu Asn Gln His Lys Ala Glu His Tyr
485 490 495
Trp Thr Leu Glu Asp Ala Gly Asp Gly Asn Ile Leu Leu Val Asn Phe
500 505 510
Ala Asn Lys Asn Lys Val Leu Asp Val Tyr Gly Glu Glu Thr Ala Asn
515 520 525
Gly Ser Lys Val Tyr Val Arg Asp Lys Leu Asn Asn Ser Met Ala Gln
530 535 540
Lys Phe Lys Leu Ile Lys Leu Asp
545 550
<210> 287
<211> 393
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 287
Met Lys Ser Met Ser Lys Lys Ile Met Thr Gly Leu Ala Ala Gly Ala
1 5 10 15
Met Gly Leu Ser Ile Trp Ala Pro Ser Ser Leu Ala Ala Thr Pro Glu
20 25 30
Lys Asn Glu Tyr His Thr Ile His Leu Ala Ala Asn Ser Asn Ile Val
35 40 45
Trp Asp Val Phe Lys Gly Trp Thr Thr Asp Asp Ala Gly Ile Ile Leu
50 55 60
Trp Asn Gly Asp Arg Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Asn Asn Ser Tyr Ala Tyr Tyr Trp Thr Asp Gly Val Arg Asn
100 105 110
Ala Gln Val Ile Asn Asp Gln Ala Leu Lys Gln Thr Asn Trp Thr Gly
115 120 125
Ala Pro Ala Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Phe
130 135 140
Glu Ile Val Asn Gln Gly Ser Gly Lys Val Ala Ser Trp Ala Gly Gln
145 150 155 160
Ser Ile Thr Gly Gly Tyr Leu Asp Tyr Val Asp Leu Asp Glu Ser Asn
165 170 175
Pro Ser Asp Asn Asn Lys Leu Phe Asn Ile Pro Ala Ala Arg Ser Thr
180 185 190
Phe Ser Leu Pro Thr Leu Pro Ala Val Gly Ser Arg Pro Asn Ala Pro
195 200 205
Glu Tyr Asn Leu Thr Gly Pro Ile Asp Gln Gln Leu Ala Gln Thr Ser
210 215 220
Asn Ser Ala Val Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Lys
225 230 235 240
Asp Ser Gly Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr
245 250 255
Val Leu Gln Lys Glu Glu Tyr Trp Glu Lys Thr Arg Ser Ala Thr Ile
260 265 270
Ser Pro Gly Gly Thr Asp Lys Tyr Phe Val Lys Thr Gly Val Ser Thr
275 280 285
Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Asn Phe Gly Val
290 295 300
Asp Leu Gly Leu Lys Phe Gly Asp Gln Ser Ala Ser Ile Lys Tyr Gly
305 310 315 320
Val Ser Lys Thr Leu Gln Thr Glu Val Ser Lys Thr Thr Thr Asn Ala
325 330 335
Thr Glu Glu Thr Glu Glu Lys Ser Ile Pro Ser Ile Asp Gly Lys Glu
340 345 350
Thr Gly Tyr Thr Val Tyr Gln Leu Val Thr Lys Tyr Thr Leu Lys Arg
355 360 365
Thr Asp Gly Ser Thr Val Ser Asp Pro Trp Thr Val Arg Asp Lys Asn
370 375 380
Gln Ile Val Val Arg Ser Ile Ala Lys
385 390
<210> 288
<211> 430
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 288
Met Ile Leu Phe Arg Asn Lys Ser Ile Ser Asn Val Gln Tyr Asn Ile
1 5 10 15
Ile Phe Cys Asn Leu Val Gly Ile Leu Pro Thr Lys Leu Gly Gly Asn
20 25 30
Ile Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly
35 40 45
Ala Thr Ser Leu Ser Ile Trp Ala Pro Ser Ser Glu Ala Ala Ala Pro
50 55 60
Glu Lys Asn Arg Tyr Tyr Ser Ile His Leu Gln Ala Asn Lys Asn Leu
65 70 75 80
Ala Trp Asp Val Ser Gly Asn Ser Pro Asp Asn Asn Arg Ala Ile Leu
85 90 95
Leu Trp Asp Gly Ala Ser Gly Asp Asn Glu Gln Phe Val Phe Phe Pro
100 105 110
Leu Asp Gly Gln Asn Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Thr
115 120 125
Val Thr Phe Gly Gly Ser Thr Pro Ala Ile Tyr Gly Asn Asn Asp Gly
130 135 140
Leu Pro Asn Ser Glu Ala Phe Gln Gln Arg Ser Trp Thr Gly Ser Pro
145 150 155 160
Thr Glu Gln Trp Tyr Leu Arg Asp Gln Gly Ser Asn Asn Tyr Glu Ile
165 170 175
Val Asn Gln Gly Asn Gln Lys Val Ala Ser Tyr Ser Trp Ala Gly Ser
180 185 190
Leu Ala Gln His Val Glu Tyr Val Asp Leu Asp Glu Ser Lys Pro Ser
195 200 205
Asp Ser Asn Arg Val Phe Tyr Ile Ser Ser Gly Pro Val Asn Lys Gly
210 215 220
Val Gln Pro Ala Tyr Gly Thr Ile Ser Leu Pro Thr Leu Pro Ala Thr
225 230 235 240
Gly Thr Arg Pro Glu Ala Pro Lys Tyr Thr Asp Gly Gly Ala Ile Asp
245 250 255
Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu
260 265 270
Ile Pro Cys Ile Met Val Lys Asp Ser Gln Ala Ser Asp Tyr Thr Lys
275 280 285
Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp
290 295 300
Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg Asn Tyr Thr
305 310 315 320
Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln Lys Met Thr Asp Thr
325 330 335
Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Ala
340 345 350
Ser Ala Ser Ile Lys Ser Glu Val Ser Lys Thr Ile Gln Thr Glu Ile
355 360 365
Ser Thr Thr Asn Thr Glu Ala Thr Glu Glu Thr Ile Thr Ser Thr Val
370 375 380
Thr Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr Glu Tyr Gln Leu Ala
385 390 395 400
Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ala Val Ser Asp Pro
405 410 415
Trp Val Val Lys Asn Asn Lys Ile Thr Val Ala Arg Lys Ile
420 425 430
<210> 289
<211> 397
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 289
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ser Ser Glu Ala Ala Ala Pro Glu
20 25 30
Lys Asn Arg Tyr Tyr Ser Ile His Leu Gln Ala Asn Lys Asn Leu Ala
35 40 45
Trp Asp Val Ser Gly Asn Ser Pro Asp Asn Asn Arg Ala Ile Leu Leu
50 55 60
Trp Asp Gly Ala Ser Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gln Asn Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Thr Val
85 90 95
Thr Phe Gly Gly Ser Thr Pro Ala Ile Tyr Gly Asn Asn Asp Gly Leu
100 105 110
Pro Asn Ser Glu Ala Phe Gln Gln Arg Ser Trp Thr Gly Ser Pro Thr
115 120 125
Glu Gln Trp Tyr Leu Arg Asp Gln Gly Ser Asn Asn Tyr Glu Ile Val
130 135 140
Asn Gln Gly Asn Gln Lys Val Ala Ser Tyr Ser Trp Ala Gly Ser Leu
145 150 155 160
Ala Gln His Val Glu Tyr Val Asp Leu Asp Glu Ser Lys Pro Ser Asp
165 170 175
Ser Asn Arg Val Phe Tyr Ile Ser Ser Gly Pro Val Asn Lys Gly Val
180 185 190
Gln Pro Ala Tyr Gly Thr Ile Ser Leu Pro Thr Leu Pro Ala Thr Gly
195 200 205
Thr Arg Pro Glu Ala Pro Lys Tyr Thr Asp Gly Gly Ala Ile Asp Gln
210 215 220
Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile
225 230 235 240
Pro Cys Ile Met Val Lys Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile
245 250 255
His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys
260 265 270
Thr Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg Asn Tyr Thr Phe
275 280 285
Lys Thr Gly Met Thr Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu
290 295 300
Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Ala Ser
305 310 315 320
Ala Ser Ile Lys Ser Glu Val Ser Lys Thr Ile Gln Thr Glu Ile Ser
325 330 335
Thr Thr Asn Thr Glu Ala Thr Glu Glu Thr Ile Thr Ser Thr Val Thr
340 345 350
Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr Glu Tyr Gln Leu Ala Thr
355 360 365
Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ala Val Ser Asp Pro Trp
370 375 380
Val Val Lys Asn Asn Lys Ile Thr Val Ala Arg Lys Ile
385 390 395
<210> 290
<211> 386
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 290
Met Lys Lys Ser Ser Phe Val Thr Gly Cys Ile Ile Thr Ala Leu Met
1 5 10 15
Ala Gly Gln Thr Val Ser Ser Gln Val Phe Ala Ser Lys Lys Gln Gln
20 25 30
Thr Asp Glu Gln Ile Asn Thr Pro Ile Ala Thr Ser Thr Thr Ser Ser
35 40 45
Ala Leu Gln Glu Leu Gly Thr Gln Thr Val Leu Leu Lys Thr His Ala
50 55 60
Leu Thr Leu Leu Lys Gln Pro Thr Ile Thr Ile Ala Thr Met Pro Asp
65 70 75 80
Leu Ser Val Tyr Gln Glu Lys Ala Lys Gln His Ala Gln Tyr Trp Leu
85 90 95
Asp Thr Val Gln Pro Ser Phe Ile Asp Ala Asn Gln Gln Leu Ile Asp
100 105 110
Phe Gln Gln Lys Phe Gln Asn Tyr Tyr Lys Lys Leu Ile Glu Leu Ser
115 120 125
Gln Lys Ile Asn Thr Asp Ala Thr Ala Arg Gln Glu Phe Ile Ile Gly
130 135 140
Ile Thr Lys Leu Gln Ala Ser Leu Ala Asn His Gln Asn Gln Ile Gln
145 150 155 160
Thr Met Ser Pro Asn Leu Gln Lys Phe Gln Glu Lys Phe Gly Thr Asp
165 170 175
Ala Gln Asn Phe Ile Asp Ala Ser Lys Thr Ala Gln Lys Ser Leu Ala
180 185 190
Asp Lys Asp Gly Glu Val Ala Gln Leu Thr Gln Arg Ile Glu Thr Leu
195 200 205
Asp Ser Asp Ile Thr Ala Gln Ile Gly Pro Ile Val Ser Gly Thr Ile
210 215 220
Ser Met Ile Ala Gly Ile Gly Ser Ile Thr Phe Gly Thr Ile Met Leu
225 230 235 240
Phe Thr Thr Gly Gly Thr Thr Ala Thr Phe Val Val Pro Met Leu Ala
245 250 255
Gly Ile Val Ala Gly Thr Gly Gly Leu Ile Gly Gly Ser Val Thr Val
260 265 270
Gly Phe Ala Ala Lys Lys Leu Glu Asp Lys Arg Lys Glu Leu Gln Ala
275 280 285
Val Thr Glu Lys Leu Thr Ala Ala Lys Ala Asp Ala Val Ala Leu Ser
290 295 300
Leu Leu Ala Gly Gln Leu Asn Val Phe Lys Asp Thr Val Ala Lys Gly
305 310 315 320
Lys Glu Ser Val Glu Ser Phe Asp Asp Asn Trp Asn Thr Leu Gln Gln
325 330 335
Asn Phe Ile Glu Leu Gln Asn Asn Val Lys Gln Ile Asn Pro Asp Ser
340 345 350
Ala Val Leu Gln Asn Arg Leu Ala Lys Ile Lys Lys Ser Val Asp Asp
355 360 365
Leu Ala Val Gln Ala Lys Gln Gln Glu Lys Val Ile Thr Ala Ile Ser
370 375 380
Tyr Gln
385
<210> 291
<211> 360
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 291
Met Ser Lys Lys Gln Gln Thr Asp Glu Gln Ile Asn Thr Pro Ile Ala
1 5 10 15
Thr Ser Thr Thr Ser Ser Ala Leu Gln Glu Leu Gly Thr Gln Thr Val
20 25 30
Leu Leu Lys Thr His Ala Leu Thr Leu Leu Lys Gln Pro Thr Ile Thr
35 40 45
Ile Ala Thr Met Pro Asp Leu Ser Val Tyr Gln Glu Lys Ala Lys Gln
50 55 60
His Ala Gln Tyr Trp Leu Asp Thr Val Gln Pro Ser Phe Ile Asp Ala
65 70 75 80
Asn Gln Gln Leu Ile Asp Phe Gln Gln Lys Phe Gln Asn Tyr Tyr Lys
85 90 95
Lys Leu Ile Glu Leu Ser Gln Lys Ile Asn Thr Asp Ala Thr Ala Arg
100 105 110
Gln Glu Phe Ile Ile Gly Ile Thr Lys Leu Gln Ala Ser Leu Ala Asn
115 120 125
His Gln Asn Gln Ile Gln Thr Met Ser Pro Asn Leu Gln Lys Phe Gln
130 135 140
Glu Lys Phe Gly Thr Asp Ala Gln Asn Phe Ile Asp Ala Ser Lys Thr
145 150 155 160
Ala Gln Lys Ser Leu Ala Asp Lys Asp Gly Glu Val Ala Gln Leu Thr
165 170 175
Gln Arg Ile Glu Thr Leu Asp Ser Asp Ile Thr Ala Gln Ile Gly Pro
180 185 190
Ile Val Ser Gly Thr Ile Ser Met Ile Ala Gly Ile Gly Ser Ile Thr
195 200 205
Phe Gly Thr Ile Met Leu Phe Thr Thr Gly Gly Thr Thr Ala Thr Phe
210 215 220
Val Val Pro Met Leu Ala Gly Ile Val Ala Gly Thr Gly Gly Leu Ile
225 230 235 240
Gly Gly Ser Val Thr Val Gly Phe Ala Ala Lys Lys Leu Glu Asp Lys
245 250 255
Arg Lys Glu Leu Gln Ala Val Thr Glu Lys Leu Thr Ala Ala Lys Ala
260 265 270
Asp Ala Val Ala Leu Ser Leu Leu Ala Gly Gln Leu Asn Val Phe Lys
275 280 285
Asp Thr Val Ala Lys Gly Lys Glu Ser Val Glu Ser Phe Asp Asp Asn
290 295 300
Trp Asn Thr Leu Gln Gln Asn Phe Ile Glu Leu Gln Asn Asn Val Lys
305 310 315 320
Gln Ile Asn Pro Asp Ser Ala Val Leu Gln Asn Arg Leu Ala Lys Ile
325 330 335
Lys Lys Ser Val Asp Asp Leu Ala Val Gln Ala Lys Gln Gln Glu Lys
340 345 350
Val Ile Thr Ala Ile Ser Tyr Gln
355 360
<210> 292
<211> 496
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 292
Met Lys Tyr Lys Asp Arg Ile Gln Ala Lys Arg Asn Tyr Lys Gln Thr
1 5 10 15
Ile Phe Ala Ala Ala Thr Thr Leu Thr Leu Gly Val Ser Ala Leu Ala
20 25 30
Gly Pro Ala Ser Ala Phe Ala Ala Glu Gln Lys Thr Ala Asp His Thr
35 40 45
Lys Thr Val Gln Ser Gln Lys Gln Ser Gln Ile Lys Val Met Ala Asn
50 55 60
Asp Asp Ser Tyr Ile Asp Ser Leu Glu Lys Phe Lys Lys Asn Phe Thr
65 70 75 80
Pro Thr Ser Thr Thr Gly Lys Phe Leu Asp Asp Leu Thr Lys Lys Gly
85 90 95
Leu Gly Ser Leu Thr Lys Ser Ala Asn Gly Gly Asn Ile Asn Tyr Asn
100 105 110
Asn Phe Leu Arg Asp Leu Thr Ile Thr Ser Met Asp Leu Leu Pro Tyr
115 120 125
Gly Gly Ala Leu Phe Ser Thr Phe Ile Ser Leu Met Trp Pro Met Glu
130 135 140
Gln Asn Asn Asn Ile Met Asp Gln Leu Val Glu Lys Phe Ser Lys Leu
145 150 155 160
Val Asp Glu Lys Ile Val Asp Tyr Asp Asn Gly Thr Leu Lys Gln Lys
165 170 175
Ser Ile Ala Leu Gln Asp Gln Leu Lys Gln Phe Asp Thr Leu Val Asn
180 185 190
Arg Ile Glu Asn Lys Val Thr Thr Tyr Met Ser Asp Arg Gln Ala Ala
195 200 205
Ala Thr Leu Ala Leu Ser Leu Asn Thr Ala Phe Asn Asp Leu Leu Arg
210 215 220
Glu Cys Gln Lys Asp Asp Gln Lys Val Ser Glu Leu Pro Leu Tyr Thr
225 230 235 240
Gln Phe Ala Thr Ala His Leu Val Phe Leu Lys Tyr Val Val Asp Asn
245 250 255
Gly Gly Lys Gly Thr Thr Leu Asp Phe Glu Lys Asn Ala Leu Gln Thr
260 265 270
Phe Lys Asp Ser Leu Asp Asn Gly Thr Lys Glu Tyr Ser Glu His Ile
275 280 285
Lys Asn Val Ala Tyr Glu Gly Leu Ala Lys Ile Ser Lys Lys Glu Leu
290 295 300
Gly Phe Val Ala Thr Asn Asp Asp Glu Tyr Thr Gln Ala Met Ala Glu
305 310 315 320
Gly Ala Arg Arg Thr Ile Val Ser Leu Glu Gly Arg Pro Leu Pro Pro
325 330 335
Glu Phe Lys Arg Gln Leu Asp Gln Ala Arg Ser Asp Leu Asn Ala Ile
340 345 350
Gln Asn Gly Lys Lys Tyr Ala Leu Pro Thr Asn Val Ala Glu Lys Leu
355 360 365
Asn Pro Tyr Arg Ala Ser Thr Val Asp Asn Leu Ala Phe Asn Thr Leu
370 375 380
Leu Ser Leu Leu Lys Gly Asn Lys His Thr Gly Trp Ile Gln Glu Asn
385 390 395 400
Gly Arg Tyr Phe Tyr Glu Lys Glu Gly Ile Arg Asp Thr Ser Gly Trp
405 410 415
Tyr Lys Asp Gly Asn Arg Tyr Tyr Tyr Leu Asp Pro Thr Asp Gly His
420 425 430
Met Thr Thr Gly Trp Leu Lys Asp Asp Gly Lys Trp Tyr Tyr Leu Ser
435 440 445
Pro Thr Glu Gln Asn Gly Phe Lys Gln Gly Gln Met Ile Phe Gln Pro
450 455 460
Lys Ala Glu Gly Asp Leu Ser Pro Ala Gln Ile Val Ile Asp Gly Lys
465 470 475 480
Gly Tyr Ser Phe Gly Pro Asp Gly Val Cys Leu Asn Pro Asp Gly Asn
485 490 495
<210> 293
<211> 551
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 293
Met Asn Tyr Lys Lys Arg Thr Asn Ala Lys Arg Lys Tyr Lys Gln Thr
1 5 10 15
Leu Phe Val Thr Ile Ala Thr Met Thr Leu Gly Gly Ser Ile Leu Gly
20 25 30
Asn Thr Ala Ser Ala Phe Ala Ala Glu Asn Thr Thr Glu Lys Gln Lys
35 40 45
Val Ser Ser Ser Pro Lys Asn Ser Asp Thr Val Gly Ser Asn Val Phe
50 55 60
Lys Val Ala Ala Asp Gly Ser Val Thr Leu Leu Asn Ser Thr Pro Phe
65 70 75 80
Asp Ser Pro Thr Val Lys Ala Ile Thr Leu Leu Gly Gly Asn Leu Leu
85 90 95
Asn Gln Ala Tyr Leu Asp Ala Tyr Asn Lys Asp Phe Thr Gly Thr Ala
100 105 110
Arg Thr Leu Val Leu Gly Ala Thr Ala Leu Ile Pro Tyr Val Gly Ser
115 120 125
Phe Ile Ser Pro Ile Ile Gly Leu Leu Trp Pro Ala Asn Val Gly Thr
130 135 140
Asp Met Thr Thr Gln Thr Lys Ala Leu Val Ala Leu Ile Asn Glu Gln
145 150 155 160
Ile Ala Asp Tyr Asp Ile Glu Thr Leu Glu Gln Lys Gly Lys Thr Leu
165 170 175
Lys Asp Leu Met Ser Arg Phe Asp Asp Leu Leu Asn Gly Lys Pro Leu
180 185 190
Thr Phe Gly Glu Gln Gly Thr Val Gln Glu Thr Leu Arg Asn Lys Ala
195 200 205
Ser Asn Ile Asn Asp Lys Phe Ile Glu Val Leu Asn Asp Cys Gln Lys
210 215 220
Arg Ser Phe Lys Ala Glu Glu Leu Pro Ile Tyr Thr Leu Ile Ala Thr
225 230 235 240
Ala His Leu Leu Phe Leu Asn Met Ile Glu Gln Asn Gly Gln Ser Ser
245 250 255
Arg Ile Gln Met Asp Asp Glu Thr Tyr Lys Gly Tyr Val Glu Asp Phe
260 265 270
Lys Arg Leu Pro Ile Glu Tyr Ala Asn Tyr Ile Glu Ala Thr Ser Lys
275 280 285
Leu Gly Lys Gln Lys Phe Asn Lys Lys Met Asp Asp Thr Ile Asn Ala
290 295 300
Ala Lys Lys Gln Ala Leu Val Gly Ile Ile Ser Pro Asp Glu Asn Thr
305 310 315 320
Asn Leu Glu Asn Leu Glu Lys Thr Arg Lys Gln Cys Leu Asp Glu Ala
325 330 335
Lys Leu Leu Gln Gln Met Gly Asp Ala Tyr Gly Ala Glu Lys Ala Arg
340 345 350
Gln Lys Ala Gln Lys Ala Tyr Glu Tyr Lys Lys Gln Tyr Glu Asp Leu
355 360 365
Ile Gln Lys Arg Gly Asn Tyr Ile Lys Asn Thr Ala Asp Asn Glu Ala
370 375 380
Phe Lys Leu Ala Lys Lys Ser Met Glu Glu Leu Met Ile Asp Val Arg
385 390 395 400
Ile Pro Asn Gly Lys Tyr Ile Ile Gln Ser Val Gln Asn Ser Asn Met
405 410 415
Val Val Gly Met Ser Gly Asn Asn Ile Gln Leu Glu Glu Asn Asn Gly
420 425 430
Gln Leu Asn Asn Lys Phe Phe Ile Ile Tyr Asp Gln Ala Arg Asn Ser
435 440 445
Tyr Val Ile Glu Thr Ser Val Glu Thr Gly Leu Ser Ser Tyr Met Ala
450 455 460
Ala Asn Ile Asp Tyr Ser Asn Val Asn Lys Ser Gly Val Asn Val Tyr
465 470 475 480
Ala Ile Leu Ser Thr Ser Ser His Glu His Pro Asn Leu Leu His Trp
485 490 495
Lys Leu Lys Arg Glu Arg Gly Ser Lys Phe Val Tyr Leu Glu Asn Leu
500 505 510
Glu Asn Gly Gln Val Leu Asp Val Ser Asn Ala Gly Thr Glu Lys Gly
515 520 525
Thr Asn Ile Ile Thr Trp Pro Leu Lys Gln Gln Gly Gln Asn Gln Arg
530 535 540
Phe Glu Leu Val Pro Thr Asn
545 550
<210> 294
<211> 513
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 294
Met Ala Glu Asn Thr Thr Glu Lys Gln Lys Val Ser Ser Ser Pro Lys
1 5 10 15
Asn Ser Asp Thr Val Gly Ser Asn Val Phe Lys Val Ala Ala Asp Gly
20 25 30
Ser Val Thr Leu Leu Asn Ser Thr Pro Phe Asp Ser Pro Thr Val Lys
35 40 45
Ala Ile Thr Leu Leu Gly Gly Asn Leu Leu Asn Gln Ala Tyr Leu Asp
50 55 60
Ala Tyr Asn Lys Asp Phe Thr Gly Thr Ala Arg Thr Leu Val Leu Gly
65 70 75 80
Ala Thr Ala Leu Ile Pro Tyr Val Gly Ser Phe Ile Ser Pro Ile Ile
85 90 95
Gly Leu Leu Trp Pro Ala Asn Val Gly Thr Asp Met Thr Thr Gln Thr
100 105 110
Lys Ala Leu Val Ala Leu Ile Asn Glu Gln Ile Ala Asp Tyr Asp Ile
115 120 125
Glu Thr Leu Glu Gln Lys Gly Lys Thr Leu Lys Asp Leu Met Ser Arg
130 135 140
Phe Asp Asp Leu Leu Asn Gly Lys Pro Leu Thr Phe Gly Glu Gln Gly
145 150 155 160
Thr Val Gln Glu Thr Leu Arg Asn Lys Ala Ser Asn Ile Asn Asp Lys
165 170 175
Phe Ile Glu Val Leu Asn Asp Cys Gln Lys Arg Ser Phe Lys Ala Glu
180 185 190
Glu Leu Pro Ile Tyr Thr Leu Ile Ala Thr Ala His Leu Leu Phe Leu
195 200 205
Asn Met Ile Glu Gln Asn Gly Gln Ser Ser Arg Ile Gln Met Asp Asp
210 215 220
Glu Thr Tyr Lys Gly Tyr Val Glu Asp Phe Lys Arg Leu Pro Ile Glu
225 230 235 240
Tyr Ala Asn Tyr Ile Glu Ala Thr Ser Lys Leu Gly Lys Gln Lys Phe
245 250 255
Asn Lys Lys Met Asp Asp Thr Ile Asn Ala Ala Lys Lys Gln Ala Leu
260 265 270
Val Gly Ile Ile Ser Pro Asp Glu Asn Thr Asn Leu Glu Asn Leu Glu
275 280 285
Lys Thr Arg Lys Gln Cys Leu Asp Glu Ala Lys Leu Leu Gln Gln Met
290 295 300
Gly Asp Ala Tyr Gly Ala Glu Lys Ala Arg Gln Lys Ala Gln Lys Ala
305 310 315 320
Tyr Glu Tyr Lys Lys Gln Tyr Glu Asp Leu Ile Gln Lys Arg Gly Asn
325 330 335
Tyr Ile Lys Asn Thr Ala Asp Asn Glu Ala Phe Lys Leu Ala Lys Lys
340 345 350
Ser Met Glu Glu Leu Met Ile Asp Val Arg Ile Pro Asn Gly Lys Tyr
355 360 365
Ile Ile Gln Ser Val Gln Asn Ser Asn Met Val Val Gly Met Ser Gly
370 375 380
Asn Asn Ile Gln Leu Glu Glu Asn Asn Gly Gln Leu Asn Asn Lys Phe
385 390 395 400
Phe Ile Ile Tyr Asp Gln Ala Arg Asn Ser Tyr Val Ile Glu Thr Ser
405 410 415
Val Glu Thr Gly Leu Ser Ser Tyr Met Ala Ala Asn Ile Asp Tyr Ser
420 425 430
Asn Val Asn Lys Ser Gly Val Asn Val Tyr Ala Ile Leu Ser Thr Ser
435 440 445
Ser His Glu His Pro Asn Leu Leu His Trp Lys Leu Lys Arg Glu Arg
450 455 460
Gly Ser Lys Phe Val Tyr Leu Glu Asn Leu Glu Asn Gly Gln Val Leu
465 470 475 480
Asp Val Ser Asn Ala Gly Thr Glu Lys Gly Thr Asn Ile Ile Thr Trp
485 490 495
Pro Leu Lys Gln Gln Gly Gln Asn Gln Arg Phe Glu Leu Val Pro Thr
500 505 510
Asn
<210> 295
<211> 408
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 295
Met Glu Thr Gln Gln Asp Asn Leu Asp Ile Ser Leu Ser Leu Ser Lys
1 5 10 15
Leu Gly Arg Gln Ser Gln Phe Ile Gln Leu Cys Val Asp Asp Ala Leu
20 25 30
Lys Arg Pro Asn Ile Gln Met Thr Glu Leu Pro Ala Leu Gln Thr Asp
35 40 45
Gln Ser Leu Ile Lys Gln Asp Met Gln Glu Trp Ser Ser Glu Leu Tyr
50 55 60
Pro His Leu Val Leu Ile Asn Ala Lys Ser Lys Gly Phe Thr Thr Lys
65 70 75 80
Phe Asn Ser Tyr Tyr Pro Asp Leu Lys Gln Phe Val Asp Ser Asn Glu
85 90 95
Asp Gln Gln Gly Phe Leu Asp Arg Leu Glu Ala Leu Gln Asp Val Val
100 105 110
Ser Ser Asn Gln Gly Lys Ile Gln Ser His Ile Asn Glu Leu Gln Ser
115 120 125
Phe Gln Ser Arg Leu Asp Asn Asp Met Lys Lys Leu Gly Asp His Ala
130 135 140
Lys Glu Gly Lys Asp Leu Leu Ser Gly Ser Gly Ser Gly Lys Ile Asp
145 150 155 160
Gln Tyr Asn Met Ala Leu Ser Glu Ala Arg Asn Lys Ile Gln Lys Asn
165 170 175
Leu Gln Glu Ile Ala Leu Leu Pro Gly Ala Leu Asn Ala Lys Gly Phe
180 185 190
Asp Ile Phe Lys Glu Val Tyr Asn Leu Ser Lys Glu Leu Met Thr Pro
195 200 205
Val Ala Glu Ser Ala Ile Ala Ala Val Asn Lys Gly Lys Glu Ile Glu
210 215 220
Lys Ser Ile Val Glu Ala Glu Asn Ser Ala Glu Lys Val Ala Lys Glu
225 230 235 240
Ala Asn Lys Ser Thr Ala Glu Ile Glu Thr Ala Lys Lys Glu Ala Arg
245 250 255
Glu Lys Ile Glu Lys Asp Lys Lys Asn Glu Leu Ala Ala Ala Ala Ala
260 265 270
Ala Lys Ala Gln Glu Tyr Asp Leu Leu Gln Met Ile Asp Ile Asp Lys
275 280 285
Ile Gln Lys Thr Tyr Asn Ser Phe Ala Glu Val Asn Lys Leu Ser Ala
290 295 300
Ser Gln Arg Ala Tyr Leu Glu Asp Leu Gln Lys Gln Asn Glu Thr Ile
305 310 315 320
Tyr Lys Leu Thr Thr Lys Leu Thr Ile Val Asp Ile Gln Lys Asp Leu
325 330 335
Leu Asn Leu Met Lys Asn Asp Ala Asp Thr Phe Thr Asn Gln Val Lys
340 345 350
Leu Glu Ile Ser Leu Leu Glu Asn Tyr Lys Lys Asp Trp Asp Gln Val
355 360 365
Lys Asp Cys Ile Thr Gln Leu Ser Thr Arg Ile Ser Asp Pro Lys Val
370 375 380
Gln Ser Ala Gln Leu Lys Arg Leu Lys Asp Leu Asn Ser Gln Leu Glu
385 390 395 400
Glu Gln Met Asn Gly Phe Asn Ser
405
<210> 296
<211> 952
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 296
Met Gln Met Val Thr Lys Ala Met Thr Pro Lys Leu Gly Ala Leu Pro
1 5 10 15
Asp Phe Ile Asp Asp Phe Asn Gly Ile Phe Gly Phe Met Asp Asn Ile
20 25 30
Thr Gly Val Met Ser Thr Ile Phe Gly Val Asp Thr Gly Asp Ser Ser
35 40 45
Ile Asp Asp Val Leu Asn Asn Glu Glu Leu Leu Lys Glu Met Gln Ala
50 55 60
Gln Met Gln Gln Met Gln Gln Ser Ile Thr Asp Met Ala Asn Asp Gln
65 70 75 80
Ile Ile Ser Glu Gly Ile Glu Asn Gln Ile Leu Ala Leu Thr Thr Asp
85 90 95
Leu Ser Thr Ser Ile Thr Ala Glu Phe Asn Lys Ile Glu Gly Ile Leu
100 105 110
Asn Thr Tyr Leu Pro Ala Ile Ser Asn Met Leu Gly Lys Val Tyr Glu
115 120 125
Gln Thr Ser Glu Ile Asn Glu Lys Val Asp Lys Leu Leu Ala Met Met
130 135 140
Thr Phe Ala Leu Lys Glu Leu Asp Tyr Ile Lys Asp Asn Val Val Leu
145 150 155 160
Asn Ser Ser Ile Val Glu Ile Thr Pro His Val Gln Lys Leu Val Tyr
165 170 175
Val Asn Lys Thr Phe Leu Ala Leu Thr Lys Ser Phe Leu Gln Asn Glu
180 185 190
Asp Leu Ser Ile Glu Asn Met Gln Glu Met Leu Glu Trp Ala Lys Ser
195 200 205
Ile Leu Ala Thr Glu Met Asn Ser Phe Glu Phe Ser Val Asp Thr Leu
210 215 220
His Thr Ile Ile Ile Gly Asp Asn Leu Tyr Lys Arg Ser Ala Leu Lys
225 230 235 240
Thr Phe Ser Asp Val Leu Leu Asp Asp Ala Asn Gln Tyr Gly Asp Phe
245 250 255
Gly Thr Pro Leu Ala Lys Phe Tyr Thr Phe Phe Ser Ser Leu Ala Thr
260 265 270
Leu Gln Ile Asn Ala Tyr Leu Cys Leu Thr Phe Ala Arg Lys Val Leu
275 280 285
Gly Leu Ser Glu Ile Asp Tyr Gln Val Thr Met Gln Asp Arg Ile Glu
290 295 300
Gln Gln Asn Gln Met Phe Val Asn Leu Ile Gln Asp Lys Asn Tyr Ser
305 310 315 320
Asn Val Leu Glu Ile Glu Gly Ile Tyr Pro Met Pro Gln Gln Ser Gly
325 330 335
Asp Cys Asn Ser Leu Asp Leu Gln Ala Lys Ser Gly Tyr Ala Leu Ile
340 345 350
Gly Leu Glu Phe Phe Met Asp Asn Gly Ile Tyr Lys Ala Lys Ala Tyr
355 360 365
Gln Ala Glu Leu Asp Lys Asn Phe Ser Ile His Ala Asp Thr Val Lys
370 375 380
Glu Ile Ile Ser Asp Asp Leu Ser Lys Val Phe Leu Asn Gln Thr Asn
385 390 395 400
Thr Ala Glu Asp Tyr Met Tyr Pro Leu Phe Gly Glu Leu Lys Gly Val
405 410 415
Pro Asn Thr Ile Ile Thr Arg Ile Gly Phe Gly Thr Lys Tyr Asp Glu
420 425 430
Ser Arg Lys Val Lys Ala Phe Ala Tyr Ile Asp Ala Asp Phe Ser Pro
435 440 445
Tyr Asp Pro Lys Ser Gly Thr Ile Ser Lys Glu Gly Thr Gln Thr Phe
450 455 460
Gln Ile Glu Gly Thr Glu Asn Lys Asn Trp Cys Tyr Asn Ala Trp Pro
465 470 475 480
Ile Gly Leu Ile Gly Asp Leu Tyr Met Thr Pro Leu Lys Ser Leu Ser
485 490 495
Leu Asn Val Asp Val Asp Ile His Ala Gly Asn Thr Leu Asn Met Ser
500 505 510
Gly Glu Ser Tyr Phe Ser Thr Ile Leu Ser Gly Glu Tyr Asn Ala Asn
515 520 525
Phe Ile Leu Phe Pro Tyr Thr Asn Ser Ser Asn Pro Ile Val Glu Asn
530 535 540
Leu Ile Gln Asn Gly Asp Phe Glu Asp Glu Asp Lys His Trp Glu Val
545 550 555 560
Asn Gly His Thr Ala Val Ile Ala Lys Gly Glu Gly Ile Asn Gly Ser
565 570 575
Lys Ala Met Lys Leu Glu Asn Gly Thr Ile Glu Gln Lys Leu Asn Leu
580 585 590
Lys Pro Tyr Thr Asn Tyr Glu Phe Thr Ala Tyr Val Lys Gly Lys Ser
595 600 605
Ala Arg Phe Leu Ile Arg Asp Asn Thr Leu Ala Asp Phe Ala Glu Gly
610 615 620
Tyr Leu Asp Ser Thr Ile Tyr Lys Gln Ile Lys Phe Arg Phe Lys Thr
625 630 635 640
Ala Met Asp Ala Ser Ser Phe Ile Val Ser Ala Thr Thr Pro Gln Glu
645 650 655
Ile Leu Val Asp Asn Ile Lys Val Cys Glu Val Thr Glu Thr Asp Asn
660 665 670
Leu Ile Asp Cys Gly Asp Phe Asp Phe Tyr Glu Ile Asn Glu Glu Asp
675 680 685
Leu Lys Ile Arg Leu Tyr Pro Asn His Trp Glu Arg Tyr Gly Gly Trp
690 695 700
His Ile Leu Ser Gly Lys Gly Met Phe Asp Ser Glu Ala Leu Thr Ile
705 710 715 720
Ile Asn Ser Gly Gly Ala Asn Gln Lys Val Gln Leu Lys Ala Asn Thr
725 730 735
Asn Tyr Ile Leu Thr Ala Tyr Val Lys Val Asp Asn Pro Asn Thr Thr
740 745 750
Ile Gln Ile Gly Cys Gly Ser Asn Gln Leu Thr Cys Asn Ser Thr Ser
755 760 765
Tyr Thr Pro Val Lys Leu Lys Phe Lys Thr Gly Glu Asp Pro Ala Thr
770 775 780
Thr Glu Asn Phe Ile Tyr Cys Ser Asn Pro Asn Asp Ser Gly Thr Val
785 790 795 800
Trp Ala Asp Asn Phe Leu Leu Cys Glu Val Pro Asn Leu Ile Val Asn
805 810 815
Gly Asn Phe Glu Gln Met Asp Leu Ser Ser Trp Ser Leu Ser Thr Thr
820 825 830
Asp Val Gly Lys Ile Tyr Leu Gln Ser Gly Arg Gly Met Ser Asn Ser
835 840 845
Asn Ala Ile Val Leu Ala Gly Glu Gly Gln Ile Ser Gln Lys Val Pro
850 855 860
Leu Lys Pro Tyr Thr Lys Tyr Arg Leu Thr Ala Tyr Val Arg Val Pro
865 870 875 880
Asn Gly Val Thr Ala Leu Leu Gly Tyr Gly Asn Gly Gly Asn Asp Asp
885 890 895
Tyr Arg Val His Cys Thr Ser Thr Glu Phe Lys Gln Glu Ser Leu Val
900 905 910
Phe Thr Thr Gly Ala Asn Pro Leu Gln Ser Glu Asp Val Ile Tyr Leu
915 920 925
Ser Thr Pro Asp Asp Gly Phe Lys Ile Thr Thr Gly Asp Asn Phe Glu
930 935 940
Leu Tyr Glu Leu Asp Gln Ile Glu
945 950
<210> 297
<211> 402
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 297
Met Arg Lys Gln Pro Tyr Lys Val Met Ala Leu Ser Thr Leu Met Ala
1 5 10 15
Val Phe Ala Ala Ser Asn Ile Met Pro Ala His Ser Phe Ala Ala Glu
20 25 30
Ser Thr Val Lys Pro Val Pro Ile His Ser Met Glu Lys Ala Asn Asn
35 40 45
Lys Asp Asp Gly Tyr Ser Leu Gly Pro Glu Gly Leu Lys Asp Ala Ile
50 55 60
Glu Arg Thr Gly Ser Asn Ala Leu Val Met Asp Leu Tyr Ala Leu Thr
65 70 75 80
Ile Ile Lys Gln Gly Asn Ala Asn Phe Ser Asn Val Thr Ser Val Asp
85 90 95
Lys Ser Leu Lys Glu Lys Val Ile Lys His Gln Asn Asp Ala Arg Thr
100 105 110
Asn Ala Lys Gln Trp Leu Asp Val Met Lys Pro Gln Ile Ile Ser Thr
115 120 125
Asn Gln Asp Ile Ile Asn Tyr Asp Thr Lys Phe Gln Asn Tyr Tyr Asp
130 135 140
Thr Leu Val Lys Ala Val Lys Asp Lys Asp Lys Asp Thr Leu Thr Lys
145 150 155 160
Gly Leu Thr Arg Leu Ser Asn Ser Ile Thr Gly Asn Lys Glu Lys Val
165 170 175
Asp His Leu Val Glu Asp Leu Lys Lys Phe Arg Ser Lys Met Thr Asp
180 185 190
Asp Thr Gln Asn Phe Lys Asn Asp Ala Asn Lys Ile Thr Ser Ile Leu
195 200 205
Ala Ser Gln Asp Ala Gly Ile Pro Leu Leu Gln Asn Gln Leu Ser Ala
210 215 220
Tyr Asn Glu Ala Val Ser Lys Tyr Asn Ala Ile Ile Ile Gly Ser Ser
225 230 235 240
Val Ala Val Ala Leu Gly Pro Val Ala Ile Val Gly Gly Thr Val Leu
245 250 255
Leu Ala Thr Gly Gly Gly Thr Val Leu Gly Val Gly Leu Ile Ala Ser
260 265 270
Gly Ile Gly Gly Ile Ala Gly Gly Thr Thr Gly Leu Ile Leu Ala Lys
275 280 285
Lys Glu Leu Asp Asn Ala Gln Ser Glu Ile Lys Asn Ile Thr Gly Gln
290 295 300
Ile Thr Thr Ala Gln Leu Glu Val Ala Gly Leu Thr Asn Ile Lys Ala
305 310 315 320
Gln Thr Glu Tyr Leu Thr Asn Thr Ile Asp Ile Ala Ile Thr Ala Leu
325 330 335
Gln Asn Ile Ser Asn Gln Trp His Thr Met Gly Ala Lys Tyr Gly Ala
340 345 350
Leu Leu Glu Asn Val Asn Thr Ile Ser Pro Glu Asp Leu Ala Phe Ile
355 360 365
Glu Glu Asp Leu Ser Val Ala Lys Asp Ser Trp Lys Asn Val Lys Asp
370 375 380
Tyr Ala Glu Lys Ile Tyr Ala Glu Asp Ile Lys Val Val Asp Asn Lys
385 390 395 400
Arg Ile
<210> 298
<211> 373
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 298
Met Ala Glu Ser Thr Val Lys Pro Val Pro Ile His Ser Met Glu Lys
1 5 10 15
Ala Asn Asn Lys Asp Asp Gly Tyr Ser Leu Gly Pro Glu Gly Leu Lys
20 25 30
Asp Ala Ile Glu Arg Thr Gly Ser Asn Ala Leu Val Met Asp Leu Tyr
35 40 45
Ala Leu Thr Ile Ile Lys Gln Gly Asn Ala Asn Phe Ser Asn Val Thr
50 55 60
Ser Val Asp Lys Ser Leu Lys Glu Lys Val Ile Lys His Gln Asn Asp
65 70 75 80
Ala Arg Thr Asn Ala Lys Gln Trp Leu Asp Val Met Lys Pro Gln Ile
85 90 95
Ile Ser Thr Asn Gln Asp Ile Ile Asn Tyr Asp Thr Lys Phe Gln Asn
100 105 110
Tyr Tyr Asp Thr Leu Val Lys Ala Val Lys Asp Lys Asp Lys Asp Thr
115 120 125
Leu Thr Lys Gly Leu Thr Arg Leu Ser Asn Ser Ile Thr Gly Asn Lys
130 135 140
Glu Lys Val Asp His Leu Val Glu Asp Leu Lys Lys Phe Arg Ser Lys
145 150 155 160
Met Thr Asp Asp Thr Gln Asn Phe Lys Asn Asp Ala Asn Lys Ile Thr
165 170 175
Ser Ile Leu Ala Ser Gln Asp Ala Gly Ile Pro Leu Leu Gln Asn Gln
180 185 190
Leu Ser Ala Tyr Asn Glu Ala Val Ser Lys Tyr Asn Ala Ile Ile Ile
195 200 205
Gly Ser Ser Val Ala Val Ala Leu Gly Pro Val Ala Ile Val Gly Gly
210 215 220
Thr Val Leu Leu Ala Thr Gly Gly Gly Thr Val Leu Gly Val Gly Leu
225 230 235 240
Ile Ala Ser Gly Ile Gly Gly Ile Ala Gly Gly Thr Thr Gly Leu Ile
245 250 255
Leu Ala Lys Lys Glu Leu Asp Asn Ala Gln Ser Glu Ile Lys Asn Ile
260 265 270
Thr Gly Gln Ile Thr Thr Ala Gln Leu Glu Val Ala Gly Leu Thr Asn
275 280 285
Ile Lys Ala Gln Thr Glu Tyr Leu Thr Asn Thr Ile Asp Ile Ala Ile
290 295 300
Thr Ala Leu Gln Asn Ile Ser Asn Gln Trp His Thr Met Gly Ala Lys
305 310 315 320
Tyr Gly Ala Leu Leu Glu Asn Val Asn Thr Ile Ser Pro Glu Asp Leu
325 330 335
Ala Phe Ile Glu Glu Asp Leu Ser Val Ala Lys Asp Ser Trp Lys Asn
340 345 350
Val Lys Asp Tyr Ala Glu Lys Ile Tyr Ala Glu Asp Ile Lys Val Val
355 360 365
Asp Asn Lys Arg Ile
370
<210> 299
<211> 209
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 299
Met Asn Thr Asp Thr Asn Lys Asn Ala Ile Tyr Tyr Ala Gly Asn Asp
1 5 10 15
Ile Tyr Ala Thr Cys Gly Asn Trp Ala Asn His Ile Gln His Ser Phe
20 25 30
Glu Ser Leu Ile Asp Arg Ala Ser Gly Asn His Glu Val Ala Ser Leu
35 40 45
Pro Leu Tyr Thr Lys Leu Ala Val Ala His Ile Thr Phe Leu Lys Tyr
50 55 60
Met His Glu His Ala Lys Gly Pro Lys Leu Lys Tyr Asp Glu Gln Ser
65 70 75 80
Leu Gln His Phe Phe Asp Lys Gly Asn Leu Glu Val Ile Thr Lys Lys
85 90 95
Tyr Ala Asp His Ile Lys Lys Val Tyr Asn Gln Gly Leu Gln Thr Phe
100 105 110
Tyr Asn Asn Ile Asp Lys Leu Asn Glu Leu Arg Thr Lys Lys Glu Asn
115 120 125
Leu Leu Ser Thr Ala Gly Asp Gly Ile Gly Lys Glu Asn Glu Leu Asn
130 135 140
Ile Leu Asn Ala Lys Ile Ala Lys Leu Glu Ala Lys Asn Ile Phe Lys
145 150 155 160
Asn Met Gln Asn Phe Ser Ser Ser Thr Trp Asp Asp Pro Thr Phe Gln
165 170 175
Ala Leu Ile Gln Gly Glu Trp Lys Leu Glu Asn Asn Ala Trp Thr Phe
180 185 190
Thr Asn Lys Lys Gly Glu Lys Lys Thr Gly Trp Leu Glu Leu Gly Ile
195 200 205
Gly
<210> 300
<211> 365
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 300
Met Gly Asp Lys Asp Gln Leu Leu Ser Pro Gly Glu Leu Ala Ala Val
1 5 10 15
Lys Asp Ser Pro Phe Leu Leu Ser Lys Gly Glu Trp Leu Ser Val Gln
20 25 30
Arg Tyr Val Thr Asn Thr Met Ala Leu Pro Ile Thr Glu Asp Val Met
35 40 45
Arg Thr Gln Leu Phe Met Pro Ala Asp Met Lys Phe Asp Asp Phe Thr
50 55 60
Pro Leu Leu Glu Gly Tyr Lys Thr Val Val Pro His Val Thr Lys Trp
65 70 75 80
Lys Thr Thr Thr Phe Pro Ala Thr Val Lys Leu Ala Gly Asp Ile Val
85 90 95
Leu Tyr Asn Lys Met Ala Lys Ala Tyr Tyr Asn Gly Leu Lys Glu Asn
100 105 110
Ile Asp Leu Leu Leu Lys Asn Pro Asn Asp Glu Lys Ala Arg Leu Glu
115 120 125
Leu Ser Ala Ser Ile Lys Met Leu Ser Gly Met Ala Lys Lys Tyr Ala
130 135 140
Asp Arg Ala Glu Thr Val His Gly Asp Ile Gln Ala Phe Ser Leu Gln
145 150 155 160
Thr Ala Thr Asp Ala Val Leu Ile Asp Asn Leu Asn Gly Val Tyr Lys
165 170 175
Gln Lys Phe Gly Ala Asn Ser Val Trp Gly Lys Lys Leu Val Asp Asp
180 185 190
Leu Asn Glu Leu Arg Asp Leu Ile Glu Thr Thr Ile Val Glu Tyr Glu
195 200 205
Tyr Asp Lys Ala Val Ala Cys Ala Thr Pro Ala Tyr Ala Trp Ile Val
210 215 220
Val Pro Pro Ile Gly Leu Ile Ala Ala Ile Ile Val Ala Gly Ile Tyr
225 230 235 240
Gly Asp Lys Ala Val Lys Ala Arg Asn Arg Leu Glu Glu Leu Arg Lys
245 250 255
Asp Leu Ala Thr Ala Gln Gly Asn Glu Lys Leu Ser Leu Met Leu Thr
260 265 270
Cys Ser Leu Gly Val Ala Arg Asp Ser Leu Ser Phe Ile Gln Arg Asp
275 280 285
Ile Gln Ala Ala Leu Pro Val Ile Gln Lys Ile Gln Gly Val Trp His
290 295 300
Ala Ile His Thr Gly Leu Asp Asp Leu Cys Glu Ile Ile His Glu Asn
305 310 315 320
Ile Asp Glu Ala Ile Ile Glu Ile Lys Lys Met Gly Ile Ala Ile Ala
325 330 335
Leu Glu Gln Trp Ser Ser Leu Ala Val Lys Ala Glu Ala Tyr Gln Ala
340 345 350
Asn Ala Tyr Ile Glu Gln Pro Lys Thr Thr Thr Glu Gly
355 360 365
<210> 301
<211> 363
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 301
Met Lys Ser Asn Thr Lys Leu Ala Trp Asp Val Ser Gly Asn Ser Ile
1 5 10 15
Asp Asn Asn Arg Ala Ile Leu Leu Trp Asp Thr Ala Ser Arg Gly Asp
20 25 30
Asn Glu Gln Phe Val Phe Phe Pro Leu Asp Gly Gln Asn Tyr Ala Ile
35 40 45
Val Asn Lys Asn Ser Gly Lys Pro Val Thr Phe Gly Gly Ser Thr Pro
50 55 60
Ala Leu Ile Thr Gly Gly Gly Leu Pro Asn Met Glu Ala Leu Gln Gln
65 70 75 80
Arg Ser Trp Thr Gly Ser Pro Thr Glu Gln Trp Tyr Leu Arg Asp Lys
85 90 95
Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly Asn Gln Lys Val Ala
100 105 110
Ser Phe Ala Tyr Thr Trp Ala Ser Gln His Val Asp Tyr Val Asp Leu
115 120 125
Asp Glu Ser Asn Pro Ser Asp Ser Asp Arg Val Phe Asn Ile Ala Lys
130 135 140
Asn Gly Phe Gly Gly Ile Gly Val Ser Ile Pro Gly Phe Asp Val Asp
145 150 155 160
Tyr Gly Thr Phe Ser Leu Pro Asn Leu Pro Ala Thr Gly Thr Arg Pro
165 170 175
Thr Val Pro Asp Tyr Asp Gly Val Ser Gly Pro Asn Gln Thr Leu Pro
180 185 190
Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Val Pro Cys Ile
195 200 205
Met Val Asn Asp Ser Gln Thr Ser Asp Tyr Thr Lys Ile His Asn Ser
210 215 220
Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Ala Ala Ser
225 230 235 240
Thr Val Leu Glu Pro Gly Ala Thr Gln Ser Ile Ser Phe Lys Thr Gly
245 250 255
Met Thr Ser Ser Asp Gln Gln Lys Met Thr Glu Thr Leu Ser Met Lys
260 265 270
Val Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp Lys Thr Ala Ala Leu
275 280 285
Lys Ala Glu Ile Ser Lys Thr Leu Gln Thr Glu Thr Ser Thr Thr Asn
290 295 300
Thr Glu Leu Ser Glu Asp Thr Val Thr Thr Asn Phe Lys Asp Glu Val
305 310 315 320
Gly Lys Arg Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys Tyr Thr
325 330 335
Leu Lys Arg Ala Asp Gly Ser Glu Val Ser Gly Ala Trp Thr Val Lys
340 345 350
Asn Asn Lys Ile Thr Glu Ile Arg Lys Ser Lys
355 360
<210> 302
<211> 369
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 302
Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Ala Gly Asn Gln Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Phe Leu Ala Asp Ala Pro Ser Glu Arg Phe Phe
35 40 45
Val His Pro Leu Asp Asn Asn Tyr Phe Ala Met Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Val Ala Gly Asn Gln Thr Gly Asn Asn Val Asn
65 70 75 80
Ile Gln Gln Phe Glu Trp Leu Gly Asp Ala Pro Ser Glu Tyr Trp Tyr
85 90 95
Phe His Arg Glu Ala Asp Gly His Tyr Val Ile Glu Ser Lys Leu Ser
100 105 110
Gly Lys Val Leu Asp Ile Ala Gly Asn Gln Thr Gly Asn Asn Ala Asn
115 120 125
Val Gln Gln Tyr Glu Phe Leu Pro Asp Ala Pro Ser Glu Arg Phe Ala
130 135 140
Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile Asn Thr Gln Pro
145 150 155 160
Leu Ser Ser Val Pro Glu Tyr Glu Thr Ile Asn Asp Gln Leu Pro Glu
165 170 175
Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val Pro Ser Ile Ser
180 185 190
Val Lys Asp Pro His Tyr Gly Ser Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Glu
210 215 220
Ser Tyr Val Leu Ala Pro Ser Glu Lys Tyr Asp Phe Val Thr Lys Thr
225 230 235 240
Gly Ile Lys Val Thr Asp Gln Glu Thr Ala Thr Arg Thr Val Ser Trp
245 250 255
Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly Phe Ser Leu Gly
260 265 270
Met Ser Ser Gln Tyr Ser Gln Glu Leu Gln Thr Ser Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Thr Gln Glu His His Ile Ile Asn Pro
290 295 300
Phe Ser Glu Arg Met Ala Tyr Ser Arg Tyr Ile Leu Thr Thr Glu Tyr
305 310 315 320
Tyr Val Gln Arg Lys Asn Gly Thr Ile Val Asn Ser Pro Trp Ala Met
325 330 335
Thr Asp Lys Thr Lys Ala His Ala Val Thr Phe Pro Lys Ser Thr Gly
340 345 350
Asn Val Leu Asn Gly Ser Thr Lys Gln Gln Ser Lys Ser Gly Ser Val
355 360 365
Asn
<210> 303
<211> 557
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 303
Met Glu Glu Lys Lys Thr Glu Val Gln Pro Gln Gln Ala Thr Pro Asn
1 5 10 15
Ser Asn Ser Leu Gly Ala Gly Gly Gly Tyr Glu Ser Asp Ala Leu Lys
20 25 30
Ala Leu Thr Asp Lys Ile Asn Met Asp Tyr Leu Lys Lys Ala Gly Ser
35 40 45
Val Ala Ala Asp Thr Leu Lys Ala Ala Tyr Lys Asp Ala His Thr Asn
50 55 60
Asn Gly Asn Tyr Asn Asn Thr Phe Arg Thr Leu Thr Met Thr Thr Ser
65 70 75 80
Ala Leu Ile Pro Tyr Gly Gly Val Leu Ile Ser Pro Ile Ile Gly Leu
85 90 95
Leu Trp Pro Glu Asp Leu Thr Ala Glu Asn Asn Lys Met Ala Ala Leu
100 105 110
Ile Lys Gln Leu Asp Glu Met Met Asp Thr Lys Val Thr Asp His Val
115 120 125
Val Arg Arg Leu Lys Thr Glu Val Asn Asp Val Lys Lys Gln Leu Glu
130 135 140
Leu Phe Glu Gln Asn Val Asn Thr Gly Lys His Lys Ser Gly Ser Gly
145 150 155 160
Leu Tyr Ser Leu Thr Glu Glu Asp Gly Asn Ala Ile Asp Ala Lys Asp
165 170 175
Ile His Lys Ala Phe Asn Ser Leu Leu Glu His Ser Lys Glu Glu Met
180 185 190
Gln Glu Val Ala Ala Leu Pro Ile Tyr Thr Ala Met Ala Thr Ala His
195 200 205
Leu Glu Phe Leu His Phe Leu Glu Gln Asn Gly Thr Lys Ser Ile Ile
210 215 220
Gly Phe Thr Ser Asn Ala Leu Asn Asn Ile Leu Lys Thr Arg Glu Gly
225 230 235 240
Leu Glu Asn Glu Thr Phe Gly Gln Lys Ile Lys Asp Leu Ala Asp Lys
245 250 255
Tyr Thr Lys His Val Gln Glu Thr Tyr Asn Ile Gly Arg Asp Lys Tyr
260 265 270
Met Ala Lys Met Gln Ala Ile Thr Thr His Ser Met Asn Glu Asn Asp
275 280 285
Glu Tyr Ala Asn Leu Ala Tyr Leu Lys Ser Leu Asp Asp Ala Arg Ser
290 295 300
Glu Met Gly Tyr Ser Gln Ser Lys Ile Asp Lys Ile Lys Asn Asp Ile
305 310 315 320
Lys Asn Tyr Gln Asn Leu Ile Lys Glu Lys Ala Asp Phe Arg Asp Gln
325 330 335
Thr Ala Glu Asn Ala Ala Phe Lys Leu Thr Ala Met Gly Lys Trp Asn
340 345 350
Gln Gln Gly Asp Lys Trp Tyr Tyr Ile Asn Asn Gln Gly Glu Asn Gln
355 360 365
Thr Gly Trp Leu Gln Asp Gly Ser Lys Tyr Tyr Tyr Phe Ser Pro Glu
370 375 380
Lys Thr Asp Lys Phe Glu Lys Gly Gln Met Val Thr Gly Lys Val Thr
385 390 395 400
Thr Asp Lys Gly Glu Thr Tyr Tyr Phe Asn Pro His Gly Gly Gln Met
405 410 415
Gln Thr Gly Trp Ile Gln Asp Asp Gly Lys Gln Tyr Tyr Phe Ser Pro
420 425 430
Ala Asn Asn Thr Lys Asn Tyr Asp Asn Glu Ser Phe Lys Glu Gly Gln
435 440 445
Met Met Thr Gly Trp Leu Ala His Leu Asp His Tyr Tyr Tyr Met Ser
450 455 460
Pro Gly Asp Asp Lys Thr Asn Pro Tyr Gln Lys Gly Lys Thr Phe Lys
465 470 475 480
Lys Gly Glu Met Trp Thr Gly Trp Leu Glu Asn Pro Lys Lys Ala Asn
485 490 495
Glu Trp Tyr Tyr Met Thr Pro Val Ala Arg Asp Tyr Lys Val Pro His
500 505 510
Leu Ile Gly Asn Asp Asp His Ile Ile Arg Pro Val Gly Ser Met Leu
515 520 525
His Asp Glu Tyr Leu Glu Lys Gln Asp Ser Gly Asp Gly Trp Arg Tyr
530 535 540
Tyr Phe Asn Glu Ser Gly Ile Asn Thr His Arg Tyr Asn
545 550 555
<210> 304
<211> 355
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 304
Met Asp Thr Pro Pro Asn Ser Gln His Thr Asn Ser Pro Asn Glu Phe
1 5 10 15
Asn Val Asn Arg Leu Lys Asp Ser Met Val Ala Leu Gly Ala Arg Met
20 25 30
Pro Val Ile Glu Gly Tyr Gly Leu Val Leu Leu Lys Gln Pro Asn Ile
35 40 45
Glu Ala Pro Ala Ile His Gly Ile Ala Val Asp Gln Gln Thr Ala Arg
50 55 60
Lys Tyr Val Gln Asp Trp Leu Asp Ile His Asn Pro Lys Leu Phe Ser
65 70 75 80
Val Asn Gln Asp Met Met Arg Phe Gly Lys Lys Phe Thr Thr Tyr Tyr
85 90 95
Asp Lys Leu Leu Glu Leu Ala Gly Gln Met Asn Glu Asn Pro Gln Ala
100 105 110
Lys Val Ala Phe Val Lys Ala Phe Asn Gly Ile Gln Asn Gln Met Gln
115 120 125
Ala Thr Phe Leu Ala Met Gln Asp Val Ser Ala Asp Leu Ser Asn Tyr
130 135 140
Lys Asn Glu Leu Asp Lys Asp Ser Lys Gln Phe Ser Thr Lys Val Gly
145 150 155 160
Arg Ala Ile Glu Leu Tyr Lys Gly Ser Asn Gly Asp Ile Glu Lys Leu
165 170 175
Arg Thr Gln Ile Glu Lys Tyr Asp Asp Glu Ile Gln Asn Glu Ile Thr
180 185 190
Lys Leu Leu Ser Leu Pro Ser Glu Asn Leu Lys Asp Leu Ile Asp Ile
195 200 205
Gly Glu Gln Ile Ala Gln Ile His Ile Asp Ala Leu Asn Lys Ser Ile
210 215 220
Asn Leu Ser Asp Val Gly Leu Leu Phe Thr Asn Ser Gly Lys Ala Leu
225 230 235 240
Asn Thr Asp Val Gln Glu Leu Asn Asn Lys Ile Thr Gln Lys Arg Gln
245 250 255
Gln Lys Met Glu Ser Ile Lys Gln Leu Ala Gly Ile Glu Ile Gln Ala
260 265 270
Thr Gln Met Ile Leu Val Asp Gln Lys Leu Asn Asn Phe Thr Lys Leu
275 280 285
Ile Lys Gln Gln Ser Gln Ser Phe Asp Lys Val Val Ser Ser Trp Glu
290 295 300
Arg Phe Asn Thr Thr Val Met Gln Val Gln Ala Ser Leu Asn Ser Asp
305 310 315 320
Lys Lys Ile Asp Ser Asn Ala Leu Gln Thr Gln Leu Asn Glu Leu Lys
325 330 335
Lys Phe Thr Asp Thr Leu Asn Ile Gln Thr Gln Glu Tyr Glu Gly Lys
340 345 350
Leu Gln Lys
355
<210> 305
<211> 369
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 305
Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Tyr Leu Lys Asp Ala Pro Ser Glu Arg Phe Phe
35 40 45
Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Phe Glu Trp Leu Gly Asp Ala Pro Ser Glu Tyr Trp Tyr
85 90 95
Phe His Arg Glu Ala Asp Gly Tyr Tyr Val Ile Glu Ser Lys His Ser
100 105 110
Gly Lys Val Leu Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
115 120 125
Val Gln Gln Phe Glu Phe Leu Ala Asp Ala Pro Ser Glu Arg Phe Ala
130 135 140
Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile Asn Thr Gln Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp Gln Leu Pro Glu
165 170 175
Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val Pro Ala Ile Ser
180 185 190
Val Lys Asp Pro His Tyr Gly Glu Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Glu
210 215 220
Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr Thr Ser Lys Thr
225 230 235 240
Gly Ile Lys Val Thr Asp Gln Glu Ala Ala Thr Lys Thr Val Ser Trp
245 250 255
Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly Phe Ser Val Gly
260 265 270
Met Ser Ser Gln Tyr Ser Thr Gln Leu Gln Ala Ser Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu Ile Lys Asn Pro
290 295 300
Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu Thr Thr Glu Tyr
305 310 315 320
Ser Val Gln Arg Lys Asn Gly Thr Leu Val Asn Ser Pro Trp Thr Met
325 330 335
Thr Asp Lys Thr Arg Thr His Ala Val Thr Tyr Pro Asn Ala Glu Glu
340 345 350
Lys Ala Leu Asn Glu Asn Ala Lys Gln Leu Ser Lys Val Glu Ser Val
355 360 365
Asn
<210> 306
<211> 459
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 306
Met Ala Glu Gln Lys Thr Ala Asp Asn Ile Lys Ala Val Gln Thr Gln
1 5 10 15
Ser Gln Ile Lys Ala Met Thr Asp Leu Asp Thr Asn Asn Phe Asp Lys
20 25 30
Phe Ile Asn Gly Gln His Phe Ser Lys Asp Ser Asp Val Gly Lys Phe
35 40 45
Leu Ile Lys Leu Lys Thr Ile Gly Ile Asn Ser Leu Thr Lys Ser Ala
50 55 60
Asn Asn Gly Thr Ile Asp Phe Asn Asn Phe Ala Arg Asp Leu Val Ile
65 70 75 80
Ser Gly Ala Gly Leu Ile Pro Phe Gly Gly Ala Ile Leu Ser Pro Phe
85 90 95
Ile Ala Met Leu Trp Pro Gln Ser Ser Gln Ser Gln Met Asp Gln Leu
100 105 110
Val Lys Lys Phe Ser Ala Met Met Asp Glu Lys Ile Val Asp Tyr Asp
115 120 125
Asn Gly Ala Leu Lys Gln Gln Ala Ile Ala Leu Gln Asp Arg Leu Ala
130 135 140
Ala Phe Glu Asn Leu Val Asn Arg Ile Glu Gln Lys Thr Ser Thr Phe
145 150 155 160
Ile Thr Asp Gln Asp Val Ala Ala Gln Gln Ala Leu Ala Leu Asn Asp
165 170 175
Ala Phe Asn Asp Leu Leu Arg Ser Cys Gln Lys Asp Glu Gln Lys Lys
180 185 190
Ser Glu Leu Pro Leu Tyr Thr Gln Ile Ala Thr Ala His Leu Val Phe
195 200 205
Leu Lys Tyr Val Val Glu Asn Gly Gly Lys Gly Thr Ile Leu Asn Phe
210 215 220
Gln Gln Asn Gly Leu Gln Leu Phe Lys Asp Ala Gln Glu Lys Ala Ile
225 230 235 240
Lys Glu Tyr Pro Gln Tyr Ile Ala Gln Val Ala Tyr Gln Ala Leu Ala
245 250 255
Gln Ile Ser Thr Lys Glu Leu Gly Phe Ser Ala Val Asp Asn Glu Glu
260 265 270
Tyr Thr His Gln Ile Tyr Ile Gly Ala Lys Lys Thr Tyr Asp Ser Leu
275 280 285
Gln Arg Pro Leu Leu Pro Lys Glu Lys Glu Gln Leu Glu Gln Ala Ile
290 295 300
Arg Met Met Asn Lys Ile Glu Gly Gly Tyr Lys Leu Ser Leu Pro Thr
305 310 315 320
Asn Ala Ala Asn Ala Val Tyr Ala Tyr Arg Ala Asn Thr Val Asp Asn
325 330 335
Leu Ala Phe Asn Thr Ile Leu Asn Glu Ser Thr Gly Asn Lys Lys Thr
340 345 350
Gly Trp Val Arg Glu Asn Asn Ile Tyr Phe Tyr Glu Lys Asp Gly Ile
355 360 365
Arg Gln Asn Ser Gly Trp Phe Lys Asp Gly Asp Ala Trp Tyr Tyr Leu
370 375 380
Asp His Lys Asp Gly His Met Ala Thr Gly Trp Val Lys Asp Asn Asp
385 390 395 400
Lys Trp Tyr Phe Leu Ser Ala Phe Asp Asp Pro Lys Tyr Asn Phe Lys
405 410 415
His Gly Gln Met Tyr Phe Gln Pro Lys Glu Thr Gly Gly Arg Lys Asn
420 425 430
Ala Ser Ile Leu Ile Asp Asp Gly Tyr Gly Lys His Tyr Asn Phe Asp
435 440 445
Glu Asn Gly Val Cys Leu Asn Pro Asn Gly Glu
450 455
<210> 307
<211> 344
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 307
Met Asn Glu Val Leu Arg Asn Ser Val Gly Gln His Ser His Val Ser
1 5 10 15
Lys Arg Ala Leu Arg Ala Arg Arg Ser Pro Val Val Phe Ile Asn Thr
20 25 30
Thr Gln Glu Glu Ala Glu Gln Phe Gln Glu Ile Val Ala Ile Asp Asp
35 40 45
Ile Asp Tyr Leu Thr Gln Ala Ile Ala Leu Thr Ala Leu Phe Asn Gly
50 55 60
Ala Ile Asp Asn Thr Thr Gly Arg Phe Ile Pro Gly Lys Ala Arg Glu
65 70 75 80
Leu Ile Ala Asn Tyr Asn Glu Ser Leu Asp Val Asn Ser Gln Pro Tyr
85 90 95
Lys Ile Gly Ile Phe Asn Thr His Gln Thr Thr Leu Thr Gln Gln Asn
100 105 110
Ser Ala Val Ser Ala Met Ile Gly Gln Ile Leu Glu Thr Leu Lys Arg
115 120 125
Val Met Gly Val Ala Leu Gly Ala Ser Ala Val Thr Gln Met Thr Ala
130 135 140
Ala Val Thr Asp Ala Phe Thr Asn Leu Asp Glu Gln Ser Gly Asp Ala
145 150 155 160
Trp Ile Phe Trp Glu Lys Lys Thr Ser Asn Lys Thr Thr Tyr Ser Tyr
165 170 175
Ala Ile Leu Phe Ala Phe Gln Asp Gly Ser Thr Gly Lys Leu Met Phe
180 185 190
Ala Leu Pro Met Ser Leu Glu Ile Glu Val Asp Val Ser Tyr Glu Arg
195 200 205
Val Leu Phe Ile Thr Val Glu Asp Lys Glu Thr Tyr Ser Val Lys Leu
210 215 220
Asp Thr Met Lys Val Gly Gln Leu Leu Phe Pro Lys Ser Pro Gly Ala
225 230 235 240
Asn Ala Leu Gln Ser Ala Arg Arg Leu Gly Thr Arg Ser Gly Ser Ala
245 250 255
Asp Leu Leu Ala Asp Pro Arg Ala Ile Thr Asp Val Val Val Thr Asn
260 265 270
Trp Ala Lys Thr Thr Thr Phe Ala Thr Ala Ala Thr Gly Phe Tyr Thr
275 280 285
Asn Asp His Pro Leu Ala Gln Val Met Ala Ser Glu Pro Asn Ile Val
290 295 300
Asn Pro Leu Tyr Glu Gly Asn Gln Tyr Leu Val Ser Phe Thr Leu Asp
305 310 315 320
Gly Ala Arg Gln Thr Leu Gly Phe Phe Leu Asn Cys Thr Leu Pro Asp
325 330 335
Gly Glu Leu Trp Phe Val Ser Ile
340
<210> 308
<211> 289
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 308
Met Asn Tyr Val Thr Phe Ser Thr Arg Glu Leu Gly Gly Lys Ser Ser
1 5 10 15
Pro Phe Ile Leu Ser Val Ala Glu Trp Lys Ala Phe Ser Asp Tyr Met
20 25 30
Lys Gln Cys Val Ser Leu Pro Thr Ser Val Asp Ile Leu Gln Glu Arg
35 40 45
Ile Cys Asn Ile Asn Glu Gly Gln Leu Arg Thr Ser Trp His Arg Leu
50 55 60
Leu Ala Glu Asp Ile Thr Tyr Pro Arg Met Val Thr Thr Gly Leu Glu
65 70 75 80
Ile Thr Thr Lys Val Lys Gln Ser Ser Arg Phe Trp Asp Ala Gly Gly
85 90 95
Arg Ala Gly Ile Ile Ser Leu Ala Gln Asn Ile Val Ala Tyr Ala Asp
100 105 110
Leu Ile Cys Ile Lys His His Asp Asp Leu Asn Gln Val Leu Leu Glu
115 120 125
Ala Gln Asn Gly Asn Leu Ser Pro Asn Ser Lys Ser Lys Phe Ile Asn
130 135 140
Ile Cys Lys Asp Leu Ser Glu His Ala Leu Arg Tyr His Gln Gln Ala
145 150 155 160
Gln Thr Met Met Thr Tyr Leu Ser Glu Phe Tyr Thr Glu Ile Glu Gly
165 170 175
Cys Lys Asp Ala His Gln Asn Cys Thr Asp Met Ile Ser Arg Ile Gly
180 185 190
Met Arg Asn His Leu Glu Tyr Ala Val Val His Asn Thr Val Thr Phe
195 200 205
Leu Leu Gly Ala Ile Arg Arg Ile Ile Pro Ser Glu Thr Lys Ile Leu
210 215 220
Pro Val Ile Arg Lys Ile His Leu Ile Leu Gly Ala Ile Ser Tyr Asp
225 230 235 240
Leu Lys Glu Leu Gly Asp Asp Ile Gly Gln Asp Leu Asp Asn Leu Pro
245 250 255
Ala Ile Ile Ala Ala Leu Glu Leu Glu Leu Ala Phe Glu Asn Trp Ser
260 265 270
Ala Ile Arg Gln Glu Ala Glu Asp Phe His Lys Asn Ala Glu Asn Phe
275 280 285
Ser
<210> 309
<211> 516
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 309
Met Ala Val Lys Ala Ser Asp Ile Ser Gln Gly Leu Gln Pro Leu Thr
1 5 10 15
Asn Ala Leu Lys Ile Lys Val Thr Asn Pro Glu Ala Leu Ala Ala Gly
20 25 30
Leu Ala Trp Thr Thr Gly Gln Ala Thr His Phe Leu Glu Lys Ser Glu
35 40 45
Met Gly Gly Met Ile Asn Trp Asn Asn Ala Val Lys Asp Leu Thr Ile
50 55 60
Ala Ser Ile Gly Leu Ile Pro Ile Gly Gly Ala Ile Leu Ser Pro Ile
65 70 75 80
Leu Ser Leu Leu Trp Pro Thr Glu Thr Ser Asn Asp Gln Met Gln Lys
85 90 95
Met Leu Asn Glu Val Ser Thr Met Ile Glu Glu Asn Ile Ala Asp Tyr
100 105 110
Asp Leu Asp Thr Leu Lys Pro Lys Val Asp Thr Leu Lys Asp Arg Ile
115 120 125
Ala Thr Phe Glu Lys Ala Ile Asn Lys Arg Leu Ala Gly Leu Pro Thr
130 135 140
Gly Gly Leu Leu Asn Ile Glu Gln Val Asn Ala Gln Asp Ala Arg Glu
145 150 155 160
Ile Asn Asn Lys Phe Lys Glu Val Leu Asn Asp Cys Ser Lys Val Lys
165 170 175
Ala Ser Thr Lys Glu Pro Tyr Thr Gly Glu Leu Pro Ile Tyr Thr Thr
180 185 190
Ile Ala Thr Gly His Leu Leu Phe Leu Lys Phe Ile Lys Glu Asn Thr
195 200 205
Asn Asn Pro Val Leu Gln Leu Asp Gln Lys Ile Ile Glu Arg Asp Phe
210 215 220
Gln Asp Asp Phe Asp Asn Ala Thr Glu Arg Tyr Ser Lys His Ile Glu
225 230 235 240
Asp Ile Tyr Ile Lys Gly Asp Gly Lys Ile Val Lys Lys Met Lys Glu
245 250 255
Ile Glu Asn Lys Thr Ala Glu Ala Gln Ser Pro Asp Asp Lys Tyr Ala
260 265 270
Asn Leu Gln Ala Leu Phe Ala Arg Leu Gln Thr Ala Lys Thr Leu Leu
275 280 285
Ser Asn Leu Thr Ala Pro Gly Thr Gln Pro Gly Phe Gly Gln Gln Ile
290 295 300
Arg Ile Asn Glu Leu Asn Ser Asp Ile Lys Met Leu Glu Lys Asp Ile
305 310 315 320
Pro Val Tyr Glu Lys Leu Ile Asp Lys Arg Gln Asp Tyr Lys Asp Thr
325 330 335
Thr Ile Asp Asn Glu Ala Phe Leu Leu Ala Ser Gly Lys Thr Pro Ser
340 345 350
Lys Thr Pro Ser Lys Thr Pro Ser Lys Thr Pro Ser Val Lys Ile Ala
355 360 365
Asp Gly Ser Tyr Lys Ile Thr Ser Met Gly Gly Asn Leu Asn Leu Asp
370 375 380
Tyr Ser Ser Tyr Ser Ser Ser Glu Pro Gly Val Val Leu Trp Asn Pro
385 390 395 400
Ser Glu Asp Lys Ser Gln His Trp Lys Leu Glu Tyr Asp Glu Ser Lys
405 410 415
Lys Ala Tyr Arg Ile Ile Ser Glu Arg Asp Pro Asn Lys Cys Ile Ala
420 425 430
Arg Asn Leu Trp Ser Gln Asn Leu Gly Val Ile Asp His His Leu Asp
435 440 445
Thr Val Phe Ile Thr Lys Lys Glu Asn Asn Asp Glu His Phe Trp Thr
450 455 460
Leu Glu Asp Ala Gly Asp Gly Tyr Phe Phe Ile His Ser Phe Ala Asp
465 470 475 480
Pro Gln Met Val Leu Asp Val Lys Gly Gly Ala Phe Asn Met Gly Asp
485 490 495
Thr Leu His Val Gln Pro Lys Ser Asn Tyr Pro Asn Ala Glu Lys Phe
500 505 510
Lys Phe Val Lys
515
<210> 310
<211> 353
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 310
Met Ala Gly Asn Thr Thr Asn Ala Thr Gln Ala Leu Ala Ser Lys Tyr
1 5 10 15
Ser Asn Tyr Gly Leu Gly Pro Glu Ala Leu Lys Lys Ala Ile Thr Glu
20 25 30
Thr Lys Ser Asn Met Leu Val Met Asp Leu Tyr Ala Leu Thr Leu Val
35 40 45
Lys Gln Pro Val Pro Asp Leu Asn Gly Leu Thr Ala Ile Ser Thr Thr
50 55 60
Leu Lys Thr Asn Val Lys Thr Asn Phe Glu Asn Ser Arg Lys Asp Ala
65 70 75 80
Asp Thr Trp Leu Asp Asp Leu Lys Pro Lys Ile Ile Ser Val Asn Gln
85 90 95
Ser Ile Ile Asn Tyr Asp Thr Lys Phe Gln Thr Tyr Cys Asn Thr Leu
100 105 110
Ile Arg Ala Ala Lys Glu Asn Asn Ile Pro Ile Leu Arg Ser Gly Leu
115 120 125
Thr Arg Leu Leu Gln Asp Ile Thr Lys Asn Lys Gly Asp Val Thr Gln
130 135 140
Leu Leu Glu Lys Leu Arg Asp Tyr Arg Ser Lys Leu Thr Thr Ser Thr
145 150 155 160
Gln Asn Phe Lys Asp Tyr Ser Asn Glu Val Thr Asn Ile Leu Thr Gly
165 170 175
Gln Asp Ala Gly Ile Pro Leu Met Glu Gln Gln Leu Ala Thr Asn Gln
180 185 190
Glu Leu Ile Asp Lys Asn Asn Lys Leu Leu Ala Ala Ser Ile Thr Gly
195 200 205
Thr Ala Val Gly Ser Trp Leu Thr Ala Thr Ile Val Leu Ala Pro Phe
210 215 220
Gly Ala Pro Ile Leu Gly Val Ser Ala Tyr Gly Val Asp Ser Leu Asn
225 230 235 240
Lys Gln Ile Ala Gln Ala Arg Asn Asn Ile Ile Glu Leu Thr Lys Asn
245 250 255
Ile Ser Glu Thr Arg Val Gln Ala Gly Lys Leu Gly Val Leu Lys Asn
260 265 270
Thr Thr Asp Glu Leu Thr Lys Thr Ile Asp Leu Ala Ile Asp Ala Leu
275 280 285
Gln Asn Ile Ser Asn Gln Trp Asn Thr Met Glu Ala Lys Tyr Asn Ser
290 295 300
Val Leu Gln Asn Leu Lys Thr Val Asp Pro Glu Phe Val Asp Phe Leu
305 310 315 320
Glu Leu Asp Leu Glu Val Ser Gln Thr Ser Trp Ala Asp Val Arg Lys
325 330 335
His Ala Glu Ala Leu Gln Asn Ser Glu Ile Lys Tyr Val Glu Asp Lys
340 345 350
Lys
<210> 311
<211> 414
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 311
Met Lys Arg Gln Lys Ile Lys Lys Phe Pro Leu Ser Lys Lys Val Ile
1 5 10 15
Thr Gly Leu Phe Ala Gly Ala Met Ala Leu Ser Ile Trp Ala Pro Ala
20 25 30
Ser Gln Ala Ala Thr Pro Glu Asn Asn Arg Tyr Tyr Ser Ile His Leu
35 40 45
Lys Ser Asn Pro Lys Lys Val Trp Asp Val Tyr Gln Asn Lys Thr Tyr
50 55 60
Asp Asn Ala Gly Ile Leu Leu Tyr Asp Gly Ser Asn Gly Asp Asn Glu
65 70 75 80
Gln Phe Val Phe Phe Pro Leu Asp Asp Gly Lys Tyr Ala Ile Val Asn
85 90 95
Lys Asn Ser Gly Lys Pro Val Gly Ile Gly Asp Pro Ile Ala Asn Asp
100 105 110
Asp Gly Val Arg Arg Met Thr Leu Phe Asn Asn Glu Gly Leu Arg Gln
115 120 125
Glu Ser Trp Thr Gly Ala Pro Asp Gln Gln Trp Tyr Leu Arg Asn Gln
130 135 140
Gly Tyr Asn Ser Tyr Glu Ile Val Asn Gln Gly Tyr Gly Lys Val Ala
145 150 155 160
Ser Tyr Ala Trp Gln Gly Thr Leu Ala Gln Asn Trp Glu Tyr Ala Asp
165 170 175
Leu Asp Glu Pro Asn Pro Ser Asp Ser Asp Arg Val Phe Asn Ile Ser
180 185 190
Asn Asn Pro Phe Gly Thr Glu Ile Asp Pro Phe Glu Gly Thr Pro Ile
195 200 205
Asn Met Lys Tyr Gly Thr Phe Ser Leu Pro Glu Leu Pro Ala Thr Gly
210 215 220
Thr Arg Pro Asp Val Pro Ala Tyr Asn Asp Gly Ala Gly Pro Tyr Gln
225 230 235 240
Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Val
245 250 255
Pro Cys Ile Met Val Asn Asp Ser Gln Ala Ser Asp Tyr Thr Lys Met
260 265 270
His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys
275 280 285
Ala Ala Ser Thr Val Leu Thr Pro Gly Ala Thr Gln Ser Ile Ser Phe
290 295 300
Lys Thr Gly Met Thr Ser Thr Asp Gln Gln Lys Met Thr Glu Thr Leu
305 310 315 320
Ser Met Lys Val Gly Ala Asp Leu Gly Leu Lys Phe Gly Asp Gln Thr
325 330 335
Ala Ser Leu Lys Ala Glu Ile Ser Lys Thr Leu Gln Thr Glu Ile Ser
340 345 350
Thr Ser Asn Thr Glu Val Thr Glu Asp Thr Val Thr Thr Ser Leu Thr
355 360 365
Gly Glu Ala Gly Lys Thr Thr Gly Tyr Thr Ile Tyr Gln Leu Ala Thr
370 375 380
Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ala Val Ser Gly Ser Trp
385 390 395 400
Ile Val Lys Asn Asn Lys Glu Thr Val Leu Arg Lys Thr Pro
405 410
<210> 312
<211> 378
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 312
Met Pro Glu Asn Asn Arg Tyr Tyr Ser Ile His Leu Lys Ser Asn Pro
1 5 10 15
Lys Lys Val Trp Asp Val Tyr Gln Asn Lys Thr Tyr Asp Asn Ala Gly
20 25 30
Ile Leu Leu Tyr Asp Gly Ser Asn Gly Asp Asn Glu Gln Phe Val Phe
35 40 45
Phe Pro Leu Asp Asp Gly Lys Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Pro Val Gly Ile Gly Asp Pro Ile Ala Asn Asp Asp Gly Val Arg
65 70 75 80
Arg Met Thr Leu Phe Asn Asn Glu Gly Leu Arg Gln Glu Ser Trp Thr
85 90 95
Gly Ala Pro Asp Gln Gln Trp Tyr Leu Arg Asn Gln Gly Tyr Asn Ser
100 105 110
Tyr Glu Ile Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Trp
115 120 125
Gln Gly Thr Leu Ala Gln Asn Trp Glu Tyr Ala Asp Leu Asp Glu Pro
130 135 140
Asn Pro Ser Asp Ser Asp Arg Val Phe Asn Ile Ser Asn Asn Pro Phe
145 150 155 160
Gly Thr Glu Ile Asp Pro Phe Glu Gly Thr Pro Ile Asn Met Lys Tyr
165 170 175
Gly Thr Phe Ser Leu Pro Glu Leu Pro Ala Thr Gly Thr Arg Pro Asp
180 185 190
Val Pro Ala Tyr Asn Asp Gly Ala Gly Pro Tyr Gln Gln Leu Pro Gln
195 200 205
Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Val Pro Cys Ile Met
210 215 220
Val Asn Asp Ser Gln Ala Ser Asp Tyr Thr Lys Met His Asn Ser Pro
225 230 235 240
Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Ala Ala Ser Thr
245 250 255
Val Leu Thr Pro Gly Ala Thr Gln Ser Ile Ser Phe Lys Thr Gly Met
260 265 270
Thr Ser Thr Asp Gln Gln Lys Met Thr Glu Thr Leu Ser Met Lys Val
275 280 285
Gly Ala Asp Leu Gly Leu Lys Phe Gly Asp Gln Thr Ala Ser Leu Lys
290 295 300
Ala Glu Ile Ser Lys Thr Leu Gln Thr Glu Ile Ser Thr Ser Asn Thr
305 310 315 320
Glu Val Thr Glu Asp Thr Val Thr Thr Ser Leu Thr Gly Glu Ala Gly
325 330 335
Lys Thr Thr Gly Tyr Thr Ile Tyr Gln Leu Ala Thr Lys Tyr Thr Leu
340 345 350
Lys Arg Ala Asp Gly Ser Ala Val Ser Gly Ser Trp Ile Val Lys Asn
355 360 365
Asn Lys Glu Thr Val Leu Arg Lys Thr Pro
370 375
<210> 313
<211> 396
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 313
Met Lys Lys Leu Pro Ile Val Thr Gly Cys Ile Ile Thr Ala Ile Leu
1 5 10 15
Thr Ser Gln Val Leu Pro Leu Pro Ala Ser Ala Ala Asp Lys Lys Gln
20 25 30
Val Asp Asn Gln Gly Ile Asn Thr Ser Asn Val Val Val Ser Ser Thr
35 40 45
Leu Gln Asn Leu Gly Thr Gln Ser Val Leu Leu Lys Thr Tyr Ala Leu
50 55 60
Thr Leu Leu Lys Gln Pro Thr Val Ala Ile Glu Thr Met Pro Asn Leu
65 70 75 80
Gly Val Tyr Gln Glu Lys Ala Lys Gln His Ala Arg Tyr Trp Leu Asp
85 90 95
Thr Val Gln Pro Ser Phe Ile Ala Ala Asn Gln Gln Leu Ile Ser Phe
100 105 110
Gln Gln Lys Phe Gln Asn Tyr Tyr Asn Lys Leu Ile Glu Leu Ser Ser
115 120 125
Lys Ile Glu Thr Asp Asn Gln Ala Lys Gln Glu Phe Ile Ile Gly Ile
130 135 140
Thr Lys Leu Gln Glu Cys Leu Asn Asn Asp Gln Asn Glu Ile Gln Lys
145 150 155 160
Met Ser Ser Gly Leu Gln Lys Phe Gln Gln Asn Phe Asn Val Asp Ala
165 170 175
Glu Asn Phe Ile Ser Leu Ala Ala Thr Ala Lys Thr Ser Leu Ala Asn
180 185 190
Lys Asn Gly Glu Val Glu Gln Leu Val Lys Gln Ile Glu Thr Leu Asn
195 200 205
Ser Asp Ile Thr Ser Gln Ile Gly Val Ile Val Gly Gly Thr Ile Gly
210 215 220
Met Val Ala Gly Ile Gly Ala Ile Val Phe Gly Thr Ile Val Leu Phe
225 230 235 240
Thr Ala Thr Val Ala Thr Gly Gly Val Gly Ala Ala Val Val Val Pro
245 250 255
Thr Leu Gly Ser Ile Ala Pro Ala Leu Gly Gly Ile Val Gly Gly Thr
260 265 270
Gly Gly Leu Ile Gly Gly Gly Val Thr Val Gly Phe Ala Ala Lys Lys
275 280 285
Leu Glu Glu Lys Arg Lys Glu Leu Gln Glu Thr Thr Gln Lys Leu Glu
290 295 300
Asn Ala Lys Ala Asp Ala Ala Ala Leu Thr Leu Leu Val Gly Gln Val
305 310 315 320
Asn Thr Phe Lys Asp Thr Val Thr Lys Gly Lys Glu Ser Leu Glu Gly
325 330 335
Phe Asp Asp Asn Trp Tyr Glu Leu Lys Asn Asn Phe Asn Val Leu Gln
340 345 350
Gln Asn Val Asn Lys Ile Asn Pro Asp Ser Ser Val Leu Gln Asn Asn
355 360 365
Leu Val Gln Ile Lys Lys Lys Val Asp Gly Leu Ala Leu Gln Ala Lys
370 375 380
Gln Gln Glu Lys Val Ile Thr Asp Ile Ser Tyr Lys
385 390 395
<210> 314
<211> 370
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 314
Met Ala Asp Lys Lys Gln Val Asp Asn Gln Gly Ile Asn Thr Ser Asn
1 5 10 15
Val Val Val Ser Ser Thr Leu Gln Asn Leu Gly Thr Gln Ser Val Leu
20 25 30
Leu Lys Thr Tyr Ala Leu Thr Leu Leu Lys Gln Pro Thr Val Ala Ile
35 40 45
Glu Thr Met Pro Asn Leu Gly Val Tyr Gln Glu Lys Ala Lys Gln His
50 55 60
Ala Arg Tyr Trp Leu Asp Thr Val Gln Pro Ser Phe Ile Ala Ala Asn
65 70 75 80
Gln Gln Leu Ile Ser Phe Gln Gln Lys Phe Gln Asn Tyr Tyr Asn Lys
85 90 95
Leu Ile Glu Leu Ser Ser Lys Ile Glu Thr Asp Asn Gln Ala Lys Gln
100 105 110
Glu Phe Ile Ile Gly Ile Thr Lys Leu Gln Glu Cys Leu Asn Asn Asp
115 120 125
Gln Asn Glu Ile Gln Lys Met Ser Ser Gly Leu Gln Lys Phe Gln Gln
130 135 140
Asn Phe Asn Val Asp Ala Glu Asn Phe Ile Ser Leu Ala Ala Thr Ala
145 150 155 160
Lys Thr Ser Leu Ala Asn Lys Asn Gly Glu Val Glu Gln Leu Val Lys
165 170 175
Gln Ile Glu Thr Leu Asn Ser Asp Ile Thr Ser Gln Ile Gly Val Ile
180 185 190
Val Gly Gly Thr Ile Gly Met Val Ala Gly Ile Gly Ala Ile Val Phe
195 200 205
Gly Thr Ile Val Leu Phe Thr Ala Thr Val Ala Thr Gly Gly Val Gly
210 215 220
Ala Ala Val Val Val Pro Thr Leu Gly Ser Ile Ala Pro Ala Leu Gly
225 230 235 240
Gly Ile Val Gly Gly Thr Gly Gly Leu Ile Gly Gly Gly Val Thr Val
245 250 255
Gly Phe Ala Ala Lys Lys Leu Glu Glu Lys Arg Lys Glu Leu Gln Glu
260 265 270
Thr Thr Gln Lys Leu Glu Asn Ala Lys Ala Asp Ala Ala Ala Leu Thr
275 280 285
Leu Leu Val Gly Gln Val Asn Thr Phe Lys Asp Thr Val Thr Lys Gly
290 295 300
Lys Glu Ser Leu Glu Gly Phe Asp Asp Asn Trp Tyr Glu Leu Lys Asn
305 310 315 320
Asn Phe Asn Val Leu Gln Gln Asn Val Asn Lys Ile Asn Pro Asp Ser
325 330 335
Ser Val Leu Gln Asn Asn Leu Val Gln Ile Lys Lys Lys Val Asp Gly
340 345 350
Leu Ala Leu Gln Ala Lys Gln Gln Glu Lys Val Ile Thr Asp Ile Ser
355 360 365
Tyr Lys
370
<210> 315
<211> 386
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 315
Met Lys Arg Asn Leu Ile Thr Gly Leu Leu Val Thr Ala Val Ser Thr
1 5 10 15
Ile Ser Leu Thr Ser Val Asn Val Tyr Ala Glu Glu Val Lys Phe Asn
20 25 30
Leu Lys Pro Thr Tyr Lys Gln Asn Glu Met Val Pro Asn Thr Val Ser
35 40 45
Asn Ser Ile Arg Met Leu Gly Ala Gln Ser Pro Leu Ala Gln Ala Tyr
50 55 60
Gly Leu Val Ile Leu Gln Gln Pro Asp Ile Lys Val Asn Ala Met Ser
65 70 75 80
Ser Leu Thr Asn His Gln Lys Phe Ala Lys Lys Asn Val Glu Glu Trp
85 90 95
Ile Asp Glu Tyr Asn Pro Lys Leu Ile Asn Leu Asn Gln Gln Met Met
100 105 110
Arg Tyr Ser Thr Arg Phe Asn Ser Tyr Tyr Ser Lys Leu Tyr Glu Leu
115 120 125
Ala Gly Lys Val Asn Glu Asp Ala Gln Ala Lys Glu Asp Phe Lys Asn
130 135 140
Ala Tyr Gly Lys Leu Leu Asp Gln Val Gln Thr Ile Gln Glu Asn Met
145 150 155 160
Asp Gln Thr Ser Leu Glu Leu Asn Arg Phe Lys Thr Gly Leu Asp Lys
165 170 175
Asp Ser Gln Ser Leu Ser Gln Lys Ala Asp Val Ala Ile Gln Ser Leu
180 185 190
Gln Gly Ser Asn Gly Asp Ile Thr Gln Leu Arg Ala Asp Ile Lys Arg
195 200 205
Ile His Glu Glu Ile Gln Ala Glu Leu Ser Ile Ile Leu Asn Arg Pro
210 215 220
Asn Gln Ile Ile Lys Gly Ser Ile Asn Ile Gly Lys Glu Val Phe Thr
225 230 235 240
Ile Thr Asn Gln Ala Ala Gln Thr Lys Thr Leu Asp Phe Val Ser Ile
245 250 255
Ser Ala Leu Ser Asp Glu Phe Ile Asn Ala Ser Asp Ser Gln Thr Lys
260 265 270
Gln Ala Ala Leu Arg Ile Gln Gln Lys Gln Lys Glu Leu Ile Pro Leu
275 280 285
Ile Gln Lys Leu Ser Glu Thr Glu Ile Gln Ala Thr Gly Ile Ile Phe
290 295 300
Ile Glu Asp Gln Val Asn Ser Phe Thr Glu Leu Ile Gly Arg Gln Val
305 310 315 320
Ser Thr Leu Asp Tyr Leu Leu Asn Asp Trp Lys Val Leu Asn Ser Asn
325 330 335
Met Asn Gln Ile Lys Leu Asn Leu Glu Thr Glu Lys Tyr Thr Asp Lys
340 345 350
Asn Ser Leu Gln Lys Asp Leu Thr Lys Leu Lys Asn Leu Asn Asp Glu
355 360 365
Ile Asn Lys Gln Thr Asn Gln Tyr Glu Glu Tyr Ile Thr Asn Ile Glu
370 375 380
Val His
385
<210> 316
<211> 361
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 316
Met Glu Glu Val Lys Phe Asn Leu Lys Pro Thr Tyr Lys Gln Asn Glu
1 5 10 15
Met Val Pro Asn Thr Val Ser Asn Ser Ile Arg Met Leu Gly Ala Gln
20 25 30
Ser Pro Leu Ala Gln Ala Tyr Gly Leu Val Ile Leu Gln Gln Pro Asp
35 40 45
Ile Lys Val Asn Ala Met Ser Ser Leu Thr Asn His Gln Lys Phe Ala
50 55 60
Lys Lys Asn Val Glu Glu Trp Ile Asp Glu Tyr Asn Pro Lys Leu Ile
65 70 75 80
Asn Leu Asn Gln Gln Met Met Arg Tyr Ser Thr Arg Phe Asn Ser Tyr
85 90 95
Tyr Ser Lys Leu Tyr Glu Leu Ala Gly Lys Val Asn Glu Asp Ala Gln
100 105 110
Ala Lys Glu Asp Phe Lys Asn Ala Tyr Gly Lys Leu Leu Asp Gln Val
115 120 125
Gln Thr Ile Gln Glu Asn Met Asp Gln Thr Ser Leu Glu Leu Asn Arg
130 135 140
Phe Lys Thr Gly Leu Asp Lys Asp Ser Gln Ser Leu Ser Gln Lys Ala
145 150 155 160
Asp Val Ala Ile Gln Ser Leu Gln Gly Ser Asn Gly Asp Ile Thr Gln
165 170 175
Leu Arg Ala Asp Ile Lys Arg Ile His Glu Glu Ile Gln Ala Glu Leu
180 185 190
Ser Ile Ile Leu Asn Arg Pro Asn Gln Ile Ile Lys Gly Ser Ile Asn
195 200 205
Ile Gly Lys Glu Val Phe Thr Ile Thr Asn Gln Ala Ala Gln Thr Lys
210 215 220
Thr Leu Asp Phe Val Ser Ile Ser Ala Leu Ser Asp Glu Phe Ile Asn
225 230 235 240
Ala Ser Asp Ser Gln Thr Lys Gln Ala Ala Leu Arg Ile Gln Gln Lys
245 250 255
Gln Lys Glu Leu Ile Pro Leu Ile Gln Lys Leu Ser Glu Thr Glu Ile
260 265 270
Gln Ala Thr Gly Ile Ile Phe Ile Glu Asp Gln Val Asn Ser Phe Thr
275 280 285
Glu Leu Ile Gly Arg Gln Val Ser Thr Leu Asp Tyr Leu Leu Asn Asp
290 295 300
Trp Lys Val Leu Asn Ser Asn Met Asn Gln Ile Lys Leu Asn Leu Glu
305 310 315 320
Thr Glu Lys Tyr Thr Asp Lys Asn Ser Leu Gln Lys Asp Leu Thr Lys
325 330 335
Leu Lys Asn Leu Asn Asp Glu Ile Asn Lys Gln Thr Asn Gln Tyr Glu
340 345 350
Glu Tyr Ile Thr Asn Ile Glu Val His
355 360
<210> 317
<211> 355
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 317
Met Ala Pro Ala Lys Asn Ala Tyr His Thr Ile Gln Leu Ala Ala Asn
1 5 10 15
Ala Trp Leu Lys Trp Asp Val Tyr Gly Asn Gly Thr Tyr Asp Asp Ala
20 25 30
Gly Ile Leu Leu Tyr Asn Gly Ser Phe Gly Asp Asn Glu Gln Phe Val
35 40 45
Phe Phe Pro Leu Asp Gly Gly Ser Tyr Ala Ile Val Asn Lys Asn Ser
50 55 60
Gly Lys Ala Val Thr Ile Gly Ala Gly Trp Val Gly Asp Asn Ala Phe
65 70 75 80
Ser Tyr Asn Ser Glu Gly Leu Lys Gln Ser Lys Trp Thr Gly Ala Ala
85 90 95
Ala Asn Lys Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile
100 105 110
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile
115 120 125
Thr Gly Pro Gln Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn
130 135 140
Asp Arg Leu Phe His Ile Pro Ala Ala Ala Ser Ser Thr Phe Ser Leu
145 150 155 160
Pro Thr Leu Pro Asn Val Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr
165 170 175
Ala Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val
180 185 190
Gly Ala Ser Leu Ile Pro Cys Ile Met Val Asn Asp Ser Gln Ala Ser
195 200 205
Asp Tyr Thr Lys Ile His Thr Ser Pro Tyr Tyr Thr Leu Val Lys Glu
210 215 220
Glu Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser
225 230 235 240
Glu Ser Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys
245 250 255
Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ser Asp Leu Gly Ile Lys
260 265 270
Phe Gly Asp Val Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile
275 280 285
Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg
290 295 300
Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln
305 310 315 320
Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly Ser Ile
325 330 335
Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Ile Thr Arg
340 345 350
Lys Thr Ser
355
<210> 318
<211> 487
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 318
Met Ala Val Pro Thr Ala Ala Tyr Ala Ala Thr Asp Ala Lys Lys Gly
1 5 10 15
Thr Glu Ile Leu Gln Ile Leu Ser Asp Gly Thr Val Lys Ile Val Asn
20 25 30
Asp Asn Leu Leu Lys Asn Ser Ser Ala Lys Glu Leu Thr Lys Leu Gly
35 40 45
Gly Thr Ala Phe Met Gln Leu Val Lys Gly Val Gln Asp Lys Gly Leu
50 55 60
Thr Ser Val Asn Asp Ala Ser Arg Glu Ile Val Leu Ala Gly Ile Ser
65 70 75 80
Ala Ile Pro Tyr Gly Gly Ala Leu Met Ser Ser Leu Ile Gly Leu Val
85 90 95
Trp Pro Ala Asp Gly Ala Asn Gln Met Arg Gln Leu Met Glu Glu Met
100 105 110
Ser Lys Met Met Asp Gln Lys Ile Glu Asp Phe Asp Lys Ala Gly Leu
115 120 125
Asp Arg Gln Ile Lys Asp Ala Gln Asp Ala Leu His Glu Leu Glu Met
130 135 140
Thr Leu Tyr Gly Val Gly Asn Val Gln Asp Gly Phe Phe Ser Val Gly
145 150 155 160
Asp Leu Gln Asp Thr Ala Arg Arg Lys Ala Glu Ala Ala His Gly Lys
165 170 175
Leu Thr Thr Val Ile Asn Ala Ala Gln Lys Ala Gly Tyr Asp Lys Ala
180 185 190
Glu Leu Pro Val Tyr Thr Ile Ala Val Thr Ala His Leu Met Phe Leu
195 200 205
Ser Phe Met Val Glu Asn Gly Glu Ser Pro Leu Phe Asn Ile Asp Ala
210 215 220
Asp Ser Met Asn Arg Thr Tyr Lys Val Tyr Leu Lys Asn Ala Leu Thr
225 230 235 240
Glu Tyr Glu Thr His Ile Glu Asp Thr Gly Asn Lys Gly Leu Ser Asp
245 250 255
Tyr Asn Gln Gln Ser Gln Leu Ala Lys Asn Ser Gln Asn Glu Ile Ser
260 265 270
Gln Leu Gln Ala Ala Ile Asn Arg Ala Asp Pro Ile Gly Lys Ala Gly
275 280 285
Leu Gln Val Thr Leu Asn Lys Ala Ala Thr Ile His Gln Glu Ile Val
290 295 300
Ser Lys Leu Glu Lys Leu Lys Asn Tyr Val Asp Ser Thr Val Gln Asn
305 310 315 320
Gln Ala Phe Gln Glu Ala Leu Lys Phe Ala Lys Thr Thr Ala Thr Lys
325 330 335
Asn Gly Ile Lys Ile Pro Ser Gln Ser Ala Gln Val Ala Asp Gly Ile
340 345 350
Tyr Ala Ile Ala Ser Ala Leu Asp Thr Thr Lys Leu Val Asp Asn His
355 360 365
Thr Asn Thr Lys Asn Val Ser Ile Tyr His Asn Thr Asp Ser Asn Asn
370 375 380
Gln Arg Trp Lys Phe Thr Phe Asp Ser Asn Lys Gln Ala Tyr Lys Ile
385 390 395 400
Glu Asn Leu Tyr Asp Ser Arg Val Leu Ala Thr Ser Ser Thr Ala Ile
405 410 415
Asn Phe Asn Val Phe Thr Thr Glu Phe Lys Gly Tyr Asp Asp Gln Phe
420 425 430
Trp Thr Leu Glu Pro Ala Gly Asp Arg Asn Tyr Ile Phe Lys Asn Lys
435 440 445
Gln Tyr Pro Asp Arg Val Leu Asp Ile Ser Gly Gly Thr Ala Asn Asp
450 455 460
Ser Thr Asn Ile Ile Ala Tyr Gln Ser Thr Asn Pro Gly Ala Asn Glu
465 470 475 480
Lys Phe Lys Leu Ile Lys Gln
485
<210> 319
<211> 479
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 319
Met Ala Thr Asp Ala Lys Lys Gly Thr Glu Ile Leu Gln Ile Leu Ser
1 5 10 15
Asp Gly Thr Val Lys Ile Val Asn Asp Asn Leu Leu Lys Asn Ser Ser
20 25 30
Ala Lys Glu Leu Thr Lys Leu Gly Gly Thr Ala Phe Met Gln Leu Val
35 40 45
Lys Gly Val Gln Asp Lys Gly Leu Thr Ser Val Asn Asp Ala Ser Arg
50 55 60
Glu Ile Val Leu Ala Gly Ile Ser Ala Ile Pro Tyr Gly Gly Ala Leu
65 70 75 80
Met Ser Ser Leu Ile Gly Leu Val Trp Pro Ala Asp Gly Ala Asn Gln
85 90 95
Met Arg Gln Leu Met Glu Glu Met Ser Lys Met Met Asp Gln Lys Ile
100 105 110
Glu Asp Phe Asp Lys Ala Gly Leu Asp Arg Gln Ile Lys Asp Ala Gln
115 120 125
Asp Ala Leu His Glu Leu Glu Met Thr Leu Tyr Gly Val Gly Asn Val
130 135 140
Gln Asp Gly Phe Phe Ser Val Gly Asp Leu Gln Asp Thr Ala Arg Arg
145 150 155 160
Lys Ala Glu Ala Ala His Gly Lys Leu Thr Thr Val Ile Asn Ala Ala
165 170 175
Gln Lys Ala Gly Tyr Asp Lys Ala Glu Leu Pro Val Tyr Thr Ile Ala
180 185 190
Val Thr Ala His Leu Met Phe Leu Ser Phe Met Val Glu Asn Gly Glu
195 200 205
Ser Pro Leu Phe Asn Ile Asp Ala Asp Ser Met Asn Arg Thr Tyr Lys
210 215 220
Val Tyr Leu Lys Asn Ala Leu Thr Glu Tyr Glu Thr His Ile Glu Asp
225 230 235 240
Thr Gly Asn Lys Gly Leu Ser Asp Tyr Asn Gln Gln Ser Gln Leu Ala
245 250 255
Lys Asn Ser Gln Asn Glu Ile Ser Gln Leu Gln Ala Ala Ile Asn Arg
260 265 270
Ala Asp Pro Ile Gly Lys Ala Gly Leu Gln Val Thr Leu Asn Lys Ala
275 280 285
Ala Thr Ile His Gln Glu Ile Val Ser Lys Leu Glu Lys Leu Lys Asn
290 295 300
Tyr Val Asp Ser Thr Val Gln Asn Gln Ala Phe Gln Glu Ala Leu Lys
305 310 315 320
Phe Ala Lys Thr Thr Ala Thr Lys Asn Gly Ile Lys Ile Pro Ser Gln
325 330 335
Ser Ala Gln Val Ala Asp Gly Ile Tyr Ala Ile Ala Ser Ala Leu Asp
340 345 350
Thr Thr Lys Leu Val Asp Asn His Thr Asn Thr Lys Asn Val Ser Ile
355 360 365
Tyr His Asn Thr Asp Ser Asn Asn Gln Arg Trp Lys Phe Thr Phe Asp
370 375 380
Ser Asn Lys Gln Ala Tyr Lys Ile Glu Asn Leu Tyr Asp Ser Arg Val
385 390 395 400
Leu Ala Thr Ser Ser Thr Ala Ile Asn Phe Asn Val Phe Thr Thr Glu
405 410 415
Phe Lys Gly Tyr Asp Asp Gln Phe Trp Thr Leu Glu Pro Ala Gly Asp
420 425 430
Arg Asn Tyr Ile Phe Lys Asn Lys Gln Tyr Pro Asp Arg Val Leu Asp
435 440 445
Ile Ser Gly Gly Thr Ala Asn Asp Ser Thr Asn Ile Ile Ala Tyr Gln
450 455 460
Ser Thr Asn Pro Gly Ala Asn Glu Lys Phe Lys Leu Ile Lys Gln
465 470 475
<210> 320
<211> 367
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 320
Met Ala Glu Thr Lys Glu Ala Thr Val Lys Thr Glu Lys Gln Pro Asn
1 5 10 15
Leu Pro Asp Lys Tyr Leu Gly Pro Glu Gly Leu Lys Lys Ala Leu Glu
20 25 30
Asp Thr Gly Ser His Val Ile Val Leu Asp Ala Tyr Ala Leu Thr Leu
35 40 45
Leu Lys Ser Pro Gln Ile Lys Leu Asp Lys Thr Leu Phe Lys Glu Gln
50 55 60
Lys Asp Lys Asp Leu Val Asp Ala Val Ile Asn Ile Gln Lys Val Ala
65 70 75 80
Gln Gly Asn Ala Thr Gln Trp Val Asp Val Leu Lys Pro Glu Leu Ile
85 90 95
Asn Val Asn Gln Gly Ile Val Asn Tyr Gly Ala Lys Phe Asp Asn Tyr
100 105 110
Tyr Gln Thr Ile Val Asp Ala Val Asp Ala Lys Asp Lys Glu Thr Thr
115 120 125
Lys Gln Gly Val Glu Arg Leu Tyr Asn Ser Val Gln Glu Asn Lys Arg
130 135 140
Lys Val Glu Gln Leu Val Glu Asn Leu Lys Asp Phe Arg Asn Lys Leu
145 150 155 160
Ile Thr Asp Thr Asn Glu Phe Gln Ser Lys Thr Asn Gln Ile Val Thr
165 170 175
Asn Leu Glu Gly Lys Asn Ala Ile Leu Pro Ser Leu Lys Gln Gln Ile
180 185 190
Asp Ala Tyr Asn Ser Gln Val Ser Ala Ser Ile Ala Met Ile Ala Ser
195 200 205
Gly Gly Val Leu Thr Leu Met Gly Thr Gly Phe Gly Val Leu Thr Val
210 215 220
Val Leu Ala Val Ser Gly Val Gly Leu Pro Phe Ala Ala Ile Thr Gly
225 230 235 240
Thr Ile Ala Ala Gly Ala Ile Gly Gly Gly Val Thr Leu Ile Val Thr
245 250 255
Gly Asn Asn Lys Leu Lys Glu Ala Asn Ala Gln Ile Ala Lys Leu Thr
260 265 270
Met Gln Leu Lys Asp Thr Asp Leu Gln Val Ala Ala Leu Lys Val Ala
275 280 285
Ser Gly Gln Thr Lys Thr Phe Ala Asp Thr Ile Asn Val Ala Ile Asp
290 295 300
Ser Ala Glu Ala Leu Lys Asp Gln Trp Thr Thr Met Gly Ser Lys Tyr
305 310 315 320
Lys Ser Leu Ile Asp Asn Ile Asp Gly Ile Ser Pro Ser Ser Leu Ala
325 330 335
Phe Ile Lys Glu Asp Leu Lys Thr Ala Lys Asp Ser Trp Val Asn Ile
340 345 350
Lys Thr Tyr Ala Asp Thr Leu Tyr Ser Lys Ile Thr Ile Gln Lys
355 360 365
<210> 321
<211> 385
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 321
Met Leu Met Lys Lys Ser Ser Ile Val Thr Gly Cys Val Met Thr Ala
1 5 10 15
Met Leu Ala Ser Gln Leu Ala Pro Leu Gln Thr Leu Ala Ala Glu Asn
20 25 30
Asn Gln Val Val Glu Gln Ala Ser Thr Ala Thr Val Thr Ser Pro Thr
35 40 45
Leu Gln Glu Leu Gly Thr Gln Ala Val Leu Leu Lys Thr Tyr Ala Leu
50 55 60
Thr Leu Leu Lys Gln Pro Thr Val Glu Ile Ala Ala Met Pro Asp Leu
65 70 75 80
Gly Ser Tyr Gln Glu Lys Ala Lys Gln His Ala Gln Tyr Trp Leu Asp
85 90 95
Ile Val Gln Pro Ser Phe Ile Asp Ala Asn Gln Gln Leu Ile Asp Phe
100 105 110
Gln Gln Lys Phe Gln Asn Tyr Tyr Asp Lys Leu Met Glu Leu Ala Lys
115 120 125
Lys Met Gly Thr Asp Glu Gln Ala Gln Gln Glu Phe Ile Ala Gly Ile
130 135 140
Thr Lys Leu Gln Ile Ser Leu Ser Asp His Gln Asn Glu Val Gln Thr
145 150 155 160
Met Ala Ser Asn Leu Gln Glu Phe Gln Lys Asn Phe Gly Ala Asp Ala
165 170 175
Gln Lys Phe Ile Asp Val Ala Lys Thr Ala Gln Ser Ser Leu Ala Asp
180 185 190
Lys Asn Gly Glu Val Ala Lys Leu Thr Thr Gln Leu Glu Thr Leu Asn
195 200 205
Ser Asp Ile Thr Glu Gln Ile Gly Asn Ile Val Lys Gly Thr Ile Gly
210 215 220
Leu Val Ala Gly Ile Gly Ala Ile Thr Phe Gly Thr Ile Val Leu Phe
225 230 235 240
Gly Thr Ala Gly Val Gly Val Ala Val Ile Ala Pro Ala Leu Gly Gly
245 250 255
Ile Val Ala Gly Thr Thr Gly Leu Val Thr Gly Gly Val Thr Val Gly
260 265 270
Phe Ala Ala Lys Lys Leu Glu Asp Lys Arg Lys Glu Leu Gln Asp Val
275 280 285
Val Gln Lys Leu Thr Ser Ala Lys Ala Asp Ala Ala Ala Leu Thr Leu
290 295 300
Leu Thr Gly Gln Ile Asp Thr Phe Lys Lys Thr Leu Glu Lys Gly Lys
305 310 315 320
Asp Ser Val Glu Asp Phe Gly Thr Ser Cys His Glu Leu Gln Gln Asn
325 330 335
Leu Thr Glu Leu Ser Asn Ser Val Asn Gln Ile His Pro Asp Ser Ala
340 345 350
Ile Leu Gln Asn Arg Leu Ala Gln Ile Lys Lys Ser Val Asp Gly Leu
355 360 365
Ala Thr Gln Ala Lys Gln Gln Glu Gln Thr Ile Thr Asp Ile Ser Tyr
370 375 380
Gln
385
<210> 322
<211> 357
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 322
Met Ala Glu Asn Asn Gln Val Val Glu Gln Ala Ser Thr Ala Thr Val
1 5 10 15
Thr Ser Pro Thr Leu Gln Glu Leu Gly Thr Gln Ala Val Leu Leu Lys
20 25 30
Thr Tyr Ala Leu Thr Leu Leu Lys Gln Pro Thr Val Glu Ile Ala Ala
35 40 45
Met Pro Asp Leu Gly Ser Tyr Gln Glu Lys Ala Lys Gln His Ala Gln
50 55 60
Tyr Trp Leu Asp Ile Val Gln Pro Ser Phe Ile Asp Ala Asn Gln Gln
65 70 75 80
Leu Ile Asp Phe Gln Gln Lys Phe Gln Asn Tyr Tyr Asp Lys Leu Met
85 90 95
Glu Leu Ala Lys Lys Met Gly Thr Asp Glu Gln Ala Gln Gln Glu Phe
100 105 110
Ile Ala Gly Ile Thr Lys Leu Gln Ile Ser Leu Ser Asp His Gln Asn
115 120 125
Glu Val Gln Thr Met Ala Ser Asn Leu Gln Glu Phe Gln Lys Asn Phe
130 135 140
Gly Ala Asp Ala Gln Lys Phe Ile Asp Val Ala Lys Thr Ala Gln Ser
145 150 155 160
Ser Leu Ala Asp Lys Asn Gly Glu Val Ala Lys Leu Thr Thr Gln Leu
165 170 175
Glu Thr Leu Asn Ser Asp Ile Thr Glu Gln Ile Gly Asn Ile Val Lys
180 185 190
Gly Thr Ile Gly Leu Val Ala Gly Ile Gly Ala Ile Thr Phe Gly Thr
195 200 205
Ile Val Leu Phe Gly Thr Ala Gly Val Gly Val Ala Val Ile Ala Pro
210 215 220
Ala Leu Gly Gly Ile Val Ala Gly Thr Thr Gly Leu Val Thr Gly Gly
225 230 235 240
Val Thr Val Gly Phe Ala Ala Lys Lys Leu Glu Asp Lys Arg Lys Glu
245 250 255
Leu Gln Asp Val Val Gln Lys Leu Thr Ser Ala Lys Ala Asp Ala Ala
260 265 270
Ala Leu Thr Leu Leu Thr Gly Gln Ile Asp Thr Phe Lys Lys Thr Leu
275 280 285
Glu Lys Gly Lys Asp Ser Val Glu Asp Phe Gly Thr Ser Cys His Glu
290 295 300
Leu Gln Gln Asn Leu Thr Glu Leu Ser Asn Ser Val Asn Gln Ile His
305 310 315 320
Pro Asp Ser Ala Ile Leu Gln Asn Arg Leu Ala Gln Ile Lys Lys Ser
325 330 335
Val Asp Gly Leu Ala Thr Gln Ala Lys Gln Gln Glu Gln Thr Ile Thr
340 345 350
Asp Ile Ser Tyr Gln
355
<210> 323
<211> 368
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 323
Met Pro Glu Asn Asn Arg Tyr Tyr Ser Ile His Leu Gln Ser Asn Lys
1 5 10 15
Asn Lys Val Trp Asp Val Phe Asn Asn Ala Thr Tyr Asn Asn Ala Gly
20 25 30
Ile Arg Leu Tyr Gly Gln Thr Asn Gly Asp Asn Gln Gln Phe Val Phe
35 40 45
Phe Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Pro Val Gly Ile Gly Asp Pro Ile Ile Ile Asp Asp Gly Val Arg
65 70 75 80
Arg Met Thr Ala Phe Asn Asn Glu Gly Leu Arg Gln Thr Ser Trp Thr
85 90 95
Gly Ala Pro Ala Glu Gln Trp Tyr Leu Arg Glu Gln Gly Thr Ser Asn
100 105 110
Tyr Glu Phe Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Trp
115 120 125
Met Gly Thr Leu Ala Glu Asn Trp Glu Tyr Val Asp Leu Asp Asn Ser
130 135 140
Asn Pro Thr Asp Gly Asp Arg Val Phe Tyr Val Ser Ala Gly Gln Val
145 150 155 160
Asn Lys Gly Asn Val Pro Phe His Gly Thr Phe Ser Leu Pro Gln Leu
165 170 175
Pro Ala Thr Gly Thr Arg Pro Asp Ala Pro Ser Tyr Thr Gly Gly Ile
180 185 190
Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser
195 200 205
Leu Ile Pro Cys Ile Met Val Asn Asp Ser Gln Thr Ser Asp Tyr Thr
210 215 220
Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp
225 230 235 240
Asp Lys Ala Ala Ser Thr Val Leu Thr Pro Gly Ala Thr Gln Ser Ile
245 250 255
Ser Phe Lys Thr Gly Met Thr Ser Thr Asp Gln Gln Lys Met Thr Glu
260 265 270
Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe Gly Asp
275 280 285
Gln Thr Ala Ser Leu Lys Ala Glu Ile Ser Lys Thr Leu Gln Thr Glu
290 295 300
Ile Ser Thr Ser Asn Thr Glu Val Thr Glu Asp Thr Val Thr Thr Ser
305 310 315 320
Leu Thr Gly Glu Ala Gly Lys Thr Ser Gly Tyr Thr Leu Tyr Gln Leu
325 330 335
Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ala Val Ser Gly
340 345 350
Ser Trp Thr Val Lys Asn Asn Lys Val Thr Val Leu Arg Lys Thr Pro
355 360 365
<210> 324
<211> 354
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 324
Met Pro Asn Leu Glu Lys Gly Ile Ala Tyr Asn Ile Ala Leu Gly Thr
1 5 10 15
Asp Pro Ser Leu Val Phe Asp Ile Leu Gly Asn Ser Thr Glu Phe Gly
20 25 30
Ser Ile Val Gly Leu Tyr Ala Lys Gly Gln Asn Ser Ile Asn Gln Gln
35 40 45
Phe Phe Phe Phe Glu Leu Asp Gly Gly Arg Met Ala Ile Val Pro Arg
50 55 60
Asn Ser Gly Arg Pro Leu Glu Pro Gly Phe Gly Ala Thr Pro Asp Thr
65 70 75 80
Leu Ile Val Gln Gly Glu Trp Thr Gly Leu Ser Ser Gln Gln Trp Gly
85 90 95
Leu Gln Asp Lys Thr Ala Ser Ser Val His Ile Phe Asn Gln Ser Asn
100 105 110
Gly Leu Val Val Ser Tyr Gly Arg Asn Asp Gln Asp Gln Phe Pro Glu
115 120 125
Leu Asn Thr Pro Asn Pro Ser Gly Pro Ala Pro Glu Arg Ala Trp Tyr
130 135 140
Leu Glu Pro Ala Tyr Gln Ala Ile Leu Pro Asn Phe Val Pro Thr Thr
145 150 155 160
Ser Ile Tyr Ser Pro Pro Gln Tyr Ser Ser Gly Ile Tyr Glu Val Leu
165 170 175
Pro Asp Gln Ser Glu Val Ala Thr Val Ser Asn Ala Leu Ile Pro Cys
180 185 190
Ile Leu Val Asn Asp Gly Ala Leu Ser Pro Glu Thr Lys Val Gln Gln
195 200 205
Ser Pro Tyr Tyr Leu Leu Ile Lys Gln Gln Phe Trp Gln Lys Ala Ala
210 215 220
Phe Gly Thr Val Ser Pro Asn Ser Ile Arg Glu Phe Ser Phe Lys Ala
225 230 235 240
Gly Thr Thr Phe Glu Asp Gln Lys Ser Met Ser Asp Thr Ile Asn Met
245 250 255
Thr Val Gly Gly Asp Phe Gly Leu Gln Phe Asn Glu Val Ser Thr Asn
260 265 270
Ala Lys Phe Gly Ile Ser Gln Gln Ile Gln Thr Ala Val Ser Thr Thr
275 280 285
Asn Glu Glu Leu Ser Glu Leu Thr Glu Thr Leu Ser Tyr Asn Asn Ile
290 295 300
Gln Lys Asp Thr Leu Val Gly Val Ser Ile Tyr Gln Leu Val Thr Arg
305 310 315 320
Tyr Val Leu Tyr Arg Ala Asn Gly Ser Leu Val Ala Gln Pro Trp Lys
325 330 335
Phe Ile Asp Asn Ala Ser Ala Val Thr Val Ile Thr Glu Gln Pro Ile
340 345 350
Gly Val
<210> 325
<211> 406
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 325
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Gly Val Gly Ala
1 5 10 15
Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Glu Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Ser Ile His Leu Gln Ala Asn Pro Asn Ile Ala
35 40 45
Trp Asp Val Tyr Lys Gln Trp Glu Ser Asp Asn Ala Gly Met Arg Leu
50 55 60
Phe Asn Asn Gly Phe Gln Asp Asn Glu Glu Phe Val Phe Phe Gln Leu
65 70 75 80
Asp Gly Gly Ser Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Gly Asp Ala Phe Ile Trp Thr Asp Gly Val Arg Asp Phe Pro
100 105 110
Ala Leu Asn Asn Gln Thr Leu Lys Gln Thr Asn Trp Thr Gly Ser Pro
115 120 125
Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile
130 135 140
Val His Gln Gly Thr Gly Lys Val Ala Ser His Ala Trp Asn Gln Ile
145 150 155 160
His Gly Pro His Ser Ala Glu Tyr Val Asp Leu Asp Asp Ser Asn Pro
165 170 175
Ser Asp Pro His Arg Val Phe Asn Ile Ala Lys Asn Pro Leu Asn Ala
180 185 190
Leu Gly Pro Glu Phe Pro Ile Pro Gly Phe Asp His Gly Thr Phe Ser
195 200 205
Leu Pro Thr Leu Pro Ala Val Gly Thr Arg Pro Lys Ala Pro Asp Tyr
210 215 220
Asp Lys Thr Gly Gly Ile Asp Gln Gln Leu Ser Glu Thr Ser Glu Ser
225 230 235 240
Ala Val Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Lys Asp Asn
245 250 255
Ser Ala Ser Asp Lys Thr Lys Ile His Asn Ser Pro Tyr Tyr Val Leu
260 265 270
Glu Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu Ile Val Ser Ala
275 280 285
Gly Gly Thr Ser Lys Tyr Thr Val Lys Arg Gly Ile Ser Thr Thr Asp
290 295 300
Gln Gln Lys Met Thr Glu Thr Leu Gly Met Ser Phe Gly Phe Asp Leu
305 310 315 320
Gly Leu Lys Phe Gly Asp Ser Ser Leu Ala Ile Lys Ser Ser Val Ser
325 330 335
Lys Thr Leu Gln Thr Glu Val Ser Lys Ser Ser Thr Glu Thr Lys Glu
340 345 350
Glu Thr Lys Asp Lys Thr Phe Val Ser Gln Ser Asn Lys Asn Thr Gly
355 360 365
Leu Thr Val Tyr Gln Leu Val Thr Arg Tyr Thr Leu Lys Arg Thr Asp
370 375 380
Gly Ser Thr Val Ser Pro Ser Trp Ser Ile Arg Asn Asp Glu Gln Thr
385 390 395 400
Leu Asp Arg Thr Ile Asn
405
<210> 326
<211> 935
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 326
Met Ala Phe Asp Ile Gly Ser Phe Tyr Arg Ile Asn Ser Lys Leu Ser
1 5 10 15
Gly Lys Val Phe Asp Ile Gln Ala Gln Ser Thr Ala Ser Asn Ala Val
20 25 30
Val Val Gln Asn Asp Trp His Asp Arg Asp Ser Gln Lys Phe Ile Phe
35 40 45
Tyr Pro Leu Asn Asn Gly Tyr Tyr Thr Val Ile Asn Lys Asn Ser Gly
50 55 60
Lys Val Phe Asp Ile Arg Ala Gln Ser Thr Ala Ser Asn Ala Glu Val
65 70 75 80
Val Gln Asn Asp Trp His Asp Arg Asp Ser Gln Tyr Phe Phe Thr His
85 90 95
Gln Gln Glu Ser Gly Phe Phe Ser Val Gln Ser Arg Leu Ser Gly Lys
100 105 110
Val Phe Asp Ile Arg Ala Gln Ser Thr Ala Ser Asn Ala Val Val Val
115 120 125
Gln Asn Asp Trp His Asp Arg Asp Ser Gln Lys Phe Ser Phe Ser Pro
130 135 140
Val Glu Ser Ile Ser Val Pro Ser Ile Gln Thr Gln Pro Leu Pro Pro
145 150 155 160
Pro Pro Phe Tyr Asn Gly Ile Asn Asp Ile Leu Pro Asp Arg Thr Glu
165 170 175
Pro Val Val Thr Asn Tyr Thr Leu Ala Pro Cys Ile Ala Val Asp Asp
180 185 190
Pro Tyr Tyr Asn Asp Gln Gln Lys Ile Gln Thr Gly Asn Glu Tyr Tyr
195 200 205
Leu Tyr Val Arg Glu Gln Tyr Trp Gln Arg Val Phe Ser His Val Leu
210 215 220
Val Pro Gly Glu Ser Tyr Thr Tyr Ser Gln Thr Ser Gly Met Thr Thr
225 230 235 240
Thr Asp Gln Asn Thr Val Thr Asn Thr Val Ser His Ser Ile Gly Ala
245 250 255
Asp Val Gly Phe Ser Phe Lys Gln Gly Ser Leu Ser Ala Gly Leu Ser
260 265 270
Tyr Gln Tyr Thr Gln Glu Leu Ser Val Thr Glu Ser His Thr Glu Glu
275 280 285
Leu Leu Thr Ser Thr Thr Lys Thr Phe Glu Ile Asn Asn Asn Ser Arg
290 295 300
Glu Pro Val Ala Tyr Ser Gln Tyr Leu Leu Val Ser Glu Tyr Tyr Val
305 310 315 320
Arg Arg Ala Asp Gly Thr Ile Val Asn Asp Arg Trp Ala Val Ser Asp
325 330 335
Ala Asp Ser Thr Val Thr Val Ser Tyr Pro Ser Asn Val Glu Leu Pro
340 345 350
Ile Leu Glu Leu Asp Ser Gly Phe Val Asp Ala Gln Gln Ala Val Arg
355 360 365
Asn Leu Phe Ala Asp Ala Glu His Ser Arg Leu Lys Ile Asp Thr Thr
370 375 380
Asp Tyr Met Ile Asp Gln Ala Ala Leu Gln Val Glu Gln Leu Ser Asp
385 390 395 400
Glu Ser Asp Glu Leu Glu Lys Met Met Leu Phe Asn Val Val Arg Cys
405 410 415
Ala Lys Gln Val Ser Lys Lys Arg Asn Leu Leu Arg Asp Gly Asp Phe
420 425 430
Glu Ser Pro Asn Trp Gln Asn Pro Glu Gly Gly Trp Gln Ala Ser Tyr
435 440 445
Asn Val Thr Val Leu Ser Asp Asn Pro Ile Phe Lys Gly Asn Tyr Val
450 455 460
Asp Met Pro Gly Ala Asn Ala Arg Asn Gly Ser Ile Thr Pro Thr Tyr
465 470 475 480
Leu Tyr Gln Lys Val Asp Glu Ser Lys Leu Lys Pro Tyr Thr Arg Tyr
485 490 495
Leu Val Arg Gly Phe Val Gly Ser Ser Lys Glu Leu Glu Leu Phe Val
500 505 510
Thr Arg Tyr Gly Lys Glu Val His Asp Lys Met Asn Ile Pro Thr Ser
515 520 525
Pro Met Asn Thr Cys Asn Gln Thr Asp Asn Tyr Ser Leu Gly Tyr Gly
530 535 540
Ser Gly Tyr Thr Met Ser Asn Asp Pro Cys Gln Leu Tyr Pro Thr Asn
545 550 555 560
Pro Met Pro Ser Ser Asn Gly Leu Cys Glu Glu Lys Gln Asn Phe Val
565 570 575
Phe His Ile Asp Val Gly Glu Ile Asn Pro Arg Ala Asp Leu Gly Ile
580 585 590
Gly Val Gly Phe Asn Ile Ser Ser Pro Asp Gly Met Ala Gln Leu Ser
595 600 605
Asn Val Glu Val Ile Glu Ala Asn Pro Leu Thr Gly Glu Ala Leu Ala
610 615 620
Arg Val Lys Lys Arg Glu Gln Lys Trp Arg Arg Glu Met Glu Lys Lys
625 630 635 640
Cys Ala Leu Thr Glu Lys Thr Val Ser Val Ala Thr Gln Ala Val His
645 650 655
Arg Leu Phe Thr Asp Ala Gln Lys Asn Arg Leu Lys Ala Thr Thr Thr
660 665 670
Met Gln Asp Ile Arg Asn Ala Glu Ala Lys Val Lys Ala Ile Pro Phe
675 680 685
Val Tyr Asn Thr Tyr Phe Glu Glu Val Pro Gly Met Asn Asp Ala Ile
690 695 700
Phe Gln Ala Leu Gln Ser Asp Val Tyr Thr Ala Ser Gly Leu Tyr Ile
705 710 715 720
Asp Arg Asn Ile Ile Arg Asn Gly Asp Phe Gly Ser Arg Leu Ser Asn
725 730 735
Trp His Ala Thr Ala Gly Ala Asp Val Gln Asp Arg Asp Gly Arg Ser
740 745 750
His Val Leu Val Ile Ser Asn Trp Ser Ala Asn Val Ser Gln Glu Val
755 760 765
Cys Val Gln Pro Glu Arg Gly Tyr Val Leu Arg Val Thr Ala Arg Lys
770 775 780
Glu Gly Ser Gly Lys Gly Tyr Val Thr Leu Thr Asp Cys Thr Ala Glu
785 790 795 800
Asn Thr Glu Thr Val Thr Phe Thr Ala Asn Glu Asp Val Thr Ile Pro
805 810 815
Arg Pro Pro Val Lys Pro Arg Gln Ser Val Thr Ser Pro Val Cys Asp
820 825 830
Met Ser Arg Tyr Ala Glu Ser Phe Gly Ile Val Pro Asp Asp Thr Asn
835 840 845
Met Met Asn Arg Ser Gln Ala Ser Tyr Gly Thr Ala Ser Cys Ser Cys
850 855 860
Gly Cys Gly Lys Thr Ile Ser Asn Gly Glu Gly Ser Cys Gln Thr Asn
865 870 875 880
Pro Cys Pro Ser Thr Pro Ser Met Pro Ile Gly Asn Gly Pro Ala Ser
885 890 895
Asn Tyr Ile Thr Lys Thr Ile Glu Ile Phe Pro Glu Thr Asn Arg Val
900 905 910
Arg Ile Glu Ile Gly Glu Thr Glu Gly Ser Phe Leu Val Glu Ser Val
915 920 925
Glu Leu Ile Cys Met Glu Glu
930 935
<210> 327
<211> 355
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 327
Met Ala Pro Ala Lys Asn Ala Tyr His Ser Ile His Leu Ala Ala Asn
1 5 10 15
Ala Asn Leu Lys Trp Asp Val Tyr Gly Ser Ser Pro Asn Asp Glu Ala
20 25 30
Gly Ile Ala Leu Tyr Asn Gly Ser Leu Arg Asp Asn Glu Gln Phe Val
35 40 45
Phe Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser
50 55 60
Gly Lys Ala Val Thr Ile Gly Ala Gly Trp Val Gly Asp Asn Ala Phe
65 70 75 80
Ser Tyr Asn Ser Glu Gly Leu Lys Gln Ser Lys Trp Thr Gly Ala Ala
85 90 95
Ala Asn Lys Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile
100 105 110
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile
115 120 125
Thr Gly Pro Gln Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn
130 135 140
Asp Arg Leu Phe His Ile Pro Ala Ala Ala Arg Ser Thr Phe Ser Leu
145 150 155 160
Pro Thr Leu Pro Thr Ile Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr
165 170 175
Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val
180 185 190
Gly Ala Ser Leu Leu Pro Cys Ile Met Val Asn Asp Ser Gln Val Asp
195 200 205
Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu
210 215 220
Glu Tyr Trp Asp Lys Thr Phe Ser Lys Val Val Gln Pro Gly Leu Ser
225 230 235 240
Glu Ser Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys
245 250 255
Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ala Asp Leu Gly Ile Lys
260 265 270
Phe Gly Asp Val Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile
275 280 285
Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg
290 295 300
Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln
305 310 315 320
Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Thr
325 330 335
Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg
340 345 350
Lys Thr Ser
355
<210> 328
<211> 369
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 328
Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Tyr Leu Lys Asp Ala Pro Ser Glu Arg Phe Phe
35 40 45
Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Phe Glu Trp Leu Gly Asp Ala Pro Ser Glu Tyr Trp Tyr
85 90 95
Phe His Arg Glu Ala Asp Gly Tyr Tyr Val Ile Glu Ser Lys His Ser
100 105 110
Gly Lys Val Leu Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
115 120 125
Val Gln Gln Phe Glu Phe Leu Ala Asp Ala Pro Ser Glu Arg Phe Ala
130 135 140
Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile Asn Thr Gln Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp Gln Leu Pro Glu
165 170 175
Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val Pro Ala Ile Ser
180 185 190
Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Glu
210 215 220
Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr Thr Ser Lys Thr
225 230 235 240
Gly Ile Lys Val Thr Asp Gln Glu Thr Ala Thr Lys Thr Val Ser Trp
245 250 255
Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly Phe Ser Val Gly
260 265 270
Met Ser Ser Gln Tyr Ser Thr Gln Leu Gln Thr Ser Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu Ile Lys Asn Pro
290 295 300
Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu Thr Thr Glu Tyr
305 310 315 320
Ser Val Gln Arg Lys Asn Gly Thr Ile Val Asn Ser Pro Trp Thr Met
325 330 335
Thr Asp Lys Thr Arg Thr His Ala Val Thr Tyr Pro Asn Ala Glu Glu
340 345 350
Lys Ala Leu Asn Glu Asn Lys Lys Gln Leu Ser Lys Ala Glu Ser Val
355 360 365
Asn
<210> 329
<211> 370
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 329
Met Thr Phe Lys Val Gly Thr Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Ala Gly Asn Arg Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Tyr Leu Glu Lys Ala Asp Ser Glu Arg Phe Phe
35 40 45
Leu His Lys Leu Asp Asp Asp Tyr Tyr Ala Met Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ala Ser Ser Gln Tyr Trp Lys
85 90 95
Phe Ile Pro Glu Thr Glu Gly Tyr Tyr Val Ile Lys Ser Lys Leu Ser
100 105 110
Gly Lys Val Leu Asp Ile Ser Gly Asn Arg Thr Asp Asn Asn Ala Asn
115 120 125
Val Gln Gln Tyr Glu Tyr Leu Glu Lys Ala Asp Ser Glu Arg Phe Ala
130 135 140
Val Glu Glu Ala Gly Ser Val Pro Leu Pro Ser Ile Asp Lys Lys Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Lys Thr Ile Asn Asp Gln Leu Pro Glu
165 170 175
Glu Thr Glu His Val Val Arg Ala Phe Ala Arg Val Pro Ala Ile Ser
180 185 190
Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Gln
210 215 220
Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys His Thr Val Arg Thr
225 230 235 240
Gly Ile Lys Val Ile Asp Gln Glu Thr Ala Thr Lys Thr Val Ser Trp
245 250 255
Ser Ile Gly Ala Asp Met Gly Leu Asp Phe Lys Gly Phe Ser Ala Gly
260 265 270
Met Ser Ser Gln Tyr Ser Glu Gln Leu Glu Thr Thr Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Ile Met Asp His Glu Ile Arg Asn Thr
290 295 300
Thr Thr Asp Asn Met Ala Tyr Ser Arg Tyr Ile Leu Val Thr Glu Tyr
305 310 315 320
Ser Val Ile Arg Pro Lys Asp Gly Ser Thr Val Asn Ser Ser Trp Thr
325 330 335
Met Thr Asp Lys Asn Asn Ala His Ala Val Tyr Phe Pro Lys Ser Ile
340 345 350
Gly Asn Leu Leu Asn Glu Ser Thr Lys Gln Leu Ser Lys Thr Glu Lys
355 360 365
Val Lys
370
<210> 330
<211> 377
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 330
Met Ala Ser Pro Glu Lys Asn Arg Tyr Tyr Ser Ile His Leu Lys Ala
1 5 10 15
Asn Pro Lys Leu Ala Trp Asp Val Ser Gly Asn Ser Leu Asp Asn Asp
20 25 30
Arg Ala Ile Val Leu Trp Asn Thr Ala Ser Arg Gly Asp Asn Glu Gln
35 40 45
Phe Val Phe Phe Pro Leu Asp Gly Gln Asn Tyr Ala Ile Val Asn Lys
50 55 60
Asn Ser Gly Lys Pro Val Thr Phe Gly Ala Gly Ala Leu Leu Glu Gly
65 70 75 80
His Asn Gly Ile Met Asp Gly Gln Gly Leu Lys Gln Lys Ser Trp Thr
85 90 95
Gly Ala Ala Thr Glu Gln Trp Tyr Leu Arg Asp Gln Gly Ser Asn Asn
100 105 110
Tyr Glu Ile Val Asn Gln Gly Asn Gln Lys Val Ala Ser Tyr Gly Trp
115 120 125
Gln Gly Ser Leu Ala Asp Tyr Ala Glu Tyr Val Lys Leu Asp Glu Ser
130 135 140
Lys Pro Ser Asp Ser Asn Arg Val Phe Asn Ile Ala Lys Asn Gly Phe
145 150 155 160
Gly Gly Val Gly Gly Asp Ile Gln Ile Pro Gly Phe Asn Leu Asp Tyr
165 170 175
Gly Thr Phe Ser Leu Pro Thr Leu Pro Ala Thr Gly Thr Arg Pro Ala
180 185 190
Ala Pro Asp Tyr Thr Thr Ala Asp Pro Asn Gln Gln Leu Pro Gln Ala
195 200 205
Ser Asn Ser Val Val Val Gly Ala Ser Leu Val Pro Cys Ile Met Val
210 215 220
Asn Asp Ser Gln Thr Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr
225 230 235 240
Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Lys Ala Ala Ser Thr Val
245 250 255
Leu Thr Pro Gly Ala Thr Gln Ser Val Ala Phe Lys Thr Gly Met Ser
260 265 270
Ser Ser Asp Gln Gln Lys Met Thr Glu Thr Leu Ser Met Lys Val Gly
275 280 285
Ala Asp Phe Gly Leu Lys Phe Gly Asp Lys Thr Ala Ala Leu Lys Ala
290 295 300
Glu Ile Thr Lys Thr Leu Gln Thr Glu Thr Ser Thr Thr Asn Thr Glu
305 310 315 320
Leu Ser Glu Asp Thr Val Thr His Asn Phe Lys Asp Glu Val Gly Lys
325 330 335
Asp Thr Gly Tyr Thr Leu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys
340 345 350
Arg Ala Asp Gly Ser Val Val Ser Gly Ser Trp Thr Val Lys Asn Asn
355 360 365
Lys Ile Thr Lys Val Ser Lys Ser Lys
370 375
<210> 331
<211> 463
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 331
Met Glu Glu Lys Asp Lys Asn Ala Thr Gln Gln Gln Asn Thr Val Gln
1 5 10 15
Gln Lys Asp Ala Gly Thr Tyr Tyr Glu Gly Glu Gln Lys Glu Leu Asn
20 25 30
Pro Leu Ala Gln Leu Asp Lys Trp Thr Gln Asp Leu Gly Lys Ala Ser
35 40 45
Gly Ala Gly Asp Tyr Lys Thr Thr Leu Gly Met Val Glu Lys Leu Leu
50 55 60
Pro Thr Ile Tyr Lys Asp Leu Asn Ser Gly Asn Phe Asn Asn Thr Ala
65 70 75 80
Arg Ser Phe Thr Met Leu Ser Thr Ala Leu Ile Pro Tyr Gly Gly Ala
85 90 95
Phe Ile Ser Pro Ile Val Gly Leu Ile Trp Pro Glu Asn Thr Thr Ala
100 105 110
Gln Asp Ala Lys Leu Gln Lys Met Val Glu Gly Leu Thr Ser Met Met
115 120 125
Gly Gln Lys Ile Asp Asp Tyr Asp Leu Ala Lys Leu Lys Gln Lys Thr
130 135 140
Asp Ala Leu Thr Asp Thr Leu Lys Lys Phe Gln Asn Ser Val Asn Asn
145 150 155 160
Lys Pro Gln Ser Glu Ser Tyr Tyr Asp Asn Ile Gln Thr Ala Asn Val
165 170 175
Thr Tyr Ala Asp Tyr Ile Asn Thr Lys Phe Glu Glu Ile Ile Lys Glu
180 185 190
Ser Gln Thr Pro Gly His Gln Ile Pro Glu Leu Pro Ile Phe Thr Val
195 200 205
Ile Ala Thr Ala His Leu Gln Phe Leu His Phe Ile Glu Glu Asn Ala
210 215 220
Val Lys Asn Pro Arg Ile Lys Met Asp Pro Glu His Leu Asn Arg Tyr
225 230 235 240
Phe Pro Lys Phe Lys Asp Thr Ala Glu Lys Tyr Arg Lys Tyr Ile Asn
245 250 255
Asn Val Asn His Asp Ala Gln Gln Trp Leu Tyr Asp Phe Thr Gly Gly
260 265 270
Gly His Ile Lys Ala Asn Asn Pro Ala His Ile Glu Ala Met Asn Lys
275 280 285
Ile Ala Asp Leu Thr Lys Gln Thr Ser Asp Asn Glu Ala Phe Leu Leu
290 295 300
Ala Ser Asn Pro Pro Ser Ala Gly Asp Lys Thr Gly Leu Val Lys Asp
305 310 315 320
Gly Asp Lys Trp Asn Tyr Tyr Ser Pro Arg Asp Gly Phe Glu Asn Thr
325 330 335
Gln His Gln Lys Phe Asn Lys Gly Ser Leu Val Thr Gly Pro Leu Asp
340 345 350
Ile Asp Gly Arg Thr Tyr Leu Phe Ser Phe Glu Glu Gly Met Lys Asn
355 360 365
Ser Ala Gly Lys Lys Phe Asn Lys Gly Asp Met Phe Thr Gly Trp Tyr
370 375 380
Asn Asp Ala Gly Val Trp Ser Tyr Tyr Lys Pro Glu Ser Gly Val Met
385 390 395 400
Ala Thr Gly Trp Gln Asn Ile Ser Gly Lys Trp Tyr Tyr Phe Ala Thr
405 410 415
Ala Tyr Gln Glu Phe Asn Gly Gly Ala Phe Ile Ser Lys Gly Asp Met
420 425 430
Ile Lys Gly Lys Thr Val Glu Ile Ser Gly Lys Leu Tyr Thr Phe Asp
435 440 445
Glu Asn Gly Ala Cys Leu Asn Pro Asp Gly Lys Pro Ile Ala Lys
450 455 460
<210> 332
<211> 375
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 332
Met Ser Asn Glu Ile Lys Asn Asn Glu Asp Met Asn Lys Pro Met Ile
1 5 10 15
Asn Asn Glu Asp Ser Ile Gln Ser Glu Ala Lys Ala Ser Lys Gly Leu
20 25 30
Ile Ala Phe Pro Asp Phe Asn Tyr Tyr Asn Ile Arg Thr Phe Val Gly
35 40 45
Lys Tyr Ala Asp Val Thr Gly Gly Ala Gly Asn Gly Arg Pro Val Val
50 55 60
Gln Tyr His Pro Asn Gly Ser Asp Asn Gln Lys Phe Leu Ile Phe Thr
65 70 75 80
Leu Asp Asn Gly Tyr Ser Val Ile Ala Val Lys His Ser Gly Lys Val
85 90 95
Leu Asp Leu Ala Tyr Asn Ser Ser Gly Asp Arg Leu Ile Gln Tyr Asp
100 105 110
Phe Asn Asn Gly Asp Ile Gln Lys Phe Ala Ile Glu Asn Asn Gly Ser
115 120 125
Ile Arg Val Lys Ile Asn Asn Gln Val Trp Asp Val Tyr Arg Gly Glu
130 135 140
Thr Trp Asp Asn Ala Pro Ile Val Gly Tyr His Tyr Gln Gly Ser Gln
145 150 155 160
Asn Gln Lys Phe Ser Leu Val Lys Asn Gly Val Val Ser Ile Thr Arg
165 170 175
Pro Gln Met Gly Thr Leu Pro Pro Ala Pro Asp Phe Lys Thr Asn Asp
180 185 190
Leu Asn Glu Val Leu Pro Glu Lys Thr Asp Pro Val Asn Thr Lys Val
195 200 205
Thr Phe Ile Pro Tyr Phe Met Ile Lys Asp Pro Ser Tyr Asn His Gln
210 215 220
Gln Gln Met Gln Leu Ser Pro Tyr Tyr Gln Leu Val Arg Arg Gln Tyr
225 230 235 240
Trp Glu Lys Lys Thr Gln Arg Ile Leu Gly Pro Ser Asp Lys Tyr Glu
245 250 255
Tyr Ser Gln Thr Val Gly Val Ser Arg Thr Asp Gln Thr Ser Met Thr
260 265 270
Lys Thr Thr Glu Ile Ser Ile Gly Leu Asp Leu Gly Phe Met Phe Lys
275 280 285
Gly Phe Ser Ala Gly Leu Ser Thr Ser Ile Thr Glu Thr Leu Ser Val
290 295 300
Thr Lys Ser Thr Ser Asn Thr Glu Ser Thr Ala Thr Thr Gln Ile Val
305 310 315 320
Thr Tyr Ser Asn Pro Phe Thr Tyr Ser Ile Ala Arg Ala Asn Tyr Met
325 330 335
Leu Ile Asp Glu Tyr Tyr Ile Thr Arg Ala Asn Gly Glu Lys Ile Val
340 345 350
Val Asp Asn Ala Thr Tyr Trp Arg Val Pro Asn Pro Glu Arg Thr Val
355 360 365
Ala Arg Ile Ile Pro Arg Asn
370 375
<210> 333
<211> 514
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 333
Met Glu Glu Lys Ala Thr Glu Ala Gln Gln Ala Ala Met Asp Ser Leu
1 5 10 15
Gly Val Gly Gly Gly Ala Ser Thr Asp Asp Ala Phe Lys Ala Ala Gly
20 25 30
Glu Ala Leu Lys Glu Val Ala Lys Leu Lys Lys Asp Gly Lys Asn Lys
35 40 45
Phe Thr Gln Glu Ala Gln Asp Thr Ile Thr Leu Leu Gly Lys Ala Ala
50 55 60
Pro Ile Met Tyr Ala Asp Ala His Ala Ser Arg Glu Ser Phe Asn Asn
65 70 75 80
Thr Leu Arg Thr Leu Ser Leu Gly Val Leu Asp Tyr Phe Pro Tyr Gly
85 90 95
Lys Ile Ala Ile Ser Pro Ile Leu Gly Leu Leu Trp Pro Glu Ile Gly
100 105 110
Asn Lys Asn Lys Asn Gln Phe Asp Ser Leu Met Lys Asp Met Lys Ile
115 120 125
Leu Val Lys Glu Gly Ile Glu Thr Tyr Asp Leu Ser Gly Leu Lys Gln
130 135 140
Asn Ile Ile Ser Leu Lys Thr Gln Met Gln Thr Phe Glu Lys Leu Val
145 150 155 160
Asn Ser Asn Thr Leu Thr Glu Gly Leu Tyr Ser Ala Gly Ser Asp Glu
165 170 175
Glu Ser Ala Arg Thr Ala Ala Gln Ile Leu Gln Asp Thr Phe His Asn
180 185 190
Leu Ile Asn Asp Cys Gln Lys Asp Asn Leu Asp Thr Ala Glu Leu Pro
195 200 205
Lys Tyr Thr Thr Ile Ala Thr Ile His Leu Leu Phe Leu Asn Tyr Met
210 215 220
Glu Lys Asn Trp Ser Ser Pro Gln Leu Gln Tyr Pro Lys Ser Thr Phe
225 230 235 240
Asp Lys Ile Phe Ala Asp Asp Ile Lys Asn Ala Ala Gln Thr Tyr Met
245 250 255
Asp Tyr Ile Gln Lys Thr Tyr Thr Ser Gly Asn Ser Gln Phe Val Ser
260 265 270
Lys Leu Lys Asp Val Val Gly Tyr Glu Ser Ser Phe Pro Gln Gln Thr
275 280 285
Gln Arg Asp Leu Phe Lys Lys Tyr Ser Asp Leu Ser Lys Thr Val Ala
290 295 300
Ala Met Glu Ser Gly Lys Asp Asn Thr His Thr Pro Ala Val Ser Leu
305 310 315 320
His Thr Met Lys Glu Arg Leu Asn Glu Ala Glu Lys Asn Tyr Asn Asp
325 330 335
Tyr Lys Asn Leu Val Thr Ile Gln Asp Asn Tyr Tyr Asn Asn Thr Ile
340 345 350
Gly Asn Lys Ala Phe Asp Gly Ile Leu Arg Ala Val Leu Gly Thr Trp
355 360 365
Lys Lys Ala Glu Asn Gly Lys Trp Ile Phe Val Asp Ser Ala Gly His
370 375 380
Lys Lys Ile Asp Trp Leu His Phe Thr Thr Thr Tyr Phe Tyr Leu Ala
385 390 395 400
Pro Ser Gly Gly Thr Glu Asn Ser Asp His Val Lys Phe Thr Glu Gly
405 410 415
Glu Met Val Thr Asp Trp Val Lys Ile Arg Ala Gln Trp Tyr Tyr Phe
420 425 430
Ser Pro Ser Glu Gly Thr Lys Asn Ser Gly Gln Lys Lys Phe Ala Glu
435 440 445
Gly Glu Met Met Thr Gly Trp Val Glu Ile Ala Asp Lys Lys Pro Asn
450 455 460
Asp Arg Pro Gly Glu Arg Thr Gly Thr Lys His Trp Tyr Tyr Leu Asn
465 470 475 480
Pro Glu Asn Gly Ser Met Ile Gln Asp Lys Lys Glu Val Gln Ile Asp
485 490 495
Glu Lys Lys Tyr Asp Phe Asp Ser Asn Gly Val Cys Ile Thr Pro Lys
500 505 510
Gly Tyr
<210> 334
<211> 359
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 334
Met Ala Thr Pro Glu Lys Asn Gly Tyr Tyr Ser Ile His Leu Ala Ser
1 5 10 15
Asn Thr Trp Lys Lys Trp Asp Val Ala Val Ala Ser Leu Asp Asn Gly
20 25 30
Ala Gly Ile Leu Leu Tyr Asp Val Ser Gly Lys Ile Pro Asp Asn Gln
35 40 45
Gln Phe Ala Phe Phe Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn
50 55 60
Lys Asn Ser Gly Lys Pro Val Thr Ile Gly Asp Gly Val Trp Pro Met
65 70 75 80
Asp Gly Leu Gly Gly Asn Ser Gly Ala Leu Lys Gln Asn Asn Trp Thr
85 90 95
Gly Asn Ala Asn Glu Lys Trp Tyr Leu Arg Asp Leu Gly Gly Asn Asp
100 105 110
Phe Glu Ile Val Asn Gln Gly Thr Gly Lys Val Ala Ser Tyr Ala Trp
115 120 125
Val Gly Asp Leu Val Arg His Phe Glu Tyr Val Asp Leu Asp Asn Ala
130 135 140
Asn Pro Ser Asp Arg Asp Arg Val Phe His Ile Pro Ala Ala Arg Ser
145 150 155 160
Thr Leu Thr Val Pro Thr Leu Pro Ala Thr Gly Thr Arg Pro Val Ala
165 170 175
Pro Asn Tyr Thr Gly Gly Ile Asp Glu Gln Leu Pro Gln Thr Ser Lys
180 185 190
Ser Val Ile Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Lys Asp
195 200 205
Ser Gln Thr Asn Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr
210 215 220
Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln
225 230 235 240
Pro Gly Leu Ser Glu Thr Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val
245 250 255
Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ala Asp
260 265 270
Phe Gly Ile Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile
275 280 285
Ser Arg Thr Ile Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr
290 295 300
Glu Glu Thr Arg Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr
305 310 315 320
Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala
325 330 335
Asp Gly Ser Ile Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile
340 345 350
Thr Val Thr Arg Lys Thr Leu
355
<210> 335
<211> 369
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 335
Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Ser Gly Asn Gln Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Tyr Leu Glu Asn Ala Pro Ser Glu Arg Phe Phe
35 40 45
Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Ile Ser Gly Asn Gln Thr Gly Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Phe Glu Trp Leu Gly Asp Ala Pro Ser Glu Tyr Trp Tyr
85 90 95
Phe His Arg Glu Ala Asp Gly His Tyr Val Ile Glu Ser Lys Leu Ser
100 105 110
Gly Lys Val Leu Asp Ile Ala Gly Asn Ser Thr Ala Asn Asn Ala Asn
115 120 125
Val Gln Gln Phe Glu Phe Leu Ala Asp Ala Pro Ser Glu Arg Phe Ala
130 135 140
Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile Asn Thr Gln Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp Gln Leu Pro Glu
165 170 175
Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val Pro Ala Ile Ser
180 185 190
Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Glu
210 215 220
Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr Thr Ser Lys Thr
225 230 235 240
Gly Ile Lys Val Thr Asp Gln Glu Thr Ala Thr Lys Thr Val Ser Trp
245 250 255
Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly Phe Ser Val Gly
260 265 270
Met Ser Ser Gln Tyr Ser Thr Gln Leu Gln Thr Ser Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu Ile Lys Asn Pro
290 295 300
Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu Thr Thr Glu Tyr
305 310 315 320
Ser Val Gln Arg Lys Ser Gly Thr Ile Val Asn Ser Pro Trp Thr Met
325 330 335
Thr Asp Lys Thr Arg Thr His Ala Val Thr Tyr Pro Asn Ala Glu Glu
340 345 350
Lys Ala Leu Asn Glu Asn Thr Lys Gln Leu Ser Lys Ala Glu Ser Val
355 360 365
Asn
<210> 336
<211> 373
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 336
Met Gly Lys Ser Met Ile Phe Lys Val Gly Met Lys Tyr Arg Phe Arg
1 5 10 15
Asn Lys Asn Ser Gly Lys Tyr Leu Asp Ile Ala Gly Asn Gln Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Leu Val Lys Asp Leu Pro Ser
35 40 45
Glu Gln Phe Phe Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Ile Ile
50 55 60
Asn Val Asn Ser Gly Lys Val Leu Asp Ile Ala Gly Asn Gln Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Phe Glu Trp Arg Gly Asp Leu Pro Ser
85 90 95
Glu Tyr Trp Tyr Phe His Leu Glu Glu Asp Gly Tyr Tyr Val Ile Glu
100 105 110
Ser Lys His Ser Gly Lys Val Leu Asp Ile Ala Gly Asn Gln Thr Gly
115 120 125
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Ile Lys Asn Leu Pro Ser
130 135 140
Glu Arg Phe Ala Ala Glu Glu Val Glu Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Ser Thr Gln Pro Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Lys Glu Thr Glu His Val Val Thr Ala Phe Thr Ile Val
180 185 190
Pro Ala Ile Ser Val Arg Asp Pro Leu Tyr Gly Gly Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr
225 230 235 240
Thr Ser Lys Thr Gly Met Lys Val Thr Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Leu Ser Ile Gly Val Asp Met Gly Phe Asn Phe Lys Gly
260 265 270
Val Ser Val Gly Met Ser Thr Gln Tyr Ser Thr Gln Leu Gln Ala Ser
275 280 285
Thr Ser His Thr Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu
290 295 300
Ile Lys Asn Pro Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Ser Val Glu Arg Lys Asn Gly Thr Ile Val Asn Ser
325 330 335
Pro Trp Thr Met Thr Asp Lys Thr Ser Ala His Ser Val Thr Tyr Pro
340 345 350
Asn Ala Glu Glu Lys Ala Leu Asn Glu Asn Thr Lys Gln Leu Ser Lys
355 360 365
Val Glu Ser Val Asn
370
<210> 337
<211> 369
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 337
Met Ile Phe Lys Val Gly Met Lys Tyr Arg Phe Arg Asn Lys Asn Ser
1 5 10 15
Gly Lys Tyr Leu Asp Ile Ala Gly Asn Gln Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Leu Val Lys Asp Leu Pro Ser Glu Gln Phe Phe
35 40 45
Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Ile Ile Asn Val Asn Ser
50 55 60
Gly Lys Val Leu Asp Ile Ala Gly Asn Gln Thr Gly Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Phe Glu Trp Arg Gly Asp Leu Pro Ser Glu Tyr Trp Tyr
85 90 95
Phe His Leu Glu Glu Asp Gly Tyr Tyr Val Ile Glu Ser Lys His Ser
100 105 110
Gly Lys Val Leu Asp Ile Ala Gly Asn Gln Thr Gly Asn Asn Ala Asn
115 120 125
Val Gln Gln Tyr Glu Tyr Ile Lys Asn Leu Pro Ser Glu Arg Phe Ala
130 135 140
Ala Glu Glu Val Glu Ser Val Ser Leu Pro Ser Ile Ser Thr Gln Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp Gln Leu Pro Lys
165 170 175
Glu Thr Glu His Val Val Thr Ala Phe Thr Ile Val Pro Ala Ile Ser
180 185 190
Val Arg Asp Pro Leu Tyr Gly Gly Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Glu
210 215 220
Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Lys Tyr Thr Ser Lys Thr
225 230 235 240
Gly Met Lys Val Thr Asp Gln Glu Thr Ala Thr Lys Thr Val Ser Leu
245 250 255
Ser Ile Gly Val Asp Met Gly Phe Asn Phe Lys Gly Val Ser Val Gly
260 265 270
Met Ser Thr Gln Tyr Ser Thr Gln Leu Gln Ala Ser Thr Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu Ile Lys Asn Pro
290 295 300
Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu Thr Thr Glu Tyr
305 310 315 320
Ser Val Glu Arg Lys Asn Gly Thr Ile Val Asn Ser Pro Trp Thr Met
325 330 335
Thr Asp Lys Thr Ser Ala His Ser Val Thr Tyr Pro Asn Ala Glu Glu
340 345 350
Lys Ala Leu Asn Glu Asn Thr Lys Gln Leu Ser Lys Val Glu Ser Val
355 360 365
Asn
<210> 338
<211> 370
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 338
Met Ala Ser Pro Glu Asn Asn Arg Tyr Tyr Ser Val His Leu Lys Ala
1 5 10 15
Asn Arg Asp Lys Val Trp Asp Val Tyr Arg Asn Asn Thr Tyr Asp Asn
20 25 30
Ala Gly Ile Leu Leu Tyr Gly Gln Asn Gly Gln Asp Asn Gln Lys Phe
35 40 45
Val Phe Phe Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn
50 55 60
Ser Gly Lys Pro Val Gly Ile Gly Asp Pro Ile Ala Asn Asp Asp Gly
65 70 75 80
Val Arg Arg Met Thr Leu Phe Asp Asn Glu Gly Leu Arg Gln Glu Ser
85 90 95
Trp Thr Gly Ala Thr Asp Gln Gln Trp Tyr Leu Arg Asp Gln Gly Gly
100 105 110
Ser Asn Tyr Glu Ile Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr
115 120 125
Ala Trp Glu Gly Thr Leu Ala Gln Asn Trp Glu Tyr Thr Asp Leu Asp
130 135 140
Glu Ser Asn Pro Ser Asp Ser Asp Arg Val Phe Tyr Ile Ser Ala Gly
145 150 155 160
Pro Val Asn Gln Gly Thr Gln Pro Leu Tyr Gly Thr Phe Ser Leu Pro
165 170 175
Lys Leu Pro Ala Val Gly Asn Arg Pro Asp Ala Pro Asn Tyr Thr Gly
180 185 190
Gly Ile Glu Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly
195 200 205
Ala Ser Leu Ile Pro Cys Phe Met Val Asn Asp Gly Gln Ala Asp Asn
210 215 220
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Val Leu Gln Lys Glu Glu
225 230 235 240
Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu
245 250 255
Ser Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met
260 265 270
Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Leu Gly Leu Lys Phe
275 280 285
Gly Gln Gln Thr Ala Ser Ile Lys Ser Glu Ile Ser Arg Thr Ile Gln
290 295 300
Thr Glu Val Ser Thr Thr Asn Thr Glu Ala Thr Glu Glu Thr Arg Ala
305 310 315 320
Tyr Thr Ala Val Gly Glu Ala Gly Arg Ala Thr Gly Tyr Thr Gln Tyr
325 330 335
Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val
340 345 350
Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys
355 360 365
Thr Ser
370
<210> 339
<211> 515
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 339
Met Ala Glu Asn Thr Thr Glu Lys Gln Lys Val Ser Ser Tyr Pro Lys
1 5 10 15
Asn Ser Asn Thr Val Gly Ser Asn Val Phe Lys Val Ala Ala Asp Gly
20 25 30
Ser Val Thr Leu Leu Asn Ser Thr Pro Phe Asp Ser Pro Thr Val Lys
35 40 45
Ala Ile Thr Leu Leu Gly Gly Asn Leu Leu Asn Gln Ala Tyr Leu Asp
50 55 60
Ala Tyr Asn Lys Asp Phe Thr Gly Thr Ala Arg Thr Leu Val Leu Gly
65 70 75 80
Ala Thr Ala Leu Ile Pro Tyr Val Gly Ser Phe Ile Ser Pro Ile Ile
85 90 95
Gly Leu Leu Trp Pro Ala Asn Val Gly Thr Asp Met Thr Ala Gln Thr
100 105 110
Lys Ala Leu Val Ala Leu Ile Asn Glu Gln Ile Ala Asp Tyr Asp Ile
115 120 125
Glu Thr Leu Glu Gln Lys Gly Lys Thr Leu Lys Asp Leu Met Ser Arg
130 135 140
Phe Asp Asp Leu Leu Asn Gly Lys Pro Val Thr Phe Gly Glu Gln Gly
145 150 155 160
Thr Val Gln Glu Thr Leu Arg Asn Lys Ala Ser Asn Ile Asn Asp Lys
165 170 175
Phe Ile Glu Val Ile Asn Asp Cys Gln Lys Arg Ser Phe Lys Ala Glu
180 185 190
Glu Leu Pro Ile Tyr Thr Leu Ile Ala Thr Ala His Leu Leu Phe Leu
195 200 205
Asn Met Ile Glu Gln Asn Gly Gln Ser Ser Arg Ile Gln Met Asp Asp
210 215 220
Glu Thr Tyr Lys Gly Tyr Val Glu Asp Phe Lys Arg Leu Pro Ile Glu
225 230 235 240
Tyr Ala Asn Tyr Ile Glu Ala Thr Ser Lys Leu Gly Lys Gln Lys Phe
245 250 255
Asn Lys Lys Met Asp Asp Thr Ile His Ala Ala Lys Lys Gln Ala Leu
260 265 270
Val Gly Ile Ile Ser Ser Asp Glu Asn Thr Asn Leu Glu Asn Leu Glu
275 280 285
Lys Thr Arg Lys Gln Cys Leu Asp Glu Ala Lys Leu Leu Gln Gln Ile
290 295 300
Gly Glu Val Tyr Gly Ala Glu Lys Ala Arg Gln Lys Ala Gln Lys Ala
305 310 315 320
Tyr Glu Tyr Lys Lys Gln Tyr Glu Asp Leu Ile Gln Lys Arg Gly Asn
325 330 335
Tyr Ile Gln Asn Thr Ala Asp Asn Glu Ala Phe Lys Leu Ala Lys Lys
340 345 350
Ser Met Glu Glu Leu Met Ile Asp Val Arg Ile Pro Asn Gly Lys Tyr
355 360 365
Ile Ile Gln Ser Thr Gln Asn Ser Ser Met Val Val Gly Met Ser Gly
370 375 380
Asn Asn Ile Gln Leu Glu Glu Asn Asn Gly Gln Leu Asn Asn Lys Phe
385 390 395 400
Phe Ile Ile Tyr Asp Gln Ala Arg Asn Ser Tyr Val Ile Glu Thr Ser
405 410 415
Ile Glu Thr Gly Leu Ser Ser Tyr Met Ala Ala Asn Ile Asp Tyr Ser
420 425 430
Asn Val Asn Lys Ser Gly Val Asn Val Tyr Ala Ile Leu Ser Thr Ser
435 440 445
Ser His Glu Tyr Pro Asp Ile Leu His Trp Lys Leu Lys Arg Glu Arg
450 455 460
Gly Ser Lys Phe Val Tyr Leu Glu Asn Leu Ala Asn Gly Gln Val Leu
465 470 475 480
Asp Val Ser Asn Ala Gly Thr Glu Lys Gly Thr Asn Ile Ile Thr Trp
485 490 495
Pro Leu Lys Gln Gln Gly Gln Asn Gln Asn Gln Gly Phe Glu Leu Val
500 505 510
Pro Thr Asn
515
<210> 340
<211> 371
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 340
Met Ala Ser Pro Glu Asn Asn Arg Tyr Tyr Ser Ile His Leu Glu Ser
1 5 10 15
Asn Lys Asn Lys Val Trp Asp Val Phe Asn Asn Ala Thr Tyr Asp Asn
20 25 30
Ala Gly Ile Leu Leu Tyr Gly Gln Thr Asn Ser Asp Asn Gln Lys Phe
35 40 45
Ala Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn
50 55 60
Ser Gly Lys Pro Val Gly Ile Gly Asp Pro Ile Ile Ile Asp Asp Gly
65 70 75 80
Val Arg Arg Met Thr Ala Phe Asn Asn Glu Gly Leu Arg Gln Thr Ser
85 90 95
Trp Thr Gly Ala Ser Ala Glu Lys Trp Tyr Leu Arg Glu Lys Ser Pro
100 105 110
Ser Asn Tyr Glu Ile Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr
115 120 125
Ala Trp Gln Gly Thr Leu Ala Gln Asn Trp Glu Tyr Ala Asp Leu Asp
130 135 140
Asn Ser Asn Pro Thr Asp Gly Asp Arg Val Phe Tyr Val Ser Ala Gly
145 150 155 160
Gln Val Asn Gln Gly Asn Val Pro Phe His Gly Thr Phe Ala Ile Pro
165 170 175
Gln Leu Pro Ala Thr Gly Asn Arg Pro Asp Ala Pro Asn Tyr Thr Gly
180 185 190
Gly Val Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly
195 200 205
Ala Ser Leu Ile Pro Cys Phe Met Val Asn Asp Asn Gln Ala Ser Asp
210 215 220
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu
225 230 235 240
Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Leu Thr Arg
245 250 255
Asn Tyr Ser Phe Lys Thr Gly Met Thr Ser Val Asp Gln Gln Lys Met
260 265 270
Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Leu Gly Leu Lys Phe
275 280 285
Gly Glu Gln Ser Ala Ser Ile Lys Ser Glu Val Ser Arg Thr Leu Gln
290 295 300
Thr Glu Ile Ser Thr Thr Asn Thr Glu Ala Thr Glu Glu Thr Val Thr
305 310 315 320
Ser Thr Val Glu Ser Glu Ala Gly Lys Thr Thr Gly Phe Thr Glu Tyr
325 330 335
Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val
340 345 350
Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile Ala Arg Lys
355 360 365
Asn Ala Gln
370
<210> 341
<211> 535
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 341
Met Ala Glu Asp Thr Gly Asn Val Ser Phe Leu His Pro Lys Lys Glu
1 5 10 15
Pro Ala Lys Lys Glu Asp Pro Met Asp Ile Leu Asn Trp Asn Lys Tyr
20 25 30
Glu Asp Val Phe Asn Lys Asn Arg Gly Asn Ser Ile Ala Lys Leu Thr
35 40 45
Gly Ala Gly Leu Lys Glu Ile Phe Ser Gln Gly Asn Lys Ser Ala Pro
50 55 60
Asp Phe Asn Gln Leu Ala Lys Thr Leu Thr Met Thr Ala Ile Asp Leu
65 70 75 80
Phe Pro Gly Gly Met Phe Ile Thr Pro Leu Leu Asn Leu Phe Trp Pro
85 90 95
Ser Ala Gln Gly Ser Asn Gly Asn Glu Thr Leu Ile Lys Asn Leu Thr
100 105 110
Glu Lys Leu Thr Lys Val Met Asp Glu Lys Ile Asp Ala Glu His Leu
115 120 125
Asn Gln Met Thr Asn Asp Phe Lys Ala Leu Ile Glu Asp Ile Thr Arg
130 135 140
Leu Gln Asn Phe Leu Asn Asn His Ser Lys Phe Val Leu Pro Asp Met
145 150 155 160
Ser Phe Thr Ala Phe Val Asp Val Asp Asp Ser Gln Leu Tyr Ser Gly
165 170 175
Thr Asn Leu Thr Ser Ala Gly Ala Val Ala Leu Asn Ile Glu Ser Ala
180 185 190
Phe Thr Arg Ile Leu Thr Gln Val Ser Asp Glu Lys His Asp Thr Thr
195 200 205
Asp Leu Met Leu Tyr Thr Asn Val Ala Asn Ile His Leu Gln Phe Leu
210 215 220
Lys Tyr Ile Glu Lys Tyr Gly Ala Ser Glu Lys Phe Gly Leu Ser Lys
225 230 235 240
Leu Asp Leu Glu Lys Phe Phe Lys Ser Asp Thr Ile Ser Ser Ile Thr
245 250 255
Glu Lys Tyr Ala Lys His Ile Glu Asp Thr His Ser Lys Ala Met Asp
260 265 270
Lys Leu Asn Lys Arg Ile Glu Asp Leu Gly Gly Asn Glu Glu Thr Leu
275 280 285
Asn Asn Lys Ile Lys Glu Val Glu Asn Ser Arg Ala Gln Arg Ser Ile
290 295 300
Asp Pro Asp Ala Ala Ile Leu Gln Glu Lys Glu His Glu Lys Trp Lys
305 310 315 320
Lys Glu Glu Val Thr Lys Leu Lys Ala Asn Ile Glu Lys Val Lys Glu
325 330 335
Ile Glu Thr Glu Lys Gln Asn Leu Thr Glu Leu Thr Ile Lys Asn Glu
340 345 350
Gly Phe Lys Ala Ala Ser Gly Lys Thr Leu Asn Val Leu Glu Ser Gly
355 360 365
Phe Phe Lys Gln Asn Gly Lys Thr Tyr Tyr Arg Gly Tyr Arg Gly Asp
370 375 380
Glu Thr Lys Val Glu Asn Gly Thr Leu Ala Thr Gly Trp Gln Thr Ile
385 390 395 400
Lys Gly Lys Thr Tyr Tyr Phe Gly Ala Pro Gly Asp Asn Thr Lys Val
405 410 415
Glu Asn Asn Thr Ala Ala Thr Gly Trp Gln Thr Ile Lys Gly Lys Thr
420 425 430
Tyr Tyr Phe Gly Ala Pro Gly Asp Asn Thr Lys Val Glu Asn Asn Thr
435 440 445
Ala Ala Thr Gly Leu Gln Lys Ile Gly Gly Lys Thr Tyr Tyr Phe Gly
450 455 460
Lys Pro Gly Asp Gly Thr Thr Val Asp Asn Tyr Thr Met Ala Thr Gly
465 470 475 480
Trp Val Val Leu Asp Asn Gly His Asp Trp Tyr Tyr Cys Ala Pro Ser
485 490 495
Asp Leu Thr Thr Asp Asp Gly Lys His Phe Ser Lys Gly Glu Leu Leu
500 505 510
Thr Asn Thr Glu Phe Thr Ile Asn Gly Lys Thr His Val Phe Pro Glu
515 520 525
Ser Gly Arg Ala Tyr Ala Lys
530 535
<210> 342
<211> 528
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 342
Met Ala Glu Asn Thr Lys Asp Thr Gln Lys Gln Ser Gln Asn Val Val
1 5 10 15
Ser Lys Val Met Ser Asn Thr Gly Asp Lys Ala Phe Glu Ile Leu Asn
20 25 30
Asp Gly Thr Ile Lys Val Ile Asn Glu Gln Tyr Phe Pro Lys Gly Asn
35 40 45
Glu Glu Trp Phe Lys Lys Thr Leu Val Lys Met Gly Gly Gln Thr Phe
50 55 60
Leu Asp Thr Met Lys Ser Leu Asn Gly Asn Gly Ala Met Ser Ile Asn
65 70 75 80
Asn Phe Ala Lys Asp Met Val Val Val Ser Thr Ser Leu Ile Pro Tyr
85 90 95
Ala Gly Ala Ile Ile Ser Pro Leu Ile Asn Leu Leu Trp Pro Ser Ser
100 105 110
Val Ser Asp Ser Gln Asn Gln Ile Gln Leu Leu Met Lys Met Met Asp
115 120 125
Asn Lys Ile Ser Asp Tyr Asp Ile Asn Ser Leu Lys Thr Asp Val Lys
130 135 140
Ala Ala Val Glu Glu Leu Asn Lys Leu Glu Val Gly Leu Gln Gly Ala
145 150 155 160
Asn Gly Phe Lys Ala Tyr Arg Thr Ser Ala Asp Tyr Asn Ala Gly Gln
165 170 175
Ala Glu Ala Val Asn Arg Ala Phe Arg Lys Ile Met Gln Asn Ser Leu
180 185 190
Lys Glu Gly Leu Glu Lys Ser Glu Leu Pro Ile Tyr Thr Met Val Ala
195 200 205
Thr Ala Lys Leu Ile Phe Leu Ser Tyr Met Val Glu Asn Gly Gly Asn
210 215 220
Ala Asp Phe Lys Tyr Asp Asp Ala Thr Leu Lys Asn Ala Tyr Ile Asn
225 230 235 240
Ser Asp Leu Ala Val Asp Ile Asn Asn Phe Glu Glu His Ile Gln Lys
245 250 255
Ile Ser Ala Glu Ser Val Glu Arg Leu Asn Thr Leu Asn Thr Glu Val
260 265 270
Pro Lys Ala Val Asp Gln Met Ser Ala Leu Gln Thr Lys Ile Asn Lys
275 280 285
Leu Gln Asp Ala Ala Asn Lys Gly Asn Pro Glu Ala Ala Ala Ala Leu
290 295 300
Ala Gly Ala Val Glu Leu Leu Asn Lys Leu His Thr Gln Tyr Asp Pro
305 310 315 320
Met Ile Lys Glu Gln Lys Lys Leu Gln Asp Tyr Val Ser Ser Thr Ala
325 330 335
Lys Asn Asp Ala Phe Lys Val Ala Leu Glu Thr Ala Lys Asn Thr Tyr
340 345 350
Lys Lys His Gly Gly Gln Ile Lys Glu Lys Asp Gly Phe His Gln Glu
355 360 365
Gly Asn Asn Trp Tyr Tyr Thr Lys Asn Gly Glu Lys Gln Lys Gly Trp
370 375 380
Ile Lys Asp Asn Gly Lys Asp Tyr Tyr Leu Ser Lys Glu Asp Gly His
385 390 395 400
Met Lys Thr Gly Trp Val Gln Phe Glu Lys Asp Asn Glu Arg Asp Tyr
405 410 415
Asn Pro Ala Ala Val Asn Gln Asp Asn Val Thr Thr Tyr Tyr Leu Asp
420 425 430
Thr Asp Gly His Met Val Thr Gly Trp Lys Asn Ile Gly Gly Tyr Tyr
435 440 445
Tyr Tyr Phe Asn Lys Pro Glu Asn Thr Gly Glu Asn Gly His Tyr Phe
450 455 460
Lys Asn Gly Glu Met Ile Lys Gly Trp Ile Arg Leu Ser Glu Asp Gly
465 470 475 480
Ser Glu Lys Glu Val Ala Asp Gly Gly Tyr Trp Tyr Tyr Leu Gly Arg
485 490 495
Asp Gly Ser Met Leu Lys Asp Gly Thr Tyr Leu Thr Phe Asp Arg Ala
500 505 510
Gly Thr Ser Lys Gln Ala His Phe Asp Lys Tyr Gly Arg Tyr Thr Gly
515 520 525
<210> 343
<211> 407
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 343
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Ala Val Gly Ala
1 5 10 15
Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Glu Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Ser Ile His Leu Lys Ala Asn Pro Lys Leu Ala
35 40 45
Trp Asp Val Tyr Lys Gly Trp Asp Ser Asp Asn Ala Gly Ile Ile Leu
50 55 60
Tyr Asn Gly Ser His Gly Asp Asn Glu Gln Phe Ala Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Phe Gly Gly Gln Ala Tyr Tyr Trp Thr Asp Gly Val Arg Asp Gly
100 105 110
Phe Val Met Asn Arg Asp Ala Leu Gln Gln Lys Asn Trp Thr Gly Ser
115 120 125
Ala Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu
130 135 140
Ile Val Asn Gln Gly Thr Gly Lys Val Val Ser Trp Ala Gly Thr Pro
145 150 155 160
Val Thr Gly Gly Tyr Leu Asp Tyr Val Asp Leu Asp Glu Ser Asn Pro
165 170 175
Ser Asp Asn Asn Lys Val Phe Asn Ile Ala Lys Asn Gly Phe Gly Gly
180 185 190
Ile Gly Val Ser Ile Pro Gly Phe Asp Phe Asp Tyr Gly Thr Phe Ser
195 200 205
Leu Pro Thr Leu Pro Ala Val Gly Thr Arg Pro Asn Ala Pro Asp Tyr
210 215 220
Asn Thr Ser Gly Ala Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser
225 230 235 240
Val Val Val Gly Ala Ser Leu Ile Pro Ser Ile Met Val Lys Asp Asn
245 250 255
Gly Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Val Leu
260 265 270
Val Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu Ile Ile Pro Ala
275 280 285
Gly Ala Thr Ser Lys Tyr Thr Val Lys Ser Gly Val Ser Glu Ile Asp
290 295 300
Gln Lys Lys Met Ser Asp Thr Leu Ala Met Ser Phe Gly Ser Asp Leu
305 310 315 320
Gly Leu Lys Phe Gly Glu Ser Ser Leu Ser Ile Lys Ser Ser Val Ser
325 330 335
Arg Thr Ile Gln Thr Glu Val Thr Lys Thr Ser Thr Asn Ala Thr Glu
340 345 350
Glu Thr Glu Glu Lys Ser Ile Pro Ser Ile Pro Gly Lys Asn Thr Gly
355 360 365
Phe Thr Ala Tyr Gln Leu Val Thr Lys Tyr Thr Leu Lys Arg Thr Asp
370 375 380
Gly Ser Gly Val Thr Asp Pro Ser Ile Val Arg Asp Ser Lys Gln Thr
385 390 395 400
Ile Ala Arg Thr Ile Ala Asn
405
<210> 344
<211> 379
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 344
Met Ala Pro Glu Asn Asn Arg Tyr Tyr Ser Ile His Leu Lys Ala Asn
1 5 10 15
Pro Lys Leu Ala Trp Asp Val Tyr Lys Gly Trp Asp Ser Asp Asn Ala
20 25 30
Gly Ile Ile Leu Tyr Asn Gly Ser His Gly Asp Asn Glu Gln Phe Ala
35 40 45
Phe Phe Pro Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser
50 55 60
Gly Lys Pro Val Gly Phe Gly Gly Gln Ala Tyr Tyr Trp Thr Asp Gly
65 70 75 80
Val Arg Asp Gly Phe Val Met Asn Arg Asp Ala Leu Gln Gln Lys Asn
85 90 95
Trp Thr Gly Ser Ala Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn
100 105 110
Asn Asn Tyr Glu Ile Val Asn Gln Gly Thr Gly Lys Val Val Ser Trp
115 120 125
Ala Gly Thr Pro Val Thr Gly Gly Tyr Leu Asp Tyr Val Asp Leu Asp
130 135 140
Glu Ser Asn Pro Ser Asp Asn Asn Lys Val Phe Asn Ile Ala Lys Asn
145 150 155 160
Gly Phe Gly Gly Ile Gly Val Ser Ile Pro Gly Phe Asp Phe Asp Tyr
165 170 175
Gly Thr Phe Ser Leu Pro Thr Leu Pro Ala Val Gly Thr Arg Pro Asn
180 185 190
Ala Pro Asp Tyr Asn Thr Ser Gly Ala Ile Asp Gln Gln Leu Pro Gln
195 200 205
Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro Ser Ile Met
210 215 220
Val Lys Asp Asn Gly Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro
225 230 235 240
Tyr Tyr Val Leu Val Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu
245 250 255
Ile Ile Pro Ala Gly Ala Thr Ser Lys Tyr Thr Val Lys Ser Gly Val
260 265 270
Ser Glu Ile Asp Gln Lys Lys Met Ser Asp Thr Leu Ala Met Ser Phe
275 280 285
Gly Ser Asp Leu Gly Leu Lys Phe Gly Glu Ser Ser Leu Ser Ile Lys
290 295 300
Ser Ser Val Ser Arg Thr Ile Gln Thr Glu Val Thr Lys Thr Ser Thr
305 310 315 320
Asn Ala Thr Glu Glu Thr Glu Glu Lys Ser Ile Pro Ser Ile Pro Gly
325 330 335
Lys Asn Thr Gly Phe Thr Ala Tyr Gln Leu Val Thr Lys Tyr Thr Leu
340 345 350
Lys Arg Thr Asp Gly Ser Gly Val Thr Asp Pro Ser Ile Val Arg Asp
355 360 365
Ser Lys Gln Thr Ile Ala Arg Thr Ile Ala Asn
370 375
<210> 345
<211> 551
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 345
Met Asp Glu Thr Ser Thr Arg Thr Arg Gln Thr Ala Leu Glu Ser Leu
1 5 10 15
Gly Thr Ser Gly Gly Ala Gly Ala Thr Ser Ser Lys Leu Glu Glu Leu
20 25 30
Leu Thr Met Asp Asn Leu Lys Lys Val Gly Thr Leu Ser Gly Asp Val
35 40 45
Leu Lys Val Ala Tyr Lys Asp Ala His Glu Asn Asn Gly Asn Tyr Asn
50 55 60
Asn Thr Phe Arg Thr Leu Ala Met Ser Gly Thr Ala Leu Ile Pro Tyr
65 70 75 80
Gly Gly Val Ile Ile Ser Pro Leu Ile Gly Leu Leu Trp Pro Glu Asn
85 90 95
Val Glu Ala Gln Lys Asn Gln Met Lys Lys Leu Ile Asp Asp Leu Asn
100 105 110
Asn Met Met Asp Gln Lys Ile Thr Asp Asn Glu Ile Ala Thr Ile Glu
115 120 125
Ser Glu Val Asn Ile Leu Lys Glu Met Leu Gln Lys Phe Glu Lys Asn
130 135 140
Ile Asn Met Asn Ile Ser Ala Gly Ser Phe Tyr Ser Leu Thr Thr Lys
145 150 155 160
Glu Gln Ala Leu Ala Gln Asp Ala Lys Asp Ile Asn Asn Lys Phe Ser
165 170 175
Asp Ile Ile Gly His Cys Lys Gln Lys Leu Gln Val Ile Pro Ala Leu
180 185 190
Pro Ile Phe Thr Ala Met Ala Thr Ala His Leu Glu Phe Leu Tyr Phe
195 200 205
Leu Glu Lys Asn Gly Thr Gly Pro Lys Leu Gln Tyr Thr Thr Asp Ala
210 215 220
Leu Asn Thr Leu Leu Lys Gly Glu Asp Asn Glu Asp Phe Gly Lys Arg
225 230 235 240
Ile Lys Asn Leu Ala Glu Thr Tyr Val Asn His Val Gln Lys Thr Tyr
245 250 255
Glu Leu Gly Lys Gln Gln Phe Asn Lys Lys Met Asp Asp Thr Ala Lys
260 265 270
Asn Thr Pro Gly Gly Asn Val Asn Ser Thr Ser Glu Gln Glu Thr Met
275 280 285
Ser Lys Met Gln Lys Tyr Ile Phe Asp Leu His Pro Val Ala Asn Asn
290 295 300
Ala Asp Glu Lys Arg Lys Gln Met Gln Val Ala Leu Asn Asn Tyr Val
305 310 315 320
Lys Leu Arg Asn Asp Arg Glu Gln Tyr Tyr Lys Asn Thr Ala Asn Asn
325 330 335
Glu Val Phe Gln Leu Leu Ala Ile Gly Lys Trp Lys Gln Glu Gly Asp
340 345 350
Lys Trp Tyr Phe Ile Asp Thr Lys Gly Glu Lys Lys Thr Gly Trp Ile
355 360 365
Gln Leu Gly Glu Lys His Tyr Tyr Leu Ser Pro Lys Asn Gly Ile Met
370 375 380
Val Lys Gly Phe Val Thr Ile Glu Gly Lys Pro Tyr Gln Phe Asn Asp
385 390 395 400
Ser Gly Glu Leu Ile Phe Ser Glu Gly Thr Tyr Lys Ile Val Tyr Ser
405 410 415
Lys Ala Asn Lys Val Val Asp Phe Gly Ala Tyr Gly Glu Gln Pro Val
420 425 430
Ile Trp Asp Tyr His Pro Asp Trp Asn Thr Pro Asn Thr Asn Gln Gln
435 440 445
Trp Glu Phe Lys Tyr Asp Ala Ser Lys Asn Ala Tyr Gln Ile Ile Asn
450 455 460
Lys Gln Asn Asn Lys Val Leu Ala Tyr Asn Thr Thr Ser Gly Thr Asp
465 470 475 480
Tyr Thr Ala Leu Val Thr Glu Asn Gln His Lys Ala Glu His Tyr Trp
485 490 495
Thr Leu Glu Asp Ala Val Asp Gly Asn Ile Leu Leu Val Asn Phe Ala
500 505 510
Asn Lys Asn Lys Val Leu Asp Val Tyr Gly Glu Glu Thr Ala Asn Gly
515 520 525
Ser Lys Val Tyr Val Arg Asp Lys Leu Asn Asn Ser Met Ala Gln Lys
530 535 540
Phe Lys Leu Ile Lys Leu Asn
545 550
<210> 346
<211> 376
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 346
Met Ala Ser Leu Glu Lys Asn Arg Tyr Tyr Ser Val His Ser Lys Lys
1 5 10 15
Asp Phe Asn Leu Thr Trp Asp Val Tyr Gly Ser Lys Asp Tyr Asp Asn
20 25 30
Ala Gly Ile Leu Leu Tyr Lys Ser Asn Gly Gln Asp Asn Gln Gln Phe
35 40 45
Val Phe Phe Pro Leu Asp Gly Gly Ser Tyr Ala Ile Val Asn Lys Asn
50 55 60
Ser Gly Lys Pro Val Gly Ile Gly Asp Pro Ile Ala Ile Asp Asp Gly
65 70 75 80
Val Arg Arg Met Thr Leu Phe Asp Asn Glu Gly Leu Leu Gln Lys Gly
85 90 95
Trp Thr Gly Ala Ser Ala Glu Gln Trp Tyr Leu Arg Asp Gln Gly Asn
100 105 110
Asn Asn Tyr Glu Phe Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr
115 120 125
Ala Trp Thr Gly Thr Leu Ala Gln Asn Trp Thr Tyr Val Asp Leu Asp
130 135 140
Asp Ala Asn Pro Ser Asp Asn Asp Arg Val Phe Thr Ile Ser Lys Ser
145 150 155 160
Gly Leu Pro Gly Gly Ile Gln Ile Pro Gly Phe Asn Phe Asp His Gly
165 170 175
Thr Phe Ser Leu Gln Gln Leu Pro Ala Thr Gly Thr Arg Pro Asp Ala
180 185 190
Pro Asn Tyr Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn
195 200 205
Ser Val Val Val Gly Ala Ser Leu Val Pro Cys Ile Met Val Lys Asp
210 215 220
Asn Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr
225 230 235 240
Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro
245 250 255
Ala Gly Leu Thr Arg Asn Tyr Ser Phe Lys Thr Gly Met Thr Ser Val
260 265 270
Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp
275 280 285
Leu Gly Leu Lys Phe Gly Gln Gln Ser Ala Ser Ile Lys Thr Glu Val
290 295 300
Ser Arg Thr Leu Gln Thr Glu Ile Ser Thr Thr Asn Thr Glu Ala Thr
305 310 315 320
Glu Glu Thr Val Thr Ser Thr Val Ser Ser Glu Ala Gly Lys Thr Thr
325 330 335
Gly Phe Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala
340 345 350
Asp Gly Ser Ile Val Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile
355 360 365
Thr Ile Ala Arg Lys Asn Ala Gln
370 375
<210> 347
<211> 198
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 347
Met Val Asp Arg Ala Thr Ser Thr Arg Ala Ala Phe Lys Ala Val Val
1 5 10 15
Glu Val Gly Pro Asp Ala Leu Asp Arg Ala Arg Ala Ala Glu Ala Val
20 25 30
Leu Gln Glu Ala Ile Ala Pro Ala Ala Gly Asp Phe Asp Phe Gly Ala
35 40 45
Val Arg Ser Ala Ala Arg Ala Leu Pro Asn Ser Ser Val Val Lys Ile
50 55 60
Ile Pro Gly Phe Gly Leu Gln Glu Thr Ala Pro Val Gln Val Met Val
65 70 75 80
Leu Thr Leu Lys Gln Ala Val Arg Gln Ala Leu Val Glu Pro Phe Glu
85 90 95
Asn Pro Ala Phe Trp Asp Arg Ala Asp Ala Ala Leu Ala Gln Val Phe
100 105 110
Leu Gly Leu Gly Glu Gln Glu Gly Ala Ala His Leu Ser Tyr Gln Asp
115 120 125
Ala Ala Asp Gly Thr Arg Tyr Phe Tyr Asn Leu Leu Phe Ala Leu Gln
130 135 140
Asp Glu Glu Thr Gly Asn His Leu Tyr Val Val Ala Phe Arg Val Glu
145 150 155 160
Val Ser Val Gly Ala Asp Lys Ala Glu Leu Leu Ser Leu Gly Ala Glu
165 170 175
Asp Thr Ala Ala Val Thr Ile Arg Leu Asp Ala Ile Ser Val Arg Gln
180 185 190
Glu Leu Ala Ala Thr Val
195
<210> 348
<211> 382
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 348
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ala Ser Gln Ala Ala Pro Ala Asn
20 25 30
Asn Ala Tyr His Ser Ile His Leu Ala Ala Asn Ala Asn Leu Lys Trp
35 40 45
Asp Val Tyr Gly Asp Gly Thr Tyr Asp Asp Ala Gly Ile Leu Leu Tyr
50 55 60
Asp Gly Ser Leu Gly Asp Asn Glu Gln Phe Leu Phe Phe Pro Leu Asp
65 70 75 80
Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Ala Val Thr
85 90 95
Thr Gly Ser Gly Trp Ile Ala Gly Asn Thr Met Ser His Asn Pro Glu
100 105 110
Ala Leu Lys Gln Ala Lys Trp Thr Gly Ala Asn Thr Asn Lys Trp Tyr
115 120 125
Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly Tyr
130 135 140
Gly Lys Val Ala Ser Tyr Gly Arg Ala Gly Ile Ala Gly Pro Arg Phe
145 150 155 160
Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Phe Asp Arg Leu Phe His
165 170 175
Ile Pro Ala Ala Ala Arg Ser Thr Phe Ser Leu Pro Thr Leu Pro Thr
180 185 190
Ile Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Ile Asp Gln
195 200 205
Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser Leu Leu
210 215 220
Pro Cys Ile Met Val Asn Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile
225 230 235 240
His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp Asp Arg
245 250 255
Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu Ser Tyr Ser Tyr
260 265 270
Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp Thr Leu
275 280 285
Ser Met Lys Phe Gly Ser Asp Leu Gly Ile Lys Phe Gly Asp Val Ser
290 295 300
Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile Ser
305 310 315 320
Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr Thr Ala Thr
325 330 335
Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu Ala Thr
340 345 350
Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Thr Val Ser Asp Pro Trp
355 360 365
Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys Thr Ser
370 375 380
<210> 349
<211> 355
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 349
Met Ala Pro Ala Asn Asn Ala Tyr His Ser Ile His Leu Ala Ala Asn
1 5 10 15
Ala Asn Leu Lys Trp Asp Val Tyr Gly Asp Gly Thr Tyr Asp Asp Ala
20 25 30
Gly Ile Leu Leu Tyr Asp Gly Ser Leu Gly Asp Asn Glu Gln Phe Leu
35 40 45
Phe Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser
50 55 60
Gly Lys Ala Val Thr Thr Gly Ser Gly Trp Ile Ala Gly Asn Thr Met
65 70 75 80
Ser His Asn Pro Glu Ala Leu Lys Gln Ala Lys Trp Thr Gly Ala Asn
85 90 95
Thr Asn Lys Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile
100 105 110
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Gly Arg Ala Gly Ile
115 120 125
Ala Gly Pro Arg Phe Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Phe
130 135 140
Asp Arg Leu Phe His Ile Pro Ala Ala Ala Arg Ser Thr Phe Ser Leu
145 150 155 160
Pro Thr Leu Pro Thr Ile Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr
165 170 175
Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val
180 185 190
Gly Ala Ser Leu Leu Pro Cys Ile Met Val Asn Asp Ser Gln Ala Ser
195 200 205
Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu
210 215 220
Glu Tyr Trp Asp Arg Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser
225 230 235 240
Glu Ser Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys
245 250 255
Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ser Asp Leu Gly Ile Lys
260 265 270
Phe Gly Asp Val Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile
275 280 285
Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg
290 295 300
Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln
305 310 315 320
Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Thr
325 330 335
Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg
340 345 350
Lys Thr Ser
355
<210> 350
<211> 371
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 350
Met Ser Asn Glu Asn Asn Met Asn Thr Ser Met Ile Asn Asp Glu Asn
1 5 10 15
Leu Ile Pro Ser Glu Val Leu Ala Ser Gly Lys Gln Ile Ser Phe Pro
20 25 30
Asp Phe Asn Phe Tyr Lys Ile Arg Thr Phe Cys Gly Lys Tyr Val Asp
35 40 45
Ile Arg Gly Gly Ser Thr Asp Asp Asp Ile Pro Ala Val Gln Tyr Thr
50 55 60
Gly Arg Asn Val Asp Asn Gln Lys Phe Leu Ile Phe Thr Leu Asp Asn
65 70 75 80
Asn Tyr Ser Val Ile Ala Ala Lys His Ser Gly Lys Val Leu Asp Ile
85 90 95
Ile Ser Leu Leu Phe Pro Phe Ala Leu Ile Gln Tyr Tyr Phe Lys Asn
100 105 110
Gly Asp Asn Gln Lys Phe Phe Ile Ala Asn Asp Gly Thr Ile Ala Val
115 120 125
Lys Ser Asp Gly Lys Val Leu Asp Ile Pro Asn Gly Ser Thr Lys Asp
130 135 140
Asn Val Ile Ile Ile Pro Tyr Gln Tyr His Gly Ser Ser Asn Gln Lys
145 150 155 160
Phe Thr Leu Glu Lys Tyr Gly Ser Ile Ser Ile Lys Pro Pro Thr Thr
165 170 175
Gly Thr Leu Pro Pro Ala Pro Asp Phe Lys Thr Asn Asp Ile Asn Glu
180 185 190
Glu Leu Pro Asp Asp Thr Pro Pro Val Ile Thr His Ala Thr Tyr Met
195 200 205
Pro Tyr Phe Met Val Lys Asp Pro Tyr Tyr Asn Ala Gln Gln Lys Ile
210 215 220
Gln Asn Thr Pro Tyr Tyr Ile Leu Val Arg Arg Gln Tyr Trp Gln Lys
225 230 235 240
Val Thr Gln Arg Ile Leu Ala Pro Ser Asp Thr Tyr Asp Tyr Ser Glu
245 250 255
Thr Thr Gly Val Ser Arg Thr Asp Gln Thr Ser Met Thr Glu Thr Thr
260 265 270
Glu Ile Ser Ile Gly Ala Asp Leu Gly Phe Met Phe Lys Gly Phe Ser
275 280 285
Val Gly Leu Ser Ala Ser Ile Thr Lys Ser Leu Ser Val Thr Lys Ser
290 295 300
Thr Ser Thr Thr Glu Ser Thr Glu Ile Thr Gln Lys Val Val Tyr Thr
305 310 315 320
Asn Pro Phe Lys His Gln Ile Ala Tyr Ala Lys Tyr Met Leu Ile Asn
325 330 335
Glu Tyr Tyr Val Thr Arg Ala Asn Gly Glu Met Ile Thr Ser Gly Asp
340 345 350
Ala Ser Tyr Trp Lys Val Pro Asp Pro Ser Arg Thr Val Ala Arg Ile
355 360 365
Ile Pro Arg
370
<210> 351
<211> 347
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 351
Met Pro Thr Ile Gln Ala Gly Ala Ala Tyr Asn Ile Ala Leu Lys Ala
1 5 10 15
Asn Gln Asn Leu Val Phe Asp Val Tyr His Asn Ile Met Asp Ser Gly
20 25 30
Gly Ile Ile Cys Leu Tyr Asn Lys Gly Ser Asn Tyr Leu Asn Gln Gln
35 40 45
Tyr Phe Phe Phe Ala Leu Asp Gly Gly Arg Tyr Ala Ile Val Pro Arg
50 55 60
Ser Ser Gly Lys Pro Val Glu Pro Ile Phe Ser Asp Ile Arg Arg Ile
65 70 75 80
Lys Pro Leu Gln Gln Arg Ser Trp Gly Gly Thr Asn Arg Gln Gln Trp
85 90 95
Tyr Leu Leu Asp Ala Pro Gly Ser Ser Val Glu Ile Phe Asn Gln Asp
100 105 110
Tyr Gly Gln Val Ala Ser Tyr Gly Lys Asp Gln Glu Leu Glu Tyr Pro
115 120 125
Asp Leu Asp Thr Ser Asn Pro Ser Asp Pro Asn Arg Thr Trp Tyr Leu
130 135 140
Gln Pro Val Tyr Gln Ala Thr Leu Pro Gln Leu Val Ala Thr Lys Pro
145 150 155 160
Ile Gly Ser Pro Pro Gln Tyr Ala Gly Asp Pro Tyr Gln Val Leu Pro
165 170 175
Asp Gln Ser Glu Val Thr Asn Val Ser Asn Ala Leu Ile Pro Cys Ile
180 185 190
Leu Val Asn Asp Gly Ser Leu Ser Asp Ala Thr Lys Val Gln Gln Ser
195 200 205
Pro Tyr Tyr Leu Met Ile Lys Gln Gln Phe Trp Gln Lys Ala Ala Phe
210 215 220
Gly Thr Val Ala Pro Asn Ser Lys Arg Ser Phe Ser Phe Lys Thr Gly
225 230 235 240
Ser Thr Ser Ile Asp Gln Lys Ser Met Ser Asp Thr Leu Ser Ile Thr
245 250 255
Val Gly Gly Asp Leu Gly Leu Gln Phe Lys Gly Ala Ser Ala Ser Ala
260 265 270
Lys Phe Glu Ile Ser Arg Gln Ile Gln Thr Val Val Ser Ser Thr Asn
275 280 285
Glu Gln Met Ser Glu Tyr Thr Asp Thr Ile Glu Phe Asp Asn Ser Ser
290 295 300
Gly Thr Thr Leu Lys Gly Ile Ser Ile Tyr Gln Leu Val Thr Arg Tyr
305 310 315 320
Ala Leu Tyr Arg Thr Asn Gly Ser Ala Val Ser Asp Pro Trp Lys Phe
325 330 335
Ile Asp Asn Lys Ser Ala Val Met Val Val Ser
340 345
<210> 352
<211> 368
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 352
Met Pro Asp Asn Asn Arg Tyr Tyr Ser Val His Leu Ala Ala Asp Thr
1 5 10 15
Ala Leu Lys Trp Asp Val Tyr Gly Asn Gly Thr Tyr Asp Asp Ala Gly
20 25 30
Ile Leu Leu Tyr Asn Gly Ser Leu Gly Asp Asn Glu Gln Phe Val Phe
35 40 45
Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Ala Val Thr Ile Gly Gly Gly Trp Val Gly Asp Asn Ala Phe Ser
65 70 75 80
Tyr Asn Arg Glu Gly Leu Thr Gln Ser Lys Trp Thr Gly Ala Ala Ala
85 90 95
Asn Lys Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile Val
100 105 110
Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile Thr
115 120 125
Gly Pro Lys Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn Asp
130 135 140
Arg Leu Phe Lys Ile Gln Lys Ser Ala Leu Pro Leu Pro Asp Gly Val
145 150 155 160
Gln Leu Pro Gly Trp Pro Glu Tyr Gly Thr Phe Ser Leu Pro Gln Leu
165 170 175
Pro Ala Thr Gly Asn Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly Ile
180 185 190
Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala Ser
195 200 205
Leu Leu Pro Cys Ile Met Val Asn Asp Ser Gln Ala Ser Asp Tyr Thr
210 215 220
Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr Trp
225 230 235 240
Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu Ser Tyr
245 250 255
Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met Thr Asp
260 265 270
Thr Leu Ser Met Lys Phe Gly Ala Asp Leu Gly Ile Lys Phe Gly Asp
275 280 285
Val Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln Thr Glu
290 295 300
Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser Tyr Thr
305 310 315 320
Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu
325 330 335
Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Thr Val Ser Asp
340 345 350
Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys Thr Ser
355 360 365
<210> 353
<211> 288
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 353
Met Ala Glu Asn Thr Pro Ile Lys Val Asn Glu Asn Val Lys Val Gly
1 5 10 15
Ile Thr Asp Val Gln Ser Glu Leu Lys Lys Ile Gly Thr Tyr Tyr Tyr
20 25 30
Thr Asn Glu Ile Ala Gly Thr Lys Ile Glu Ser Trp Pro Thr Leu Thr
35 40 45
Phe Lys Ala Ser Pro Asp Ser Val Glu Thr Lys Val Asn Phe Ser Ile
50 55 60
Thr Gly Thr Thr Asn Lys Leu Asn Phe Asp Ser Asn Ile Val Glu Tyr
65 70 75 80
Val Gly Glu Asn Glu Phe Glu Asn Ile Thr Asp Gln Thr Gln Lys Phe
85 90 95
Ser Thr Ala Lys Tyr Thr Lys Ser Val Thr Glu Ser Ile Ser Thr Ala
100 105 110
Thr Thr Lys Gly Phe Asn Val Gly Gly Ser Ser Asn Ile Phe Thr Ile
115 120 125
Pro Leu Ile Leu Asn Asn Gly Ile Thr Val Asn Ala Gly Phe Asn Ser
130 135 140
Ser Thr Thr Glu Thr Gln Thr Lys Ala Glu Gln Lys Thr Ile Glu Ala
145 150 155 160
Ser Pro Gln Ser Ile Glu Val Pro Pro His Lys Lys Tyr Lys Ala Asp
165 170 175
Val Val Leu Glu Gln Arg Asn Phe Trp Gly Asp Ile Thr Phe Asn Gly
180 185 190
Val Gly Ser Asn Pro Met Thr Thr Ile Lys Gly Thr Ala Ser Tyr Trp
195 200 205
Ala Pro Asn Gly Met Gly Ala Trp Lys Gln Tyr Thr Phe Ser Asp Lys
210 215 220
Thr Lys Asn Tyr Trp Asn Lys Leu Thr Thr Ser Gln Lys Asn Ser Leu
225 230 235 240
Asn Gly Ile Lys Phe Glu Asn Asn Asp Val Lys Ala Glu Gly Thr Ala
245 250 255
Lys Ile Glu Gly Ile Phe Gly Ser Asn Leu Ile Val Arg Val Tyr Asp
260 265 270
Ile Thr Asp Lys Ser Asn Pro Lys Leu Val Glu Thr Lys Ser Phe Asn
275 280 285
<210> 354
<211> 390
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 354
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Gly Val Gly Ala
1 5 10 15
Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Ala Pro Ala
20 25 30
Asn Asn Glu Tyr His Thr Ile His Leu Ala Ala Asn Ala Asn Ile Ala
35 40 45
Trp Asp Val Tyr Lys Gly Trp Gln Ser Asp Asp Ala Gly Ile Val Leu
50 55 60
Trp Asp Gly Ala Arg Gly Asp Asn Glu Gln Phe Val Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Ile Gly Ser Ala Ile Ile Gly Ser Asp Gly Val Arg Asn Ile Pro
100 105 110
Ser Phe Asn Asn Glu Gly Leu Arg Gln Thr Ser Trp Thr Gly Ala Pro
115 120 125
Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Phe Glu Ile
130 135 140
Val Asn Gln Gly Ser Gly Lys Val Ala Ser Trp Ala Gly Val Glu Met
145 150 155 160
Phe Gly Tyr Lys Asp Tyr Val Asp Leu Asp Glu Ser Asn Pro Ser Asp
165 170 175
Asn Asp Arg Leu Phe Asn Ile Pro Ala Ala Arg Ser Thr Phe Ser Leu
180 185 190
Pro Thr Leu Pro Thr Val Gly Thr Arg Pro Asn Ala Pro Glu Tyr Asn
195 200 205
Thr Ser Gly Ala Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val
210 215 220
Val Val Gly Ala Ser Leu Ile Pro Ser Ile Met Val Lys Asp Asn Gly
225 230 235 240
Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Val Leu Val
245 250 255
Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu Ile Ile Pro Ala Gly
260 265 270
Ala Thr Ser Lys Tyr Thr Val Lys Ser Gly Val Ser Glu Ile Asp Gln
275 280 285
Lys Lys Met Ser Asp Thr Leu Ala Met Ser Phe Gly Ser Asp Leu Gly
290 295 300
Leu Lys Phe Gly Glu Ser Ser Leu Ser Ile Lys Ser Ser Val Ser Arg
305 310 315 320
Thr Ile Gln Thr Glu Val Thr Lys Thr Ser Thr Asn Ala Thr Glu Glu
325 330 335
Thr Glu Glu Lys Ser Ile Pro Ser Ile Pro Gly Gln Asn Thr Gly Phe
340 345 350
Thr Ala Tyr Gln Leu Val Thr Lys Tyr Thr Leu Lys Arg Thr Asp Gly
355 360 365
Ser Val Val Thr Asp Pro Ser Ile Val Arg Asp Ser Lys Gln Thr Ile
370 375 380
Ala Arg Thr Ile Ala Lys
385 390
<210> 355
<211> 378
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 355
Met Pro Glu Asn Asn Arg Tyr Tyr Ser Leu His Leu Thr Asn Asn Thr
1 5 10 15
Glu Lys Val Trp Asp Val Tyr Gly Gly Trp Ala Ser Asn Asp Ala Gly
20 25 30
Ile Leu Leu Tyr Asn Ala Asn Gly Lys Asp Asn Gln Gln Phe Val Phe
35 40 45
Phe Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Pro Val Gly Leu Gly Gly Gly Ile Asn Tyr Ser Asp Gly Val Arg
65 70 75 80
Asn Met Ser Asn Tyr Asn Gly Asn Pro Ala Asp Ile Leu Gly Gln Asn
85 90 95
Asp Trp Thr Gly Ala Asp Thr Asp Lys Trp Tyr Leu Arg Asp Lys Gly
100 105 110
Asn Asn Asn Tyr Glu Ile Val Asn Gln Gly Lys Gly Lys Val Ala Ser
115 120 125
Tyr Ala Ala Thr Asn Ile Ala Pro Tyr Leu His Tyr Val Asp Leu Asp
130 135 140
Glu Ser Asn Pro Ser Asp Gly Asp Arg Leu Phe Arg Ile Gln Lys Asn
145 150 155 160
Val Leu Pro Leu Pro Asp Gly Leu Gln Leu Pro Gly Phe Ser Asp Tyr
165 170 175
Gly Thr Phe Ser Leu Pro Lys Leu Pro Ala Thr Gly Asn Arg Pro Asp
180 185 190
Ala Pro Asn Tyr Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser
195 200 205
Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro Cys Phe Met Val Lys
210 215 220
Asp Ser Gln Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr
225 230 235 240
Thr Leu Ile Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile
245 250 255
Pro Ala Gly Leu Thr Arg Asn Tyr Thr Phe Lys Thr Gly Met Thr Ser
260 265 270
Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala
275 280 285
Asp Phe Gly Leu Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Thr Glu
290 295 300
Ile Ser Arg Thr Ile Gln Thr Glu Ile Thr Ser Thr Asn Thr Glu Ala
305 310 315 320
Ser Glu Glu Thr Ile Ser Ser Thr Val Thr Ser Glu Pro Gly Lys Ser
325 330 335
Gln Thr Gly Phe Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys
340 345 350
Arg Ala Asp Gly Ser Ile Val Ser Asp Pro Trp Val Val Lys Asn Asn
355 360 365
Lys Ile Thr Ile Ala Arg Lys Lys Ser Glu
370 375
<210> 356
<211> 341
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 356
Met Thr Asn Gly Leu Asp Ser Asn Thr Gln Asp Ser Asn Glu Leu Asp
1 5 10 15
Asn Gly Val Leu Ala Leu Asp Pro Phe Thr Phe Tyr His Ile Arg Thr
20 25 30
Phe Cys Gly Lys Thr Leu Asp Val Thr Asn Ser Ser Ile Ala Asp His
35 40 45
Ala Met Ile Ile Gln His His Ser Asn Asn Gln Leu Asn Gln Gln Phe
50 55 60
Leu Ile Phe Thr Leu Asp Asp Gly Tyr Ser Ile Ile Thr Ser Val Ile
65 70 75 80
Ser Gly Lys Val Leu Asp Ile Ser Gln Ser Gly Ser Leu Lys Gly His
85 90 95
Val Ile Gln His Tyr Leu Asn His Gly Asn His Gln Lys Phe Leu Val
100 105 110
Gly Asn Asn Gly Thr Ile Ala Ala Lys His Ser Gly Lys Val Phe Asp
115 120 125
Val Tyr Lys Ala Ser Thr Ala Asn Asn Val Pro Ile Lys Glu Asp Asn
130 135 140
Pro His Asn Gly Ala Asn Gln Lys Phe Thr Phe Glu Arg Val Arg Thr
145 150 155 160
Phe Thr Val Pro Ser Pro Ser Lys Gly Thr Leu Pro Pro Ala Pro Asp
165 170 175
Phe Lys Asn Asp Ile Asn Glu Val Leu Pro Asp Lys Thr Asn Pro Val
180 185 190
Ile Thr His Asp Thr Asn Ile Pro Cys Ile Met Ile Asp Asp Lys Thr
195 200 205
Phe Ser Ser Pro Gln Lys Ile Gln Tyr Ser Pro Tyr Tyr Lys Leu Val
210 215 220
Lys Ile Gln Tyr Trp Glu Lys Val Thr Gln Arg Ile Leu Gly Pro Arg
225 230 235 240
Asp Thr Tyr Ser Tyr Gln Lys Ser Lys Gly Val Ser Arg Thr Asp Gln
245 250 255
Ser Ser Met Thr Glu Thr Val Ser Met Ser Ile Gly Ala Asp Phe Gly
260 265 270
Phe Met Phe Lys Gly Phe Ser Ala Ser Leu Ser Thr Arg Val Thr Lys
275 280 285
Glu Leu Ser Val Thr Lys Ser Thr Ser Tyr Thr Glu Met Thr Glu Glu
290 295 300
Thr Tyr Thr Glu Ser Tyr Thr Asn Pro Phe Asn Tyr Ala Leu Ala Arg
305 310 315 320
Ala Gln Tyr Met Leu Val Asn Glu Phe Tyr Val Thr Arg Met Gly Trp
325 330 335
Tyr Thr Tyr Asn Lys
340
<210> 357
<211> 778
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 357
Met Ser Ser Asn Thr Met Val Pro Tyr Asn Val Leu Arg Asn Leu Asp
1 5 10 15
Met Pro Thr Ile Ser Gly Thr Arg Trp Asn Lys Glu Met Phe Thr Asn
20 25 30
Ala Leu Asp Asn Thr Ser Ser Leu Leu Thr Leu Ile Glu Lys Gly Ile
35 40 45
Asn Asp Gly Asp Asp Val Leu Gly Leu Leu Ser Phe Ile Gly Leu Thr
50 55 60
Ala Leu Glu Ala Ile Pro Val Val Gly Gly Val Leu Ser Ser Ile Val
65 70 75 80
Ser Met Leu Phe Phe Pro Thr Lys Ser Ser Ile Asp Phe Lys Lys Ile
85 90 95
Trp Glu His Leu Glu Lys Ala Ile Asn Gln Ile Val Asp Lys Lys Ile
100 105 110
Glu Glu Ala Leu Met Ser Gln Leu Met Gln Glu Ile Ala Gly Leu Ala
115 120 125
Asn Val Leu Glu Glu Tyr Arg Asp Ala Tyr Asp Ser Tyr Asn Gly Lys
130 135 140
Lys Val Phe Asp Ile Gln Asp Arg Leu Thr Pro Glu Glu Tyr Leu Val
145 150 155 160
Gln Val Phe Thr Ser Ala Asn Leu Leu Phe Ile Gln Arg Ile Pro Thr
165 170 175
Phe Gln Asn Pro Lys Tyr Asp Val Leu Phe Leu Pro Phe Phe Val His
180 185 190
Val Ala Glu Met His Ile Leu Leu Ile Arg Asp Ala Ala Ile His Gly
195 200 205
Leu Glu Trp Gly Met Asp Glu Glu Ile His Gln Lys Phe Lys Arg Asp
210 215 220
Phe Lys Lys Ile Ile Asp Lys Tyr Thr Asn His Leu Leu Asn Thr Tyr
225 230 235 240
Lys Lys Gly Leu Lys Glu Val Ser Glu Arg Glu Leu Asn Asp Lys Asp
245 250 255
Phe Pro Thr Thr Asn Asn Lys Tyr His Tyr Ile Asn Thr Val Arg Trp
260 265 270
Asn Val Ile Asn Gln Tyr Lys Arg Gly Met Thr Leu Thr Val Phe Asp
275 280 285
Phe Ala Tyr Lys Trp Lys Tyr Tyr Gln Glu Ile Tyr Gln Asn Asn Ile
290 295 300
Glu Leu Asn Pro Phe Arg Thr Ile Tyr Ser Asp Ile Ala Gly Ser Val
305 310 315 320
Tyr Pro Tyr Glu Lys Ala Thr Asn Glu Ile Asp Thr Ile Ile Lys Asp
325 330 335
Asn Asn Leu Lys Tyr Arg Gly Ile Phe Lys Arg Leu Gln Val Asn Tyr
340 345 350
Ala Glu Arg Ile Asp Ser Ile Gln Ser Ser Tyr Ile Gln Asn Asn Glu
355 360 365
Ile Ile Asp Ser Glu Arg Thr Gly Gly Thr Gly Gly Arg Glu Ser Val
370 375 380
Leu Asp Ile Glu Ser Pro Val Lys Asn Pro Leu Met Gln Val Asn Met
385 390 395 400
Trp Ser Glu Leu Val Pro Ile Ser Leu Gly Phe Lys Phe Tyr Asn Glu
405 410 415
Lys Glu Lys Asn Ile Gly Gly Gly Ser Trp Trp Pro Asn Pro His Lys
420 425 430
Phe Gly Ala Tyr Gln Tyr Val Gly Asn Lys Val Ser Ser Ile Met Gly
435 440 445
Phe Gly Lys Asn Ala Thr Gly Gly Phe Asn Ser Leu Asp Ala Met Val
450 455 460
Val Gly Phe Lys Arg Asp Asp Tyr Thr Pro Glu Asn Arg Phe Val Gly
465 470 475 480
Val Ser Lys Asn Asp Glu Pro Ile Thr Lys Val Ile Asp Val Gly Asn
485 490 495
Tyr His Gln Gly Lys Phe Glu Ser Asn Ile Thr Met Ile Asp Glu Pro
500 505 510
Met Phe Gly Asp Gly Val Leu Gln Leu Glu Asn Tyr Ser Asn Asn Leu
515 520 525
Asn Gln Asp Ser Tyr Val Thr Tyr Gln Ile His Thr Glu Thr Ala Gly
530 535 540
Thr Tyr Glu Leu His Ala Val Ile Gly Ala Glu Lys Gln Lys Asp Lys
545 550 555 560
Val Val Leu Lys Val Ser Ile Asn Gly Asn His Ser Glu Asn Ile Thr
565 570 575
Thr Asp Phe Phe Asp Asn Gly Asp Ile Trp Asp Gly Met Ser Leu Asn
580 585 590
Asp Gly Phe Val Tyr Lys Arg Val Leu Ile Gly Arg Phe Gln Phe Lys
595 600 605
Lys Gly Leu Asn Asn Ile Thr Ile Tyr Asn Gly Val Leu Gln Thr Ser
610 615 620
Ala Ser Ile Lys Ile Trp Asn Leu Ala Ala Leu Glu Leu Thr Ile Pro
625 630 635 640
Asp Asn Thr Glu Trp Lys Phe Gln Asn Gly Asp Phe Ser Lys Gly Leu
645 650 655
Lys Gly Trp Thr Val Glu Glu Gly Ser Pro Ser Ala Gly Asn Gln Tyr
660 665 670
Leu Asp Met His Gln Asn Ser Lys Val Ser Gln Thr Ile Lys Val Asn
675 680 685
Pro Asn Thr Thr Tyr Glu Leu Ser Tyr Asp Ser Ile Thr Pro Ser Ser
690 695 700
Asn Asn Phe Phe Gly Tyr Gly Thr Leu Tyr Leu Asp Phe Leu Ser Met
705 710 715 720
Asp Gly Ser Lys Gln Ser Gly Val Ala His Pro Ile Glu Gln Gln Gly
725 730 735
Asn Ser Trp Ala Arg Lys Thr Tyr Lys Phe Thr Thr Pro Ser Asp Ile
740 745 750
Lys Lys Leu Lys Leu Thr Ile Ala Asn Glu Ala Ser Asn Gln Gly Phe
755 760 765
Asn Ile Asp Asn Ile Ala Val Thr Glu Gly
770 775
<210> 358
<211> 301
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 358
Met Asn Gln Asp Glu Lys Ile Gln Arg Glu Tyr Phe Pro Glu Asn Asp
1 5 10 15
Pro Leu Val Gly Gly Ile Ala Gly Thr Ile Gly Thr Met Gly Gly Val
20 25 30
Ile Gly Gly Ala Ala Leu Ala Ser Arg Val Asp Tyr Gly Leu Lys Ser
35 40 45
Gln Leu Asn Gln Val Asn Ile Thr Ser Val Gly Thr Asp Phe Lys Val
50 55 60
Thr Pro Ser Lys Val Val Glu Glu Asp Gln Gly Val Ile Asn Leu Leu
65 70 75 80
Ser Tyr Arg Asn Asn Thr Pro Glu Lys Gln Lys Ala Phe Thr Thr Glu
85 90 95
Glu Ser Tyr Lys Lys Val Asp Thr Val Thr Ala Thr Asn Gln Tyr Gly
100 105 110
Phe Lys Leu Ser Ala Lys Ser Val Thr Lys Phe Gly Phe Asp Ile Gly
115 120 125
Leu Ala Lys Gly Glu Gln Thr Phe Glu Val Ser Ala Glu Phe Ala Phe
130 135 140
Asn Arg Thr Asp Thr Phe Thr Thr Thr Asn Glu Lys Asn Val Thr Phe
145 150 155 160
Lys Ala Gln Glu Val Ile Ala Ala Pro Gly Gly Thr Thr Asp Tyr Phe
165 170 175
Ser Arg Val Lys Lys Ala Lys Phe Ser Gly Ser Phe Gln Thr Asp Ala
180 185 190
Ala Leu Asp Ser Leu Lys Val Lys Leu Pro Ile Val Lys Gly Gly Asn
195 200 205
Met Asn Val Thr His Thr Glu Glu Val Thr Leu Asn Ser Glu Asp Leu
210 215 220
Tyr Asn Ile Phe Lys Leu Thr Ser Lys Asp Phe Pro Leu Pro Ser Tyr
225 230 235 240
Leu Ser Phe Asp Asp Thr Asn Lys Ser Ile Leu Val Lys Asn Ala Thr
245 250 255
Phe Asn Tyr Thr Gly Glu Ile Gly Tyr His Thr Ala Ile Asp Ala Lys
260 265 270
Phe Thr Pro Phe Asp Gln Thr Lys Ala Thr Ala Val Met Pro Tyr Ala
275 280 285
Lys Tyr Ala Glu Lys Thr Gln Ala Asn Ala Leu Leu Arg
290 295 300
<210> 359
<211> 362
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 359
Met Glu Thr Pro Glu Lys Asn Lys Tyr Tyr Ser Ile Tyr Ser Lys Val
1 5 10 15
Asn Ser Thr Leu Ala Trp Thr Pro Tyr Ser Ser Ser Tyr Asp Gly Ala
20 25 30
Ile Thr Asn Val Ile Lys Leu Asn Met Glu Asp Asn Gln Lys Tyr Leu
35 40 45
Phe Phe Pro Leu Asn Gly Gly Ser Tyr Ala Ile Val Asn Lys Ser Ser
50 55 60
Gly Lys Val Ala Ser Ile His Thr Asn Leu Val Val Pro Arg Val Gly
65 70 75 80
Ser Phe Ser Cys Asn Gly Ser Ser Gly Gln Lys Trp Asn Leu Arg Asp
85 90 95
Lys Gly Asn Gly Tyr Tyr Glu Phe Met Asn Gln Gly Asn Gly Lys Val
100 105 110
Ala Ala Thr Thr Leu Asn Ser Ser Gly Ala Ile Gln Asp Leu Ser Val
115 120 125
Gly Phe Gly Asn Ser Asn Pro Ser Asp Pro Glu Lys Leu Phe Ser Phe
130 135 140
Ser Ser Pro Ile Ala Ser Ile Met Tyr Gln Thr Leu Asn Met Glu Glu
145 150 155 160
Asn Pro Thr Phe Ser Leu Pro Asn Leu Pro Asn Thr Gly Thr Arg Pro
165 170 175
Ala Ala Pro Glu Tyr Thr Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr
180 185 190
Ser Asn Ser Val Val Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val
195 200 205
Asn Asp Asn Ser Val Asp Glu Tyr Thr Lys Leu His Asn Ser Pro Tyr
210 215 220
Tyr Thr Leu Ile Lys Glu Glu Phe Trp Asp Lys Thr Phe Ser Ala Val
225 230 235 240
Ile Pro Ser Gly Leu Thr Arg Asn Tyr Ala Phe Lys Thr Gly Met Thr
245 250 255
Thr Val Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly
260 265 270
Ala Asp Leu Gly Leu Lys Phe Gly Asp Leu Thr Ala Ala Val Lys Ala
275 280 285
Glu Ile Ser Arg Thr Ile Gln Thr Glu Ile Ser Thr Thr Asn Thr Glu
290 295 300
Ala Ser Glu Glu Thr Ile Thr Ser Thr Val Ala Ser Glu Pro Gly Lys
305 310 315 320
Thr Thr Gly Phe Thr Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys
325 330 335
Arg Ala Asp Gly Ser Leu Val Ser Glu Pro Trp Ile Val Lys Asn Asn
340 345 350
Lys Ile Thr Ile Ala Arg Lys Asn Ala Gln
355 360
<210> 360
<211> 270
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 360
Met Asp Thr Ser Arg Leu Thr Val Glu Asn Ala Glu Ser Tyr Gly Phe
1 5 10 15
Glu Ile Lys Glu Ser Lys Pro Gln Gly Thr Met Phe Met Gly Glu Ser
20 25 30
Thr Leu Asn Asn Arg Thr Asp Glu Thr Gln Thr Leu Asn Ser Asp Ser
35 40 45
Phe Thr Lys Thr Leu Thr Asp Ser Ile Thr Leu Ser Val Thr Asn Gly
50 55 60
Ile Thr Thr Thr Val Asn Ile Ser Ile Ser Gly Lys Ile Phe Gly Met
65 70 75 80
Gly Ala Glu Thr Ser Met Ser Phe Glu Val Ser Thr Ser Thr Thr Asn
85 90 95
Glu Gln Thr Asn Glu Glu Ser Val Ala Tyr Thr Val Pro Ser Gln Thr
100 105 110
Ile Val Val Pro Ala Gln Thr Thr Tyr Tyr Val His Thr Leu Leu Glu
115 120 125
Arg Asn Gln Leu Glu Gly Ile Cys Arg Leu Arg Ala Asp Leu Thr Gly
130 135 140
Phe Phe Phe Val Leu Thr Lys Phe Ala Gly Lys Glu Val Pro Cys Tyr
145 150 155 160
Phe Gly Glu Ile Tyr Asp Phe Val Asn Gln Tyr Gln Ser Leu Leu Pro
165 170 175
Leu Pro Ser Gly Ile Ser Leu Asn His Ser Lys Lys Ser Ile His Phe
180 185 190
Glu Gly Ile Ala Glu Tyr Phe Tyr Gly Thr Gly Thr Lys Phe His Ile
195 200 205
Thr Val Ser Asp Lys Pro Ser Ser Gln Gly Thr Gln Glu His Lys Ser
210 215 220
Ile Asp Thr Lys Thr Gly Leu Gly Thr Tyr Pro Ile Gly Leu Asn Gly
225 230 235 240
Lys Asn Leu Asn Leu Asp Leu Asn Asp Leu Lys Glu His Met Asp Pro
245 250 255
Lys Asp Phe Glu Lys Leu Lys Glu Leu Gln Asn Glu Lys Val
260 265 270
<210> 361
<211> 296
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 361
Met Ala Ile Val Asn Leu Asp Glu Tyr Leu Phe Ser Leu Ala Lys Lys
1 5 10 15
Ala Ile Thr Ser Gly Val Ile Pro Thr Ser Val Asp Thr Ser Arg Leu
20 25 30
Thr Val Glu Asn Ala Glu Ser Tyr Gly Phe Glu Ile Lys Glu Ser Lys
35 40 45
Pro Gln Gly Thr Met Phe Met Gly Glu Ser Thr Leu Asn Asn Arg Thr
50 55 60
Asp Glu Thr Gln Thr Leu Asn Ser Asp Ser Phe Thr Lys Thr Leu Thr
65 70 75 80
Asp Ser Ile Thr Leu Ser Val Thr Asn Gly Ile Thr Thr Thr Val Asn
85 90 95
Ile Ser Ile Ser Gly Lys Ile Phe Gly Met Gly Ala Glu Thr Ser Met
100 105 110
Ser Phe Glu Val Ser Thr Ser Thr Thr Asn Glu Gln Thr Asn Glu Glu
115 120 125
Ser Val Ala Tyr Thr Val Pro Ser Gln Thr Ile Val Val Pro Ala Gln
130 135 140
Thr Thr Tyr Tyr Val His Thr Leu Leu Glu Arg Asn Gln Leu Glu Gly
145 150 155 160
Ile Cys Arg Leu Arg Ala Asp Leu Thr Gly Phe Phe Phe Val Leu Thr
165 170 175
Lys Phe Ala Gly Lys Glu Val Pro Cys Tyr Phe Gly Glu Ile Tyr Asp
180 185 190
Phe Val Asn Gln Tyr Gln Ser Leu Leu Pro Leu Pro Ser Gly Ile Ser
195 200 205
Leu Asn His Ser Lys Lys Ser Ile His Phe Glu Gly Ile Ala Glu Tyr
210 215 220
Phe Tyr Gly Thr Gly Thr Lys Phe His Ile Thr Val Ser Asp Lys Pro
225 230 235 240
Ser Ser Gln Gly Thr Gln Glu His Lys Ser Ile Asp Thr Lys Thr Gly
245 250 255
Leu Gly Thr Tyr Pro Ile Gly Leu Asn Gly Lys Asn Leu Asn Leu Asp
260 265 270
Leu Asn Asp Leu Lys Glu His Met Asp Pro Lys Asp Phe Glu Lys Leu
275 280 285
Lys Glu Leu Gln Asn Glu Lys Val
290 295
<210> 362
<211> 369
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 362
Met Asn Phe Lys Val Gly Thr Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Ser Gly Asn Ser Thr Ala Asn Asn Gly Asn
20 25 30
Val Gln Gln Tyr Glu Phe Ile Pro Asp Gly Pro Ser Glu Arg Phe Phe
35 40 45
Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Ile Ser Gly Asn Ser Thr Ala Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ser Pro Ser Glu Tyr Trp Tyr
85 90 95
Phe His Arg Glu Ala Asp Gly Tyr Tyr Val Ile Glu Ser Lys Leu Ser
100 105 110
Gly Lys Val Leu Asp Ile Lys Gly Asn Ser Thr Ala Asn Asn Ala Asn
115 120 125
Val Gln Gln Tyr Gln Phe Ile Pro Asp Ser Pro Ala Glu Arg Phe Ala
130 135 140
Ala Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile Asn Thr Lys Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp Gln Leu Pro Ala
165 170 175
Glu Thr Glu Arg Val Val Thr Ala Phe Ser Ile Val Pro Ala Ile Ser
180 185 190
Val Lys Asp Pro His Tyr Gly Asn Asp Thr Val Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Glu
210 215 220
Ser Tyr Val Leu Ala Pro Ser Glu Lys Tyr Ile Phe Glu Thr Lys Thr
225 230 235 240
Gly Ile Lys Gln Ile Asp Gln Glu Thr Ala Thr Lys Thr Val Ser Trp
245 250 255
Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly Phe Ser Leu Gly
260 265 270
Leu Ser Thr Gln Tyr Ser Thr Gln Leu Gln Asn Ser Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Val Asn Arg His Glu Ile Ile Asn Pro
290 295 300
Phe Ser Glu Arg Met Ala Tyr Ser Arg Tyr Ile Leu Thr Thr Glu Tyr
305 310 315 320
Ser Val Gln Arg Lys Asn Gly Glu Glu Leu Phe Gly Tyr Tyr Arg Gln
325 330 335
Lys His Pro Lys Leu Phe Asp Lys Gln Lys Leu Tyr Thr Tyr Glu Glu
340 345 350
Leu Lys His Arg Ala Val Asn Tyr Cys Ser Ser His Leu Val Ile His
355 360 365
Leu
<210> 363
<211> 291
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 363
Met Ala Gln Ile Ser Pro Val Gln Asn Thr Ala Gln Gln Gln Ser Val
1 5 10 15
Ser Ile Ser Asp Trp Arg Glu Pro Phe Gln Lys Thr Tyr Lys Ala Ala
20 25 30
Lys Leu Arg Asp Pro Asn Arg Phe Phe Ser Asn Glu Ala Thr Leu Asp
35 40 45
Leu Ser Ser Ile Tyr Gln Ser Ser Val Glu Ala Asp Gly Ser Pro Thr
50 55 60
Ile Thr Asn Ser Lys Ser Leu Phe Val Gly Arg Thr Thr Leu Thr Asn
65 70 75 80
Arg Thr Asn Arg Asp Gln Thr Leu Thr Thr Asp Ser Phe Ser Lys Thr
85 90 95
Phe Gly Asn Ser Val Ser Asn Ser Thr Thr Asn Gly Phe Asn Leu Gly
100 105 110
Val Ser Thr Ser Ala Ser Phe Gly Ile Pro Leu Ile Gly Glu Thr Ser
115 120 125
Ile Glu Ile Ser Thr Glu Tyr Asn Phe Ser Asn Thr Ala Glu Lys Thr
130 135 140
Lys Thr Glu Ser Tyr Thr Tyr Thr Ala Ser Pro Gln Asn Ile Val Val
145 150 155 160
Pro Ala Asn Ser Ser Val Glu Val Ile Val Asn Leu Asn Thr Ala Lys
165 170 175
Ile Ser Gly Asn Val Lys Leu Leu Ser His Val Asp Gly Gln Val Ser
180 185 190
Tyr Arg Asn Asn Lys Tyr Asn Lys Ile Ser Thr Ser Phe Gly Gly Phe
195 200 205
Leu Lys His Ser Val Arg Pro Asn Glu Ile Ala Ser Asn Val Lys Val
210 215 220
His Pro Thr Ser Lys Gly Ala Tyr Leu Ile Gly Lys Gly Lys Tyr Ser
225 230 235 240
Ala Glu Tyr Gly Thr Glu Phe Ser Val Thr Val Arg Pro Val Ala Lys
245 250 255
Pro Asn Thr Leu Ala Ala Lys Ser Ser Ser Met Val Ser Glu Asn Ser
260 265 270
Lys Ala Thr Ser Glu Gly Tyr Thr Tyr Thr Val Lys Pro Glu Val Lys
275 280 285
Lys Glu Gln
290
<210> 364
<211> 402
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 364
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ile Ser Glu Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Asn Ile Ala Leu Lys Ser Asn Pro Lys Lys Val
35 40 45
Trp Asn Val Ser Gln Ala Ser Asn Asp Asn Asp Arg Ala Ile Val Leu
50 55 60
Trp Gln Gly Gly Ser Ala Asp His Glu Arg Phe Ala Phe Phe Gln Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Val Val
85 90 95
Thr Phe Gly Asn Gly Ser Trp Val Ser Gly Gly Pro Ile Pro Ala Asn
100 105 110
Glu Thr Leu Gln Gln Gln Lys Trp Thr Gly Glu Ala Lys Gln Lys Trp
115 120 125
Asn Leu Arg Asp Leu Gly Ser Asn Gly Gln Gly Ser Asn Asn Tyr Glu
130 135 140
Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg Trp Ile Gly
145 150 155 160
Ser Val Val Asp Asn Thr Glu Tyr Leu Gln Leu Asp Asn Ser Asn Pro
165 170 175
Ser Asp Pro Asp Lys Val Phe Thr Leu Phe Asn Gly Thr Leu Asn Ile
180 185 190
Phe Gly Gln Asn Met Thr Phe Phe Asn Asp Thr Ile Ser Val Pro Lys
195 200 205
Leu Pro Ala Thr Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr Gly Gly
210 215 220
Val Asp Gln Gln Leu Pro Gln Val Ser Asp Ser Val Val Val Gly Ala
225 230 235 240
Ser Leu Ile Pro Cys Ile Met Val Asn Asp Asn Gln Ala Ser Asp Tyr
245 250 255
Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr
260 265 270
Trp Asp Lys Thr Tyr Ser Ala Val Ile Pro Ala Gly Val Thr His Asn
275 280 285
Tyr Ser Phe Thr Thr Gly Met Ser Ser Val Asp Gln Gln Lys Met Thr
290 295 300
Asp Thr Leu Ser Met Ser Ile Gly Ala Asp Phe Gly Leu Ser Phe Gly
305 310 315 320
Gly Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr Ile Gln Thr
325 330 335
Glu Ile Thr Ser Thr Asn Thr Asn Ala Ser Glu Glu Lys Val Ala Arg
340 345 350
Ala Val Glu Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr Glu Tyr Gln
355 360 365
Leu Ala Thr Arg Tyr Thr Leu Lys Arg Ala Asp Gly Ser Asn Val Ser
370 375 380
Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile Thr Arg Lys Asn
385 390 395 400
Ala Gln
<210> 365
<211> 373
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 365
Met Pro Glu Asn Asn Arg Tyr Tyr Asn Ile Ala Leu Lys Ser Asn Pro
1 5 10 15
Lys Lys Val Trp Asn Val Ser Gln Ala Ser Asn Asp Asn Asp Arg Ala
20 25 30
Ile Val Leu Trp Gln Gly Gly Ser Ala Asp His Glu Arg Phe Ala Phe
35 40 45
Phe Gln Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Val Val Thr Phe Gly Asn Gly Ser Trp Val Ser Gly Gly Pro Ile
65 70 75 80
Pro Ala Asn Glu Thr Leu Gln Gln Gln Lys Trp Thr Gly Glu Ala Lys
85 90 95
Gln Lys Trp Asn Leu Arg Asp Leu Gly Ser Asn Gly Gln Gly Ser Asn
100 105 110
Asn Tyr Glu Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg
115 120 125
Trp Ile Gly Ser Val Val Asp Asn Thr Glu Tyr Leu Gln Leu Asp Asn
130 135 140
Ser Asn Pro Ser Asp Pro Asp Lys Val Phe Thr Leu Phe Asn Gly Thr
145 150 155 160
Leu Asn Ile Phe Gly Gln Asn Met Thr Phe Phe Asn Asp Thr Ile Ser
165 170 175
Val Pro Lys Leu Pro Ala Thr Gly Thr Arg Pro Asp Ala Pro Asn Tyr
180 185 190
Thr Gly Gly Val Asp Gln Gln Leu Pro Gln Val Ser Asp Ser Val Val
195 200 205
Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Asn Asp Asn Gln Ala
210 215 220
Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys
225 230 235 240
Glu Glu Tyr Trp Asp Lys Thr Tyr Ser Ala Val Ile Pro Ala Gly Val
245 250 255
Thr His Asn Tyr Ser Phe Thr Thr Gly Met Ser Ser Val Asp Gln Gln
260 265 270
Lys Met Thr Asp Thr Leu Ser Met Ser Ile Gly Ala Asp Phe Gly Leu
275 280 285
Ser Phe Gly Gly Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr
290 295 300
Ile Gln Thr Glu Ile Thr Ser Thr Asn Thr Asn Ala Ser Glu Glu Lys
305 310 315 320
Val Ala Arg Ala Val Glu Ser Glu Pro Gly Lys Thr Thr Gly Phe Thr
325 330 335
Glu Tyr Gln Leu Ala Thr Arg Tyr Thr Leu Lys Arg Ala Asp Gly Ser
340 345 350
Asn Val Ser Asp Pro Trp Val Val Lys Asn Asn Lys Ile Thr Ile Thr
355 360 365
Arg Lys Asn Ala Gln
370
<210> 366
<211> 380
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 366
Met Ser Asn Glu Asn Ser Ser Lys Glu Asn Leu Asn Thr Pro Leu Ile
1 5 10 15
Asn Asp Glu Asp Leu Ile Pro Val Glu Ile Lys Ala Ser Gly Val Arg
20 25 30
Ile Ser Phe Pro Asp Phe Tyr Phe Tyr Lys Ile Arg Thr Phe Cys Gly
35 40 45
Lys Tyr Ile Asp Ile Gln Asp Ala Ser Thr Ala Asn Asn Lys Ala Ala
50 55 60
Ile Gln Tyr Thr Ala Asn Asn Asn Asn Asn Gln Lys Phe Leu Ile Phe
65 70 75 80
Thr Leu Asp Asn Asn Tyr Ser Val Ile Ala Ala Lys His Ser Gly Lys
85 90 95
Val Leu Asp Ile Met Asp Ile Pro Leu Gly Pro Gly Leu Pro Phe Met
100 105 110
Leu Ile Gln Tyr Asp Phe Gln Asn Gly Asp Asn Gln Lys Phe Phe Ile
115 120 125
Ala Asn Asp Gly Ala Ile Ala Val Lys Lys Thr Gly His Val Trp Asp
130 135 140
Val Lys Ser Gly Ser Thr Lys Asp Asp Thr Pro Ile Ile Pro Tyr Asn
145 150 155 160
Tyr Ser Gly Ala Ser Asn Gln Lys Phe Ser Leu Glu Asn Ser Gly Thr
165 170 175
Ala Ser Val Lys Pro Pro Glu Leu Gly Asp Leu Pro Asn Ser Pro Asp
180 185 190
Phe Lys Thr Asn Asp Leu Asn Glu Ile Leu Pro Asp Asn Thr Thr Pro
195 200 205
Val Ile Thr His Ala Thr Tyr Leu Pro Tyr Phe Met Val Lys Asp Pro
210 215 220
Tyr Tyr Asn Thr Gln Gln Lys Met Gln Asn Ser Pro Tyr Tyr Ile Leu
225 230 235 240
Val Arg Arg Gln Tyr Trp Glu Lys Ile Ser Gln Arg Ile Val Gly Ala
245 250 255
Gly Glu Ser Tyr Lys Tyr Glu Gln Thr Thr Gly Val Ala Arg Thr Asp
260 265 270
Gln Ile Ser Met Thr Glu Thr Thr Glu Ile Ala Ile Gly Ala Asp Leu
275 280 285
Gly Phe Met Phe Lys Gly Phe Ser Ala Asn Leu Ser Thr Ser Ile Thr
290 295 300
Lys Thr Leu Ser Val Thr Lys Ser Thr Ser Thr Thr Glu Ser Val Ser
305 310 315 320
Lys Thr Glu Thr Val Thr Asp Ser Asn Pro Phe Met Tyr Thr Ile Ala
325 330 335
Arg Ala Lys Tyr Met Leu Ile Asn Glu Tyr Tyr Val Thr Arg Ala Asn
340 345 350
Gly Gln Leu Ile Thr Ser Gly Asp Ala Thr Tyr Trp Lys Val Pro Asn
355 360 365
Pro Asn Gln Thr Val Thr Arg Thr Ile Pro Gln Pro
370 375 380
<210> 367
<211> 372
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 367
Met Pro Glu Asn Asn Arg Tyr Tyr Asn Ile Ala Leu Lys Ser Asn Thr
1 5 10 15
Lys Lys Val Trp Asn Val Ser Gln Ala Ser Asn Asp Asn Asp Arg Ala
20 25 30
Ile Val Leu Trp Gln Gly Gly Ser Ala Asp His Glu Arg Phe Ala Phe
35 40 45
Phe Gln Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Val Val Thr Phe Gly Thr Gly Ser Trp Val Ser Gly Gly Pro Ile
65 70 75 80
Pro Ala Ser Glu Thr Leu Gln Gln Gln Lys Trp Thr Gly Glu Ala Lys
85 90 95
Gln Lys Trp Asn Leu Arg Asp Leu Gly Ser Asn Gly Gln Gly Ser Asn
100 105 110
Asn Tyr Glu Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg
115 120 125
Trp Ile Gly Ser Val Val Asp Asn Thr Glu Tyr Leu Gln Leu Asp Asn
130 135 140
Ser Asn Pro Ser Asp Ala Asp Lys Val Phe Thr Leu Phe Asn Gly Thr
145 150 155 160
Met Asn Leu Gly Gly Met Asp Phe Thr Phe Phe Asn Asp Thr Ile Ser
165 170 175
Val Pro Lys Leu Pro Ala Thr Gly Thr Arg Pro Glu Ala Pro Val Tyr
180 185 190
Thr Gly Gly Ala Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val
195 200 205
Val Gly Ala Ser Leu Ile Pro Cys Phe Met Val Asn Asp Asn Gln Ala
210 215 220
Ser Asp Phe Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys
225 230 235 240
Glu Glu Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu
245 250 255
Ser Glu Ser Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln
260 265 270
Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Leu Gly Ile
275 280 285
Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr
290 295 300
Ile Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr
305 310 315 320
Arg Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr
325 330 335
Gln Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser
340 345 350
Ile Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr
355 360 365
Arg Lys Thr Leu
370
<210> 368
<211> 374
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 368
Met Pro Glu Asn Asn Arg Tyr Tyr Asn Ile Ala Leu Lys Ser Asn Ala
1 5 10 15
Lys Lys Val Trp Asn Val Ser Gln Ala Ser Asn Asp Asn Asp Arg Ala
20 25 30
Ile Ile Leu Trp Gln Gly Gly Ser Ala Asp His Glu Lys Phe Ala Phe
35 40 45
Phe Gln Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Val Val Thr Phe Gly Thr Gly Ser Trp Val Ser Gly Gly Pro Ile
65 70 75 80
Pro Ala Ser Glu Thr Leu Gln Gln Gln Ser Trp Thr Gly Glu Ala Lys
85 90 95
Gln Lys Trp Asn Leu Arg Asp Leu Gly Ser Asn Gly Gln Gly Ser Asn
100 105 110
Asn Tyr Glu Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg
115 120 125
Trp Met Gly Ser Ile Val Asp Asn Thr Glu Phe Leu Gln Leu Asp Asn
130 135 140
Ser Asn Pro Ser Asp Ala Asp Lys Val Phe Thr Leu Phe Asn Gly Thr
145 150 155 160
Ile Asn Leu Gly Gly Met Asp Phe Thr Phe Phe Lys Asp Thr Ile Pro
165 170 175
Val Pro Lys Leu Pro Ala Thr Gly Thr Arg Pro Glu Ala Pro Val Tyr
180 185 190
Thr Gly Gly Ala Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val
195 200 205
Val Gly Ala Ser Leu Ile Pro Cys Phe Met Val Lys Asp Asn Gln Ala
210 215 220
Asn Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys
225 230 235 240
Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Ala Gly Tyr
245 250 255
Thr Arg Asn Tyr Thr Phe Lys Ser Gly Met Thr Ser Val Asp Gln Gln
260 265 270
Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu
275 280 285
Lys Phe Glu Gly Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr
290 295 300
Ile Gln Thr Glu Ile Thr Ser Thr Asp Thr Gln Val Ser Glu Glu Thr
305 310 315 320
Val Ala Ser Thr Leu Thr Ser Pro Ala Gly Lys Thr Thr Gly Phe Thr
325 330 335
Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Thr
340 345 350
Leu Val Ser Asp Pro Trp Ile Val Lys Asn Asn Lys Ile Thr Ile Val
355 360 365
Arg Asp Lys Ala Gln Glu
370
<210> 369
<211> 358
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 369
Met Lys Arg Tyr Lys Lys Leu Leu Met Val Ala Pro Leu Ala Cys Met
1 5 10 15
Leu Gly Thr Gly Val Val Thr Leu Pro Thr Val Ser His Ala Asp Ala
20 25 30
Ala Pro Val Ser Ser Val Val Asn Asp Val Phe Arg Gln Gly Ser Thr
35 40 45
Tyr Ser Thr Glu Asn Ile Thr Gln Ala Leu Tyr Val Arg Leu Arg Asp
50 55 60
Gly Ile Glu Ala Ser Pro Glu Leu Arg Gln Lys Phe Lys Ile Ala Asp
65 70 75 80
Asp Glu Lys Ile Gln Phe Glu Ala Asp Ala Gly Asn Phe Asn Gly Val
85 90 95
Lys Thr Pro Ala Ile Asn Tyr Gly Leu Arg Asp Gln Leu Ser Lys Val
100 105 110
Thr Ala Ser Ser Ile Gly Lys Asn Ile Ser Val Asn Pro Thr Thr Val
115 120 125
Thr Glu Val Glu Ala Gly Lys Ile Glu Leu Leu Thr Tyr Arg Asn Glu
130 135 140
Thr Pro Leu Thr Gln Lys Gln Tyr Thr Thr Glu Lys Ser Val Lys Arg
145 150 155 160
Ile Asp Thr Val Thr Ala Thr Asn Gln Tyr Gly Phe Lys Leu Gly Phe
165 170 175
Glu Ser Asn Thr Glu Phe Lys Val Asp Ala Leu Ile Ala Ser Ala Lys
180 185 190
Gln Ser Phe Lys Ala Ser Ala Glu Phe Ser Phe Ser His Thr Asp Thr
195 200 205
Asn Thr Val Thr Asn Glu Glu Thr Val Thr Phe Lys Ala Gln Glu Val
210 215 220
Leu Ala Ala Ser Gly Gly Thr Thr Asn Tyr Phe Thr Arg Val Gly Lys
225 230 235 240
Ala Lys Phe Ser Gly Ser Phe Gln Thr Asp Ala Ala Leu Thr Asp Leu
245 250 255
Lys Leu Lys Leu Pro Ile Thr Lys Lys Asn Gln Asn Gln Thr Val Ala
260 265 270
His Thr Glu Glu Ala Thr Leu Thr Ser Gln Asp Ile Tyr Thr Ile Phe
275 280 285
Lys Asn Thr Pro Tyr Ala Pro Leu Pro Pro Tyr Leu Ser Phe Asp Asp
290 295 300
Asn Gly Lys Lys Val Leu Val Lys Asn Val Thr Phe Asp Tyr Thr Gly
305 310 315 320
Glu Met Gly Tyr Asn Thr Ala Ala Gln Ala Glu Phe Ile Pro Phe Asp
325 330 335
Gln Glu Lys Pro Thr Gln Thr Met Ser Tyr Glu Lys Tyr Glu Ala Lys
340 345 350
Ala Gln Asp Lys Ser Leu
355
<210> 370
<211> 335
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 370
Met Pro Thr Val Ser His Ala Asp Ala Ala Pro Val Ser Ser Val Val
1 5 10 15
Asn Asp Val Phe Arg Gln Gly Ser Thr Tyr Ser Thr Glu Asn Ile Thr
20 25 30
Gln Ala Leu Tyr Val Arg Leu Arg Asp Gly Ile Glu Ala Ser Pro Glu
35 40 45
Leu Arg Gln Lys Phe Lys Ile Ala Asp Asp Glu Lys Ile Gln Phe Glu
50 55 60
Ala Asp Ala Gly Asn Phe Asn Gly Val Lys Thr Pro Ala Ile Asn Tyr
65 70 75 80
Gly Leu Arg Asp Gln Leu Ser Lys Val Thr Ala Ser Ser Ile Gly Lys
85 90 95
Asn Ile Ser Val Asn Pro Thr Thr Val Thr Glu Val Glu Ala Gly Lys
100 105 110
Ile Glu Leu Leu Thr Tyr Arg Asn Glu Thr Pro Leu Thr Gln Lys Gln
115 120 125
Tyr Thr Thr Glu Lys Ser Val Lys Arg Ile Asp Thr Val Thr Ala Thr
130 135 140
Asn Gln Tyr Gly Phe Lys Leu Gly Phe Glu Ser Asn Thr Glu Phe Lys
145 150 155 160
Val Asp Ala Leu Ile Ala Ser Ala Lys Gln Ser Phe Lys Ala Ser Ala
165 170 175
Glu Phe Ser Phe Ser His Thr Asp Thr Asn Thr Val Thr Asn Glu Glu
180 185 190
Thr Val Thr Phe Lys Ala Gln Glu Val Leu Ala Ala Ser Gly Gly Thr
195 200 205
Thr Asn Tyr Phe Thr Arg Val Gly Lys Ala Lys Phe Ser Gly Ser Phe
210 215 220
Gln Thr Asp Ala Ala Leu Thr Asp Leu Lys Leu Lys Leu Pro Ile Thr
225 230 235 240
Lys Lys Asn Gln Asn Gln Thr Val Ala His Thr Glu Glu Ala Thr Leu
245 250 255
Thr Ser Gln Asp Ile Tyr Thr Ile Phe Lys Asn Thr Pro Tyr Ala Pro
260 265 270
Leu Pro Pro Tyr Leu Ser Phe Asp Asp Asn Gly Lys Lys Val Leu Val
275 280 285
Lys Asn Val Thr Phe Asp Tyr Thr Gly Glu Met Gly Tyr Asn Thr Ala
290 295 300
Ala Gln Ala Glu Phe Ile Pro Phe Asp Gln Glu Lys Pro Thr Gln Thr
305 310 315 320
Met Ser Tyr Glu Lys Tyr Glu Ala Lys Ala Gln Asp Lys Ser Leu
325 330 335
<210> 371
<211> 327
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 371
Met Ala Pro Val Ser Ser Val Val Asn Asp Val Phe Arg Gln Gly Ser
1 5 10 15
Thr Tyr Ser Thr Glu Asn Ile Thr Gln Ala Leu Tyr Val Arg Leu Arg
20 25 30
Asp Gly Ile Glu Ala Ser Pro Glu Leu Arg Gln Lys Phe Lys Ile Ala
35 40 45
Asp Asp Glu Lys Ile Gln Phe Glu Ala Asp Ala Gly Asn Phe Asn Gly
50 55 60
Val Lys Thr Pro Ala Ile Asn Tyr Gly Leu Arg Asp Gln Leu Ser Lys
65 70 75 80
Val Thr Ala Ser Ser Ile Gly Lys Asn Ile Ser Val Asn Pro Thr Thr
85 90 95
Val Thr Glu Val Glu Ala Gly Lys Ile Glu Leu Leu Thr Tyr Arg Asn
100 105 110
Glu Thr Pro Leu Thr Gln Lys Gln Tyr Thr Thr Glu Lys Ser Val Lys
115 120 125
Arg Ile Asp Thr Val Thr Ala Thr Asn Gln Tyr Gly Phe Lys Leu Gly
130 135 140
Phe Glu Ser Asn Thr Glu Phe Lys Val Asp Ala Leu Ile Ala Ser Ala
145 150 155 160
Lys Gln Ser Phe Lys Ala Ser Ala Glu Phe Ser Phe Ser His Thr Asp
165 170 175
Thr Asn Thr Val Thr Asn Glu Glu Thr Val Thr Phe Lys Ala Gln Glu
180 185 190
Val Leu Ala Ala Ser Gly Gly Thr Thr Asn Tyr Phe Thr Arg Val Gly
195 200 205
Lys Ala Lys Phe Ser Gly Ser Phe Gln Thr Asp Ala Ala Leu Thr Asp
210 215 220
Leu Lys Leu Lys Leu Pro Ile Thr Lys Lys Asn Gln Asn Gln Thr Val
225 230 235 240
Ala His Thr Glu Glu Ala Thr Leu Thr Ser Gln Asp Ile Tyr Thr Ile
245 250 255
Phe Lys Asn Thr Pro Tyr Ala Pro Leu Pro Pro Tyr Leu Ser Phe Asp
260 265 270
Asp Asn Gly Lys Lys Val Leu Val Lys Asn Val Thr Phe Asp Tyr Thr
275 280 285
Gly Glu Met Gly Tyr Asn Thr Ala Ala Gln Ala Glu Phe Ile Pro Phe
290 295 300
Asp Gln Glu Lys Pro Thr Gln Thr Met Ser Tyr Glu Lys Tyr Glu Ala
305 310 315 320
Lys Ala Gln Asp Lys Ser Leu
325
<210> 372
<211> 343
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 372
Met Asn Glu Val Leu Gly Asn Ile Gly Gln Asn Pro Gln Val Ser Lys
1 5 10 15
Arg Ala Leu Arg Ser Arg Arg Ser Pro Val Val Phe Ile Asn Ala Thr
20 25 30
Pro Glu Glu Ala Glu Gln Phe Gln Glu Ile Val Ala Ile Asp Asp Ile
35 40 45
Asp Tyr Leu Thr Gln Ala Val Ala Leu Thr Ala Leu Phe Asn Gly Ala
50 55 60
Ile Asp Asn Thr Thr Gly Arg Phe Ile Pro Gly Lys Ala Arg Glu Leu
65 70 75 80
Ile Ala Asn Tyr Asn Glu Ser Leu Asp Ser Asp Ser Gln Arg Tyr Lys
85 90 95
Ile Gly Ile Phe Asn Thr His Gln Thr Thr Leu Thr Gln Gln Asn Ser
100 105 110
Ala Val Ser Ala Met Ile Asn Gln Ile Leu Glu Thr Leu Lys Asn Val
115 120 125
Leu Gly Val Ala Leu Gly Thr Thr Ser Val Ala Gln Met Thr Ala Ala
130 135 140
Ile Thr Asp Ala Phe Thr Asn Leu Asp Glu Gln Ser Gly Asp Ala Trp
145 150 155 160
Ile Phe Trp Glu Lys Lys Thr Ser Asn Lys Thr Thr Tyr Ser Tyr Ala
165 170 175
Ile Leu Phe Ala Phe Gln Asp Ser Ser Thr Gly Lys Leu Met Phe Ala
180 185 190
Leu Pro Met Ser Leu Glu Ile Glu Val Asp Val Arg Tyr Glu Arg Val
195 200 205
Leu Phe Ile Thr Val Glu Asp Lys Glu Thr Tyr Ser Val Lys Leu Asp
210 215 220
Thr Met Lys Val Gly Gln Leu Leu Phe Pro Lys Ser Pro Gly Ala Asn
225 230 235 240
Ala Leu Gln Thr Ala Arg Arg Leu Gly Val Arg Ser Ala Lys Ala Asp
245 250 255
Leu Leu Ala Ala Pro Ala Pro Ile Ser Asn Ile Val Val Thr Asn Trp
260 265 270
Ala Lys Thr Thr Thr Tyr Ala Thr Ala Ala Thr Gly Val Tyr Thr Glu
275 280 285
Ser His Pro Leu Val Gln Val Met Ala Val His Pro Asn Val Ser Asn
290 295 300
Pro Leu Gln Asp Gly Asn Asn Tyr Leu Val Ser Phe Thr Ile Asn Gly
305 310 315 320
Val Gln Gln Thr Arg Gly Leu Leu Leu Asn Gly Ile Met Pro Asp Gly
325 330 335
Glu Leu Trp Phe Val Ser Ile
340
<210> 373
<211> 351
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 373
Met Ile Gly Ile Glu Gln Ser Asn Thr Glu Asn Lys Glu Leu Ser Gly
1 5 10 15
Asn Thr Asp Lys Met Lys Gln Thr Leu Val Lys Ala Gly Ala Phe Ala
20 25 30
Gln Ala Met Asn Leu Tyr Ser Tyr Met Leu Leu Lys Asn Pro Asp Val
35 40 45
Asn Phe Ala Gly Leu Asp Pro Asn Thr Asp Leu Ala Gly His Gly Asp
50 55 60
Leu Pro Gly Lys Leu Leu Glu Ser Gln Arg Gln Ala Arg Ala His Ala
65 70 75 80
Ser Thr Trp Asp Lys Thr Val Lys Arg Gln Leu Leu Asp Thr Leu Thr
85 90 95
Gly Ile Ile Thr Phe Asp Thr Thr Phe Gln Ala Tyr Tyr Gln Thr Leu
100 105 110
Val Asp Ser Val Asp Lys Gly Asp Lys Glu Thr Leu Lys Glu Gly Ile
115 120 125
Lys Asp Leu Gln Arg Asp Val Gln Lys Asn Lys Lys Cys Ala Val Ser
130 135 140
Leu Leu Asp Ala Leu Lys Asp Leu Lys Thr Ala Ile Asp Glu Asp Asn
145 150 155 160
Arg Ser Phe Gln Glu His Gln Thr Thr Leu Ser Ser Ile Leu Lys Gly
165 170 175
Lys Thr Ala Asp Thr Gly Ile Glu Ala Asp Glu Ala Asn Leu Lys Lys
180 185 190
Ile Met Glu Asp Ile Asn Tyr Tyr Arg Lys Tyr Gln Met Asp Leu Ser
195 200 205
Pro Leu Ser Ile Leu Gln Trp Ile Ile Ser Pro Ile Gly Ala Gly Val
210 215 220
Ser Val Ala Val Ala Lys Thr His Leu Asp Lys Leu Arg Pro Ala Phe
225 230 235 240
Glu Glu Leu Asn Lys Met Ile Asp Gln Lys Arg Thr Val Asn Arg Ile
245 250 255
Val Phe Thr Ala Ala Gln Asn Val Asn Glu Leu Ala Asn Ala Ile Asp
260 265 270
Gly Ala Thr Gln Ser Leu Gln Tyr Met Val Asp Gln Trp Asn Asp Leu
275 280 285
Asp Ser Gln Tyr Asp Gly Val Leu Asp Ser Ile Glu Thr Ala Asp Ala
290 295 300
Lys Ile Gln Glu Asp Arg Phe Lys Phe Leu Lys Pro Thr Leu Lys Thr
305 310 315 320
Ala Lys Asp Asn Trp Asn Ala Leu Lys Thr Asp Ala Asp Thr Leu Lys
325 330 335
Glu Gly Leu Lys Glu Leu Lys Ile Glu Pro Ala Asn Thr Gln Asn
340 345 350
<210> 374
<211> 344
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 374
Met Asn Glu Val Leu Ala Asn Asn Ile Gly Gln His Pro Gln Val Ser
1 5 10 15
Arg Arg Ala Leu Arg Ser Pro Arg Ser Pro Val Val Phe Ile Asn Thr
20 25 30
Thr Ser Glu Glu Ala Glu Gln Phe Gln Glu Ile Val Ala Ile Asp Asp
35 40 45
Ile Asp Tyr Leu Thr Gln Ala Val Ala Leu Thr Ala Leu Phe Asn Gly
50 55 60
Ala Ile Asp Asn Thr Thr Gly Arg Phe Ile Pro Gly Arg Ala Arg Glu
65 70 75 80
Leu Ile Ala Asn Tyr Asn Glu Ser Leu Asp Pro Glu Ser Gln Arg Tyr
85 90 95
Lys Ile Gly Ile Phe Asn Thr His Gln Thr Thr Leu Thr Gln Gln Asn
100 105 110
Ser Ala Val Ser Ala Met Ile Asn Gln Ile Leu Glu Thr Leu Lys Asn
115 120 125
Val Met Gly Val Ala Leu Gly Thr Thr Ser Val Thr Gln Met Thr Ala
130 135 140
Ala Ile Thr Asp Ala Phe Thr Asn Leu Asn Glu Gln Ser Gly Asp Ala
145 150 155 160
Trp Ile Phe Trp Glu Lys Lys Thr Ser Asn Lys Thr Thr Tyr Ser Tyr
165 170 175
Ala Ile Leu Phe Ala Phe Gln Asp Ser Ser Thr Gly Lys Leu Met Phe
180 185 190
Ala Leu Pro Met Ser Leu Glu Ile Glu Val Asp Val Ser Tyr Glu Arg
195 200 205
Val Leu Phe Ile Thr Val Glu Asp Lys Glu Thr Tyr Ser Val Lys Leu
210 215 220
Asp Thr Met Lys Val Gly Gln Leu Leu Phe Pro Lys Ser Pro Gly Ala
225 230 235 240
Asn Ala Leu Gln Thr Val Arg Arg Leu Arg Thr Arg Ser Gly Ser Ser
245 250 255
Gly Leu Leu Thr Ala Pro Ser Pro Val Thr Asn Ile Val Val Thr Asn
260 265 270
Trp Ala Lys Thr Thr Thr Phe Ala Thr Ser Ala Asn Gly Phe Tyr Thr
275 280 285
Glu Asn His Pro Leu Val Gln Leu Met Ala Val Gly Pro Thr Val Val
290 295 300
Asn Pro Leu Tyr Asp Gly Asn Gln Tyr Leu Val Ser Phe Asp Leu Asn
305 310 315 320
Gly Gly Arg Gln Thr Arg Gly Leu Ile Leu Asn Gly Thr Leu Pro Asp
325 330 335
Gly Glu Leu Trp Phe Val Ser Met
340
<210> 375
<211> 607
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 375
Met Lys Tyr Lys Asp Arg Lys Asn Ala Lys Arg Lys Tyr Lys Gln Ala
1 5 10 15
Leu Leu Ala Thr Val Ala Thr Met Thr Leu Gly Val Ser Thr Leu Gly
20 25 30
Ser Thr Ala Ser Val Phe Ala Glu Glu Asn Thr Gln Gln Gln Gln Ser
35 40 45
Ala Met Ala Pro Ala Thr Asp Gly Ser Val Ile Asn Ile Asp Gly Ser
50 55 60
Gly Asp Ile Lys Thr Asp Gly Ile Val Phe Lys Lys Glu Ser Thr Asp
65 70 75 80
Gly Leu Val Phe Lys Arg Ala Asp Asp Gly Thr Ile Thr Val Ser Lys
85 90 95
Glu Lys Glu Lys Leu Phe Asn Ile Asn Thr Leu Lys Ala Ile Gly Ser
100 105 110
Leu Gly Gly Ala Thr Phe Lys Gln Ala Trp Ala Asp Arg Lys Ala Gly
115 120 125
Asn Phe Asn Asn Thr Phe Arg Thr Leu Ala Met Gly Ser Ala Ala Leu
130 135 140
Ile Pro Tyr Gly Gly Val Val Ile Ser Pro Leu Ile Gly Tyr Leu Trp
145 150 155 160
Pro Glu Asn Asp His Glu Ala Gln Ala Lys Leu Lys Gln Leu Met Ser
165 170 175
Asp Ile Ala Ala Gln Thr Lys Thr Gln Ile Ala Asp Tyr Asp Val Glu
180 185 190
Ala Leu Gly Ser Lys Leu Lys Lys Leu Arg Lys Asp Leu Gln Glu Phe
195 200 205
Glu Gly Ile Met Asn Pro Thr Gly Arg Lys Ile Ala Ala Ala Pro Ser
210 215 220
Val Gly Asp Glu Ala Glu Ser Thr Arg Ile Leu Val Gly Asn Ile His
225 230 235 240
Asn Asp Phe Lys Asn Leu Ile Glu Glu Ser Gln Lys Glu Ser Tyr Lys
245 250 255
Glu Thr Glu Leu Pro Ile Phe Met Val Ala Ala Thr Ala His Leu Gln
260 265 270
Phe Leu Asn Tyr Leu Ala Leu His Gly Lys Asp Pro Lys Leu Asn Tyr
275 280 285
Asp Asp Lys Gly Leu Lys Ile Ala Ala Leu Thr Pro Leu Glu Arg Ala
290 295 300
His Lys Glu Tyr Lys Glu Tyr Ala Thr Glu Ala Ala Lys Lys Gly Leu
305 310 315 320
Asp Ala Ile Asn Asn Ala Glu Arg Pro Val Lys Gln Met Gln Glu Ser
325 330 335
Trp Thr Phe Lys Tyr Leu Ala Ser Asp Leu Lys Lys Gln Ala Leu Ser
340 345 350
Ala Thr Ile Met Gly Pro Asp Thr Val Val Lys Glu Thr Phe Asp Thr
355 360 365
Thr Ile Asn Asn Ile Ala Phe Lys Gln Ala Leu Gly Ile Lys Asp Asp
370 375 380
Trp Thr Met Asp Gln Asp Gly Arg Ile Leu Tyr Tyr Asp Pro Gln Asp
385 390 395 400
His Lys Met Gln Thr Gly Trp Lys Glu Ile Ser Thr Ser Phe Ala Ala
405 410 415
Arg Ile Gln Asn Thr Gly Asn Leu Ser Ile Gln Phe Leu Lys Asp Gln
420 425 430
Gly Leu Lys Tyr Ser Trp Tyr Tyr Phe Ser Pro Glu Lys Thr Asp Lys
435 440 445
Phe Ala Lys Gly Glu Leu Tyr Lys Asp Thr Thr Glu Thr Ile Asn Gly
450 455 460
Lys Thr Tyr Thr Phe Gly Val Asp Gly Thr Cys Leu Asn Pro Asp Gly
465 470 475 480
Gly Glu Ala Ala Gly Trp His Gln Ile Gly Glu Ala Trp Tyr Phe Tyr
485 490 495
Ser Asp Gly Lys Thr Lys Asn Ser Glu Gly Lys Thr Phe Ala Lys Asn
500 505 510
Glu Lys Val Thr Gly Trp Tyr Lys Asp Glu Lys Ala Gly Arg Tyr Tyr
515 520 525
Tyr Leu Ser Pro Gly Glu Thr Lys Asn Ser Asp Lys Lys Val Phe Lys
530 535 540
Ala Gly Glu Met Val Thr Gly Trp Tyr Gln Glu Gly Asn Lys Trp Tyr
545 550 555 560
Tyr Leu Thr Pro Asn Pro Arg Lys Asn Lys Glu Gly Ser Glu Phe Ala
565 570 575
Ala Gly Gln Leu Ile Phe Gly Thr Leu Ser Ile Glu Asp Lys Asn Gly
580 585 590
Glu Tyr Val Gln Tyr Lys Phe Asn Asn Asp Gly Ser Leu Lys Glu
595 600 605
<210> 376
<211> 369
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 376
Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Ser Gly Asn Gln Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Tyr Leu Ala Asp Ala Pro Ser Glu Arg Phe Phe
35 40 45
Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Ile Ser Gly Asn Gln Thr Gly Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ala Pro Ser Glu Tyr Trp Tyr
85 90 95
Phe His Arg Glu Ala Asp Gly Tyr Tyr Val Ile Glu Ser Lys His Ser
100 105 110
Gly Lys Val Leu Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
115 120 125
Val Gln Gln Phe Glu Phe Leu Ala Asp Ala Pro Ser Glu Arg Phe Ala
130 135 140
Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile Asn Thr Gln Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp Gln Leu Pro Glu
165 170 175
Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val Pro Ala Ile Ser
180 185 190
Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Glu
210 215 220
Ser Tyr Val Leu Ala Pro Ser Glu Arg Tyr Asp Phe Val Thr Thr Thr
225 230 235 240
Gly Ile Lys Val Thr Asp Gln Glu Thr Ala Thr Lys Thr Val Ser Trp
245 250 255
Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly Phe Ser Leu Gly
260 265 270
Met Ser Ser Gln Tyr Ser Gln Glu Leu Gln Thr Ser Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Thr Gln Glu His His Ile Thr Asn Pro
290 295 300
Phe Val Glu Arg Met Ala Tyr Ser Arg Tyr Ile Leu Thr Thr Glu Tyr
305 310 315 320
Tyr Val Gln Arg Lys Asn Gly Thr Ile Val Asn Ala Pro Trp Thr Met
325 330 335
Thr Asp Lys Thr Asn Ala His Ala Val Thr Phe Pro Lys Ser Thr Gly
340 345 350
Lys Val Leu Asn Glu Asn Ile Lys Gln Ile Ser Lys Ser Glu Ser Leu
355 360 365
Asn
<210> 377
<211> 363
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 377
Met Ala Phe Asn Leu Gly Ser Tyr Tyr Lys Ile Asn Cys Lys Ile Ser
1 5 10 15
Gly Lys Val Phe Asp Val Arg Asp Glu Ser Leu Asn Asn Gly Ala Tyr
20 25 30
Ile Gln Gln Tyr Thr Trp Lys Asn Ile Asp Gly Gln Lys Phe Met Val
35 40 45
Tyr Pro Leu Asp Asn Gly Thr Tyr Ala Phe Ala Ser Lys Leu Ser Gly
50 55 60
Lys Val Phe Ser Val Arg Ala Ala Ser Met Asp Asn Gln Ala Val Ile
65 70 75 80
Glu Gln Tyr Asp Trp Ser Ala Glu Asp Lys Gln Lys Phe Tyr Leu Thr
85 90 95
Leu Gln Glu Ala Asn Tyr Tyr Ser Gly Thr Ser Gln Leu Ser Gly Lys
100 105 110
Val Trp Asp Val Arg Asp Ser Ser Thr His Asn Gln Ala Leu Ile Gln
115 120 125
Gln Tyr Thr Trp Lys Asp Ile Asp Gly Gln Lys Phe Ser Phe Thr Pro
130 135 140
Val Asp Thr Ile Ser Val Pro Ser Thr Thr Thr Thr Pro Leu Pro Gly
145 150 155 160
Val Pro Glu Tyr His Gly Tyr Asn Asp Gln Leu Pro Asp Gln Thr Tyr
165 170 175
Pro Val Val Thr Ala Tyr Thr Val Ala Pro Phe Phe Ala Ile Ser Asp
180 185 190
Pro Arg Tyr Gly Thr Asn Thr Ala Arg Gln Val Lys Glu Asn Pro Tyr
195 200 205
Tyr Leu Tyr Ile Arg Lys Glu Tyr Trp Lys Leu Leu Gln Ser His Thr
210 215 220
Phe Ala Pro Gly Glu Asp Ala Thr Phe Val Tyr Arg Thr Gly Ile Ser
225 230 235 240
Thr Val Asp Gln Thr Ala Ser Ser Thr Thr Thr Ser Thr Thr Ile Gly
245 250 255
Ala Asp Gly Gly Leu Gln Phe Lys Lys Leu Ser Val Gly Leu Ser Ser
260 265 270
Gln Tyr Thr Thr Glu Leu Gln Thr Ser Gln Ser Thr Thr Asp Ala Glu
275 280 285
Met Lys Glu Gln Glu Val Ala Ile Thr Leu Thr Asn Ile His Pro Tyr
290 295 300
Pro Val Ala Trp Ser Lys Tyr Ile Leu Val Arg Glu Tyr His Val Glu
305 310 315 320
Arg Ser Asp Gly Ser Ile Val Asn Thr Pro Trp Thr Val Ser Leu Pro
325 330 335
Asn Arg Thr Gln Ser Val Tyr Tyr Pro Asn Glu Glu Met Leu Arg Arg
340 345 350
Pro Val Leu Ile Glu Phe Glu Asn Tyr Ser Leu
355 360
<210> 378
<211> 371
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 378
Met Met Ser Asn Glu Asn Asn Asn Arg Arg Asn Met Asn Ala Ser Met
1 5 10 15
Gly Ser Asp Gly Glu Ser Ile Gln Asn Lys Ile Gly Asp Ser Arg Ser
20 25 30
Gln Ile Ser Phe Pro Asp Phe Asn Phe Tyr Lys Ile Arg Thr Phe Ser
35 40 45
Gly Lys Tyr Ala Asp Ile Thr Gly Asp Ser Leu Leu Val Gln Tyr Asn
50 55 60
Gly His Asn Gly Asp Asn Gln Lys Phe Leu Ile Val Thr Leu Asp Asn
65 70 75 80
Asp Tyr Ser Ile Ile Ala Ala Lys His Thr Gly Lys Val Met His Glu
85 90 95
Phe Tyr Leu Phe Gly Thr Gly Arg Phe Leu Gln Ala Asp Phe His Asn
100 105 110
Met Asp Glu Gln Lys Phe Phe Ile Ser Asp Asp Gly Thr Ile Ala Val
115 120 125
Lys Lys Asp Arg Lys Val Trp Asp Val Thr Gly Gly Thr Ser Asn Gly
130 135 140
Ser Pro Ile Ile Pro Tyr Asp Tyr His Gly Ala Ser Asn Gln Lys Phe
145 150 155 160
Thr Leu Glu Arg Ser Gly Ser Val Ser Val Thr Lys Pro Thr Ile Gly
165 170 175
Thr Leu Pro Pro Ala Pro Asp Phe Lys Thr Thr Asp Leu Asn Glu Ile
180 185 190
Leu Pro Asp Asn Thr Glu Pro Val Ile Thr His Ala Thr Tyr Leu Pro
195 200 205
Tyr Phe Met Val Lys Asp Pro Tyr Tyr Asn Asn Gln Gln Lys Met Gln
210 215 220
Asn Ser Pro Tyr Tyr Ile Leu Val Arg Arg Gln Tyr Trp Glu Lys Ile
225 230 235 240
Thr Gln Arg Ile Val Gly Ala Gly Glu Ser Tyr Lys Tyr Glu Gln Lys
245 250 255
Thr Gly Val Ser Arg Thr Asp Gln Thr Ser Met Thr Glu Thr Thr Glu
260 265 270
Ile Ala Ile Gly Ala Asp Met Gly Phe Met Phe Lys Gly Phe Ser Val
275 280 285
Gly Leu Ser Thr Ser Ile Thr Lys Thr Leu Ser Val Thr Lys Ser Thr
290 295 300
Ser Thr Thr Glu Ser Thr Glu Lys Thr Glu Thr Val Thr Asp Ser Asn
305 310 315 320
Pro Phe Met His Thr Ile Ala Arg Ala Lys Tyr Met Leu Ile Asn Glu
325 330 335
Tyr Tyr Val Thr Arg Ala Asn Gly Glu Met Ile Thr Ser Gly Asp Ala
340 345 350
Ala Tyr Trp Lys Val Pro Asn Pro Ser Gln Thr Val Thr Arg Thr Ile
355 360 365
Pro Arg Thr
370
<210> 379
<211> 383
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 379
Met Pro Glu Asn Asn Arg Tyr Tyr Ser Ile His Leu Gln Ser Asn Asn
1 5 10 15
Asn Lys Val Trp Asp Val Phe Asn Asn Ala Thr Tyr Asp Asn Ala Gly
20 25 30
Ile Arg Leu Tyr Gly Gln Thr Asn Gly Asp Asn Gln Gln Phe Val Phe
35 40 45
Phe Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Pro Val Gly Ile Gly Asp Pro Ile Ile Ile Asp Asp Gly Val Arg
65 70 75 80
Arg Met Thr Ala Phe Asn Asn Glu Gly Leu Arg Gln Thr Ser Trp Thr
85 90 95
Gly Ala Pro Ala Glu Gln Trp Tyr Leu Arg Glu Gln Gly Thr Ser Asn
100 105 110
Tyr Glu Phe Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Trp
115 120 125
Met Gly Thr Leu Ala Gln Asn Trp Glu Tyr Val Asp Leu Asp Asn Ser
130 135 140
Asn Pro Thr Asp Gly Asp Arg Val Phe Tyr Val Ser Ala Gly Gln Val
145 150 155 160
Asn Lys Gly Asn Val Pro Phe His Gly Thr Phe Ser Leu Pro Lys Leu
165 170 175
Pro Ala Val Gly Thr Arg Pro Asn Ala Pro Asp Tyr Asn Thr Ser Ala
180 185 190
Gly Pro Asn Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly
195 200 205
Ala Ser Leu Val Pro Cys Ile Met Val Asn Asp Asn Gln Ala Ser Asp
210 215 220
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Ile Lys Glu Glu
225 230 235 240
Tyr Trp Asp Gln Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu
245 250 255
Ser Tyr Thr Phe Lys Ser Gly Val Ser Ser Val Asp Gln Gln Lys Met
260 265 270
Thr Asp Thr Leu Asn Met Lys Ile Gly Ala Asp Phe Gly Leu Gln Phe
275 280 285
Gly Ser Lys Thr Pro Ala Thr Pro Gly Thr Pro Glu Ser Ser Asn Gly
290 295 300
Gly Thr Val Ala Ile Lys Ala Glu Ile Ser Arg Thr Leu Gln Thr Glu
305 310 315 320
Ile Ser Thr Thr Asn Thr Gln Ala Thr Glu Glu Thr Ile Thr Gln Thr
325 330 335
Ala Thr Gly Glu Ala Gly Lys Ala Thr Gly Tyr Thr Gln Tyr Gln Leu
340 345 350
Ala Thr Lys Tyr Thr Leu Lys Arg Ser Asp Gly Ser Ile Val Ser Asp
355 360 365
Pro Trp Val Val Lys Asn Asn Arg Val Thr Val Thr Arg Lys Ile
370 375 380
<210> 380
<211> 338
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 380
Met Ala Glu Gln Thr Pro Ile Asn Thr Ile Ser Glu Glu Gln Lys Ala
1 5 10 15
Ile Ala Pro Phe Lys Glu Ser Tyr Ile Asp Thr Val Gln Asn Arg Met
20 25 30
Lys Ala Arg Asp Ala Glu Ser Lys Arg Thr Gly Lys Ala Ile Asn Leu
35 40 45
Gln Glu Gln Ile Val Asp Gly Trp Phe Leu Ala Arg Phe Trp Ile Phe
50 55 60
Lys Asp Gln Asn Gly His Gln Ala Asn Arg Phe Ile Ser Trp Phe Lys
65 70 75 80
Ala Asn Leu Ala Ser Pro Lys Gly Tyr Asp Ser Val Val Glu Gln Met
85 90 95
Gly Leu Gln Met Glu Ala Leu Asp Asp Met Asp Val Ser Asn Val Asn
100 105 110
Tyr Ala Ser Ser Lys Gly Glu Thr Ile Tyr Asn Gly Glu Ser Thr Leu
115 120 125
Lys Asn Ile Ser Gly Thr Thr Gln Lys Met Lys Thr Asp Ser Phe Gln
130 135 140
Lys Asp Tyr Thr Gln Thr Gln Ser Thr Ser Val Thr Asn Gly Ile Gln
145 150 155 160
Ile Gly Tyr Lys Ile Ser Val Ser Ala Asp Val Pro Leu Met Lys Glu
165 170 175
Asn Ile Glu Ile Ser Thr Thr Tyr Ile Leu Ser Thr Thr Gly Thr Glu
180 185 190
Thr Asn Thr Ile Ser Glu Lys Tyr Asn Val Pro Ser Gln Glu Val Leu
195 200 205
Val Pro Pro Gly His Thr Ala Val Val Lys His Asp Leu Arg Lys Met
210 215 220
Val Tyr Ser Gly Thr His Asp Leu Lys Gly Asp Leu Asn Val Arg Phe
225 230 235 240
Asp Asp Lys Glu Ile Val Gln Lys Phe Val Tyr Pro Asn Tyr Lys Pro
245 250 255
Ile Asn Leu Ser Asp Ile Arg Ser Thr Met Asn Ala Ile Asp Thr Trp
260 265 270
Asn Gly Ile Lys Pro Val Asp Phe Tyr Gln Leu Leu Gly Ile Lys Asn
275 280 285
His Ile Lys Gly Ser Asn Thr Leu Tyr Ile Asp Thr Pro Ala Lys Phe
290 295 300
Lys Phe Asn Gly Ala Ser Pro Tyr Tyr Arg Ala Thr Ile Thr Glu Tyr
305 310 315 320
Asp Lys Asn Gly Asn Pro Val Glu Thr Arg Leu Leu Asp Pro Asn His
325 330 335
Gln Leu
<210> 381
<211> 403
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 381
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ile Ser Glu Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Asn Ile Ala Leu Lys Ser Asn Thr Lys Lys Val
35 40 45
Trp Asn Val Ser Gln Gly Ser Asn Asp Asn Asp Arg Ala Ile Val Leu
50 55 60
Trp Gln Gly Gly Ser Ala Asp His Glu Arg Phe Ala Phe Phe Gln Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Val Val
85 90 95
Thr Phe Gly Thr Gly Ser Trp Val Ser Gly Gly Pro Ile Pro Asp Ser
100 105 110
Glu Thr Leu Gln Gln Gln Lys Trp Thr Gly Glu Ala Lys Gln Lys Trp
115 120 125
Asn Leu Arg Asp Leu Gly Ser Asp Gly His Gly Ser Asn Asn Tyr Glu
130 135 140
Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg Trp Ile Gly
145 150 155 160
Ser Thr Val Asp Asn Thr Glu Tyr Leu Lys Leu Asp Asn Ser Asn Pro
165 170 175
Ser Asp Ala Asp Lys Val Phe Thr Leu Phe Asn Gly Thr Met Asn Leu
180 185 190
Trp Gly Gln Asn Ile Thr Phe Phe Asn Asp Thr Ile Ser Val Pro Lys
195 200 205
Leu Pro Ala Ile Gly Thr Arg Pro Glu Ala Pro Val Tyr Thr Gly Gly
210 215 220
Ala Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val Gly Ala
225 230 235 240
Ser Leu Ile Pro Cys Phe Met Val Asn Asp Asn Gln Ala Ser Asp Tyr
245 250 255
Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu Tyr
260 265 270
Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Gly Gly Leu Lys Arg Asp
275 280 285
Tyr Thr Phe Lys Ser Gly Met Thr Ser Val Asp Gln Lys Lys Met Thr
290 295 300
Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu Lys Phe Gly
305 310 315 320
Asp Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr Leu Gln Thr
325 330 335
Glu Ile Thr Ser Thr Asp Thr Glu Val Ser Glu Glu Thr Val Ser Ser
340 345 350
Ser Leu Thr Ser Pro Ala Gly Lys Thr Thr Gly Phe Thr Glu Tyr Gln
355 360 365
Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val Ser
370 375 380
Asp Pro Trp Ile Val Lys Asn Asn Lys Ile Ala Ile Val Arg Asp Lys
385 390 395 400
Val Gln Gly
<210> 382
<211> 374
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 382
Met Pro Glu Asn Asn Arg Tyr Tyr Asn Ile Ala Leu Lys Ser Asn Thr
1 5 10 15
Lys Lys Val Trp Asn Val Ser Gln Gly Ser Asn Asp Asn Asp Arg Ala
20 25 30
Ile Val Leu Trp Gln Gly Gly Ser Ala Asp His Glu Arg Phe Ala Phe
35 40 45
Phe Gln Leu Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly
50 55 60
Lys Val Val Thr Phe Gly Thr Gly Ser Trp Val Ser Gly Gly Pro Ile
65 70 75 80
Pro Asp Ser Glu Thr Leu Gln Gln Gln Lys Trp Thr Gly Glu Ala Lys
85 90 95
Gln Lys Trp Asn Leu Arg Asp Leu Gly Ser Asp Gly His Gly Ser Asn
100 105 110
Asn Tyr Glu Ile Val Asn Gln Ala Asn Gly Lys Val Pro Ser Tyr Arg
115 120 125
Trp Ile Gly Ser Thr Val Asp Asn Thr Glu Tyr Leu Lys Leu Asp Asn
130 135 140
Ser Asn Pro Ser Asp Ala Asp Lys Val Phe Thr Leu Phe Asn Gly Thr
145 150 155 160
Met Asn Leu Trp Gly Gln Asn Ile Thr Phe Phe Asn Asp Thr Ile Ser
165 170 175
Val Pro Lys Leu Pro Ala Ile Gly Thr Arg Pro Glu Ala Pro Val Tyr
180 185 190
Thr Gly Gly Ala Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val
195 200 205
Val Gly Ala Ser Leu Ile Pro Cys Phe Met Val Asn Asp Asn Gln Ala
210 215 220
Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys
225 230 235 240
Glu Glu Tyr Trp Asp Lys Thr Phe Ser Ala Val Ile Pro Gly Gly Leu
245 250 255
Lys Arg Asp Tyr Thr Phe Lys Ser Gly Met Thr Ser Val Asp Gln Lys
260 265 270
Lys Met Thr Asp Thr Leu Ser Met Lys Ile Gly Ala Asp Phe Gly Leu
275 280 285
Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Thr Glu Ile Ser Arg Thr
290 295 300
Leu Gln Thr Glu Ile Thr Ser Thr Asp Thr Glu Val Ser Glu Glu Thr
305 310 315 320
Val Ser Ser Ser Leu Thr Ser Pro Ala Gly Lys Thr Thr Gly Phe Thr
325 330 335
Glu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser
340 345 350
Ile Val Ser Asp Pro Trp Ile Val Lys Asn Asn Lys Ile Ala Ile Val
355 360 365
Arg Asp Lys Val Gln Gly
370
<210> 383
<211> 407
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 383
Met Lys Asn Ser Pro Tyr Lys Ile Leu Ser Ala Thr Ala Met Ile Ala
1 5 10 15
Ala Ile Thr Val Gly Asn Ile Ile Pro Ser Thr Pro Val Phe Ala Gln
20 25 30
Glu Gln Val Val His Glu Gln Gln Asn Ser Ala Tyr Ala Leu Gly Pro
35 40 45
Asn Gly Met Lys Asp Ala Leu Ala Lys Thr Gly Ser His Met Leu Val
50 55 60
Met Asn Val Tyr Ala Ser Thr Met Ile Lys Gln Pro Thr Val Asn Leu
65 70 75 80
Ser Gly Ile Asp Leu Gly Ala Asp Gly Glu Gln Leu Val Lys Lys Ile
85 90 95
Gln Asn Asp Gln Asn Val Ser Ile Ser Asn Ala Asn His Trp Met Asp
100 105 110
Val Glu Lys Pro Lys Ile Gln Lys Ala Ala Arg Ser Ile Val Asn Tyr
115 120 125
Asn Thr Gln Phe Gln Asn Tyr Tyr Gly Thr Ile Val Gly Ala Ala Lys
130 135 140
Gln Asn Asn Thr Pro Thr Leu Lys Glu Gly Leu Gln Asp Leu Tyr Thr
145 150 155 160
Thr Ile Asn Lys Asn Ser Lys Glu Val Asp Asp Val Ile Lys Ala Leu
165 170 175
Lys Glu Phe Lys Asn Lys Leu Tyr Thr Asp Ser Thr Gln Phe Lys Asn
180 185 190
Asp Val Asp Gly Ala Asp Gly Lys Ala Gly Leu Ala Thr Ile Leu Ala
195 200 205
Gly Lys Asn Ala Leu Ile Pro Gln Leu Lys Ser Glu Ile Glu Ser Leu
210 215 220
Arg Gly Ser Gln Lys Ala Tyr Leu Asp Asn Val Leu Gly Trp Gly Ile
225 230 235 240
Gly Gly Gly Val Gly Thr Phe Leu Leu Ile Gly Gly Ala Ile Gly Gly
245 250 255
Ala Val Ala Ile Val Val Thr Gly Gly Thr Ala Thr Pro Leu Ile Val
260 265 270
Gly Gly Leu Val Ala Leu Gly Ala Ala Gly Ile Gly Leu Gly Thr Ala
275 280 285
Thr Gly Val Met Leu Ser Gln Asn Leu Thr Gln Tyr Asn Asp Val Ser
290 295 300
Lys Lys Ile Glu Gln Leu Ser Thr Gln Arg Thr Ala Ala Glu Gln Ala
305 310 315 320
Val Leu Ser Leu Ala Asn Ala Lys Ala Thr Leu Met Asp Leu Tyr Gln
325 330 335
Thr Val Asp Gln Ala Ile Asp Ser Leu Thr Ala Met Lys Lys Gln Trp
340 345 350
Asp Thr Met Gly Ala Asn Tyr Lys Val Leu Leu Asp Ser Val Asp Ser
355 360 365
Met Gln Pro Ser Lys Val Ser Leu Leu Val Asn Asp Leu Asp Ala Ala
370 375 380
Lys Gln Gln Trp Ser Asp Ile Tyr Gln Ala Ala Asp Leu Val Ala Lys
385 390 395 400
Asp Ile Gly Phe Lys Asn Glu
405
<210> 384
<211> 377
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 384
Met Gln Glu Gln Val Val His Glu Gln Gln Asn Ser Ala Tyr Ala Leu
1 5 10 15
Gly Pro Asn Gly Met Lys Asp Ala Leu Ala Lys Thr Gly Ser His Met
20 25 30
Leu Val Met Asn Val Tyr Ala Ser Thr Met Ile Lys Gln Pro Thr Val
35 40 45
Asn Leu Ser Gly Ile Asp Leu Gly Ala Asp Gly Glu Gln Leu Val Lys
50 55 60
Lys Ile Gln Asn Asp Gln Asn Val Ser Ile Ser Asn Ala Asn His Trp
65 70 75 80
Met Asp Val Glu Lys Pro Lys Ile Gln Lys Ala Ala Arg Ser Ile Val
85 90 95
Asn Tyr Asn Thr Gln Phe Gln Asn Tyr Tyr Gly Thr Ile Val Gly Ala
100 105 110
Ala Lys Gln Asn Asn Thr Pro Thr Leu Lys Glu Gly Leu Gln Asp Leu
115 120 125
Tyr Thr Thr Ile Asn Lys Asn Ser Lys Glu Val Asp Asp Val Ile Lys
130 135 140
Ala Leu Lys Glu Phe Lys Asn Lys Leu Tyr Thr Asp Ser Thr Gln Phe
145 150 155 160
Lys Asn Asp Val Asp Gly Ala Asp Gly Lys Ala Gly Leu Ala Thr Ile
165 170 175
Leu Ala Gly Lys Asn Ala Leu Ile Pro Gln Leu Lys Ser Glu Ile Glu
180 185 190
Ser Leu Arg Gly Ser Gln Lys Ala Tyr Leu Asp Asn Val Leu Gly Trp
195 200 205
Gly Ile Gly Gly Gly Val Gly Thr Phe Leu Leu Ile Gly Gly Ala Ile
210 215 220
Gly Gly Ala Val Ala Ile Val Val Thr Gly Gly Thr Ala Thr Pro Leu
225 230 235 240
Ile Val Gly Gly Leu Val Ala Leu Gly Ala Ala Gly Ile Gly Leu Gly
245 250 255
Thr Ala Thr Gly Val Met Leu Ser Gln Asn Leu Thr Gln Tyr Asn Asp
260 265 270
Val Ser Lys Lys Ile Glu Gln Leu Ser Thr Gln Arg Thr Ala Ala Glu
275 280 285
Gln Ala Val Leu Ser Leu Ala Asn Ala Lys Ala Thr Leu Met Asp Leu
290 295 300
Tyr Gln Thr Val Asp Gln Ala Ile Asp Ser Leu Thr Ala Met Lys Lys
305 310 315 320
Gln Trp Asp Thr Met Gly Ala Asn Tyr Lys Val Leu Leu Asp Ser Val
325 330 335
Asp Ser Met Gln Pro Ser Lys Val Ser Leu Leu Val Asn Asp Leu Asp
340 345 350
Ala Ala Lys Gln Gln Trp Ser Asp Ile Tyr Gln Ala Ala Asp Leu Val
355 360 365
Ala Lys Asp Ile Gly Phe Lys Asn Glu
370 375
<210> 385
<211> 355
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 385
Met Ala Pro Ala Asn Asn Ala Tyr His Ser Ile His Leu Ala Ala Asp
1 5 10 15
Ala Thr Leu Lys Trp Asp Val Tyr Gly Asn Gly Thr Tyr Asp Glu Ala
20 25 30
Gly Ile Leu Leu Tyr Asn Gly Asn Ile Ser Asp Asn Glu Gln Phe Leu
35 40 45
Phe Phe Pro Leu Asp Gly Gly Leu Tyr Ala Ile Val Asn Lys Asn Ser
50 55 60
Gly Lys Ala Val Thr Ile Gly Gly Gly Trp Val Gly Asp Asn Ala Phe
65 70 75 80
Ser Tyr Asn Arg Glu Gly Leu Ile Gln Ser Lys Trp Thr Gly Ala Asn
85 90 95
Thr Asn Lys Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile
100 105 110
Val Asn Gln Gly Tyr Gly Lys Val Ala Ser Tyr Ala Arg Leu Gly Ile
115 120 125
Ser Gly Ala Lys Tyr Val Asp Leu Asp Asp Ser Asn Pro Ser Asp Asn
130 135 140
Asp Arg Leu Phe Asn Ile Pro Ala Thr Ala Arg Ser Thr Phe Ser Leu
145 150 155 160
Pro Thr Leu Pro Asn Val Gly Thr Arg Pro Asp Ala Pro Asn Tyr Thr
165 170 175
Gly Gly Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser Val Val Val
180 185 190
Gly Ala Ser Leu Leu Pro Cys Ile Met Val Asn Asp Ser Gln Ala Ser
195 200 205
Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu
210 215 220
Glu Tyr Trp Asp Arg Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser
225 230 235 240
Glu Ser Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys
245 250 255
Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ala Asp Leu Gly Ile Lys
260 265 270
Phe Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile
275 280 285
Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg
290 295 300
Ser Tyr Thr Ala Thr Gly Glu Ser Gly Lys Ala Thr Gly Tyr Thr Gln
305 310 315 320
Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile
325 330 335
Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg
340 345 350
Lys Thr Ser
355
<210> 386
<211> 288
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 386
Met Ala Glu Asn Thr Pro Thr Ser Val Asn Glu Asn Val Arg Val Gly
1 5 10 15
Ile Thr Asp Val Gln Ser Glu Leu Lys Lys Ile Gly Thr Tyr Tyr Tyr
20 25 30
Thr Asn Glu Ile Ala Gly Thr Lys Ile Glu Ser Trp Pro Thr Leu Thr
35 40 45
Phe Lys Ala Lys Pro Asp Ser Val Glu Thr Lys Val Asp Phe Ser Ile
50 55 60
Thr Asp Thr Thr Asn Lys Leu Asn Phe Asp Ser Lys Ile Val Glu Tyr
65 70 75 80
Met Gly Glu Asn Val Phe Glu Asn Thr Thr Asp Gln Thr His Lys Phe
85 90 95
Ser Thr Ala Lys Tyr Thr Lys Ser Val Thr Glu Ser Ile Ala Thr Ala
100 105 110
Thr Thr Lys Gly Phe Asn Val Gly Gly Ser Ser Asn Ile Phe Asn Ile
115 120 125
Pro Leu Ile Leu Asn Asn Gly Ile Thr Val Asn Ala Gly Phe Asn Ser
130 135 140
Ser Thr Thr Glu Thr Gln Thr Lys Ala Glu Gln Lys Thr Ile Glu Ala
145 150 155 160
Pro Ser Gln Ser Ile Glu Val Pro Pro Gln Lys Thr Tyr Lys Ala Asp
165 170 175
Val Ile Leu Glu Gln Arg Asn Phe Trp Gly Asp Ile Thr Phe Thr Gly
180 185 190
Val Gly Ser Asn Pro Val Thr Thr Ile Lys Gly Thr Ala Ser Tyr Trp
195 200 205
Ala Pro Asn Gly Met Gly Ala Trp Lys Gln Tyr Pro Phe Ser Asp Lys
210 215 220
Thr Lys Asn Tyr Trp Asn Lys Leu Thr Thr Ser Gln Lys Asn Asn Leu
225 230 235 240
Asn Gly Ile Lys Phe Glu Asn Asn Asp Val Lys Ala Glu Gly Thr Ala
245 250 255
Lys Val Glu Gly Ile Phe Gly Ser Asn Leu Ile Val Lys Val Tyr Asp
260 265 270
Ile Thr Asp Lys Ser Asn Pro Lys Leu Val Glu Thr Arg Ser Phe Lys
275 280 285
<210> 387
<211> 294
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 387
Met Ala Ile Val Asp Leu Asp Ala Tyr Leu Val Glu Leu Phe Lys Lys
1 5 10 15
Ile Met Tyr Pro Ser Pro Thr Arg Glu Asp Thr Thr Asn Leu Thr Val
20 25 30
Lys His Ala Glu Ser Tyr Gly Phe Glu Ile Lys Ala Asn Lys Pro Gln
35 40 45
Gly Thr Leu Phe Met Gly Glu Ala Val Leu Glu Asn Lys Thr Asn Glu
50 55 60
Thr Gln Thr Leu Lys Ser Asp Ser Phe Thr Lys Thr Val Thr Asp Ser
65 70 75 80
Ile Thr Leu Ser Val Ile Asn Gly Ile Lys Thr Gly Val Asn Ile Gly
85 90 95
Ile Ser Gly Lys Val Phe Gly Ile Gly Val Glu Ser Ser Met Ser Phe
100 105 110
Glu Val Ser Thr Ser Thr Thr Arg Glu Gln Thr Ser Thr Glu Gly Val
115 120 125
Lys Tyr Thr Val Pro Ser Gln Asn Val Val Val Pro Ala Lys Lys Thr
130 135 140
Tyr Tyr Val Tyr Thr Ser Leu Gln Arg Ser Asn Leu Glu Gly Thr Val
145 150 155 160
Arg Leu Arg Ala Asp Leu Ser Gly Pro Ile Leu Ile Met Leu Lys Phe
165 170 175
Gly Ser Thr Glu Ile Pro Val Tyr Ala Gly Asp Ile Phe Glu Thr Leu
180 185 190
Asn Gly Tyr Gln Phe Leu His Pro Leu Pro Ser Gly Ile Ser Leu Asn
195 200 205
His Lys Asp Lys Ser Ile His Phe Glu Gly Ile Ala Glu Tyr Ile Tyr
210 215 220
Gly Thr Gly Thr Lys Phe Tyr Val Thr Val Thr Asp Thr Pro Ser Ser
225 230 235 240
Gln Gly Thr Leu Glu His Lys Pro Phe Asp Ala Lys Thr Gly Leu Gly
245 250 255
Thr Tyr Glu Leu Leu Leu Asn Gly Lys Lys Pro Val Leu Asp Leu Asn
260 265 270
Asp Leu Lys Glu His Leu Glu Pro Lys Asp Phe Glu Lys Leu Lys Lys
275 280 285
Leu Gln Asn Glu Ile Val
290
<210> 388
<211> 311
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 388
Met Ala Ile Thr Asp Ile Glu Ser Ala Ala Lys Asp Tyr Met Asn Trp
1 5 10 15
Ala Leu Pro His Tyr Ser Gly Tyr Ser Pro Thr Ser Tyr Lys Phe Tyr
20 25 30
Pro Tyr Thr Ile Ser Asn Val Ser Ala Val Pro Ala Gln Thr Ala Phe
35 40 45
Glu Thr Ala Pro Val Gln Lys Ala Ala Ser Val Gln Val Leu Thr Asn
50 55 60
Asn Thr Ser Leu Thr Gln Ser Gln Thr Leu Lys Phe Ser Glu Lys Leu
65 70 75 80
Ile Glu Ser Thr Thr Gly Ser Thr Thr Glu Gly Tyr Lys Ile Gly Ser
85 90 95
Gly Ile Thr Ser Thr Ser Lys Phe Ser Tyr Asn Val Lys Ile Phe Ala
100 105 110
Ala Asn Ile Gly Phe Asp Gln Thr Phe Thr Val Thr Thr Thr Gly Glu
115 120 125
Tyr Asn His Ser Ser Thr Glu Thr Thr Thr Lys Thr Thr Glu Lys Leu
130 135 140
Trp Glu Val Thr Gln Pro Val Thr Val Pro Pro Tyr Thr Gln Ile Val
145 150 155 160
Ala Thr Leu Ile Ile Leu Gly Ala Asp Leu Gln Ile Pro Met Arg Leu
165 170 175
Asn Ala Thr Leu Ile Gly Asn Gly Thr Glu Phe Gly Ala Glu Asn Leu
180 185 190
Tyr Cys Ala Ala Tyr Phe Tyr Asn Pro Asn Tyr Met Val Gln Pro Tyr
195 200 205
Tyr Ala Gly Asn Met Ala Asp Arg Ala Trp Pro Asn Arg Pro Ala Ser
210 215 220
Phe Ile Gln Gly Val Tyr Ser Leu Val Leu Gln Gly Ala Ser Thr Thr
225 230 235 240
Ser His Gly Pro Gly Leu Tyr Ala Ala Val Leu Phe Glu Gln Thr Pro
245 250 255
Leu Pro Gly Phe Glu Thr Phe Ser Glu Ser Lys Thr Trp Tyr Ser Asn
260 265 270
Glu Val Leu Leu Arg Asp Gly Ser Ile Ile Thr Ile Pro Asp Leu Asp
275 280 285
Pro Lys Thr Gly Arg Ala Leu Pro Ala Lys Val Leu Glu Pro Val Met
290 295 300
Val Ser Arg Ser Ser Asn Asp
305 310
<210> 389
<211> 407
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 389
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Gly Val Gly Val
1 5 10 15
Met Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Ala Pro Glu
20 25 30
Asn Asn Arg Tyr Tyr Ser Ile His Leu Lys Ala Asn Pro Lys Leu Ala
35 40 45
Trp Asp Val Tyr Lys Gly Trp Asp Ser Asp Asn Ala Gly Ile Val Leu
50 55 60
Tyr Asn Gly Ser His Gly Asp Asn Glu Gln Phe Thr Phe Phe Pro Leu
65 70 75 80
Asp Gly Gly Ala Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro Val
85 90 95
Gly Leu Gly Asn Ala Tyr Ile Ser Thr Asp Gly Val Arg Asp Gly Phe
100 105 110
Ser Gly Asn Asn Gln Gly Leu Arg Gln Thr Asn Trp Thr Gly Ala Ser
115 120 125
Asn Glu Gln Trp Tyr Leu Arg Asp Lys Gly Asn Asn Asn Tyr Glu Ile
130 135 140
Val Asn Gln Gly Thr Gly Lys Val Ala Ser Trp Ser Trp Asn Gly Ile
145 150 155 160
Ser Gly Pro His Ser Ala Glu Tyr Val Asp Leu Asp Glu Ser Asn Pro
165 170 175
Ser Asp Asn Asn Arg Val Phe Asn Ile Ala Lys Asn Gly Leu Gly Gly
180 185 190
Ile Gly Val Ser Ile Pro Gly Phe Asp Ile Asp Tyr Gly Thr Phe Ser
195 200 205
Leu Pro Thr Leu Pro Ala Val Gly Thr Arg Pro Asn Ala Pro Asp Tyr
210 215 220
Asn Thr Ser Gly Ala Ile Asp Gln Gln Leu Pro Gln Thr Ser Asn Ser
225 230 235 240
Val Val Val Gly Ala Ser Leu Ile Pro Ser Ile Met Val Lys Asp Asn
245 250 255
Gly Ala Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Ala Leu
260 265 270
Val Lys Glu Glu Tyr Trp Glu Lys Val Arg Ser Glu Ile Ile Pro Ala
275 280 285
Gly Ala Thr Ser Lys Tyr Thr Val Lys Ser Gly Val Ser Glu Ile Asp
290 295 300
Gln Lys Lys Met Ser Asp Thr Leu Ala Met Ser Phe Gly Ser Asp Leu
305 310 315 320
Gly Leu Lys Phe Gly Glu Ser Ser Leu Ser Ile Lys Ser Ser Val Ser
325 330 335
Arg Thr Ile Gln Thr Glu Val Thr Lys Thr Ser Thr Asn Ala Thr Glu
340 345 350
Glu Thr Glu Glu Lys Ser Ile Pro Ser Ile Pro Gly Lys Asn Thr Gly
355 360 365
Phe Thr Ala Tyr Gln Leu Val Thr Lys Tyr Thr Leu Lys Arg Thr Asp
370 375 380
Gly Ser Val Val Thr Asp Pro Ser Ile Val Arg Asp Ser Lys Gln Thr
385 390 395 400
Ile Ala Arg Thr Ile Ala Asn
405
<210> 390
<211> 405
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 390
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Ala Val Gly Ala
1 5 10 15
Met Ser Leu Ser Ile Trp Ala Pro Ala Ser Gln Ala Asp Ser Pro Glu
20 25 30
Lys Asn Arg Tyr Tyr Ser Ile His Leu Lys Ser Asn Pro Lys Leu Ala
35 40 45
Trp Asp Val Asn Asp Asn Ser Pro Asp Asp Asn Arg Ala Ile Leu Leu
50 55 60
Trp Asn Thr Ser Ser Arg Gly Asp Asn Glu Gln Phe Val Phe Phe Pro
65 70 75 80
Leu Asp Gly Gln Asn Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys Pro
85 90 95
Val Thr Phe Gly Gly Ser Ser Pro Ala Tyr Ile Gly Asp Arg Asp Ala
100 105 110
Leu Leu Ser Asn Arg Glu Ala Leu Gln Gln Arg Ser Trp Thr Gly Ser
115 120 125
Pro Thr Glu Gln Trp Tyr Leu Arg Asp Lys Gly Ser Asn Asn Tyr Glu
130 135 140
Ile Val Asn Gln Gly Asn Gln Lys Val Ala Ser Tyr Ala Tyr Thr Trp
145 150 155 160
Ala Ser Arg His Val Glu Tyr Val Asp Leu Asp Glu Ser Asn Pro Ser
165 170 175
Asp Ser Asp Lys Val Phe Asn Ile Ala Lys Asn Gly Leu Gly Gly Leu
180 185 190
Gly Val Ser Ile Pro Gly Phe Asp Ile Asp Tyr Gly Thr Phe Ser Leu
195 200 205
Pro Thr Leu Pro Ala Thr Gly Thr Arg Pro Thr Ala Pro Glu Tyr Asn
210 215 220
Lys Asp Ala Ala Asp Pro Asn Gln Gln Leu Pro Gln Thr Ser Asn Ser
225 230 235 240
Val Val Val Gly Ala Ser Leu Val Pro Cys Ile Met Val Thr Asp Ser
245 250 255
Gln Thr Ser Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu
260 265 270
Ile Lys Glu Glu Tyr Trp Asp Lys Ala Ala Ser Thr Val Leu Ala Pro
275 280 285
Gly Ser Thr Gln Ser Val Ala Phe Lys Thr Gly Met Ser Ser Ser Asp
290 295 300
Gln Gln Lys Met Thr Glu Thr Ile Ser Met Lys Ile Gly Ala Asp Phe
305 310 315 320
Gly Leu Lys Phe Leu Asp Lys Ile Gly Pro Leu Lys Ala Glu Ile Ser
325 330 335
Lys Thr Leu Gln Thr Glu Thr Ser Thr Ser Asn Thr Glu Leu Ser Glu
340 345 350
Asp Thr Val Thr Thr Asn Leu Thr Gly Glu Ala Gly Lys Thr Thr Gly
355 360 365
Tyr Thr Leu Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp
370 375 380
Gly Ser Glu Val Ser Gly Ser Trp Thr Val Lys Asn Asn Lys Ile Thr
385 390 395 400
Lys Ile Ser Lys Ser
405
<210> 391
<211> 373
<212> БЕЛОК
<213> Bacillus sp.
<400> 391
Val Gly Lys Ser Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys
1 5 10 15
Asn Lys Asn Ser Arg Lys Tyr Leu Asp Ile Ser Gly Asn Glu Thr Gly
20 25 30
Asn Asn Ala Asn Val Gln Gln Tyr Glu Tyr Leu Ala Asp Ala Pro Ser
35 40 45
Glu Arg Phe Phe Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile
50 55 60
Asn Leu Asn Ser Gly Lys Val Ile Asp Ile Ser Gly Asn Glu Thr Gly
65 70 75 80
Asn Asn Ala Asn Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ala Pro Ser
85 90 95
Glu Tyr Trp Tyr Phe His Arg Glu Ala Asp Gly Tyr Tyr Val Ile Glu
100 105 110
Ser Lys His Ser Gly Lys Val Leu Asp Ile Ser Gly Asn Glu Thr Gly
115 120 125
Asn Asn Ala Asn Val Gln Gln Tyr Glu Phe Leu Thr Asp Ala Pro Ser
130 135 140
Glu Arg Phe Ala Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile
145 150 155 160
Asn Thr Gln Pro Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp
165 170 175
Gln Leu Pro Glu Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val
180 185 190
Pro Ala Ile Ser Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys
195 200 205
Gln Ile Lys Glu Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp
210 215 220
Lys Lys Gln Glu Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Arg Tyr
225 230 235 240
Thr Ser Lys Thr Gly Ile Lys Ile Thr Asp Gln Glu Thr Ala Thr Lys
245 250 255
Thr Val Ser Trp Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly
260 265 270
Phe Ser Val Gly Met Ser Ser Gln Tyr Ser Thr Gln Leu Gln Thr Ser
275 280 285
Ile Ser His Thr Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu
290 295 300
Ile Lys Asn Pro Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu
305 310 315 320
Thr Thr Glu Tyr Ser Val Gln Arg Lys Asn Gly Thr Ile Val Asn Ser
325 330 335
Pro Trp Thr Met Thr Asp Lys Thr Lys Thr His Ala Val Thr Tyr Pro
340 345 350
Asn Ala Glu Glu Lys Ala Leu Asn Asp Asn Thr Lys Gln Leu Ser Lys
355 360 365
Ala Glu Ser Val Asn
370
<210> 392
<211> 369
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 392
Met Thr Phe Lys Val Gly Met Lys Tyr Met Phe Lys Asn Lys Asn Ser
1 5 10 15
Arg Lys Tyr Leu Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
20 25 30
Val Gln Gln Tyr Glu Tyr Leu Ala Asp Ala Pro Ser Glu Arg Phe Phe
35 40 45
Leu His Pro Leu Asp Asn Asn Tyr Tyr Ala Met Ile Asn Leu Asn Ser
50 55 60
Gly Lys Val Ile Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
65 70 75 80
Ile Gln Gln Tyr Glu Trp Leu Gly Asp Ala Pro Ser Glu Tyr Trp Tyr
85 90 95
Phe His Arg Glu Ala Asp Gly Tyr Tyr Val Ile Glu Ser Lys His Ser
100 105 110
Gly Lys Val Leu Asp Ile Ser Gly Asn Glu Thr Gly Asn Asn Ala Asn
115 120 125
Val Gln Gln Tyr Glu Phe Leu Thr Asp Ala Pro Ser Glu Arg Phe Ala
130 135 140
Val Glu Glu Ala Gly Ser Val Ser Leu Pro Ser Ile Asn Thr Gln Pro
145 150 155 160
Leu Ser Pro Val Pro Gln Tyr Glu Thr Ile Asn Asp Gln Leu Pro Glu
165 170 175
Glu Thr Glu Arg Val Val Thr Ala Phe Thr Ile Val Pro Ala Ile Ser
180 185 190
Val Lys Asp Pro His Tyr Gly Gly Asp Thr Ala Lys Gln Ile Lys Glu
195 200 205
Asn Pro Tyr Tyr Met Val Val Lys Lys Gln Trp Trp Lys Lys Gln Glu
210 215 220
Ser Tyr Val Leu Ala Pro Gly Glu Thr Tyr Arg Tyr Thr Ser Lys Thr
225 230 235 240
Gly Ile Lys Ile Thr Asp Gln Glu Thr Ala Thr Lys Thr Val Ser Trp
245 250 255
Ser Ile Gly Ala Asp Met Gly Phe Ser Phe Lys Gly Phe Ser Val Gly
260 265 270
Met Ser Ser Gln Tyr Ser Thr Gln Leu Gln Thr Ser Ile Ser His Thr
275 280 285
Thr Glu Gln Leu Lys Glu Glu Thr Trp Asp His Glu Ile Lys Asn Pro
290 295 300
Ser Ser Asn Arg Met Ala Tyr Ser Arg Tyr Ile Leu Thr Thr Glu Tyr
305 310 315 320
Ser Val Gln Arg Lys Asn Gly Thr Ile Val Asn Ser Pro Trp Thr Met
325 330 335
Thr Asp Lys Thr Lys Thr His Ala Val Thr Tyr Pro Asn Ala Glu Glu
340 345 350
Lys Ala Leu Asn Asp Asn Thr Lys Gln Leu Ser Lys Ala Glu Ser Val
355 360 365
Asn
<210> 393
<211> 386
<212> БЕЛОК
<213> Bacillus sp.
<400> 393
Met Lys Ser Ile Ser Lys Lys Val Met Ala Gly Leu Leu Val Gly Ala
1 5 10 15
Thr Ser Leu Ser Ile Trp Ala Pro Ser Ser Gln Ala Ala Thr Pro Glu
20 25 30
Lys Asn Gly Tyr Tyr Ser Ile His Leu Ala Ser Asn Thr Trp Lys Lys
35 40 45
Trp Asp Val Ala Val Ala Ser Leu Asp Asn Gly Ala Gly Ile Leu Leu
50 55 60
Tyr Asp Val Ser Gly Lys Ile Pro Asp Asn Gln Gln Phe Ala Phe Phe
65 70 75 80
Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn Lys Asn Ser Gly Lys
85 90 95
Pro Val Thr Phe Gly Asp Gly Val Pro Pro Met Asp Gly Leu Gly Gly
100 105 110
Asn Ser Gly Ala Leu Lys Gln Thr Asn Trp Thr Gly Asn Ala Asn Glu
115 120 125
Lys Trp Tyr Leu Arg Asp Leu Gly Gly Asn Asp Phe Glu Ile Val Asn
130 135 140
Gln Gly Thr Gly Lys Val Ala Ser Tyr Ala Trp Val Gly Asp Leu Val
145 150 155 160
Arg His Tyr Glu Tyr Val Asp Leu Asp Asn Ala Asn Pro Ser Asp Arg
165 170 175
Asp Arg Val Phe His Ile Pro Ala Ala Arg Ser Thr Phe Thr Val Pro
180 185 190
Thr Leu Pro Ala Thr Gly Thr Arg Pro Val Ala Pro Asn Tyr Thr Gly
195 200 205
Gly Ile Asp Glu Gln Leu Pro Gln Thr Ser Lys Ser Val Ile Val Gly
210 215 220
Ala Ser Leu Ile Pro Cys Ile Met Val Lys Asp Ser Gln Thr Asn Asp
225 230 235 240
Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr Leu Val Lys Glu Glu
245 250 255
Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln Pro Gly Leu Ser Glu
260 265 270
Thr Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val Asp Gln Gln Lys Met
275 280 285
Thr Asp Thr Leu Ser Met Lys Phe Gly Ala Asp Phe Gly Ile Lys Phe
290 295 300
Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile Ser Arg Thr Ile Gln
305 310 315 320
Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr Glu Glu Thr Arg Ser
325 330 335
Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr Gly Tyr Thr Gln Tyr
340 345 350
Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala Asp Gly Ser Ile Val
355 360 365
Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile Thr Val Thr Arg Lys
370 375 380
Thr Ser
385
<210> 394
<211> 359
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 394
Met Ala Thr Pro Glu Lys Asn Gly Tyr Tyr Ser Ile His Leu Ala Ser
1 5 10 15
Asn Thr Trp Lys Lys Trp Asp Val Ala Val Ala Ser Leu Asp Asn Gly
20 25 30
Ala Gly Ile Leu Leu Tyr Asp Val Ser Gly Lys Ile Pro Asp Asn Gln
35 40 45
Gln Phe Ala Phe Phe Pro Leu Asp Gly Gly Thr Tyr Ala Ile Val Asn
50 55 60
Lys Asn Ser Gly Lys Pro Val Thr Phe Gly Asp Gly Val Pro Pro Met
65 70 75 80
Asp Gly Leu Gly Gly Asn Ser Gly Ala Leu Lys Gln Thr Asn Trp Thr
85 90 95
Gly Asn Ala Asn Glu Lys Trp Tyr Leu Arg Asp Leu Gly Gly Asn Asp
100 105 110
Phe Glu Ile Val Asn Gln Gly Thr Gly Lys Val Ala Ser Tyr Ala Trp
115 120 125
Val Gly Asp Leu Val Arg His Tyr Glu Tyr Val Asp Leu Asp Asn Ala
130 135 140
Asn Pro Ser Asp Arg Asp Arg Val Phe His Ile Pro Ala Ala Arg Ser
145 150 155 160
Thr Phe Thr Val Pro Thr Leu Pro Ala Thr Gly Thr Arg Pro Val Ala
165 170 175
Pro Asn Tyr Thr Gly Gly Ile Asp Glu Gln Leu Pro Gln Thr Ser Lys
180 185 190
Ser Val Ile Val Gly Ala Ser Leu Ile Pro Cys Ile Met Val Lys Asp
195 200 205
Ser Gln Thr Asn Asp Tyr Thr Lys Ile His Asn Ser Pro Tyr Tyr Thr
210 215 220
Leu Val Lys Glu Glu Tyr Trp Asp Lys Thr Phe Ser Lys Val Ile Gln
225 230 235 240
Pro Gly Leu Ser Glu Thr Tyr Ser Tyr Lys Thr Gly Val Ser Ser Val
245 250 255
Asp Gln Gln Lys Met Thr Asp Thr Leu Ser Met Lys Phe Gly Ala Asp
260 265 270
Phe Gly Ile Lys Phe Gly Asp Ala Ser Ala Ser Leu Lys Ser Glu Ile
275 280 285
Ser Arg Thr Ile Gln Thr Glu Ile Ser Thr Thr Asn Ser Glu Ala Thr
290 295 300
Glu Glu Thr Arg Ser Tyr Thr Ala Thr Gly Glu Pro Gly Lys Ala Thr
305 310 315 320
Gly Tyr Thr Gln Tyr Gln Leu Ala Thr Lys Tyr Thr Leu Lys Arg Ala
325 330 335
Asp Gly Ser Ile Val Ser Asp Pro Trp Val Val Lys Asn Asp Lys Ile
340 345 350
Thr Val Thr Arg Lys Thr Ser
355
<210> 395
<211> 425
<212> БЕЛОК
<213> Ensifer sp.
<400> 395
Val Arg Leu Gly Ser Phe Phe Gly Thr Gly Gly Leu Leu Pro Ser Gln
1 5 10 15
Val Glu His Cys Ser Ser His Pro Ile Ala Arg Gly Ala Ser Ile Asp
20 25 30
Arg Arg Phe Arg Arg Arg Gln Lys Thr Val Ala Gln Leu Ser Asn Tyr
35 40 45
Leu Pro Asp Arg Lys Gly Lys Asp Ala Ala Arg Arg Ala Ala Leu Ser
50 55 60
Tyr Thr Arg Phe Gly Cys Asn Ser Ala Val Met Gln Gln Gly Asp Ala
65 70 75 80
Ala Met Asn Glu Val Leu Arg Asn Ser Ala Gly Gln Arg Ser His Asp
85 90 95
Ser Ala Arg Ala Leu Arg Pro Arg Arg Ser Pro Ile Val Phe Ile Asn
100 105 110
Ala Thr Gln Asp Glu Ala Glu Gln Phe Gln Glu Ile Val Ala Ile Asp
115 120 125
Asp Ile Asp Tyr Leu Thr Gln Ala Val Ala Leu Thr Ala Leu Phe Asn
130 135 140
Gly Ala Ile Asp Asn Thr Ser Gly Arg Phe Val Pro Gly Lys Ala Arg
145 150 155 160
Glu Leu Ile Ser Asn Tyr Asn Glu Ser Leu Asp Ser Asp Ser Gln Arg
165 170 175
Tyr Lys Ile Gly Ile Phe Asn Thr His Gln Thr Thr Leu Thr Gln Gln
180 185 190
Asn Ser Ala Val Ser Ala Met Ile Asn Gln Ile Leu Glu Thr Leu Lys
195 200 205
Thr Val Met Gly Val Ala Leu Gly Ala Ser Ser Val Gly Gln Met Thr
210 215 220
Ala Ala Val Thr Asp Ala Phe Thr Asn Leu Asp Ser Gln Glu Gly Asp
225 230 235 240
Ala Trp Ile Phe Trp Glu Lys Lys Thr Ser Asn Lys Thr Thr Tyr Ser
245 250 255
Tyr Ala Ile Leu Phe Ala Phe Gln Asp Ser Thr Thr Gly Lys Leu Met
260 265 270
Phe Ala Leu Pro Met Ser Leu Glu Ile Glu Val Asp Val Ser Tyr Glu
275 280 285
Arg Val Leu Phe Ile Thr Val Glu Asp Lys Glu Thr Tyr Ser Val Lys
290 295 300
Leu Asp Thr Met Lys Val Gly Gln Leu Leu Phe Pro Lys Thr Pro Gly
305 310 315 320
Ala Gln Ala Leu Gln Ser Ala Arg Gln Leu Gly Thr Arg Ser Gly Ser
325 330 335
Val Asp Leu Leu Ala Asn Pro Ser Pro Val Thr Asn Ile Val Val Thr
340 345 350
Asn Trp Ala Lys Thr Lys Thr Phe Ala Thr Ala Ala Ala Gly Phe Tyr
355 360 365
Thr Ser Asn Tyr Pro Leu Val Gln Val Met Ala Glu Ala Pro Asn Val
370 375 380
Ile His Pro Val Phe Asp Gly Asp Lys Tyr Leu Val Ser Phe Asn Leu
385 390 395 400
Asn Gly Val Gln Gln Thr Ala Gly Phe Leu Leu Asn Gly Thr Met Pro
405 410 415
Asp Gly Glu Leu Trp Phe Val Ser Ile
420 425
<210> 396
<211> 344
<212> БЕЛОК
<213> Искусственная последовательность
<220>
<223> вариант нативной последовательности
<400> 396
Met Asn Glu Val Leu Arg Asn Ser Ala Gly Gln Arg Ser His Asp Ser
1 5 10 15
Ala Arg Ala Leu Arg Pro Arg Arg Ser Pro Ile Val Phe Ile Asn Ala
20 25 30
Thr Gln Asp Glu Ala Glu Gln Phe Gln Glu Ile Val Ala Ile Asp Asp
35 40 45
Ile Asp Tyr Leu Thr Gln Ala Val Ala Leu Thr Ala Leu Phe Asn Gly
50 55 60
Ala Ile Asp Asn Thr Ser Gly Arg Phe Val Pro Gly Lys Ala Arg Glu
65 70 75 80
Leu Ile Ser Asn Tyr Asn Glu Ser Leu Asp Ser Asp Ser Gln Arg Tyr
85 90 95
Lys Ile Gly Ile Phe Asn Thr His Gln Thr Thr Leu Thr Gln Gln Asn
100 105 110
Ser Ala Val Ser Ala Met Ile Asn Gln Ile Leu Glu Thr Leu Lys Thr
115 120 125
Val Met Gly Val Ala Leu Gly Ala Ser Ser Val Gly Gln Met Thr Ala
130 135 140
Ala Val Thr Asp Ala Phe Thr Asn Leu Asp Ser Gln Glu Gly Asp Ala
145 150 155 160
Trp Ile Phe Trp Glu Lys Lys Thr Ser Asn Lys Thr Thr Tyr Ser Tyr
165 170 175
Ala Ile Leu Phe Ala Phe Gln Asp Ser Thr Thr Gly Lys Leu Met Phe
180 185 190
Ala Leu Pro Met Ser Leu Glu Ile Glu Val Asp Val Ser Tyr Glu Arg
195 200 205
Val Leu Phe Ile Thr Val Glu Asp Lys Glu Thr Tyr Ser Val Lys Leu
210 215 220
Asp Thr Met Lys Val Gly Gln Leu Leu Phe Pro Lys Thr Pro Gly Ala
225 230 235 240
Gln Ala Leu Gln Ser Ala Arg Gln Leu Gly Thr Arg Ser Gly Ser Val
245 250 255
Asp Leu Leu Ala Asn Pro Ser Pro Val Thr Asn Ile Val Val Thr Asn
260 265 270
Trp Ala Lys Thr Lys Thr Phe Ala Thr Ala Ala Ala Gly Phe Tyr Thr
275 280 285
Ser Asn Tyr Pro Leu Val Gln Val Met Ala Glu Ala Pro Asn Val Ile
290 295 300
His Pro Val Phe Asp Gly Asp Lys Tyr Leu Val Ser Phe Asn Leu Asn
305 310 315 320
Gly Val Gln Gln Thr Ala Gly Phe Leu Leu Asn Gly Thr Met Pro Asp
325 330 335
Gly Glu Leu Trp Phe Val Ser Ile
340
<210> 397
<211> 296
<212> БЕЛОК
<213> Bacillus sp.
<400> 397
Met Ala Ile Val Asp Leu Asp Ala Tyr Leu Leu Ala Leu Ala Lys Lys
1 5 10 15
Ala His Pro Leu Gly Ser Leu Phe His Ala Val Ser Thr Arg Gln Leu
20 25 30
Ser Val Glu Asn Ala Glu Ser Tyr Gly Phe Arg Ile Glu Asn Ser Lys
35 40 45
Pro Gln Gly Thr Met Phe Met Gly Glu Ser Glu Leu Lys Asn Asp Thr
50 55 60
Asn Glu Thr Gln Thr Ile His Ser Asp Ser Phe Thr Lys Thr Ile Thr
65 70 75 80
Asp Ser Val Thr Leu Ser Val Thr Asn Gly Val Thr Ala Gly Val Ser
85 90 95
Val Thr Ile Ser Gly Lys Ile Phe Gly Ile Gly Ala Glu Ser Ser Met
100 105 110
Ser Phe Glu Val Ser Thr Ser Thr Thr Asn Glu Gln Thr Ser Glu Glu
115 120 125
Ser Val Ala Tyr Thr Val Pro Ser Gln Thr Val Val Val Pro Ala Lys
130 135 140
Lys Thr Tyr Tyr Val Tyr Ser Ala Leu Gln Arg Ser Leu Leu Glu Gly
145 150 155 160
Ser Ile Arg Leu Arg Ala Asp Leu Ser Gly Glu Val Tyr Ala Arg Met
165 170 175
Lys Ile Gly Asp Ile Ile Thr Ser Ile Leu Leu Gly Asp Ile Tyr Glu
180 185 190
Phe Ile Lys Thr His Gln Leu Leu His Pro Leu Pro Ser Gly Ile Ser
195 200 205
Leu Asn His Asn Asn Lys Ser Val His Phe Glu Gly Val Ala Gln Tyr
210 215 220
Leu Tyr Gly Thr Gly Thr Lys Phe Asn Val Thr Ile Thr Asp Thr Pro
225 230 235 240
Ser Ser Gln Gly Thr Gln Glu His Lys Ala Phe Asp Pro Lys Thr Gly
245 250 255
Leu Gly Thr Tyr Glu Ile Gln Leu Asp Gly Lys Lys Leu Ser Phe Asp
260 265 270
Leu Asn Asp Leu Lys Asp Gln Val Glu Pro Glu Val Phe Glu Lys Leu
275 280 285
Leu Glu Leu Lys Asn Glu Ile Val
290 295
<210> 398
<211> 296
<212> БЕЛОК
<213> Bacillus sp.
<400> 398
Met Ala Ile Val Asp Leu Asp Ala Tyr Leu Leu Ala Leu Ala Lys Lys
1 5 10 15
Ala His Pro Val Gly Ser Leu Phe Leu Ala Val Ser Thr Arg Gln Leu
20 25 30
Ser Val Glu Asn Ala Glu Ser Tyr Gly Phe Arg Ile Glu Asn Ser Lys
35 40 45
Pro Gln Gly Thr Met Phe Met Gly Glu Ser Glu Leu Lys Asn Asp Thr
50 55 60
Asn Glu Thr Gln Thr Ile His Ser Asp Ser Phe Thr Lys Thr Ile Thr
65 70 75 80
Asp Ser Val Thr Leu Ser Val Thr Asn Gly Val Thr Ala Gly Val Ser
85 90 95
Val Thr Ile Ser Gly Lys Ile Phe Gly Ile Gly Ala Glu Ser Ser Met
100 105 110
Ser Phe Glu Val Ser Thr Ser Thr Thr Asn Glu Gln Thr Ser Glu Glu
115 120 125
Ser Val Ala Tyr Thr Val Pro Ser Gln Thr Val Val Val Pro Ala Lys
130 135 140
Lys Thr Tyr Tyr Val Tyr Ser Ala Leu Gln Arg Ser Leu Leu Glu Gly
145 150 155 160
Ser Ile Arg Leu Arg Ala Asp Leu Ser Gly Glu Val Phe Ala Ser Met
165 170 175
Lys Phe Gly Asp Ala Ile Val Asn Ile Leu Ile Gly Asp Ile Tyr Glu
180 185 190
Phe Ile Lys Thr His Gln Leu Leu His Pro Leu Pro Ser Gly Ile Ser
195 200 205
Leu Asn His Asn Asn Lys Ser Val His Phe Glu Gly Val Ala Gln Tyr
210 215 220
Leu Tyr Gly Thr Gly Thr Lys Phe Asn Val Thr Ile Thr Asp Thr Pro
225 230 235 240
Ser Ser Gln Gly Thr Gln Glu His Lys Ala Phe Asp Pro Lys Thr Gly
245 250 255
Leu Gly Thr Tyr Glu Ile Gln Leu Asp Gly Lys Lys Leu Ser Phe Asp
260 265 270
Leu Asn Asp Leu Lys Asp Gln Val Glu Pro Glu Val Phe Glu Lys Leu
275 280 285
Leu Glu Leu Lys Asn Glu Ile Val
290 295
<---
Реферат
Изобретение относится к области биохимии, в частности к рекомбинантному полипептиду, обладающему пестицидной активностью против огневки кукурузной юго-западной и западного кукурузного жука, к способу его получения, а также к композиции, его содержащей. Также раскрыта рекомбинантная молекула нуклеиновой кислоты, кодирующая полипептид, обладающий пестицидной активностью против огневки кукурузной юго-западной и западного кукурузного жука, а также клетка-хозяин, конструкция ДНК, вектор, растение и семя растения, ее содержащие. Изобретение также относится к способу борьбы с популяцией огневки кукурузной юго-западной и западного кукурузного жука с использованием вышеуказанной композиции, а также к способу защиты растения от огневки кукурузной юго-западной и западного кукурузного жука и к способу повышения урожайности растения с использованием вышеуказанной молекулы нуклеиновой кислоты. Изобретение позволяет эффективно бороться с огневкой кукурузной юго-западной и западным кукурузным жуком. 14 н. и 25 з.п. ф-лы, 2 ил., 4 табл., 8 пр.
Формула
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Способы борьбы с вредителем - совкой agrotis
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